LOCUS       DACEJZ010000001        91235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEJZ010000001 DACEJZ010000000
VERSION     DACEJZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.001"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW656_000001"
     CDS             83..970
                     /locus_tag="IW656_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0835739.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW656_000002"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW656_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0835740.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW656_000003"
     CDS             2272..2718
                     /locus_tag="IW656_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0835741.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW656_000004"
     CDS             2991..4343
                     /locus_tag="IW656_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0835742.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW656_000005"
     CDS             4514..4801
                     /locus_tag="IW656_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835743.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW656_000006"
     CDS             5027..5470
                     /locus_tag="IW656_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0835744.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW656_000007"
     CDS             complement(5476..6660)
                     /locus_tag="IW656_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0835745.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW656_000008"
     CDS             6928..7626
                     /locus_tag="IW656_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0835746.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW656_000009"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW656_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0835747.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW656_000010"
     CDS             complement(8436..8969)
                     /locus_tag="IW656_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835748.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW656_000011"
     CDS             complement(9266..11647)
                     /locus_tag="IW656_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0835749.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW656_000012"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW656_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0835750.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12886
                     /locus_tag="IW656_000013"
                     /pseudo
     CDS             12348..12886
                     /locus_tag="IW656_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
     gene            12976..13395
                     /gene="gpsB"
                     /locus_tag="IW656_000014"
     CDS             12976..13395
                     /gene="gpsB"
                     /locus_tag="IW656_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0835751.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13417..13784
                     /gene="rnpB"
                     /locus_tag="IW656_000015"
     ncRNA           13417..13784
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW656_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13993..15159
                     /locus_tag="IW656_000016"
     CDS             13993..15159
                     /locus_tag="IW656_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0835752.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15205..16701
                     /locus_tag="IW656_000017"
     CDS             15205..16701
                     /locus_tag="IW656_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0835753.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16782..17465
                     /locus_tag="IW656_000018"
     CDS             16782..17465
                     /locus_tag="IW656_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0835754.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18110..19300
                     /locus_tag="IW656_000019"
     CDS             18110..19300
                     /locus_tag="IW656_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0835755.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19402..20355
                     /locus_tag="IW656_000020"
     CDS             19402..20355
                     /locus_tag="IW656_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0835756.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20424..21773
                     /locus_tag="IW656_000021"
     CDS             20424..21773
                     /locus_tag="IW656_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0835757.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21956..22279)
                     /locus_tag="IW656_000022"
     CDS             complement(21956..22279)
                     /locus_tag="IW656_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835758.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22408..23571
                     /locus_tag="IW656_000023"
     CDS             22408..23571
                     /locus_tag="IW656_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835759.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23641..24990
                     /locus_tag="IW656_000024"
     CDS             23641..24990
                     /locus_tag="IW656_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0835760.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25035..25466)
                     /locus_tag="IW656_000025"
     CDS             complement(25035..25466)
                     /locus_tag="IW656_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0835761.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25854..28496
                     /gene="alaS"
                     /locus_tag="IW656_000026"
     CDS             25854..28496
                     /gene="alaS"
                     /locus_tag="IW656_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0835762.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28696..29292
                     /locus_tag="IW656_000027"
     CDS             28696..29292
                     /locus_tag="IW656_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0835763.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29414..30562
                     /locus_tag="IW656_000028"
     CDS             29414..30562
                     /locus_tag="IW656_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0835764.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30600..31013)
                     /locus_tag="IW656_000029"
     CDS             complement(30600..31013)
                     /locus_tag="IW656_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0835765.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31092..31523
                     /locus_tag="IW656_000030"
     CDS             31092..31523
                     /locus_tag="IW656_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0835766.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31622..31855)
                     /locus_tag="IW656_000031"
     CDS             complement(31622..31855)
                     /locus_tag="IW656_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0835767.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32038..33708
                     /locus_tag="IW656_000032"
     CDS             32038..33708
                     /locus_tag="IW656_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0835768.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33855..34241
                     /locus_tag="IW656_000033"
     CDS             33855..34241
                     /locus_tag="IW656_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835769.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34228..34926
                     /locus_tag="IW656_000034"
     CDS             34228..34926
                     /locus_tag="IW656_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0835770.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34931..35395
                     /locus_tag="IW656_000035"
     CDS             34931..35395
                     /locus_tag="IW656_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0835771.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35392..36303
                     /locus_tag="IW656_000036"
     CDS             35392..36303
                     /locus_tag="IW656_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0835772.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36538..37077
                     /gene="pyrR"
                     /locus_tag="IW656_000037"
     CDS             36538..37077
                     /gene="pyrR"
                     /locus_tag="IW656_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0835773.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37091..38374
                     /locus_tag="IW656_000038"
     CDS             37091..38374
                     /locus_tag="IW656_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0835774.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38387..39313
                     /locus_tag="IW656_000039"
     CDS             38387..39313
                     /locus_tag="IW656_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0835775.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39332..40612
                     /locus_tag="IW656_000040"
     CDS             39332..40612
                     /locus_tag="IW656_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0835776.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40617..41693
                     /gene="carA"
                     /locus_tag="IW656_000041"
     CDS             40617..41693
                     /gene="carA"
                     /locus_tag="IW656_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0835777.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41698..44883
                     /gene="carB"
                     /locus_tag="IW656_000042"
     CDS             41698..44883
                     /gene="carB"
                     /locus_tag="IW656_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0835778.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44937..45713
                     /locus_tag="IW656_000043"
     CDS             44937..45713
                     /locus_tag="IW656_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0835779.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45710..46642
                     /locus_tag="IW656_000044"
     CDS             45710..46642
                     /locus_tag="IW656_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0835780.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46639..47355
                     /gene="pyrF"
                     /locus_tag="IW656_000045"
     CDS             46639..47355
                     /gene="pyrF"
                     /locus_tag="IW656_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0835781.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47362..47991
                     /locus_tag="IW656_000046"
     CDS             47362..47991
                     /locus_tag="IW656_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0835782.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47979..48293
                     /locus_tag="IW656_000047"
     CDS             47979..48293
                     /locus_tag="IW656_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835783.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48331..48972
                     /locus_tag="IW656_000048"
     CDS             48331..48972
                     /locus_tag="IW656_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0835784.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49003..49707
                     /locus_tag="IW656_000049"
     CDS             49003..49707
                     /locus_tag="IW656_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0835785.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49704..50606
                     /locus_tag="IW656_000050"
     CDS             49704..50606
                     /locus_tag="IW656_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0835786.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50764..52470)
                     /locus_tag="IW656_000051"
     CDS             complement(50764..52470)
                     /locus_tag="IW656_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0835787.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52660..53283
                     /locus_tag="IW656_000052"
     CDS             52660..53283
                     /locus_tag="IW656_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835788.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53733..54782
                     /locus_tag="IW656_000053"
     CDS             53733..54782
                     /locus_tag="IW656_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0835789.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54779..55660
                     /locus_tag="IW656_000054"
     CDS             54779..55660
                     /locus_tag="IW656_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0835790.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55663..56433
                     /locus_tag="IW656_000055"
     CDS             55663..56433
                     /locus_tag="IW656_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0835791.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56712..57566
                     /gene="aroE"
                     /locus_tag="IW656_000056"
     CDS             56712..57566
                     /gene="aroE"
                     /locus_tag="IW656_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0835792.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57744..58907
                     /locus_tag="IW656_000057"
     CDS             57744..58907
                     /locus_tag="IW656_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0835793.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59024..59434
                     /locus_tag="IW656_000058"
     CDS             59024..59434
                     /locus_tag="IW656_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0835794.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59608..61836
                     /gene="topB"
                     /locus_tag="IW656_000059"
     CDS             59608..61836
                     /gene="topB"
                     /locus_tag="IW656_000059"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0835795.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61974..62291
                     /locus_tag="IW656_000060"
     CDS             61974..62291
                     /locus_tag="IW656_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835796.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62451..62687
                     /locus_tag="IW656_000061"
     CDS             62451..62687
                     /locus_tag="IW656_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0835797.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62684..63082
                     /locus_tag="IW656_000062"
     CDS             62684..63082
                     /locus_tag="IW656_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0835798.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63749..64693)
                     /locus_tag="IW656_000063"
     CDS             complement(63749..64693)
                     /locus_tag="IW656_000063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0835799.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64989..65933)
                     /locus_tag="IW656_000064"
     CDS             complement(64989..65933)
                     /locus_tag="IW656_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0835800.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66154..67368
                     /locus_tag="IW656_000065"
     CDS             66154..67368
                     /locus_tag="IW656_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0835801.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67390..69405
                     /locus_tag="IW656_000066"
     CDS             67390..69405
                     /locus_tag="IW656_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0835802.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69488..70861)
                     /locus_tag="IW656_000067"
     CDS             complement(69488..70861)
                     /locus_tag="IW656_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0835803.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70887..71171)
                     /locus_tag="IW656_000068"
     CDS             complement(70887..71171)
                     /locus_tag="IW656_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0835804.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71173..73188)
                     /locus_tag="IW656_000069"
     CDS             complement(71173..73188)
                     /locus_tag="IW656_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0835805.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73367..73672)
                     /locus_tag="IW656_000070"
     CDS             complement(73367..73672)
                     /locus_tag="IW656_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0835806.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73761..74792)
                     /locus_tag="IW656_000071"
     CDS             complement(73761..74792)
                     /locus_tag="IW656_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0835807.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            74999..75874
                     /locus_tag="IW656_000072"
     CDS             74999..75874
                     /locus_tag="IW656_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0835808.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76026..76868)
                     /locus_tag="IW656_000073"
     CDS             complement(76026..76868)
                     /locus_tag="IW656_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0835809.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76960..78837)
                     /locus_tag="IW656_000074"
     CDS             complement(76960..78837)
                     /locus_tag="IW656_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0835810.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78834..80255)
                     /locus_tag="IW656_000075"
     CDS             complement(78834..80255)
                     /locus_tag="IW656_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0835811.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80258..83647)
                     /locus_tag="IW656_000076"
     CDS             complement(80258..83647)
                     /locus_tag="IW656_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0835812.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83904..85296
                     /locus_tag="IW656_000077"
                     /pseudo
     CDS             83904..85296
                     /locus_tag="IW656_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(85369..86016)
                     /locus_tag="IW656_000078"
     CDS             complement(85369..86016)
                     /locus_tag="IW656_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0835813.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86024..86710)
                     /locus_tag="IW656_000079"
     CDS             complement(86024..86710)
                     /locus_tag="IW656_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0835814.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86716..87789)
                     /locus_tag="IW656_000080"
     CDS             complement(86716..87789)
                     /locus_tag="IW656_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0835815.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88011..88685
                     /locus_tag="IW656_000081"
     CDS             88011..88685
                     /locus_tag="IW656_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0835816.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88690..90030
                     /locus_tag="IW656_000082"
     CDS             88690..90030
                     /locus_tag="IW656_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0835817.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90179..91165)
                     /locus_tag="IW656_000083"
     CDS             complement(90179..91165)
                     /locus_tag="IW656_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0835818.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        29100 a        16206 c        18614 g        27315 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa caattagcag aacaattagc
    12661 agattatgtc gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca
    12721 cactcgtttt atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc
    12781 tggaaaaacg aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga
    12841 aattcggctg atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt
    12901 gatttatcaa cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa
    12961 actagaggtg taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa
    13021 ttcaaaacaa aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc
    13081 atcaaagatt atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga
    13141 ttgagtgcaa aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa
    13201 caaacagagg caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca
    13261 aatcttgaac gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca
    13321 gcacaaccta atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa
    13381 acacgtcaat tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac
    13441 ttgatcaggc tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct
    13501 tagcgaaacc ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt
    13561 cggctatgct taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc
    13621 cggtggaacg cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg
    13681 tctggaagaa gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt
    13741 tttttctccc tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg
    13801 gaattaggtc gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat
    13861 ttttcaatac ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat
    13921 tcggaatttg agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata
    13981 aggagattgt gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg
    14041 ctagaagcac tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac
    14101 ggacgtgcac gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt
    14161 acagcagatc gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg
    14221 tttgaaaacg taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct
    14281 gtagctggaa aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca
    14341 aaaaaagcaa tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg
    14401 acggaaactg gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta
    14461 actctggata cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga
    14521 gcaccattga aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac
    14581 cgtccatttt atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata
    14641 ggccataata tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc
    14701 agtcaagaaa tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt
    14761 gaattagata ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat
    14821 gcagaagaaa tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc
    14881 aaaacggata aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga
    14941 gaagaagaaa ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa
    15001 acatggagta aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa
    15061 gcaaccaaaa aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg
    15121 ggaactcgtc cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca
    15181 attggatgtt tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa
    15241 atcaatcttc tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct
    15301 gaaaaagcta gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca
    15361 aaaaaaatcg gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa
    15421 ttgtcggaag ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat
    15481 cgcgtaccgg aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa
    15541 tggcaaaaag ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat
    15601 attgaattaa caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat
    15661 gttttgctga atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat
    15721 cagcttaaag aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct
    15781 gaaacagact ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa
    15841 gtaatcgaac aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca
    15901 tttatgactg gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat
    15961 ttcaaaatgg ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac
    16021 atcgatgata aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa
    16081 tcacaatcgt tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa
    16141 tatcctttct ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt
    16201 tatcgttcat tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact
    16261 taccctctcc atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta
    16321 gatgttgctg aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga
    16381 ccaacaaatg atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt
    16441 tattttccct cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag
    16501 caagaaatag atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg
    16561 atgtcagagc atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat
    16621 gcaacacatg aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt
    16681 aatgtatatc aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta
    16741 tagatgtttt cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga
    16801 tcgctgatta tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata
    16861 ccaaaagagt tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct
    16921 ttgtgacgct tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg
    16981 agaacgaggc aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac
    17041 agatctcgat gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag
    17101 cggccgaact atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag
    17161 gagcaatcgg cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg
    17221 atcctgaact gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag
    17281 tgatcaatca tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg
    17341 gatatgaaga agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc
    17401 tggaatggtc tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa
    17461 aatgatggag gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa
    17521 aagttacttc tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc
    17581 acaaggaaca atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc
    17641 ggaattggca ggagaacacc gtttattcgg aagaatagga ctgtgacaag acttttgtca
    17701 cagtcctttt ttcgaattca acaagtaaaa cttttaagaa atattcagag agtaaaatta
    17761 aaaattgctg ataaacgaag atggaaattt tatgtatttt tattggtcta tgcaataaat
    17821 aaattaaata ttcagaatac aattgatttt aacgaaaggg cttctatcaa tggttgtgtt
    17881 gtatttatct gcccaggtag agtaaaatag atcattatct aaaaggtaat tgttcatact
    17941 atataagtca tttgaaagta aaaagatact gtaaataaaa aatgtggtta aataattctc
    18001 ataatcagtg taagcgttat caaaaatata gcatacttgc tttgtagatg aatgacacga
    18061 tatatgtttg gtatgttctg caagacagat gaatatggag gtggagacaa tgtcttccaa
    18121 agaactaaca atgtatttag agaaagaatt ggatcaactg aaagaaaagg ggttatacaa
    18181 tacgatcgac atactagaaa gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat
    18241 gatcaatcta gcatccaaca attatcttgg atttgccaat cgcgaagaat tgaaaaaagc
    18301 atgtattgaa gcaaccgaaa cttatggtgt aggagcagga gcagtccgaa cgatcaatgg
    18361 ttcgctgaaa atccatcagc aattagaaga aaaaatcgca gaattcaaag ggacagaagc
    18421 agcaatcgcc tttcaatctg gattcaactg caatatgggc gctatctctg cagtaatgac
    18481 aaaagaagat gctatcttat cggatgaatt aaaccatgca tctattattg acggttgccg
    18541 cttatccgga gcaaaaatca tccgaattaa acatcaagat atgaaagacc ttgagaaaaa
    18601 agcaaaggaa gcgatagaaa gtaagaaata taaaaaaatc atgtatatca cagatggtgt
    18661 tttctccatg gatggagaca ttgcacgatt gccggaaatt attccgattg ttgaaaaata
    18721 tggactgatc acctacgtcg atgatgcgca cggctcaggc gtaactggaa aaggtgcagg
    18781 aaccgtcaag cattttggat taagcgacaa aatcgatatg caaatgggca cgttatcgaa
    18841 agctataggt gttgttgggg gttatgtagc tggatcgaaa acactaatcg actggttgaa
    18901 agctagaagt cggccattcc tattttctac ttcgctaact ccaggtgcag ctgcagctgc
    18961 attggcttcg attactttga tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa
    19021 tgctaattat tttaaagaag aattgaaaaa agtaggctat aacatcggta tgtctgaaac
    19081 acctattact ccagtcattt tgggagacga aaaagtgact cagactttct ctaagaagct
    19141 gatcgaacat gggatttatg ctaaaccaat cgtttatcca actgtgcctc ttgggacagg
    19201 caggatcaga aatatgccga ctgcggaaca tacgagggaa atgcttgatg aagcagtagc
    19261 agtttatcaa aaaataggaa aagaaatgga aattatttaa ctaaatttag ttttttcgtc
    19321 tatttaaaat tagagaacat gttcacgcat atattgttaa tcatctgaag atagtagtaa
    19381 aaaagatcag ggaggatcat catgaaaaaa gtattggtga caggatgttt aggacaaatt
    19441 ggttcagagt tgatcacacg gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca
    19501 gatatacgca tgcctgaagt cattgagaaa aacgatatct ttgagatcct agatgtaatg
    19561 gattacgaaa aaatgcgcag catagcagaa caatatcaag tggacacatt gattcatttg
    19621 gctgctcttt tgtcagctgt tgcagaagca aaaccacaat tagcctggaa tttgaacgtg
    19681 actgggctgg tcaatgcatt ggaagtcgct agagaattcc agctgaaatt ttttacgcca
    19741 agttcaatcg gcgcgtttgg tccaaatact ccaaaagatc ataccccaca agatactctt
    19801 cagcgtccta caaccatgta tggggtaacg aaggtagctg gagaactact atgtgattat
    19861 tattatgtga aatatggcgt agatactcgt ggtgtccgtt tccctggatt gatttcttat
    19921 gcaaccttgc caggaggcgg tacgacagac tacgcagtcg atatttatta ttcagcaatc
    19981 aaagaaggaa agtacactag tttcattgat caaggtacat caatggatat gatgtatatg
    20041 ccagatgcga tcgatgccat tataaaactc atgaatgcag atcctgatcg attggttcat
    20101 cgaaatgcct tcaatatatc ggcgatgtct tttgacccgg aagagatcaa agcatctatt
    20161 caaaaaatca taccagattt tgaaatggat tatgatgtgg atcctgtacg tcagtcaata
    20221 gcagaatctt ggccaaattc actagatgtt tcttgtgcgc aagaggaatg gggcttttcg
    20281 ccgaaatacg acttagatgt gatgacaaag gatatgttgg aaaaactgaa agaaaaattg
    20341 gtagagacgg tataaaacaa gaaccacatc caaaaagttt tttatacttt tgggattggc
    20401 tatggtatga aaggggacaa gggatggaag aatatcaagc cacgccagta aaagaagtaa
    20461 aaatagaaaa tgaattaaaa cgtacgatgg gattttttac tgctttatcg actgttatgg
    20521 gcacagtgat cggtgcaggg gtatttttta aagccgcaag cgtagcagaa gttacaggat
    20581 ccgccagttt gcatatgttt tcttggtttc tcggtggtat gatttctgtc tgtgcaggac
    20641 taacaggagc tgaactggca gctgctattc cagaaacagg agggatgatt aaatatatcg
    20701 aacggattta cggaaataca gcagcttttt tattgggctg ggcgcaagtc gtgatttatt
    20761 ttccagcaaa tgtagcagcc ttgtcgatca ttttcggtac acagttcgtc aatctatttg
    20821 gtctttccca gtcaatgatt gttcccgtag ctgttacagc tgctgtatcg atcctgttga
    20881 tcaatttttt aggttctaaa gctggagggg cttttcagtc gattacgctt gtctgcaagc
    20941 ttattccttt gtttgtgatc gtcattttcg gtttgttccg acaagaagga gtagacttcc
    21001 aactgtttcc gatccaagca ggagaaaatc tttcattttt ctcagccttg ggagcgggct
    21061 tgctagcaac gatgttcgca tacgacggtt ggatacacgt aggaaatatt tcgggagaat
    21121 tgaaaaagcc ggcgaaagat ttgccaaaag caatttcttt aggaatcatc ggaatcatga
    21181 tcgtttattt attagtaaat gcggtgttct taagaaccgc atctatcgac ggagtcgcag
    21241 gaaacagcaa tgcggcaagt gacgttgcaa aaatgatttt tggcggcttc ggtggaagac
    21301 tggtcacagt tggtatcttg atttctgttt atggaaccat caatggctat acccttactg
    21361 gaatgcgtct cccttatgtt atggcaaaag aaaacaacct gccttttagc aagctctttg
    21421 ctaaacttca tgataaaact aaagttcctg tagccgcagg tatcttagag ctagtcatcg
    21481 ctattggcat gatgatgatc ggtggttttg atacactaac ggatatgctg atctttgtca
    21541 tttggatttt ttatacgatg gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg
    21601 atttgtttcg accatacaaa gttccaatgt atccgtttat tccactagta gcaatcattg
    21661 gagggacttt tatcgtatca agcacgctta tcactcagac ctttttagct tcgatgggga
    21721 tagtattgac acttgctggt atcccgattt atctctattt aaaacgtaga tagctaaaac
    21781 attcgaattt tattagaact gcacttctct gccaaacaaa atattttatt taaatgagga
    21841 gtacgtggaa gaaatgtcaa aagtactcgt taattttgat cacgtaaatg tcgttttgtc
    21901 tacgatgaaa tagcaaagta aaagacagaa atcgggctca accgatttct gtcttttact
    21961 ttgatttgcg taactgaact attcggcgta cgccgcgata aatatttaat aaaaccaagc
    22021 aaccagaaat gaaaatggga agccaaatca gatagacagg gtagccaagc actttagaca
    22081 aagcatgtgg agtcatttta tccatttcaa gaagtgcttg ctgccaagtc tgaagattga
    22141 agactagcat gaaacccgaa ataatcagga gaataaatcc taaataatga attgtagttc
    22201 tttttcttac ataagcgatc gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa
    22261 caaagtacat attcagcatc tcgacacccc ctttgtgttt atcttacttt actttgaaag
    22321 aaatgagaaa taatagcctc gttacttgct tgaaacagca aaggactgct aagataagag
    22381 agatacaaag aatcaaggag gagtcatatg gcaagttata tcgaaccgat tcgttttgcg
    22441 ctgattgttt ttccattttt agccttagcg atctcatctg ttttttttat ttatgaatat
    22501 cgaaagtatg gaacatttct agtaacaagg gcagttattt tgtattcttt cgttttttat
    22561 ttattgtgtg cgtatttttt ggtgatctta ccacttccgg caaaggagac ggttgcccaa
    22621 atgacagggc caaagatgga gctgcattta tttgcttcat ggcaacattt cagagacaac
    22681 acggtattgg tcttgtctga acctagtacc tacctgccgg caatgaagca gagtgtattt
    22741 ttggaaccag cttttaatgt tttattactc ataccatttg gcatttattt aagatattat
    22801 ttccggcttt cttttatgaa aacgatgatt gctagtttct gcctttctct attttttgaa
    22861 ttgacgcagc taagtggact ttattttatc tacccaagac cataccgctt atttgatgtg
    22921 aatgatttag tgacaaatac attgggtgga atcattggtt ttgtcgttac gccgttattt
    22981 acatttatgc ttcctacaag aagacgaatg gatgaacttt cttatgaaaa aggaaagact
    23041 gtctcattga ctcgacgctt ggtcgcttat ttgatcgact ggacattttt atctcttatt
    23101 aatttggtag cggcgatttt ctttaaaatt gcgttcgatt caggggcagt tacttctcat
    23161 atctggtggc ttttattaga agtaggggta tactttatga ttgtaccgtt tataaccaat
    23221 ggatggacac ttggcaaaaa aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga
    23281 atttctttaa aagcattgat gatccgctac gggtatctgt actttctttt ttatggttta
    23341 agctggttgg ggcagttgct tcagcaaatc atgaattcga caacgaatac ggtgttgcag
    23401 atgagtgcag tattcatttc tctcggtgtc ttgttactac aaggtatttt tattttaaat
    23461 atcttgctat catttttgag aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca
    23521 tatactgtta gcacgattgg ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa
    23581 aacgctttgt ctgtgttata atgccaaagt cgtgataatt taaagagaag aggtgcacga
    23641 atgaataatt ttgatatatt ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag
    23701 ggcttcaaac agcccacaga ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa
    23761 aatatcattg ggcaatctca aactggtaca ggaaaaacac atacgttttt gttgcctttg
    23821 tttgaaaaaa tcaatgcaga aaaagaggaa gtacaagttg ttattacagc tccaagtagg
    23881 gaactagctg cacagatcta tgaagcagct acacaaatcg ctaaattttc tacaccggag
    23941 atccgtgtct cgaattttgt cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat
    24001 caccaacagc cacaggttgt agtaggcacg cctggtagaa tattagacat gatgaatgaa
    24061 caagcgctga agattcacac cgcatttgca tttgttgtcg atgaagcgga tatgacattg
    24121 gatatgggtt ttttaaatga agtagaccaa attgcaagtc gattaccaga aaagctgcag
    24181 tttttagttt tttctgccac tattccagaa aaacttcgtc cattcttacg aaaatacatg
    24241 gaaaatccaa tcattgaaga aatcaaacca aaagcagtta tttctgaaga cattgaaaat
    24301 tggctgattt caacaaaagg taaagatgag aatcaaatga tttatcaact gctgacaatt
    24361 ggtcatcctt atttagcaat cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc
    24421 gattacttga aaaaccaagg cttgaaagta gcaaagattc atggagacat caccccaaga
    24481 gagagaaaac gagtgatgcg acaagtacag aatctggagt atcaatatgt tgtagcgact
    24541 gatttagctg ctagagggat cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt
    24601 ccagaagatc tagacttctt tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa
    24661 ggaacggcca ttacgttata ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa
    24721 ttgggcgtct catttgaacc aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat
    24781 cgtaatagac gtgccaaacg agaaaaatca aaagatgtat tagatcccac gctgattggt
    24841 cttgtgaaga agaaaaagaa aaagatcaag cctggttata agaaaaaaat cgattgggca
    24901 attgcagaaa aaaataaaaa agaacgcaaa atcgaacgca gacagcaaag ccgtactgct
    24961 cgtaaaaata agaaaaattc acataaataa aaaacaagag gttgtgacaa aaggcgtgtc
    25021 acaatctctt gtttttaagc tagacttttt ttcattccga tacaaagtac ttcatcttca
    25081 ataaaaggtt ctgtgcaagt atgataccct tgtttttcat aaaaatctac agccgtcatt
    25141 tcagctacta cataactagt ctgacaacct tctgaaaccg cccgtttttc agcagcttca
    25201 agcaactgac cgcctaaccc ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc
    25261 ttgaagcaag taggtgtttt cttttcataa cgcaaagtag ctactggtct tttttgatca
    25321 agaaatagat aataggtttg ttgcgggata tcttttccgt cgaattctag ttctgcagga
    25381 atcccttgtt ctaatacaaa aacttgatat ctcaaaaagc agctagctgc gcgtatccat
    25441 tcatccgctc caactatttt tttcatcata ttcctccttg tcatgaatag cagagatgta
    25501 ttgattcctt tattcttatc gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa
    25561 taaaaattga gatattgaca gtgatcaata gaatgcgtat actaaataag tacgaaaagg
    25621 atatgatttg ttttttaaga ctcacagaaa attctttctt ggctgaaaaa agaatagtca
    25681 ctaatcaaat tgctcccttt ttctattttg gtagaaatac cgacgcagct cagcgttatc
    25741 gagtttaaga ggtaatgatt acacatcatt gcaatcaagg tggtaccgcg agttttcgtc
    25801 cttggctgtg atggtgtttt tttgtattta atttatagaa agcaggtaat gacatgaaac
    25861 aattaagtag tgcacaagtt cgacaaatgt ttcttgattt cttccaatct aaaggacata
    25921 cgattgaacc tagtgcgtct ttgatccccg tgaacgatcc aaccttattg tggatcaact
    25981 ctggtgttgc aacattgaaa aaatattttg atggttcagt tgtgccagaa aatccaagaa
    26041 tcacaaatgc ccaaaaatct attcgaacaa atgatatcga aaatgtcggt aaaacagcac
    26101 ggcaccatac gatgtttgag atgctgggta atttttcgat tggtgactat ttcaaaaatg
    26161 aagccatcca ctgggcatgg gaatttttga ctgatccaaa atggatggca ttcgatccag
    26221 aaaaacttta tgtaacagtt tatccaaaag atacagaagc aaaacgcatc tggcatgaag
    26281 aagttggcct gccactagat cacattattg atgtagaaga caacttctgg gatatcggtg
    26341 caggcccatg tggtccggat acggaaattt tctatgaccg tggagaagct tataacgatt
    26401 tagcagaaga cgatcctgaa aactatccag gaggagaaaa tgaacgttat ttagaaatct
    26461 ggaacttagt attttcagaa ttcaaccata agcctgataa cacatttgaa ccacttcctc
    26521 ataaaaacat tgatacgggg atgggattgg aacgcatggt ctcaatcgtt caagatgcgt
    26581 caacgaactt tgagacagac ttgtttatgc ctattatcca cgccgtagaa aagctaagca
    26641 aacaagtaaa atatggtgaa aacccagtaa cagatatttc ttacaaagta atcgcagatc
    26701 atattcgcgc cctttcattt gctattggcg acggtgcatt gccatcgaat gaagggagag
    26761 ggtatgtctt acgccgtttg cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg
    26821 aacaagcatt cttatatcgt ttagtccctg ttgttgggga aatcatggta agttattatc
    26881 ctgaagtact tcaacaaaaa gaatttattg aaaaagttat ccgtacagaa gaagaacgtt
    26941 tcaatgaaac aatcaatgaa ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa
    27001 acgaaaacgg gacgactcta gcaggaaaag acatcttcaa actatacgac acatatggct
    27061 tcccggtaga attaacggaa gaggtggcag aagatgaagg actgaaagtt gatcatgctg
    27121 gttttgaaaa agaaatggat gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa
    27181 aatcaatggg ggtacaatct gctttattga cagatatcaa agtagaaagt aaattcattg
    27241 gatacacaga acaagaatca gatagtcaat tattggtcat cgttaaaggt gagtctcttt
    27301 tgaatgaagt cgcagaaggt gaagctcaaa tgatctttga tcaaacacct ttctatgcag
    27361 aaatgggtgg gcaagtagcg gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag
    27421 cagttgttac agatgttcaa aaagcgccta atggtcaatt tatgcacacc gtagaagtaa
    27481 aagcaccaat gagtgaagga caaacctatc atctattgat cgatcaatcg atgcgcaatc
    27541 ggatcatcaa aaaccataca gcaacccatc tactgcacaa ggcattgaaa gaagtacttg
    27601 gcgagcatgc aaatcaagct ggttctttag tcgctcctgg acatttacga ttcgacttta
    27661 cccactttgg tcaaatcact tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa
    27721 aaatctggga agctatccca gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca
    27781 tgggtgcaat ggctttgttt ggtgaaaaat acggacatga cgttcgagta gtaaatattg
    27841 gcggctggtc aatcgaactg tgcggaggca cacatgtagc aaatacagaa aacatcggaa
    27901 tcttcaaaat cgtttctgaa tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa
    27961 caagtaaaga agcgtacgaa ctacttgaag aagaagaaaa acaactaaaa caagtagcaa
    28021 caattgttaa atcacctcaa ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc
    28081 aagtaagaga gctacaaaag gaaaatgaac aattagctag caagttagcg aatcaacaag
    28141 ctggtgatgt attcaaagat ccagaaacga tcaatggaaa aacaatcatt gcagcacaag
    28201 taaaagtgaa agatatgaat cagctgcgtc aattagctga ccaatggaaa caaaaaaata
    28261 tttcagatat tcttgtttta gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa
    28321 tgacgaaaga atcaaatgaa gctggactaa aagctggcga cttgattaaa gcaatcgcac
    28381 cgaaagtagg cggtggcggt ggtggacgtc cagacatggc acaagctggc gggaaaaatc
    28441 ctgctggtat tccagatgca cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg
    28501 tttacgtttg acagaaaaac aaaagttcaa acattgaacg aaacatgatt atgggggcga
    28561 agacaaacaa gtcttgccct catacttttt tcaattggat ggcgcatcta atcaaatggt
    28621 cggattcgga tggtctttag ttgacgaaag tgacagaaat ttttacgatt agtataaata
    28681 aaaaggggtg aaaaaatgaa tagtagaaat aagacctttc gactagtctt acgtgcgatc
    28741 ttgttagcaa tcatcatcgt acaagcgatg gttccttggc tggggtttat tccattagga
    28801 ttcattagtt taacaattat ccatatcaca gtaattattg ctgcagtagt attaggccca
    28861 aaagatggca tggtgattgg cttgttttgg ggaatcgcta caattgttcg tgcctatgcc
    28921 atgccaacaa cgccatttga tacattagtg ttcacgaatc cgattatctc ggtagttccc
    28981 agagtacttg ttggcttagt agcaggcttg gttttccact ggatttatca aaaaaataaa
    29041 tcaataacga tcagctcagc ctttgcaggg gtactgggtt cattggtcaa tacagtctta
    29101 gtgttaggat tcatgggatt attttataca ggtgcaacgg cagatgccta tggtgtagac
    29161 tctgctcttt tatttaaaac cttagcaggg gttgcagcaa tcaatgggat accagaagca
    29221 ataggtgcgg gaatcataac accattacta gcaaaagcgt tatttgcagc aacaccgttg
    29281 aaaccagaat aatgaccatc ttttaatagt aataggacgc acgatagata gaaaaaggaa
    29341 gagaggagag gaaaacaatc gtcctctcca tctttttacc tgagcaacaa gcaaactaga
    29401 aggggtaaaa aacttggcta agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg
    29461 gacaaaaaaa ggagtactag cgggtatctt aggattggtt acgaatctga tcttgtttgt
    29521 ctctaagcta atgatcgggc ttttttcagg aagtgtctcc attatggctg atgcagtgaa
    29581 cagtctatca gatactgctt catccatatt gactttgatc ggatttaaaa ttgctgcaaa
    29641 gccagcagat caggaacatc cttatggaca cgaacgcttc gaatatatca gtgggttatt
    29701 tgtttcgatc atcattactt atgtaggatt tcaattttta gagtcttctg ctgaaaaaat
    29761 catccatcca gaaaatgtct ctttaacacc catcgtcttt tttgtcctaa tcatttccat
    29821 ctttttgaaa tttgcacaag gcaaaatgta tcaggttatt gcgcaatcca ttgattctga
    29881 aacattgaga gctacagcaa cagatagtta taatgatgtc tttactacac tggctgtatt
    29941 gctttcagca gcaatcgaat gggtaactgg atggcgtatt gatggatata taggttttgc
    30001 tttagcatta tatatccttt atagtggatt gacgatgctt cgaaattttg tatacgaact
    30061 attaggaagt cgcccaacgc cagcagagat tgaagaaatg gaagagcatt taaatgcttg
    30121 cgatgatata cttggctatc atgatttatt ggtgcataac tatggaccca ataaacgctt
    30181 tgcatcagtc cacatcgaag tagatgaatc aatgaatttg aatgtggctc ataagattat
    30241 tgatctgata gaaaaagact ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga
    30301 tccaatgcct gttgataacg aaaaatacta taatatcttg cggcagttaa agcaaattct
    30361 tcttgaaaca ggtacagggc ttaatattca tgattttaga gtaattcatc aaggaaaaac
    30421 gctgcaattt gatgtagtgg tccctgagaa attccagttt gttgatacag aactagaaag
    30481 aatcttacgt agtcaaatag aagaaaaaat cggagattac caattggaaa ttacgtttga
    30541 tcacaattat cttttatatt aaatgagaaa ctataaaaag actccttaaa gtttttaatt
    30601 taaagagtct ttttttcttt taattgttta tggagagcct gacgtgcaag attatctgct
    30661 cctttatttt tgttttcggg caaccattgc aggatcaata aagaaaattc ggatagaagt
    30721 tcttggattc tttgaaggta gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc
    30781 gtagaaacta gtgttttgct gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt
    30841 ttctttagaa attctaaagt ttttaaaaag acttcgaact ctgcttgatg gtttgtccct
    30901 atagaaagcg ggatagatag ctgttcttgt ttaccttgat agacaatcaa gattccacct
    30961 ccactaggac cagggttgcc ttttgtagag gcatcaatgt atacttttaa cataaaaaga
    31021 gattctcctt tttgttcaaa ctaatgttat ggtaagatta tatcattact gaatcggtga
    31081 gaggttagag aatgaaaaaa aatattcgat ggcagcctga actggcacat acgatcatct
    31141 attggtcatt gaccttgatc attctgtttt ttagtctgat tctttcttta gaaaacacag
    31201 gtccttattg gaaaagcaat tttgttttag gtatttttct tgtttttcta tttctaggtt
    31261 tgcggcgttc ttttatttta aaatcaaact ctgtgattat acgttatgct gctttttgga
    31321 aagaccgaga aatatctgtc ggacagattc gatctttgac gccaactaat actggggtgc
    31381 aaatgatgtt gcaagatgag aaagccccat ctgttttcat gatgcgaaaa aaagatcaag
    31441 tagtcctttt tgatcatttt gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt
    31501 atacgaaaaa aagaaaaagc taattttaaa aatcagaacc taaaaaaacg aatttttaca
    31561 ggaaaagaaa agagtgagac gtacggttca tcccgtttag cgtctcactc tatttgttta
    31621 tctattcatg catactagta tcggtagtct gaacgttgcc tttcttgaca tgggcagctt
    31681 gtgttcctcg attcccttcg atgatctcaa attcgaccac ttgattttct tccaaagttt
    31741 tgaatccctc ttcttcaatc gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac
    31801 tgataaagcc gtatcctttt ttgttgtcaa accattttac aattcctgtg gtcaaagcac
    31861 tcacgacctt tctaaagtct gatcatagcc ttcattataa agagtattta taagtacgtc
    31921 aaataaatta aaaacctgta attttatgtt ttttttctga ataaaaaaag gtataattgg
    31981 catatcttaa caaagaagat attttgttga ataaagttcg tgaaaagggg taatcaaatg
    32041 aagaatgatc tgcaaatttc acaagaaaca gagctagaac cgattgttga agtggctagt
    32101 aaattagatc ttaaggaaga cgatttggaa ctgtatggga aatataaggc gaaaatcaat
    32161 ttttctggaa tcaatcgatc aaaggataac gctgaggggc atttgatctt agttacttcc
    32221 atcaatccaa caccagctgg cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg
    32281 ctcaataaaa tcaataaaaa atctgttatt gcattgcgag aaccctcact tggtcctgtg
    32341 atgggaatca aaggtggtgc tgcaggcgga ggatatgcac aggtattgcc tatggaagat
    32401 atcaacttgc attttactgg tgacatgcat gctatcacaa cagctaataa tgcattatct
    32461 gctcttttag ataatcatat tcatcaagga aatgaattga acatcgatgc ccgccgagtg
    32521 atttggaaac gggtagtcga tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg
    32581 ggtgggccaa tccaaggtgt tccaagagaa gatgggtttg atatcacagt tgccagcgaa
    32641 atcatggcta tcttgtgtct tgcggcagac cttgaagatt taaaggctcg tcttgcaagg
    32701 atcgttgtcg gctatacatt tgaccgtcag cctgtgactg ctggagactt gaaagcagaa
    32761 ggcgctctgg cattactgct gaaagatgca atcaagccga atcttgtaca aacaatttac
    32821 ggaacaccag cttttgtcca cgggggaccg tttgccaata tcgctcatgg ctgcaatagt
    32881 attttagcta ccaagacagc attgagatta gcagattacg ttgtcacaga ggcaggattt
    32941 ggtgcagatc ttggcggaga gaaattttta gatatcaaag tacctaactt gaaaaaagcg
    33001 ccggatgctg tggtgattgt cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa
    33061 aaagatgaac ttaaaaatga gaatcttgat gctttgaaag taggttttgc aaatttaaag
    33121 cgccatatac gtaatatgga acaatatcag ctacctgtga tcgtcgcaat caatgaattc
    33181 gtaacagata cagacaatga actcacactg ttggagcact tgtgtgaaaa tcaggggatt
    33241 cttgccaaga gagcgagtgt atgggcagat ggagcagaag gcggcgttga tttagcaaaa
    33301 gctgtagtac gattgattga tagaaaagaa gctaattaca agccattata ccgcctagaa
    33361 gaaacgatac aagaaaagac agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt
    33421 gttttcagca aaaaagcgca aaaacaaata gaagagttta cgaagaatgg atgggacaaa
    33481 ctgccgattt gtatggcaaa aacacagtat tcattttctg atgatccttc actgctaggt
    33541 gcaccggaag atttcaccat cacgatccgc gaaatcatcc caaaattagg ggcaggattt
    33601 ttagtagcct tgactggaga tgttatgacg atgcctggtt tgcctaaaaa acctgctgca
    33661 ttgaatatgg acgtgacaaa tgacggagaa gtgttgggat tgttttaagc ttgattataa
    33721 tctaccatcg aaggagctgt attttgacta cagcttcttt ttttagacga tagggaatta
    33781 attttgttta cactaggatc attctttttc tttattgtat tttgagctat actaaagcat
    33841 aataggaggg tatgatgaaa acgacattta taagaaaaac cggtttttac ggtatgggta
    33901 gctcgattca gctaagaatc aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga
    33961 taacgcttga tctggaagtg ccctttacga tgcaggtcac cttttattgg ctaaaaagcc
    34021 ccgtttatac agtgaaagaa gcaaagcctg cttatgtgat cacaatgaat tacttgattc
    34081 ttcaaatcta tccgttttta tttctagcaa ctgggttggg ggccgttttc ctacaaaatt
    34141 tatttttcag tttttttgca gttattgtgt tgattgcgtt ctttttctat ataaaaaacc
    34201 gggcatattt gatcaaggag gaaaattatg gcaaactctg atcttttttt aatcagtttc
    34261 aatcgaatcg aaaaatggat gagagacgag atgggaaatg cacgaaatat gggatttacc
    34321 gaacttgtca gacgcctagc tcaaagaaaa caattaatga tcagaaaata cgaagatgat
    34381 cttttacagc ttgctcagtt aaggaatgcg atcgtccatg atcggatcgc agtcgatttt
    34441 gtcattgcag agccaaatga ttggacagtc aagcgaatcc agctgatcga acaagaactg
    34501 attcagcctg aaaccgtgct gcctcgattt gcaaaacgtg taacaggttt tgaacaagat
    34561 cttccgttac ttgaactgtt aaagatagta gcagaaaaaa ggtattcaca atttccgtta
    34621 tacaataaag gaaaatttgt agcattgatt actttacgta atatcgggtt ttggctagca
    34681 aaagaaagcc aaaaaggtcc ggttgattta acaaataaac gagcgttgga tttgatcatc
    34741 caaaacggaa agtacacaaa ttatcatttt gttccagcta aaacacatat ttacgaggtt
    34801 gaagcgatgt ttcgtgagca agggactttg gaagccgtac ttatcactaa agatgggaat
    34861 cccgatggta atctgctcgg gatcgtacga cctcgtgata tttataaaca aatagaaaag
    34921 gattgatttt ttgttaatta tttattggat attcagcgct ttgctagtag gagtggatca
    34981 gttggtcaaa tggtggatca ctgataatct agcgcttgga gagacgaaaa atcttatacc
    35041 aggtattttg tcactgaatc atattcgaaa cactggagcc gcatggagta tgctagaagg
    35101 gaagatgtgg ttctttacta tagtgacact cattgcagtc gttgtgattc tcacattgat
    35161 gattaaaaat cgtgaaaaag gtaatcgctg gttcatgatt ggattgagtt tgattctagc
    35221 tggtgcgatt ggcaatttta tcgaccgtat tcgtcttggc tatgtagtag atatgttcca
    35281 gacagatttt atgagctttc cgatttttaa cgtagcagat gtgacacttg tatgtggagt
    35341 gatttgtatg ttaatttata ttattttaga tgaaaaggat caaagaaaaa aatgacagaa
    35401 cttaaaacga cgattcacca tgaatctggt aggatcgata aagtattgac taatttattc
    35461 accgattaca gtcgttcgca aattcagctg tggctgaaaa atggagcggt ttctgtcaat
    35521 ggacagccag tcaaagcaaa ttacaaagta aaacaaggag acgagattac tgtcttgatc
    35581 cccgaaccag aaacattaga tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa
    35641 gatgaagatg tagcggtgat aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt
    35701 catacgcatg gcacaatggt caatgcgctg atgtaccata tcaaggatct ttctacgatc
    35761 aacggcgtta ttcgtccagg tattgttcac cgaatcgaca aagatacttc cggactgcta
    35821 atggttgcca aaaatgacca ggcacacgaa tcattagctc agcaattaaa agataagacc
    35881 tctttgcgca aatatgttgc tttagtccat ggagagattc ctcacgaaaa aggaaccatc
    35941 aatgcgccaa tcggccgttc gaaggaaaac cggaaaatgc aggcgataag agaagatggg
    36001 aagtcggctg tcacacattt tactgtattg gaacgtttcg aaggatttac tctggttgaa
    36061 ttaaaactag aaaccggaag aacacaccag atccgtgtgc atatgaagta tatcggttat
    36121 ccgttagctg gagatccagt atacggaccg aagaaaactt tgaaaggcaa tggtcagttt
    36181 ctccatgcgc aaatgttagg ttttacgcac ccgactacaa acgaaaagat gatctttgaa
    36241 gcacctcttc cagaagtttt tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt
    36301 taatttagat aacggtatag taattaacaa cgaaagcata aaacttaata gaattccttt
    36361 aagtttagtc ccgtgaggct aagaagggtg ggagcaagga tacgtatggt gcgccataag
    36421 tagattgctc attgacatag tatagctatc agtgtatcag tatacgctca gtctaaacat
    36481 tcctcctgtc ctcttagggc aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg
    36541 caagcaaaag aagtagtaga tcaggtaacc atgaagcgag cattgactcg aattacttat
    36601 gagattatcg agagaaacca tagtatccaa gatatcgtat tggtaggtat caaaacacga
    36661 ggaatctaca ttgcatcaag aattgccgaa cgcctgaagc agctagaaga tatcgatatt
    36721 cctgtaggag aactggatat cactttatac cgagatgaca aaaaagaaaa tccagaagaa
    36781 ccggaacttc attcttctga catcccagtt tccttagaag gaaaagaagt tattttgatt
    36841 gatgatgttt tgtatacagg acgaacgatt cgagcagcaa tggacgcagt aatggatttt
    36901 ggcagaccaa gaaaaatttc attagcagtc ctggtcgatc gtgggcatcg agaattgcca
    36961 atccgtgcag attatgttgg gaagaacatt ccaacatctc gggcggaaga gattttagta
    37021 gagatgcaag agcttgatgg acaagatcgc atcatgattt tgaaagagga agattagagg
    37081 agagagaaac atgtcggaga aaaaaggatt tcataatgat gacgtggtgt tagatatcca
    37141 cgatcgccca accccaggac attgggtagg attaagttta caacacttgt tcacgatgtt
    37201 cggagcaacc gtgttagtcc ccatcttagt aggtatcgat ccaggtattg cgctagtcag
    37261 ttcaggacta ggtacattgg tgtacttatt tactactaaa ggaaagatac ctgcgtactt
    37321 aggaagcagc ttcgctttca ttgcagcgat gcagcttttg atgaaaacag atggttatcc
    37381 agcgattgca caaggagcag tcacaactgg attagtctat ttgattgttg cgctgatcat
    37441 caaaaagatc ggttcagcat ggctggataa aatcttgcct ccaatcgtcg tcggtccagt
    37501 catcatggtt atcgggttgg gacttgcggc aaatgcagca acaaatgcta tgtataatca
    37561 gggcacgtat gatttcaagt atattgctgt agcgctgatc acattaggat taacaatttt
    37621 cttcaacatg tggctaagag gttttctcgg attgattccg attttactag gtattattag
    37681 cggatacatc gtagccttgc tgtttggaat cgtggatact aagccaatcg tagacgctgc
    37741 ctggtttgct gttcctaact ttgagattcc ctttgtacaa tatgaaccaa agctatatct
    37801 tggagcaatc acaacaatgg cacccatcgc ttttgtgacg atgacagagc atatcggcca
    37861 tcttatggta ttgaataaac taactaaacg taatttcttt gaagaaccgg gtcttcataa
    37921 gacgttgaca ggtgatggat tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt
    37981 aacaagttac ggtgaaaaca ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt
    38041 gatcggtgga gctgcagtat ttgcagtcat tttaggattc gtaggaaaat taagcgcttt
    38101 gattttaagt atccctggac cagtcatctc aggaatcagt tttatcttat ttggagtaat
    38161 tgccgcaagt ggaatgaaga ttttaatcga gaacaaaatc gattttgata aaaagaaaaa
    38221 tctgttgatt gcttcagtta tcttagtagt aggaattggc ggactcgtct ttgaagtagg
    38281 cacatttaca ttgtcggcaa tggcattagc aactgtactt ggcatcattt taaatcttat
    38341 cttaccagaa acttcacgaa gcgaagaaaa ctaaggagga aacattatga tcatcaaatc
    38401 agaacgtatc agcttaaagc atttattgac cgttgaggca cttactgatc aagaagttat
    38461 gggcttgatc cgcagagggc aggaattcaa gcaaggtgct aattggactc cgcaaaagcg
    38521 ccagtacttt gctacaaacc tctttttcga aaatagtact cgcacacata aaagcttcga
    38581 tgtagcggag aaaaaactag ggctagaagt gattgaattt gaagcaagca caagctctgt
    38641 ccaaaaaggg gaaacactat acgatacagt actgacgatg tcagcattag gcgtagacgt
    38701 agcggttatc cgtcatggtg atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat
    38761 ccagtgttcg attatcaatg gaggagacgg aagcggacag catccaaccc aatgcttgct
    38821 tgatttgatg accatttatg aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt
    38881 aggtgatatc actcactctc gcgtagctaa atccaatatg cagatgttaa aacgtctagg
    38941 cgcacaagta ttcttctcag gtcctagaga atggtacgac gatgaattcg aagtgtacgg
    39001 acattatatg ccattggatg aactactaga tcaagttgat gtcatgatga tgcttcgggt
    39061 gcaacacgaa cgccatgatg gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca
    39121 tgggttaaca gttgagcgtg cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc
    39181 accagttaac cgtgatgtag aattagctga ttcattagtg gaaggattac agtcgcggat
    39241 cgttcagcaa atgagcaatg gagtattcgt cagaatggca atcttagaag cagtgctaaa
    39301 tggaaaagcg taattatagg aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat
    39361 caatacacga aaaaatatga cgacaccagc agaaatttgg atagaagatg ggcgaatcaa
    39421 agcaatcggt acaggattct cagaagccga atttgatgaa gtgtttgatg caaagggaca
    39481 attgatcaca cctggtttag tggacgtcca tgttcatttg agagagccag gatttacata
    39541 caaggagacg atcgaagctg ggactcgctc agctgctcgt ggcggtttca ctactgtatg
    39601 cgcgatgccg aatttaaatc ctgtacctga tacggcagaa aagctgagac aagtatatga
    39661 catcattaga aaagatgcag ttgtgaaggt attgcagtat gcgccaatca cagaaaatct
    39721 gcgaagtgag aaattagttg atcaagaagc actgattgaa gaaggtgctt ttgctttcac
    39781 aaatgacggt gtaggtgtcc aaacagctgg gacgatgtat ctggctatga aagaagctgc
    39841 aaaaaacaat aaagccctag tagcccatac agaagatgaa tctttattat ttggcggcgt
    39901 tatgcacgct ggcaagaagg cagaagaact tggtcttccc gggatcttaa gcgtaaccga
    39961 gtcttctcaa attgctagag atctgttgct agctgaagca actggtgttc attaccatgt
    40021 ttgccatgtt tctactaaag aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat
    40081 ccacgtaaca gcagaagttt caccccatca tcttattttg atcgacgaag atattcccga
    40141 agatttcggc ttttggaaaa tgaacccgcc gctaagagga cgggaggaca gagaagcttt
    40201 gatcgaaggc ttgcttgacg gtacgatcga ttgcatcgct acagaccatg ccccccatgg
    40261 attagaagaa aaaagccaaa gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac
    40321 agcgtttcaa ctgatctata ctcattttgt agaaactggt cgattcacgt tggaacaagt
    40381 gatcaattgg ttggcagtta aaccggctga gatttttggt ttgaatgcag gaacgctaac
    40441 ggttggtgcc cccgctgatg tagcagtttt tgatattaca caaacttgca cgatcgataa
    40501 agaagacttt ttatcaaaag gggagaatac accttttatc ggctggaaag taaaaggcga
    40561 aacacaaatg acttttgtca acggaaaact tgtgtggcag aagggagaat aaaagtatgg
    40621 aacgtttgct tatattagaa gatggaacag ttttcgaagg aaaggctttt ggtgcagatg
    40681 ctagtgtctt cggagaaatc gtttttacaa caagtatgac tggttatcaa gaaactatca
    40741 ctgatcaaag ttttaacggt caaatcatta catttaccta tcctatggtg ggtaattatg
    40801 gggtaaatag agatgattat gaatcgattt ctccaacatg caaaggagta gtagtaaaag
    40861 aacatgcacg tgtagcttca aactggcgaa accaaatgac gctagattca tttttgaaac
    40921 aaaaaggaat tcctggaata tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg
    40981 aagtcggaac gatgaaagct agtatagttg atgctggaga ttccttcgaa catgcatttg
    41041 atcagctgaa agcaaccgtt ctagcaacca atcaagtgca gcaagtctca actaacaaac
    41101 cttatccaag tccaggaaca ggtcggaaag tcattgtcat tgacttcggc ttgaaacata
    41161 gtattttacg agaattgagt aaaagaaact gtaacctcac agtacttccc tataacacag
    41221 atgccgagac gattttagcg ctatcaccag acggagtcat gctgacgaat ggacctggtg
    41281 atccaaaaga tgtgccagaa gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga
    41341 tttttggtat ttgtctcggt caccagttat ttgctttagc caacggtgca gatacctata
    41401 aaatgaaatt cggacatcga ggattaaatc acccggtacg agaagtagcg accggaagaa
    41461 ttgattttac ttctcaaaat catggctatg cagtcgatga agccacgatt gatccagaaa
    41521 aactcatcgt aactcatgta gaagtaaatg acggaacagt agaaggagtc aaacacagag
    41581 attatcccgc tttcagtgtg cagtttcatc cagatgctgc ccctggtccg catgatgcac
    41641 tacatttatt tgatgaattt atggaaatga tggacgcagg aaaggagcag taaacagatg
    41701 ccgaaacgta cagatatcaa gaaaattatg gtgattggat ctggtccgat catcattggt
    41761 caagccgcag aatttgacta cgcaggaaca caagcctgcc ttgccttaaa agaagaagga
    41821 tatgaagtgg ttttagtaaa ttctaatcct gcgacgatca tgacggataa agaagtggca
    41881 gatcaagtat atatcgaacc gatcacactt gaatttgtat caaggattct tcgaaaagag
    41941 cgtccagatg ctctccttcc aactttaggc ggacagacag gcctcaatat ggcaatggag
    42001 ctggcagcat caggtatttt agaagaattg aacatagaac ttctaggaac aaagctttct
    42061 gcgatcgatc aagcagaaga tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag
    42121 ccaatccccg agtctgaaat cgtcaataca gtagaagaag ctgtagattt tgcaaataaa
    42181 atcggctatc ctgtcatcgt tcgtccagcc tttactttag gcggaactgg tggaggtatg
    42241 tgtgaaaatg aagaagaatt acgtatcgta tcagaaaatg gattaagtct ttcccccgct
    42301 acccaatgtt tgattgaacg aagtattgca ggttttaaag aaattgaata tgaagtgatg
    42361 agagattcgg ctgataatgc aatcgttgtc tgcaatatgg aaaattttga cccggtgggt
    42421 atccatacag gtgattctat cgtatttgcc cctagtcaaa cattgtctga ccatgaatat
    42481 caaatgcttc gtgatgcttc tctttcgatt attcgagcac ttaagatcga gggaggctgt
    42541 aatgtccaac tcgctcttga tccacatagt tttaattatt atgtgattga agtcaatccg
    42601 cgtgtttctc gttcatctgc gttagcaagt aaggcgactg gctatccgat cgctaagcta
    42661 gctgctaaaa tagcgcttgg attaacatta gacgaaatga aaaatcctgt tacaggaacg
    42721 acttttgcag aatttgaacc tgcattagat tatgttgtag ctaaaattcc tcgctggccg
    42781 tttgataagt ttgaaaacgg agaacggctt ttaggcacgc agatgaaagc aactggtgaa
    42841 gtgatggcta tcggtcggaa tatcgaagag tctttattga aagctgtgcg ttctttagaa
    42901 atcggtacac accatatcga acttcctgag ttggcagaca tcacggacga tgacctaatc
    42961 caaaaagtgg ttcgagcaca agatgatcgc ctgttttatg tagctgaagc tattagaaga
    43021 gggtacacca tagaagaatt agcggatttg acaaaaatcg atttattctt tttagataaa
    43081 ctgttgcata tcgtagaaat cgaacagcag ttgaaacagc agccgttgga tgacaagata
    43141 ttgaaaacag cgaaacaaaa cggttttaca gacaaaaaag tggcggagct gtgggaaata
    43201 aacgaagaag ccgttcgcca gatgagaaaa gaaaatgatt tgattcctgt atataagatg
    43261 gttgacactt gtgcagcgga attcgaatca aaaacacctt atttttatag ttcctatgaa
    43321 tatgaaaatg aaagttcacg atcagaaaag ccctcaattc tagtactggg atctggtcct
    43381 atccgtatcg gacaaggggt tgaatttgac tatgcgactg tgcattctgt caaagcaatc
    43441 cagcaggcag gatacgaagc aatcatcatg aacagcaatc ctgaaacagt ttcgactgac
    43501 ttttctattt cggataaatt atattttgag ccattgacct tcgaagatgt gatgaatgtg
    43561 atcgagctag aacagccgat aggagtaatc gttcaatttg gtggtcagac agcaatcaac
    43621 ttagccgaac ctttgactgc tgctggtgta aaaatcttag ggacaacgat cgaagacttg
    43681 gatcgtgcgg aaaatcgtga tttattcgag caagcgctga aagagttgga tattcctcaa
    43741 cctttagggg atactgctac aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt
    43801 tatccagttc ttgttcgtcc aagctatgta ttaggcggac gtgcgatgga aatcgttgaa
    43861 aaccagcgag atttagaaga ttacatggaa catgctgtga aagcatctcc agaacatcca
    43921 gtactagtgg accgttactt gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt
    43981 gaaacagtac tcatcccagg gatcatggag catattgagc gtgctggtgt ccattcaggg
    44041 gattcaatgg ctgtgtatcc acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa
    44101 gattacacga tccgcttagc attaggtttg aattgtgtcg ggatgatgaa tatccagttt
    44161 gtcatccatg aaaatgaggt ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt
    44221 ccattcttaa gtaaggtgac aggaattcca atggcacaaa ttgcgacgaa agctattttg
    44281 ggagagaaac taaagacact tggttatgaa aacggacttt atccagaaac aaaagaagtt
    44341 catgttaaag cacctgtctt ttcttttacg aaattgcaga aagttgatac ttacttaggg
    44401 cctgaaatga aatcgactgg agaagtaatg ggatctgaca gacaattaga caaagctttg
    44461 tacaaggcat tcgaagcttc tggattacac ttgcctgaat atggagccgt attatttacg
    44521 attgcggacg aaacaaaaga agaagcacta gaattagcta aacgttttac agaaattggc
    44581 tatagtctga taggaacgaa acatacagct gcttatttcg aaaaaaatgg tctggtcgtt
    44641 gaaacagtag cgaaaatttc tgaggaagca gcagaaaaaa atgtagtcga cttaataaga
    44701 gatggtaaaa cacaagtggt tgtcaatacg attgataaag atcggggaaa tgcttctaaa
    44761 gacggtttca tcatccgaag agaagcagtt gaacatggta ttccattgtt tacttcttta
    44821 gacacagcag atgcgatcgt tcgagtgatg gaatcccgtg ctttttcaac taaccctatt
    44881 taacggcgag cacatgttaa gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga
    44941 aacaagaaat gatggaaatc gttcagcaaa aacagctggc accaaagatt tttgaaatga
    45001 cattgaaagg tgatctagta aaagaaatgc agcaaccagg acagttcttg catattctcg
    45061 tgccgcgtgc agacttgcta ttacgccgac cgattagctt gaatcatatc gaccacgaaa
    45121 caaatacttg ccgtatcatt tatcgggtgg aaggtgaggg gactagagtc ttctctactc
    45181 tttctgccgg agaccattta gatgtcatgg gtcctttagg caatggtttt gatttgtcca
    45241 atcttcatac aggagacgaa gtatttataa tcggtggagg aatcggtgtc cctcctctat
    45301 atgagttatc aaaacagtta gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg
    45361 ccacacatga agtcgtttat tatgaaaaag aatttaatcg tctaggagaa acaagaatcg
    45421 ctactgacga tggaacatac ggtatgcacg gaaatgtcgg taatttattg ttaggaacga
    45481 agacggaacc tacagctgtt tttgcatgtg gttcaaacgg gttattgaag acagtggaac
    45541 agctgttttc ttcgcatcaa aatgtccagt tatcattaga atcccgcatg gcatgtggga
    45601 taggggcttg ctatgcttgc gtttgtcata cgccggaaga tgaaacagga acaaaaagtg
    45661 tgaaagtttg tgacgaagga ccgattttta aaataggaga ggtaattata tgaatgagaa
    45721 tcctttagcg gtcaaacttc ctggcctaga attaaaaaat cctatcatgc cggcaagcgg
    45781 ctgtttcggt tttggcaaag aatacggaaa atactatgat ttgaatcagc ttggatcgat
    45841 catggtcaag gcaacgactc caaatgcccg ctttggcaat cccacgccaa gagtggcgga
    45901 aacaccatct ggcatgttga atgcaattgg tttgcaaaat cctggtctag acgttgttat
    45961 gcataaactt cttccagaat tagaggagta caatgagtta cccatcatcg caaatgttgc
    46021 tggtgcttgt gaagaagact atgtagaagt ttgtagcaaa attggcgaag caccaaatgt
    46081 gaaagcaatc gagctgaaca tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg
    46141 aacagattct gatgttgctt ttcaattaac acaagcagta aagaaagttt cttctgttcc
    46201 tgtctatgtc aaattgtccc caaatgtaac agacatcgtt ccaatcgctc aggcaatcga
    46261 agctggaggg gcagatgggt ttacgatgat caatactctt cttggtatgc ggattgacct
    46321 taagacaaga aaaccgattt tagccaatca aacaggtgga ctctccggac cagcaatcaa
    46381 gcctgtcgct attcgtttga ttcaccaagt agcaagtata tccagccttc caatcattgg
    46441 gatgggcggc gtgcagacag tggatgatgt tcttgagatg tttatggctg gagcaagtgc
    46501 tgtagctata gggaccgcga atttcaccga tccgtatatc tgtcctaaac taattaaaga
    46561 gctccctgtg cgaatgagcg aactaggcat cgaatcgctg gaaagattaa tcaaagaagt
    46621 gagagaggaa cgtgaacgat gaatcaacta gaccaacgac caattattgc attagatttt
    46681 tcaactaggc aagaggtaga agactttctt cgatttttcc cgaaagaaga aaaattgttt
    46741 gtgaaaatcg gaatggaatt gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa
    46801 gaggctggtc ataatgtatt tttagatctg aaacttcacg atattccgaa tacggtcgaa
    46861 aaagcaatgc ggggattggc aaagcttggt gttgatctca cctgtgttca tgccgctggc
    46921 ggtattcgta tgatggaggc ggcaatgcga ggattagaag aggggactcc agaaggtgga
    46981 aaacgtccgc tacttctagc tatcactcag cttacttcta caagtgaaga agaaatgcat
    47041 gccgatcaac taatcgaagt ttctttagaa aaaagtgtga tccattatgc tagctgtgcc
    47101 aaaaaagcag gattagatgg tgtggtttct tctgcttggg aagtagaagc gatcaaagaa
    47161 atggcaggag acgaatttgt ttgcttaact ccgggtataa gaccagaagg aacagtcgca
    47221 ggagatcaga caagagtagt cacaccttct caggcaaaaa agatcggctc aacttttatc
    47281 gttataggtc gaccaatcac tcagtcaact aatccttata aagcatatca aacgattcaa
    47341 acagaatgga gctaacctaa aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa
    47401 attgaagcag tatttttaaa tccaagtaat ccttttacat gggcaagtgg aatcaaaagc
    47461 ccaatttact gtgataaccg aatcacgatg agctatccaa aagtccgaaa agagatcgca
    47521 aaaggattag caagcaaaat caaagaagct taccctgaag tacaagtgat tgctggaact
    47581 gcaacagcag gaatccctca tgccgcatgg gtagcggaaa tactggattt gccaatggtc
    47641 tatattcgaa gtaaggcgaa agatcatggg aaaggtaacc aaatcgaagg acgtatattt
    47701 gaaggtcaga agatggttgt gatcgaggat ttgatttcta caggcggaag tgtcttggaa
    47761 gcagctgaag cagcaaaaag agaaggtgcg gatatccttg gtgttgcggc tatctttacg
    47821 tatgagttgc caaaaggaaa agcaaatttc gaaaaagccg aaatccctct tatgacgttg
    47881 accaattatt ctgtattgat tgaagcagct ttagaagatc gttatatcga tgagcaagaa
    47941 ttaactctgc taaaagaatg gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg
    48001 atgaagatag agaaatccga tttgtgtctc cagaatcatc aattggaata gcaaaaatcc
    48061 aaaaactgat tgcgagaagc atcttttcat caggtatgaa aagaaacttc tatcgtgatc
    48121 agtttttgat ttttttaaaa ttcatttttc taagactggt tagcagaaag aagagaataa
    48181 tctcttctct tttttctatt atatatagat tttctctatt ttttggtaag ggagctgttt
    48241 caaaaatggt aaagagcgga aatgttgcga atatcagtta tttaatgata tgatattcgg
    48301 aaaaagaaat ctcaaaggga ggtaactttt atggggttga atgaatttat tttaggattg
    48361 aacaatttag agacgatcac aagggcacct ggatttttta aatatacaga gcatacagtt
    48421 gcagcacatt cgtatcgtgt agcatctatt gcgcaagttc taggagacat tgaagaagcg
    48481 aatggacaac cgatcgattg gaaactgctc tatgaaaaat cattgaatca tgactacaca
    48541 gagcgtttta ttggtgatat caaaacgccg gtgaaatacg caagcaaaga gttaagaggg
    48601 atgctgcaaa cagttgaaga aaagatgacg gacgaattca tcaaccaaga gattccagct
    48661 gagttccaag aagtttatcg ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga
    48721 gaactattag ccatttcaga taaggttgac cttctttatg aatcttttga tgaaatcatc
    48781 aaaaataacc cggaaaaagt ttataaggag atgttcattg aggcagtgac tacgattaaa
    48841 agctttgaac atcggccttc tgttcagtat ttctttaaag agatatttcc ggaactattg
    48901 aaccaggaat tttatggaaa agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa
    48961 gaagaaaaat aaaaatgata tgagatgcag ggggaagtat agttgaaaaa gaatatggat
    49021 gtcgaatggg gctattctgg agacaaaggt ccggaaaatt gggcgagttt atgtgattgg
    49081 tttgcgagag gtgcggaatt tccattgcag tcgcctattc atttgaccaa gggggaagaa
    49141 accaaaatag aaggagacac gctttccttt cattatcaaa agcaacggtt tactgacaag
    49201 gaattcaaaa acacgattca tttagtacct tttgaccaac tcagttatgt agagttcaag
    49261 aaagaacgct attatttgac agatattcat ttccacttgc cttctgaaca tatcatcaac
    49321 ggaaaacagg aagatattga atttcatttt gttcatatga acagcaaaaa agagaatcta
    49381 gttgtgggtg tcatgtacca gctaatggta caggaaggtt ggctgtataa tcaagaaaat
    49441 ggggaaagtt ggaacttaga aaaacatgag cattgggtaa atccggatgt gttctttccg
    49501 gagcaaaaaa gccatttcca ttatattggc tcattgacaa ctccgcctac cagcgggcca
    49561 atccaatggt ttgtaatgga tcatgtagga aaactgaacc aagagtttct tctccccttc
    49621 gatggtcagt atgctcgacc gaacaaccga ccaatccagc ccttgaatgg aagagaaatt
    49681 tattatttcg aggagtttga gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag
    49741 caaacaatgg tagtttcaga gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt
    49801 cagctggtat caaagaatta gaaaccgagt tagggatcac gctgtttact cgaacgaatc
    49861 gaggggcata tttaacagaa gaagggcaag acttcttaaa tcgtgccgag aaaatattgg
    49921 tccagcttga tacattagaa actcactatc taacgagtga aaagagagac aatttttcga
    49981 tcgctgccca gcattatgat ttcttaggtc ctttgaccgc acagctactc gaggaattca
    50041 aagacgagat caaacaattt cggatcatgg agacgacaac agcgagagtg atcgaagaag
    50101 tcaaagagca gcacagtgag atcggcatta tttatatgaa tgagcataat caagctggta
    50161 tcagaagata tttacaacaa ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc
    50221 atattttttt aaggcaagga catccattag ctgaacacgc agagatcacc aaagaagaat
    50281 tgttatctta tcctcaagtt cgttttacac aggatggaaa taatttcccg tatttttatg
    50341 aagaccttat cgagatccct gatcaagaaa cagtgattta tacgagtgat cgaggtactt
    50401 tgatgaatat cgttcttgaa actgatgctt acgcttcagg atctggtatt gtgattggag
    50461 ggattcgcga gcacttgcgg ttaatccctt tggcagaagg cgaactaaat gaattttata
    50521 tcatttattc ggcgaaacgt ccgctcagtg aaatcgctca aagatttatc aaagagctta
    50581 ctcggttact ggaccagcag agctaagaaa agcagatgag aacgctgttt attgaacacc
    50641 gttgatcaaa aaagaggggt tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa
    50701 caatttataa ctaattgctg ttgtgggaaa gtacgcaaat atgattctag aaacactcat
    50761 tgattacctg ttttgtttca tagctgtaat tttttcttct tctggtgtca cgataatcgt
    50821 cttttggttt tcataaataa cataaccagg cttggcgcca tttggttttc ggatatgctt
    50881 tacctgtaca agatcaaccg gcacttgggc agagtatcgg tattttgaaa aataggcagc
    50941 taactcggca gcttctgtaa ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg
    51001 cgaaccgggt atattttttg catgaagcca atagtcggtt tttttagctg tcttcattgt
    51061 cagctggtca ttttgaagat tgtttttacc aacaagaatg agcgttccgt cagaagaaaa
    51121 gtattgatca ggctgagatg gtttcttacg tttttgtttt tttaagttct tctttttcag
    51181 gtatccttct gaagtcaatt cttctttaat tgcttcgata tccattgggc cggcaatttc
    51241 cagttgagat aagacagatt caagatattg gatttcatct ttggcttctt ggatttgttc
    51301 gccaatcagc ttaacagcgt tcttcagctt ttggtatcga tggaaatatt tctgtgcatt
    51361 ttgatttggt gtcaatgccg gatctagtgc tattttgatt ggccgatctt cttcataata
    51421 attaggtaaa acaacttctt ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag
    51481 ttcgccattt ctacggtaat tctcagcgtt ttcggattct ttgagtgttt gttcacgttt
    51541 tttcaattta tttttatttc gttttagttc attttcgatt tttcgaatca gttctccgcc
    51601 ttgttgtttt gcccgatctt tctctgcttt ttcgtgataa tagctatcta ataattgact
    51661 gagtgttgga taagctgtta cagcagatgc ctgttcggac aatacctgat aagcaatcgg
    51721 tgtgaaaaaa tctttatttt ccgtttcgta aaaagtagga ataggctgat ggctgatggc
    51781 tgagaaaaat tcgtcccata caaccatctt ttcattagga cgttccagta atcttttggt
    51841 cagctcttga gcgatatcga aaccgattcc ctgaaactga cgttggattg cttttggatc
    51901 tagttcagtt tgggaaagcc gctgaaagat tttttctttc tcggagctaa atgggttcaa
    51961 gacctcttgt tttggcggag caacatattc tacaccagga agcaaagaac gataagtgtt
    52021 ttgagagctt ccaatgtgtt tgatcgcatc cagtattttt ccagtttctc gattaactaa
    52081 gacgatcgta ctatgacgtc ccattaattc aacgatcaat atgatatttt gtagatcacc
    52141 caactcgttt cgtttagcaa agtggaaatg gatcactcga tcattttcga tttgttcgat
    52201 gtcttctaaa atagcgccat ccaaatattt tcgaagcatc atcacgaagt taggcggatt
    52261 atcaggattc tgataatcga tttgggtaat ctgtacacga gcataacttg ggtgcgcaga
    52321 taataataat cgatggtttt tgccgcgtga acgaatcacc agcacgattt cattttcata
    52381 aggctgatgg attttcgaga tccggccaga tagtaacgtt tcacgcaatt cgttgaccat
    52441 cgcgtgggta aatacaccat caaaggacat ggttcattcc tcatttcttt tcataagtat
    52501 attcattata acgaaaaaaa gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct
    52561 agaaaaatct tggactaatt acataaaaaa cagtgtttct atcgttgaaa tccaaatttt
    52621 taggtataat aaaaaatagg agaaaaagta gaaaagaagg tgtttgttat ttgggaagta
    52681 gttaaatcga gagtacaaga aatgaatgaa catttctcag tgtcattcgc agaaaatcca
    52741 agtaagtatt ttcgacgtct tgtttttctg ctcatcttat cattgctgtt aacagttgca
    52801 atgtttttat tgccggaaaa aatccgagaa gagcaagtaa atagtctcgt ttattcgtcc
    52861 ataccaacca gcaaattgaa accttttgat tacagcaaag ctgatcagaa aatcaaacaa
    52921 agccgggcga tatcggtgat gttcagcaaa ccgaacggga aaacctatca ggacatgctt
    52981 gatgttttta atgatcctga gcttatggaa gaattgaatc gaccgatctt ttattatccg
    53041 atcgtttatg atgtacatga actggaacaa aaatataaca tacgaacgga tgaagtgaca
    53101 tttattttct ttgatggagg aaaagaagca aatcggataa ctgcgggtaa gggaggaata
    53161 actgattttg ataaggagtt gatccctgaa ctgaatcgac ttccgttagc caatatcaaa
    53221 caattagaag aagaattgga tagaacggga gtaacttccc aaaattccga gattgctaat
    53281 tgagacattg gcgcgatgaa acggtgggtc tatatagcgc tatttttaat tatcagaaca
    53341 agtgatgttt gatcttcaaa ataacaaata aaaaattaaa aaatatagtt gacgaaattc
    53401 aaaaattcga ttagacttaa atcaattctt aaggaaaggg tgacctgtaa tgaacttatc
    53461 aaaacgtatt tcagcagaaa acaaaaaaaa ttggcgcagc tggcgtagat aagttgtatt
    53521 aatggtgaga ctttcccata gatacaactt tttagagtgc atttctaaag cttgtatcta
    53581 tgctagacat gaacattgac tgccgcatag attagaatct atgcggtttt ttgtgtaaag
    53641 acaatgaccg ctttggccat tgtctttttt tgtagactgg atacttgcct gattataata
    53701 aataaaaaga ttgactttgc aggaggaaaa aaatgaaaaa caaacgatta ataacagttg
    53761 tagtaattat tttagtttat ttagcaggtg catttattta tggaaaagcc tcaggcgaaa
    53821 aagaaagtac ttcgacgaac cagacaaaaa aaacaacagt tgttggggta ttgcaatacg
    53881 tgagccatcc ggcattagat gagatttatc gaggaatcaa agatggtctg aaagaagaag
    53941 gattaaaaga aggagaaaat cttgagatca aatttcaaaa tggacaagcc gatcagagca
    54001 agctagccac aatgagtcaa cagctagtac aatctgatcc agatgccttg attggaatcg
    54061 caacacctgc cgctcaatca ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg
    54121 ctgtaacgga tcctgtgggg gctggattag tcgcttcttt agagaaacct ggtggtaatg
    54181 taacaggtgt ttctgatcag ccgcctgtta cttcacaaat caagcttgct tctgatttgt
    54241 tgccaaatgc aaaaaaagtc ggaatcttgt atgcttcttc agaggataat tcaaaatacc
    54301 aagtagcaca agcagaagaa gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg
    54361 tgccttccac taatgagatt gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta
    54421 tctatgttcc attagataat accattgcca atgcgatgca aacagtggta aaagaagcaa
    54481 acaaagcaaa tatccccgtc attccatctg tggatacaat ggtggaacaa ggtggactag
    54541 caacaatcgg gatcaatcaa tatcaattag ggcttcaaag cggtaagatg gcggcagaac
    54601 ttgcgagtgg aaaagaaaaa ccggaaacga cgcctgttta tacatttgat caaggggata
    54661 cagtgatcaa tcaatctcaa gctgatcgct taggaatcat tgttccccaa tcgatgaagg
    54721 aaaaagccaa aatcattaca gatgactcgc agcaagaaac atctaaggag gatgacaaat
    54781 gatcgtatca gctatttcac aaggcatgct ttgggcaata ttaggattag ggattttcat
    54841 gacttatcgg atcttagact ttcctgatat gacgacagaa gggtccttcc cattaggtgc
    54901 tgctgtctgt gtgacagcta ttactcatgg ggtagcgccg attgtagcta cattattagg
    54961 cgtaggtgct ggcatgatag caggactcgt tacggggtta ctttatacaa aaggaaagat
    55021 accggttatt ttagcaggga ttttagtgat gtcaggattg aactcagtta ttttgtttgt
    55081 gatgcgaaca ccaaatcttt ctttattgaa tcatccgatt ctacaagaca gcttccatcg
    55141 ctttgggtta cctgattact ttgacacagt ccttcttggc ttcatctctt tgggcatctt
    55201 gattgctgta ctgctattct tcttcaatac agatttgggt caaggctata ttgcgacagg
    55261 agacaacgaa acgatggctc ggtcgttagg gataaaaacc gatcgtatga aaattttagg
    55321 tttaactttg tcgaacggtg tcattgcatt atccggtgcg ctgattgccc agaacgacgg
    55381 atatgcagat gtaaataaag ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat
    55441 cggtgaagtg atcttccaag aattgacgct agcagaaaga ttgattgcta ttgttgtcgg
    55501 cagtatcatt tatcagctat tgattttagt agtaatcaaa ctagggtttg acacgactta
    55561 tctgaagttg ttctccgcta tcatcttagc tatttgtctg atgatcccgc aattgaagaa
    55621 agcactgaac ttaaaaacgg gttttgagaa ggaggcatag taatgactga tatattgaca
    55681 atcaaaaatg gcagaaaagt cgtaaacaat ggaatgaatg aagagaaagt actattggat
    55741 gaaattaacc ttacaatcag agaaggggac ttcatcactg tccttggcgg gaatggtgct
    55801 ggaaaaagca cactgttcaa tacgatcgct ggtacgcttc aactcagtca aggatcgatc
    55861 tcttttaaag atcagctgat cacaaaagat tcagaagaaa aaagagcacg attcctttct
    55921 cgagttttcc aagaccccaa gatgggaacg gctcctcgga tgacagtagc agaaaatttg
    55981 ctacttgcac aaaaaagagg tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa
    56041 aaagaagatt ttttcagact atgtcaagaa gtcggtaatg gcctagagca acacttggat
    56101 actccagcgg gtgaattatc tggtggtcaa cgccaagcat taagcttgct gatggcgacg
    56161 atcacagctc ctgagttact attattagat gaacatactg cagctttaga tcctaaaaca
    56221 gctaaagcat taatgcgatt aacaaatcag cggatcaatg aacaaaaatt gacttgtctc
    56281 atgatcacgc atcgaatgga agacgccttg aaatacggaa atcgtctgat cgtactccaa
    56341 aaagggaaaa tcgttcaaga tatttctgaa aaagaaaagc aacatttgac aatgggcgac
    56401 cttttgcagt tctttgaaga agtagtggat tgatttcgac agaaaaattc ctttcagtag
    56461 aaggacttga aaactcattg acatcttcct ttgattcatc ctatacttaa aacaataaac
    56521 aattaaacat atttgggtgt tttgtaggat cacgtagtca atgattttaa caattaaata
    56581 gaatattctt tgctctgaca agtaaacgat gagaaaagaa tttgctactt tgccacctag
    56641 acgtttcgta ctttttcgta tcaaacgcta agtggctttt tttattgaag taagaacaaa
    56701 ggaggaataa gatgatttct ggacaaacca aattagctgg gctctttgct gatccggctt
    56761 ctcacagttt atccccattg atgcacaata ctgcttttga agcaaacaac atcgatgcgg
    56821 tgtatttagc tttccaagta aatccaatga atttgaagca agcagtcgaa agtattcgaa
    56881 cttttgatat gttaggtgta aatctttcga tgccgaataa aacagcggtt attccttatc
    56941 tggatgaggt aagccaggaa gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa
    57001 agggtcgttt gattggctat aatactgatg gtgcgggctt catgcaatcc gtgcaagaag
    57061 ctgggatttc gatcaaaaag aaaaaaatga ctctactggg agctggaggt gcagcaaagg
    57121 cagtcactgt tcaagcagcg ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca
    57181 accaaacatt cgctcaagta aaagaaacat tcgaacaact cagctatcga acttcttgta
    57241 agataaaagt tcgtgacttt gccgatacgc atcaattaag aaaagacttg aaagaaagct
    57301 gtcttctagt caatgcaaca gatatcggga tgggaaagaa aatcaatcag tcaccgctct
    57361 tagatataag catgatgcat gagaatctag cggtttttga tttgatttat gcacctagag
    57421 aaacacgttt attaaaagaa gcaaaaaaag taggagcaaa aggatacaat ggacttggca
    57481 tgttgatcta ccaaggagca atcgcgtttg aattatggac aaaggaaaag atgccgatcc
    57541 atgtgattga aaagctgttt gattaaaaaa ggataataga taaagatctt ttattctcgc
    57601 ataaacggtc tcaagaaaag taatccttta tgtatcgaag catttatttc gttatttttt
    57661 attaaattat caaaaaatga taggcttttg tcgattggtt atcgtatcct ttatttatca
    57721 gtaagaaacg aggaggaacc acaatgagtc ggatggatcg ctataaggac atccataaga
    57781 aggcgaaacc tctgaagcgg gatacgggga gtatttttgc tagaagagaa cgaaaacgaa
    57841 cgcgtatgga aaatgaggaa gatgctaccc gtatttatga aagagagcag cattcatttg
    57901 aacatttgaa ccaatacgag caacagatat acagagatgc accaagagca gaagaacaac
    57961 aaaaaaataa aaaatttttc aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa
    58021 agcgatcgtg gaaaaaaatc attctatcga ttttgcttgt tttgatcctt tattcaggta
    58081 tctcattttt tattggtaaa tatgtagctg aacatgacac aagcttagag gcagcgactt
    58141 cagaaacctt tcatgggcaa aaagcgacga atggtgcgca taatattttg atccttggaa
    58201 gtgatacacg cggagaagat gccgggcgag ccgatacgat catggtctta cagttggatg
    58261 gtccttccca taagccgaaa ctgatttctt ttatgcgtga cagcttcgtt acgatcccag
    58321 gggtaggaca aaataagatc aattcagctt atgcatacgg aggagccgat ctagtgcgtc
    58381 aaactttagt agaaaatttt ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat
    58441 cgtttgaaaa agtgatcgat gccttattca tgaacggagt gaagatcgat gcagaaaaag
    58501 atctgaatct ggatggtgta gatattaaaa aagggggcca gaagatggat ggacatgttc
    58561 ttttgcagta cgcacgtttc cgaatggatg aacaaggaga ctttggccgt gtcagacgcc
    58621 aacagcaagt aatgaacgct attttcagcc aattgaagaa tccattgaat ctgatccgtg
    58681 caccttatgc agctggtaaa gctttaggat atacatccac agatgtcact gcttggttct
    58741 tgacgaaaaa cctcttttcg attgcacgag gagctggtgg cattgatcga ataagtgtgc
    58801 cagtagacgg ttcgtggaat tatgggcaaa gtgattatgc tggcagtatt cttgaaattg
    58861 ataatcaaat gaatcaatct gctattacag attttttgaa taaatgatca taaaaaagat
    58921 caagaagaag ctgcgcaagg cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa
    58981 cgctttctta tggtagattt aaccaagaga ggagctgttt acattggata tctatctgaa
    59041 cttcaaaaat gagtcgcgtg aagccatcgc tttttatgaa aaagtttttc agactgaagc
    59101 atcaggtatc accacatacg gtgaaatgcc tggaatagaa caaatgaact tgccagaaga
    59161 agcaaaaaat ctaatcacga atgcacggct gaacatttat gatacattag ttatgtttgc
    59221 agatacgcca gaaggaattg gtcctgaatt agttgtggga aataatgttt ctttagtcat
    59281 ccagacaaga aatgaagaag aaacgaagcg cctatttgaa agcttagctg agggtggtaa
    59341 gattgagatg ccactggatg ctgttccgtg ggctcagttg tatgggatgg taactgatcg
    59401 atttggcatc aactggcaga ttaaccaaga gtgacagcta aagatgaata ttttctggaa
    59461 acatcaagca gcaaacggat catcgtttgt ttgcttgatg tttttgtgtc tatttcgact
    59521 atacggtatc ttttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc
    59581 aaaaaattaa gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca
    59641 caagcaaaag cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa
    59701 atcagcgatc cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta
    59761 gatatggttg ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg
    59821 cctattttcc cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg
    59881 atcgtaaaac gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga
    59941 gaaggtgaag ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag
    60001 aactatttac gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag
    60061 aatctccaac ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac
    60121 gctgattggc tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa
    60181 gggattgatg gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat
    60241 cagctgcaag aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta
    60301 attacgacaa acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc
    60361 caagaagagt taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga
    60421 cggattcttg aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta
    60481 tctagtttgc agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat
    60541 gctgctcaag gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac
    60601 attacagaaa atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta
    60661 ggaatagaag aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa
    60721 aaagttcaag aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt
    60781 gcgaagcttt ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct
    60841 atgtttgctg cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta
    60901 aagctaaaaa gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc
    60961 aagtcaaaaa agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa
    61021 gtagaactga aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg
    61081 ttgatcacag caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt
    61141 ctcaaagaag tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt
    61201 aagcagaagc attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc
    61261 actctttgta aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc
    61321 tgggaagggt acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag
    61381 aatatcaaaa aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta
    61441 gacttttcag atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat
    61501 caattaggaa gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct
    61561 acatgcttga atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg
    61621 actaaaacga atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt
    61681 tttactggta aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc
    61741 ggtttttctt ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa
    61801 acatcaagaa aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat
    61861 atttattccg aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa
    61921 gcaaaaacgt tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa
    61981 aagtgcattc atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag
    62041 catttctaaa gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag
    62101 catattcacc aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt
    62161 tttttcataa aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg
    62221 tggtagcggc tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat
    62281 attttggtta agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt
    62341 cattttttcg taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg
    62401 gatataatgt acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa
    62461 tgaaaaagta taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag
    62521 taaaagaatt catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg
    62581 gttctgtacg aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga
    62641 tcatgaatca atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac
    62701 taaagaaatt atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat
    62761 cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt
    62821 caatcaagaa ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc
    62881 taaaaaacat ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt
    62941 tcttcaattg aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat
    63001 aatgattacc actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt
    63061 acgacaaacc gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg
    63121 tcattaagga atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa
    63181 atatggaaaa tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa
    63241 tagtaggaaa tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata
    63301 atatatatta tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc
    63361 aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt
    63421 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
    63481 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
    63541 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
    63601 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
    63661 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
    63721 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
    63781 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
    63841 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
    63901 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
    63961 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
    64021 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
    64081 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
    64141 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
    64201 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
    64261 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
    64321 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
    64381 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
    64441 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
    64501 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
    64561 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
    64621 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
    64681 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
    64741 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
    64801 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
    64861 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
    64921 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
    64981 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
    65041 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
    65101 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
    65161 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
    65221 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
    65281 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
    65341 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
    65401 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
    65461 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
    65521 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
    65581 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
    65641 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
    65701 ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
    65761 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
    65821 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
    65881 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
    65941 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
    66001 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
    66061 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
    66121 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
    66181 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
    66241 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
    66301 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
    66361 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
    66421 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
    66481 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
    66541 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
    66601 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
    66661 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
    66721 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
    66781 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
    66841 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
    66901 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
    66961 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
    67021 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
    67081 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
    67141 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
    67201 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
    67261 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
    67321 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
    67381 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
    67441 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
    67501 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
    67561 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
    67621 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
    67681 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
    67741 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
    67801 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
    67861 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
    67921 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
    67981 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
    68041 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
    68101 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
    68161 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
    68221 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
    68281 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
    68341 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
    68401 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
    68461 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
    68521 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
    68581 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
    68641 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
    68701 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
    68761 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
    68821 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
    68881 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
    68941 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
    69001 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
    69061 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
    69121 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
    69181 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
    69241 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
    69301 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
    69361 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
    69421 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
    69481 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
    69541 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
    69601 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
    69661 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
    69721 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
    69781 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
    69841 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
    69901 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
    69961 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
    70021 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
    70081 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
    70141 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
    70201 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
    70261 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
    70321 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
    70381 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
    70441 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
    70501 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
    70561 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
    70621 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
    70681 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
    70741 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
    70801 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
    70861 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
    70921 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
    70981 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
    71041 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
    71101 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
    71161 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
    71221 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
    71281 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
    71341 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
    71401 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
    71461 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
    71521 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
    71581 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
    71641 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
    71701 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
    71761 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
    71821 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
    71881 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
    71941 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
    72001 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
    72061 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
    72121 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
    72181 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
    72241 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
    72301 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
    72361 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
    72421 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
    72481 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
    72541 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
    72601 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
    72661 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
    72721 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
    72781 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
    72841 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
    72901 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
    72961 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
    73021 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
    73081 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
    73141 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
    73201 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
    73261 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
    73321 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
    73381 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
    73441 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
    73501 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    73561 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    73621 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    73681 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    73741 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    73801 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    73861 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    73921 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    73981 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    74041 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    74101 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    74161 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    74221 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    74281 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    74341 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    74401 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    74461 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    74521 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    74581 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    74641 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    74701 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    74761 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    74821 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    74881 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    74941 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    75001 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    75061 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    75121 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    75181 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    75241 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    75301 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    75361 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    75421 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    75481 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    75541 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    75601 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    75661 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    75721 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    75781 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    75841 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    75901 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    75961 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    76021 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    76081 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    76141 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    76201 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    76261 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    76321 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    76381 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    76441 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    76501 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    76561 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    76621 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    76681 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    76741 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    76801 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    76861 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    76921 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    76981 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    77041 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    77101 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    77161 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    77221 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    77281 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    77341 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    77401 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    77461 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    77521 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    77581 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    77641 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    77701 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    77761 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    77821 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    77881 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    77941 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    78001 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    78061 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    78121 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    78181 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    78241 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    78301 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    78361 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    78421 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    78481 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    78541 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    78601 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    78661 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    78721 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    78781 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    78841 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    78901 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    78961 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    79021 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    79081 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    79141 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    79201 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    79261 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    79321 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    79381 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    79441 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    79501 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    79561 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    79621 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    79681 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    79741 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    79801 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    79861 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    79921 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    79981 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    80041 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    80101 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    80161 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    80221 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    80281 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    80341 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    80401 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    80461 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    80521 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    80581 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    80641 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    80701 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    80761 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    80821 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    80881 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    80941 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    81001 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    81061 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    81121 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    81181 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    81241 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    81301 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    81361 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    81421 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    81481 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    81541 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    81601 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    81661 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    81721 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    81781 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    81841 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    81901 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    81961 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    82021 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    82081 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    82141 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    82201 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    82261 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    82321 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    82381 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    82441 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    82501 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    82561 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    82621 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    82681 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    82741 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    82801 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    82861 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    82921 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    82981 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    83041 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    83101 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    83161 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    83221 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    83281 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    83341 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    83401 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    83461 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    83521 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    83581 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    83641 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    83701 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    83761 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    83821 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    83881 tgttggaacg aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca
    83941 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    84001 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    84061 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    84121 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    84181 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    84241 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    84301 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    84361 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    84421 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    84481 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    84541 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    84601 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    84661 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    84721 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aaacgaagaa
    84781 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
    84841 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
    84901 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
    84961 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
    85021 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
    85081 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
    85141 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
    85201 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
    85261 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
    85321 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
    85381 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
    85441 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
    85501 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
    85561 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
    85621 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
    85681 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
    85741 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
    85801 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
    85861 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
    85921 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
    85981 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
    86041 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
    86101 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
    86161 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
    86221 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
    86281 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
    86341 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
    86401 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
    86461 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
    86521 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
    86581 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
    86641 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
    86701 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
    86761 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
    86821 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
    86881 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
    86941 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
    87001 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
    87061 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
    87121 tgtcccaata gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga
    87181 atcctttatt acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc
    87241 atacttcaaa gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata
    87301 agtttcagga aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt
    87361 atctccaatt ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt
    87421 tttaaattca tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa
    87481 aactgtaaca ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc
    87541 tttctctcct ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac
    87601 ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt
    87661 aaactcttct gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa
    87721 gaacataata ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc
    87781 tagaaacatt tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga
    87841 atagtgtaac tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt
    87901 gtctaagaca tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta
    87961 tttatgcttt ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac
    88021 tattagtagt agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa
    88081 tcggcgagat aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag
    88141 tttatgatct tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag
    88201 caaaaatacg gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt
    88261 tggaagataa gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct
    88321 atcgagaaga actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg
    88381 atgaaaatgt tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt
    88441 ataaagaaca agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac
    88501 aaaataaggg aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt
    88561 ctgatacgac gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa
    88621 gtactggatt ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg
    88681 tgtgatgcaa tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat
    88741 gtagcagcat ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac
    88801 caatctgcat acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca
    88861 gtacaattag agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg
    88921 gaacaaaaca gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac
    88981 actcaagtga tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg
    89041 cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat
    89101 attgaattaa atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa
    89161 gcggagacga atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc
    89221 aagaattcat tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt
    89281 ctctctattg gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct
    89341 tggagaagac aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg
    89401 attattcaaa atagtttgga acatcttttt accaagccta atcatactat tattgaagaa
    89461 tccgaaagca tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat
    89521 ttgctgacaa tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat
    89581 acacaagaat atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga
    89641 aaagagttta ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc
    89701 catcaagttt tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag
    89761 atcattttgg attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc
    89821 ccttctatca gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa
    89881 tctcgggcga aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc
    89941 gaacagcatc atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa
    90001 gtccaattgc cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa
    90061 aatcttgtca cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg
    90121 aaataagccg aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt
    90181 acgatttttt attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt
    90241 ctcggctaac atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa
    90301 cttcttcacg aataactgta tgtttgctta catctacttg ctcttctttg attgggatag
    90361 taatcgtgtc atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt
    90421 tgcgttcgat gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga
    90481 ccacatgttt gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt
    90541 tttcttcttt tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag
    90601 tcttgcttgt acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg
    90661 tcatattcaa tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat
    90721 ctgttccagt gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt
    90781 gtgtatccat tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa
    90841 attttccatc agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg
    90901 tctcagaatc ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt
    90961 cttcttcacc atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa
    91021 atcctagtct agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc
    91081 cttcgttacg caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga
    91141 tgaccgttaa atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt
    91201 atattgttac ttataaattt attctatagg agacg
//
LOCUS       DACEJZ010000002        81301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEJZ010000002 DACEJZ010000000
VERSION     DACEJZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.002"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..928)
                     /locus_tag="IW656_000084"
     CDS             complement(134..928)
                     /locus_tag="IW656_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAQ0835819.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1547..4381
                     /locus_tag="IW656_000085"
     CDS             1547..4381
                     /locus_tag="IW656_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0835820.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4572..5063
                     /locus_tag="IW656_000086"
     CDS             4572..5063
                     /locus_tag="IW656_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0835821.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5166..6155
                     /gene="manX"
                     /locus_tag="IW656_000087"
     CDS             5166..6155
                     /gene="manX"
                     /locus_tag="IW656_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0835822.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6188..6991
                     /locus_tag="IW656_000088"
     CDS             6188..6991
                     /locus_tag="IW656_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0835823.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7012..7926
                     /locus_tag="IW656_000089"
     CDS             7012..7926
                     /locus_tag="IW656_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0835824.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8009..8383
                     /locus_tag="IW656_000090"
     CDS             8009..8383
                     /locus_tag="IW656_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0835825.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8522..8767
                     /locus_tag="IW656_000091"
     CDS             8522..8767
                     /locus_tag="IW656_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0835826.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8939..10003
                     /locus_tag="IW656_000092"
     CDS             8939..10003
                     /locus_tag="IW656_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0835827.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            10249..10674
                     /locus_tag="IW656_000093"
     CDS             10249..10674
                     /locus_tag="IW656_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0835828.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10667..11116
                     /gene="nusB"
                     /locus_tag="IW656_000094"
     CDS             10667..11116
                     /gene="nusB"
                     /locus_tag="IW656_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0835829.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            11213..12067
                     /locus_tag="IW656_000095"
     CDS             11213..12067
                     /locus_tag="IW656_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0835830.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            12068..13405
                     /locus_tag="IW656_000096"
     CDS             12068..13405
                     /locus_tag="IW656_000096"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0835831.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            13410..13634
                     /locus_tag="IW656_000097"
     CDS             13410..13634
                     /locus_tag="IW656_000097"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0835832.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13636..14526
                     /locus_tag="IW656_000098"
     CDS             13636..14526
                     /locus_tag="IW656_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0835833.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14535..15353
                     /locus_tag="IW656_000099"
     CDS             14535..15353
                     /locus_tag="IW656_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0835834.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15460..15909
                     /locus_tag="IW656_000100"
     CDS             15460..15909
                     /locus_tag="IW656_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0835835.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15927..17609
                     /gene="recN"
                     /locus_tag="IW656_000101"
     CDS             15927..17609
                     /gene="recN"
                     /locus_tag="IW656_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0835836.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17664..17780)
                     /locus_tag="IW656_000102"
     CDS             complement(17664..17780)
                     /locus_tag="IW656_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0835837.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17853..18809)
                     /locus_tag="IW656_000103"
     CDS             complement(17853..18809)
                     /locus_tag="IW656_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0835838.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18980..19348
                     /locus_tag="IW656_000104"
     CDS             18980..19348
                     /locus_tag="IW656_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0835839.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19518..19949
                     /gene="mraZ"
                     /locus_tag="IW656_000105"
     CDS             19518..19949
                     /gene="mraZ"
                     /locus_tag="IW656_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0835840.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19970..20929
                     /gene="rsmH"
                     /locus_tag="IW656_000106"
     CDS             19970..20929
                     /gene="rsmH"
                     /locus_tag="IW656_000106"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0835841.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20934..21335
                     /gene="ftsL"
                     /locus_tag="IW656_000107"
     CDS             20934..21335
                     /gene="ftsL"
                     /locus_tag="IW656_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0835842.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            21336..23528
                     /locus_tag="IW656_000108"
     CDS             21336..23528
                     /locus_tag="IW656_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0835843.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23554..24516
                     /locus_tag="IW656_000109"
     CDS             23554..24516
                     /locus_tag="IW656_000109"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0835844.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24530..25891
                     /locus_tag="IW656_000110"
     CDS             24530..25891
                     /locus_tag="IW656_000110"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0835845.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25917..27005
                     /gene="murG"
                     /locus_tag="IW656_000111"
     CDS             25917..27005
                     /gene="murG"
                     /locus_tag="IW656_000111"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0835846.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            27020..28240
                     /locus_tag="IW656_000112"
     CDS             27020..28240
                     /locus_tag="IW656_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0835847.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            28396..29721
                     /gene="ftsA"
                     /locus_tag="IW656_000113"
     CDS             28396..29721
                     /gene="ftsA"
                     /locus_tag="IW656_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0835848.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29744..30985
                     /gene="ftsZ"
                     /locus_tag="IW656_000114"
     CDS             29744..30985
                     /gene="ftsZ"
                     /locus_tag="IW656_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0835849.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            31002..31679
                     /locus_tag="IW656_000115"
     CDS             31002..31679
                     /locus_tag="IW656_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0835850.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31693..32292
                     /locus_tag="IW656_000116"
     CDS             31693..32292
                     /locus_tag="IW656_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0835851.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            32307..32594
                     /locus_tag="IW656_000117"
     CDS             32307..32594
                     /locus_tag="IW656_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0835852.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32643..33425
                     /locus_tag="IW656_000118"
     CDS             32643..33425
                     /locus_tag="IW656_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0835853.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            33446..34153
                     /locus_tag="IW656_000119"
     CDS             33446..34153
                     /locus_tag="IW656_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0835854.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            34415..37213
                     /gene="ileS"
                     /locus_tag="IW656_000120"
     CDS             34415..37213
                     /gene="ileS"
                     /locus_tag="IW656_000120"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0835855.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(37262..38788)
                     /locus_tag="IW656_000121"
     CDS             complement(37262..38788)
                     /locus_tag="IW656_000121"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0835856.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38803..39450)
                     /locus_tag="IW656_000122"
     CDS             complement(38803..39450)
                     /locus_tag="IW656_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0835857.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39634..39963)
                     /locus_tag="IW656_000123"
     CDS             complement(39634..39963)
                     /locus_tag="IW656_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0835858.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            40140..40868
                     /gene="sfsA"
                     /locus_tag="IW656_000124"
     CDS             40140..40868
                     /gene="sfsA"
                     /locus_tag="IW656_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0835859.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40884..41897
                     /locus_tag="IW656_000125"
     CDS             40884..41897
                     /locus_tag="IW656_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0835860.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41897..43174
                     /locus_tag="IW656_000126"
     CDS             41897..43174
                     /locus_tag="IW656_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0835861.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            43237..45939
                     /locus_tag="IW656_000127"
     CDS             43237..45939
                     /locus_tag="IW656_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0835862.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            46091..46408
                     /locus_tag="IW656_000128"
     CDS             46091..46408
                     /locus_tag="IW656_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0835863.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            46438..46758
                     /locus_tag="IW656_000129"
     CDS             46438..46758
                     /locus_tag="IW656_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0835864.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46866..48326
                     /locus_tag="IW656_000130"
     CDS             46866..48326
                     /locus_tag="IW656_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0835865.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            48394..48615
                     /locus_tag="IW656_000131"
     CDS             48394..48615
                     /locus_tag="IW656_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835866.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48646..48828
                     /locus_tag="IW656_000132"
     CDS             48646..48828
                     /locus_tag="IW656_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0835867.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48828..49241
                     /locus_tag="IW656_000133"
     CDS             48828..49241
                     /locus_tag="IW656_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0835868.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            49364..50545
                     /locus_tag="IW656_000134"
     CDS             49364..50545
                     /locus_tag="IW656_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0835869.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            50934..51007
                     /locus_tag="IW656_000135"
     tRNA            50934..51007
                     /locus_tag="IW656_000135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50968..50970,aa:Arg,seq:cct)
     gene            complement(51076..52215)
                     /locus_tag="IW656_000136"
     CDS             complement(51076..52215)
                     /locus_tag="IW656_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0835870.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(52514..53149)
                     /locus_tag="IW656_000137"
     CDS             complement(52514..53149)
                     /locus_tag="IW656_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835871.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(53262..53897)
                     /locus_tag="IW656_000138"
     CDS             complement(53262..53897)
                     /locus_tag="IW656_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ0835872.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(53931..54392)
                     /locus_tag="IW656_000139"
     CDS             complement(53931..54392)
                     /locus_tag="IW656_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ0835873.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(54522..54953)
                     /locus_tag="IW656_000140"
     CDS             complement(54522..54953)
                     /locus_tag="IW656_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0835874.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(54971..55291)
                     /locus_tag="IW656_000141"
     CDS             complement(54971..55291)
                     /locus_tag="IW656_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0835875.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            55590..56366
                     /locus_tag="IW656_000142"
     CDS             55590..56366
                     /locus_tag="IW656_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0835876.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            56381..56584
                     /locus_tag="IW656_000143"
     CDS             56381..56584
                     /locus_tag="IW656_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0835877.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56600..56938
                     /locus_tag="IW656_000144"
     CDS             56600..56938
                     /locus_tag="IW656_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ0835878.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            56925..57104
                     /locus_tag="IW656_000145"
     CDS             56925..57104
                     /locus_tag="IW656_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835879.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(57147..57617)
                     /locus_tag="IW656_000146"
     CDS             complement(57147..57617)
                     /locus_tag="IW656_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835880.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            57704..58402
                     /locus_tag="IW656_000147"
     CDS             57704..58402
                     /locus_tag="IW656_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0835881.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            58580..58921
                     /locus_tag="IW656_000148"
     CDS             58580..58921
                     /locus_tag="IW656_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835882.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            58914..59585
                     /locus_tag="IW656_000149"
     CDS             58914..59585
                     /locus_tag="IW656_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0835883.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            59591..60277
                     /locus_tag="IW656_000150"
     CDS             59591..60277
                     /locus_tag="IW656_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835884.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            60280..61029
                     /locus_tag="IW656_000151"
     CDS             60280..61029
                     /locus_tag="IW656_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0835885.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            61041..61310
                     /locus_tag="IW656_000152"
     CDS             61041..61310
                     /locus_tag="IW656_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835886.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            61473..61772
                     /locus_tag="IW656_000153"
     CDS             61473..61772
                     /locus_tag="IW656_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835887.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            61772..62074
                     /locus_tag="IW656_000154"
     CDS             61772..62074
                     /locus_tag="IW656_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835888.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            62114..62479
                     /locus_tag="IW656_000155"
     CDS             62114..62479
                     /locus_tag="IW656_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835889.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            63106..63294
                     /locus_tag="IW656_000156"
     CDS             63106..63294
                     /locus_tag="IW656_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835890.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            63302..63496
                     /locus_tag="IW656_000157"
     CDS             63302..63496
                     /locus_tag="IW656_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835891.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(63529..63741)
                     /locus_tag="IW656_000158"
     CDS             complement(63529..63741)
                     /locus_tag="IW656_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835892.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            63866..64069
                     /locus_tag="IW656_000159"
     CDS             63866..64069
                     /locus_tag="IW656_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835893.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            64066..64338
                     /locus_tag="IW656_000160"
     CDS             64066..64338
                     /locus_tag="IW656_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAQ0835894.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            64339..64524
                     /locus_tag="IW656_000161"
     CDS             64339..64524
                     /locus_tag="IW656_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835895.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            64511..64858
                     /locus_tag="IW656_000162"
     CDS             64511..64858
                     /locus_tag="IW656_000162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835896.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            64818..65294
                     /locus_tag="IW656_000163"
     CDS             64818..65294
                     /locus_tag="IW656_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAQ0835897.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(65440..66168)
                     /locus_tag="IW656_000164"
     CDS             complement(65440..66168)
                     /locus_tag="IW656_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAQ0835898.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            66216..66494
                     /locus_tag="IW656_000165"
     CDS             66216..66494
                     /locus_tag="IW656_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835899.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            66496..66882
                     /locus_tag="IW656_000166"
     CDS             66496..66882
                     /locus_tag="IW656_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835900.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            66879..67259
                     /locus_tag="IW656_000167"
     CDS             66879..67259
                     /locus_tag="IW656_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0835901.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            67371..67823
                     /locus_tag="IW656_000168"
     CDS             67371..67823
                     /locus_tag="IW656_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAQ0835902.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            67820..69547
                     /locus_tag="IW656_000169"
     CDS             67820..69547
                     /locus_tag="IW656_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0835903.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            69611..70798
                     /locus_tag="IW656_000170"
     CDS             69611..70798
                     /locus_tag="IW656_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0835904.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            70764..71339
                     /locus_tag="IW656_000171"
     CDS             70764..71339
                     /locus_tag="IW656_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAQ0835905.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            71352..72716
                     /locus_tag="IW656_000172"
     CDS             71352..72716
                     /locus_tag="IW656_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0835906.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            72718..72999
                     /locus_tag="IW656_000173"
     CDS             72718..72999
                     /locus_tag="IW656_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0835907.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            72977..73303
                     /locus_tag="IW656_000174"
     CDS             72977..73303
                     /locus_tag="IW656_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0835908.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            73293..73631
                     /locus_tag="IW656_000175"
     CDS             73293..73631
                     /locus_tag="IW656_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835909.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            73621..73986
                     /locus_tag="IW656_000176"
     CDS             73621..73986
                     /locus_tag="IW656_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0835910.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            73993..74619
                     /locus_tag="IW656_000177"
     CDS             73993..74619
                     /locus_tag="IW656_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0835911.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            74619..75083
                     /locus_tag="IW656_000178"
     CDS             74619..75083
                     /locus_tag="IW656_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835912.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            75278..77587
                     /locus_tag="IW656_000179"
     CDS             75278..77587
                     /locus_tag="IW656_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0835913.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            77599..78303
                     /locus_tag="IW656_000180"
     CDS             77599..78303
                     /locus_tag="IW656_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0835914.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            78300..81059
                     /locus_tag="IW656_000181"
     CDS             78300..81059
                     /locus_tag="IW656_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0835915.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            81072..>81301
                     /locus_tag="IW656_000182"
     CDS             81072..>81301
                     /locus_tag="IW656_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0835916.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKI"
BASE COUNT        27518 a        13345 c        16998 g        23440 t
ORIGIN      
        1 aatttaaacc ataattgtgt ttttccttcc attaacatat aaagttcacc tcaaaaagag
       61 tatacgaaca aatgtttgta ttgtaaatag aaaaagatga gctaaattaa atgcccatct
      121 tctttgatag atattataaa tctaataatt cttgtttctt tttattaaat tcttcttctg
      181 taatgattcc atcatccaat agttctttat attttcttat ttctgaagct gaggaaattg
      241 ttgatgtatt ggaatttgta gcagaaatat ttcttctttt taaattataa ataatttcgt
      301 ttaaagaatc acttatccta tcaacagtat ctttatcaac tgcaacattc aatactttac
      361 caagagtatc aatagttaat gtacccaaca gcactcctct ttttttacta atattattta
      421 aattgtctaa tataatactt tgtacattat tgcctatcat tttttgttga gctacaatta
      481 aacgtttatt agttaatgcg tatgcataat tattgtcatg cttagttgat gagatataat
      541 tatgcaatcc tacaaagcag aagattacgt attcgtcact gttaagttga tctgatatta
      601 agttaaaatg atgagttgtc cattttttag tcattccttt accgtagccg tatttaagac
      661 aaaattctac catctcttca gtggttctca ttctatcatt acctatatca tgattgtttt
      721 gtgcagtatg atcagatatg tttaattttc ttttttctga tggatcctta tctaaatctt
      781 ctaaattcaa agatcttatt attttataag gagtagtcat accgtaacca gcttttttca
      841 tacaattgcc gcaaacaatt tgtttatctt tgattttata tgaagttaag ccaattttac
      901 ctccgcactt tgcacattgt ttactcacat tagttcctcc tagatatcag ctttctacta
      961 ctattaacat tctatcaata gtgaccatac tttgaccata ctttttagaa agaatagaaa
     1021 attccaagat atttaataat agaagaaaag cactaacatc atgggttcag aagaattgag
     1081 agcttatcag gaaacagtat aattgaagtg tctgtttatc cagtagctca agaagcttaa
     1141 ttgaatctta tgattaaaga ggttgtgaca agacttttgt cacagccttt tttgtttata
     1201 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
     1261 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
     1321 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
     1381 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
     1441 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
     1501 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
     1561 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
     1621 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
     1681 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
     1741 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
     1801 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
     1861 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
     1921 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
     1981 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
     2041 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
     2101 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
     2161 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     2221 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     2281 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     2341 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     2401 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     2461 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     2521 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     2581 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     2641 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     2701 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     2761 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     2821 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     2881 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     2941 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     3001 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     3061 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     3121 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     3181 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     3241 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     3301 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     3361 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     3421 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     3481 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     3541 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     3601 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     3661 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     3721 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     3781 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     3841 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     3901 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     3961 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     4021 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     4081 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     4141 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     4201 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     4261 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     4321 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     4381 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     4441 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     4501 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     4561 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     4621 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     4681 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     4741 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     4801 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     4861 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     4921 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     4981 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     5041 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     5101 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     5161 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     5221 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     5281 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     5341 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     5401 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     5461 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     5521 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     5581 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     5641 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     5701 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     5761 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     5821 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     5881 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     5941 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     6001 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     6061 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     6121 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     6181 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     6241 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     6301 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     6361 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     6421 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     6481 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     6541 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     6601 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     6661 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     6721 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     6781 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     6841 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     6901 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     6961 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     7021 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     7081 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     7141 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     7201 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     7261 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     7321 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     7381 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     7441 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     7501 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     7561 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     7621 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     7681 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     7741 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     7801 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     7861 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     7921 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     7981 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     8041 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     8101 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     8161 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     8221 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     8281 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     8341 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
     8401 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
     8461 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
     8521 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
     8581 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
     8641 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
     8701 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
     8761 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
     8821 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
     8881 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
     8941 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
     9001 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
     9061 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
     9121 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
     9181 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
     9241 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
     9301 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
     9361 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
     9421 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
     9481 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
     9541 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
     9601 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
     9661 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
     9721 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
     9781 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
     9841 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
     9901 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
     9961 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
    10021 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
    10081 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
    10141 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
    10201 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
    10261 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
    10321 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
    10381 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
    10441 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
    10501 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
    10561 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
    10621 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
    10681 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
    10741 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
    10801 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
    10861 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
    10921 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
    10981 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
    11041 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt
    11101 agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc
    11161 gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga
    11221 attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca
    11281 aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc
    11341 agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc
    11401 attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa
    11461 atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat
    11521 agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc
    11581 gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg
    11641 catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat
    11701 cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac
    11761 tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat
    11821 tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc
    11881 tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa
    11941 atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc
    12001 aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg
    12061 tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa
    12121 atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg
    12181 tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc
    12241 tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt
    12301 ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga
    12361 gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa
    12421 gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg
    12481 aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa
    12541 aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc
    12601 agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat
    12661 gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt
    12721 tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga
    12781 taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga
    12841 attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt
    12901 gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg
    12961 ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca
    13021 gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac
    13081 gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg
    13141 ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca
    13201 acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat
    13261 catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga
    13321 gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt
    13381 acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc
    13441 aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc
    13501 tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag
    13561 cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa
    13621 acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa
    13681 aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg
    13741 agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt
    13801 gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg
    13861 gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt
    13921 agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat
    13981 ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag
    14041 cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa
    14101 gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat
    14161 gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag
    14221 caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt
    14281 cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct
    14341 ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca
    14401 cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag
    14461 gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag
    14521 aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat
    14581 ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa
    14641 acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag
    14701 gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat
    14761 ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta
    14821 ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc
    14881 agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc
    14941 gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt
    15001 cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag
    15061 aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg
    15121 gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg
    15181 cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa
    15241 atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta
    15301 ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa
    15361 ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta
    15421 tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat
    15481 cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa
    15541 atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa
    15601 ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa
    15661 acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt
    15721 gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca
    15781 aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat
    15841 gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat
    15901 tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga
    15961 ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg
    16021 gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa
    16081 gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa
    16141 aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc
    16201 tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata
    16261 ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa
    16321 acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag
    16381 atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc
    16441 tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata
    16501 tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga
    16561 aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg
    16621 caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg
    16681 tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata
    16741 tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt
    16801 tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac
    16861 agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca
    16921 ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac
    16981 tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa
    17041 aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa
    17101 acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat
    17161 ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag cctttggtga
    17221 aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt
    17281 ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag
    17341 ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca
    17401 tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag
    17461 tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag
    17521 aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag
    17581 aactattgaa actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag
    17641 cagctgatag ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat
    17701 ccaatcgtta aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt
    17761 gtttgttttt tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac
    17821 aatctttttt ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa
    17881 taggtagtta cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc
    17941 ctttcagcaa taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata
    18001 gttaaaataa tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg
    18061 attgctgaaa aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc
    18121 attgcctgct ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct
    18181 tggaaaagtt tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct
    18241 ctgaaataaa cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg
    18301 gatacttgaa gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg
    18361 ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata
    18421 tcactttttg gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta
    18481 attgtgataa gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga
    18541 aaataaccgc taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt
    18601 gtcaactttg attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa
    18661 gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta
    18721 ggacgttcta cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc
    18781 tcattatcta atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt
    18841 ataacggata gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca
    18901 taaatttaaa taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat
    18961 aaaaaaggag tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta
    19021 cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg
    19081 ttttaaaata aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt
    19141 gtctagaaat acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat
    19201 gggaatttgt attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta
    19261 tttcaagatg acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat
    19321 cattgtgaac atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa
    19381 aaggcttttt gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg
    19441 atttcccaga aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga
    19501 ggaatggggg gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac
    19561 gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg
    19621 gattggatgg ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac
    19681 tgaacgaaat gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg
    19741 ctgccactga atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa
    19801 attatgcagc tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt
    19861 gggatgagac acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg
    19921 ctgaaagtat gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac
    19981 atttcaacac tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga
    20041 tggcgtttat gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca
    20101 actaagttct aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc
    20161 caagctgcgt ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt
    20221 cagagaatta gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga
    20281 tctaggggtt tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga
    20341 tgcaccgttg gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa
    20401 tgaatattcc taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc
    20461 caaacaaatt gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg
    20521 tgagcttgtc gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca
    20581 tcctgcaaaa agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga
    20641 agaggcctct ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat
    20701 cacattccat tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt
    20761 acaagatcta ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt
    20821 cattactcgt aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg
    20881 cagtgcaaag ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag
    20941 aattaaagaa aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac
    21001 aacaaatgcc tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac
    21061 gattaaagca gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg
    21121 ttgctttatg tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa
    21181 tcacgtcaat acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag
    21241 aaaaaaacga actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt
    21301 ctattaatga cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc
    21361 ggtttatgga aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt
    21421 tatttgctac gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta
    21481 ttggaggtca tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa
    21541 gtgaagtagt aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag
    21601 aagatgcctc ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata
    21661 aaaaactgta tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc
    21721 tttctataga caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt
    21781 atcaagttga atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg
    21841 aagcggacat gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga
    21901 tgtatccaaa tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg
    21961 agaatggctt agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta
    22021 aagatggtaa gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg
    22081 cagaagaaga aaaagctgtc gacggacaag atatctacac gactttagac agcagactac
    22141 aaagttattt agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga
    22201 cagcagtcct gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt
    22261 tcaatcctga aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg
    22321 tacaagatag ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg
    22381 aagaaggaga attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg
    22441 ctacgatcaa tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt
    22501 catggtcaag taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata
    22561 actatttaca aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa
    22621 acggtgcttt acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg
    22681 taggtgtgac gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa
    22741 tgattcaacc tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa
    22801 ctcaaacaga agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat
    22861 atatgagaga tgttgtcgaa agcgaaaact acggtagtgc ttatggtgta tatagtgtac
    22921 ctggatacaa tgtatcggca aaaaacggaa cagcccaaat cgcttctgat acagggggat
    22981 atcaaacagg agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc
    23041 cagactatgt cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg
    23101 ctaaaatcgc aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt
    23161 cagatacaga aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg
    23221 acgttgcagc agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta
    23281 aaaaagtaca aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga
    23341 tcctatatac tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata
    23401 tcatgaagct ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg
    23461 tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag
    23521 aagaataaga agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc
    23581 attgtcagca gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa
    23641 atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc
    23701 ggaactccta caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg
    23761 gttggagctt ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg
    23821 ttatacggag taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg
    23881 ggactgaact cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt
    23941 gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta
    24001 ggaatcattt atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat
    24061 ctgacagatg gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac
    24121 ggtctaattg cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta
    24181 ggaggtttac tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat
    24241 gtcggttcat tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa
    24301 tggacattgc ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa
    24361 gttacttcct ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac
    24421 tttgaaatgt gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt
    24481 gtgacttcgt tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat
    24541 atcaacatat gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc
    24601 ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga
    24661 cgaaaatccg gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca
    24721 tccaatcgaa ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta
    24781 cagtcatcct ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga
    24841 gctagcttac gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac
    24901 aacgacaaca acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg
    24961 tttagcgggt aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga
    25021 taaaattgtg atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca
    25081 tatagcggtg attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga
    25141 atatgtcaaa gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct
    25201 taactggaat cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc
    25261 tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta
    25321 taaaaaagaa aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga
    25381 aaatgcacta gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg
    25441 agaaacgttg cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca
    25501 aggcagaaaa ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct
    25561 aagtggattt gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac
    25621 atttgatgaa ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac
    25681 aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga
    25741 ttctgtagaa caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt
    25801 attatcacca gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag
    25861 attcatggaa gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga
    25921 aaattttggt tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca
    25981 attatgtaaa gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc
    26041 tagaaaacaa aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat
    26101 ttaaacgaaa aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc
    26161 gtcaagcaaa aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat
    26221 atgtttccgg agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg
    26281 aacaaaacag tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg
    26341 ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta
    26401 atcctcgagc acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt
    26461 tggatccaga aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca
    26521 atcaagcagt atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg
    26581 cttcaggaga tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata
    26641 ttagtgtcca gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg
    26701 tggggcgtgc tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt
    26761 tggttcccag cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc
    26821 acgcaggagc agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga
    26881 cagtcaacga aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag
    26941 aacaaggaat ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa
    27001 aataaaggcg gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc
    27061 ttctgatgaa aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca
    27121 agagatgaaa aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga
    27181 ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa
    27241 aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt
    27301 tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc
    27361 tttgatcatc actgtattgg gtataccgct gatttttttg atctattatg tttctcctta
    27421 tagcaaatta caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat
    27481 ctctgataca aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac
    27541 ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa
    27601 agggatgaat gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa
    27661 aaacaatcaa tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa
    27721 tcctacaaaa aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt
    27781 aacaaagcaa tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta
    27841 cacgccaaga gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt
    27901 tattgtaaat atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga
    27961 tatggatgaa aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag
    28021 cactcaagaa acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc
    28081 ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc
    28141 gtctagcaca aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga
    28201 taatccaagt acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta
    28261 agaatgtact ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta
    28321 ttaataagta tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag
    28381 gagggaatcc cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca
    28441 tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga
    28501 aacgcaaaat cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa
    28561 gcgatacaac gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta
    28621 agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt
    28681 aacggagatt caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt
    28741 gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc
    28801 gatgggttcg agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac
    28861 ggtctgttgt ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat
    28921 gccggattaa tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt
    28981 tctgacggag aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca
    29041 acggctgtta tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa
    29101 tttgtcacta aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg
    29161 aaaataaact acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta
    29221 gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt
    29281 tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca
    29341 ttagagttgc caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg
    29401 gacttggcac aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg
    29461 ctgagaaacc cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt
    29521 gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg
    29581 gtagttgaac aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc
    29641 tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc
    29701 tttacaaata tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga
    29761 taataatatc aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa
    29821 tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac
    29881 agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata
    29941 tactcgtggt ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag
    30001 tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat
    30061 gggtggagga accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg
    30121 cgcattgact gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg
    30181 ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc
    30241 aaataaccgt ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga
    30301 agcagataat gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg
    30361 ttacgttaac cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt
    30421 gatggggatc ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat
    30481 ctcctctcca ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac
    30541 aggcggtttg gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc
    30601 agcaactggc gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga
    30661 aatccgtgtg acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag
    30721 ccgaccagct agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga
    30781 tcaagctaaa ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg
    30841 tgaacaaaat gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga
    30901 atttgataca ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc
    30961 accgtttttc cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc
    31021 agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag
    31081 ttacattagt agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag
    31141 ctggcgttat gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt
    31201 tatttgattt tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa
    31261 aattgattgt aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag
    31321 aaatccagaa gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg
    31381 aggaatcaaa acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg
    31441 cctgcacaaa aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg
    31501 agcaaatcca taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag
    31561 gttatgccca tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg
    31621 cgattgaaga aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa
    31681 ggaggaacaa aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct
    31741 gatgaggaag agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca
    31801 actaaaaaaa atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat
    31861 gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa
    31921 aagaaaaaag taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca
    31981 gcttcaagaa cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa
    32041 ccaagagctt attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc
    32101 ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca
    32161 ggaacagttt atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt
    32221 acaccgactg ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg
    32281 tttgatctgt aagaggagga actacgattt atactattgc actattactt catttacttc
    32341 aacgggcagc ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc
    32401 caggcgggta tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca
    32461 gtttgttcga tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa
    32521 tcctgatctt gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc
    32581 atttagttta ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa
    32641 ttttggatgc aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg
    32701 taggcgattg gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg
    32761 acccaagaca atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca
    32821 tgttttatgg cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt
    32881 acgaacctgt agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat
    32941 ttgccagcct aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct
    33001 cttactttgg cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg
    33061 tggatcattt tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac
    33121 caatcaaata cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag
    33181 ttagttccat gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt
    33241 ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg
    33301 attttgtact tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc
    33361 aagaacttga ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta
    33421 aataaaataa aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac
    33481 attcccgaca aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt
    33541 tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca
    33601 ttctgaagaa aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat
    33661 cgtagctcaa gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat
    33721 cgttacttct gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca
    33781 tcaattaaca actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa
    33841 agcaagacag cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca
    33901 acgtatcagc ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga
    33961 aattttacaa ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt
    34021 agcaaaacag cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga
    34081 agcacctatg acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa
    34141 tgaaaacgag tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga
    34201 agttcggtaa gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt
    34261 agagtaagaa gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg
    34321 caactaaatt tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg
    34381 tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt
    34441 aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa
    34501 agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag
    34561 ctttgttctt catgatggac ctccatatgc caatggtaat atccatttag gacattcatt
    34621 gaataaaatc agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc
    34681 atatgttcca ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg
    34741 cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact
    34801 ttcccaagta gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga
    34861 acatccttat gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa
    34921 aatggctgaa aaaggctata tttacaaagg attgaaacca atttactggt ctccgtcaag
    34981 tgagtcttct ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt
    35041 tgtagctttc aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat
    35101 ttggacgaca acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt
    35161 tacgtatgta ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga
    35221 aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca
    35281 agaaatggaa tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt
    35341 aggcgatcat gttactttag acgctggtac tggattagtc catactgctc caggacatgg
    35401 ggaagatgac tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc
    35461 aaaaggattg tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa
    35521 tcctatgatc actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac
    35581 tcatagttat cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca
    35641 atggtttgca tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga
    35701 gtgggttatc ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg
    35761 ggtaatttcc cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg
    35821 agaagcaatc atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg
    35881 atcaattatc tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc
    35941 aggttctcct aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc
    36001 tggttcttct catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat
    36061 gtatttagaa ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt
    36121 ggctatcaat ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg
    36181 tgaaggccgt aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa
    36241 acaatttggt gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt
    36301 tcgtgtttct atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac
    36361 gatgcgtttc ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta
    36421 caatgattta cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga
    36481 aatccgagaa gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa
    36541 cttcttgacg gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat
    36601 tgaagcagaa gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt
    36661 agctttgaca aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt
    36721 cctgaaagaa gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc
    36781 aaaccaagaa gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt
    36841 aaaagcattg gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact
    36901 aactgtttat ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc
    36961 acaattattg atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc
    37021 agataacgct atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac
    37081 ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct
    37141 atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt
    37201 cgaagaaaag taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt
    37261 atcaatcgtt taattttcca cgttcacgat accagttatc tggttgccag atccattcga
    37321 atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt
    37381 tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag
    37441 attgagcgac ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata
    37501 gtaatttttc gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca
    37561 atttgattgg ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa
    37621 atccttcagt tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac
    37681 atggttcatc gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga
    37741 tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc
    37801 aacggaagtt atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat
    37861 ccactttgtc ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat
    37921 attgcccgcg aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca
    37981 atgctttgat cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg
    38041 caagtaatgc tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat
    38101 gatcgtagta gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat
    38161 tgtcgatata gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg
    38221 agatgttttg gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg
    38281 ggaatacttc tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg
    38341 gtttttctat gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat
    38401 gagaaacgat cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt
    38461 ttaattgata gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg
    38521 aatcatttac atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat
    38581 ctgaaggatt caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc
    38641 agggacggcg agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa
    38701 ggcggaacaa agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg
    38761 taaacaatac atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa
    38821 aatcgtgctg gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat
    38881 cggtccttca taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat
    38941 agtgactaag taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt
    39001 accgacagga taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt
    39061 aggaatcgat cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc
    39121 tgcaagatgt tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca
    39181 gctatagtct aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact
    39241 gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac
    39301 aagctcttct ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag
    39361 actagatacg atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat
    39421 tttcaaataa tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca
    39481 tcattagtaa gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag
    39541 gatttttaga cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt
    39601 ttataaagga tatatatata cattgggagg gatctatttt tcagataaca acttatatga
    39661 agtgtctgca gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc
    39721 atactgatca cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga
    39781 ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct
    39841 atcactaaag aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt
    39901 tttctttttc aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat
    39961 catttttttt atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt
    40021 cttctttatt tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt
    40081 ttttactgtt tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt
    40141 tgaagtatca gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac
    40201 attgtatgaa aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa
    40261 aagaggtcct gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga
    40321 aaaccgccta tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc
    40381 aattgcctaa tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat
    40441 taactggtcc atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata
    40501 tttatttaga gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg
    40561 aaaataaagc gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag
    40621 gagaattaac gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt
    40681 tcgaacatct tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg
    40741 ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag
    40801 atgaggtaac tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta
    40861 tacaatagaa agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta
    40921 ctcatcaatt tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt
    40981 ctagacattt ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg
    41041 caacagattt gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc
    41101 cgaatagtct tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag
    41161 ttgaaaagcc agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg
    41221 aacacaatgt gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga
    41281 ttgccgatta cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat
    41341 attcttcgcg ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt
    41401 tctcaggtgg tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt
    41461 ttggaatgcc aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc
    41521 ttggatgggg gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg
    41581 gtgattctta tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag
    41641 tcaatgcaat tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg
    41701 cggtaacagc tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg
    41761 ctcatccgaa agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa
    41821 atgtgaacca agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata
    41881 atgaggaaga tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa
    41941 atatgaggat aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact
    42001 aagtaataaa caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta
    42061 tctttggcct ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc
    42121 ttcttctcaa gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa
    42181 agagttgaaa gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt
    42241 gaaggaaaaa ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc
    42301 aaaaaaaata aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt
    42361 tgacgctggg aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca
    42421 gctagctttt ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg
    42481 gaaaacgttt gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca
    42541 ttacttttta cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat
    42601 agaagatttt catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga
    42661 aaaattgctg tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact
    42721 ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga
    42781 tgtggcggct agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc
    42841 gttaagtgaa gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg
    42901 aatagtaatt acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc
    42961 atcaaatata acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc
    43021 aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa
    43081 ggagcagaac ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa
    43141 aaaagagaga aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa
    43201 aacgggaaca aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc
    43261 ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta
    43321 actatcgatc gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt
    43381 gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat
    43441 gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat
    43501 tctttaaaag tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg
    43561 catacgatta tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat
    43621 cattttgcaa tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt
    43681 gcggattatg ctcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg
    43741 gcttacacag gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata
    43801 ttgtttttag atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc
    43861 attgataaag gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt
    43921 cagatcattg gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg
    43981 attcctatgg caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa
    44041 atcatctctc tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa
    44101 aaagaagcga ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa
    44161 cgagacttga aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa
    44221 aagctaatca ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc
    44281 aaggaaatac cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt
    44341 gaaccaggat ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat
    44401 aacgatattg aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta
    44461 gagaccctca tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact
    44521 aaaagttcga ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg
    44581 tatgatgtgg ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt
    44641 gctttgcatc tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca
    44701 gatttgaata acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca
    44761 acgatcattg aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt
    44821 atgtttcttt ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt
    44881 ctcatattga tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta
    44941 ttaggaacaa aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg
    45001 tatgaagaag tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc
    45061 ttgttaacag atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc
    45121 attcggacga aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg
    45181 gcaagtgttg gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg
    45241 atcgtacgtg atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta
    45301 atcgtgacat gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca
    45361 gtgattaatc aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc
    45421 aaaaaacaca tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt
    45481 gatgttgatt atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa
    45541 ctaaatattt taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca
    45601 cttgctggat cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag
    45661 acagttcagc agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga
    45721 atcgttatcc atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa
    45781 tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca
    45841 aacttgatgg cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt
    45901 cttacacaaa tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta
    45961 cacactaaac atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt
    46021 cagctctttt ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg
    46081 agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag
    46141 tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt
    46201 tgcggtctct gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt
    46261 attaggtcct caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg
    46321 tattccatta gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct
    46381 tgcacaagca gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg
    46441 gaagatcaac aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag
    46501 agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg
    46561 aaaatcgctg atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg
    46621 acacaagaag cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat
    46681 cacttaatga catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt
    46741 cgcgtataca gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac
    46801 ggatttttta ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt
    46861 aaaaaatgaa cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa
    46921 tcggagcgca aaaacattta gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa
    46981 caatggcggg atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt
    47041 ttaacagtac ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg
    47101 tcatcatcgg aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct
    47161 ttgccttttc ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg
    47221 ggatcgttgg tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag
    47281 cattagatgt gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg
    47341 caacgattgc agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt
    47401 tgaatggaaa cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt
    47461 gtaaattgat gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat
    47521 ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca
    47581 attatatcgt aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata
    47641 ttgcagaacc attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat
    47701 ttgttcaagt attctggttc tttggtattc atggaccaaa tgtattggca ccagtgctag
    47761 agggaatctg gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg
    47821 gaaaaactgg tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg
    47881 tccgcggatc gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac
    47941 tgatagctat cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca
    48001 ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga
    48061 atgcaatcat gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga
    48121 cagctactta tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc
    48181 cgttcttatt gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag
    48241 tatgtatggt cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg
    48301 accctgccgc aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg
    48361 ttcctcataa tgaattttct aggaggaact gaaatgatta caattgccag tgcaatgcaa
    48421 caagatgaaa tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt
    48481 aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg
    48541 gctaaaaaag caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa
    48601 tcaacagctg tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt
    48661 ttatttttat gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa
    48721 aggtatgatt tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc
    48781 ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga
    48841 aaaaattaaa tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg
    48901 atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg
    48961 tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc
    49021 agtcttataa gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca
    49081 aaccattgga cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca
    49141 tgcttcaaaa gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac
    49201 gtttcaaaaa aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact
    49261 atttagttat tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt
    49321 cctgcgaatc ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag
    49381 agagctaggt gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg
    49441 ggcagataca cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta
    49501 cggatttttt gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt
    49561 gaatttttac ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga
    49621 agcaagggga caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga
    49681 tcgtaaagta agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg
    49741 ctatgaacaa tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat
    49801 caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag
    49861 aagaatttat gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg
    49921 gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa
    49981 tgaggaagca acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga
    50041 gtggggattt ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa
    50101 tggtgctttt gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct
    50161 attagaaaat ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga
    50221 tatttctttg tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca
    50281 aatagaagaa gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa
    50341 tggaaaaaat catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat
    50401 ccaacatatc acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga
    50461 aagaatacag gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca
    50521 gccaattctt aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac
    50581 ccttttgttt ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta
    50641 aatttgaatt ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct
    50701 tctttcgtaa tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt
    50761 tcttttggct tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt
    50821 ttatcatctg ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag
    50881 cagataagct tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg
    50941 tagctcagta ggatagagca atcgcctcct aagcgatagg tcgctggttc aattccagtc
    51001 aggaacgtat aaataacccc tgtatccttt gcggtacagg ggtttgtttt gtatttgtgc
    51061 caaaaatgtg ccaaactagt tatcattttt atataaacta tcgaacacag tattgatttt
    51121 ttctttatcc tgatcttcaa gctctcgaac aatatgaaga taagtagaca ttgtagtttc
    51181 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
    51241 aatagatgca tgtgtatgcc ttagtccatg caaagtaaat tgcttttcaa tccctaattc
    51301 ttttaatttt ttgcgcaaat atttgctgac ggcgtttgat gaaactaatc cattttttaa
    51361 gttgaaaaaa acaaaattat gagggttttt gatttcaaaa ttctcgaata attctttttg
    51421 attgatttta aactttttta agagatcaat cgtatgttta tcaatggaaa ttttcctatt
    51481 tgaagtttcg ttttttgttt ttccccattc atttaattta taattccatg ttttattaat
    51541 agtgatgatt tgatcttcga aatcgatatc ttcccatgtt aatcctagaa gttcagcaaa
    51601 tcgcattcca gtggcaccag caactaacac gagcatagga gaagagtact gcgcagaaag
    51661 gttttcttct acgagtttca ttaaagattt aaattgatca taatctaaat atttatcttc
    51721 ttttggcttc aatgaatctt ttcctttgat tactgctttt ctagttggat caaacggaat
    51781 taatccttct tctacggcat ctttcaacga cgctctaata tggttattaa acttaataac
    51841 ggtagatttt acatggtctt tagcatactt atttaaaaag cgttgatatc ccactctatc
    51901 taaatcagaa atcaataccg ctggcatata tttctttatg ttcattaacg tatcttcgta
    51961 ttttctataa gtgataggag atactgttcc ttctttataa agttgcatcc aatcctcaaa
    52021 gtaatctgat aataatagat ttttgcgatc ccctttaagg cctttgttca agttatattc
    52081 taattcgttg gcggcatctt ttgcttctcc ttttgtctta aatcctgatt ttcttatctt
    52141 cgtgtatttt ccgtcatctt ttttgtatga aatttcgtat tgccaactat ttccacgttt
    52201 gactaatctt gccataattg aatcagctct ctttctctga tacaatagga actataaaga
    52261 agcccattgt ttgggtttta ttattttttg cacatccttt ttgcttgccg gcgcaaggat
    52321 gtgctatttg tcgttttaat ggtataattg gatgtgtatt agatcatcca atcagaggtg
    52381 tttattatga ttaaagtgat ttttaaaaga ataactgaac ttccagagtt tagattattt
    52441 gttgattatt ggtatgtctg gttttttatt gttgcattgc tgataggtct agatgtcata
    52501 ttagaaagaa aacttaagta agagttgagt taataaattg aattcctaaa tgagatattt
    52561 tgtagcccat attttgtttg ccgacatcac ctaatactat ggagctaatc gttttttcta
    52621 acagaccaaa atcagataat ctatttaagc taccatctat tagctcttgt gaatagccag
    52681 acatgctgat atttccagtg aaattttcgt ttccacgact cattaagcat gctaacaatt
    52741 gaatctcaat cgatgttaat tgattcagta caagaagata atactcctcg ttatcccatg
    52801 aagcgctgtt aattcgaagt aaacaattct tataaagatt tttatacata tctattttat
    52861 tttgtgcctt tgatttttct acctcgtcat ttatcccttc gaatattcca atgagttggt
    52921 ccttgttctc taagttgaaa acatcttctg gaatttgatc gcgaatgttt tctaatgatt
    52981 cgtttaaatt tttgtagaat ttctcgatgc gtttgaaacg cttctcgttt ttagacccaa
    53041 aataaagtga ctgcacaacg ccaccaatat aaggaattgc tccaataccg ccttcaacta
    53101 ttaattcaag atattctttt ttggttagtt tttcattgct attatccata gttacctcct
    53161 attttgtcat ttcctttaca agcattgtga ctcctgatgg cactcttttg tataggtttt
    53221 attattgtgc acaatctaac tcttagtggg gcgtggttca tttatttttt acttacttta
    53281 ctattaatca tatcccattg agcataggat ttttctatgt tcgatcctct tgaactaatt
    53341 tctcctgtaa aagttacttt atcgcccttt gtaaaggtat cagcattgtt taagtccttc
    53401 gcaaaaatta catatgaaac tttaggtgtt gcagaaatat cattccaact tttatcatcg
    53461 tactctttgt ctgctataat ggctattcta gtagtcattg attcgataac agttccttca
    53521 aaagtatatg ttttaccact aatatattta gtaaaagctg tattttgctc agtagctgga
    53581 gtaatagaat aataactatc taagaaagca ccccagtcat gattatttgt ctctaaaaat
    53641 tcttcaaatt gagttaggct tttattgctg ctgttagatg tagtagtttg agtatcagat
    53701 gatgagcttt cctgtttgtt attgtcattt gcctcgtttg atgaggaaag cgagaaacct
    53761 acggtagtaa aaataatccc gataataagg agaatcatag gtgttttctt atttgttttt
    53821 ttgataaatc ttacaattaa catgattaaa cctataataa ataaaacaat accagtaaaa
    53881 ccaaatatac cgtccattaa tagatcctct ttctcttttg atattgaata ttataaacca
    53941 agcaattctt ttttcttaaa atcgaattct tcttgattaa taatcccttc atctaacaac
    54001 tctttatact ttttaatttc atctgcactt gaaatttgtg tttctttgga attattatct
    54061 aatatagatt gttgcttctc tatatatttt tttaaatttt ccatttcatt ccaatatttt
    54121 tttgtaaaca taacggtgtt ttcatctttc gtggccgcta atatacctcc gcgactttcg
    54181 tttccgccta gcaaggtaaa ttgtatataa ccattactca tacctggttt ttttaattgt
    54241 acggctgata tatttttaaa ggggatagtt ttttctcctt taataccttg attaactaaa
    54301 tttataaatc cttttctaat aattgaaata gaatcatcag ttactttaat caaggttttt
    54361 cctggagatt ttattaaaat ctctttttcc attttttctt cctcatttct gtgatatgat
    54421 tttttttgta gaagctcata tatgaggaaa gagcccgtgt tgcagcacgg gcttttttaa
    54481 tgttttgctg aaatcggttt ttttaataat tcttgatgta ttcatacata ttgccttgcg
    54541 taagaatatt ttttcttaaa atagcattgg cagacagcat aacaagtgta tctgagctta
    54601 ttctgaatag aataatactc cataaatttt tcgagattaa actgagattc atcagtcagt
    54661 tcgttctcaa tatagatatt taataaaatt agaatagcta ttttatctgc ttctgtttca
    54721 aattttgagt gaaaagttgt agaagtatcg tacaaaactg aaaattcaaa atgagaggcg
    54781 ctgaaatgtg caagttcatg agataaatga aaggcttctg cagtttcacc gtatagattt
    54841 tcattcaaaa aaatgattct gggttttgga tagtagaaac caggctcttt catctccata
    54901 taaactactt tcaaattgta ttcgctcaac atttctttca atttcaaata catacaaacc
    54961 atcactccaa ctattcattt tcctctaaag ctttagcaat tgcaatcgct ttacgcattg
    55021 tctccttaga tatttctttt ccatcaaaag aaaaaacagt atcgtcttct gataaatcca
    55081 catgtttagg ggtttctctt tcttctctac ctagaaggta gtctacagag acatcgaaat
    55141 agtcagcaat ccgacttaat tcatctgaat taggagtatt atttttccac tttgctagat
    55201 aaccgttaga atatccaaaa ttaatttcta actgccgtat agatatcttt ttcctcttcg
    55261 ccaagtcttt tattatttca taggtattca tcgataaaac aacctttcta aatgtttaca
    55321 aaaaaagttt agaaaaatac gcagaaataa tttgactaat tctgagtaat gagctatact
    55381 atatcttgta aacagattta acaactaaaa agacaacaaa aaacactatt gatatgtaaa
    55441 tgcagaccgc caagaaagct ttaaaatcaa tgtttttatg tcttatttaa ttatgctctt
    55501 agtatagagt attactcaga tactgtcaat tgaatttaga aaaaagttgt taaatttgtt
    55561 tacgaatata aaagaaagga atgagaaata tgaacacacc gcaaattttt aatttcgaac
    55621 aaaacgaagt tcggacaatt ttagtaaatg atgaaccata ttttgtaggc aaagacgttg
    55681 cgagtgtttt gggttattca aacactaaag atgctttgtc gcggcatgta gatttggaag
    55741 ataagatggg gtcgcgaatc acgacctcag gtcaatcaag agagatgaca atcatcaacg
    55801 aatctggttt gtacagttta atcttaaaat caaaacttcc ctctgccaaa aaatttaaac
    55861 gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca tggaggttat ctaactccag
    55921 aaaaagtaga agaagctttg cttaatccag atacaatcat tcaattagca actcaactaa
    55981 aagaagaaag aactggaaga ttaatcgcag aacaaaagat tgcagagtac gaacctaaaa
    56041 tctcctattt agatagcata ttatcttcta cagattcagt aacaattagt cagattgcag
    56101 cagattatgg gatgtctcca caacagatga ataaattact tcataaacta ggtattcaga
    56161 aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat gaaccaagga tacacaaaat
    56221 ctcatacaac tgagatcccg aaagccgatg gtggcactaa aattgtaatg aataccaaat
    56281 ggacacagaa agggcgtcta tttatctacg aattactaaa aaaagaagga tattaccctc
    56341 aaatggattt agaggaaatt ggttagaaag gagttttagt atgactgaca ttgcagaaat
    56401 cactcaacga gatagagaaa aaatcaaaga atatgtcgaa agttcaaagt tcttaactta
    56461 caccatgctt gctgaaagat ttggaattag caaaagctac ttatctttaa ttttaaacgg
    56521 taaaaagact tctgcagaag caaacagaat tatagattcg attatcacta tgtacgaatt
    56581 gtaaagggag gaacagctaa tgcaatatct agaagcaaaa atcccaattc cagaaggcta
    56641 tgtaattatc tcccaagtgg attatgagga gttaaaaaaa gctgatgata ctggtagatg
    56701 gatgacgttg ccagaagtgc tagaacggat taacagaaaa tatgattggt ttacttctag
    56761 agttttaaag aacccaagat atagaaatat tatcgatatc gagaaaaaca aaaatggatt
    56821 tgtctattat ccagttgaag gaagagacac atatctattt ttaagaagta aaacacttga
    56881 atttttagaa acaaattttt cggaaatctt aaggaggcaa gcggatggga aaatttaata
    56941 gagcgctagt gttcagcgca ccgctaatca tctacgcttt aggactttgg ggaagcaggc
    57001 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg
    57061 aagctgagta cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga
    57121 gaaactagaa tgtatattac tattactcag tttaattctt tcactaattt ctctattggg
    57181 aagtttgtat ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc
    57241 atttttgttt ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg
    57301 taacaagata tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta
    57361 ttatcatcga ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg
    57421 cggtttaagt tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag
    57481 ttagaaatga ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca
    57541 acttctagtt gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata
    57601 aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc
    57661 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
    57721 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa
    57781 acatcaacat gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca
    57841 gatgtttgta gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat
    57901 tagtagagat ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt
    57961 caaacaaaaa tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt
    58021 tcctcaaaca attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa
    58081 agatgtttcc atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa
    58141 gcaaaaagga aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga
    58201 agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat
    58261 cccaagatat ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat
    58321 gtggttgcca gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact
    58381 ttttggtgat gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt
    58441 gctggtggaa atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag
    58501 catggcaaca aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat
    58561 cataaggaga ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga
    58621 tcctgattgg ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct
    58681 tttgacgaat gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg
    58741 ggatcatgtt tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt
    58801 catcgaagat ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat
    58861 ctcttattac ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg
    58921 aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca
    58981 agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga
    59041 atgatccaca ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa
    59101 ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt
    59161 atgtatctgt ctcagtaacg gttaaaggac acacggaaac cgaattattt cctgttttag
    59221 attatcgaaa ccaaccagtt gttaaaccaa atgcaatgca aatcaataac tcattgaagc
    59281 gatgctttgt gaaagcattg gctctacacg gactgggatt atatgtattt caaggggaag
    59341 atattccaac accacctaga atcgatacaa agaaattaaa catgctagag acgattctag
    59401 aagctttcaa tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg
    59461 agcagacaga taaattaggg ctcttagctg ataacgttga aactattgaa cagttaagct
    59521 atgagcaatg tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa ttagataaga
    59581 agtgatatga gtgtttaaac cattaatcga ttcatattca gcggttctga aaaagttcaa
    59641 aggaaaagac ataagcgcaa ccatcaatga ggaagtgaac attgatcgac taaagacgat
    59701 gtatgacggc tacgatggtg atcgagtcat tgaaattcgt tttattgatc ctagacgttt
    59761 caccgtacag caacgaaact tcatctatgc gctgataggc gatattttta tcgatacagg
    59821 catgccaacg gacttctgga aggaattctt ctactttcgt tttgaaggtg tcacagggcg
    59881 caaaataagc ctgaaagacg aatcgaatac aactgtgagt gatgccaatg tcttagcaaa
    59941 tatcatctta gatttcatct ttgaacatca tattcctttc aaagaaggct atgagatttt
    60001 acctgcgaat caagaatatt acttctacaa atgcatcaca aaaagagtct gctgcatctg
    60061 tggcaaaaca ggagctgaca tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa
    60121 agaagttgat catgcagagt acacatttgc agcactctgc agaatccatc acacagagaa
    60181 gcacaaaata ggtgtgatta atttcaaaaa taaatatcaa atcaaaggga tcaaattaaa
    60241 ccagaaaaca atcaaaaagt taaatattgg agggtaaaaa tgacagaaca tcgaagttat
    60301 tatgcgatta taccagccaa cgtaaggtac gacaaaagac ttaaaccaaa tactaagttg
    60361 ttatacggag agataacggc cttgtgtaat gaaagaggct tttgttgggc aggcaatgag
    60421 tactttgcag atttatatgg tgtgaataaa gagaccatat cgcgatgggt aagtgatttg
    60481 attaagtttg gatacttgaa tcgggaaatc atttacaaag agggtaccaa tcaaataatc
    60541 aataggtacc tacgaattaa tcaataccct attgacgaaa aacgcaatac ccctattgac
    60601 gaaaaagtca aagataataa tacatctatt aataatacat ttaataatac aaaagaatat
    60661 ataagagagt taccgccttc gaaaaaatcg aaggctaagc ccgtccgtca taaatacgga
    60721 gagtataaaa atgttctttt gtcagatgag cagatggaga aactcaaaac agaattccct
    60781 aatgattacc aagagcgaat cgaacgactg tcagagtatt gtgaatcatc tggtaagact
    60841 tataaaaact atttggcaac tattcgaagt tgggcaagga aagaaaaaag tgaacctaag
    60901 aacgcaagca gtggatacaa gcgcacagga agacgagaga agcttcctga atgggcaatc
    60961 gaccaagaag cctatctcaa gaaaaaagcg ctagaacgag ctaatagaca atcaaaagca
    61021 ccattctaag aggtggaaaa atgaagatcg attatctaga actaattaat gaaatagcga
    61081 attataaaaa gggcgaggaa ttagacgtcc tgagagacgt atatgatcaa ctcgaagaag
    61141 ctggaattga aggaattaag aatgatcgtt cgagttggag taaactcaga tactatttcg
    61201 cactctatat cgatggaacg caattaagaa atttagcata taccaaatta ctattcattg
    61261 attgtgttaa aggcttgcaa aaacatctta atgaacttga gcaggtgtaa acaagatgga
    61321 cctaaagaca tttacagcac agatcgaatt aatgcatcaa gaagctttaa gacaaagtgt
    61381 gtcgtacgaa gacaagtggc tcaacacatt tcatggcgga cgtgagagcg cacttgatca
    61441 agtactcaaa ttactgaaag gagaatgtcg ggatggataa gaaagcggca atgcagcgaa
    61501 ttatcgaatt gacttattca gaagattggc aggaagataa aaaaatagtt gcagaagtcc
    61561 aaaagctcgg taaatcaatg tggactgaaa agcccaaacg gagaacgccg agaaagattg
    61621 caatctggca tggtgatcga attctagtaa cgggtaccgc taaacagtta tctgaaatta
    61681 ctggtctgag caaaaatatt atttgggata gagctaggag cttatggatt gattcaaaag
    61741 gaagacagtt taggtatgtg gaggagaaat aatggacgaa ctaatcgcga aagtagagca
    61801 gtgggcaaaa gataagggat tggatcaagc ggatttcagc aagcaaatgt taaaaacggt
    61861 cgaagaaatc ggggaagttg ccgcttctct agctagaaaa gatgaacatg gtttaagaga
    61921 cggaattggc gatgtagtag taaccttgat catcttggca atgcaaaatg atatggattt
    61981 atacgagtgc ttggattttg cttatgacga aatcaaaggt cggactggaa agatggtcaa
    62041 tggtgttttc gttaagtcca gtgacctgaa ataattccat taatggaaga aataactgaa
    62101 taggaggata gacatgattg atgcaaaaca agtgcaaaaa cagaaagatg gaatgttgat
    62161 gtttgaagca tatgttctac catttcttaa tcaatttgaa gttttggaat gctctgccag
    62221 cggagaagaa ttggagtatg ttgtaattcg tgaaaccaaa gaaaacgttc agaagctaaa
    62281 cgaatttctt tgcactatta actgttggga tatgatagct ccaggatttt tatgtccagc
    62341 tatgggagaa tttttggaat attgtcgtct tgaagatgca ggcactttgg atttagctta
    62401 tctggtatac aactacctaa acatcaacac agatcatttg tggtttggca ctgccgaaag
    62461 aaaatgggta gtccgctaac caccaatatc aatgaaaaaa ggcggtttcc tgaaagtgga
    62521 attgccgcca ttgctgtaag gttctttacg cgactcaagg tcacagaaat ataaatgcaa
    62581 atctcttttg aagtgatcca aagctctcgg aaaatggaag ttttttgttt caaagaataa
    62641 atgagatttg cgtcaagaac ctgagagtta tttccaaaaa agcttccaaa gaaccgaatg
    62701 agctaaggac atcatgtgta aacacataat atagctctcc tttcacggct aatcataaca
    62761 tgaaaaatat tcgatgtcag taggaaaaaa ggtgaattat gaaaagaata ttaaattatc
    62821 ccggcagtaa cagattctgc aattaaactc cgaagctgat gtatcagtat caactgtaga
    62881 atattatgag cgtggagaaa gtcaagcagt cacaaatgta aaaatcgata acgtcaacgg
    62941 tcttatctgt ttagatgaag gcgatagtat gaatgaaatt gctgcagacc atgaattatc
    63001 agaaactgct ttaattgatc aaatcgaatt agctagacaa tactttgttc catatgcaga
    63061 tacctggtcc aagcataaga atgacatcat ttttccagag gaacaatgag agcaactgat
    63121 ccagtaatta tccttgagga agccaaattt atttggactc acgaagagat agagcaagca
    63181 cgcttgctct tttctcaagg agttaagccg agcaaagtag ctgaaataat gggtcaaaag
    63241 attcttgatg tcggattgct tttgctccat ctagcagaaa aaaatttgat ttgaggtgaa
    63301 aatgatgatt caacttgcag gcatccaaac agggaaaatt tatttttctg gagaaagcaa
    63361 aagcgaagcc aaccaatggc tattgaaaac ttacacgaac aataagaagc taaggaaaaa
    63421 atatcctgat ttgttgctaa aggatgatca gattatgccg gaaccgatga ttttgacccg
    63481 taaggaggga agttgaaact aaaaaaccag ccgtgtgggc tggagttgct atttcttttt
    63541 aataatgagt ttttctaaat ttaattcatt ctcacgagtt tgcgtccaat aatcagaata
    63601 atattttaat tgtttaagag taggaatgtc gtcttcggta aaattagaaa ataattctgg
    63661 agaattatct ttttttaatt gttctaatcc tgattcttta aaacgttgtt caacgagttt
    63721 tactaaattg ttgatattca tgataaatca cctttttaaa tttaatttcg atttgattaa
    63781 tcgatacaag tattatatca aataaaaaag ctgatttagg gggtcttaca aaaacactaa
    63841 aattttttca aaaagaggag gaacggtggt gaaagtcaga gaacaattca agatagtgag
    63901 cgacgaagat aatgtttaca aaataaagat cgttaatatt aatgaatatc gagaagcaga
    63961 tcacaaatat ttggttgact tatacatgaa cgataaatat ttgggagaag gattttgtgg
    64021 agattcactt tttgatgatg agcgaatttt gaaggaggaa acagaatgat ccacgaacta
    64081 aaaatactac cagaatattt tgaagcagtg acgagcggac gcaaacaatt cgagatccgc
    64141 aagaatgatc gtgacttcaa agttggtgat cagttaatac tacgtgaata taaaaaatac
    64201 attaaacatg aattacctgg actaacgaga ccaacattta gttacactgg tgactcatac
    64261 aaagcagaga tcacttatat tactgattac atgcagaagt ccggatatgt agtgttgggg
    64321 attaaagggg tgaattaaat gaaagtatac atcttagcta tcacagaggg cacatggatg
    64381 ttcccagtcg gttccggaaa aatctataaa tcaaaaacag ctgcttataa agcatttgaa
    64441 aaatacaaaa aagaaaacgg tggcggcact aacgcaaaga ttctagtggc tgataactgg
    64501 catgaagaag gtgaacgtaa ttgatttacc tgttcatttt cgtggtacta actattttta
    64561 tgctagtaat cttattcaaa aaaagatgag taaagacgaa cgaaaaattc tggccaactt
    64621 ttatttagta ttaaaaatcg aacaaaatgt gttttcggaa aggagaattg tatgggaaaa
    64681 acaaaatcaa agattaagaa gaaaaaacga cgtttggaac aaaaggcaat ccagaatgga
    64741 acggctaaga aaaaataaaa aagctacccc tttcgagata gcagcgcaat actattctac
    64801 cataaggggt ggcgtttgtg agatttcatt ggttaaaaga ttaccaagaa ctagatgagc
    64861 agattcttta cttaaagtgg aatcttaata aaagtaagct tgaattgaat cgatgggtct
    64921 gtggtgattt agcaaacgtc cgcatcgaaa agaattcgag aacttcttct ttagaagaga
    64981 atatccaaaa gatagaaaat gaattggaat tgttgatcga gcaaaaagaa gaaatgttat
    65041 tgctgataga tagcttttct ggtatcgata atcaaattgt taagatgaaa tatgttgatc
    65101 aaatgagctt agaagatatt gctgaatctg ttgggtatag ctcatcgtat gtcagacagc
    65161 gacatgcaga aattagaaag acattgaact ttttagatga gtatgaacac agacaagctg
    65221 accgattgaa aaaagaaaac gaaattgatt tttacaatag cgagaagtac aaagaacaac
    65281 tctctttatt ctaaaattac actgttcact aaatgttcgg tcattgtagc tatgtaaaca
    65341 ttgttagcag catgatattc tattagtgtc aaaaaaatat gaaagagcca agatatccca
    65401 accgttttat taattggtat ctgtggctct tttattcttt taattagtag ttggcataaa
    65461 gttatttcca tttataaatt gatcagctaa atcagcatca taatcagccg cgataaattg
    65521 cgctgctgat ttaataaatg catccatatc atgaacatct ttattagtat gtctacgaac
    65581 atagtgcgtt tcatcattac caatccaagt agcagctttg gctaatcttt ggagtttagg
    65641 aaaatcattc aaataatttt caattgtttt acctaataac atttttttta ttttatcttc
    65701 atcctctgaa tttttagata ttgcataatc tttaattaga aattctagag cttttctata
    65761 accaactcca gcaatttgtt ccaattgctc agatttagct tgtgtggctt gagtataaat
    65821 atcaacaaat gtaggagaaa ctttttcaat attttctggt aaatctattt taatcggcgg
    65881 acggtaataa tctttaatta aaatattttc agaatatcct tcatcttcat taaacgtatt
    65941 tataaattgt aaactataaa atttgttgca ctcatcattt atacactgta ctaatacgcc
    66001 aaagattcta tcattatcga acgcactata tgatgaaacc ccaaaataca catacggagt
    66061 cattatattt ccgcaatgcg gacaactatt tggcaaataa attgtctctg tattttgacg
    66121 atcacgatac ttaatattaa cttcttgaag cattaaaaca cctcgcatct tttttataaa
    66181 tcataccaca aaatatctga taggagagta aacaaatgaa tgattttcat gaagcggtac
    66241 ttagtattga agtagaatca agtttggcta aagtttataa gaaagcaatt gaagcagaga
    66301 atagtccgta cagagaaaat tggaatggta atcatgcaca tgtgcaagtg gagaatgatg
    66361 attacagaac tggaatgaat acgttagtta tttctttatt gtcacacact ctacccaatc
    66421 tgcaggaaac gattgaatgg tatgagcgaa tgggcgcaaa agtaatccgt acaaattaca
    66481 aaggagagaa ttaaaatggc aatgatcaaa attagaacat ccattagtgg aacagagtat
    66541 tgggattcag aaaagaaaag gacagtagtt gttccgaaag atcaagaacc aaattttgaa
    66601 gtaaaagaag aaaaaggaat tttgagtgat ggcaaatcat ttgttgctgt caatggcgaa
    66661 ctcatagctc atagcagtga aattcttgat agcgatggaa acactgctgc taattttgat
    66721 ggagacgaag ctactaatga tcagtccact gaagatacgg atgaactgga caatatgact
    66781 gcaaaagaat tgcgtgcata tgctaagaaa catggtattg atattcctgg tgctatccgt
    66841 gcaaaaggcg atatcataaa tcttattcgt gaagtagaat gaaatactgt cagtttgacg
    66901 gatgcacgaa caaaatagca aagggtatct actgtactga acacaagaga tcaagcagat
    66961 cacgcaagaa gaagcagcaa gcaaagtctg tttatcatca tgagaacaaa ccattctatc
    67021 gaacgcaagc atggaaagat atgcgtcaat ttatttatga aagagaaggt ggtcattgtc
    67081 agcggtgtgg tcagttcata tttggaaaga gagcacacgt ccatcacatt gtaccaatca
    67141 aagacaacga actgcttaag cttgatccaa acaatctcat gctattatgt tcaaaatgtc
    67201 atccaattgt tgaaaacgaa acggaagaca aaaaagtttt tccttcgtat ttcaattgaa
    67261 gcccccctat tcattttcaa aattttttcg cgtggggaga tagggtagcg gggagtcacg
    67321 cgcatcgtta ggtcaaattt ttcaaaaaac aaaggggggt gtatacaaaa atgacgacta
    67381 aagcgcaacg taaagcgatt attgatgaaa aagtaagtgc tgaaaaagct cgtatcttag
    67441 aaataatgaa tttgtctgat ttgtacacca tcactcttga tccattaatc gaatcatact
    67501 tggatatttt tgaaatttac caacacaaat atttattgtg gaaggaaaaa ggatttccgg
    67561 agacgcaaaa attcacgaat aaatcaggag ctactaatca atcaaaacat ccattggcac
    67621 aacaagtaga aacttgggca gataagaaga tgaaagctct agatttatta ggattaacaa
    67681 acaaagcaaa aactggtaga ctaattgctg gaggatcgac cgcaagaaaa gatgaagaaa
    67741 ttacacgccc agaagtaaag ccagtagatg aactagcagc gcacagaaat aaatggcgta
    67801 agaaggcagg tgctgaaaaa tgattgaacc tggtgtaaat tatgctgatt tatttgcaaa
    67861 agaagtaaga aagaaaccta agaagtatcc taagaccgtt cgtttagcaa tagatcgctg
    67921 gtatcagtgg aaaaaaagaa aagatatttg gtttgatgta gatcgcgcga atgaaatgat
    67981 ggattgggta gaatcattta ttgttcacac aaaaggagaa atggtgggaa aaccgtttct
    68041 tttagaaccc tgggaaaaat ttatttattc ttggatttat ggttgggtta aagaaaatga
    68101 aaaaggccaa attgtacgcg ttactcgtga ggcttatgtc cagattccaa aaaagaatgg
    68161 taaaacattg attgctgtcg gttcattagg atatgcaatg tacggagaag gggcgttatc
    68221 agttgattgc tatgcttgcg catctgattt tgctcaagcg caatatgctg ccaaaccttt
    68281 tgcagttaca atcttaaaca atccaatcct acttgaagga actaaaatat ttagggggcc
    68341 aaagggaact gtttctagta ttacttatga ctatattcat gaagatatgg cttactcaaa
    68401 taaatttatt gttcaaacca agaatattga taatatcgag ggatccaatc catattttgt
    68461 attgaacgat gaattgcata aacaggagaa aatggaacag tatgacaatt ttaagtctgc
    68521 tcagatatct ttaccacagc cattgatgtt taatatctca actgctggga aaggttcgtc
    68581 gtctgttgga atgcgtgtat atcgcgaagc aaaagaagtg ttgaaacgtg atgataatga
    68641 ttcaaacttt gttctaatct atgagccaaa taaaaattat gattggacgg atagaaaagt
    68701 ctgggaaatg tgcaatccta actggggaat atcagtcgat ttatctgctt tagaatcggc
    68761 atttaaaaca gcgcaacggt cagctcattc caaagctgaa tttctaacga aacacttgga
    68821 tgtatttgtg aacggcgcgg ataatttctt tgaacaggat caagtagagc catgtttagt
    68881 taccacacaa gaacttggtg atttaagtgg cgaaccatgt tatatcggtt tagatttatc
    68941 acgtacacga gatttgacct gtgtatcttt aaacttccca acatgggatg aagatggtaa
    69001 agcggttctt aaagtaaagc aactttattt tattccgaat gaagatttag agtttcgaga
    69061 aaaagaagat aatgttcctt acagtgattt ggctgaacaa ggtttcgttg aattttgcga
    69121 cggaaaaatg attgatcaag atcagatttt gcagtacata gaggactgca tgaatttata
    69181 tgatattcag caagtgaatt atgatccagc gatgagtgac aaacttgttg agaaattaga
    69241 aaacttagga ttggaatgcg ttgaagttgc tcaatatcct aaagtattga atgctccttt
    69301 tgacgatgtc gaacggttgt tttatgagaa acgaattcaa tttgataatc cattattcct
    69361 ttattgcact ttgaacgttg tagcaattac taacatcaat ggacaaaaag cgccaagtaa
    69421 acgtcaatca aagaaaaaga ttgatgggtt cgtggcgttt ttgtgtggtc ataaggaaac
    69481 gatgaatcaa atgacagata ttgattcaga tgagttagat gattacctaa gttccatcta
    69541 tagataaata gaaaggcggt gaaaaaaatt gaaattacgt gatcggttat cgaatgcagt
    69601 ctattctttt atggaaaaac gtggatatat cgaggatatt tttggacact acacacgtta
    69661 tggtcaaaga tatgtgacgg attcatctat catggaatct tctgatgttt atgaattagt
    69721 tcaagacata tctaatcagg tggcattggc cacaccaatt gttattggtc ccgacggcaa
    69781 cgaagtcaaa gatcatcact tactaaatat tttgaagagt cccaatgatt atttgacagg
    69841 attcgaattt accaaattgg aaacaaatac tttattgatc aatggtgaga cattcccatt
    69901 aacggatagg gatcagcttc atttggcgta cggtgtaaca actaaaatca atgaacgact
    69961 tcaagaagaa tttgaaatga atggtcaaaa aatacctggt caaatgattc gacacattaa
    70021 gaacatcgga actgattcat taaaaggtgc tggaataatt gatcttgcaa gaaacactct
    70081 agaaggcgtt ctgagcgctg aaaaagtttt gacggacaaa tatactaaag gcggtttact
    70141 tgcgttcatg cttaaactag acgcccacat caatccaaat aatagcgccc aaacaaaaat
    70201 tgtcaaagct atattggatc aactggaagg aacgcaaaat gagagtgatc attctgttaa
    70261 gatgattcct ttgggaaaag gatattccat cgagacatta aaaagtcctg ttgatgacgc
    70321 ggcaattttg aattatttag gtgtttacaa aaaagactta ggaaaatttc taggaatcaa
    70381 tgttgatacg tatcaatcgc tgatgaagac agatattgaa aaagcgatga tgtatctgca
    70441 caacaaagca atcaaaccaa tattgaaaaa caagagcgaa cattacaccg ctcttttttt
    70501 tatgcctaat tctggctata gagtggaatg gaaaattaat attttggatt ttgttcctta
    70561 ctcaacaaaa acaaatattg gctacaacat cgttcgtacc gggattacaa gtccagataa
    70621 cgtggcagaa atgcttggtt ttcctaaaca gaatactcca gaaacacaag ctatctatat
    70681 ttcaaatgac ttatctagga ttggccagaa aaatgcaaca gatgattcct taccaacgaa
    70741 tgatcaagac ttgaaaggag gtgatggaaa tgaagaagaa ggaaattcgc acgattgaca
    70801 tcaccaatct ttcaacacgt tccgatgaag aaactcatac gaggaccatt agcggctacg
    70861 ctgctgtatt caacagtcaa acacttttat gggatgactt aagcgaagtg attgttcctg
    70921 gtgcattctc aaaggcaatt agtaattcgg atgttcgttg tttgttcaat cacgattggt
    70981 ccaatgtgct aggacgtact aaaagcggaa cccttcgcct tgaagaagat gaacgtggtt
    71041 tgaaattcga agttgattta ccggacacaa cggtagcaag ggacttagtt aaatctatgg
    71101 aacgaggaga catcaaccaa tgtagttttg gttttgtgcc gactgaagaa acgtgggact
    71161 acaattctga accaatgctt cgaacaatcc atgaagtgga attatacgag gtttctattg
    71221 tgcctttgcc agcatacgaa gatacagaag ctgcattaag aagtcgtgat gaattagaaa
    71281 aaaacgtcga agaaagaaaa aaattaatca aaaaaattaa tcaagcgcta gaagcgtagg
    71341 aggaaaaata catggataaa gaattattga aaaaaatgaa ggcacgtcgt gagcaacgat
    71401 tgactgaatt acgtgaaaaa gttgaatcag gagaattacg cgaagcagat ttagaagccg
    71461 tgaaagaaga aattgacagt gttattgatg aattgaacgg aattaaagac gaattaggcg
    71521 cagattctgg aactgatgaa acagacgaca atacagatga tcaatcgaat agtacggatt
    71581 cagacgaaag ccgttctggt gaagacaacg atcaagaaga agattcagac agtgaagata
    71641 gttcagaaaa tcgttctgga atgattactc aacagcaacg agatggatta cttggatcaa
    71701 ttaagaacgg attggaggca cgtgcaaaaa tgaccaagaa acaaaaagat caacaactac
    71761 gaaaagcatt tgctaatttt gtagctggaa atatttctga agcagaagct cgagctttag
    71821 ggattgaagc tggcaacggt tcagttactg tcccagaagt aattgcatct gaagttatta
    71881 cttatgctca agaagaaaat ttacttcgta aatacggaac agtggtgcga acatcaggag
    71941 atgtcaaata tccaattctt gtgaagaaag cagatgcgaa tgtaaacaag aaagagcgtt
    72001 caactgatat tgctgaaaca gctattcagt ttgatgaaat tttgcttaat cctgccgaat
    72061 tcgatgcttt ggcaacagta actaaaaaat tattaaaaat gtctggtgtt ccagttgaag
    72121 atattgttgt ggaagaattg aaaaaagctt atgtgcgtaa agaaatcaat tatatgttca
    72181 atggtgatga cgctggaaat gaaaatcctg gtgcattagc caaaaaggct gtagcattta
    72241 aaaaaccttt agatctaact gcttcaggtg ctgggcaaaa attatatgat gcattaatcg
    72301 aatttaaaaa tacaccagtg acagaagtga tgaaaaaggg acgctttatt attaatcgag
    72361 ctgctttgac tgccattgaa aaaatgaaaa cagatgatgg atttcctttg ttgcggccat
    72421 tcacacaagc agaaggtgga attggttacc aattagttgg ctatcctgtg gattggacag
    72481 atgcagcaga taaaaagggt gaaccagaca cgccagtttt atattttggc gatttttctg
    72541 catttaaaat tcaagaagtt attggtgcct tggaaattca aaaacttgtt gaaaaattct
    72601 ctggtaaaaa tcaaattgga ttccaaattt acaacttgct agatggtcaa ttggtttatt
    72661 ctccatttga accggcagta tatcgctacg aaattacaaa accagttggt ggttaagatg
    72721 gaagagcaaa ctaaagaatt gtctttagag gaaaaattca aatcacatat tcattttgaa
    72781 gagggcatgg atgattcttt gctctctttt tatttaaata tggcaaaaga ttatgtcaaa
    72841 acagcaactg gtggccaaca agaatatctt attttgatgg ttgccggcat tgcctatgaa
    72901 tatagagttt cagaggatga actcgacaaa gctatgaatg ccatgacgcc atttatcgtg
    72961 caaggagcga ttcaaaatgc cgaagagaca gaccaataat ctcagatgga aagccgaatt
    73021 gctagacatc aaaacaggaa cagacggaaa cgatcgtcca actacaattt acgaatttaa
    73081 gcgtctaata ttctatgaag aactcggtgt gacttctcaa gaaaaatatt tatcacagca
    73141 agccaagaca gacgttgtca gacgaattaa agtcagatgg gataaatcca tcacagagaa
    73201 attaagtgcg ctcaaaattg attctgtaac gtataacatt actcgcattt atacgaatcc
    73261 cgatgcaaga gaaatggagt tgagtttagc ttatgtcgat tagctttgat gaattgaaaa
    73321 cagcgctgaa atcaacaaag ttagcagtgt tcagagacaa agccagatta gggacgatgt
    73381 atccatacat tgtgtactca aatgtgagta acagcaaaaa aatggcatcc ggtaaagtct
    73441 ataaaaaatt accttattac caagtctctt tttttacact tggaacagaa caggatttag
    73501 ttgttattga acaagcgctg caaaatgctg gcattccata ttcagatttt acaggtatac
    73561 aaggtgatga gaacgacgat accgtaacca actattacac atatgtgagg tgtgtggaaa
    73621 atgccaagta ataagaatgg tttttctgaa atatcggatt atttaggaaa cctttcaaaa
    73681 gttgatccga aaaaattatc gttggaatct ttagaagaag cagctaactt ttatctaaag
    73741 cagttgcttc ctaatattcc taagtcgctc ttaaaaaaga aacatatgcg cgatcaaata
    73801 aaagtagtag ttgaaaacga tcaagtgaaa gttcaatttg aagagacggc cttttactgg
    73861 cgctttgccg aaaatggcac aacaaatcaa cgtgcccaac attttgcaag tggtacgtat
    73921 gaacaaaaca aagaaaaaat tgaagaaatt atgactagaa aaatacttga tttatgggaa
    73981 gggtgatttt aattgggaaa acaagatact ttttattttg aaggattgga cgacatttta
    74041 attgcgatga tggcaacgcc agattctgtt ggaactgcac cgacttatag tgaagtttta
    74101 cgattgccaa ttgccacaaa aatcggtgtc aaagggaatg gaacagcatt agaaaaatgg
    74161 gcatccagca aaatgtttcg tcgtgtgtcg cgagaaacaa aacatgaact tgcgttggat
    74221 cacgtgggta ttccgataga agtgatggat gaaatcaaag gattagttgc taaaagcgga
    74281 gtaacgttta gcaaaaatac cgcacgagaa tttccgtact tcgcctttgg atttattgga
    74341 aatattgaaa atggcggtaa aaaagcagtt tggtatccta atacacaact atccaatgtg
    74401 attgacgaag agtatgccac tgcagaggaa gaaacaaaaa ttgacgatgt aactgcaaat
    74461 ctggtttcca tcggactgaa atacaataat gtgatgtatg caagttttga ttcaaataga
    74521 gacggagctt ctctagaatt atttaataaa ttcattgctc aacctgtata cgatgaagaa
    74581 cagtggaaaa cattggctaa agttggaggt gcgggttaat ggctcggtta tctgattatg
    74641 gaattcacgt tgaagactta aaaaattctg ctgttgtaat tattcaaggc gcagaattcc
    74701 ctatttcatt tactatgcaa acgatggaat ttatagcaga tgtgtatggt ggagattatt
    74761 cgcaatttga atctgatatg aacgccatgc tatctaaaaa agaaggacaa atttcttctg
    74821 ctaacttatc gcctagtgat ctaaaaatca tgcgtgcctt gatttatgcc atgctgcgta
    74881 ctggtggttt agaggaagat ccagaaacta ttttcaaatt tttgggaatg agtggagagg
    74941 tgttgtctgc ttatagtacc tgtatggaaa tttttgctag ccagacattt caggtggaag
    75001 acctaaaaaa atccaagaag ccacaagact ttcaaaaagc gcaagcaaaa agaaagaaaa
    75061 acaaaaagaa tcggaagaga taggaactcc ttggagtttt tatatttacg ttgctctcac
    75121 tctattgaat tggagtgaga gtttcttttt aaagtctaca cctaacttgt ggctcaaatc
    75181 ttatttacag tggttacaac aaaacaccga ttttgaacca cctcaatctg taactatgga
    75241 taaatcgcct tggtggtaga aaggagcgct aagataaatg gctggtaaag aatctgatgt
    75301 cgttcttaat tttaaaacga atggcgaagt cagttattcg aaaacaatca aagaaatcaa
    75361 caaagaaatg aacttagccg ctgccgagta caaaaaccaa gtgtctgcga tggacaagga
    75421 tgcaactcaa acagaaaaat tgcgagcggc taaacaaaaa ttagaaaaac aattaggatt
    75481 ggctgaacaa cgatctcaaa tgctgagaga ggaatatgaa aaatctgtca aagaaaccgg
    75541 tgaatattct gctgaatcag aaaaacttta taaacaatta ctcaattctg aaaccggaga
    75601 aaataaactt cgtacagcat tagaacagac gaacgatgca cttaaagaac aaggtgacgt
    75661 ttctgttgat acagcaaaaa aactccagaa aatcgaagaa acaggtgaga aagtaaaagg
    75721 cgttggtgaa aaaatgtctg ttggagtaac cgcgcctatt gtagcggcag gagcagcagg
    75781 acttgcagca tttggtgaag ttgacgaggc acttgatacc atcattacaa aaaccggagc
    75841 aacaggtgat caagctgata gactttcaca gtctttcaaa aacgttggtt caaatactca
    75901 tttaccttta caaacggttg gggaagctat tggtgaggta aatacacaat ttggattcat
    75961 ggataaaaaa ctggaagatt caaccaatta tctcctacag tacgctgaaa tcaatgatac
    76021 agatgtttcg caatcagcaa tatctgctcg acaagctatt gatgcttatg gactagaata
    76081 tgatgatttg aattctgtcc ttgatgtaac aacgaaaaca tcgcagaata ctggtcaatc
    76141 tgtagacgac ttgatgcaaa aagcaattga tggcgcacca caaattaaac aattagggtt
    76201 gagctttggt gaaggggtca ctttacttgg acaatttgag caatctggtg ttgattcaag
    76261 cgcagcttta agtagtttat ctaaagcaac agtggcttat gcgaaagacg gaaaatcttt
    76321 aagtcaagga ctagaagaac tacaagacaa ggtaaaaaat gcaggttctg aaacagaagc
    76381 catcaatgca gcagcagaag tattcgggac taaaggcggt cctagaatgg ccgatgcaat
    76441 tcgtagaggt actttgaacc tggaagattt agcaaaaact gctggagaaa gtgggggagc
    76501 tgtaggagac acattcgatg ctactctcga cccaattgat caagccgatc aagctatgaa
    76561 caatgcaaaa ttagcaatgg ctgacgtagg tgaagcagtc caagtaagcc ttttgccttt
    76621 ttttgaaaaa gcaacttcct tattacagga attctctaaa tggtgggggt ctctagatca
    76681 agatacaaag aattggatca taacaattgc tggtatagcg gcagcagttg gaccagtact
    76741 aatcgttctt ggttcactta tgggatctgt tacaaaaatc gttggggaag ttaaaagttt
    76801 tataggagtt tggcaaggac ttgctggttt atttggaatg tcgggaggct ggtttgcatt
    76861 agcagttata gctattggcg cattagttac tggactaatt tgggcatata ataacgttaa
    76921 atggtttcat gatggagtga attctttctt caaaggtgtt tcagatgtag ctgttgaaat
    76981 atttaatttt ttaagtggtt attttggtgg atttttcgat ggtgttatag caaatttcaa
    77041 taatttttat aatgctggaa aacgaatttt cagtggtttt ctagatttta tcactggagt
    77101 atttacaggg aattggtcaa aagcatggca aggtctagtt gatatttttg gaggtatttt
    77161 cgatggtatt gttgctgtag ggaaagctcc tattaatgcc atgattggat tgatcaatgg
    77221 atttattggt ggattgaata atataaagat accaaaatgg gttcctggtg taggtggaaa
    77281 atcattttct atttcaaaac tgccttattt agcacaaggc ggtcatctaa tcaatggaca
    77341 agcgattgtt ggtgaagctg gacctgagtt actgactgcc aagaatggta aaaccacagt
    77401 tactccatta tcagacgaag aaaaacgcaa agggatcagt ggaagagttt ctggaggtag
    77461 cattgaacag catatacaca ttggcaaagt cgatgcaaac aatccatctg agttagatcg
    77521 tatgaatcgt aaatttgcta aagcaaacaa acaggctatt cgtgatttgg gaggtgttcc
    77581 tatttgagtc gacaatttat gaatccagat gaaccaaatt ttatttggaa aaatcggaat
    77641 gcagtaattg atatgaattg catcattgag gcagagcttc cagatattat gccgaacaag
    77701 cgatacgaaa cttacacgat tcagggccga agcggagaat tgacggagac gtttgatgac
    77761 tatgaacctt ttgatttgga aatagaaggg atcactattc cacattcgaa actgagagaa
    77821 gtcaaacgat ggctcactgg taaaagccaa ttaatcacac ataatgatcc agacaagtat
    77881 ctagaggcta tctgcagcat ggataaagaa gtccctttcg aaaacgagtg gggcttcttt
    77941 tatacattcg atgttacttt tcgttgtcag ccactcaaaa gaaaaatcgg tgaagcacct
    78001 aaaatttttc attctagtgt tatggatatt tttgatcatg gtgacgaaat tgcacatccc
    78061 tatctggaaa tagaatcgaa cggaggagat ataacgatcg ctgtcggtga taaaagccta
    78121 acaatactta atacgttagc tgcaacaatc gtggttgata ctcagcttgg aaaagcaata
    78181 caagaaggtt tgaatttatt tacgcgtggt gattggccag tattgcagcc tgaatggaat
    78241 caggtaaaaa tatccggaaa atttaaagaa gtgcggtttt ggaataggag tgtgtatctg
    78301 tgacgcaaga atttatttat gcttataaaa aaatgccaga tgatttgagc gtgaacggag
    78361 cgtcgttagt tgattgggaa gatttgccag aaatcaatcg tgtgctgaat ggccaatatc
    78421 gtttttatgg taactattca agaagcggac agtatcgctc ttatttaaaa aaaggaaatt
    78481 ttataaaagc gaaggtacca gatggatcat ggcaatattt tgaaatttat aatgtcaaaa
    78541 agaatttgac ctccgtttct gtaacagctc ggcatattgg ttttatggcc aataagaact
    78601 ttattgtcaa gtcgtttacg gataacggca atgggtctca gatcatgacc aatcttaaaa
    78661 acagtttggc atttgatcaa aaatttaact atctttcgaa tgtagggaca acgcaccaat
    78721 ttacagcaag acaagtcgcg ccagttgaag caatcattgg ttcaaataac ggtaatcaaa
    78781 atttgactgg cgtgactagc gcggaacttg atatggataa ctatgatttg aaactggtaa
    78841 aacagattgg atcagataat ggttttcgga ttgattttgg catcaattta gaagcaatcg
    78901 aagaagaaat tgatgaagaa tcaattgtca atagtttgta tttggttggc ggtgttcctg
    78961 ataacgatta tgatgaagac aaagaaccta tcgaatatgg ttatttagaa attgatggag
    79021 taaccaacga gaatcgacgt attgccaaac gtgaaaacgg agattgtaaa acagttgatg
    79081 aattgatcaa atggggcaaa accttatttg acaatgatcg tatccatgag ccaaaagcta
    79141 ctcatacagt cagtatggtg gcgttggaac acacactcga atacggtgac atgtatcaag
    79201 agctagcgtc tttacacttt ggtgatgtcg ctcatgttag ggcgaaagag ctagatatcg
    79261 agataaaaga gcgtatggtc gaatacactt atttcccaac gcttgggaaa tataaggata
    79321 ttgttttagg taatgattta tcactgtaca cttcggcagt aaatactcaa gcacaagaat
    79381 tgaaaaagaa aatcgacaat cgaacagaaa cgttagttca aaatgtgttg aatgcgacgg
    79441 catggatcac tggtaattct ggtgggcatg tcgtttttcg tccagaaaaa gctccatcag
    79501 agattcttat catggatact ccaaatgtgg cgaatgcaaa gcgagtgtgg cgttggaatt
    79561 taaatggctt aggttattca gacaacggag taaatggccc gttcggtatt gctatcacat
    79621 caaaaggaga aattgttgct gacttcatca aagtaggaac aataaatgca gaagtattcg
    79681 aaacttcctt taatgcttat ggtgatgtgt taaaacttgt aaaaggcacg cttcaaattt
    79741 ggaatgaaaa caaaaaaatc atggagctaa ccaaaaaagg tatggaattc tggaatagaa
    79801 aagaatccat tggaacgatc ggaacaacag attcagcagg aaatccattt ccaaatgctg
    79861 taacacctac acctcttgag gaaaattctt tggtaatacg tacaaatggc gatggaaaat
    79921 acattttaat ttctccaact gcagaaaaag gatttgtgtt attaggaaac ggaaaagcat
    79981 attatttcgg tgatttagat attcagggaa aacttactgt tagaggtaaa gaagtgatac
    80041 caggacaaaa tggcggtcca tctggcggag gagaaacacc tggtgggtat cctgatgaat
    80101 taaaaacaga tgcagaaaag agagcttgga gaatatacga tattttgtgt aataacggat
    80161 ttacgaaaca atctgcatgt ggaatattgg ggaacattca acaagaaaca ggaggaactt
    80221 ttgatcctga tactgttcaa ataggtggac cagcatacgg attagttcag tgggatggtt
    80281 cttcatatcc tcttgttggt ccagcaacat gggatggaaa agtttatgtt caaaacttat
    80341 ttaatgctgc aggtattaaa gaaccaataa cgagcttaga tgcacaagtt cgattgctta
    80401 tttggacatt tacaaatgga caatggatgg gcgtagtaca acctacgacg gttgatggat
    80461 ttaaggcctg tactgatcca agacaagcag catatgcttt tgaacgaaac tatgaacgtc
    80521 cggcagcgac acatcctgaa cgtcaagatt atgcagttaa ctggtataac aaatttaaag
    80581 atttaaaacc aggaggagct actggagaag caggactaaa acatttagag tctttgattg
    80641 gacaacgaat tggcaatggt cagtgttatg gcttgtctgc agaatattca ggatatctgg
    80701 gtggttgtgg catgggtgct ggaacaaaat atggtttaac tcatgtgata ggaaatactt
    80761 ctgcagcatc tgatattggt attgcctatg attggtctgc tgttggttgg aaagtgattc
    80821 aaaaccctag atatgatcaa ttagtagttg gtgcaattat taattgggca agaggtggac
    80881 aagtgggttc atggtttgca gacggaactt atggacatac tggcgttatc cgaggcttag
    80941 ctaatggtcg tatgcaaact tatgaacaaa acacagagtt aggtatgatt tgtggaaagt
    81001 tagatcgcca gtattatagt gcaagtgcaa tttcttccat tgtcatacca ccaaaatagg
    81061 gaggtgattt aatggcaaag tggaatgtca tattaagtac aacagaaccc tataattacg
    81121 tggggatgat tcaagttcga caaggcaata aaaacactga agttatggaa gctacaattg
    81181 tcgaaaatgg tcttccatat gatttatcag aatgtaaggt atattttgaa tcagttgttg
    81241 gcggaaaata tccagtccaa ttagaaacaa gaattgtgga tgctaaaaaa gggaaaatca
    81301 a
//
LOCUS       DACEJZ010000003        64198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEJZ010000003 DACEJZ010000000
VERSION     DACEJZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.003"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(148..1539)
                     /locus_tag="IW656_000183"
     CDS             complement(148..1539)
                     /locus_tag="IW656_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0835917.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1752..2138)
                     /locus_tag="IW656_000184"
     CDS             complement(1752..2138)
                     /locus_tag="IW656_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0835918.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2194..2817)
                     /locus_tag="IW656_000185"
     CDS             complement(2194..2817)
                     /locus_tag="IW656_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0835919.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2881..3549)
                     /locus_tag="IW656_000186"
     CDS             complement(2881..3549)
                     /locus_tag="IW656_000186"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0835920.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3561..4001)
                     /locus_tag="IW656_000187"
     CDS             complement(3561..4001)
                     /locus_tag="IW656_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835921.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4060..4731)
                     /locus_tag="IW656_000188"
     CDS             complement(4060..4731)
                     /locus_tag="IW656_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0835922.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4916..5314)
                     /locus_tag="IW656_000189"
     CDS             complement(4916..5314)
                     /locus_tag="IW656_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0835923.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5311..6084)
                     /locus_tag="IW656_000190"
     CDS             complement(5311..6084)
                     /locus_tag="IW656_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0835924.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6081..6764)
                     /locus_tag="IW656_000191"
     CDS             complement(6081..6764)
                     /locus_tag="IW656_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0835925.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6931..7818)
                     /locus_tag="IW656_000192"
     CDS             complement(6931..7818)
                     /locus_tag="IW656_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0835926.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7815..8684)
                     /locus_tag="IW656_000193"
     CDS             complement(7815..8684)
                     /locus_tag="IW656_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0835927.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8821..10107)
                     /locus_tag="IW656_000194"
     CDS             complement(8821..10107)
                     /locus_tag="IW656_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0835928.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10380..10958
                     /locus_tag="IW656_000195"
     CDS             10380..10958
                     /locus_tag="IW656_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0835929.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10958..11887
                     /locus_tag="IW656_000196"
     CDS             10958..11887
                     /locus_tag="IW656_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0835930.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11884..13620
                     /locus_tag="IW656_000197"
     CDS             11884..13620
                     /locus_tag="IW656_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0835931.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13683..14519)
                     /gene="pheA"
                     /locus_tag="IW656_000198"
     CDS             complement(13683..14519)
                     /gene="pheA"
                     /locus_tag="IW656_000198"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0835932.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14516..15025)
                     /locus_tag="IW656_000199"
     CDS             complement(14516..15025)
                     /locus_tag="IW656_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0835933.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15018..16313)
                     /gene="aroA"
                     /locus_tag="IW656_000200"
     CDS             complement(15018..16313)
                     /gene="aroA"
                     /locus_tag="IW656_000200"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0835934.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16326..17417)
                     /locus_tag="IW656_000201"
     CDS             complement(16326..17417)
                     /locus_tag="IW656_000201"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0835935.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17434..18600)
                     /gene="aroC"
                     /locus_tag="IW656_000202"
     CDS             complement(17434..18600)
                     /gene="aroC"
                     /locus_tag="IW656_000202"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0835936.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18602..19666)
                     /gene="aroB"
                     /locus_tag="IW656_000203"
     CDS             complement(18602..19666)
                     /gene="aroB"
                     /locus_tag="IW656_000203"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0835937.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19699..20721)
                     /gene="aroF"
                     /locus_tag="IW656_000204"
     CDS             complement(19699..20721)
                     /gene="aroF"
                     /locus_tag="IW656_000204"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0835938.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21380..22354)
                     /locus_tag="IW656_000205"
     CDS             complement(21380..22354)
                     /locus_tag="IW656_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0835939.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22335..24218)
                     /locus_tag="IW656_000206"
     CDS             complement(22335..24218)
                     /locus_tag="IW656_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0835940.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24236..26461)
                     /locus_tag="IW656_000207"
     CDS             complement(24236..26461)
                     /locus_tag="IW656_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0835941.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26576..27610
                     /locus_tag="IW656_000208"
     CDS             26576..27610
                     /locus_tag="IW656_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0835942.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27677..28690)
                     /locus_tag="IW656_000209"
     CDS             complement(27677..28690)
                     /locus_tag="IW656_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0835943.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28768..28956)
                     /locus_tag="IW656_000210"
     CDS             complement(28768..28956)
                     /locus_tag="IW656_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0835944.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29396..29524)
                     /locus_tag="IW656_000211"
     CDS             complement(29396..29524)
                     /locus_tag="IW656_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0835945.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29527..30900)
                     /gene="rlmD"
                     /locus_tag="IW656_000212"
     CDS             complement(29527..30900)
                     /gene="rlmD"
                     /locus_tag="IW656_000212"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0835946.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31011..32066)
                     /locus_tag="IW656_000213"
     CDS             complement(31011..32066)
                     /locus_tag="IW656_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0835947.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32076..33506)
                     /gene="gatB"
                     /locus_tag="IW656_000214"
     CDS             complement(32076..33506)
                     /gene="gatB"
                     /locus_tag="IW656_000214"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0835948.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33503..34975)
                     /gene="gatA"
                     /locus_tag="IW656_000215"
     CDS             complement(33503..34975)
                     /gene="gatA"
                     /locus_tag="IW656_000215"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0835949.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34975..35280)
                     /gene="gatC"
                     /locus_tag="IW656_000216"
     CDS             complement(34975..35280)
                     /gene="gatC"
                     /locus_tag="IW656_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0835950.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35386..37422)
                     /gene="ligA"
                     /locus_tag="IW656_000217"
     CDS             complement(35386..37422)
                     /gene="ligA"
                     /locus_tag="IW656_000217"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0835951.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37439..39673)
                     /gene="pcrA"
                     /locus_tag="IW656_000218"
     CDS             complement(37439..39673)
                     /gene="pcrA"
                     /locus_tag="IW656_000218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0835952.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39828..41387
                     /locus_tag="IW656_000219"
     CDS             39828..41387
                     /locus_tag="IW656_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0835953.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41498..43429)
                     /locus_tag="IW656_000220"
     CDS             complement(41498..43429)
                     /locus_tag="IW656_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0835954.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43447..44361)
                     /gene="pfkB"
                     /locus_tag="IW656_000221"
     CDS             complement(43447..44361)
                     /gene="pfkB"
                     /locus_tag="IW656_000221"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0835955.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSETETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44358..45110)
                     /locus_tag="IW656_000222"
     CDS             complement(44358..45110)
                     /locus_tag="IW656_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0835956.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45269..45478)
                     /locus_tag="IW656_000223"
     CDS             complement(45269..45478)
                     /locus_tag="IW656_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835957.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45489..46817)
                     /locus_tag="IW656_000224"
     CDS             complement(45489..46817)
                     /locus_tag="IW656_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835958.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47182..47769)
                     /locus_tag="IW656_000225"
     CDS             complement(47182..47769)
                     /locus_tag="IW656_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0835959.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47852..49102)
                     /gene="clpX"
                     /locus_tag="IW656_000226"
     CDS             complement(47852..49102)
                     /gene="clpX"
                     /locus_tag="IW656_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0835960.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49349..50638)
                     /locus_tag="IW656_000227"
     CDS             complement(49349..50638)
                     /locus_tag="IW656_000227"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0835961.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50792..51724)
                     /locus_tag="IW656_000228"
     CDS             complement(50792..51724)
                     /locus_tag="IW656_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835962.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51881..52540)
                     /locus_tag="IW656_000229"
     CDS             complement(51881..52540)
                     /locus_tag="IW656_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0835963.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52543..53568)
                     /gene="sppA"
                     /locus_tag="IW656_000230"
     CDS             complement(52543..53568)
                     /gene="sppA"
                     /locus_tag="IW656_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0835964.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53581..53871)
                     /locus_tag="IW656_000231"
     CDS             complement(53581..53871)
                     /locus_tag="IW656_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835965.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54280..56085
                     /gene="glmS"
                     /locus_tag="IW656_000232"
     CDS             54280..56085
                     /gene="glmS"
                     /locus_tag="IW656_000232"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0835966.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56157..57512)
                     /locus_tag="IW656_000233"
     CDS             complement(56157..57512)
                     /locus_tag="IW656_000233"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0835967.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57536..58705)
                     /locus_tag="IW656_000234"
     CDS             complement(57536..58705)
                     /locus_tag="IW656_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835968.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58702..59589)
                     /locus_tag="IW656_000235"
     CDS             complement(58702..59589)
                     /locus_tag="IW656_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0835969.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59778..63470)
                     /gene="nifJ"
                     /locus_tag="IW656_000236"
     CDS             complement(59778..63470)
                     /gene="nifJ"
                     /locus_tag="IW656_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0835970.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63669..64190
                     /locus_tag="IW656_000237"
     CDS             63669..64190
                     /locus_tag="IW656_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0835971.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17836 a        13925 c        11458 g        20979 t
ORIGIN      
        1 cagctttttc acaacctctt attcacggtg tttcagaagt agcttctcgg aaataagccg
       61 aaatttcaca aaaatttgag aaactatttt taggaaactt cttcttattt cttaaagctg
      121 accacttcta tcacaacctc tttttgacta tgcattcaca tcgatcactt tagaatcatc
      181 attgatgaca tcgcgatcta aatctttgat gcgtgcactt gtcacgatac cagctaccat
      241 gctaccgctg acgtttactg ctgtacgtgc catatcgatt aatggttcaa cagagataac
      301 taacccaacg attgctactg gcaagttcat tgcccctaaa acgatcagtg aagcaaatgt
      361 tgcgccccca ccgactccag ctacaccaaa agaactgatt gttacaattg ccacaagcat
      421 caaaataaat tccaaactaa agacattcac tccagctgct ggtgcgacga tcgttgccaa
      481 catagctgga taaacgccag cacagccgtt ttgtccaata gacaaaccaa aacttgctga
      541 gaaattagct gttgcatcat ctactcctaa agcttttgtc tgagtctcga tattcaatgg
      601 caaagctcca gcacttgaac gtgaagtaaa cgcaaagctc aataccggaa aggctttttt
      661 gaaatagttg attggattga cttttgcacc aatcaaaatc agtgagtgga caatcaaaac
      721 agcaagcaat gctacgtaag aagcaacgac aaatttacct aaattgacga tagctgcaaa
      781 atcacttgtt gccaaaacgt ttgtcatcaa agcaaatact ccataaggtg tcaaacgcaa
      841 aacaagcgtc acgatccgca taatgatttt atacagactg tcgactaaat ctgaaaagaa
      901 tgctgcttct tttggcgctt ttcttttcac gcctagataa gcgattccga caaatgccgc
      961 gaaaataacg actccaatcg tacttgttgc acgactacct gctaaatcag caaaaacgtt
     1021 tttagggata aaggataaaa tctgctgtgg aatcgataag ttttcaactg tttcctgacg
     1081 tgttgccagt tcagagattc gtgctgtttc tgcagctcct tgtgtgaatt tagctccttg
     1141 gagtccgaac agcattgttg aaccaattcc gatcaatgct gcaatagctg ttgttccaag
     1201 taaagaagtc aatacagtcg cactaatttt ccctagcttt tcagaaactt tcatttttgt
     1261 gaatgcacca atgatcgaaa taaacactaa aggcataact agcatttgca agaatgatac
     1321 atatccttga cctacaatcc cgatccattc cattgattga gtaacgactt tgtcgtctac
     1381 accaaagatc aattgaagca gaccgccaaa gacgatacca actcctaatg ctgtaaagac
     1441 gcgtgttgaa aattttgtat ggcgcttttg catacgatag aacacatata ataatgccgc
     1501 aaaaactaga accactaaaa ctgtgatgag tgttgtcatt tttctcctcc tcatattatt
     1561 aatgacaagt ccatcgaagg aagtaaaggt tcccgagaat gattcttcaa ccatcctcct
     1621 gaaaaaattt ttttcagtga cttttatatt ttagacgatt tttatcgaaa tgacattctt
     1681 tttgcttaca attagtaata aaaaaacata cctgtgatag ctttaatcta tcgcaggtat
     1741 gtttttactg tctattcttg tttttctgat tgaaaattca attttacccc aggctgatct
     1801 aaggcgatct ctgtcacctc ataagacatt cggcgtacga tatcgaataa cggtgctact
     1861 agctcgtcca cttcttcttg tgtgacatca ttttgacctt caattttccg attgaggata
     1921 tgattgatct gactcacact tccagataaa acatatgtat caaacactaa acggaactct
     1981 aagcgtgctc ctacgatgct atgttctttc ggatagtttt cgatcgtttc ttttaaaggc
     2041 gagaagccta ctttcaaatc tgtttctact tcttggttcg gcaatctcat atcataatga
     2101 aatgcttcta caacttcttt ttgtcttctg atttccatat aattttatca ctcctttatg
     2161 aaaattataa catatctcct ttgaaagggg atactacttt ttaatgatgc tctgcaaggt
     2221 aaggatttca ttcagatttc ctaccacata atctgccggg atctcaccca gaaaatgatc
     2281 gatgtcgtaa agacaagtag cgactcctgc atttttccct gcttcgatat ctaaacgacg
     2341 atcgccgatc atcatcgtgt ctgttctttc taaatgaaaa gtatcgatca aataatttaa
     2401 agatgctgga tcaggtttac gaggaaagtc ctgatcgatc cctactactt cctcaatcag
     2461 atgtgccaat cgatgttctt ttaacaggcc ccatgttgat tctgtcaaac ggtgggtcag
     2521 gatgaaatga cgaccgccgt tgtcttttag catttccagt acttgtttcg tttctggaaa
     2581 tggacgggaa acttccgtac tttctttttc atatcggtga aataattcat taaactctct
     2641 ggcattcaag tttgattcat tgatcaaata actagtagaa tactttttca ttttgaagta
     2701 gatttctttt ttgtccatac tgataccaaa atcttttagt gcttgccatg caccatctac
     2761 cattgcggga tacgtatcaa acaatgtacc atcaaagtcc catatataat ttttcatgtt
     2821 ccttttgttc cattcctttg cttctttttt ctatctctca cttttagata aaggtgttct
     2881 ttaaagtatt tttttgtcca gaattgtcaa atcatctgct aatgtatact ggattggttg
     2941 tgcatttggg atatcgatcg tatccatttg atcttcagga acatcttcta aatattttgt
     3001 cagtgctcgg atgctattac catgacccac aactagtaaa tcttgccgat cttttagttt
     3061 tggtgctaga tgatcttgcc ataagggaat gatccgattc actgtcattt ccagactttc
     3121 tccgtatggg atcaaatgcg gatccaagtg atcatatctg cggtcaaatc tattttctgc
     3181 ggttaacggc ggaacttccg tatatcctct gcgccatgtt tttacttgat cttttccgta
     3241 tatttctttc atcttatctt tgtttttacc cacaagatcc ccataatgcc gttcatttag
     3301 acgccatgtt tttattatag gaagccataa ctgacccatt tcttctagtg cgacttgtcc
     3361 ggtaagaatc gcacgtttca aaacagaggt ataaacggca tcgaattgaa gtccggcttc
     3421 aagcattttt tgaccggcaa ctgctgcttg ttcttttcct ttttctgtta aggcgacatc
     3481 tagccatcct gtccagtaat tttcaaaatt tgcttcgctt tcgccatgac gtaataatac
     3541 taatttcatt cttttctccc ttaaacattc gtttcgaatt ttataccaca tgttttgcat
     3601 gtcacacgta ttcgtccttt tcctttaggt gcacgctgct tttgatgaca attcgggcac
     3661 tcaaaaaaag tataactttt tcgatccatt ttgacttgct ttttgttttt tatctgatga
     3721 tatttttctt tgactctatc aagatatttc aaataacgtt gattctcatt tagtctgggg
     3781 tagatttttt ttgataggaa acgaaatatc atccatccga aaaagacgat gaacaacaat
     3841 tgtccgattc tatagggcaa ccaacgactt agcaaactaa agacaagcgc agcgatcaat
     3901 agaccgcgat tcaactgatc aaatttcgca tatcttccct gcataagttt gtgatagttc
     3961 tgctgaaatc gtattagcct ttctaaccat gtttgcatca tattatctcc tccatcatct
     4021 aatatttatt atgacaaaaa aatccaaaaa gaaaaagcct taattggcct tttccattgc
     4081 aacttggtga aggacattcc gatttctggt aaagtcaatc acaatcactt tctttttatc
     4141 ttctctgttg actactcgaa atgtgtccgt caatggaaga aacagcttct tagatggtag
     4201 ctgttgcata cgtataaaac gatggatcat cgtaatcaat tgaggaaatt gaatagacgt
     4261 gtgaagcaga cctggatatt ttttcaacag ctgtgtcgtt ttgatcagat tccgtttttt
     4321 actgatcact tttaaaaagc tttttagaat cttttgctga cgtctaaaaa tagaaaaaga
     4381 attttcgtca tctatttgat aacgcatttg tttttgcagt tctgctgcag tcagatggat
     4441 cttcccatcg tttgtatcga tcattaaacc ttctggaaac agttcaacca ctgctcgatc
     4501 ccaagattca agagaaagct cgatataatc ttttactggt aattccaatt ctaccttaaa
     4561 aaagtgcaaa acagccgaca gcccttctgc ttgatagatt tctcctaatg ttagcccctc
     4621 tagatggaac tctggtgaaa aatgcagcaa tgttttattt tcctgcaatt gaacatatag
     4681 aaatgtagat cgctcaatca gagtatcttt taaaacaact aaaaaattca tttttctccc
     4741 ccttttttca tcatttttaa aatagcatat tttgaaaaga aagtctgatg cttagagtag
     4801 aatttcataa aaatttacta tttcataagt attcacgaga aaaatacaaa caaaaaagcg
     4861 gaaataacgc tcatccgcct tcttgtttgt gtttatttcc tcttcaaacg cttgtctatg
     4921 attcagactt ttctctatcg ttattttcat ctaaaagttc ttgcattccg ataatttctc
     4981 gatattgatg aatcttagtg ataccaatga ctttgtctac atcaatacta aaacaaatac
     5041 cattacctgg aatatcgatg ttgatgcctt gaccaatcgc atccattact ttattcgtca
     5101 aactatcggg tactaatgtc aacacaatat ctttttctgg cgaaatgtcc aaacctaaaa
     5161 atttccctga atcatgtaca ccagaacctc ttccatgtaa gatcgttccg cctgctgcac
     5221 ctgcttcttt tgaaaagtcg ataacatctt cgcccattcc tccgtcaacg atcgtcacaa
     5281 tcatgtgaag attcaatgct acaagatctt tcatttgctt tcccccaatt tttgcaatct
     5341 ttctttttcg attctgtctt tttcatcttt tcgtttataa atatatccta gtactaaagt
     5401 ggataatatt ggtgtcaatg caatcattgc aaccacacca aacccgtcaa tgatcggatc
     5461 tcttccttca atttgtgcag cgattgccac gctcattgat aaaataaatg tcgaacacat
     5521 cgggcccgtt gctactccac cgttatcaaa agccattgct aaaaagatct tgtccacttt
     5581 tcgtcctaaa ataaatgcta gcccgtatcc tgggatcaag aaataataaa gagagaatcc
     5641 gacgagcaaa cgccacatag acaatcctac tgctgcgccg atcccaattg aaattaccat
     5701 cagcatgatt ttcgatcgga ttctcccctc gctcaattct tcaacctgtt tgatcattac
     5761 atgaatcgcc ggctcagcaa accctactaa gaaccccatt aaaaagccaa ggggaaccag
     5821 tatataattg taaggcaatc ccgcaatagc actgcctaaa tgctgtccta ccggtacata
     5881 agccatattg actccgtgca ggaaaaggat caacccaata gtcgttatca agaaaccttt
     5941 cataatgtca aagaaacgct ttttgcgcat acgaaaacta atgatattca ttaaaacaaa
     6001 taggattacg ataggtgcca ctgccaaaat gacttccatg gtaacgattt cgattccatc
     6061 tatcacttgc tgccagaaac tcattggaag atcaccccca aaatcatcac ggcaagaatc
     6121 ggccctaacg aagcaatacc gaccattcca aaactgtcat tttcattttt cttttgtttg
     6181 atcatactag tcatccctcc ggcaagggcc aagataaatg gaacagttac tggaccagta
     6241 gtcactcccc ccgaatcaaa agcaatcggc ataaattcgg aactggtgaa aaatgaggcg
     6301 atcaaaacgc ctatgtagcc tatagccatc aattgataat aggatagctt gaatacgacc
     6361 cgaagcagcg caaaggctag aaatactcca gtgccgacgc tgacgatccc aatcagcaac
     6421 actctgccaa tctctccgtc agatatatca tatacttgtt gtcccaaaac ttgcacggaa
     6481 ggttcagcaa tcgtgatcac gatcccgata gcaaatccca aactgaccaa aacggtcaaa
     6541 cttttctttt tgatcatata tttccctact agatctccga cttccatcat agaataatct
     6601 gctccaaaca aaaagagcgt cattccgatc atcatgattg ccgcaccaac cagaaatgaa
     6661 gcgaggtctt ctccttctaa cggtgcaaaa acaaacgtta aaatgacaat caggacagtc
     6721 atcggcaata tagcagcaat cacttcttta aaatttttta gcatgtatcc tcctccttta
     6781 ctctttaagt ttaccttaaa atgaaagagt tttcgagcac aaacttaaaa cgttatcatt
     6841 taatttttct caacataaaa taacacacgt cccaaaagta tctttttact tggaaaaatg
     6901 actttgaagg aacgcgctta ttgtgttttt ttattttaca gagccgatca ttccttgaac
     6961 gaattgcttt tgcaaaacaa aaaatacgat ggcagttggt aaagtagcga tgactaatcc
     7021 agccatgatc attccatagt ctggtgtata ggaagcaccc atagcagata aaacaagcgg
     7081 aaccgtccgt ttatcaggag attgcaaagc tactaagggc cataaataat tgttccagct
     7141 actcatgaat gtgatgattg cagccgcggc ataggtattt ttagccgtcg gcatatacac
     7201 tctaaaaaaa atacttagtt cttttagacc gtctaatcga gcggcttcta ctagatcttt
     7261 tggaaaagat ttaacatttt gtcgaaagaa gaagatcaaa aaagcagtgc taacagatgg
     7321 caggatgaca accaagaaac tgttgattcc caaaaccgtt ccgttcaatt gtgaaaacat
     7381 tcgatataaa gggatcatca atgccgcaaa aggaaccatc atcgatagca gtagaatatt
     7441 gaaaattagg tcttttcgtt tactttgata aatctcaaat ccatatcctg ccattgacga
     7501 aatcagcaat gcaaacacag tggtcaccaa agcgatcatc gctgaattcc aaagggaacg
     7561 agtaaaattc agctcattgg aaaacaaatt ttgaaaattg atgatcaagt tactgccaat
     7621 ctttattttt cccgatgtga tatccacggg tgtattcgtc attcccaaaa ccatccagac
     7681 aaaagggaaa atggaaataa atgataggat gcttaatgct gtatacttta atactgaccc
     7741 aattgtcgaa cgaagttcta atgcttgttt tttgacgttg gggcgttcga ttgtttcttt
     7801 ttctaccata tttttcatcg actttctcct cctactttcg tttggattat cgacaatatc
     7861 acaataaagg aaacaataac aaaggaaact gctgccgcat atccaaaatt aggcgtgaat
     7921 ttataactca aattatagat gtattgagat agggtggtcg ttgcatttgc tggtcccccg
     7981 ccagtaatat tttgtacttc atcaaaaagc tgcaatgtcc cgatcgtcga agtaatggaa
     8041 gtaaacaaaa tgattggttt taaatttggg atcgtcactt tgataaactg ctgccattta
     8101 gaagcaccgt caatagatgc tgcttcataa atctccgaat caatattttg catgcctgaa
     8161 aggaaaaaaa tcatattgta tccagtccaa cgccaagtga tcgaaagaat gatcagaact
     8221 ctcgcccaaa ttggatgagt caaccagcca atcggtgtgt caatcatacc aatgcccaac
     8281 aacaagttgt ttacaaggcc agattgagaa aatagacttt tcatgatcag agaataactg
     8341 accatcgaag tgatacatgg aagaaagata gcggttcgaa acaacccttt atatttcaac
     8401 tttttatcat ttaaaaggtt ggaaataaaa agtgctagaa acaacatgat cggtacttgt
     8461 acaagtaaat acaggaatgt attaaaaaaa gcttttttaa aagttgcatc tgtcaacatc
     8521 cgctgataat tacctaaccc gttaaatgtc atattgacag ggggaccact ttgaaaagac
     8581 gtgatcaaag ccaaaaccat cggaataaaa accatgattg atatcaaaat gactggcaag
     8641 attaaaaaag catgcccgtt tttgaataaa tctttctgct tcattctttt ccctccaatt
     8701 ctatcattga ttatctacat gaaaagacaa gaaagacaat atctgtaagg cagacgtgat
     8761 cttttttgtc ttttcgtttg tctgatgaaa aagcgaagaa attcactttt ttaccaccga
     8821 tcaattagcc aattgtgctt ctacttgttt ttgtgtatct gctagtacct tgtctgtctc
     8881 ttctccattg atgattcgat gaagagcttc agctacgact gcttcaattg cataggtctc
     8941 atgtccgtaa ttcacttctg gtatttcaga tgtccatgtt gaaaaatcgt caaagatctt
     9001 ttgaccactg taaaattcag aagcttcttg ataagcttct tgttcattcg cgctaagcat
     9061 cgtagatact agtccgattt ctttcgcaag agtgcccatc agttcttcat tcgaagcaaa
     9121 cgttgcttct aggaaatctt tcgctgaatc ttctccagct actcctttga tcacatacca
     9181 gccagcgccg ccaaggttag aagcttgcgc tttttgcaga ttttctggaa gtgcaggtat
     9241 tggtgcaatc ttccattttc cagattggtt ttctgctcct tggatcgttg aagaatacca
     9301 agcgccagtt gggctagaag ctactgctcc tgattgtatc gcagttactc cagcattcca
     9361 gtcagaggtt tgttcgacta gatcttcttt caaaagagtg gcaaacaatt caagaccata
     9421 ctttaaagat gcattgtctt ggatcgtgac tgtctcccca tcttcagatg tataccattc
     9481 tcctgcctgt tgcataatca tccttactcg accaaggtca gaaggattga cttcagcgat
     9541 tttctttccc gtcttttctt tgacatctcg cgctacttga atatactcgt cccacttcag
     9601 attctccata tcctcttctg tataacctgc ttctgccatc agatccgtac gatagaagtt
     9661 tgctgttacg cccgaatcga aaggaacacc gtatatttta tctcctactt tgtttaccgc
     9721 aaatttatac ggtgcaaaat gatcttcctt tacaatagca gacaaattag cgaatgcatc
     9781 tggataagaa gtcaaatagc cttgtatcct ataatcttca atcaagacga tattgggcaa
     9841 tccttctgta ttcccgctcg ctaaagcggt gttcagtttc tgaacgatat catcctgaga
     9901 catcgtaaca acttctatct ctgtttcttc gttctcataa actttttttg cttcattcac
     9961 tgctttgata ttgaatgtct catcccacgc ccagacagtg actttttctt ctttgtctgc
    10021 tgtactacta gcacttccgc atcctgttaa gaaacctgat gctgccgcta aacaaacgac
    10081 tccagcaatt aactttctga tgttcatttt tatccgctcc ttttccatcc attagttgtt
    10141 tacgtttgct acattcatca taccaaaatt cgaaaacggt ttctttatta ttcagatact
    10201 tcaaaaattt ctcctaaaat aataaaaaaa tacaaatcaa ctatgacaaa aggaataatc
    10261 ttagcatcta gaccaaagat cgaaatggtg aggtttcttt aaaataaaaa tatccttgca
    10321 ttttctccta gaaataagta tttttaaatt acaagactat tcttaaggcg ggtaaaaaaa
    10381 tgccaatacg cgtaattctt atcgatgatg aacagacgat tttagatgga atgaaacagt
    10441 tgatcaattg gtctgagcac gggtttcaat taatagggac tttttccagt ccgctgaaag
    10501 cgcttgattt ttctttggta catgcaatcg atatcgtggt cacagatgta atgatgcctg
    10561 aattaaatgg aatcgaatta tcgcggctat taaaacagca acaacctgaa acacagatcc
    10621 ttatcctctc tagctatgat gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg
    10681 ctgattatct tttaaagcct cgattgagtc ctgatttttt tctaaattcc ttaactgccg
    10741 tttcacaaaa aatcaaaaaa aagcccaaca aaagcccaat caccaatcgt tatgagcaga
    10801 tctgcgagaa tctttcacag cagattgctg gacaaactcg tccattgctt gaccctcttc
    10861 tttttgaaca caccttgttt ttctgttata taccgaaaaa cggccattca gttcaaaaca
    10921 aggctctaaa caaaaacagt cgatcaccca ttcattgatg gatagcgacg attcgtttac
    10981 tatttatcct gtttctacct tttcggaaga atccggctat gtaatcaata ctgattccaa
    11041 agatacactt aggaagtttg ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt
    11101 tgttgtttct gattcttttg caatccaaga tatcaatgaa atattccaag agatcagaaa
    11161 ttgcaccaaa ggacaacgtt tctatcataa gggaaagaac cttgcttatc agcatgaact
    11221 tcttccgtta ttaaccaacc acgagcagca agcaagtagt tatttaacaa aaatcgttca
    11281 taaagactac gctttagctg tcaaagaact tgagaatttc ttcacgacga tattggaaca
    11341 gcctattgat ccagcttatt tgaaacatga ggcaatcaat cgtttctatg cattactcaa
    11401 cgctttggct gacgagcatg ttgggaatga agaactttct ttgctcaaga tcgcccttcc
    11461 ttctttatta gccgatgctg agtatttcga agatttttct attcttgtca aagaaacact
    11521 tgagaaactc acccactttt ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt
    11581 gatctacgag ttcgtccagg aaaattatga gaaagaaatc aatttacaac ttttatccga
    11641 aaaatatcat ttttcatata gctatctctc agccatcttt actgaaaaat ttggtatcaa
    11701 cttttcaaaa tatctaaaaa aagtccgtat cagtaaagcc aagctctttt tgacggaaac
    11761 acggcttact ttgacggaaa tctgtgagaa agtcggctat accgaactgg gttatttctc
    11821 cagagttttt aaagaagaga caggaatgac accttctcaa taccgtaaag gaacttttgt
    11881 aaaatgagaa aaaaaatcaa agagcatgaa ctctttgcca aacttctgtt cattttgttc
    11941 atcggtcttt ttctgcaagc catcgtcatt tctttgttta tttatcatcg atcaagggat
    12001 gcctatatcc agctgttcaa tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa
    12061 tttgaaagtt tgaatgatac aatcgagaat acattagccg cttttgattc taatccagct
    12121 gtcaaaagct atttttccaa tgatccagcc caacatatgg aacagcttca gcaactaaga
    12181 acaatccaaa aaatgaatga ctctctttca aaaatccatc caatgatcga ttacgatgta
    12241 ttgatctttg gagaaaatgg aagaactttt gtcggaaacg acatgctcac tgccgtatct
    12301 gccgattctt tttttcaatc tgcgattgct cagagagtga atgaacgtgc tgctgataca
    12361 caaatgcttt ttgcacacca cggattgact cttcgcgata agaaagcacc cagcgtcttc
    12421 tttgtcaaaa aactgaaaaa tacattgaat cacgtttacg gatatgctgt tttatcgatc
    12481 tcatccaagc aactagcgaa tctttttcaa tctgtagtaa atcctgagat atcgaagatc
    12541 tccttcatga acgaagaaca gacgatcatc gcatcaaatg aagaaaatac aattgggagc
    12601 cgatcaatga ttttcaaaaa actaacagta ggtgaaacaa cgatttcaaa tggacagcag
    12661 ttgacccgtc ttcctctgta ccgccaggat tgtacattac tcagtcaagt cgatcttcat
    12721 tctttatcta accaaatggg cgtgattttt ccgattattt tgtataatat cgtgacactt
    12781 gcttttgctg gctcattggt atttatgtat ttggatcgac acacaaaatc gatctaccgc
    12841 ttgattgatt cgctaaaacg gctagaggaa gatggcttaa aagctactgt tcctgtacaa
    12901 ggaagccatg aagtccaatt gctagggaat acgatcaatc aattgctaag taatgctcag
    12961 catcattatg aacagacgat ccaagacgaa aagaagaaac gttcgcttga aatccaaact
    13021 atgcaagcac agatacagcc ccattttatt tataatacgc tgactagctt gaaatttttg
    13081 atttggcaga aaaaaacaga tgaagcagtt caaggaatcg acagtttcat tactttacta
    13141 agaagtacga ttgggaaaaa agaagaagtg attccagtca aggaagaaat caaaagcgta
    13201 caaagttaca tcgatctttt gagtatgcga tatggcgaaa gaatctcggc taaaataatg
    13261 attcctgatg aactgcagtc attatctatg ccgaatatga tccttcaacc aattatcgaa
    13321 aacgcctatc ttcatgcttt tcacaataaa acgcaaggat tcatcacggt gtacggcaaa
    13381 acaaaaaatc agcttctttt attcgaagtg attgataacg gcgatggctt cgatactgaa
    13441 aataccaaac aaaaaaatga tcatttttca ggaatcggca tcagccatgt agatgagcgg
    13501 atacgcctaa tgtatggcga tgcatatggc ttaacgatcc aatcagtgat tgggacagga
    13561 acgactgtaa cgatttgtct accgcttctt cctaaaaatc aatcagaata tcaaaaataa
    13621 agaatagcca agcgctgatt tttttatttt aagttaaata aatcaatcag tattcttgac
    13681 tttcatctac gcgtctcttc tactgtcagc actggataac agcccaatat ttgactttct
    13741 cctccgagca tcttgatttc ttggagcgca ttctctacta atgtcattgg acggtccaac
    13801 aacaaatcga tcacgaaaaa atattctccc aagctagttt tcaatggtcg ggattcaatc
    13861 ttacttaagt tgatttcccg ccagccaaaa gctgccagca ttttatgaag catccctggc
    13921 cgattagcag gtagtgtcaa gatgacactc atttttactg gagctgactg ctggctggtc
    13981 atctttcggt caccgacgat ccaaaaacgt gtctggttca actcattatc ttggatattc
    14041 tcagctaaga tctccaatcc aacatgctgt gctgtttcta atgaagcaat cgctgctgcg
    14101 tcttctttcg gatgttctgc tacatacatt gctgcagcag ttgttgattc tgttgctact
    14161 aaaggaacat taggataatg cgtttctaaa aactgttgtg attgtgctag tgcttgtgga
    14221 tgtgaaagga tttttgtaat ctttgttgct ggattgccta aaagctgctg cttgatagga
    14281 agaacgattt cacttttaac ttcgacttct ggatgtttgg aaagaagatc gattgtgtga
    14341 tgaacagctc cttccaatga attctctaca ggaacgacag ccagatcgac ttgtttgcga
    14401 aacaaagcgc gcaaacaaac agggatggaa gcatacgcag tcaattgttc tgtaggaaat
    14461 agctgacaag ccgcctgata agtgaatgaa ctttctggcc ctagatagct gacgttcata
    14521 tgcttttgat cctttctata atggtatcga tgatcttttc tggtgtttga ttggtcgtgt
    14581 tgattcgatg tgtcgcactt tcttcatacc aagaaagtcg ttgtgccaat aatgctttta
    14641 tttcttctct tgtttttcca agagctaaag gtcgaataga cgtttcatct tgttggatac
    14701 gatcaactag acagtccgct tctgcctcta agtaaagaac aatcggctgt gtttttaaaa
    14761 aacgccgatt ttctgcactt tggacaatcc ctccaccagt cgcgataatt ttgtcttcat
    14821 ttgacagctt tcttaaaata tctgactcca cttttcgaaa gcttttttca ccgtaatagt
    14881 gaaaatattc ggcaatcgac aattggatat gttcctcaat ctttgtatct aaatctacat
    14941 actctttttg taaacattga gctaaccctt taccgattgt tgtttttcca gcgcccatga
    15001 aaccaatcag caaaatgcta gccatgagct tctcctcctg acacaagttc tgctaaatcg
    15061 tcaaaaaatg ccggataaga gattgaaaca gcttcagctc cttctagttc gcaaggtcca
    15121 tcagctatca aagcagctac ttgcagcatc ataccgattc gatgatcgcc tctgctggac
    15181 acttttgcgc catgcagagg tgtcggccca tggatgatca gaccgtctgc tgtcgctttt
    15241 atctttgctc ccattttctg taattcttct gccaccgcat caattctgtt ggtttctttt
    15301 acttttaatt cttctgcgtc tttgatgatc gtgattcctt ctgcttgagt agccactaaa
    15361 gctagaatgg gcaattcatc aatcagacgc ggaatgattt ctccaccaat cgttgctttc
    15421 ttcaaatgac tcgtcttgac acttaaatca gccgattggt tctgttcatt gtgattcgtc
    15481 tgggtaatcg ctgcgcccat ctcctccaac acatctaaga taccagttct tgttggattg
    15541 accccgacat tttttaacaa cagttggctt tccggaacaa gaagtcctgc tgctagaaag
    15601 aaagcagctg atgaaatatc acctggaaca gttatctgtt gaccagttaa tttttgcggt
    15661 ccggtcacta tgatcgtttt atcttccact gtgatccttc cgccaaattg acgaatcatt
    15721 tcttccgtat ggttacggga acgttctttt tcaacgatta cggatgtgcc ttctgcttgt
    15781 aatgccgcaa acagcaaagc agatttgact tgcgcactgg ctacaggcat attgtatcgg
    15841 ataggtgata aagagcgggt tcctgtcaca gtgattggta gatactggtc attttcttct
    15901 ccttgcaagt cagcgcccat ctcgcgtaaa ggttccatca ctcgccccat tggacgttta
    15961 gacaacgaag catccccgaa cagagtagtc gtaaaaggct gtccagccaa aatacccatc
    16021 aataaacgta tggtcgtacc ggagtttccc atgtcaattg attcggttgg ttggacaaat
    16081 gagtggctac cgcgaccatg aatcttgatc acctcttcct cttcttcgat ctcgacacct
    16141 aattgttgga aggcatgtaa agtactcaga caatcttctg cgcgcaaaaa attttgtata
    16201 gtcgtcgttc cttcagcaat tgctccaaac atgatgctgc gatgggaaat tgatttatct
    16261 gctggtatcc taacagtccc tcttaatcca tgtatttgtt gtaataattg catttatcca
    16321 gcctcctatt tgatccagca atgataactg gtattagaca taatgattct ttttgcttgt
    16381 aacaaatcct gttggttttt aaaactgatt tgcagtattc ctaaaatatc ttccctggtc
    16441 tcttggatct tcaaattaat caaagaaact cccgcttttc cgatcagtcc agttacttct
    16501 gcaataacgc caggttcatc tggcacatcg ataaataaat caaaaaaggc aggaatcgcg
    16561 ccttgcttat ggacaggcaa ctggtctcga ctttcttttg cccgattgaa aaaagaaaag
    16621 atttccgatt ggttctcagt catgatccat tctgctattt gtgtcatttc ttgttgccaa
    16681 tctgccaatc gttccaataa tatctgctta ttgctcaaca aaatatctgt ccacatgaca
    16741 ggatctgagg aagcgatcct agtgatatct cgaaagcctc ctgctgccag ttgtttggct
    16801 cgcggatact cttgattgaa ttgttctgcc tgattgacta agccagctgc cagaatatgt
    16861 ggaaaatgac ttagcattcc tgttatacgg tcatgttctc ttgctgtaag agttacatat
    16921 tttgctttgg ttccgcaaaa tagttcttgc agtttttcaa catctttttt cgtcttttcc
    16981 aaaggtgtaa agatgtaata ggcattttca aatagtttag catctgctgc ctgtatacct
    17041 gatttatgag aaccagccat tggatgtcca ccgacaaacc gaagattttt ctgctcagcg
    17101 gatgtcacga cttgctgctt cgtactgctg acatccgtaa tgatgacgtg atcagacaag
    17161 ttgattcttt caaggacatc aagataagct aaggttgtac ggacgggaac ggctaataag
    17221 ataacgtctg cttgttctgc acctgtttca aaatcacttg caacgtaatc aacgatctgc
    17281 tgttctactg cttgctcacg ggtttttgac tgactatccc aaccaactac tatcagttct
    17341 ggattggtct gacgaataca aagtgccata gaagcaccaa tcagtccaag ccccacgata
    17401 aaaacatgtt gtgccatctt atttgccccc tttttagtaa tgtttcaagt agtcacggta
    17461 ctccttcact gcagctttca actcaacaag cgaatcactt gggaattttg acaatagctc
    17521 tgtcgctacc acagtggcta caacattttc cgcaacgacg ctagctgcag gaactgctgt
    17581 actatccgaa cgttctacac ttgctttgta aggttctttt gtatcgatat caacactttg
    17641 aagtggcttg tacaatgtag gaatgggttt catgaccccg cgaatgacaa gtggttcgcc
    17701 attggtcatt ccgccttcga agccgcctag attattggta cgtcggaaat agcctctttc
    17761 ttcttcccat aggatctcat ccatcacttc tgagcctggt aatgaaccag aagtgaatcc
    17821 gaccccaaat tctacgcctt taaaggcatt gatactaacc acagcttgtg cgattttggc
    17881 atctagcttc gtgtcccatt gtacgtagct tcctaatcca gctggaacgc ctccaacgag
    17941 cacttctaca attcctccga tcgtatctcc atttttcttt gtctgatcga tcaaatcttt
    18001 tattctttgc tcaactgctg gatcaacaac tcgtacttct gacgcttcag aaatttcttt
    18061 gatttcttga atcgagatat ttccaggtat ttctgcttgg attccaccaa gacaaatcac
    18121 gtgtcctgct acgatgatgt caagttcttc cagaattttc ttggcaattg cgccgattgc
    18181 tacacgcatg gtcgtctctc ttgcagagga ccgttcaagc acattccgaa gatctcgatg
    18241 atggtatttc ataccgccta ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg
    18301 cagtgattgg tctttttcag gcacttctgc tacagacatg acagttgtcc aatttttcca
    18361 atctttattt tctaccacta aagtgacagg tgaaccaatt gttttgccgt gacggatgcc
    18421 agacgtgatc cgaacacgat ctttttcgat cttcatcctt cctcctctgc cgtaaccagc
    18481 ttgtctgcga gcgagttcgt gattgatttc ttcttctgac agaggtaatc ctgctggtag
    18541 tccttcaata atggctgtta attctggtcc atgggattct cccgcagtca tataacgcat
    18601 cttacagtcc tcctaagtaa tctttgaatg tctctgtggg aacaggaacg atttttgctt
    18661 gcccaatctt ttctagcaag atgatattca attggcttcc tcttgctttc ttatcatggg
    18721 ttatcgcgtg gaataattca tctggatgac ttagatcgta cgtaatcggc agatggaatt
    18781 tttgaagtat cctttctaaa gcagctgtcg atccagttgg agacaagccg attttttctg
    18841 catgagaagt gatggctacc attccaatag ctactccttc tccatgactg actaccccat
    18901 atcctgctgt attttcaatt gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat
    18961 ttccttggtc aaagacatct tcttccacta cttttcgttt gatttccaac gtagcagtaa
    19021 tgattggaac agcatgttcc agaagatctt tttcgtcttt cagctcgttc agctgtttcc
    19081 aaagcgttgc atctgctata gctgctgatt tgatgatttc agcgattcct tctcgtaaac
    19141 gacgtaccgg caaagttttt agaacctcag gatcaatcaa tacaccagct ggctggctaa
    19201 atgttccgac aaggtttttc gctttgtttg tattgactgc tgtcttcccg ccaatactag
    19261 aatctacttg tgcaagtagc gtagtcggaa tttgaacgaa atggatccca cgcatgaacg
    19321 tcgaggccac gaatgctgct agatcaccca ccattcctcc accaagtgca agtatcccat
    19381 cgctgcgggt aaaatcatta tctgctaaaa aatcgtataa ctcagctgct tgttctaaag
    19441 atttactggt ctcacccgcc ggtacgattg aaaggactgg ttcaaaatta gcttgtctca
    19501 attggaacat gattttttct gcatatagtg gagcaacgtt tgtatcagtg atcacagcta
    19561 ctttttgagg tctccaaatc gtttgaagcc atgatccagc ctgttctagt gcgccttttt
    19621 caatatgaat atcgtatttt tggttatcaa catgtacagt aagcattcgt ttcacttctt
    19681 tcttttaaaa agctggaatt aatcgagtgt ctgaatcttt tccattgctt ctttcatgat
    19741 cgcaacttct ttcatcatgc gtgagtaagt cttttcattc aacgattggg gaccgtctga
    19801 ccaagcattt gcaggatcag gatggatctc tacgatcatg ccatctgctc cgcttgccac
    19861 acccgcacga gccattggtg gtacaagttc ccaaattcct gtaccatgac ttggatcaac
    19921 aatcacaggg aaatgagtta attttttcat caatgggaca gcacttaaat caaacgtatt
    19981 acgtgtcgct gtttcgtaag tccgaatccc acgttcgata aaaatgacag gagatttgtc
    20041 ttcgactgca atgtattctg cagcattaag ccattcattg atcgtaccgg aaattccacg
    20101 tttcaatcca acaggtttac ctgttttacc gacagcagat agcagtttaa aattttgcat
    20161 attacgtgcg ccaatctgta ggatatcgct gtactcagcc accatatcga tatgcccttc
    20221 atccatcact tcagtaatca ctttcattcc aaattcatca gccgcttgac ggatgtattt
    20281 caatccttct tcttctaaac cttggaaagc atatggagaa gtacgtggtt tgaaggcacc
    20341 acctcgcaga atttttgcgc ccccggcttt ggccattcgc gcacattctc tgatttggtc
    20401 taatccttcg atagaacaag gaccagccat cgtcacgaaa ctgccgtcac caatttttac
    20461 tccgtctaca tccacgactg tattgccagg atggaattct cgagaagtca atttgtatgt
    20521 gtttaaaata cgcacagcat tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa
    20581 acttcgcgta tcacctttta gtccaatcac tactcgttct gttccttgat cgattgagac
    20641 tttgaatcct tctttttcga tatgatcgac gacttttttt atttgttctt ccgttgcttc
    20701 ttttttcata attactatca ttgtttttcc cgctttcttt tgtttactta tctatcttgg
    20761 ctgattttct ctactttttc aaggtggttt tacattaatt cctactaaat gaccgtgtcc
    20821 tgttgttctc attttcgctc cttctttcct acttatgacc cagataatca acaaaaaaag
    20881 atgtccctgt aggaaattgc ctctacagtg aacatcttca acgagcctta aaaagcgctg
    20941 caaaaatata aaatagccac tatagatgaa tttctctacg tggctgtaag tcgtgtttaa
    21001 aaatctttag ccatcataga tacaaacctg gtttgtttgt acccacgaag tcatgaatac
    21061 aaacctatct aaaatagcta aagtaatacg tattcagttt tgtaagtttt ctagatttca
    21121 taaaaaatcg ctccaaactg atgtaagaat tgtacctagt atagacataa aaaaatcgac
    21181 tgtcaatcat ttttatgaaa ctttttttaa ttttctgata gttaactttg tttttcagaa
    21241 aaataacaat tggttttatt gaagaaaata gtcatgaaca aaagtatctg agaacttttt
    21301 tactctcttg ttcatgacat atagaataaa gcagctttgt cttgtaaact ccgtttttca
    21361 aataataatc taaagattgc tactgaataa taaagccgat ttcatggtcc tcttcgctgc
    21421 tgattcggta aggtgactca acatccgctc cccagctatt gatacccccg actcctctta
    21481 cttttgcaaa aatcgtaaga accgttcgtc tttctggcgg tagttcttct atatgtgttg
    21541 cctgttccag ttcttctgct ttgaatggta aagcggaaaa agcaaatggc gtatcctgct
    21601 gttcaaatcg caacgaaaaa tcttcttgat ctttatccgt attatccaac gtttgatttc
    21661 ttgttagttc tacccatttc gtttccatat gcatgccgca ttcttgggga acaaggtatg
    21721 gggaaacggg gagtcctgcc actttataaa taccttcgac accaccagcc atccgatccg
    21781 gatatgtttc ccctgatagg ccttcatact caaaaaattt tgctttcgtt ggcataatga
    21841 accttaaacc caatgcgggt aattccggta atcctttttg tccataataa tgcatattga
    21901 caagcatttt accctctcca gcgatttggt aggaaattgt cacttgggtt tttggtactg
    21961 tgttgatttc cagtgtaaaa atgatttcaa ccgtttcagc gtattcatta cttgaatatt
    22021 gattatttac tggagcaatc ggcaaatcga tctttttatt gtccaccagt acttctattt
    22081 ctttgacttt tggatacaat ccggctccta accacatacc ggatttaaat ccaaatccgc
    22141 tacctctgtc attatcggtc gttgctcgcc aaaaaactgg aagtgtttcc cggtatagcc
    22201 attcttttcc gtttttattg agcgattcca gtccgcctct tgtataagaa aaaatataat
    22261 gcgtattccc cgatttgaca cctaatgtac agtcgccgaa gacgattgca actttttccg
    22321 tatccatatg acttttattt actgtatttt tcataataat atcttacctc ctgcatggcg
    22381 ggtttttctt gtccatctgc aaaaacgatc ccattccctg agaattcata gtcacttggt
    22441 ttatcgtcaa agtcaccgcc atagcgcatc acttctctgc ctgtgattgg atcagtcact
    22501 tggatcgcct gatctttaaa atcccatata tagcctcctt ggtacatttc ataacgatca
    22561 agcaacgtca tgtaagaatc cattccacct aacgaattcc ccatgtcatg catatattca
    22621 cataagatga acggtttttc cgctttattt tcaaggtagt caatgatctc ttctggtggc
    22681 gcatacatgc agctttttac atctgaaatc gaatcttcga atgctggatt tggaaaattc
    22741 ccttcataat ggacaagtcg atcggggtct acttttttga agtaatcgtt catttgtcga
    22801 atgttttctt ccgcataaga ttcatttcca agagaccaaa aaagaatact tggatggttt
    22861 tttaatgttt cgaaattcgt ttttgctcga tcaagaacag ccgcctgcca ctgaggtact
    22921 gcacctggta cgttgtaaga aggctcaaat gcgcccattt tttgccatga gccatgggat
    22981 tcgaggttcg tttccgccat cacatggatt cctgcttggt cgcataaata ataccaagtg
    23041 atctggttgg ggtaatgaga tgtccgtacg ccgttgatct ttgcattttt tatgatagcc
    23101 aaatcttttt ccatatcttc tatagtgatg gcgcgtcctt ttttcggatg ccactcatgt
    23161 cgatttaccc cgttgataat catccgtttt ccattgaagt agatgatttt atctttgatt
    23221 tcgattttac gaaaaccgaa ggaatagtca atatactccg ttactctttt ttcttcgtct
    23281 atcgtctcca attcaagctt atataaattg ggctgaaaat gcccccacag tgccacattt
    23341 tcaaatccat cgatgcgaat cgtcatttgc tcttttaacg gatattgctg ttgatagatt
    23401 tccttcccat caggatctag tatcgctaca ttcagctgac tgtttttggc tacttgctca
    23461 aaagacaaat ctacttcaag tttgccattt tgacatttgt catctaagcg aggtttgatc
    23521 caaagatctt ctaaatggtt ttgtcctcga gcaactaaga agacatctct gaagatccca
    23581 aaaaatcgga aaaagtcctg atcttccaag aatgcagctg tacttctttt gtaaacctcc
    23641 acagctagaa tattttcttc ttccaaataa ggtgttaaat caaattcaga cggagtgaac
    23701 gaatcttcgg catacccgat aaaatgacca ttcaaccaaa taaacattgc ttgttctaca
    23761 ccagcaaaat gaatcgagat ttgttttccc tttagacctt cttctaatcg aaaagtcgtc
    23821 aaatacgaac caaccggatt gtctttagca gctgaaaaga tcccttccgc ctctttctca
    23881 gggcttgtcg tgaaaggggg acggcgaaat acatgtccct cccacggata catcgtattg
    23941 atgtaatgga tcttgtcata atttgctaat tcaatatgac taggaacagt gatttcatca
    24001 aaagaacagg catcaaaatc ttttttataa aatgtttttg gtcgtaccga aggatttggc
    24061 gaataacaaa aacgccatga tccatttaaa gactgtttaa gggaggatgt ggtttctact
    24121 tcttcccttg ttgcataaca aacatgatcg ctatgtgcat ttgattggtt cactctaaaa
    24181 acttgtggat catctaacca ttttatattt gctttcatac ttttttcctt ctctctcata
    24241 actttttgaa atgaagcagt tctgtttgga aatcaccttg aaaatcgggg aagtaaagtc
    24301 caactgtcat caattcggcc cccgaaaata tccgcttcga ggtctgttcc tgataaagtg
    24361 cctcatcatc taaccccatc agatatactt catggaacgc tggttgagct gaagctagta
    24421 tcctccctaa aaacacaata gcttcatctt gtttaggaga cacaaacatc catgcggcaa
    24481 tattttcttc aaaaggactc gccaaacgat aataatggcc atactggatc accggacgaa
    24541 tcgtttgata ggatataatt tggtctttta cttgcttttc ttcttcagga gacagctttg
    24601 tcaaatccag ttcataacca aaaacagcgc tcatagctgt ttctcctctt gttttcaacg
    24661 aagtagatct gtcggtctga tgatttggta cagctgatac atgagctgtg atggacgaaa
    24721 tcggataagc aaggcttgtt ccatattgga ttttcatacg ctccactgca tccgtgttat
    24781 cacttgtcca tgactgcggc atataataaa taaacccggc atcaaaccgt ccacctccac
    24841 cagaacagcc ttcccataag atttccggat aatcagttgt caaatgttca agtagttcat
    24901 aaagcccgag catataacga tgcgctactt ctccttgtct ctgtgggtca agggtaatcg
    24961 aatagacatc tgaaagcgaa cggttcatat cccatttgat gtagtcaaga ggaatcgtat
    25021 ctaaaatctt tctcatttgt ttctcaatcg tctgccgaac atctaacctt gtaaaatcca
    25081 gtacatgttg tgatctggaa gcagaaggca ttctacctgg ctcctgcata agaaaatcag
    25141 gatgtgttcg atacagttca gaatcaaccg agatcatttc aggttcaaac cataaaccaa
    25201 atttcaatcc tttgctatga gcataatctg ctatttcacg taatccattt gtcaacttgc
    25261 cttcatactc gaaccagtct cctaatgaag aattgtccgc atctcttttt ccaaaccagc
    25321 catcatctaa tacgaacatt tcgatgccta gctcctttgc ttcatcaatg atcgccttga
    25381 ttttttcact attgaagtca aagtaagtgg cttcccagtt attgaccaga atcggccgtt
    25441 cagcaaattg atgacgacct cttgcgattc tttcacgtag caactcatgg tggatctgac
    25501 tcattttgtt caatccctga cttgagtaag aaaggatcat ttcaggtgtt tgaaaagact
    25561 ggcctgcatt caattcccac gaaaaacgat ggctgttgat cccaccgacg actctcaatt
    25621 gattgatctg atctttttca agttcgaaag aatggttgcc ggaataaaca aattgaagtc
    25681 cgatcgcttc tccagtattt tctgtcgtat gatgatgaac taaagcaata aagttattca
    25741 tatggtgact gctcgttcct cgacggcttt caaatacttt tgttccagat aaaacagatt
    25801 gccgtgaaat ctgtctttca ttgacatgtg ctcctggcaa agcaatgact tcatcaaatc
    25861 gcctagtatg ggcaaagtct agctggaaac tagccgcttt ttctagtttg attgtttctc
    25921 cggtttcatt tcttatcttt acagatcgtg tgatcactgc tctatcagta aatatggtat
    25981 agaacaaatc gaaataaatc gctgtttctc tatccttaag tgaaatcacc aacgtttgtg
    26041 cctctgactt gtccatcaca taagtttgag gcaagccttc gatctctggc ttcccatctg
    26101 taatataatg gctgtcatat ctcagatcca gtaagtctga tccattcttg cgctgaatga
    26161 tcgatgcggg cgttcgaaag tccatgctgc catgagaaga gtattcttgc ggtagtgtat
    26221 ctttcgacag agaccggtca ggatttaagg gaacatttcc tgaaaatcca cgatctcttc
    26281 ggggataatt tttattgtct ttgtactgct tgactggttt gccaaaataa atatgcgcca
    26341 atacattgct ttcttctatc tttatcagat aagaaagaaa tgtatttcgt aaatggaaga
    26401 ctcggctctt ttcatcaaat aagatgaaat ttttatctgt ttttctatgc gtaatatcca
    26461 tcatttcact cccaacaaaa cggaaaccgt ttactttttt ttataattcc tgtatactgt
    26521 ttactaaaca attttgtcaa taaaaatttt ttattttttt gaggagtgtg taaaaatggt
    26581 tggaattaga gatgtcgcac gaaaggcaaa tgtctctcct gctacagttt cccgtgtgtt
    26641 gaataacgat cagacgatcc atgtggctca gactactcgg caacggatag tagaggcagc
    26701 tgctgcgttg gattatgaca tcagtactcg taaatatatc aaaaagagaa tgccttcgat
    26761 tggtgtgatc tctacgatcg gcaaagcgag cgaagaacag gatatctatt acaaacattt
    26821 gcgagtaggg atggaagaag aagctcgacg tctgcattta gggatgaatc gaatctataa
    26881 tctatctgat aatcctaaac aatgggaaga tctagatcag ttaggtgcgg tgatcgttgt
    26941 tgggactgtt acgaaagaat cgattaccgc cttgttaaaa caaaacatgc aaatagtcgt
    27001 cgtggataac cctgacatcc agcaagaggt cgatatggtg tatgcagatc ttgaacggat
    27061 gactaaaacc gtactggaat tgttcttaga aaatggtcat aaaagaatag cctatatcgg
    27121 gggatataaa gtcgatatgg atgaattcgg tgggaagcag atttctataa atgaaaagag
    27181 gatgcgtgcc tatcagcatt ttatgatcga tcatcactta gaaaaatata tgtcttatca
    27241 tctaggtggc tggacagaaa aacaaggtga ggtacttgcg actaatctat tagaaaatga
    27301 atcccctctt cctactgcca ttcttgtcgg gagcgatcca atggcaattg gtgtctatcg
    27361 ggcattgaaa caaaaaaata tccaaattgg agaagagatt gttgtagcaa gctttgataa
    27421 tatcgagaca atcgcaaacc tctcccctgg cttgaccagt gttcaaatca atgcgaaatc
    27481 aattgggcaa gcagctgttc gaatggcatt ggagcgaatt gacggtatac gggacgaacc
    27541 aatcattttg acttatcctg cacgattgat tgttagggaa agttttatcc caagaacaaa
    27601 aaggtattga aagagaggtt gtgaaaagcc gcttcgtttc ttcatactca aaacgaaacg
    27661 gctttttcac aacctcttac ttatacttat tcacaaaatc caatgtctgc tggtaacaat
    27721 tttgcgattc ttttagcgtg taatcaaatt ggtattcatg ggtaatctct ttttttgtat
    27781 ctgtataaaa caaggattgc acgggtacct ttaattctgt caaccgattt tctaatgctt
    27841 gaccttgctc ctggaatgag tatgcatttc cgtcagttat atacgttggc ggaaattcct
    27901 tagaaacatg gtcagccaca ctagcttctt tcaattgctc actcgttttc caatcttttg
    27961 ttccaatcaa cgaccacgca acggttttga cgaaaaactt cataaatcga tcatccgaat
    28021 gctgctgtgc tgttagcttc aaatcaacag gtccgcaata agaaatggtt cctttcagac
    28081 tatcttttgg aagtagatgt ttgattttca tttgtttagc atacgcatca ttcgtttgta
    28141 tagctgcata ttgcaatgcg atttgtgaac ctgcactatc tccaccaaaa aataattgtt
    28201 ccatgtctag caatttgtcg tctttttctt cagctaataa atctttgatc agttcattca
    28261 cttgaagaac ttgattaggg tattctgaat caggcgctaa ttcataattc atcgcaacca
    28321 ctgcgatgtt ggcgtctgaa actaattttg tcgcaaattc tttgacaccc gatttatctc
    28381 cgccgacgaa tcctccacca tgtacccaaa atagaacagg aacaggcccc ttgctgtttt
    28441 ttggatagta gatgtcataa gtattctctt tatacttgga ctcatacgtc aaatctgttt
    28501 ttttcgccac gtttttttct gcttgatcga acgttttttt atcagtgatt tgaacagtat
    28561 ttgaaaacat atggttgatc aagaatgctc caggtctggg ggaaagctgg aaggcaagaa
    28621 agattgcaaa cgcgatccca agaatgattc cgaagaacca tccgataaat ttaagaattt
    28681 tctttttcat aaggctccct cagtagttag tattaagcat tctgattata ccatagtgtt
    28741 atttcctatc aagctctcct agatgattta atgattttcc ccaaacttct tctgcaatgc
    28801 ttttgacaaa agaaattttt tccattgttc ttcaaacaaa acattttctt cttctgttga
    28861 agcaaatcca cattgaggac ttaagcacaa acgatccaaa ggcagatatc gtgtcgcttc
    28921 gtagatccgg tcaatcactt gctggtgctc ttccatttcg ccatttttcg ttacagtcaa
    28981 aatctaagac aactaattta tcttcagaga ctttctctaa aagaacaaaa ctcccagcac
    29041 gttcactatc gtattctaaa taataggcac ggacattttc ttgagtaaaa taatgggtct
    29101 gctacgtcgt catcccccct gatgccgcca aaatcgaata ataatttccg cgtcaaacat
    29161 gtatatttag tgttgaatca tttgagaata tgaaaataaa aatctttttg atcaatcagt
    29221 taatcaaatt gaaataacga aaaatctgag acagaatgac gttcattctg acccagattt
    29281 gattttgcac aactcaacag tccttattgg caaatcgcca aaataatgta gactaactaa
    29341 aaagactaag aaacaataac aaaagttaac acttcttagt ctttctttag cacgtctatt
    29401 tattaaagat tttagggcct tttctcatgt ttttacgatc attcttaggt gtttccattt
    29461 gtttggatcg gcctccacct tgtttctttt tgatcaattc tttcattttt tctttttggt
    29521 ccatttttat tctcctttaa cgaatgaagc gactaattcg atatgatggg tttgcgggaa
    29581 aagatctaca ggctgaactt tcttcacggc ataacctgct tctgcaaata acttagcatc
    29641 tcttgcaaaa gttgccggat tacatgagat atagacgatt cgttctggtg acatttcgat
    29701 tgctgattca ataaaatgat gatctaatcc ttttcgtgga gggtcaacta cgatcacaga
    29761 tggtttgatc cctttttctt gccatttttt cataactttt tctgcttttc cgacttcata
    29821 atgaacattt tcgattttat tatttaaagc attgaactgt gcatcttcaa ttgcttctgg
    29881 aataacttcc atcccataca ccttatccac tctgtctgct aaagacaagc caatcgtacc
    29941 tattcctgaa taagcgtcga tcacaatgtc ttttttatct aactcggcaa actcgatggc
    30001 tgtttggtac agatactctg cttgttgagt atttacttga taaaatgact ttgctgagat
    30061 tcgataggtt tttcctaata gctgatcttc gatatagtct ctgccatata aaactttttc
    30121 tttgtctcct aaaatcacat ttgttttttc ttcattgata ttttggatga ccgaaaccac
    30181 ttctggcaat tcttcatgaa tagcttcagc aatttccttg gatttaaaga atttttcttt
    30241 acgagtaact aaaacgacca tcatttcatg ggaataatga ccacgacgga taatgatgtg
    30301 acgaagctgt ccttcatttt tttcttcatt atatcctcgt acttggaacc gttgcaagat
    30361 atctctgact actttgattg cttgatcgat gacaggatct tggatataaa aatcttcgat
    30421 agggactaga tcatggctat tctttttgta aaaaccggtg gctaactgtc catcgatacg
    30481 gcgaacaggg atctgtgctt tattacggta gccgaatggt tctgccattc cgaccgttgg
    30541 aagtacagga acttctggca tcttagcaat cttcgccatt acatgctcta cttgtttttg
    30601 tttgaagaca agctgttgtt catacttcaa atgggctaaa ggtgcgatac ctatgcgaag
    30661 taatgcattg ttttctacag gctgacgatc aggtgattgt gtaataaatg tttcgacttt
    30721 tccatatcca aacttattgc ctgcttttac tactagtatt tctacttctt ctccagggag
    30781 tgcattttcg ataaataatg gataaccatc gatttttgct acccccattc cttcataact
    30841 caagtcgacg atttcgactg tatatcgttc gtttttcttt actatttgct gttctgtcat
    30901 attttgctcc tttaagccta tttttcaatc ctactgaact ctactataaa tcagctaatt
    30961 acacaactaa aaccggcact gaccttacta cgttcagcac cggcttttga ttaattcttt
    31021 tgattaggtg tttcgtctgc tatttttccg tcaccgtcga tatcttcatc cgttaaacgt
    31081 tcgacttctt ttacgaattc tttgctgaca acctctaatt cttcttcatc tgtacctgtg
    31141 attgcttcat caggaatctg atcggtattg gcaaagaact caatgtgttg atgaagattt
    31201 tggaaatgca tcggtgcttc cccgccgtat tcaccatcta agttgatcat tactggtcga
    31261 ttgatatcat cttctgtttc aacgaacaaa ctgcttgttt tggtatagat tattcttgga
    31321 tcatcgacat gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat ctcaaagata
    31381 tttgctgtct tcacaatgat taaagaaaac ttcccatcat ccaatttggc atctggcgcg
    31441 atctgttcga atcctccaac agagttcgtc agccctagga aaaacatcga agcatttccg
    31501 atatattccc cttcatcata cttcaagcgc attttgattg gctttactcg agggagcatc
    31561 tctgctcctt tcgcaagata agcaagataa ccaaaaatac tctttagttc tgatggaact
    31621 tcataagtca attctgtcaa atagccacct gcagcgatat tgatgaagta actgttatca
    31681 gaaaggccga tgtccatttt tacggtttgg tgttttttta tcacttctgc tgctgcttta
    31741 atattatctc taggaatttt taatgctcga gcatagtcgt tcgttgtacc agctgggatg
    31801 atggccattt taggacggtg ttcaagcgga gcgattccat taaccacttc gttgattgtg
    31861 ccatcgccac cagctgccac tactagatca aaacctagtt ctgcgactct acatgcttca
    31921 ttcttggctg aatctggttc tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct
    31981 tctaatgcct gtaggatatc cgccaaattt tttttcaata attcttttcc cgaggtgggg
    32041 ttataaatta cgcgtgcttt tttcattttc agcctctatc gtttcgctaa ttcatcttta
    32101 agtagcttat tcacgacacc aggatttgct tgtcctttag ttgctttcat gatttgaccg
    32161 actaggaagc caaccgcacg atcttttcca tttttgaaat catcgacaga ttgttggttg
    32221 ttatccaaaa tttcattaat gattggcagt agtttgttcg gatcagataa ttgcacccaa
    32281 ccattttttt caactacttc ttgggcttct ccgccatttg tgatcaattc acggaagact
    32341 tttttggcga tttttgagct gatcgttcca tcggcgatca aacgaatcat tcctgctaag
    32401 ttctcaggtg tcaattttgt ctcagctaat tccagtttct cactgttcaa gtaagcagaa
    32461 acttcaccca tcaaccagtt agatgcttgc ttggcatctg caccattttc caaaacagct
    32521 tcaaaaaaat cagacatttc ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt
    32581 ccaaactcac ggatatagcg ttcgcgacga gaagctggca tctcaggcaa actagctttt
    32641 actttttgaa tccattcgtc atcgatcacg aatttcggga tatcaggctc tgggaagtaa
    32701 cgataatcac tagacccttc tttgacacgc atcaaaactg ttttattcgt tgtttcgtca
    32761 aaacggcgtg tttcttgttg gatttctcct cctgaaagaa gaacttttgc ttgacgtttt
    32821 tcttcaaaag ctaaaccttt tttcacgaaa gataaagagt tcaagttttt caattctgct
    32881 ttcgtaccga actcttcttg tccataaggg cgaagagaaa tattggcatc acagcgcata
    32941 gagccttctt ccattttcac atcgctgact tctgtaaatt ggataattga tcgcaatgct
    33001 tctagataag cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac gatttcgatc
    33061 aaaggcgtgc cttgtcggtt caaatcaacg taagaataac cgccaatacc atgcatgttt
    33121 tttccagcat cttcttctaa atggacacgt tcgatccgga tttttttctt tttaccttct
    33181 acttcgattt cgatccatcc gtcatgaccg attggttgat caaattggga aatttgatat
    33241 gcttttggat tatctgggta aaagtaattt ttccggtcaa aatgagtatc tttagagatt
    33301 tcacagttta atgccaaagc tgctttcatc ccgaattcca gcgcagcttt attcattact
    33361 ggcagcacgc ctggataacc ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca
    33421 aaatgagctg gcgctggaga gaaaattttt gagttggttt ttaactcgac gtggacttct
    33481 agtccaatga ctgtttcaaa attcattcgt ctttccctcc taaaatagct ggttgttgtt
    33541 tatggaattc tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa
    33601 aataattacc gatgatctgc aatccaactg gtaatccttc tgataaacct gccggtacag
    33661 acatacctgg aagtccagcc aagttgacag ggatcgttaa gatgtcactc atgtacattg
    33721 tgattggatc gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa gatggcccga
    33781 tgatcaagtc ataatctgca aagacttttt caaaatctcg tttgatcaat gtacggactt
    33841 gtcctgcttt tttgaagtaa gcgtcataat aacccgcgct taaggagaat gtacctaaca
    33901 tgatacggcg ttttacttct tcaccgaatc cttctgaacg tgagttgaca tatacatcat
    33961 ctaatgattt cacattttca gagcgataac cgtagcggat accatcgaat cgttgcaaat
    34021 tggaacttgc ttctgatgag gcaatgatat aataaacagc cacaccgtat tttgaatgag
    34081 gcaagctgac ttcttccaca acagcaccta atgaacggaa tgtttctgct gctttcaata
    34141 cagcttcttt tacgccaggg gccacacctt cgcctaagta ttctttaggt aaagcaatct
    34201 tcattccttt gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag
    34261 aagtaccgtc tttttcgtca taaccgctga tagctgtcaa tgctaatgca ttatctttca
    34321 ccgtacgggt cattggtccg atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac
    34381 gtgagacacg gccataagta ggtttcatcc ctacgatgcc gttaaaagct gctggttggc
    34441 ggatacttcc cccagtatca ctacctaaag aaactggaat ttgtccagct gcaactgctg
    34501 ctgcagaacc accagaagag ccaccaggta cttttgttgg gtcccaagca ttttttgttt
    34561 tcttgaaata agaagtttct gtacttccac ccatagcaaa ttcatccatg tttagcttac
    34621 caacagggat catatctgct tgatagattt tatccatgac cgttgcatca taaattggtt
    34681 caaaattgta aaggatctta gatgcagcgg ttgtcaagat atcttttgtt acgatattat
    34741 ccttgatacc aattggaata ccagcaagga cgttgctttc atcaatccct ttttcatcta
    34801 aagcttttgc ctgttccaat gctttttctt cgttcaatgt aatgaatgcc tcgacctcag
    34861 gctctgtttc tttgattcgt tcaaatgttg tttttgtcaa atcttgtaca gtgatttctt
    34921 tcgatacaag catatgatgc agttcttcta gagaacgatt gtgtaattct gtcattatgc
    34981 gccagcctcc ccattatcga tgattgcggg tactttgata aagccgtctt ctgtttccgg
    35041 cacatttttc aataattcat cgcggttcca tccagcttgc gcccgatctt gtcgcatcac
    35101 gtttactgtt tcaaccacat tcgacgtaaa tggaacacct tccgtatcta cttcttccag
    35161 catttcaacc atatcgatga ttttacctaa ttggtcggta aaacttgcaa gttcttcatc
    35221 tgcgaacgca agttttgcta atttggcaac gtgtttcact tgttcttcac taatcgccat
    35281 actacccctt ctttctaaac gagttaaaaa ctttgctctc tattttaaca caagagtgcc
    35341 gtgtctgaaa ttgtttattg aaatttgtct gaaaaaactc atttttcatt cttctactga
    35401 gcgactttta gccaatgcat ctgccatttg atcttctgtc caaacttcga ttcctaattc
    35461 ttgtgctttg gtcagcttgc tgccagcatc ttctcctgct accacgatat ccgttttttt
    35521 cgatacgcta ccagtcactt tcccgccaag attttcgatc gtctcttttg cttcttcacg
    35581 agtaaatcgt gttagtttac cagtaaggac tactgttttt tctttaaaag gagactcgac
    35641 ttcttgcaat tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa
    35701 ttcattcatc aattcatgga cttcttcgtt actgaaataa gtgactacac tgtcggcaat
    35761 gatatcaccg atcgtatcaa gcgcaataat cgactcatag tcacttttac tcaatgtttc
    35821 tagatcaccg aaatgttctg ctaaaatttt tgctgctttc gctccgacat gtcggattcc
    35881 taacccgaaa atcaaccgct cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat
    35941 attatttgct gatttttctt ttattttgtc caacgtcaat aactcttctt ctgtcaattt
    36001 gtaaagatca gcaacatctg ctactagttt tttatcatac atctgttcta gtacgcgcgg
    36061 ccccaaccca tcgatattca tcgcatttct tgaaacaaag tggttcaaac cttctttcat
    36121 ttgagcaggg catttaggat tgatgcaccg taaggctact tcttcatcca aatggacaag
    36181 ttcacttcca caaactggac aatgtgtagg caattggtat tcttcgctgt ctttaggtcg
    36241 tttatctaac acgacttgcg aaacttctgg gatgatgtct cccgctttat aaatcagaac
    36301 tgtatctttc aagcggatat ccttcaactt aatataatca ccgttatgca ggctggcacg
    36361 gcttactgtc gttcctgcta ctcggactgg tgtcatgata gctgttggcg ttactacccc
    36421 tgttcgaccg actgtccatt cgatgttttc gattagcgtt tcaacttctt ctggcggaaa
    36481 cttgtaagct gctgcccatc tcggtgcttt gatagtaaaa ccaagttgat cttgtagcga
    36541 gaattcattc actttgatga cgattccatc gatttcatag ggcaaatcca cacgtttgtc
    36601 atgatattct tcaatgtatg accacacttc atcaatcgaa tgacatagac gtttttcccg
    36661 attcgtgtgg aatccaattt tttctagctc ttctagcgca tcatactggg tttttgcttg
    36721 cataggaccg aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat
    36781 tttagaatct aactgacgca aagaaccagc tgcggcgttc cgaggattcg caaagatatc
    36841 ttttccctct gcttctctgt cttggttcaa ttggacaaat gaccgttttg gcatgaaaca
    36901 ttcgcctctt acttcgatgt tcattggttc ttttaatttg attgggaccg accggacagt
    36961 ttttaggttt tcagtgatgt tttctcctac agttccatcc ccgcgagtcg cacctctcac
    37021 gaaatttcca tcttcataac gcaaagaaac ggaaagtcca tctattttta gttcacagca
    37081 atagtcaact acacgtccca ctgctttttg tacacgttga tcaaaagcta tcagctcttc
    37141 tttgctgaaa acgtcgttca aactgtaaag cggaatatca tgagtgactt tttcaaaacc
    37201 ttctaatact tttccgccta ctcgttgagt gggagaatca gacgtgatca aatcaggata
    37261 ttgttcctcg attgctacta attctgcgta ttctttatca taaacatagt cttccactgt
    37321 tggtttatct tctacgtagt attcgcgaga ccattggttc agtcttgtac gaagttcttc
    37381 tgctcgttta gcagcttcgt caatcgtcat ttcatgttcc atcatttacc ctccactact
    37441 aagctttttc aattggcgca aaggcagcca aaagacgttt tactccttga cttgggaaag
    37501 ccacatctag ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt cctagacccc
    37561 attttttgtg tttgacttta tctccaggtt tccagctgtt attttcccct ccagtagcac
    37621 ttttagaggg aacaaccgct gtctctggct gtttatagct atatgctgtt tttttagcga
    37681 atacaggcgt ttttttaggc gtaggacgca tgccttcgat ttccagtaac tcctgatcga
    37741 tttcttctac aaaacgagaa ggtcggttat attgcgtccg tccatataat gttctggaga
    37801 aagcatttgt taaatacaac gcttcttctg ctcgcgtgat ccccacatat gcgagacggc
    37861 gttcttcctc cagttcgctt tcttccatca atgctcgaga taaaggaaaa accccctctt
    37921 ctagaccaat caggaataca actggaaatt ctaatccttt ggctgcatgc aaagtcatca
    37981 atgtcacttg ggaagcatct tcttctaaat tgtcaatgtc ggaaacaagt gccagatcat
    38041 ttaagaagac cgttagtttt tcttctggtg catcagcgtc ttcttcattt tgctgttcga
    38101 attgtttgtc gaattcttgt gtgaccgtta aaaattcctc caagttttcg atgcgtgctt
    38161 gtgcttctaa tgtattttgg atttttaagt cttctagata tccgctacga tccaaaacct
    38221 ctttcgtcaa ttctgtcact gtcaagtatg ggatcatctg agtgacttct tggatcatct
    38281 ctccaaatgc acctaattgc tgtcctgctt ttccaccgat atttgccaga tccacatttt
    38341 gcgctgcttc aagcagtggc cattcatgaa gagaagcaaa actgcgtagt ttttcgatag
    38401 atcctggtcc gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg attgaatctt
    38461 gaggatttac cagcacattc aaatacgcta aaatatcttt gatttcttta cgatcgtaga
    38521 acttgtgccc tccaaccatt ttataaggaa tatttgcttt caacagagtt tcttccatta
    38581 cacgagattg tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc cgatgattgc
    38641 ttcgaatctc ttcttgcatc cggtctacga taaaacgggt ttcgtctcgt tcattatctg
    38701 cacggtagta agtgatcttg tttccttctt tgttttctgt ccacaagttt tttggttttc
    38761 gattgctatt gttttcaatg acttggtttg ctgcgctaag gatgtttttc gtggagcgat
    38821 agttttgttc tagcaaaatt actgcagcat ctggataatc tttttcaaaa tccaagatgt
    38881 tctgcatatc tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc acgacgcaca
    38941 ggttcctaaa tctacctgcc aataaattga ctagcgtata ctgtgcgtga tttgtatctt
    39001 ggtactcatc tacatgaata taatggaatt tgttttgata atacgtgagt gaatcaggat
    39061 gttcttcaaa taatcgaatc gtattcatga tcaggtcgtc aaagtccata cattgattat
    39121 attgaagctc tttttggtaa gcagcataac attttgccgc aatctcttcg taataactgc
    39181 cttgcatttt tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga gagattgttc
    39241 ctaatatact tcgaggatca tattttttcg gatcaatgtt cagttctttt agtatccgtt
    39301 tcatcaaggt cagctgttca gaagaatcga tgattgtaaa gttccggtta tacccgataa
    39361 aatcaacatc tcttcttaaa atacgcacac acatcgagtg aaaagtcgat acccagacat
    39421 cttcgcctcc agatgcgaga atcgcattga cacgttcttt catttctttt gcagccttat
    39481 tggtaaaagt aatcgctagg atgttccaag gattgacttc tttttcctca atcaaatagg
    39541 caatgcggtg tgtcagcact ctcgtctttc cacttcctgc acctgccatt agcagtaatg
    39601 gtccgtcagt gtgaagaaca gcttctttct gacgggggtt catcccgttt aataattcag
    39661 ctttggatga cattaacaac atcctttctt ttatcaatct tgttaattat agcattttct
    39721 tggctttcat taaactagct agttcaaaat catttgtttc tttttatgga tttttccttg
    39781 tcaattcgtt gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag
    39841 gaaaaatcaa gaaattagat aagactcaga tatattttat tctaaaagga atatttgttg
    39901 gactgctgac aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt
    39961 tagttcgaag tatctatgaa atgagtcggg aacagccgat ttacttaatt ggcctagcta
    40021 tttgctgcat ccttgcagca tttttcgtgg gttatctggt caaaaatgaa ccggatatca
    40081 agggaagcgg gattccacaa gtagtgggac agcttcgcgg ggaattatca atgaactggt
    40141 tctctgtttt atggaaaaag ttcattggcg gtgtactttc agttggtgcg ggcctttttc
    40201 taggtagaga aggaccttct atacagcttg gcgcatccat tggacaagga gctggacaat
    40261 tgtttcggag tccatcttca gaagaaaaga ttttaatttc tagcggtgca agtagcggac
    40321 ttgcagcagc attcaatgct cctatcgcag gattactctt tgttttagaa gaaatccatc
    40381 actccttttc tccacttgtt tggctgacat catttgcttc agcaatcact gctaacttcg
    40441 tctctcttta ctttttcggc ttgagacctg ttttatatct aggtgacctt ccttctttgc
    40501 cgttgaaata ttatggttcc ttagtgatct taggagtact tctaggcatc ttaggtttta
    40561 tttatcagaa tgtcttgctg gctttgccaa aattttacaa ccgattgcct ttaccagctt
    40621 attttcacgg gttcgttcct ttcttgctga ttttgccaat tggttattta tggcctagta
    40681 ctctaggcgg aggcaatcaa atcgttttag cttttggaca tgcttccttg ccaatcatta
    40741 tgattatcgg tttgtttgtt cttcgattcg ttttttccat gatttcttac ggtgggggtc
    40801 ttccaggggg gattttcctt ccgatattga cattgggggc tttaatcggt agcttttatg
    40861 gaaacattat ggttgatttc gggatggatc caatctacat caaggatttt gtcgttattg
    40921 ccatggctgg ttatttcaca gcgattggaa aagcaccgct gacagcaatt attttagtga
    40981 cagaaatggt tggctctctt aatcatctga tgcctttagg attggctgct cttgttgctt
    41041 atatcgtcaa tgaccttctc ggcggtaatc caatttacga atcattattg gaacgtctac
    41101 ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac aacttttgat tttccagtaa
    41161 cggcagagag ttctttagac ggattaatgg tacgagactt caattggcct aaaaatatgc
    41221 tgctaatctc tatacgcaga ggaaatcagg aaatcttgac tcacggtgat acgatcatga
    41281 atgtaggaga tattttggtt atcttgactg atgaagcaaa cttgccaaga atcaagcagg
    41341 aaatcgaggc taaatcttct atcacaaatt tgaagcaatc attttaacga acatggaaaa
    41401 actattttac aacaactatt catctacact acttgattct atatgattct ataaaaaaag
    41461 aagaggctgg gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag
    41521 cttttctcaa taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca
    41581 aatacatgac cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta
    41641 atttgattcc gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg
    41701 gaatggcacg aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata
    41761 gtcccatcac gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa
    41821 acaaacgtgt agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag
    41881 ccatcacgac acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat
    41941 aagcggcttt gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac
    42001 caagtaaagc agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga
    42061 ttgctttcat agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca
    42121 ctggataaaa gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt
    42181 tacgtaaaaa tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc
    42241 ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt
    42301 caccgatact tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt
    42361 gtccgatttg gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat
    42421 catgtggcac gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac
    42481 caataacgaa aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc
    42541 cgatagtacc atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata
    42601 ctggtgcagt accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac
    42661 tgacaggacg attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg
    42721 ctgcaacgat tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc
    42781 cttctgaacc gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca
    42841 aagcatcttc tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa
    42901 cgaattttct ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt
    42961 cttctgcttc tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat
    43021 ttgcttcttt taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta
    43081 atgcttgcaa atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag
    43141 ttggctgtcc gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg
    43201 ttgcttcttt aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg
    43261 tagaagtttg tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc
    43321 ggccggcatc aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt
    43381 gcaaatccat gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc
    43441 ttgaaactaa tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg
    43501 agctgccaag tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc
    43561 ttcgattgga tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc
    43621 tgcacctact gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc
    43681 cgtgaagagc aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc
    43741 tagtaatttt ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag
    43801 ctcagctaat tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt
    43861 taggtcatca cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat
    43921 cagttcttga taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat
    43981 atctccttgt cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat
    44041 agatgggcct aagccattga tctcagtttc agtttctgat tttagtttga tattgattct
    44101 tgtatcttct tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga
    44161 aataaattcg ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg
    44221 tttcaaaata cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat
    44281 tcggttcaaa tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt
    44341 aagtgttact gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca
    44401 ttaacgaatt ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca
    44461 gaggtgaagg acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa
    44521 tttttcatgg ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt
    44581 tcaagatggg caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt
    44641 tctatcgcat tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata
    44701 atggtagcaa gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact
    44761 gtgatctgtt ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa
    44821 taaatgacat cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg
    44881 tgaacgtttt tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc
    44941 ttagctccgc catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt
    45001 cttatcgttg attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc
    45061 tgctgttcaa gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc
    45121 acctcaatac aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg
    45181 ttcataaaaa ttataaatca gtcaaaaaaa aataaaaaac cagtcagaaa gactggtttt
    45241 ttattttttt gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt
    45301 tcatataaac agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc
    45361 cgccaaacat gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga
    45421 gacagacgga taataatgat gccggaacca ttattcgata atagagttgt ttatccattt
    45481 tcttcacctc atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat
    45541 tgcacacgca gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc
    45601 tttattcgtt tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct
    45661 gacagggatt ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca
    45721 tctgttacct ctacttctag atattcccta atataataac aataagcttc gattccatta
    45781 tccaaagcaa cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca
    45841 acccaaggaa aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct
    45901 ccttgttgga tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt
    45961 tgcatttgtg cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca
    46021 acgaatggcg gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc
    46081 tggtccctaa aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct
    46141 cgataaagct ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg
    46201 atgtcgagaa aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc
    46261 caacacgaag tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa
    46321 tcgactacta cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt
    46381 ttctcaaaca atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa
    46441 gctcgcatat aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc
    46501 aaaaattgct tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta
    46561 tcttccaagc gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct
    46621 cttaccgatg ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt
    46681 tgtgtgaaag agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg
    46741 cttatttcct gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat
    46801 ttatcttcgc caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca
    46861 atgataaaga aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag
    46921 actaagtaaa aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata
    46981 atttagataa acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag
    47041 aagagatcga tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac
    47101 aaaagtaagt taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact
    47161 ggtaagtctt ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc
    47221 ttcttttccc tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc
    47281 caacgctttt ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc
    47341 cttagtggca acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat
    47401 atcttcttga gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc
    47461 tctagtcgtt aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac
    47521 ttttgcatac ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa
    47581 attcaatgtc tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat
    47641 caatgtgttg ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg
    47701 caattgtgta tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg
    47761 gactttcatg gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag
    47821 ccaaaatgac ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat
    47881 agattactgt tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga
    47941 tgttttcatt tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac
    48001 gaagtcctcg agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt
    48061 caggttcgaa ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta
    48121 gcgcattttt cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag
    48181 ctgccataac aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt
    48241 caggaatgat atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa
    48301 atccaatcgt cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac
    48361 cacctacgat gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt
    48421 tacgtccgcc ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt
    48481 gttggacacc ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa
    48541 ttttatcgat ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag
    48601 cagattgaag caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg
    48661 tcaaacttgt cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg
    48721 ctaaaaatgt tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt
    48781 caacatcatc tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg
    48841 caacagctaa cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa
    48901 gaatttctag tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt
    48961 catcaatgat ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag
    49021 ctacgatctt tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat
    49081 tgctacttgg attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa
    49141 ctatcaaaga ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt
    49201 ttaacaaagg atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga
    49261 actgtgacaa aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct
    49321 tcttcagaaa tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga
    49381 tcaattcgat tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc
    49441 gtttgatttg ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga
    49501 tttcttcttc tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg
    49561 ttcttgtgcg catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac
    49621 cagttaattg atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt
    49681 cgtccattga acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg
    49741 cgttttcaac agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt
    49801 ttgtttctac taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt
    49861 catcaacatc ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat
    49921 ggattgtcac tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg
    49981 ggaaagttac atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa
    50041 aacctggaat gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat
    50101 caaacgcttc gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag
    50161 ctgcagcatc ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg
    50221 catccacgtc agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt
    50281 gtcctaattt tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt
    50341 tttccatgct ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga
    50401 cagcagcttc ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt
    50461 caccgtacat acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt
    50521 tcaagctttt tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg
    50581 caatcgtaaa ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt
    50641 aattccctcc gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta
    50701 aatagtacac taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc
    50761 ataacatttt tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa
    50821 ttttttctct tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt
    50881 ttcctgtaca cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat
    50941 catagacagg ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa
    51001 gcagcgcatc ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct
    51061 gctgctgcgg ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca
    51121 aatagaaaac aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg
    51181 ctaaaatatg gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat
    51241 aaggaagctg ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa
    51301 gttcctccga tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt
    51361 cttctgcaaa ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac
    51421 ttgctatcaa ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa
    51481 attcagcaaa ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg
    51541 cttctttttt ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc
    51601 tttctttcaa ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg
    51661 ctagtgcttg ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct
    51721 tcacgcagtg tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa
    51781 aagcatgaca attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt
    51841 ttagaaggtg ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt
    51901 tgaacgcttt gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat
    51961 gttgcttttt ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta
    52021 gaatgaaccg gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat
    52081 caaagctgat gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt
    52141 tcgtcaataa aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca
    52201 gataagaatc tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg
    52261 taatagctcc gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg
    52321 catagaaata cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact
    52381 ttttccattt acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag
    52441 agctcagtac cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat
    52501 ctttttcttg ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata
    52561 atacattgct tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact
    52621 agtctcagaa ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc
    52681 aaacccagat ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt
    52741 tcgtatagct tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt
    52801 tgcttggaca ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt
    52861 attccgtccc tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg
    52921 aagcaccgct tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc
    52981 tccgctttta actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa
    53041 tcctgacatg atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc
    53101 tgcttgagca gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat
    53161 tgggatatca tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta
    53221 gacaccgcca ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc
    53281 aatcgccttc agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc
    53341 tgaagaccct cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact
    53401 gtctccctct tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa
    53461 tccctcaatt tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac
    53521 aaataatcct gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt
    53581 ctaatacaag ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc
    53641 tgctttttct tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt
    53701 aaactcacga gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca
    53761 gccagtatct aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga
    53821 caattgtttt tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc
    53881 tcctctatcc gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc
    53941 cattattttt tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt
    54001 tttattcttt ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag
    54061 ataaaaaaca aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca
    54121 gatgacgagg agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac
    54181 agaaaatcca caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag
    54241 cattcatacg aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg
    54301 attggattaa aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt
    54361 ggttatgatt cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca
    54421 caaggcagaa ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt
    54481 attggtcata cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat
    54541 acttcccaaa gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa
    54601 cttaaaattg cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc
    54661 gctcatctaa ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct
    54721 ttgcgggtca tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt
    54781 atttacgtcg ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg
    54841 attgccagcg atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat
    54901 gaagagatgg ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc
    54961 gtagaacgtt cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat
    55021 cctttttata tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa
    55081 aaatacgtaa cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct
    55141 gatagcgatc gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt
    55201 aaacagactt tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt
    55261 ggctataaca cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc
    55321 gaaacagcag acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt
    55381 acgattacaa atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg
    55441 catgctggac ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta
    55501 ctaactttat tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc
    55561 gatatcactc atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa
    55621 gattatttag aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt
    55681 ggacgtggtg ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt
    55741 tatatccagg cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc
    55801 gaagaaggaa caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga
    55861 ggaaacctga aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga
    55921 ttagaaaagg aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta
    55981 accacagtcg taccgactca attacttgca tattacgcaa cgctattacg gggattcgac
    56041 gttgacaaac cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac
    56101 cagtgtcaga catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa
    56161 tccagtccga tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc
    56221 tcatcagtag gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact
    56281 aggattcttc cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc
    56341 ggaacatcca tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag
    56401 attgttacct ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt
    56461 aactgaattc ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc
    56521 ccagactgct cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga
    56581 tcaccaactt gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct
    56641 aagttactca tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag
    56701 tatttagcac agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc
    56761 gcaataatac gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg
    56821 accatctcag ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg
    56881 tctggatttg tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca
    56941 cttgttgaag ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg
    57001 ttgatcgttt gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca
    57061 gttcccaatc cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct
    57121 tcaatttcta gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt
    57181 ccattatctt gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc
    57241 aaacgtgtca gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata
    57301 cccaccgata aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta
    57361 tcccgcccta ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa
    57421 cctccgcacc gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg
    57481 cgtacgccgt ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat
    57541 tttctgagga atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg
    57601 tacttgtggt cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg
    57661 gttccgttcc tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc
    57721 gactgctcgt cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac
    57781 taactgattc gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg
    57841 ttcgatgatt gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa
    57901 gcaaatcgct agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt
    57961 ctaccccggc agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct
    58021 tgatcgaatc agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc
    58081 ctgctgcatt cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg
    58141 aagcgtccac atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga
    58201 tagagacttt tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg
    58261 ttgaaggaat caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg
    58321 taatagtcga tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg
    58381 gaacttcatg tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg
    58441 tatcttcgtt tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca
    58501 ctgcattttc atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca
    58561 cccccgtaac tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc
    58621 catttgcatt gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc
    58681 ttgtccgttt ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa
    58741 agattgaatc acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa
    58801 gattgccaaa tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct
    58861 gacgtccccg gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc
    58921 gacagctgcc cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg
    58981 aagataggcg cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag
    59041 cggtgtatta ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc
    59101 aatccctgtc tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc
    59161 gatttttcgc ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc
    59221 ttcctgctgt tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc
    59281 tctccggatt tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg
    59341 gttcatcagc caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat
    59401 aaaaatagct acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag
    59461 tttgtataca agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga
    59521 gaacaagtca ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg
    59581 aaatgacata aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt
    59641 taattttaga ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa
    59701 attgcaagta tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat
    59761 tcattatatt ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta
    59821 cgtcgttctg ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc
    59881 gttttttctt ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc
    59941 ttgatttttt ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg
    60001 tggacagtct tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa
    60061 aagcgaactt gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc
    60121 aaagtcattg ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa
    60181 taaccagaag aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag
    60241 ccatgtgtga tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct
    60301 tcttcaaatg ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa
    60361 acatttccat atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta
    60421 gccgaaaatt tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg
    60481 gaatagactt cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga
    60541 tcgatcccgc caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg
    60601 ttttttacga atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt
    60661 tcgctttcga tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac
    60721 atgtgtacta accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact
    60781 tctttgattg aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa
    60841 ccaaattcag cattatcttc caataaagaa tttgaccatg caggcccttg accacattca
    60901 ttggtcgtat aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca
    60961 ttcgcgatca tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg
    61021 ccacagcctg agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag
    61081 acagtattct ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa
    61141 ttcacagctt gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg
    61201 ggacaagcct ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg
    61261 tattttacgc cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc
    61321 atttcctctt catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat
    61381 tcattacaca tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc
    61441 ttttccatag aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt
    61501 gtaccaacag agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata
    61561 taaccagtgt tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg
    61621 atcgcgactt gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg
    61681 atagccattg atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc
    61741 tcatcaaaag gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc
    61801 cggcgtccca aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg
    61861 ttattttcag ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg
    61921 ctccaaagag tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag
    61981 ctatgcaggt aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct
    62041 gaacgtatcg gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc
    62101 gaatcataat aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata
    62161 gctgatttat ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat
    62221 gtttgcggtt ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca
    62281 tccacgatcc ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca
    62341 aaaactgctg agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca
    62401 ataacagccg ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt
    62461 tctccacctg cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga
    62521 ggcaactttt gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa
    62581 cctgttttcc gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact
    62641 gatcccatag aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca
    62701 taagaagttc ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat
    62761 ttatcataat aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc
    62821 gtaccactaa cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc
    62881 tcctgattga tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg
    62941 tgactcgtac ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca
    63001 agatgggcaa caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca
    63061 aatcctgttt gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt
    63121 gtcgtcactg cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg
    63181 tacatattcg gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca
    63241 ccagttttca atgatccatg gaccacacca gctgctcctg cttcggattg catttctact
    63301 acttttactg gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat
    63361 tcagccatgg tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa
    63421 gaaatatatg cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat
    63481 ccttgttcat attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt
    63541 cttttattta taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac
    63601 aaacagtcta aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt
    63661 gttttaacat ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc
    63721 tttatggttc atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt
    63781 attgccgaca atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc
    63841 aattgcagcg agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg
    63901 tgatgcaccg gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa
    63961 tattcgatac tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag
    64021 tagataaact aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg
    64081 aagctgagca agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg
    64141 atgaatctat ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataa
//
LOCUS       DACEJZ010000004        56802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEJZ010000004 DACEJZ010000000
VERSION     DACEJZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.004"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..570
                     /locus_tag="IW656_000238"
     CDS             100..570
                     /locus_tag="IW656_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0835972.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IW656_000239"
     CDS             792..1895
                     /locus_tag="IW656_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0835973.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..3075
                     /locus_tag="IW656_000240"
     CDS             2116..3075
                     /locus_tag="IW656_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0835974.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3245..3925
                     /locus_tag="IW656_000241"
     CDS             3245..3925
                     /locus_tag="IW656_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0835975.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4015..5049
                     /locus_tag="IW656_000242"
     CDS             4015..5049
                     /locus_tag="IW656_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0835976.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5063..5398
                     /locus_tag="IW656_000243"
     CDS             5063..5398
                     /locus_tag="IW656_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835977.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5382..7862
                     /locus_tag="IW656_000244"
     CDS             5382..7862
                     /locus_tag="IW656_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835978.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7918..9477
                     /locus_tag="IW656_000245"
     CDS             7918..9477
                     /locus_tag="IW656_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0835979.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9474..10838
                     /locus_tag="IW656_000246"
     CDS             9474..10838
                     /locus_tag="IW656_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0835980.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11009..12079
                     /locus_tag="IW656_000247"
     CDS             11009..12079
                     /locus_tag="IW656_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0835981.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12094..12762
                     /locus_tag="IW656_000248"
     CDS             12094..12762
                     /locus_tag="IW656_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0835982.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12782..13360
                     /locus_tag="IW656_000249"
     CDS             12782..13360
                     /locus_tag="IW656_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0835983.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13761..14672
                     /locus_tag="IW656_000250"
     CDS             13761..14672
                     /locus_tag="IW656_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0835984.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15050..15967)
                     /locus_tag="IW656_000251"
     CDS             complement(15050..15967)
                     /locus_tag="IW656_000251"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0835985.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16169..16606
                     /gene="zapA"
                     /locus_tag="IW656_000252"
     CDS             16169..16606
                     /gene="zapA"
                     /locus_tag="IW656_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0835986.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16664..17212
                     /locus_tag="IW656_000253"
     CDS             16664..17212
                     /locus_tag="IW656_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0835987.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17327..19687
                     /locus_tag="IW656_000254"
     CDS             17327..19687
                     /locus_tag="IW656_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0835988.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19774..20088
                     /gene="trxA"
                     /locus_tag="IW656_000255"
     CDS             19774..20088
                     /gene="trxA"
                     /locus_tag="IW656_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0835989.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20270..22048
                     /gene="uvrC"
                     /locus_tag="IW656_000256"
     CDS             20270..22048
                     /gene="uvrC"
                     /locus_tag="IW656_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0835990.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22193..23092
                     /locus_tag="IW656_000257"
     CDS             22193..23092
                     /locus_tag="IW656_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0835991.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23085..24347
                     /locus_tag="IW656_000258"
     CDS             23085..24347
                     /locus_tag="IW656_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0835992.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24508..26406
                     /locus_tag="IW656_000259"
     CDS             24508..26406
                     /locus_tag="IW656_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0835993.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26736..27455)
                     /locus_tag="IW656_000260"
     CDS             complement(26736..27455)
                     /locus_tag="IW656_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0835994.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27792..28124
                     /locus_tag="IW656_000261"
     CDS             27792..28124
                     /locus_tag="IW656_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0835995.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28111..30114
                     /locus_tag="IW656_000262"
     CDS             28111..30114
                     /locus_tag="IW656_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0835996.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30133..30603
                     /locus_tag="IW656_000263"
     CDS             30133..30603
                     /locus_tag="IW656_000263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0835997.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30624..31208
                     /locus_tag="IW656_000264"
     CDS             30624..31208
                     /locus_tag="IW656_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0835998.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31223..32209
                     /locus_tag="IW656_000265"
     CDS             31223..32209
                     /locus_tag="IW656_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0835999.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32199..32510
                     /locus_tag="IW656_000266"
     CDS             32199..32510
                     /locus_tag="IW656_000266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0836000.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32532..34313
                     /locus_tag="IW656_000267"
     CDS             32532..34313
                     /locus_tag="IW656_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0836001.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34306..35682
                     /locus_tag="IW656_000268"
     CDS             34306..35682
                     /locus_tag="IW656_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0836002.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35689..36324
                     /locus_tag="IW656_000269"
     CDS             35689..36324
                     /locus_tag="IW656_000269"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0836003.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36354..37709
                     /locus_tag="IW656_000270"
     CDS             36354..37709
                     /locus_tag="IW656_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0836004.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37901..39295)
                     /locus_tag="IW656_000271"
     CDS             complement(37901..39295)
                     /locus_tag="IW656_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0836005.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39387..40358
                     /locus_tag="IW656_000272"
     CDS             39387..40358
                     /locus_tag="IW656_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0836006.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40355..41137
                     /locus_tag="IW656_000273"
     CDS             40355..41137
                     /locus_tag="IW656_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0836007.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41306..41677)
                     /locus_tag="IW656_000274"
     CDS             complement(41306..41677)
                     /locus_tag="IW656_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0836008.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41811..42254
                     /locus_tag="IW656_000275"
     CDS             41811..42254
                     /locus_tag="IW656_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0836009.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IW656_000276"
     CDS             complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IW656_000276"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0836010.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43125..44492)
                     /locus_tag="IW656_000277"
     CDS             complement(43125..44492)
                     /locus_tag="IW656_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0836011.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44476..45306)
                     /locus_tag="IW656_000278"
     CDS             complement(44476..45306)
                     /locus_tag="IW656_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0836012.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45469..45906
                     /locus_tag="IW656_000279"
     CDS             45469..45906
                     /locus_tag="IW656_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0836013.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45982..46602
                     /locus_tag="IW656_000280"
     CDS             45982..46602
                     /locus_tag="IW656_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0836014.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            46791..47297
                     /locus_tag="IW656_000281"
     CDS             46791..47297
                     /locus_tag="IW656_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0836015.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            47314..48213
                     /locus_tag="IW656_000282"
     CDS             47314..48213
                     /locus_tag="IW656_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0836016.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            48300..49184
                     /locus_tag="IW656_000283"
     CDS             48300..49184
                     /locus_tag="IW656_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0836017.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(49376..49447)
                     /locus_tag="IW656_000284"
     tRNA            complement(49376..49447)
                     /locus_tag="IW656_000284"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49412..49414),aa:Arg,seq:ccg)
     gene            49507..50841
                     /gene="rpoN"
                     /locus_tag="IW656_000285"
     CDS             49507..50841
                     /gene="rpoN"
                     /locus_tag="IW656_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0836018.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            51044..52081
                     /locus_tag="IW656_000286"
     CDS             51044..52081
                     /locus_tag="IW656_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0836019.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            52122..53123
                     /gene="gap"
                     /locus_tag="IW656_000287"
     CDS             52122..53123
                     /gene="gap"
                     /locus_tag="IW656_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0836020.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            53233..54423
                     /locus_tag="IW656_000288"
     CDS             53233..54423
                     /locus_tag="IW656_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0836021.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            54527..55282
                     /locus_tag="IW656_000289"
     CDS             54527..55282
                     /locus_tag="IW656_000289"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0836022.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            55398..56696
                     /gene="eno"
                     /locus_tag="IW656_000290"
     CDS             55398..56696
                     /gene="eno"
                     /locus_tag="IW656_000290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0836023.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18898 a         9877 c        12009 g        16018 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa
     3001 tcattacact atcggacacc aatggaagta tttttgagtt acgtcacaaa agatgatttg
     3061 tctaacttaa tttgacaaac gaaacttaat aaaaaaggaa gaatattcat gtaaacgatt
     3121 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
     3181 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
     3241 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
     3301 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
     3361 accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
     3421 agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga
     3481 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
     3541 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
     3601 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
     3661 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
     3721 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
     3781 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
     3841 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
     3901 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
     3961 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
     4021 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
     4081 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
     4141 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
     4201 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
     4261 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
     4321 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
     4381 gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt
     4441 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
     4501 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
     4561 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
     4621 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
     4681 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
     4741 gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
     4801 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
     4861 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
     4921 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
     4981 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
     5041 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
     5101 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
     5161 tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa
     5221 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
     5281 gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
     5341 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
     5401 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
     5461 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
     5521 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
     5581 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
     5641 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
     5701 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
     5761 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
     5821 tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc
     5881 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg
     5941 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
     6001 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
     6061 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
     6121 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
     6181 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
     6241 caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat
     6301 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
     6361 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
     6421 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
     6481 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
     6541 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
     6601 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
     6661 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
     6721 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
     6781 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
     6841 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
     6901 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
     6961 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
     7021 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
     7081 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
     7141 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
     7201 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
     7261 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat
     7321 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
     7381 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
     7441 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
     7501 tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
     7561 ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc
     7621 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga
     7681 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
     7741 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
     7801 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
     7861 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
     7921 agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc
     7981 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
     8041 aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
     8101 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
     8161 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
     8221 tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
     8281 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
     8341 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
     8401 cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga
     8461 cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
     8521 ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata
     8581 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
     8641 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
     8701 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
     8761 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
     8821 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
     8881 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
     8941 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
     9001 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
     9061 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
     9121 gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta
     9181 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
     9241 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
     9301 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
     9361 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
     9421 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
     9481 tcgtgatcat tggtgcttcc catgaaggca tcacagctgc cctaaatcta agaaaacttc
     9541 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
     9601 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
     9661 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
     9721 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
     9781 cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg
     9841 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
     9901 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag
     9961 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    10021 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    10081 taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    10141 ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc
    10201 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    10261 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    10321 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    10381 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    10441 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    10501 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    10561 aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    10621 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    10681 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    10741 tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    10801 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    10861 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    10921 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    10981 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    11041 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    11101 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    11161 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    11221 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg
    11281 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    11341 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    11401 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag gagatacagt
    11461 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    11521 caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc gcttcgaaaa
    11581 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    11641 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    11701 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    11761 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    11821 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    11881 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    11941 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    12001 gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    12061 gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt taaagaagta
    12121 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    12181 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    12241 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    12301 gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga aattggcttt
    12361 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    12421 gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc
    12481 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    12541 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    12601 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    12661 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    12721 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    12781 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    12841 gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa aagttgggat
    12901 cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    12961 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    13021 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    13081 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    13141 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    13201 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    13261 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    13321 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    13381 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc tttatatagg
    13441 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    13501 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    13561 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    13621 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    13681 ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct tagtctggat
    13741 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    13801 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    13861 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    13921 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    13981 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    14041 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    14101 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    14161 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    14221 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    14281 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    14341 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    14401 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    14461 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    14521 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    14581 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    14641 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    14701 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt aaacaccgtt
    14761 taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa tttcaaaaaa
    14821 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    14881 gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa acaaacggaa
    14941 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga tgattgaaca
    15001 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    15061 gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    15121 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    15181 acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa aagctggcac
    15241 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    15301 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    15361 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    15421 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    15481 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    15541 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    15601 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga
    15661 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    15721 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    15781 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    15841 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    15901 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    15961 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    16021 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    16081 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    16141 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt
    16201 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    16261 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    16321 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    16381 aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    16441 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    16501 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    16561 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    16621 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    16681 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    16741 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    16801 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    16861 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    16921 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    16981 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    17041 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    17101 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    17161 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    17221 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    17281 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    17341 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    17401 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    17461 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    17521 acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    17581 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    17641 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    17701 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    17761 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    17821 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    17881 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    17941 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt tatttattga
    18001 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    18061 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    18121 catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    18181 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    18241 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    18301 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    18361 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    18421 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    18481 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    18541 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    18601 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    18661 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    18721 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    18781 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    18841 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    18901 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    18961 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    19021 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    19081 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    19141 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    19201 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    19261 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    19321 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    19381 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    19441 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    19501 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    19561 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    19621 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    19681 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    19741 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    19801 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    19861 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    19921 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    19981 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    20041 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    20101 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    20161 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    20221 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    20281 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    20341 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    20401 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    20461 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    20521 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    20581 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    20641 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    20701 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    20761 tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    20821 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    20881 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    20941 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    21001 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    21061 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    21121 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    21181 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    21241 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    21301 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    21361 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    21421 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    21481 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    21541 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    21601 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    21661 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg taaaaaacga
    21721 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    21781 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    21841 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    21901 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    21961 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    22021 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    22081 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    22141 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    22201 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    22261 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    22321 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    22381 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    22441 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    22501 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    22561 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    22621 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    22681 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    22741 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    22801 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    22861 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    22921 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    22981 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    23041 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    23101 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    23161 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    23221 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    23281 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    23341 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    23401 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    23461 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    23521 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    23581 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    23641 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    23701 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    23761 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    23821 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    23881 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    23941 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    24001 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    24061 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    24121 ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca gtcggtatgg
    24181 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    24241 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    24301 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    24361 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    24421 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    24481 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    24541 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    24601 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    24661 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    24721 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    24781 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    24841 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    24901 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    24961 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    25021 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    25081 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    25141 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    25201 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    25261 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    25321 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    25381 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    25441 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    25501 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    25561 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    25621 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    25681 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    25741 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    25801 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    25861 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    25921 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    25981 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    26041 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    26101 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    26161 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    26221 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    26281 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    26341 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    26401 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    26461 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    26521 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    26581 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    26641 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    26701 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    26761 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    26821 aggattttat gcatatcatc tttgctttga accgcttcaa cgagtccatc gttagaaaaa
    26881 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    26941 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    27001 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    27061 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    27121 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    27181 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    27241 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    27301 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    27361 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    27421 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    27481 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    27541 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    27601 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    27661 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    27721 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    27781 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    27841 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    27901 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    27961 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    28021 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    28081 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    28141 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    28201 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    28261 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    28321 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    28381 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    28441 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    28501 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    28561 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    28621 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    28681 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    28741 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    28801 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    28861 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    28921 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    28981 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    29041 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    29101 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    29161 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    29221 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    29281 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    29341 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    29401 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    29461 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    29521 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    29581 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    29641 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    29701 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    29761 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    29821 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    29881 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    29941 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    30001 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    30061 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    30121 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    30181 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    30241 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    30301 attctgcaat tgcttccagg cacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    30361 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    30421 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    30481 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    30541 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    30601 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    30661 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    30721 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    30781 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    30841 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    30901 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    30961 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    31021 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    31081 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    31141 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    31201 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    31261 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    31321 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    31381 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    31441 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    31501 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    31561 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    31621 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    31681 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    31741 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    31801 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    31861 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    31921 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    31981 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    32041 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    32101 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    32161 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    32221 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    32281 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    32341 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    32401 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    32461 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    32521 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    32581 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    32641 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    32701 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    32761 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    32821 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    32881 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    32941 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    33001 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    33061 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    33121 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    33181 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    33241 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    33301 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    33361 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    33421 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    33481 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    33541 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    33601 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    33661 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    33721 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    33781 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    33841 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    33901 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    33961 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    34021 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    34081 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    34141 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    34201 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    34261 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    34321 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    34381 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    34441 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    34501 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    34561 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    34621 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    34681 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    34741 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    34801 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    34861 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    34921 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    34981 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    35041 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    35101 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    35161 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    35221 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    35281 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    35341 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    35401 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    35461 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    35521 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    35581 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    35641 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    35701 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    35761 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    35821 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    35881 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    35941 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    36001 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    36061 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    36121 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    36181 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    36241 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    36301 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    36361 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    36421 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    36481 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    36541 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    36601 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    36661 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    36721 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    36781 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    36841 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    36901 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    36961 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    37021 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    37081 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    37141 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    37201 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    37261 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    37321 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    37381 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    37441 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    37501 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    37561 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    37621 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    37681 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    37741 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    37801 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    37861 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    37921 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    37981 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    38041 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    38101 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    38161 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    38221 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    38281 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    38341 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    38401 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    38461 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    38521 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    38581 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    38641 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    38701 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    38761 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    38821 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    38881 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    38941 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    39001 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    39061 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    39121 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    39181 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    39241 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    39301 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    39361 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    39421 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    39481 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    39541 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    39601 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    39661 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    39721 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    39781 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    39841 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    39901 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    39961 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    40021 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    40081 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    40141 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    40201 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    40261 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    40321 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    40381 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    40441 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    40501 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    40561 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    40621 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    40681 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    40741 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    40801 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    40861 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    40921 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    40981 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    41041 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    41101 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    41161 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    41221 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    41281 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    41341 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    41401 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    41461 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    41521 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    41581 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    41641 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    41701 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    41761 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    41821 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    41881 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    41941 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    42001 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    42061 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    42121 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    42181 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    42241 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    42301 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    42361 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    42421 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    42481 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    42541 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    42601 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    42661 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    42721 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    42781 gaatggagat gactgccgac acggcgtgcc attacttcga tctctaggaa atcttcatgt
    42841 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    42901 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    42961 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    43021 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    43081 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    43141 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    43201 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    43261 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    43321 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    43381 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    43441 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    43501 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    43561 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    43621 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    43681 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    43741 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    43801 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    43861 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    43921 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    43981 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    44041 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    44101 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    44161 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    44221 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    44281 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    44341 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    44401 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    44461 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    44521 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    44581 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    44641 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    44701 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    44761 tcgcttcatg acttgcccac gtttttcctg attaccgttg acacttaagt acatcataac
    44821 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    44881 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    44941 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    45001 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    45061 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatcttcca gtgtttggat
    45121 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    45181 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    45241 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    45301 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    45361 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    45421 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    45481 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    45541 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    45601 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    45661 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    45721 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    45781 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    45841 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    45901 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    45961 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    46021 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    46081 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    46141 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    46201 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    46261 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    46321 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    46381 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    46441 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    46501 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    46561 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    46621 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    46681 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    46741 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    46801 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    46861 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    46921 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    46981 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    47041 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    47101 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    47161 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    47221 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    47281 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    47341 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    47401 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    47461 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    47521 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    47581 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    47641 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    47701 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    47761 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    47821 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    47881 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    47941 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    48001 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    48061 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    48121 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    48181 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    48241 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    48301 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    48361 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    48421 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    48481 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    48541 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    48601 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    48661 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    48721 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    48781 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    48841 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    48901 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    48961 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    49021 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    49081 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    49141 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    49201 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    49261 aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt
    49321 tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt
    49381 cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta
    49441 aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag gggattggac
    49501 aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc
    49561 atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg
    49621 gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg
    49681 cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta
    49741 aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg
    49801 aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg
    49861 aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc
    49921 caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt
    49981 agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct
    50041 tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca
    50101 caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg
    50161 agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc
    50221 cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca
    50281 aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact
    50341 gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa
    50401 cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt
    50461 atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat
    50521 gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt
    50581 gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca
    50641 gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac
    50701 aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg
    50761 atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa
    50821 agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag
    50881 tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt
    50941 ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt
    51001 taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa
    51061 aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt
    51121 acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat
    51181 cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac
    51241 ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa
    51301 cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg
    51361 taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc
    51421 attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat
    51481 cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa
    51541 taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt
    51601 acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact
    51661 ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat
    51721 ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc
    51781 attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc
    51841 aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa
    51901 ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat
    51961 cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg caccaaaaca
    52021 aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta
    52081 aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta
    52141 ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa
    52201 tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat
    52261 acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg
    52321 taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg
    52381 aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg
    52441 aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg
    52501 ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct
    52561 caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat
    52621 ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc
    52681 tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg
    52741 ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta
    52801 aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa
    52861 cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa
    52921 acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt
    52981 acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag
    53041 ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt
    53101 tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta
    53161 catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca
    53221 ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt
    53281 cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt
    53341 atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt
    53401 ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca
    53461 gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc
    53521 ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa
    53581 aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt
    53641 aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt
    53701 gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg
    53761 atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt
    53821 gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct
    53881 aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga
    53941 atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc
    54001 gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca
    54061 aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac
    54121 atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt
    54181 tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt
    54241 tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagct
    54301 gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac aggtggtggc
    54361 gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa
    54421 taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa gggagttttc
    54481 atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat
    54541 tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc
    54601 agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt
    54661 attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa
    54721 ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga
    54781 tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac
    54841 agacgaagac atcaataaaa aagcaaaagc tatctttgca aatggtatga caccaatcct
    54901 atgctgcggc gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg
    54961 acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca tggttattgc
    55021 ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga
    55081 aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc
    55141 agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa
    55201 agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt catttttgtc
    55261 attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca
    55321 ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact
    55381 caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact
    55441 cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg
    55501 gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag
    55561 acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca
    55621 ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga
    55681 tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt
    55741 ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag
    55801 gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac
    55861 atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat
    55921 tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag
    55981 ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa
    56041 acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta
    56101 aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg
    56161 tttacgtact agctgactca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct
    56221 acgaagaatt agtttctaaa tatccaatca tctcaatcga agatggatta gacgaaaacg
    56281 actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg
    56341 acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta
    56401 actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg
    56461 aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag
    56521 attcaacaat ctctgatatc gctgttgcaa caaatgctgg ccaaatcaaa actggttctc
    56581 tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg
    56641 gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct
    56701 tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa
    56761 gtaatcgaac tctctacttg ctgaaatatc agcaggtaga ga
//
LOCUS       DACEJZ010000005        56347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEJZ010000005 DACEJZ010000000
VERSION     DACEJZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.005"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            290..1135
                     /locus_tag="IW656_000291"
     CDS             290..1135
                     /locus_tag="IW656_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0836024.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IW656_000292"
     CDS             1416..2636
                     /locus_tag="IW656_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0836025.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IW656_000293"
     CDS             2633..5353
                     /locus_tag="IW656_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0836026.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IW656_000294"
     CDS             5337..6281
                     /locus_tag="IW656_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0836027.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IW656_000295"
     CDS             complement(6350..7360)
                     /locus_tag="IW656_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0836028.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IW656_000296"
     CDS             complement(7540..7884)
                     /locus_tag="IW656_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0836029.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IW656_000297"
     CDS             complement(7877..8311)
                     /locus_tag="IW656_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0836030.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IW656_000298"
     CDS             8449..9186
                     /locus_tag="IW656_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836031.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IW656_000299"
     CDS             9179..10390
                     /locus_tag="IW656_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836032.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IW656_000300"
     CDS             10597..11376
                     /locus_tag="IW656_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0836033.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IW656_000301"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IW656_000301"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0836034.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW656_000302"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW656_000302"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0836035.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IW656_000303"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IW656_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0836036.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IW656_000304"
     CDS             14978..16027
                     /locus_tag="IW656_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0836037.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IW656_000305"
     CDS             16024..16302
                     /locus_tag="IW656_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0836038.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IW656_000306"
     CDS             16277..16747
                     /locus_tag="IW656_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0836039.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IW656_000307"
     CDS             16764..17066
                     /locus_tag="IW656_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836040.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IW656_000308"
     CDS             17083..17454
                     /locus_tag="IW656_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0836041.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IW656_000309"
     CDS             17426..17785
                     /locus_tag="IW656_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836042.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IW656_000310"
     CDS             17957..18964
                     /locus_tag="IW656_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0836043.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IW656_000311"
     CDS             18989..20173
                     /locus_tag="IW656_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0836044.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IW656_000312"
     CDS             complement(20610..22193)
                     /locus_tag="IW656_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0836045.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IW656_000313"
     CDS             complement(22771..22914)
                     /locus_tag="IW656_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0836046.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IW656_000314"
     CDS             complement(23137..23616)
                     /locus_tag="IW656_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0836047.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IW656_000315"
     CDS             complement(23689..23973)
                     /locus_tag="IW656_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836048.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IW656_000316"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW656_000316"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IW656_000317"
     CDS             complement(24317..25603)
                     /locus_tag="IW656_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0836049.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IW656_000318"
     CDS             25741..26412
                     /locus_tag="IW656_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0836050.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IW656_000319"
     CDS             26378..26866
                     /locus_tag="IW656_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0836051.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IW656_000320"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IW656_000320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0836052.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IW656_000321"
     CDS             27827..28591
                     /locus_tag="IW656_000321"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0836053.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IW656_000322"
     CDS             28824..31037
                     /locus_tag="IW656_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0836054.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKEKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IW656_000323"
     CDS             31048..31494
                     /locus_tag="IW656_000323"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0836055.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IW656_000324"
     CDS             31665..32924
                     /locus_tag="IW656_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0836056.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IW656_000325"
     CDS             complement(33013..33960)
                     /locus_tag="IW656_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0836057.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IW656_000326"
     CDS             34354..35646
                     /locus_tag="IW656_000326"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0836058.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IW656_000327"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IW656_000327"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0836059.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IW656_000328"
     CDS             37539..38414
                     /locus_tag="IW656_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0836060.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IW656_000329"
     CDS             38680..40563
                     /locus_tag="IW656_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0836061.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IW656_000330"
     CDS             complement(40638..40922)
                     /locus_tag="IW656_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0836062.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IW656_000331"
     CDS             complement(40912..41646)
                     /locus_tag="IW656_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0836063.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IW656_000332"
     CDS             41775..42398
                     /locus_tag="IW656_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0836064.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IW656_000333"
     CDS             42590..43711
                     /locus_tag="IW656_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0836065.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IW656_000334"
     CDS             43718..46846
                     /locus_tag="IW656_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0836066.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IW656_000335"
     CDS             46865..50059
                     /locus_tag="IW656_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0836067.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IW656_000336"
     CDS             complement(50205..50912)
                     /locus_tag="IW656_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0836068.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="IW656_000337"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="IW656_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0836069.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="IW656_000338"
     CDS             52407..53060
                     /locus_tag="IW656_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0836070.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="IW656_000339"
     CDS             53286..54284
                     /locus_tag="IW656_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0836071.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="IW656_000340"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="IW656_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0836072.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18539 a         9724 c        11679 g        16405 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagaa aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DACEJZ010000006        53454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEJZ010000006 DACEJZ010000000
VERSION     DACEJZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.006"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..599)
                     /gene="nrdG"
                     /locus_tag="IW656_000341"
     CDS             complement(<1..599)
                     /gene="nrdG"
                     /locus_tag="IW656_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0836073.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDL"
     gene            complement(914..3103)
                     /gene="nrdD"
                     /locus_tag="IW656_000342"
     CDS             complement(914..3103)
                     /gene="nrdD"
                     /locus_tag="IW656_000342"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0836074.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            3429..4196
                     /locus_tag="IW656_000343"
     CDS             3429..4196
                     /locus_tag="IW656_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836075.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            4186..6228
                     /locus_tag="IW656_000344"
     CDS             4186..6228
                     /locus_tag="IW656_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836076.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            6509..6658
                     /locus_tag="IW656_000345"
     CDS             6509..6658
                     /locus_tag="IW656_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0836077.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            6669..8183
                     /gene="dltA"
                     /locus_tag="IW656_000346"
     CDS             6669..8183
                     /gene="dltA"
                     /locus_tag="IW656_000346"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0836078.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            8180..9385
                     /gene="dltB"
                     /locus_tag="IW656_000347"
     CDS             8180..9385
                     /gene="dltB"
                     /locus_tag="IW656_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0836079.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            9430..9663
                     /gene="dltC"
                     /locus_tag="IW656_000348"
     CDS             9430..9663
                     /gene="dltC"
                     /locus_tag="IW656_000348"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0836080.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            9666..10934
                     /gene="dltD"
                     /locus_tag="IW656_000349"
     CDS             9666..10934
                     /gene="dltD"
                     /locus_tag="IW656_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0836081.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            11075..12157
                     /locus_tag="IW656_000350"
     CDS             11075..12157
                     /locus_tag="IW656_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0836082.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            12157..12960
                     /locus_tag="IW656_000351"
     CDS             12157..12960
                     /locus_tag="IW656_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0836083.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFVFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            13030..13770
                     /locus_tag="IW656_000352"
     CDS             13030..13770
                     /locus_tag="IW656_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0836084.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            13781..14785
                     /locus_tag="IW656_000353"
     CDS             13781..14785
                     /locus_tag="IW656_000353"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0836085.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(14830..16674)
                     /locus_tag="IW656_000354"
     CDS             complement(14830..16674)
                     /locus_tag="IW656_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836086.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            16886..17404
                     /locus_tag="IW656_000355"
     CDS             16886..17404
                     /locus_tag="IW656_000355"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0836087.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            17532..18215
                     /locus_tag="IW656_000356"
     CDS             17532..18215
                     /locus_tag="IW656_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0836088.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            18524..18597
                     /locus_tag="IW656_000357"
     tRNA            18524..18597
                     /locus_tag="IW656_000357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18558..18560,aa:Arg,seq:tct)
     gene            18756..19115
                     /locus_tag="IW656_000358"
     CDS             18756..19115
                     /locus_tag="IW656_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0836089.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            19238..20869
                     /locus_tag="IW656_000359"
     CDS             19238..20869
                     /locus_tag="IW656_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0836090.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(20931..21572)
                     /locus_tag="IW656_000360"
     CDS             complement(20931..21572)
                     /locus_tag="IW656_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0836091.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            21854..22162
                     /gene="rplU"
                     /locus_tag="IW656_000361"
     CDS             21854..22162
                     /gene="rplU"
                     /locus_tag="IW656_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0836092.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            22189..22533
                     /locus_tag="IW656_000362"
     CDS             22189..22533
                     /locus_tag="IW656_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0836093.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            22546..22839
                     /gene="rpmA"
                     /locus_tag="IW656_000363"
     CDS             22546..22839
                     /gene="rpmA"
                     /locus_tag="IW656_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0836094.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(22932..24080)
                     /locus_tag="IW656_000364"
     CDS             complement(22932..24080)
                     /locus_tag="IW656_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0836095.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(24092..24403)
                     /locus_tag="IW656_000365"
     CDS             complement(24092..24403)
                     /locus_tag="IW656_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836096.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(24456..24851)
                     /locus_tag="IW656_000366"
     CDS             complement(24456..24851)
                     /locus_tag="IW656_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0836097.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(24887..25231)
                     /locus_tag="IW656_000367"
     CDS             complement(24887..25231)
                     /locus_tag="IW656_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0836098.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            25532..25753
                     /locus_tag="IW656_000368"
     CDS             25532..25753
                     /locus_tag="IW656_000368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836099.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            25880..26656
                     /locus_tag="IW656_000369"
     CDS             25880..26656
                     /locus_tag="IW656_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0836100.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            26653..26847
                     /locus_tag="IW656_000370"
     CDS             26653..26847
                     /locus_tag="IW656_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0836101.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            26849..27133
                     /locus_tag="IW656_000371"
     CDS             26849..27133
                     /locus_tag="IW656_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836102.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            27154..27342
                     /locus_tag="IW656_000372"
     CDS             27154..27342
                     /locus_tag="IW656_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836103.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            27668..27907
                     /locus_tag="IW656_000373"
     CDS             27668..27907
                     /locus_tag="IW656_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836104.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            27913..28599
                     /locus_tag="IW656_000374"
     CDS             27913..28599
                     /locus_tag="IW656_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836105.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            28602..29432
                     /locus_tag="IW656_000375"
     CDS             28602..29432
                     /locus_tag="IW656_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0836106.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            29449..30300
                     /locus_tag="IW656_000376"
     CDS             29449..30300
                     /locus_tag="IW656_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0836107.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            30297..30656
                     /locus_tag="IW656_000377"
     CDS             30297..30656
                     /locus_tag="IW656_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0836108.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            30671..30832
                     /locus_tag="IW656_000378"
     CDS             30671..30832
                     /locus_tag="IW656_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0836109.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            30829..31134
                     /locus_tag="IW656_000379"
     CDS             30829..31134
                     /locus_tag="IW656_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836110.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            31134..31490
                     /locus_tag="IW656_000380"
     CDS             31134..31490
                     /locus_tag="IW656_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0836111.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            31450..31695
                     /locus_tag="IW656_000381"
     CDS             31450..31695
                     /locus_tag="IW656_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836112.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            31716..31805
                     /locus_tag="IW656_000382"
                     /pseudo
     CDS             31716..31805
                     /locus_tag="IW656_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            31880..32347
                     /locus_tag="IW656_000383"
     CDS             31880..32347
                     /locus_tag="IW656_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAQ0836113.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            32622..32858
                     /locus_tag="IW656_000384"
     CDS             32622..32858
                     /locus_tag="IW656_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836114.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            32882..33223
                     /locus_tag="IW656_000385"
     CDS             32882..33223
                     /locus_tag="IW656_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836115.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(33216..33395)
                     /locus_tag="IW656_000386"
     CDS             complement(33216..33395)
                     /locus_tag="IW656_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ0836116.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            33617..34264
                     /locus_tag="IW656_000387"
     CDS             33617..34264
                     /locus_tag="IW656_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836117.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            34524..34868
                     /locus_tag="IW656_000388"
     CDS             34524..34868
                     /locus_tag="IW656_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0836118.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            34873..35154
                     /locus_tag="IW656_000389"
     CDS             34873..35154
                     /locus_tag="IW656_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836119.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            35257..35571
                     /locus_tag="IW656_000390"
     CDS             35257..35571
                     /locus_tag="IW656_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0836120.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            35549..37243
                     /locus_tag="IW656_000391"
     CDS             35549..37243
                     /locus_tag="IW656_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0836121.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            37263..38441
                     /locus_tag="IW656_000392"
     CDS             37263..38441
                     /locus_tag="IW656_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0836122.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            38404..39090
                     /locus_tag="IW656_000393"
     CDS             38404..39090
                     /locus_tag="IW656_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0836123.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            39090..40250
                     /locus_tag="IW656_000394"
     CDS             39090..40250
                     /locus_tag="IW656_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0836124.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            40260..41135
                     /locus_tag="IW656_000395"
     CDS             40260..41135
                     /locus_tag="IW656_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836125.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            41132..41443
                     /locus_tag="IW656_000396"
     CDS             41132..41443
                     /locus_tag="IW656_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836126.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            41433..41786
                     /locus_tag="IW656_000397"
     CDS             41433..41786
                     /locus_tag="IW656_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0836127.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            41776..42177
                     /locus_tag="IW656_000398"
     CDS             41776..42177
                     /locus_tag="IW656_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836128.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            42170..42574
                     /locus_tag="IW656_000399"
     CDS             42170..42574
                     /locus_tag="IW656_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836129.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            42586..43191
                     /locus_tag="IW656_000400"
     CDS             42586..43191
                     /locus_tag="IW656_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0836130.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            43211..43573
                     /locus_tag="IW656_000401"
     CDS             43211..43573
                     /locus_tag="IW656_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836131.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            43576..43758
                     /locus_tag="IW656_000402"
     CDS             43576..43758
                     /locus_tag="IW656_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836132.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            43775..47194
                     /locus_tag="IW656_000403"
     CDS             43775..47194
                     /locus_tag="IW656_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0836133.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            47245..47982
                     /locus_tag="IW656_000404"
     CDS             47245..47982
                     /locus_tag="IW656_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0836134.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            47992..50283
                     /locus_tag="IW656_000405"
     CDS             47992..50283
                     /locus_tag="IW656_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836135.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            50307..52397
                     /locus_tag="IW656_000406"
     CDS             50307..52397
                     /locus_tag="IW656_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836136.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            52560..53006
                     /locus_tag="IW656_000407"
     CDS             52560..53006
                     /locus_tag="IW656_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836137.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            53008..53145
                     /locus_tag="IW656_000408"
     CDS             53008..53145
                     /locus_tag="IW656_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0836138.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            53183..>53454
                     /locus_tag="IW656_000409"
     CDS             53183..>53454
                     /locus_tag="IW656_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836139.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT        18057 a         8448 c        10805 g        16144 t
ORIGIN      
        1 atcaagtctt gctttttgat ttgttcatag gtttctgttg catccgtaca tttttcccaa
       61 atcacaactt gattggattc taaagatttt tggacgtcaa tgatccgctg gttgctactt
      121 ccgcgaaatt gtagttttag gtcacgtttg cttaattcaa agcgtccgtc aacaaggata
      181 tcgatttgcg aaaggagttc taatttatct tctgaatctt gtagcaattc atcaaaggta
      241 tagcctgtcc atgaccagat atcttttgtc gtaccgaact cttttcggat acgtcgtacg
      301 acttgcagac aaacatctgt attcaaaaaa ggttcgccac caagtaaagt taatccttgc
      361 acgtaatcgt tgcgcaagtc agcgatgatt tgttcttcta attcaggtgt gaatggtttg
      421 ccatatcgga aattttgtgc agctttgttg aagcagcctt cgcaggcaaa taagcagcca
      481 ctaacgtaaa gactgttgcg gacaccttca ccatcgacaa aattaaacgg tttgtagtca
      541 gcaatatagt tttggctgta gtcttttgct aaccattctt ttggttttgg atttctcata
      601 aaaaaacttc tttcatttaa ggttcaaaga tatagctatt gaagataaaa tctactcgac
      661 cacataaata agaacaaact gacctaaatc cattgttcaa gaaagcttgt tcttattttg
      721 atagagggcg ttcaaagata caaagccatc gaaagagggt ggtgaaaaaa gacttgtcat
      781 agcctcttat tctgaataaa cggtgttcaa aagcttgcat ctgcagtaat aagcccaact
      841 aaacgaaaaa tatgaggaac tatttttgtt tagctattct tattacttga tgcaggacag
      901 ctttttcaca acctcatttc atgtgtttca cacgtgagga aatttcttta tggcggccat
      961 gtaccatcgg acgagcttgc ggatttccta aatagccaca agtacgtttg actacatcac
     1021 atgtttttgg atcatgattt ccacattctg gacattcgaa ccctcgttct gtcggtttga
     1081 aatcaccttc gaaaccacat tgatagcaat gatcgattgg cgtattcgta ccaagatacc
     1141 cgattttgtc atatgcaaaa tcccatacag cttctaaagc tttcggattt tgttggagca
     1201 tagggtattc acagtagtgg atgaatccac ctgaacaata ttttggataa tccttctcaa
     1261 attccaattt ttcaaatggc gttgggtttt tacgcacatc atagtggaaa ctgtttgtat
     1321 aatattcttt atctgtgata ttttcgatga tgccgaattt ttctgtatcc aaacggcaga
     1381 aacggtcagt tagactttca cttggagttg aatagacact gaaatgataa ccatattcat
     1441 ccccccatgc atctgcatta gcttttaatt ctttgacgat atcaagtgtg aaagcttttg
     1501 cttctgggtt tgtttcccat tcgccaccga agaaagctga agcaacttca tacaatccaa
     1561 tgtagcctaa cgatacagta gctcgacggt ttttgaataa ttcattgaca gaatcggtac
     1621 gtgaaagacg tttcccgaaa gcaccataca tatataaaat tggcgcattt gctggaatgg
     1681 cttctttaca acgttctaca cgatagagaa gcgcatcttt catgatttct aagcggtcag
     1741 acagtaactg ccagaattta ttctgatctc cttttgattc gatcgcgatt cttggcaaat
     1801 tcaatgtaac aacacctaaa ttcattcgtc cgacatttac ttcttgacca ttttcgtctt
     1861 tccacccttg caagaatgaa cgacagccca taggtacttt gaaacttcca gttaattcaa
     1921 cgattttatc gtaattcaga atatcaggat acattctttt cgttgcacat tccaatgcta
     1981 gttgtttgat gtcgtaatta ggatcttcag ggtttaagtt cacacctttt ttcaaagtga
     2041 agatcagttt agggaagata gctgtccgct tttcactccc taatccattg attcgaatct
     2101 gtaaaattgc tttttgaatt tctctttcaa accagttttc tcctagtcca aatcctaatg
     2161 aagtgaaagg agtttgtcca tttgaagtaa ataaggtatt gatttcatat tccaaacttt
     2221 gcatagcatc gaagatatct tttttggttt ttgcttttgc atattctttt tggcgttctg
     2281 gcgaatcgat ccattcttcc gcgtctttta gatgtttttt atagttcaat tctgcaaaag
     2341 gagcaagcaa ttcatctgtt cggtcggcag aacagccacc atattgactt gacgctacat
     2401 tggcaataat ctgtgagatt tgtgctgtgg cggtctggat agattttggc gattcgactt
     2461 cggcattgcc gattttgaac ccattgttca gcatgccttt gaaatcaatc aagcagcaat
     2521 tcgtcatcgg tgtataagga tgataatcca aatcatggta atggatatcg cctttttgat
     2581 gcgcatttgc tatatgtggt ggcaacattt tcagtccgat tgattttcca acgatcccgg
     2641 ctgtcagatc tcgctgtgta ttgaaaacat tactgtcttt atttgcgttt tcgttcacga
     2701 cagtttggtc cttgttgatt agcttttcaa tcgaaaagtt gatatctgtt gctttactgc
     2761 gagcaaaatc tcgtttggta cgataattga tgtactcttc tgctaattca tattcgtttt
     2821 tttctagtaa tatatgttcc acgatgtttt gaatctcata gattttgaca ttttcagcaa
     2881 accggctttt gatttcttgg acaaccaagt ctgcaatcat ttgaattttt tcataagtaa
     2941 gtggtgataa tgagccgtga attttctttt ctgctttgat cagtgcttca ttgatctttt
     3001 gttcatcgaa atcaacatgg cggccatcgc gtttgatgac tttcatctgg gatgtctctg
     3061 gctgctgagt aacgacagtt ttttcttcat aatttacttt cattatcttc aactcctgct
     3121 aaattgttct tgtctatcat aaaacaaaat cggggaagga gcaactaaat atcggctata
     3181 tatagtattc aatgtgctgt ataattggat ttctatacta tatgtagtgt atattttatt
     3241 gtgaaaaaag gtgaaaactt gtagctggtg gatttgtttt ttgcatattt aattttgctg
     3301 aataaaataa ttgaataaaa ttttttattc agtccaagta aaagtgacaa aaacataaga
     3361 gcaaattatt cgtagatatg ctactatgca aataaataga aatgaaaaat gaaagaggag
     3421 gagaagaatt ggaaaagatg atcgaagtga aacatctaag taaaagctac ggcagcaaac
     3481 tttctcccgt caaagcctta gataatatct cattcacagt agaagaaggc gagtttgttg
     3541 ggattatggg cccaagtggt gcaggaaaga caacgctgat gaatatcttg tccacgatca
     3601 atttgcctac gatgggcagt gtctcgatcc aaggaaaaga aatcacaaaa atgaaaaaca
     3661 gtgagctcag tgactttcgt cgaaaaaaac taggattcat ttttcaagag ttcaatttga
     3721 tcgataccct aaatgcaaaa gacaatatcc tattgcccct agcagttgaa cggatgacta
     3781 aagaggaaat ggaaaagaga gtaagacatg tagctcaatt gttgaatata gaagaactgc
     3841 tcaaacggta tcccgatgaa ctttctgtcg gacagagaca acgaattgca gctgctagag
     3901 cacttgttgt tcagccacaa gtgatttttg cagatgaacc aactggctct ttggactcga
     3961 agtcggctac agaattgttg aactacttga agaccatgaa tcaaaaagaa aaagcaacga
     4021 tactgttagt cacccatgat ccatatacag caagctactg cgatagaatt ttatttatta
     4081 aagatggtgt gattttttct gaagttgtca gaagagggac aagaagagag ttctttgaga
     4141 aagtaataga catgcaggcg acgattggcg gaggaggaaa aatgaatgct gtataaacta
     4201 gccgttaaaa gtttttttcg gcagtcacga ggctatcttg tttatttttt cagtttgact
     4261 ttatccgtaa tgatctatta ttccttcagc gcaatgacct acgatcaatc tttagtcaga
     4321 agagcaagtc aagatacttc gatagatggt ggattgagtt ttggtggatt tatcattact
     4381 gttgttttgc tttttttcgt attcagtgcc aaccgatttt tcttgaatag aaggcaaaag
     4441 gaaataggca tctatcagct ttttggtatg aataaactgc agatatcagg tatatatgtg
     4501 ttagaaatca tgataattgg attgttcgcc tgtatttctg ggatattact cggtattatt
     4561 ttttccaaac tcttttcaat gattttggtt cgaatgatgg atatggatct tactagtcca
     4621 ttttttatct ccattccatc agtagtagat actttatttg cttttttcat catcctttta
     4681 gcagtatctg tatattcaat atggaaaata tggcgctatc caatgattcg aagttttgga
     4741 gaaaaagaac aagcagatag ttcaacaatg cgcttcagga caaggcaccg attactcgca
     4801 gtcatcgggt tactactttt aacgagcgga tattttggag ctagtcattt tagggaaatc
     4861 acttcatgga tgatcagtta tcattatctt gatacattag ttttcttttt tcctttttta
     4921 atttttttcg tttgtgtggt cggtacttat ttgtttttct gttatacatt tcggttattt
     4981 ttgatcatat tgagtaaatg gaagatttat tactatggta tcaatttttt gataatgggt
     5041 aacacgcaag ttcatttgct caaaagttgg cgtacaaatt ctctgattac agtcattctc
     5101 ggaatttctt taatgacaat tggcggcacg gctagtatct ctactatttt atcctaccag
     5161 gagaaagttt cctccccgat ggactatcag tttgatgttg agacagagaa aaaaatcaag
     5221 ccgattctag cagaggaagg gcagaagata actaaagaaa tcaccttgca ttataaagtc
     5281 attgggagta tctatagtca aaatctcgaa gaacctatgt ttcaaactgc taatctgatc
     5341 acggaaacac aatatcaagc cttcagaaaa gttaatccag atttgccgaa catttctttg
     5401 aaatcacctg gtcatactgt tttgcttgat caaatgcaaa ctctttttcg gaatatttca
     5461 ttatatggat ctagtattgg actgccagat aatcaagaat tgaagattca acaaatgctt
     5521 ccaagtgtat taggtgacga aacaatggta tatctaagtc ctgtactgat tgtagatgat
     5581 accgtttttg aaaaagcaaa aggtgtggat tatcaagtag tcaatgtaga tgtatcaggt
     5641 ggaaataatg aaaaaataga tgaacgactg agcaatgacc tgtcgataaa gtggctcaat
     5701 gctgtttatt attcttatga tatcgtaaat ggaagtctac aaggagaaat tagcacaaag
     5761 aaacttccgg atgaccaaat gaaggaagca caatttagcc aagaaagctc tcgtttgaat
     5821 ttctcttcca gctattcgaa attgcgtgca gttaatcggc gaatcggtat caatattttt
     5881 gtcacactat ttgtcgggat gatcgtgatt attgctactg gtagtatttt gacagtccgg
     5941 caattagcag aagcagagga agaaaaagaa aattatcacc tattgactaa gctggggatt
     6001 ccacaaagaa ggatcagacg gatgatcttc gagcaaaacg cacttacctt ctttccaccc
     6061 atgatattag ggatcactca cgcagtattt gcaatcaatg tcttcactca gtatataaaa
     6121 actgccgatt actggttggc ttatcttgtg agtggcttat tgatccttat ctatcttttg
     6181 ctttatgtca ttacgagtca gttatattgt cggattatcg aggaatgata tacggaataa
     6241 gacaagataa taaatagaac taaattttga ataccattta tttggaataa gaggttgtga
     6301 cacgattttt gtcacaacct ctttattttt tatatttttt aaaagtacga gaaattataa
     6361 agaaaaaagc agtgatttga tgtgctattt ttataaaagc tatgtgacaa aactgttagt
     6421 aaaaaaacgg tttctgtaag ctagacacat gtttcttttt gtcaaaatta gttacacttt
     6481 gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc caaaattgaa
     6541 ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc tgttcctgat
     6601 ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg aattttagga
     6661 ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga ataaatgaac
     6721 ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg aaacaagcat
     6781 cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca attgtcgttt
     6841 ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa tctggccatg
     6901 cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc tttcgggtgg
     6961 ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct tcggttcctg
     7021 tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt tatcccagaa
     7081 atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact actggagaac
     7141 caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg acgatggaag
     7201 actttgggat caagcctggt atgcatttct tagcccaagc gccattttct tttgatttat
     7261 cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg ttaaataaag
     7321 cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag ttgaatgtgt
     7381 gggtttctac tccatcattt atggatattt gcttgatgga acctacattc aatggagaaa
     7441 atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa aaaactgcag
     7501 aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt ccaacagaag
     7561 caactgtagc tatttcaagt attcagattg accagcatgt actagatagt tatgaccgac
     7621 taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa ggtaaacctg
     7681 tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca aaaggttacc
     7741 tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta gcaagttacc
     7801 atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa ggacgtatgg
     7861 atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa cattatctgc
     7921 tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga acaaaagtac
     7981 agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat tttcagttga
     8041 ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt cctcagaagt
     8101 tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga aaaaaattaa
     8161 cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag agccgtttta
     8221 tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga tatgggaaaa
     8281 aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca gctttggagg
     8341 taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt
     8401 atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt
     8461 tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg aacatggaaa
     8521 agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg tacaaatgat
     8581 catggaaata agagatggca tgatcaaaac atataagcca tttcgttata tccagtttct
     8641 gctgttcttt ccaacaatct cttctggacc aatcgatcgc tataggagat ttgaaaaaga
     8701 tcagctccat ccgcctgttc gtgataaata tgttgatctt ttgggaaaag gcgtccaaaa
     8761 tatttttgtc ggttttttat ataaattcat tatcggttat tattttggtc aagtactgct
     8821 tcctgtagtg ggacgcattg ccttacagca tggtggtatt tcatgggcat tagtaggtta
     8881 tatgtacgtg tacagtatgt atttgttttt tgattttgct ggctatagct tatttgcagt
     8941 cggtacaagc tacatgatgg gatatcagac accaatcaat ttcaataaac cttttttatc
     9001 atggaatatc aaagaattct ggaatcgttg gcatatgacg ctgtcctttt ggttccgtga
     9061 ttatgtatat atgcgattga tgttcttcat gatgaagaaa aaatttttta aaagcaagat
     9121 cgtaatgtca aatatcggtt attttgcttt gtttcttcta atgggtatat ggcatggatt
     9181 gacgtggtac tacatcgtat atggactgta tcatgcttgt ctgatctgtc tgacagatgc
     9241 atggatccga tttaagaaga agcataaaga gcagttgcct tctaacaaat ttacgcatgg
     9301 cttagccgta tttttgacat ttcaagcagt atgttttagc ttcctgattt tttcaggatt
     9361 tttagataaa ctttttttta gctaacgaat agtgacatat taatttatat aacaaaggag
     9421 aaaaggaaaa tggaagaaca aattttatca atcttagaag aaataacagg aacagatgaa
     9481 gtaagaaaaa atcgtgatat tgatctattt gatgaagggt tgcttgattc tttagcatct
     9541 gttcaattat tagtcgagct agacggagag ctggatatcc aagtccctgt atctgaattc
     9601 gagcgagagg attggagcac accgaataaa atcattcaac aagcaactgc attgaagggt
     9661 tgagtatgaa aaagaaactt tttgctattt tcggcccggt tgttttagct gttggcttac
     9721 tgtttgtgtt ttttacctcc tcctatcaag tagatatctc taatccagaa acgattagac
     9781 gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa aattatgcat
     9841 tatctgaaaa acaatatatt cctttttttg gctcaagtga attaagcaga atcagtccat
     9901 ttcatccgtc tgttctggca gaaaaatacc agcgaaacta tcgtccattt ttgctgggag
     9961 cacccgggac acaatcgtta agccaatata tgatgatgcg ttctgcagga gatgctatga
    10021 aaaacaagaa agtcgtcttt attatttctc ctcagtggtt cgttaaaaat ggtgtaaaaa
    10081 ctgattactt taatacgtat tattctgaac ttcaaacata cgactggttg ttttcaatga
    10141 agaaagtaac gcctgctgat cgctatttag ctagaagatt attaactttc tcaaaagtaa
    10201 aagagaatga tacgttgacg gcgattctgc aaacgatcaa aaaagggaag ctacctttac
    10261 ctgaatcttt gaatcagctg cgcagccaat ggaacatgct gaagagagag gatgaagtat
    10321 tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca aaacgattac
    10381 caaaacaata ccaagaaaca gaattatcca ttttagcaaa tcaaattgga gaacgagaaa
    10441 cgacgaataa tccattcggg ttgaaaaacg atttttatac gcatcgtatc cgagcccacg
    10501 agccagagct caaacagtca caaaaaaatt gggattatcg tttttcgcca gaattttctg
    10561 attttcagct ggtcttggat caattagcaa aaaaccataa cgaagtattg ttcatcatcc
    10621 caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg ttacaaggat
    10681 ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata gctgattttg
    10741 tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc tggaaaggct
    10801 ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt actgctagta
    10861 agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata ccagataaac
    10921 tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta tttttgaata
    10981 ttctgaataa aagataaaaa taccaagttt gcctgaacaa aaatcataag caatggtata
    11041 atcacaatat gaatatagaa atgggggaaa acatttggat acaaaagaag aacagctact
    11101 tattgattta acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt
    11161 cagagaatat gccaaaccat tcgctgatga tattttttat gacggtttag gcagtgtgat
    11221 tgccaaacat ggtgctggcg gaggccccaa ggtctttatt tctggacata tggacgaagt
    11281 tggttttatg gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa ctcttggagg
    11341 ttggtggggg caagtcatgc ttgctcagca agttgaaatc aaaacacgtg aagggaaaat
    11401 cgtccacggt gtgattggtt ccaaaccacc acatgtttta acacctcaag taagaaataa
    11461 accttatgaa ataaaggata tgttcattga tattggcgca tcaagccaag aagaagccaa
    11521 agagtggggc atacgtcctg gagatatggt cactccatat attgaataca aacgaatgaa
    11581 tggcagtaaa tatttactgg caaaagcatg ggataatcgt atcgggacag ctgtttctct
    11641 gcgagtatta gagaatctat ctaaagaagc acacccaaat gttttattcg caggaagcga
    11701 tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg taaatcccga
    11761 tattgctttt gctttagata ctggtacagc aggagacact ccaggaatga caccaaaaga
    11821 agcagattcg attttaggaa aaggcccaca gattttgatt tttgatgcat ctatgatccc
    11881 gcataaaaaa ttattgaatt ttgttattga tgtagcagag gaaatggata ttccattcca
    11941 atatacagtg atttctggcg gaggaactga tgcaggacaa atgcatttga caagagatgg
    12001 tgttccgtct gtagccatta ccgttcctgt tcgttatctg cattcccata catcagtgat
    12061 tcatgaagat gattatttga atacagtgaa actagtcaca gaattagtga aacggcttga
    12121 tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga ataatagaat acctatttct
    12181 gaatatcgga aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa tacttggctt
    12241 gttttcttat ctataatatt tacgagcgtc atccagaact tttttgacgg tttcagtcct
    12301 tttccggaag aaagcatgca gcagcactta ttgagttttc tagtcgataa tttactttta
    12361 ttcgtattca actatgcatt gttttacatt gcgctagtag ttgtacgggg tggcagacca
    12421 aaaagtaacc ttttattttc agttttccaa agaaagtatt ttggttcgat acttggcatc
    12481 aatttgatca attcagtcat caactggcta ctcaacagtt taatctttct gcccatgttt
    12541 ttaatcagcg gagtgagtac ttatatgcag ctgcttttca gttcaaatgc ggtacctacg
    12601 ggtgtcatgc cagaagattt tcttgatatc agctttgttt tatcgatgat agggatgatt
    12661 tttgtttcag cactcatcat gagtattgtt ggcggtttat tccaatttgc tgcatggaca
    12721 aagttcgact atcctgattt gtcggttatt caatgtttga aatatggctg gtttttattg
    12781 aaagatagaa ttggtacgta tattttgttg cagctgtctt ttatcggctg gtatttgcta
    12841 ggagcaatcg ctttgttcat cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt
    12901 gcagaatttt atgaacaagc acggattgaa aaaggaggtc cagacgccta ttttacttag
    12961 catgtaagca gtttattcat caaaaaaata acttttgtgg taggataaat ctttgagggg
    13021 gaatgtacga tgaaattaac tgttttagga tgcttaggag cgtatcctta taaaaatcaa
    13081 gggacaacag gttatttgct tcaatctgac gggttcaatt tgttgatcga tgcaggtagt
    13141 gctacgctga tcaaattaca agaacacctt gatccacttg atctagatgc tgtgatcctt
    13201 agccattatc atcatgatca tattgctgac cttggtgtat tacagtatta ctggcaactt
    13261 catcctaaga gaaatccgga gacattgctt tcaatttatg gacacacaaa agattccatt
    13321 cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa
    13381 gaattaaaaa ttggtccttt tctgattact tttatggaaa caatccatcc agtagtatgt
    13441 tacgccatgc gtatcgtaga agaaacaacg gggaaagtat tcgtatttac aggtgattca
    13501 ggttatttgg aaagtttcgc tgattttgcg aaagatgctg atttgtttct agcagatacc
    13561 tatctttttg aaggaaatga acatcatcat gcccatttca cttcaaaaga agctggagaa
    13621 cttgcgaaat cagctaacgt aaagaaactt gtacttacac acctacctca gtttggtgat
    13681 ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca aagggattga tatagaactt
    13741 gctgcagttg acaaagtgtt taacatttag gaggaagaac atgatttttg taccaaatga
    13801 aaaccacgac ccaagaatca atttagcaat tgaacaatat ttgctgcaag aaatgccttt
    13861 agatgaacct attttgctct tttatatcaa tgaaccttca atcatcatcg gcagaaacca
    13921 aaatacgctt gaagaaatca atcgagatta tgtggaagaa aatggcattc atgttgtccg
    13981 tcgcttaagt ggaggcggag ctgtctacca tgattttgga aacctgaact ttagttttat
    14041 catgcctgat gatggggatt cattccgtga ctttgcgaaa gtaacacagc caatcatcca
    14101 agcacttcat gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg atctagtcat
    14161 tgatggcatg aaattttctg gcaacgcgat gtatgctacc aacggtcgga tgtttgctca
    14221 tggaacaatt atgttcgact ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa
    14281 agacaaaatc gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga atatcaaacc
    14341 atttcttcca gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt
    14401 gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg tacgaattga ctgatgaaga
    14461 ttggaaaaaa atcaatcgaa tctctgagga atattatcga aattgggatt ggaattacgg
    14521 gaagtctcca gatttcaatt tctctcgcca aaaacgtttt tctatcggtt cgattgaagt
    14581 ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa atctttggcg atttcttcgg
    14641 actaggagat atctctgacg tggaaaaaaa attggttgga cagaaatatg ataaagcatc
    14701 tctatcaaaa atagtcagcc aaattgatgt gaagaaatat tttggtgcga tagaaccaga
    14761 agaattgctt accttacttt attgaaaata aaaaatagcg gaatccacat atatggattc
    14821 cgctattttt tatacaggat tcgggtaatt tcgagagagt tgaacagtga tccgatcaat
    14881 gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat
    14941 cattaagtca aaacgttcct gatcatctaa ttcttctgtg atgcctcgac taaataggtt
    15001 tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat
    15061 cgacaaataa atttttcgat acagtggatt ttcgatggca tgagtttctc taaaggaatg
    15121 ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg ttgataggaa gttcttcggg
    15181 aagatctgct tggaaggtta tctgaatatt ccgtttggca gctaacttct ggtaacgcgc
    15241 taagataaga tttgtcacct ggtggttgca aaaagaacac agcatttcga aattttttgc
    15301 ttcttctaat atcttttcca catagttttt agcttttgtt tggtccgtat gcattaaatc
    15361 ttgcagattc tccatctctt ggacaaattc caggcgaagt gtatttactt cttggatatg
    15421 ctgtcgaagc tgctcataat gttgctccaa caaatcattt ttgattttta agaaatctac
    15481 ttgtctccga tctttgtcgg tatttaagat gtgatcgatt agattgtagc tgataataac
    15541 gattagaatg ataatatata aaatcgtaga ccaattgatc gttgtttgat agatacctaa
    15601 tgccgtttgg atgtaaatca agcaatggat aatggctacc aagacgatcc aaggtgtaca
    15661 agctacataa aaacggttat tcttatatgc ttcgccaatg catgcaagtg aagtgactaa
    15721 agtgcttata acactagcaa tattagcaat cggcattaat tcaggaagtt ctgcaggagt
    15781 aaaagttgca taaagcagaa cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc
    15841 gtaccatttt ttatagtaaa acattcggct ccaatagtag cagatgatga aagctgacgt
    15901 taagataata aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa gaataccact
    15961 taaaatatct tctgatacag cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat
    16021 aagtgagtat tcaagacgtt tttgtttata catcaagtag atcgtataga aaaaggtccc
    16081 tatagcgata aaaacagcga ttatcatcgt caatacttgc ggcactgatt gtgaataaat
    16141 aaaactaatc aatggtccag ttgttcctag ataaactttc ggtggcagtc cagcatagtt
    16201 ctgataagga gatgagactg taagggtcaa tttttttccg atataatttg ctggtaaatc
    16261 tatcacagcc aatgagagtc cgggattgct ttcttcctga taatctttcc gttggtataa
    16321 aatcttttcg cctagatata cagtaatcca ttgatggttt cctttgatta gtaaatttgc
    16381 ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt tatccacggt
    16441 aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa
    16501 aacccacgaa tcttttattt ctttcatttg attgctgtcc gcaggagaaa aaataaaata
    16561 aaagaaataa gcaattcctg ttaaaaataa caatgataat ataatgatta tgctccactt
    16621 tatacgtaaa taaaaagatt cttttttatt atatatgtta ttaaaaaaag acataaaggt
    16681 attcctttct aaaacttgta atgttagggt aaacgttttt taaactaacg tcaattattt
    16741 ataataaaaa atgagataaa gatgaggaaa tagaattaga aacatataat tattaaaggg
    16801 aaacattttt acagctataa tttgaaaaaa atttatcgtt attatatgat taagaaggat
    16861 tgaataaaga aagaagttga aaacgatacc tgattacaaa aaaataactt ttataagttc
    16921 tataacgttg attatatttt taattcttat tcttctcgtc gtcttggttc cccgaatttt
    16981 tggtttgact ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa
    17041 tctgatatat gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata
    17101 ttatgaaaat tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca
    17161 cgaagatttt tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa
    17221 gagaaatatt atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa
    17281 aacggcattt gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat
    17341 cacagcatgg aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt
    17401 gtaagaatgt taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa
    17461 cacgagaaaa cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga
    17521 ggaggagtag tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg
    17581 attttatgcg tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag
    17641 ctcatgtagc agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac
    17701 ttggacagct tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa
    17761 gtgtcatggc cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg
    17821 tcatgatcga gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt
    17881 tgcttgaagg tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt
    17941 ttatggatag tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa
    18001 gaaaaggtgg cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag
    18061 atgaagagat tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg
    18121 ttgtgatgga tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct
    18181 taatgacaaa ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat
    18241 aatcgattga atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc
    18301 aattaaagga tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg
    18361 ttttcaattg ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt
    18421 agtagttggg ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga
    18481 agcaatttaa ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct
    18541 ggatagagca tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta
    18601 aaaaaccgtt agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt
    18661 ttgttttcaa aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca
    18721 aaaaaataaa actcatcaga aaagaggaag aagatatgac gatactatct agaattttag
    18781 tgactctagt tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt
    18841 ctgacacgac aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc
    18901 aaacattgtt taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg
    18961 gagcttatct ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt
    19021 ttgttgcatt atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc
    19081 tagctctgtt ggcattgctt tctattttct tataaatata gaaaaaataa gaatttatct
    19141 aacaggaaac tttgtgaaaa atattacttt atctcatgaa atatgatatt ctaatgaatg
    19201 gtattttaga aaatttcatg aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc
    19261 actaaaaaaa gaattttcag gttatgatct ttcaagattt cagaaagatc ttttggctgg
    19321 gatcacagta gctgctgtag cattgccttt agccttggct tttggggtat caagcggagc
    19381 tgatgcggcc gcaggtttga ttacggctat cattgccggt atcgttattg gcggtttatc
    19441 aggcggtttt tatcaaatat caggtccgac aggtgcgatg gcagctatct tgatgtctat
    19501 tgcggcaaca aagggaatgc aaggtgtatt gcttgctact tttttagcgg gtctattttt
    19561 acttgcagca ggacttttcc gattaggaac actgacttct tttatcccag cacccgtaat
    19621 tacggggttt acttcaggta ttgctatcat tattgcatta ggtcagatcg ataatttatt
    19681 tggtgttcac tcggaaggta caaacgttat tgaaaaacta agtagttatc aaacgatagg
    19741 atttccgata aacacagcct ctttgatcat gggagggcta gtcattttag ggatgtttat
    19801 ctatccaaaa aaatgggcga aacgaatgcc tagttcgctg ctggctatca ttttggcaac
    19861 agcagttatg ctattgttcc atttaccagt agcaactgtg ggtaagattc cacaaacatt
    19921 gatcagtagt aatcgtttga atatgggaga ttttagcttt tctgccttgc aacaagtggc
    19981 agttccagct attagcattg ctttattagg aatgattgaa agcttgctat gtggtgcttc
    20041 ggctggccga atggcaaata agcccttaga tagcaatcaa gaattaatgg cgcaaggaat
    20101 tggaaatctg ttgcttcctt tctttggcgg gattcctgct actgcggcca tcgcccgtac
    20161 aagcgtggcc atcaaatctg gtgcacaaac tagattagct ggtatgatac atgcagttgt
    20221 cttactgctc tccatgcttg tttttgcgcc agtgatgtca cagattccaa tgccagcact
    20281 tgcaggtgtt ttgattgtga cggcttggcg tatgaatgaa tgggaaacga tcaaggagct
    20341 atttactaat aaatattggt cagcagttct attgtttatc cttacgatga tttgtacagt
    20401 tatctttgat ttaagtattg ctatcgtgat cggggtgatc ggcggctgtg tattttttgt
    20461 tgtcaaaagc gctgctatca cgatttcatt agaaacgatc gattggcaaa gaatggggct
    20521 gccagaaacg gaaaagctga ataactggac agtagtgtat attagtggtc cactgttttt
    20581 tatgtcttca gaaaaactca aaaataaatt ggaagaacta aatcatcacg atggtgtcat
    20641 tttctccatg cgaggcgtac caagtattga tttgacggca cgtgcagttt ttgatgaatt
    20701 tcaagaaaaa gcagagcaaa gaggacaaac gattatctat acttccttgc agccagaagt
    20761 agaaaaacaa ctttctcact tgtgggaaca tacattcacg catcaacatc aaacagttgc
    20821 tcatgcgttg acagctttac atgcgaaata tactgcaatt agccattaaa acaatgaaaa
    20881 agaacagaca agactgtaaa ctgaagattt tatggtcttg tctgttcttt ttaatattgc
    20941 tgtaaaagtg aagctagtac ttgccagtct tgatggataa caggtacaag ggagcgattg
    21001 tagaaaatag ctgctggatg aaaaagtgga agaattcgat agtttttttc tgaccattcg
    21061 taggcatcct tttgttctgt taattgtaag acaggccctg tatagagttg accatgattt
    21121 ttggtaaccg tatattcttt gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca
    21181 attaattttg gctggacctt catcagttcg taatccaata ttggtgcatg cgcaaatact
    21241 tcggaacgag taggtgtccg atttggataa acagtttctg actgttgtgt ccgtttattg
    21301 atccgatgag tcacgcgata cgggcggctc cttacagcac tggtgatata cacctcttct
    21361 cgtgttaagc cagttgaaga aagtgcctgc atcaattctt ttccagcttg accgctaaaa
    21421 ggaacatgcg tatcaatctc tgttttccct ggggcttctc ccacgagcat cagtttagga
    21481 tgaagaggtc cttgcccagc gataaatcct tctacaggac ggccgttcat tcttaaagaa
    21541 gcttcttgta tcagttcttt tgggtattcc atttatgctc ctcctttttc ttttattata
    21601 agaataaaag aaacacaatg cgactatttt atttggtaga actgtgagga tagaaaagca
    21661 gaagtattgc ttgaattttt gttttacttt ttgattttaa atttagttga ctaacggttg
    21721 ctgttctggt aagctagtac tgttgtaatt gtggactcca cagctacaac cgctcagaac
    21781 aagtaataag cacagcatat gctgtcactt cggatggcga gtctaagtat atcagaagga
    21841 ggtgctttat agcatgtacg caattattaa aactggtggt aaacaagtaa aagttgaagt
    21901 gggacaagca atctatgttg aaaaacttga cgttgaagca ggagaaaaag ttgtttttga
    21961 cgaagttatc ttagtaggcg gagaatctac aaaagtaggc gctccaactg ttgcaggtgc
    22021 aactgttgaa ggtactgttg aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata
    22081 caaacctaaa aaacattcac accgtaaaca aggtcatcgt caaccatata caaaagtggt
    22141 tatcgacgcg atcaacgctt aatagcgttt gagaaagaag gcctatatat gatcaaagga
    22201 acatttaagc gaaatgacgc aggtcagatc gtttcattca ctttgaccgg gcatgctgat
    22261 gctggtcctt atggaagtga tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact
    22321 gtcaacggga ttgcctcatt agccggtttt gagccgattg ttgaaatgaa tgaagaagaa
    22381 ggcggttatc tttataccga agtcacttca ggaatgacac aagaacaaaa caatattgct
    22441 gagattctat tagaaaatct tttattagga ttacaatcga tagaagctga aaactcagaa
    22501 tatatccaaa ttaaaacaat taatgataaa taggaggtgc agaccatgtt gttatctatg
    22561 aatctacaat tatttgccca caaaaaaggt ggcggttcta catctaacgg ccgtgactca
    22621 gaatcaaaac gtctaggtgc taaacgtgct gatggacaaa cagttacagg tggatcaatt
    22681 ctttaccgtc aacgcggtac aaaaatctat ccaggtgtaa acgtgggtat cggtggagac
    22741 gacactttat ttgcaaaagt tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa
    22801 aaacaagtgt ctgtttatcc agtagctcaa gaagcttaat aaataaactg ctctatctct
    22861 tgtgtagcaa gggatggagc ttttttgtaa attatgggat tttttttgac catacttttg
    22921 accatacttt cttagaagtt aatataattt gttagttttt gtgttgtttc ttcttttttg
    22981 ctgttagtaa tgtgtgtata tgtgtccata gtagttttta tatttgcgtg gcccattctt
    23041 tcttggactt ctttgtgatc tgcgcctgcc tcatatagca ttgatgcatg tgtatgtcta
    23101 aaaccatgca gaccgatata aggtaagccg gcacgctcac gatatatatt gtatctttct
    23161 gccactgact gattaattgt gaaattattt tcgtcattgg taaacaaata attagcttta
    23221 gtaaatccta attgaaataa gtactttctt tgttcgagct tccaaaattt tagttgcttt
    23281 atagtttttt catctaaata aattattcga tttgattttt tggttttagg agtttcagat
    23341 atataatatt tatctgaacg agctacagtt tttttaatgg ccatttcatt tgttttaaaa
    23401 ttaatattgt cccaagtgag tgctaatatt tcgccgattc tacatccgct gaatgctaat
    23461 agtctaaaga gcgtatagtc tctgttttta aaatatggat tctgttcttg agatacttca
    23521 tttagaaata attccaactg ttcttttgta tataatttta atttcttttc tgttttaatt
    23581 tgtggctttg gaataagtaa atttaatgtt ggattatcag atgttaaacc aatatttata
    23641 gcatacttaa aaactttatt catatagttt atgaaaagag ggtactgctt tgaagttcct
    23701 ttttcactcc aatcatttac taccttttga caatatgccg tattaatttt agaaagctta
    23761 atatttccaa atttgggata tatatgttta ttaaagatta ttttagtcct tgaatatgaa
    23821 ctttctttta cagttttttt gtagctttct agccataatt ctgctgcttc tttgaaagta
    23881 gtatttgtac ttgtgggcat tcctgttttt tgtaattcta gttctaacct agaaagtgct
    23941 gtttttgctt ctttttgtgt tctaaatcct cgtttagtag tatatttttt ctttccagtt
    24001 agcggatcaa taccgagata agttttaaag taccatgctt tttcgccatt tttcttttta
    24061 tattgtttta tcattgccat caatattacc cctattcaaa ttctaattct gccatgctat
    24121 cattgaaaaa ttcgatcagt ccttgttcct ctataaattc aagccatatt tccttcccgt
    24181 taattaatgg aactcctttt tctttacgaa ttgtttgata caagttagtc acgacttgtt
    24241 tccacatttc taatacagct ttatctattt tcttatcatc ccagcgtttt ttttggggtc
    24301 tatttttgtt tttttcaatt tgataattag tagctttgat gataaatgtt tcgtatccgg
    24361 ttgtgctttt ttctaaccat tctttgaatt ctgtatatat catttttgtt cacctttcta
    24421 tttttgaacg tatgttcttt tttgcttaaa aaattttaga gagctgcata acaatagatg
    24481 cgagctttta ataattcttt cacaagtggt tcgaagcgaa cgcttaaatt gttatcttct
    24541 ataaatttca tgtaattgat attgtgtggt tctaattcag actttgcaat atatctatct
    24601 aataattttt caatcatgaa gcgatcagcc tcatattcca tttttgaatg gagagagaat
    24661 gctaagttat ataaaaaata attatcatga tgtttagaag cgtgtcctaa ttcatgtaat
    24721 aaggttcttt gtcttctata cttagataaa ttagctttta ttactatggt attcatacaa
    24781 gcaacataat ggccatcggc atcaaggttt tcacgctctt ctaccttaac gccgagttca
    24841 ttaattatca tttcaatttg actgtccaaa tgactcacct acttatttag ttatcttttt
    24901 tctctaaata tgcttcaatc atagctgata gtatttccct atcattatcg gtgagcggtt
    24961 ttccgtcgct actcataaca gatgctaaag cttcttccac ggttagtttt ctttcctctg
    25021 gtgcaactcc cgcgttagga tcatctgtgc gtcctagcaa ataatctgtt gatacatgga
    25081 agtagtcagc aactttttgc aaagtttcta ttttaggact ttgtgtcttc catttataaa
    25141 tagagttttt tccaaatcct attctatttt ctaattcaga aatagaaata cctctttttt
    25201 tacagagctc tttaaccctt tcgaaaagca tgtcaaatca accttcctag agcgtacgat
    25261 aataatttat acaaaaagat agaaaggtag ttgacaaatc tatccaattg tcttatattt
    25321 aactcgtaag ctaatttaat aagctaacaa gcaacaagaa atacacctaa taaaataaac
    25381 gaattcgagg tcggcaaact aagaatgtta atttttaggg tttttctagg cttatttaac
    25441 tgtatataaa tataagacag ttggatagaa atgtcaataa aaattagcta attttattag
    25501 cttacatatt taaagaaaag gaggaaacat catgaaacaa aataaaaaaa ctccttccgc
    25561 aaaaggagct ttaataactt taaattttaa agaccaaaag tttagtgctg ttgttgatga
    25621 aattcatcca caagagcatc aaaaaaattc ttttgcaact gacgagaaaa agactcagaa
    25681 acgctctgag aaagttcttc gaataattca atttctgttg gaaaagattc gtcattttgg
    25741 aagtctattt tgaattcttt attaacagtc ttgaatacgg tttgtttgaa taaggcgtcg
    25801 acatccatat taaattcttc catactattt tcacctcgct ttctattcaa aattatatca
    25861 aagaaaggaa tgagcaaaaa tgaacacacc gcaaattttt aactttgaac aacatgaagt
    25921 aagaacagta acaatacatg atgaaccatt ttttgttgga aaagatgtgg ctaaagttct
    25981 agggtaccaa aatggtagta gagatattaa cagacacgtc gatgtagaag atcggcaaaa
    26041 ctaccaaaac ggtacttttg aatcgcctag agggttaact attattaacg aatcaggttt
    26101 gtactcctta attttaggaa gcaaacaacc aaatgctaag aaatttaaac gttgggtgac
    26161 tagcgaagtg ttgccagcaa ttagaaaaca tggaggttat ctaactccag aaaaagtaga
    26221 agaagctttg cttaatccag atacaatcat ccaattagca actaagttga aagaagaacg
    26281 tactggaaga ctaatcgcag aacaaaaaat tgcagaatac gaacccaaaa tttcgtattt
    26341 ggatagtatc ttatcttcta cagattcagt aactattagt caaatcgcgg ctgattatgg
    26401 gatgtctcca caacagatga acaaactact tcataagcta ggtattcaga aaaaagtagg
    26461 caaccaatgg ttattatgca aaaaacacat gagacagggt tacacaaaat ctcatacaac
    26521 tgagattccg aaatctgatg gcggaacaaa agttgtgatg aatacaaaat ggactcaaaa
    26581 agggcgtctg ttcatttatg agtcgctgaa aaaagaagga tatattccag aaatcgattt
    26641 attagaggag gaatgacgat gtcacaagaa tttattttga aagtcagaat tcaattagca
    26701 aaatacggaa agtctcagaa ctggttagct gatactatcg gaatttctag accatatatg
    26761 tcggacatta tgaatgggcg tagaaaacca gataaacaaa tcaagccaat tgaggcagca
    26821 ctagcagagt tagagaagga gaaataacat gcaaataaca attccagata atttagtagt
    26881 ttccgaatta actacacaga ttacgaatgc tgttcttaat tcattggacg aacgattaca
    26941 ccttatgaac aaatcagtag agcttcctcc atatccaaac aaatcagagg taaaaaaagt
    27001 tttaggcatt ggtgatgaca aattaacaca ttggataagc ctaggcttaa aaacacagca
    27061 gtggagcaag ttagacatca gaattgaacg atcggaactc caaagatttt tgaaagaaaa
    27121 ctttgagttc taaaggcaaa ggagaatgat tttatgtcct acacattgca acaagaacat
    27181 caaattctag gtctgattaa acagcgtaga aaacaattac aagatgatcg tgcagcgctt
    27241 agaaaatccg atgagctatc agatagacaa gctgaactaa ttgcttctga acttgaggat
    27301 ttgagaatgc tagaaataaa aaatagggag attagattat gaagaagaca gacacacttt
    27361 ttataggatt cattttgggg ttattagtga ttgtagcgca ccaaagtatt atcggaggaa
    27421 gcttgttcgc agcattgatg gttttaatca atctgcttga ctcaaaagaa aggagcaact
    27481 atggcacgag aagaagcgct aaaaatcggt aaagtgattg ctgataattg gtgggcaaat
    27541 agccgttcta ttattttaag caagcaacat atcgaaaaga aaaaagcatg gcaacaaata
    27601 aaaaagtgac tccgccgacc aaagcaatga gtcactcaaa aaatcataac taaggagatt
    27661 ttaacatatg gaaaatgaac tttccactct agatcaatat ttggccgatc ctgattgggg
    27721 caaatcgaat ttacaactgg ttgctaacaa gtacgaaaaa tcaattgatg aagcaattgt
    27781 tgcattgact gatgcagctt ccataaccgc tgatgacagt aaatggacca agagagactt
    27841 gggcattcta aaacgaggcg ttaattggct tgaagatcag tacagagaag aaacaaaaga
    27901 gaagtgatat gagtgtttaa accactaata gattcatact cggcggtact aaagaaattt
    27961 aaaggtaacg acattggtgc aacaatcaac gaagaagtaa atatcgaacg gctgaaaacg
    28021 atgtatgacg gatatgatgg cgatcgagtc attgaaattc gctttattga tccacgacgg
    28081 ttcacagtac agcaaagaaa cttcatctat gcgctcatag gcgatatttt catcgataca
    28141 ggcatgccaa cggacttctg gaaggaattc ttctacttcc gttttgaagg tgtcacaggg
    28201 cgcgaaataa gcctcaaaga cgaatcgaat acaaccgtga gtgatgccaa tatcttagcg
    28261 aatatcatcc tagatttcat ctttgaacat catattcctt tcaaagaagg atatgagatt
    28321 ttaccagcga atcaagagta ttacttctac aaatgcatta caaaaagagt ctgctgcatc
    28381 tgtggcaaaa caggagctga tatcgatcac tttgacaaag cgctgggaag acgaaagcgc
    28441 aaagaagttg atcattcaga gtacacattt gcagcactct gcagaatcca tcacacagag
    28501 aaacacaaaa taggtgtgat caatttcaaa aataaatatc aaatcaaagg gatcaagtta
    28561 aaccaggaaa caattaaaaa gttaaggata ggaggataaa tttggctgag ataagttgga
    28621 tcaaacttaa aactactatg tttgacgatg aaaaaatacg attaatccaa gctgttcctg
    28681 agtcggatgc catcatcgtt atatggattc gattactagt tttagcagga aagactaacg
    28741 acgatggtct gatatatatc cagaggaaca tgccttatac cgaagaaatg cttgctacat
    28801 tgtttggcaa aaacgtaaat acggttcgct tagcgctaac tacattggca aatttcaaca
    28861 tgattgatct aagcagtgat ggactaattg ccatcagtaa ttgggaaaaa catcaaaata
    28921 tcgagggtat ggataaagta aggctaaaaa atgctgaaag aaaccgtaaa tacagggaaa
    28981 gaaagagaca ggaacgtctc aaattggaaa atgacgttag cgtgacgtca cgtgacggta
    29041 cagataaaga tatagaagaa gataaagata tagataaaga agaaaagaaa ggtaagtatt
    29101 ctgacgaaca cttacgcctt gctaaaaagt tgcaaagtaa tttaactgaa gattttccaa
    29161 aagaaatgaa caaagtagat atcgaaaaat gggcagacac aatcaggttg atggaagaaa
    29221 gagataaagc atctatagaa gcgattgagt atgtgatcaa ttggctacct acaaatgaat
    29281 tttggtttgg aaatattaga agtgctaaga aattgagaga aaaatttgag aagctcaaat
    29341 tcgaaatcaa agcagacaag aagaatcata aaaagcaaag tcaaaaacta cagtacagca
    29401 atcctagtga atatgacgac ttgccaattt aaaaaggaga tgcatcacat ggaaagccta
    29461 gcaaatgcta tggagaaact aataagaaga gtattagtgc aaagcggaaa atgtccagaa
    29521 tgtagcgaac ctttgtatag ttggcgagct aaaaataagg atggttcaga acgttgtaaa
    29581 ccaacatgca tgagttgtgg ttataaagcg ttacgtgtga aagaggatat acagaccgaa
    29641 cggatatata acgatagctt aaaagcacga gcgttgagtt tttttcaaaa tggttcggta
    29701 ttaacagata aaactttgtt taaatgcaaa atggaaaatt atcacgtagt ggatcaagaa
    29761 acaaaaatcg ctttagaaaa agcaaaaagc tatacgaatg aggttctgct gaaccatcct
    29821 gcacatttca ttctatcagg gaaatcagga agcggaaaaa gccacttatc aatggccaca
    29881 gcttgggaaa tacttgagcg ctcaaattat gacaagaaaa tactttttat aagctatcaa
    29941 gaattattag agcaaataaa gttttcttat aacaatactg aactgagaaa agaaattgaa
    30001 ggatcgctta tagccgatat taaaacaact gatttggtgg tttttgacga tattggagct
    30061 gaattaggta gtggggtatc aaatagtagg cagtttacaa acaacacgtt aaacacgctc
    30121 ttagaagcca gacagaacaa ggcaacgatc atcacaacaa acttatctgg tcctgaacta
    30181 agagaagcct acggtgaaag aattgtttct aggatattta agaattcaga aggttatgcg
    30241 ctgaaattcc aacaaacagc agacaagcgc ataaaaccag tgaaaggtag tatcgcatga
    30301 ataaataccg taataaaaaa actgttcatc gaggtatcaa gtttgattct atcgcagaag
    30361 cagagtatta cgatctagcc ttgtggcaag ctgaagcaaa tggctggaaa gtaaaacttc
    30421 aggaaagatt tgagctgatg ccgaaatttg aactagacgg aaagaagtat cgcaagatcg
    30481 agtatattcc cgacttcaca ttttataaaa acggcaaact tgtcaaagtc gtagatgtca
    30541 aaggaatgca gacaaaagac tttaagatca aggcaaagtt gttctgtcat caatatcaag
    30601 cgccgttgat attagctaaa aaatatcgga atacgttcaa ggaagagcgt ttttaacgag
    30661 gtggtccatc atgacaacag aagaagtgat tcaaatgcgt attcgaaaca ttcagcgtga
    30721 aattgacgat ctagaacgaa caaaggcagt gatggtcaat gaaacggcga gaaaggcaat
    30781 cgatttgcac atagagaact taagaaggga aattcgtaga ttggaggaat gagcgtggat
    30841 aagaaagcga caatgaaacg aattgctgaa ttaaccaagt cagaatcttg gcaggaagac
    30901 aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga aaagcctaaa
    30961 cggaaaacgc cgagaaaaat tgcaatctgg catggtgatc gaattctagt aacaggtaca
    31021 gctgaacagt tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctagg
    31081 agcttatgga ttgattcaaa aggacgacag tttaggtatg tggaggagag ataatggatc
    31141 tcattacaca atacagtgac atcatcctca agaaaatcat gatgaagatt cagaaagaca
    31201 aaaaatcaaa agaacgagct gaattagtta agttagaaat ggctgaaaca ggagcaggag
    31261 tgcgaagtag caggcattgg aaagcagcag caaacattga attttattac aacgaaattc
    31321 aaaaagggtt cgatcagatg cgtgagctgg atcggcaaac aaattggagc aagaaacttc
    31381 atcaagatcg tttcaaattt gtagagaagt ataaagaaat attagaagag tatttgagga
    31441 ggacagcaaa tgataaaaaa actcgttcaa ttcagcatgg atttatatga tatcgaatca
    31501 ggagcaacac tatctgtgga atcggaccat ctaatcataa attttggtgg aaagcgccag
    31561 attattttgt gggtagttga tgatgtactg tttccagaaa ttgttcatga tttcgaagaa
    31621 tcaaaagcgg ttgagtttga aatagtgaaa aaagtaatgg aattgattga aaaatacgag
    31681 gaggacagcg aatgataccg aagtttagag cgtgggatag agaaaaagac agaatgaagg
    31741 cagtagcttt ggttgaattt gctgaggcgt ataaatcagg agaactcatt tcacgtacaa
    31801 aataaaaaaa gtcggattcc tccgactatg aataatattt ccgacataag tattatatca
    31861 taattggggg aatcagagga tggtactttt cgatgtaaag aaatatgaga caccaagcgc
    31921 gaaagatgtt gatatggaac gcacaaaaca taatgtcgct gtgtttcttt cagcatatct
    31981 atcagctaga tgtagagtag ggcaacctcg agaaccaaaa gtgacagcat cttattcctt
    32041 ggttccacct tctacagctg atcatatatt tgaagcagaa agaatgctga tcgataaaga
    32101 agaagcacag gaagaatttg agtatctgca taaattgttt gttcgaggct attcagcgat
    32161 acagcatccg cataagcctg atgtgactga aaggcgcaag aagatattct atgatcgtta
    32221 tatcaatggt ctgcccattt atgtaactgc tcaaaggaat aatactagcg aagaatcggt
    32281 taaagtagaa tcaaacagaa ttatcatcca atttgcttca tcgttagaac tggttgcttt
    32341 caagtagcca gtttttacac tttttatacc cttttattac caatttggtt tccattttat
    32401 acctttttta taccaatcac ttacctattc aacgttgtat tatgatagtg tcgaaagatt
    32461 aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
    32521 gcttcgatag gcagcagcgg aaatattaag aataaggatg tgaatttcaa ctccttctaa
    32581 attgttctta ttatctatca tccgttgctg tctattaatt tatgtattgg agggtatata
    32641 aaatgaagat cgcttccgaa aaaattgatc aactaacaat tttagtaaac caaagaatcg
    32701 aagatattat caaagaattc gaaggtgaaa agattaagaa cataaagctt actttaatgg
    32761 atcataagtt cgaattaaat tcgacaaaaa ggaagatcgt tgagcttata gaagaagaaa
    32821 tagctctcca gatcaatgag caacaattag ctaattaatt tatgtattgg aggggaaatg
    32881 aatggaagtc gaacaaataa agtatcaagg tatggatcgg gaacggttga ttgatattat
    32941 tgagcaacaa cgtttagaat tgatccaaaa agaagctgta tgtaaagagt acaaaaaaca
    33001 tttggaacaa gtaattgagt atcattcagt agaaaagtac agatcagtgg tgcaaaaaaa
    33061 tagagcaacg agtcctgatt ctcgtcaaga tgtatcgatt agtatcaaga cgccagtatt
    33121 taattctgtt aaatcagaaa agctagaaag tatctcaact gctttggtag aaaaagcagc
    33181 tgaaatgaac tccctagcaa atgaagttag tcgtcttatt tagtcatatc gataacttga
    33241 gagtctttgg tgattccgtt ttttatagaa ttaatggttt gttcagcgga atatttagtc
    33301 aagtatgttt cgctagttgc tactacttca ttgttattgg acttaataac gaagtaatat
    33361 tggccattag ttgcttctct tataacaaag tacaaatttt tcaccaccta taatttattt
    33421 cagcggacca ctcgctgata attaaaatta taagctatgt ataaaatttc acaatatcaa
    33481 tttgtcgctg tggcggaaag ggtagacgca ataaaggatg aatgggaaaa gtcaatgagt
    33541 aattatctga aatttacaag tcccctgttt agttatctaa gattatttga taatattatt
    33601 tctgaggtga tagaaaatgg tgggcgtagt taaatttctt gggtttacat ttgatggata
    33661 tggtacgtta ggcgaatggg taggtattat tttgatagtg attacaattc aacaaacatc
    33721 aaagtactac tggtattcta atagttctaa aattgtatca agaataactg aaggaataac
    33781 caaaaaagat attactgtcg tcggtaaata tgggtttact ataactttat taaatgaagg
    33841 gaaatctgaa accgcaataa agttttgcgg gattataggt aaatataaca ttttccaacg
    33901 ttttatcaga aagcattggt acaaatatat ttacaaatac cctgtagtta aaaagcttat
    33961 tgaaaaatat gtggaccacg aaatatctta tctcaatcca acagacctta ttaatcaaga
    34021 attccagaat attggatact tagaaaaagg tctaaaaata ccaattgtaa atgaaagttt
    34081 agttactgca atggtagcta acataaagaa cagcaagaaa ttagaaaaga gatataaaaa
    34141 atgtaaaaaa ttgaaatttt cctttttatt cttagaatat aatggcaaag aatattatca
    34201 agatatttat gttttgcgag gctcgaactt atataaagac attaaaaata agatttcagt
    34261 atgagatttt ttaaaattcg tagatcactc tttgagtggt ctttttattt tgcgtaaagg
    34321 aggcacgacg atgtatagac cacaatactt agaacagaag catgaagtaa tcactgtgca
    34381 aaacggtaac ggtgagatag tacgaaagta tagaagacca ataaagagcg atacatataa
    34441 acggaaggaa agcaatgaag ttattccatt gtatggcaaa agaatagcta agtattaaat
    34501 aagattgcga aaggagatgg gacatgaccg aggaattcta tagatggtta ttacagttga
    34561 taagagaaga tcgtttggtt aagttctatc agtctcctaa atggcgcagg cttagagaga
    34621 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
    34681 gagtagagaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
    34741 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
    34801 cagcgttaga taaacaagag aagaagatag aaagcttcgc taacttcgat gcaagtgaaa
    34861 ggtggtaagt gcatgatcat caatgacaat ggcagagagt atgatacaga aaagattgaa
    34921 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtaggt
    34981 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
    35041 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
    35101 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
    35161 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
    35221 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
    35281 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
    35341 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
    35401 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
    35461 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
    35521 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
    35581 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
    35641 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
    35701 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
    35761 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
    35821 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
    35881 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
    35941 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
    36001 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
    36061 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
    36121 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
    36181 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
    36241 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
    36301 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
    36361 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
    36421 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
    36481 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
    36541 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
    36601 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
    36661 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
    36721 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
    36781 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
    36841 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
    36901 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
    36961 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
    37021 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
    37081 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
    37141 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
    37201 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
    37261 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag
    37321 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
    37381 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
    37441 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
    37501 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
    37561 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
    37621 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
    37681 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
    37741 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
    37801 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
    37861 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
    37921 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
    37981 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
    38041 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
    38101 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
    38161 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
    38221 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
    38281 atggagtatt tacaatcaac gaaggaagag agcgcgttgg aaatgatcgc tctgataagg
    38341 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
    38401 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
    38461 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
    38521 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
    38581 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
    38641 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
    38701 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    38761 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    38821 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    38881 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    38941 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    39001 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    39061 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
    39121 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    39181 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    39241 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    39301 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    39361 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    39421 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    39481 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
    39541 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    39601 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    39661 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    39721 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    39781 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    39841 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    39901 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    39961 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    40021 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    40081 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    40141 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    40201 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    40261 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    40321 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    40381 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    40441 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    40501 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    40561 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    40621 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    40681 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    40741 caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa ttattggaaa aaggtttatc
    40801 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata caaatctgga
    40861 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    40921 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    40981 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    41041 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    41101 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    41161 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    41221 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    41281 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    41341 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    41401 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    41461 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    41521 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    41581 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    41641 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    41701 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    41761 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    41821 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    41881 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    41941 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    42001 gaaaatagag aagatttaac aggagattca ctagatatta tttcattgat tcaaaatcgt
    42061 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    42121 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    42181 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    42241 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    42301 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    42361 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    42421 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    42481 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    42541 tctgattcgt caattcgata aattaaataa ataggaggaa caacaatggc taaaacaaca
    42601 acagtaacaa cttttgacaa tataagtatt aaaagaattt cttttaattt taagaatgca
    42661 acaaatgcaa tttcaactga ttgtaatgga caattagatg gcgaaacaga aatgcagaga
    42721 attgtaaaaa aatgcggttc aacagaagta aaatcgaaat ctaaaccaat caatatgacg
    42781 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gcttcaatgg gttgaaacaa
    42841 gatgaacgta ttaaatcggg aatttactct tatggtcctg attccgtagg ggaagatttc
    42901 tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa ataagctgat tggtatgtta
    42961 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
    43021 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
    43081 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
    43141 gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg
    43201 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
    43261 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
    43321 cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc
    43381 aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt cattcgatga
    43441 atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat
    43501 gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa
    43561 gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
    43621 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
    43681 attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac
    43741 caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga
    43801 agtaacattc atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga
    43861 attaactaaa aatagagcag aattaaaact agaacaagct caattacaac agactggttc
    43921 tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag
    43981 tcaaaaagtt gaagtaacta gccaacgttt agccaatgcc aaaaaatatt atggagaaaa
    44041 ttccaccgaa gttcagaaac ttgagagaga actgattaac caacaaacag cgcaacaacg
    44101 tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca
    44161 gacgtacgag tctacaatgc aacagttgga tagtgaacaa aaaaatgttc aagctagtgc
    44221 ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga
    44281 atccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcagagaa
    44341 aacgatagat atcctgagac gacagttaga agctacacag tctgagtttg gcgctacatc
    44401 cacagaagca atgcagatgg aggcgaagct taatgatgct gaacgtgaat ttgaagagtt
    44461 aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga
    44521 tatgaataat ctaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga
    44581 attagggaaa caagcagtgg actctgctaa tagtgtaggt agttcccaga gtaaaataca
    44641 agctaatttt ggtttgacta aacaagaggc tgaagaatta acgaatgtag ccagagacat
    44701 ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa
    44761 gcgtaattta ggcgatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt
    44821 cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat
    44881 ggtcaatttc ggcttgagcg ctcaagatgc aatggattta atggtttcgg gtactcaaaa
    44941 cggtttagat aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag
    45001 tcaaatggga tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg
    45061 tgcttataac cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga
    45121 tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga
    45181 gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag
    45241 caatatggat ggtcaatacg accaattaaa taaagcttct acaatttggt ctgcacttgg
    45301 cgaagataac gcgatgaaag ttgtccaatc tttgactgat gttaaccata catttgatga
    45361 tgttagtgga tctgcacaaa aaatgaatga agattctact actccgttac aagaattaaa
    45421 cggaaaaata gctgaattaa aggattcatt agctcctata ggcaacacaa tcatagatgc
    45481 actcgaacca gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca
    45541 accagtacag gattatgccg tagcgattgg cggattgact gctgcattta ctttattaat
    45601 gccaataata gttggcttca tggctctagg tggtcctact acattaataa taggagcagt
    45661 tattactgtt attgctggag ttatagcaat tataaaaaac tggggcgcaa ttactgactg
    45721 gtttaaggga atatggagta aattcactga ttggttgggt ggtacttggg aaagtataaa
    45781 agaaggtgcc tcatcagttt gggatggagt taaagaaacc tggtctggat ttgtagattg
    45841 ggttcaagat atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt
    45901 tgaaggagct tccaacatct ggcaaggagt ccaagagact tggcaagcat tcgttgattg
    45961 ggtttcaaat atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt
    46021 aggaattgtt caaataccat ggaccttaat aacgtcattg attcaagccg gtattaatat
    46081 tatcgtgggt atttttgatg tagctggaca gttattaggc gcagcttggc aagctgtttg
    46141 gacacctatt tctgatttcc ttaaaaacac ttgggatact atgacacaat gggtaagcat
    46201 cgcttggaat ggaattgtaa ctacattcca tactatattt gatccagtag tggcatggtg
    46261 gaatggtata tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc
    46321 aacagcttct agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc
    46381 tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa
    46441 ttcaatcagc tctacagcat caaatatctg gaatagcgtg actagtagta taagcaatgc
    46501 tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggatcag
    46561 cggaatttgg aacggtatca aaaacactgc tttgaatctt tggaatggaa ttacaagcac
    46621 tattagctct aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac
    46681 aggtattgtt tccgacatat tcaataatgt taaaagtaca attgctaaca tttgggaagg
    46741 tatcaaaaag actgttagcg ctccaattga ttggatcaga gataaaatca gtagtatctt
    46801 tgataatttg aatatttcta taccacatat tccgttacca cattttaaat tgagcggaga
    46861 attcaatcca ttgaagggga aaatcccaac gttgggtgtt gattggtatg cgaaaggtag
    46921 tgtgtttaat tctccgaata ttatcggtgt cggcgaagca ggacctgaag cagttttacc
    46981 tttgaaaaga tctgtgctgc aagaaattgg tgatcgtatc ttgagtagca catcagtttc
    47041 atctagggca caaacgattc aacctgtgaa taactacgaa ttcaatttca caattgatgg
    47101 taacgcagat gaggttacta tgaagcaaac aactcaacaa atcattgata gcattacaaa
    47161 agttcaaaat gataatgctt cggcatggcg ttaaacagga gagtatttct cctgtttttt
    47221 tagtattaaa aaggatgtga aaaaatgact gattgtatac attctataat cgatggattt
    47281 cctgattatt tgcataaatt ggctttagct gaaagaccaa ccataccttc tccaaaaaga
    47341 cagagagttg aaacttctgt tttaggaagg ttaggtggct tagtacaaga ttactcgttt
    47401 gaagacatgt cgtttacatt gcactataac tatttagagg atgtggaaga ccatcaagcg
    47461 ttcaagcaat cgttttatat catgcgtcat tggttaaatt atgcaaagaa attagaattc
    47521 tctgatgatc ccaacgtcta ttatgttatc cagactatcg atattgggga tgcagaaaac
    47581 gatattgttg aatggggaga gttcgatgta aatattactg cgaaaccatt cgcaagagtt
    47641 caagaagatg tacctataac cgtagataaa ccacagtcat ttaacttgct gaataatagt
    47701 ttagaagaaa gttttccaaa gattatcatc actccttcag ctacttcatg ccagttcatc
    47761 ttaaatgatt atgtgtttag ttttgaaggc ttagtagtag gaactgacgt agtcattgat
    47821 agtgatttga tgctttgcta cgaagagcaa tcggacggag atattttaga tcggtccaac
    47881 aaaatgaaga ccatgcaata tccgacattg caagtggata ttaattattt taattgtact
    47941 ggtttgagca aaatacaaat ttatcgtaat gggttaaggt aggtgaaata gatgatcgat
    48001 aatttaataa ctatttacga taaaaacgac gcgaataatt tagctgaaca tttatatgat
    48061 acgcaaggtt taggcgcttt gtcagactgg ttaacagcta ctgttagcaa taaattaaac
    48121 ggagccgaga tatttcaggg tacttatcca ataagcggaa ctaatgcaga tttgattgta
    48181 gaaggacgta ttattcagtg ttatgtagat gaaaatcgag caaaacagcg tctacggatt
    48241 tattatgcaa agacttctgt aataggcaat acgatagaag taaaagctga acctattttc
    48301 aatgatataa gaaaatcggt gttgaataaa tatgacagtg gaacagaaaa gatcactgct
    48361 agtcaggcat ggcaaaacgc aaaaacttta gcgaaaccag ttattccttc acagttttct
    48421 ttctcgtcat tagtagatac gcttgctaat gtgaagatag aaaaggcgaa ttttttagaa
    48481 ttctttggtg gaaaagaggg atctattcta gatcgatttc atggggaatt tctaaaagat
    48541 aataacacat tacgtcatga aaaaaggcta ggcacggatc ataaaatcaa agcgatttat
    48601 actaaaaact taactggtct tgacttagag attgatgctc aaagtgtttt agttggagtt
    48661 tatccattca ttagcagctc ttcagaagga gaagacgaga tcactctacc agaagaagtt
    48721 attttcacgg attacgtgga tgattatcct gctggatatg tttcttttgt tgattttaaa
    48781 gacaaagcga ctgatgtagc cacattaagg gaagctgcta aagactggtt gaaaacaaac
    48841 atagataaac aaaaaccaca agtgagtggt tcgattgaat tagtaccatt gaggcatcaa
    48901 agaggctatg aaaaatttgt tgatctagaa aaagtttcga tgggtgacgg agtagatgtg
    48961 tatcatccac agttaaaagt gaatatgtca gcgagaattg tggaatatac gtttaatgtt
    49021 ctaaccaatt catacgataa attagttgta ggaaacgtca aaacaaactt cttagaaaac
    49081 acagagaata atgtcagcaa tttgattaat gatgccattg atcaattgaa aaacggtggc
    49141 gaaatcagtg atttactcaa tgatattgta gatcatcaaa ctgatatgat tactggtcaa
    49201 aatggtggtt atgttttatt agatcctaaa gaagcgccta gtcgtatttt gattatggac
    49261 acaccagata agaataccgc aaggaatgtt ttacaaatca acaatgctgg tattggtttt
    49321 tctaaaactg gcattaatgg aacatatgaa acggcatgga cgttagatgg cggattcaat
    49381 gcctcgttta ttacggctgg tgaaatagta ggaattacta ttagaggtac tacattaatt
    49441 agtgatggtg ctgattatag aacaagtatt gctaatggca aaatgacttg gtactcaaaa
    49501 aaagttaaca aagatattat ggagctagaa gcacgtgatt atgtaagtgc tgatgccggt
    49561 attgtatcat acaccatgaa aactggtggt ggtttcatga ttagaaatcc acagggtaac
    49621 ttggttttta gtacgtggga taatggtaat aacagaccgt ttctatcttt tggtgcgccc
    49681 aatttcaggt atagcaatgc tagttatgta acttctggcg acggtagttc tttaagcatt
    49741 aatggtagtg cgggtaactc atgggaattt aaggtagctg gtaggactat gaaatttact
    49801 agtgatggta tgctaacgtt accaggttgt ttttttggtt catgggaaga tgggaaactt
    49861 gctaggtttg aacaatcaac ggtacaagta tataaagatt ttactgttag aggtactaaa
    49921 aactcaactg taccaacaga acattatgga caacgactat tgaacgctta tgaaactcca
    49981 gaatattatt tcgctgatta tggggaagcc gttacaggtg acgatggtaa agttcgtgtt
    50041 gatattgacc ccatgtttgc tgaaacagta aatctaagtc ggtatatgac acatgtgaca
    50101 cctacagaac tagttttgtg tgctgttact catgaagata ttgaccattt catcattgaa
    50161 actagtaagc caaacgtatt agttagatgg aatttagtgg cacaccgtct agggtatgaa
    50221 gatattagat taaaagagga tacagcatat gatagcacag tgcttgacca aaaacgtttt
    50281 taaaacgaag acaaggaggt atataaatgg ctagcagttt atataatttg gctttagatt
    50341 tcagcaaaga attaaactac accaaagcta ttatggctcg tcaaggtgat aaagggatta
    50401 cggtgacggt taaaccgttt ctaaatggct tgcagatgga tacgagtggc ggaacattta
    50461 ctttaaaagg aacaacacca tctaaccgtt acgtagataa tgttgcaact agtgtaacta
    50521 gtgaagaagt cacgttttct cttgatggca catttatgag tgaagcagga tattataaac
    50581 actgctacgt agaatataga aaagacaatc aaattttaac aacgcaagat atcatttttt
    50641 tctcactagg agtgtctgac atttcgcaag gccaagccga tgaatatgtt tcgcaattag
    50701 aagagttgat tcgaaagtac aacgaaactt ttgatgcttt tatggctgaa atcaaaggta
    50761 gagtggatag cttaaatcaa cagattactg atttaactgg tcaagctaaa acgctacaag
    50821 acaagttaga tgctctgaaa gaagaaattt ctaagttagg taacttacaa gtgatgtaca
    50881 gtaacagcat cgacttcggg ggatatgatt atagcggtaa gccgaattta atgtccaaac
    50941 taaaatcgag cgattttaac gttggttacc acgggtcact aactttggat aacgaaaagt
    51001 tacattttac ttctgatggt acaggaagca ttattatgtt tacgcgtatt aatacacctc
    51061 agcttactag tgggaaaacc tatactctga gtgcgaaagt tcgatttgat gaaggaacta
    51121 caggagctat tgataaatta cgtttggtgt atcgtacatc accaggagaa aagatattat
    51181 tggaagcaaa tagtacaaat attacaacag atgatgtagg gaaagaaata acaatcaaag
    51241 gtacagctaa cgttaattat caaatcacaa atttagatcg attttatatg agtattagct
    51301 ttgttgacag ggataaaata aatggcggat ttaagttgta cgacatcaaa atcgaagaag
    51361 gctcaacagc cacaccgtac cagcctaatc tacttgatgc accgtattat ttgagtaagg
    51421 tggctttggg tgagaatcta attaaaccag aatcacaaca accagttact aatagtaact
    51481 atcttattaa cacctataac attaaaccaa tggtaaaagg taagaagtat accatcacac
    51541 ttgaaggaac taagccagca acacaggttt ttagaccatt tttcacacga gcaacaggag
    51601 atgcatggga ggttggtgac ttacaaccag tagaaggctt aactaatgtg tggtctaaaa
    51661 catttacagc agcagatgac tcacacccta ctaccccaca agtgcagatt tatcaagtac
    51721 caagcacaag tgtaggacaa tgtacaatta agtggttaaa actagaggaa ggcaatactc
    51781 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    51841 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    51901 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    51961 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    52021 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    52081 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    52141 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaacgcagtg
    52201 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    52261 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    52321 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    52381 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    52441 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    52501 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    52561 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    52621 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    52681 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    52741 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    52801 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    52861 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    52921 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    52981 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    53041 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    53101 gaaaaagcag ataaaatcat tagcaaagaa gagagcgcat cttaattgat gtgcttttta
    53161 ttttgattca aggagttgtc acatgattaa tttaggggaa tggggagcga tagcaggatc
    53221 aataaccgct atcgtttctt tgattttatt agtaataaaa ccaattactg catctttctc
    53281 gaagattact gagactcttt caaaagtaag tcacaattta gatttgctga ctaaagattt
    53341 agaatcgagc aaatcagatc gattgatgat tcatgaagaa ctaaagaaac acgatgaaag
    53401 attagataca catgcagaaa aattggtaga acacacgcaa caaattaaaa cttt
//
LOCUS       DACEJZ010000007        50661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEJZ010000007 DACEJZ010000000
VERSION     DACEJZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.007"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1125)
                     /locus_tag="IW656_000410"
     CDS             complement(<1..1125)
                     /locus_tag="IW656_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0836140.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILNKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKY"
     gene            complement(1279..1809)
                     /locus_tag="IW656_000411"
     CDS             complement(1279..1809)
                     /locus_tag="IW656_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836141.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1908..2258)
                     /locus_tag="IW656_000412"
     CDS             complement(1908..2258)
                     /locus_tag="IW656_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0836142.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2415..3944)
                     /gene="ahpF"
                     /locus_tag="IW656_000413"
     CDS             complement(2415..3944)
                     /gene="ahpF"
                     /locus_tag="IW656_000413"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0836143.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3954..4517)
                     /gene="ahpC"
                     /locus_tag="IW656_000414"
     CDS             complement(3954..4517)
                     /gene="ahpC"
                     /locus_tag="IW656_000414"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0836144.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4661..5323)
                     /locus_tag="IW656_000415"
     CDS             complement(4661..5323)
                     /locus_tag="IW656_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0836145.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5507..7153
                     /locus_tag="IW656_000416"
     CDS             5507..7153
                     /locus_tag="IW656_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0836146.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7144..8226
                     /gene="uxuA"
                     /locus_tag="IW656_000417"
     CDS             7144..8226
                     /gene="uxuA"
                     /locus_tag="IW656_000417"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0836147.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(8334..9887)
                     /locus_tag="IW656_000418"
                     /pseudo
     CDS             complement(8334..9887)
                     /locus_tag="IW656_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            10035..11176
                     /locus_tag="IW656_000419"
                     /pseudo
     CDS             10035..11176
                     /locus_tag="IW656_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(11302..12381)
                     /locus_tag="IW656_000420"
     CDS             complement(11302..12381)
                     /locus_tag="IW656_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0836148.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(12484..12924)
                     /locus_tag="IW656_000421"
     CDS             complement(12484..12924)
                     /locus_tag="IW656_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0836149.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(12981..13391)
                     /locus_tag="IW656_000422"
     CDS             complement(12981..13391)
                     /locus_tag="IW656_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0836150.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13571..14005)
                     /locus_tag="IW656_000423"
     CDS             complement(13571..14005)
                     /locus_tag="IW656_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0836151.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(14105..14953)
                     /locus_tag="IW656_000424"
     CDS             complement(14105..14953)
                     /locus_tag="IW656_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0836152.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            15342..17108
                     /locus_tag="IW656_000425"
     CDS             15342..17108
                     /locus_tag="IW656_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0836153.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            17210..18469
                     /locus_tag="IW656_000426"
     CDS             17210..18469
                     /locus_tag="IW656_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0836154.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            18616..19917
                     /locus_tag="IW656_000427"
     CDS             18616..19917
                     /locus_tag="IW656_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836155.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            19920..20771
                     /locus_tag="IW656_000428"
     CDS             19920..20771
                     /locus_tag="IW656_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836156.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            20784..21599
                     /locus_tag="IW656_000429"
     CDS             20784..21599
                     /locus_tag="IW656_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0836157.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            21750..22916
                     /locus_tag="IW656_000430"
     CDS             21750..22916
                     /locus_tag="IW656_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0836158.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            23146..23793
                     /locus_tag="IW656_000431"
     CDS             23146..23793
                     /locus_tag="IW656_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0836159.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            23877..24821
                     /locus_tag="IW656_000432"
     CDS             23877..24821
                     /locus_tag="IW656_000432"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0836160.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            24956..26407
                     /locus_tag="IW656_000433"
     CDS             24956..26407
                     /locus_tag="IW656_000433"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0836161.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            26428..27264
                     /locus_tag="IW656_000434"
     CDS             26428..27264
                     /locus_tag="IW656_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0836162.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            27264..27923
                     /locus_tag="IW656_000435"
     CDS             27264..27923
                     /locus_tag="IW656_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0836163.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            28049..28738
                     /locus_tag="IW656_000436"
     CDS             28049..28738
                     /locus_tag="IW656_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836164.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            28744..29700
                     /locus_tag="IW656_000437"
     CDS             28744..29700
                     /locus_tag="IW656_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0836165.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            29744..30256
                     /locus_tag="IW656_000438"
     CDS             29744..30256
                     /locus_tag="IW656_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0836166.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(30512..31060)
                     /locus_tag="IW656_000439"
     CDS             complement(30512..31060)
                     /locus_tag="IW656_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0836167.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(31149..32330)
                     /locus_tag="IW656_000440"
     CDS             complement(31149..32330)
                     /locus_tag="IW656_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0836168.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(32566..34074)
                     /locus_tag="IW656_000441"
     CDS             complement(32566..34074)
                     /locus_tag="IW656_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0836169.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPANVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            34233..34424
                     /locus_tag="IW656_000442"
     CDS             34233..34424
                     /locus_tag="IW656_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836170.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            34627..35544
                     /locus_tag="IW656_000443"
     CDS             34627..35544
                     /locus_tag="IW656_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0836171.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            35537..36610
                     /locus_tag="IW656_000444"
     CDS             35537..36610
                     /locus_tag="IW656_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836172.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            36758..37276
                     /locus_tag="IW656_000445"
     CDS             36758..37276
                     /locus_tag="IW656_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836173.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            37257..37649
                     /locus_tag="IW656_000446"
     CDS             37257..37649
                     /locus_tag="IW656_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0836174.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            37725..38660
                     /locus_tag="IW656_000447"
     CDS             37725..38660
                     /locus_tag="IW656_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0836175.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            38793..39572
                     /locus_tag="IW656_000448"
     CDS             38793..39572
                     /locus_tag="IW656_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0836176.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            39565..39882
                     /locus_tag="IW656_000449"
     CDS             39565..39882
                     /locus_tag="IW656_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0836177.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            40126..41736
                     /locus_tag="IW656_000450"
     CDS             40126..41736
                     /locus_tag="IW656_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0836178.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            41893..42741
                     /locus_tag="IW656_000451"
     CDS             41893..42741
                     /locus_tag="IW656_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0836179.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            42972..43451
                     /locus_tag="IW656_000452"
     CDS             42972..43451
                     /locus_tag="IW656_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836180.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            43484..45712
                     /locus_tag="IW656_000453"
     CDS             43484..45712
                     /locus_tag="IW656_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0836181.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            45841..47484
                     /locus_tag="IW656_000454"
     CDS             45841..47484
                     /locus_tag="IW656_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0836182.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(47557..48102)
                     /locus_tag="IW656_000455"
     CDS             complement(47557..48102)
                     /locus_tag="IW656_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0836183.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(48125..48610)
                     /locus_tag="IW656_000456"
     CDS             complement(48125..48610)
                     /locus_tag="IW656_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0836184.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(48600..49412)
                     /locus_tag="IW656_000457"
     CDS             complement(48600..49412)
                     /locus_tag="IW656_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0836185.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(49551..>50661)
                     /locus_tag="IW656_000458"
     CDS             complement(49551..>50661)
                     /locus_tag="IW656_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0836186.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        16788 a         9227 c         9726 g        14920 t
ORIGIN      
        1 atatttgaga acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga
       61 atagtactgt ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat
      121 ttctccacgc gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc
      181 aatctgagca ccaaaataac ctccaagcaa tttgttcaaa atcgctacaa gcgtcaatat
      241 agaaataaag agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac
      301 aaaaaatact ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc
      361 tttaaccttt gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag
      421 cccaacaaga tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat
      481 agaagaattt gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac
      541 taataagaaa ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc
      601 attgctagaa gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa
      661 aatgtcatct accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt
      721 tagttctttt aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa
      781 gaaaatcgct tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac
      841 cgggaagatg atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag
      901 agataaatca ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa
      961 gtcatggact agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc
     1021 aacaagtaat tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat
     1081 cgttgttgaa atcaatatca ggcataaaat accaataaat tccattctca tcattccttt
     1141 gtataaaata tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac
     1201 tcacaaaaaa accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat
     1261 tttaataact aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat
     1321 ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact
     1381 tttgaatgga ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac
     1441 actccaactt ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat
     1501 aacagctaat ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac
     1561 gatcagagct aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt
     1621 tttcctttta aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc
     1681 ggtgatcaga taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc
     1741 agaaataata taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt
     1801 ctcaaacata taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa
     1861 attaaatgtt acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg
     1921 atcttttcca tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt
     1981 tctttcatca agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc
     2041 aaggaacgtg aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt
     2101 ttttgtagtt gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca
     2161 tctacttcct cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg
     2221 cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt
     2281 caaattattt actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa
     2341 aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct ctgacttttt
     2401 acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct
     2461 cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg
     2521 aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc
     2581 atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag aatgaatact
     2641 cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct
     2701 acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca
     2761 agagagacta gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga
     2821 acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca
     2881 ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa
     2941 gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca
     3001 cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta
     3061 ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg
     3121 taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta
     3181 ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca
     3241 agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca
     3301 ccgccaatca ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg
     3361 ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca
     3421 tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc
     3481 ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc
     3541 tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt
     3601 tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta
     3661 atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa
     3721 acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg catcgaatct
     3781 cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt
     3841 ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt
     3901 gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc
     3961 tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga
     4021 caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca
     4081 ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga
     4141 tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata
     4201 cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct
     4261 gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca
     4321 acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa
     4381 acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt
     4441 tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt
     4501 ttcccaatca atgacatagt aaaacctcca ataataatta tttatttata attattgtaa
     4561 tttagttgga gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac
     4621 gaaggttaat tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg
     4681 gataacgttg agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt
     4741 gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt
     4801 ccatgatcgt ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta
     4861 aatgggtatt atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac
     4921 aagcttcatg aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg
     4981 ctttttcttg ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg
     5041 aacaacattc aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg
     5101 cagaaacaag attaattttg gaaacatacg taccactttg aggtatcgta tacacgagtt
     5161 cttgctgacg tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt
     5221 cctccagtgc cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt
     5281 tacgaatcgc ttgataagct tgtgattgca agctgctttt catctttatt cctccaaaat
     5341 catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta
     5401 actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa
     5461 attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa
     5521 caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga
     5581 ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg
     5641 aaatctttat cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt
     5701 gtccactggt gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca
     5761 agattttgat gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg
     5821 tatattagct gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga
     5881 taagatgatt cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga
     5941 aaaaggttat cgattgaaag atacaaataa taaatatata ggaccgattg cagtcgatat
     6001 aaaaaatggt ccaaagaagc catcacatac aatgagtatt cttgctgtgc tactttataa
     6061 acgtttttta tctggagctt ggccgattgc acttgtctct acagataatt tttcacaaaa
     6121 cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa aggaaggtta
     6181 tgtacaaaat acatttatcc aatacttatc gaatgaagaa attgttgcgt ttccatggac
     6241 gatgattgat cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt tggcaaaaga
     6301 gggcttttca aatatgtcga ttttccaaac cgcaaaagga aataatattg ctccatttgt
     6361 caatacagag gacatccatt atttaatcat ggaagatcat tttcccaatg gacgtccgaa
     6421 attcgaaaaa gctggggtat tcttgactga tcgtgcaacg gtagataaag cagatacgat
     6481 gaaggtgacg acttgcctta atcctttgca tacgtcttta gctattatgg gttgtttact
     6541 ggggtatagg agcatagctg aggagatgaa agatccagat ttatcgctgc tagtttctga
     6601 aatcgggtac aatgaaggcc tcccagttgt ggagtctcca ggtatcattg atccaaagat
     6661 ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca aatattccta atatgcctca
     6721 gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt tatggtgaga cgattaaaaa
     6781 atatatggaa atagaagtta aagacccgca gacacttgta ttcataccat tgactctcgc
     6841 tgcttggctt cgatacctta ttggcattga tgatcaagga aaaccattca tcccgagtcc
     6901 agacccacta ttgacagacc ttcagcataa gctttccgat attcaactgg gtacgcaaga
     6961 tacatcacat attcatgaga aaatggctcc tattttgtca aataaagaaa tttttggtgt
     7021 agatctttac caagcagagc ttggcagaaa aatagaagaa atgttcatca agatggtagc
     7081 aggaccaaat gccgtacgaa aaacattaca ggattactta caagaacatg gaggaaattt
     7141 atcatggaaa tgacttttag atggtacgga tcacaggaag aaaagattac cttagatgaa
     7201 atcagacaga ttcctgcggt caaagggatt gtaggcacat tgatggatat accagtagga
     7261 gaggtatggc ctagggagag gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta
     7321 acactaaaag taattgaaag cgtcaatgtg catgatgcca ttaaaattgg attaccttcc
     7381 cgtgagcagt atatcgaaaa ctataagcag acaatccgaa acttggcaga atatggaata
     7441 gaagtcatct gctataattt tatgcctgta tttgactggg tgaaatctga ccttgactac
     7501 cgcttagaag acggctcatc cacgttagct tttatatctg atgatattcc agatgatcca
     7561 agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat ttggattgcc gggatgggaa
     7621 cctgagcgct tagctcctat caaatccttg cttgaagcct atgcatcagt agacgaagaa
     7681 aaacttcgag aaaattttgc ctattttctc caatcaatca tcccaacatg tgaagaggta
     7741 ggggtcaaaa tggctgtaca tccagatgat ccgccatact cgatttttgg attagctcga
     7801 atcgtaaaga atcgagaaga tttagattgg ttgtgtcgca tagtagacag cccttataac
     7861 ggaatcactc tttgtacagg gagtattgca gaagatccag aaaatgatgt atatgggatt
     7921 ttagcagaat tttgccaaag agatcgaatc ccatttgcac atgttcgaaa tattaaattt
     7981 ttagctaatc gagatttcta tgaggcacca cataaatccg aatacggttc attagacatg
     8041 taccgcatac taaaaacaat gtatgacaac ggatttaacg gatacatccg tcctgatcat
     8101 ggaagaatga tctggaatga gacaggaaga ccaggatacg gcttatatga tcgagcatta
     8161 ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat
     8221 aattagtgaa aaaaactgta attttattat gtaaataaga aggatgttca aattcttgat
     8281 acataaatcc ggaggttgtt aataagcatt cggatttatg tatcttattt ggtctaggaa
     8341 gtaacttgat tttctttttt cgattgatcc atgagtgctt cttcttttaa ttcaaagata
     8401 tattctgaga tcctcataat cacttttttc cagtcttctg gtgtgatgat tccttttaaa
     8461 atatagattt ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct gataacgatg
     8521 ttgcagttct ccataaaatc attttgtatt ttgatcgatt catttgaaaa aatttgttct
     8581 atcttaacga tcagatcttt actgatataa aaatcacgcg tatagttcac accaatcaca
     8641 attcgcggct gttttttgta gcgatcatac aaatgtacta ggtcttcaac catccattgc
     8701 acattttcct tttctaaaat aactggtaac aagtgttgat tttgttctct gaaatccata
     8761 taaaattgtt caatggcggt ttggacttct ttcaatacgc tatgttcttg ataaagcatg
     8821 aaagtaggaa ggggcgtatt tggttgattg aagttaagaa atccattata aagtttattt
     8881 cgtgcgaatg ccaatgagta aagaatcttt tctttttcag gtataaacaa atcaaataca
     8941 gtacaaaggg actccaccat ttctcttgaa taaagaacat gaggaacgtt tgcttttttt
     9001 aaaagtttgt attgctccaa gcttgtttct tccgattcga ttttcgtgat agaaagtcga
     9061 gctaaaatat tcactaagat gcgttgttcg cgacaaattg gcagattagg tcgcaaaata
     9121 tctacagaga gatcaataaa aaagtcaata tattcatact ctgtgaattc tttttgtacg
     9181 tctttcacag aagttgcagg aaaattctct ttacatattt ttaatagaaa atataattta
     9241 tctatcatac cgctatttag tcggtcaagt acttcttttt taaaaaaagt attcatcata
     9301 aaagccacta cttgcggatc ttcatcataa aattctgtag cagtacttga aaagatgttc
     9361 caataaatat ctaatgctgc atattgaatt tgtacctctt ttccagaaaa agactttctg
     9421 ccattattag aaaaggagat agacaaaccg tactatgcta aaaatgcatt aatctttttc
     9481 attttcgtat acaaataaga ggcactatag tttgtttgct gactgaaact gataatcgaa
     9541 tttgtctgac agctgaacaa agaagtcaag atctgataac tgactgattc gttgcagtat
     9601 cttccaaata gacgataata gcagtaagcc ggatcaattc cttcaaaatt caactttaaa
     9661 aaaggttttt gcttatcaat atgcagttct gttagattga gacgctcaaa gtcttgttct
     9721 aattctttga tgtatagtaa aaaatttgat tcagacatat ggaaatgatc tgctaaatat
     9781 tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa aaatatctaa ttttgtattg
     9841 ctttttttct tcaagataaa cgttttaatc gcttcttgag tttgcatgaa agaggctcct
     9901 ttttacttaa attgttaaaa cttgaaaata ttactgtttt tttcataaat ttagacaaaa
     9961 aaacagtaaa catataggta gaatctctac tatcttattt cttgcataca aaaaaatcaa
    10021 ggagtgagag agttatgaga tattcaacca tcttaatatc aacatttgca gccttaagct
    10081 tatcgcttac tataaatcag acagtccaca cagaggagac agaaacagcc aaatggacag
    10141 aaaccaagtg ggccaacgtc aatcgaatta cctttcagga tgaattttca tccttcaatt
    10201 caggagtcgc aaaattaaga aaaggacaaa cagcgaccat caaagcaaaa attgactatt
    10261 cgggagactt agcacctata ctacaatcag ctgttacatt tgagaatgtt gatcccactt
    10321 tgtcgattgg ctatgacgag aacactttga ctatccaaca caagcaagcg atatttacct
    10381 tcaccgttac attaaatcag gacttgttaa aaccagcgct ttttactgtc aaggtatcag
    10441 atggactacc gaacgatcat catttaacgc cgtatagtca gcggcagaca attgagcaag
    10501 attcagccat cgactcagga ggagatcttg ttgaagaacc aacagataaa ccagaaaatg
    10561 aaaataagcc agaagtaccg cctacagaaa atccagacgg agaacagaaa ccggaaatag
    10621 aacctggaga agaacccgat acagaaactc aaccagagcc agacaataaa tcaaaacctg
    10681 aaataacccc tggagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc
    10741 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
    10801 aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc
    10861 aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta
    10921 aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa
    10981 tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg
    11041 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
    11101 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
    11161 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
    11221 tatccagaaa atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
    11281 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
    11341 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
    11401 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
    11461 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
    11521 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
    11581 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
    11641 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
    11701 caacgtattt aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac
    11761 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
    11821 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
    11881 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
    11941 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
    12001 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat
    12061 gtcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
    12121 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
    12181 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
    12241 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
    12301 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
    12361 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa
    12421 aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca
    12481 gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg
    12541 cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc
    12601 ttctgcttgt ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc
    12661 atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg
    12721 agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt
    12781 cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag
    12841 gaggggagtg tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg
    12901 attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata
    12961 gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca
    13021 gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct
    13081 tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca
    13141 ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc
    13201 tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt
    13261 tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta
    13321 cgatcgggat tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac
    13381 atttttttca taaaattacc acctttaatt atattttata caattgaatt ttacacaata
    13441 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
    13501 agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc
    13561 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac
    13621 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
    13681 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
    13741 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
    13801 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac
    13861 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
    13921 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
    13981 aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
    14041 agattctttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac
    14101 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
    14161 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
    14221 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
    14281 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
    14341 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
    14401 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
    14461 taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
    14521 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
    14581 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
    14641 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc
    14701 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
    14761 taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta
    14821 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
    14881 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
    14941 tcgtttcttt catttattca cttccttaat tcattaataa tactcttatc ttattataac
    15001 ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta
    15061 tgtttcattc ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag
    15121 aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag
    15181 actaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
    15241 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac
    15301 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
    15361 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
    15421 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca
    15481 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
    15541 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc
    15601 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
    15661 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
    15721 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
    15781 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
    15841 atccagaccg agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt
    15901 acttgacgga ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca
    15961 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
    16021 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
    16081 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
    16141 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
    16201 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
    16261 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
    16321 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
    16381 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
    16441 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
    16501 tgaattatgc atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
    16561 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
    16621 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
    16681 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
    16741 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
    16801 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
    16861 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
    16921 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
    16981 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
    17041 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
    17101 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
    17161 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
    17221 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
    17281 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
    17341 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
    17401 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
    17461 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
    17521 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
    17581 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
    17641 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
    17701 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
    17761 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
    17821 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
    17881 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
    17941 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
    18001 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
    18061 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
    18121 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
    18181 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
    18241 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
    18301 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
    18361 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
    18421 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
    18481 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
    18541 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
    18601 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
    18661 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
    18721 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
    18781 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
    18841 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
    18901 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
    18961 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
    19021 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
    19081 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
    19141 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
    19201 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
    19261 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
    19321 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
    19381 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
    19441 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
    19501 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
    19561 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
    19621 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
    19681 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
    19741 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
    19801 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
    19861 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
    19921 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
    19981 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
    20041 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
    20101 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
    20161 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
    20221 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
    20281 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
    20341 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
    20401 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
    20461 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
    20521 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
    20581 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
    20641 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
    20701 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
    20761 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
    20821 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
    20881 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
    20941 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
    21001 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
    21061 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
    21121 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
    21181 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
    21241 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
    21301 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
    21361 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
    21421 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
    21481 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
    21541 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
    21601 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
    21661 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
    21721 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
    21781 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
    21841 acggagatac ggacactcct acccaaatca aaatcaatat ccatgaagaa atcccaatga
    21901 aagagattac aagtaacgca tctttggcac taggagaagc ctatatggat catcgaatcg
    21961 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
    22021 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
    22081 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
    22141 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
    22201 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
    22261 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
    22321 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
    22381 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
    22441 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
    22501 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
    22561 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
    22621 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
    22681 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
    22741 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
    22801 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
    22861 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
    22921 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
    22981 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
    23041 tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
    23101 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
    23161 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
    23221 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
    23281 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
    23341 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
    23401 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
    23461 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
    23521 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
    23581 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
    23641 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
    23701 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
    23761 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
    23821 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
    23881 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
    23941 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
    24001 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
    24061 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
    24121 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
    24181 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
    24241 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
    24301 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
    24361 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
    24421 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
    24481 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
    24541 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
    24601 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
    24661 atctgctatg tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg
    24721 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
    24781 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
    24841 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
    24901 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
    24961 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
    25021 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
    25081 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
    25141 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    25201 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    25261 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    25321 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    25381 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    25441 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    25501 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    25561 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    25621 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    25681 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    25741 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    25801 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    25861 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    25921 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    25981 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    26041 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    26101 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    26161 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    26221 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    26281 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    26341 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    26401 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    26461 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    26521 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    26581 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    26641 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    26701 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    26761 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    26821 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    26881 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    26941 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    27001 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    27061 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    27121 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    27181 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    27241 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    27301 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    27361 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    27421 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    27481 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    27541 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    27601 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    27661 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    27721 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    27781 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    27841 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    27901 gttctatgca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    27961 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    28021 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    28081 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    28141 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    28201 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    28261 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    28321 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    28381 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    28441 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    28501 gcaaatggag cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag
    28561 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    28621 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    28681 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    28741 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    28801 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    28861 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatcgaat cgctgtgatc
    28921 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    28981 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    29041 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    29101 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    29161 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    29221 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    29281 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    29341 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    29401 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    29461 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    29521 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    29581 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    29641 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    29701 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    29761 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    29821 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    29881 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    29941 tgcattagtc tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    30001 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    30061 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    30121 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    30181 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    30241 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    30301 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    30361 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    30421 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct
    30481 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    30541 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    30601 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    30661 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    30721 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    30781 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    30841 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    30901 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    30961 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    31021 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    31081 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    31141 tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    31201 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    31261 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    31321 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    31381 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    31441 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    31501 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
    31561 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
    31621 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
    31681 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
    31741 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
    31801 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
    31861 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
    31921 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
    31981 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
    32041 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
    32101 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
    32161 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
    32221 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
    32281 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
    32341 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
    32401 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
    32461 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
    32521 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
    32581 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
    32641 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
    32701 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
    32761 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
    32821 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
    32881 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
    32941 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
    33001 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
    33061 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
    33121 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
    33181 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
    33241 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
    33301 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
    33361 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
    33421 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
    33481 atgtcctttt tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc
    33541 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
    33601 aaaaccatct tctgagacga ttttttctac gttggctgga tagtcttcga tttctgagtg
    33661 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
    33721 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
    33781 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
    33841 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
    33901 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
    33961 aactagcttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
    34021 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
    34081 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
    34141 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    34201 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    34261 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    34321 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    34381 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    34441 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    34501 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    34561 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    34621 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    34681 aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    34741 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    34801 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    34861 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    34921 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    34981 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    35041 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    35101 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    35161 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    35221 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    35281 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    35341 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    35401 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    35461 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    35521 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    35581 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    35641 tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac
    35701 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    35761 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    35821 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    35881 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    35941 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    36001 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    36061 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    36121 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    36181 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    36241 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    36301 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    36361 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    36421 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    36481 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    36541 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    36601 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    36661 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    36721 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    36781 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    36841 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    36901 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    36961 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    37021 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    37081 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    37141 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    37201 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    37261 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    37321 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    37381 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa
    37441 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    37501 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    37561 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    37621 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    37681 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    37741 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    37801 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    37861 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    37921 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    37981 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    38041 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    38101 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    38161 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    38221 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    38281 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    38341 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    38401 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    38461 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    38521 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    38581 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    38641 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    38701 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    38761 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    38821 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    38881 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    38941 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    39001 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    39061 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    39121 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    39181 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    39241 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    39301 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    39361 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    39421 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    39481 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    39541 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    39601 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    39661 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    39721 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    39781 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    39841 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    39901 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    39961 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    40021 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    40081 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    40141 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    40201 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    40261 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    40321 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    40381 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    40441 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    40501 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    40561 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    40621 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    40681 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    40741 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    40801 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    40861 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    40921 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    40981 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    41041 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    41101 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    41161 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    41221 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    41281 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    41341 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    41401 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    41461 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    41521 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    41581 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    41641 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    41701 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    41761 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    41821 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    41881 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    41941 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    42001 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    42061 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    42121 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    42181 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    42241 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    42301 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac
    42361 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    42421 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    42481 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    42541 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    42601 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    42661 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    42721 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca
    42781 tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta
    42841 tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca
    42901 tatagtttag taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag
    42961 gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag
    43021 actatgataa agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg
    43081 gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata
    43141 ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag
    43201 cgatcttagc tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt
    43261 atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag
    43321 aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac
    43381 aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc
    43441 taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact
    43501 aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag
    43561 ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga
    43621 tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca
    43681 attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc
    43741 agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga
    43801 acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac
    43861 actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct
    43921 agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga
    43981 aatcgttcga gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg
    44041 agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt
    44101 accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa
    44161 ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa
    44221 aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca
    44281 aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa
    44341 agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta
    44401 taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac
    44461 tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga
    44521 acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg
    44581 aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat
    44641 tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa
    44701 acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag
    44761 cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc
    44821 tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg
    44881 acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt
    44941 ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc
    45001 acttagccgg caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg
    45061 aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt
    45121 tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga
    45181 atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat
    45241 tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg
    45301 ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg
    45361 gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact
    45421 tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat
    45481 tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat
    45541 ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg
    45601 ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga
    45661 cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa
    45721 agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa
    45781 ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat
    45841 atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt
    45901 ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat
    45961 tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt
    46021 gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg
    46081 gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat
    46141 cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa
    46201 gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat
    46261 aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat
    46321 tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa
    46381 gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta
    46441 gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc
    46501 tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt
    46561 gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca
    46621 caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc
    46681 tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct
    46741 tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt
    46801 cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa
    46861 gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa
    46921 atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa
    46981 agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt
    47041 cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct
    47101 ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta
    47161 gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt
    47221 agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca
    47281 caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta
    47341 acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc
    47401 ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct
    47461 cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg
    47521 aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc
    47581 gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg
    47641 aggtctgaca ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc
    47701 gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc
    47761 agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt
    47821 ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg
    47881 gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg
    47941 accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca
    48001 tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat
    48061 ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa accttctttt
    48121 tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa
    48181 gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc
    48241 aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata
    48301 aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt
    48361 gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga
    48421 tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag
    48481 cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca
    48541 aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct
    48601 tagttttcat ttctcattcc tcctttttga ataccgccag accagacact tccggtatga
    48661 tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact
    48721 gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa
    48781 ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca
    48841 attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca
    48901 atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa
    48961 gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta
    49021 acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca
    49081 gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg
    49141 aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct
    49201 aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat
    49261 tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg
    49321 aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga
    49381 tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata
    49441 acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa
    49501 aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt
    49561 tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg
    49621 attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc
    49681 caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa
    49741 tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag
    49801 tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa
    49861 aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa
    49921 acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt
    49981 aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga
    50041 gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc
    50101 tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga
    50161 aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata
    50221 gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca
    50281 gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct
    50341 tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc
    50401 atggacatca aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa
    50461 gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat
    50521 cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg
    50581 gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt
    50641 tccattaaat aaagaatagg a
//
LOCUS       DACEJZ010000008        49889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEJZ010000008 DACEJZ010000000
VERSION     DACEJZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.008"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IW656_000459"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="IW656_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0836187.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="IW656_000460"
     CDS             complement(324..893)
                     /locus_tag="IW656_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0836188.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="IW656_000461"
     CDS             1079..1981
                     /locus_tag="IW656_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0836189.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IW656_000462"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="IW656_000462"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0836190.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IW656_000463"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="IW656_000463"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0836191.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IW656_000464"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="IW656_000464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0836192.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="IW656_000465"
     CDS             8549..8734
                     /locus_tag="IW656_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0836193.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="IW656_000466"
     CDS             complement(8796..9992)
                     /locus_tag="IW656_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0836194.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="IW656_000467"
     CDS             complement(10162..11598)
                     /locus_tag="IW656_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0836195.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="IW656_000468"
     CDS             11891..12700
                     /locus_tag="IW656_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0836196.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12886..15039)
                     /locus_tag="IW656_000469"
     CDS             complement(12886..15039)
                     /locus_tag="IW656_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0836197.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15333..16181)
                     /locus_tag="IW656_000470"
     CDS             complement(15333..16181)
                     /locus_tag="IW656_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0836198.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16405..18345)
                     /gene="thrS"
                     /locus_tag="IW656_000471"
     CDS             complement(16405..18345)
                     /gene="thrS"
                     /locus_tag="IW656_000471"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0836199.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18682..18861)
                     /locus_tag="IW656_000472"
     CDS             complement(18682..18861)
                     /locus_tag="IW656_000472"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0836200.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18908..20731)
                     /gene="cadA"
                     /locus_tag="IW656_000473"
     CDS             complement(18908..20731)
                     /gene="cadA"
                     /locus_tag="IW656_000473"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0836201.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21009..23288
                     /locus_tag="IW656_000474"
     CDS             21009..23288
                     /locus_tag="IW656_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0836202.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23381..24028)
                     /locus_tag="IW656_000475"
     CDS             complement(23381..24028)
                     /locus_tag="IW656_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836203.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24207..24926)
                     /locus_tag="IW656_000476"
     CDS             complement(24207..24926)
                     /locus_tag="IW656_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0836204.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25057..26208)
                     /locus_tag="IW656_000477"
     CDS             complement(25057..26208)
                     /locus_tag="IW656_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0836205.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26425..27165)
                     /locus_tag="IW656_000478"
     CDS             complement(26425..27165)
                     /locus_tag="IW656_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0836206.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27169..27492)
                     /gene="rsfS"
                     /locus_tag="IW656_000479"
     CDS             complement(27169..27492)
                     /gene="rsfS"
                     /locus_tag="IW656_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0836207.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27549..28142)
                     /locus_tag="IW656_000480"
     CDS             complement(27549..28142)
                     /locus_tag="IW656_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0836208.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28126..28773)
                     /locus_tag="IW656_000481"
     CDS             complement(28126..28773)
                     /locus_tag="IW656_000481"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0836209.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28786..29097)
                     /gene="yhbY"
                     /locus_tag="IW656_000482"
     CDS             complement(28786..29097)
                     /gene="yhbY"
                     /locus_tag="IW656_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0836210.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29113..30222)
                     /gene="yqeH"
                     /locus_tag="IW656_000483"
     CDS             complement(29113..30222)
                     /gene="yqeH"
                     /locus_tag="IW656_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0836211.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30219..30746)
                     /locus_tag="IW656_000484"
     CDS             complement(30219..30746)
                     /locus_tag="IW656_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0836212.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30926..31711)
                     /locus_tag="IW656_000485"
     CDS             complement(30926..31711)
                     /locus_tag="IW656_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0836213.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31704..32573)
                     /locus_tag="IW656_000486"
     CDS             complement(31704..32573)
                     /locus_tag="IW656_000486"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0836214.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32574..33953)
                     /locus_tag="IW656_000487"
     CDS             complement(32574..33953)
                     /locus_tag="IW656_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0836215.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33961..34380)
                     /gene="fabZ"
                     /locus_tag="IW656_000488"
     CDS             complement(33961..34380)
                     /gene="fabZ"
                     /locus_tag="IW656_000488"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0836216.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34405..34881)
                     /gene="accB"
                     /locus_tag="IW656_000489"
     CDS             complement(34405..34881)
                     /gene="accB"
                     /locus_tag="IW656_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0836217.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34884..36119)
                     /gene="fabF"
                     /locus_tag="IW656_000490"
     CDS             complement(34884..36119)
                     /gene="fabF"
                     /locus_tag="IW656_000490"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0836218.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36138..36875)
                     /gene="fabG"
                     /locus_tag="IW656_000491"
     CDS             complement(36138..36875)
                     /gene="fabG"
                     /locus_tag="IW656_000491"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0836219.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36887..37804)
                     /gene="fabD"
                     /locus_tag="IW656_000492"
     CDS             complement(36887..37804)
                     /gene="fabD"
                     /locus_tag="IW656_000492"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0836220.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37911..38135)
                     /locus_tag="IW656_000493"
     CDS             complement(37911..38135)
                     /locus_tag="IW656_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0836221.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38188..39150)
                     /locus_tag="IW656_000494"
     CDS             complement(38188..39150)
                     /locus_tag="IW656_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0836222.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39160..39600)
                     /locus_tag="IW656_000495"
     CDS             complement(39160..39600)
                     /locus_tag="IW656_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0836223.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39906..40871
                     /locus_tag="IW656_000496"
     CDS             39906..40871
                     /locus_tag="IW656_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0836224.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40928..41158)
                     /locus_tag="IW656_000497"
     CDS             complement(40928..41158)
                     /locus_tag="IW656_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0836225.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41244..42131)
                     /locus_tag="IW656_000498"
     CDS             complement(41244..42131)
                     /locus_tag="IW656_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0836226.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42220..43443)
                     /locus_tag="IW656_000499"
     CDS             complement(42220..43443)
                     /locus_tag="IW656_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0836227.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43460..44539)
                     /locus_tag="IW656_000500"
     CDS             complement(43460..44539)
                     /locus_tag="IW656_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0836228.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(44670..46397)
                     /gene="ptsP"
                     /locus_tag="IW656_000501"
     CDS             complement(44670..46397)
                     /gene="ptsP"
                     /locus_tag="IW656_000501"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0836229.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(46397..46663)
                     /locus_tag="IW656_000502"
     CDS             complement(46397..46663)
                     /locus_tag="IW656_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0836230.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47142..49376
                     /locus_tag="IW656_000503"
     CDS             47142..49376
                     /locus_tag="IW656_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0836231.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49524..49844
                     /locus_tag="IW656_000504"
     CDS             49524..49844
                     /locus_tag="IW656_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0836232.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        14254 a        10709 c         8730 g        16196 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtgacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
    12781 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
    12841 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
    12901 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
    12961 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
    13021 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
    13081 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
    13141 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
    13201 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
    13261 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
    13321 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
    13381 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
    13441 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
    13501 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
    13561 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
    13621 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
    13681 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
    13741 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
    13801 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
    13861 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
    13921 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
    13981 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
    14041 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
    14101 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
    14161 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
    14221 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
    14281 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
    14341 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
    14401 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
    14461 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
    14521 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
    14581 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
    14641 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
    14701 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
    14761 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
    14821 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
    14881 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
    14941 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaacca
    15001 agaagtattt aatatttttt tcataaaatc tttcatcatt tcctatcctt ccgcctattt
    15061 acattcatgc ttcattgtac ccaaaaactt cttaataaaa aagaaaaaat gggcggagca
    15121 agaaattctt tacctaattt caagataaaa tcaacttttt gtttcacagc aatcacttaa
    15181 agttacatat ccaactattt ttttcattaa aaaataaact tatttttgaa aattatgttt
    15241 tcttaagtcg tgaaacactt tgatggatca tttatttgtg gaaaactacg ttttgttatt
    15301 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
    15361 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
    15421 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
    15481 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
    15541 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
    15601 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
    15661 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
    15721 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
    15781 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
    15841 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
    15901 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
    15961 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
    16021 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
    16081 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
    16141 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
    16201 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
    16261 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
    16321 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
    16381 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
    16441 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
    16501 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
    16561 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
    16621 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
    16681 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
    16741 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
    16801 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
    16861 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
    16921 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
    16981 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
    17041 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
    17101 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
    17161 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
    17221 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
    17281 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
    17341 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
    17401 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
    17461 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
    17521 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
    17581 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
    17641 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
    17701 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
    17761 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
    17821 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
    17881 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
    17941 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
    18001 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
    18061 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
    18121 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
    18181 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
    18241 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
    18301 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
    18361 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
    18421 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
    18481 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
    18541 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
    18601 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
    18661 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
    18721 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
    18781 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
    18841 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
    18901 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
    18961 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
    19021 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
    19081 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
    19141 ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag
    19201 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
    19261 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
    19321 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
    19381 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
    19441 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
    19501 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
    19561 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
    19621 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
    19681 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
    19741 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
    19801 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
    19861 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
    19921 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
    19981 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
    20041 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
    20101 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
    20161 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
    20221 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
    20281 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
    20341 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
    20401 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
    20461 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
    20521 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
    20581 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
    20641 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
    20701 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
    20761 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
    20821 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
    20881 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
    20941 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
    21001 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
    21061 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
    21121 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
    21181 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
    21241 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
    21301 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
    21361 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
    21421 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
    21481 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
    21541 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
    21601 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
    21661 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
    21721 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
    21781 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
    21841 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
    21901 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
    21961 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
    22021 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
    22081 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
    22141 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
    22201 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
    22261 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
    22321 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
    22381 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
    22441 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
    22501 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
    22561 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
    22621 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
    22681 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
    22741 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
    22801 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
    22861 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
    22921 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
    22981 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
    23041 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
    23101 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
    23161 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
    23221 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
    23281 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
    23341 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
    23401 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
    23461 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
    23521 atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat
    23581 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
    23641 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
    23701 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
    23761 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
    23821 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
    23881 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
    23941 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
    24001 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
    24061 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
    24121 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
    24181 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
    24241 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
    24301 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
    24361 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
    24421 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
    24481 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
    24541 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
    24601 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
    24661 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
    24721 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
    24781 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
    24841 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
    24901 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
    24961 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
    25021 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
    25081 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
    25141 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
    25201 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
    25261 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    25321 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    25381 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    25441 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    25501 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    25561 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    25621 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    25681 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    25741 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    25801 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    25861 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    25921 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    25981 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    26041 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    26101 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    26161 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    26221 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    26281 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    26341 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    26401 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    26461 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    26521 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    26581 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    26641 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    26701 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    26761 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    26821 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    26881 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    26941 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    27001 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    27061 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    27121 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    27181 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    27241 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    27301 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    27361 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    27421 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    27481 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    27541 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    27601 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    27661 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    27721 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    27781 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    27841 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    27901 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    27961 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    28021 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    28081 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    28141 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    28201 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    28261 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    28321 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    28381 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    28441 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    28501 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    28561 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    28621 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    28681 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    28741 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    28801 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    28861 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    28921 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    28981 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    29041 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    29101 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    29161 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    29221 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    29281 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    29341 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    29401 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    29461 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    29521 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    29581 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    29641 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    29701 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    29761 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    29821 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    29881 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    29941 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    30001 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    30061 ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    30121 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    30181 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    30241 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    30301 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    30361 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    30421 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    30481 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    30541 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    30601 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    30661 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    30721 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    30781 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    30841 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    30901 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    30961 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    31021 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    31081 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    31141 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    31201 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    31261 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    31321 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    31381 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    31441 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    31501 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    31561 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    31621 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    31681 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    31741 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    31801 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    31861 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    31921 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    31981 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    32041 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    32101 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    32161 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    32221 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    32281 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    32341 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    32401 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    32461 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    32521 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    32581 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    32641 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    32701 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    32761 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    32821 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    32881 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    32941 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    33001 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    33061 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    33121 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    33181 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    33241 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    33301 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    33361 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    33421 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    33481 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    33541 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    33601 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    33661 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    33721 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    33781 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    33841 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    33901 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    33961 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    34021 tcctttgcct attccggctg aagttttcac tttcaatatc tcaacttcaa ggtataaggt
    34081 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    34141 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    34201 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    34261 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    34321 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    34381 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    34441 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    34501 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    34561 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    34621 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    34681 tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc
    34741 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    34801 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    34861 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    34921 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    34981 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    35041 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    35101 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    35161 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    35221 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    35281 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    35341 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    35401 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    35461 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    35521 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    35581 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    35641 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    35701 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    35761 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    35821 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    35881 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    35941 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    36001 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    36061 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    36121 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    36181 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    36241 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    36301 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    36361 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    36421 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    36481 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    36541 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    36601 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    36661 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    36721 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    36781 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    36841 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    36901 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    36961 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    37021 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    37081 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    37141 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    37201 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    37261 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    37321 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    37381 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    37441 gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    37501 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    37561 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    37621 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    37681 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    37741 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    37801 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    37861 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    37921 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    37981 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    38041 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    38101 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    38161 tgctttttat ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa
    38221 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    38281 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    38341 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    38401 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    38461 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    38521 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    38581 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    38641 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    38701 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    38761 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    38821 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    38881 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    38941 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    39001 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    39061 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    39121 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    39181 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    39241 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    39301 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    39361 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    39421 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    39481 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    39541 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    39601 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta
    39661 acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga
    39721 ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc
    39781 atctatctca catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc
    39841 taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag
    39901 atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga
    39961 aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa
    40021 gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata
    40081 taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc
    40141 aattgatcca acattaccgt cctttttata accgacaaat gaattatttc agtaattata
    40201 attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat
    40261 acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt
    40321 atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc
    40381 tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa
    40441 ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt
    40501 tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga
    40561 tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag
    40621 cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga
    40681 ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt
    40741 tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca
    40801 aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc
    40861 aactaaatta gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc
    40921 ctaaaattta ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt
    40981 cgtattgctg atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag
    41041 ttactgtaca cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg
    41101 ctgactcgac catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg
    41161 gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga
    41221 ttttctctaa aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac
    41281 caaagtttga tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca
    41341 tatagtttca atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct
    41401 aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct
    41461 tttaaaatac gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc
    41521 gtctcaatca ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc
    41581 attcctgtca tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc
    41641 ccagcagaac catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga
    41701 tcataaacgg cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag
    41761 tcaccaccgg ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca
    41821 attttttctg ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata
    41881 tctgcagaaa aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct
    41941 accattgtaa agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata
    42001 gctccttctg ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg
    42061 tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa
    42121 ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac
    42181 gctagaaaca aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa
    42241 ttttattttg attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg
    42301 ataaacaacc atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat
    42361 atcataaagt ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc
    42421 aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc
    42481 gaacaatcgg ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc
    42541 ttctgtataa gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc
    42601 tttataataa agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc
    42661 taactgcagt tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat
    42721 aactaatctg gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg
    42781 gatatttttc tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag
    42841 acctagtgac tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt
    42901 ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa
    42961 tgtatcaata acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct
    43021 agacaagtat tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta
    43081 gtcttcatac atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat
    43141 cttcccaagt atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc
    43201 tttggcaacc aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga
    43261 aataaatggt acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc
    43321 ggtcgttgta aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt
    43381 gatcgatgta gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt
    43441 catgtttttc gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga
    43501 ctttttcatt cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac
    43561 ttcgctcttt tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa
    43621 tctgttggat cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat
    43681 cttctaacca gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta
    43741 aagcttctaa aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg
    43801 cacctgaata cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt
    43861 caggatgatc ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat
    43921 gaccaaacca aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga
    43981 ttcctttacg ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt
    44041 tgaaatattc tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga
    44101 tcccattgga aatagcttga atcggcacac ggataccata gctttccaac aactgttttg
    44161 aataaggtgt tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt
    44221 ttacgatagg agaaagctgg ttcgatccga taaaggaatt tcggaaatct tcctctgtag
    44281 aatgcgcatg atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac
    44341 tattgattcc gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat
    44401 aagagatacc gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag
    44461 attttgcaag catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt
    44521 cacttatata gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttgtc
    44581 tattcttact gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt
    44641 acaagccaca ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg
    44701 acttcttcca tcgtatcgca ttctttcaat gcacgatcag caagttcagc cattttcgct
    44761 gtgtccaagc gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac
    44821 tcatctagac ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca
    44881 cacatacctg cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt
    44941 aaaattgatg ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct
    45001 gccattgtgt attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca
    45061 aacttgtctg ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta
    45121 tcagatactt ccttgccttc acttatcaat ttttgttttt cttcttcaaa gatcgctttt
    45181 gctgcgcgga attctttgag tgtagctacc atagggaaca tgatacgtaa attaccatga
    45241 acagatgcac gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat
    45301 aaactgatac gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga
    45361 tatggtagtt ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc
    45421 ataccttcta atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga
    45481 gaatccatat acaggaattc agtacggtac agaccaactg cttctccacc attattatgg
    45541 acaccaacta gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca
    45601 gctgtgacag tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct
    45661 gcacctgctt tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct
    45721 tcgatcccgt tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgtc
    45781 ccaacgattg ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca
    45841 ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga
    45901 gtcaagtcat gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta
    45961 acgcctaata agtgcgctaa aatacgttta gcaacgtcac gaatatctgc agcacgctct
    46021 tgcatataag cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct
    46081 tttagcgctg attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc
    46141 atttctggat ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct
    46201 aagctttgcg ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca
    46261 gcatccaaac gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa
    46321 tccggttgaa ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct
    46381 tttagcattt caaccattat tcagataagc cttctttctt cattgtttca acgattgctg
    46441 ccattgcatc agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta
    46501 cacctaaaga cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta
    46561 agtttacatc agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt
    46621 ggatcccagt ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt
    46681 aagtgagtgt tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa
    46741 atgccaccct ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat
    46801 tcaatttcag ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat
    46861 tttataacaa acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc
    46921 attctttcct ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat
    46981 ttttagtata ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc
    47041 tcataaatct cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa
    47101 atatggatgt acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg
    47161 gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg
    47221 attactgtca gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata
    47281 tggcccaaaa tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc
    47341 gccaaatgca acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca
    47401 acaacttcaa tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg
    47461 gaatcaatat cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg
    47521 acgaaatcaa acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga
    47581 ttggagaacc aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag
    47641 atggtgatgt accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt
    47701 tagtccaagg tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag
    47761 agatccgtca agcagaaaat gtgatcctat tcatagatga agttcacgaa atagtcggtg
    47821 ctggttctgc tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc
    47881 gaggagaatt gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag
    47941 acgctgcttt agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa
    48001 cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata
    48061 ctgatgaggc catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct
    48121 tgccggataa agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc
    48181 aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt
    48241 tagcttctcg agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac
    48301 ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag
    48361 atatggaaaa aatcgttgaa caacggacag gtattccagt gggtgaatta aaagaaaaag
    48421 aacagacaca actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata
    48481 acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga
    48541 atcgtccaat cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg
    48601 cgaaacaact tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt
    48661 ctgaatatat ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg
    48721 gatacgaaga agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct
    48781 tgttagatga agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag
    48841 atgatggtcg attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca
    48901 ttatgacaag taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac
    48961 gcgaaggtgt tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc
    49021 tgaaccgttt tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa
    49081 tcgtcacact aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg
    49141 aagttcctac gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg
    49201 ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc
    49261 ttgatcaccc ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg
    49321 ttacttctaa accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc
    49381 agttttatag cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa
    49441 agtcaaaata ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa
    49501 aatacataaa agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc
    49561 ttgtattaaa ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa
    49621 ataccactat tgtctacact gaagcccctg aacataatga aattctgctg atcaatgaca
    49681 aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca
    49741 aagacgccgt ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa
    49801 tctcgattcc aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata
    49861 ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DACEJZ010000009        49600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEJZ010000009 DACEJZ010000000
VERSION     DACEJZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.009"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            151..2316
                     /gene="acm"
                     /locus_tag="IW656_000505"
     CDS             151..2316
                     /gene="acm"
                     /locus_tag="IW656_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0836233.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2588..3073)
                     /locus_tag="IW656_000506"
     CDS             complement(2588..3073)
                     /locus_tag="IW656_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0836234.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3553..4194
                     /locus_tag="IW656_000507"
     CDS             3553..4194
                     /locus_tag="IW656_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0836235.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4213..4848
                     /locus_tag="IW656_000508"
     CDS             4213..4848
                     /locus_tag="IW656_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836236.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4866..5681
                     /locus_tag="IW656_000509"
     CDS             4866..5681
                     /locus_tag="IW656_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0836237.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6000..7436
                     /locus_tag="IW656_000510"
     CDS             6000..7436
                     /locus_tag="IW656_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0836238.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7625..10072
                     /locus_tag="IW656_000511"
     CDS             7625..10072
                     /locus_tag="IW656_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0836239.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10302..11369
                     /gene="rlmN"
                     /locus_tag="IW656_000512"
     CDS             10302..11369
                     /gene="rlmN"
                     /locus_tag="IW656_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0836240.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11562..12665)
                     /locus_tag="IW656_000513"
     CDS             complement(11562..12665)
                     /locus_tag="IW656_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0836241.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13218..13766
                     /gene="aac(6')"
                     /locus_tag="IW656_000514"
     CDS             13218..13766
                     /gene="aac(6')"
                     /locus_tag="IW656_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0836242.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14312..15973
                     /locus_tag="IW656_000515"
     CDS             14312..15973
                     /locus_tag="IW656_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0836243.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16043..16390)
                     /locus_tag="IW656_000516"
     CDS             complement(16043..16390)
                     /locus_tag="IW656_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0836244.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16474..17415
                     /locus_tag="IW656_000517"
     CDS             16474..17415
                     /locus_tag="IW656_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836245.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17416..18459
                     /locus_tag="IW656_000518"
     CDS             17416..18459
                     /locus_tag="IW656_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836246.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18465..19535
                     /locus_tag="IW656_000519"
     CDS             18465..19535
                     /locus_tag="IW656_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836247.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19525..20478
                     /locus_tag="IW656_000520"
     CDS             19525..20478
                     /locus_tag="IW656_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0836248.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20490..21500
                     /locus_tag="IW656_000521"
     CDS             20490..21500
                     /locus_tag="IW656_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0836249.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21785..22606)
                     /locus_tag="IW656_000522"
     CDS             complement(21785..22606)
                     /locus_tag="IW656_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0836250.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22703..22981)
                     /locus_tag="IW656_000523"
     CDS             complement(22703..22981)
                     /locus_tag="IW656_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836251.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23249..25258
                     /gene="metG"
                     /locus_tag="IW656_000524"
     CDS             23249..25258
                     /gene="metG"
                     /locus_tag="IW656_000524"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0836252.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25737..26324)
                     /locus_tag="IW656_000525"
     CDS             complement(25737..26324)
                     /locus_tag="IW656_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836253.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26499..27821
                     /locus_tag="IW656_000526"
     CDS             26499..27821
                     /locus_tag="IW656_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0836254.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27976..28752
                     /locus_tag="IW656_000527"
     CDS             27976..28752
                     /locus_tag="IW656_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0836255.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28745..29314
                     /gene="rnmV"
                     /locus_tag="IW656_000528"
     CDS             28745..29314
                     /gene="rnmV"
                     /locus_tag="IW656_000528"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0836256.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29307..30191
                     /gene="rsmA"
                     /locus_tag="IW656_000529"
     CDS             29307..30191
                     /gene="rsmA"
                     /locus_tag="IW656_000529"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0836257.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30642..31271
                     /locus_tag="IW656_000530"
     CDS             30642..31271
                     /locus_tag="IW656_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836258.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31395..32504)
                     /gene="ugpC"
                     /locus_tag="IW656_000531"
     CDS             complement(31395..32504)
                     /gene="ugpC"
                     /locus_tag="IW656_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0836259.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32713..33135
                     /gene="mgsA"
                     /locus_tag="IW656_000532"
     CDS             32713..33135
                     /gene="mgsA"
                     /locus_tag="IW656_000532"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0836260.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33458..33994
                     /locus_tag="IW656_000533"
     CDS             33458..33994
                     /locus_tag="IW656_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0836261.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(34034..35797)
                     /locus_tag="IW656_000534"
     CDS             complement(34034..35797)
                     /locus_tag="IW656_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836262.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35800..37515)
                     /locus_tag="IW656_000535"
     CDS             complement(35800..37515)
                     /locus_tag="IW656_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836263.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38294..38518)
                     /locus_tag="IW656_000536"
     CDS             complement(38294..38518)
                     /locus_tag="IW656_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836264.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38718..41486
                     /locus_tag="IW656_000537"
     CDS             38718..41486
                     /locus_tag="IW656_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0836265.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            41567..41986
                     /locus_tag="IW656_000538"
     CDS             41567..41986
                     /locus_tag="IW656_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0836266.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41998..42468
                     /locus_tag="IW656_000539"
     CDS             41998..42468
                     /locus_tag="IW656_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0836267.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42485..43264
                     /locus_tag="IW656_000540"
     CDS             42485..43264
                     /locus_tag="IW656_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0836268.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43251..44072
                     /locus_tag="IW656_000541"
     CDS             43251..44072
                     /locus_tag="IW656_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0836269.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44089..45138
                     /locus_tag="IW656_000542"
     CDS             44089..45138
                     /locus_tag="IW656_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0836270.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45151..46155
                     /locus_tag="IW656_000543"
     CDS             45151..46155
                     /locus_tag="IW656_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0836271.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46788..47807
                     /locus_tag="IW656_000544"
     CDS             46788..47807
                     /locus_tag="IW656_000544"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0836272.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            47850..48731
                     /gene="galU"
                     /locus_tag="IW656_000545"
     CDS             47850..48731
                     /gene="galU"
                     /locus_tag="IW656_000545"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0836273.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            49031..49408
                     /locus_tag="IW656_000546"
     CDS             49031..49408
                     /locus_tag="IW656_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836274.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT        16459 a         8565 c        10261 g        14315 t
ORIGIN      
        1 ggcatattta ttttttataa atatttgtaa aataactatc aattttttag ttaaaagatt
       61 ggaaatgagt gggggaggaa ttgaagaaag gatttttgag agatgatata gtaggtttta
      121 tttttagtta aaatagggga gatagaaaaa atgaaaaagt gtagaaatat tattttatca
      181 atgttattta ttattactaa tataacatca ctgattcctg ttcatgtcta tgcggatgca
      241 ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg acccaacgaa tattacagat
      301 ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt
      361 gattatcttt ggatcaattg gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa
      421 gaaatcccat taatgattga aaataaaaat gttggaactt taacggtcag aaaagattct
      481 gcgcaggttg tgtttaatga aaatattaaa aacctagact cagtagaagg ttgggggcaa
      541 tttgagatac aggccagaaa cgtaaccgat accggtgaag aaaatacggg accattcacc
      601 gtgacgagcg gtgataaaac agctactgta aatgtgacaa agccagcttc tggttcttct
      661 tctagcgttt tttattataa aacaggagac atgctacctg aagatactaa gcacattcga
      721 tggtttttga atattaacaa taacggcact tatgttgaac aaccagttaa aatcagtgat
      781 gaaatccaaa gtggacaaag attagatcca agtacgtttg aaattaatca aatacactta
      841 ggtgaacaga aggtttatag aggagaagag ggaatccaac aatttttaca agatttcccc
      901 agcgctacat ttaatttttc agtaactgat aattacattg aaataactat accaaaaaac
      961 tttgtcaatc ttaggaaaat aatggtgagt tataagacga tcatagaaaa cccagaacaa
     1021 ataaattttg agaatcattc ggaagcttgg tttaaagaat ttaataaacc tgctgttgat
     1081 ggagaaagtt tcaatcatac agttaaaaat atttcagctt ctggtggagt caacggtaca
     1141 gttagaggcg aattgaagat tttcaaatat atcaatgaca cagagattgg tatcccaaat
     1201 gtgactttcg aattaagacg agcagatgaa cagcctattc aaggacagtc tagtatcctg
     1261 ttgacttcga atgaacaggg agaagctagt atcaaaggcc tgcaagtagg agactatgtt
     1321 gtaaaagaaa aagaagcacc caattggatt gattttgatc cactaagttc aaatgaactc
     1381 aaattctcca taaacgaaaa tgatacagag ggagtcagtt tacctattta taacaagaaa
     1441 aaggttacga acatcacagc aactaaaatt tggaacggtg gaaccactcc aagaccgagt
     1501 atttatttta aattattcag agcaagtaca aacaattggg aaccagttcc tgatgcagaa
     1561 acaaaacgat tagacaatgg aataacttca gtgacttggg aagatatcca acagtatgat
     1621 gatagtggaa acgaatatac gtttaaagtg caagaagtag atcaaaacgg aaatgattat
     1681 gtcccttcag gttatcaaaa aattgaaaac gggctagtcg ttacaaatga gaataaagaa
     1741 gtcatttcag tctcaggaca aaaaacatgg gaagacaatg agaaccaaga cggcaaacga
     1801 ccgacgacga tcacagtaaa tctattagcc gacggtcaac cgatccaaca caaagaagta
     1861 agtgaaaaag atgattggcg ctatcgtttc acaaatctgc caaaatacag agacgggcaa
     1921 gaaatcatct atactgtaac ggaagacaac gtgcccgaat acagtacgac gattgacggg
     1981 tataacatca aaaacagtta tacaccggaa aaaacaaata tatctatacc aggtagccat
     2041 ataacaccgt ggaaaaattt tccagagaag acagagagag agaaaattag aaatctattc
     2101 tctcaaaaat tgcaaatatt tgctcataca ggagaatcca gcgaacaaag aagcaattat
     2161 attagtaaga gagctgtacc taaacagata acaaataaat caaaaagcat tcttcctaaa
     2221 acaagtagta aaaaatctag ttttgctgta atcattggtt tattgatagt aactatctgt
     2281 gctgggatat tcttacaaaa atataaaaaa gtttagttag aatataatat tttttgttaa
     2341 atatgactga aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta
     2401 tatatgcttc ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac
     2461 tcgtattagt tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt
     2521 ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac
     2581 tctatcatta caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa
     2641 agtcggaaaa atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat
     2701 cttgacgatc aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca
     2761 atgctaatgg agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag
     2821 aaacaaaaga taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct
     2881 tttcatcagg caagatgaca agatagattt gttttccttt tcttgatttc aataatagat
     2941 tttttacttg cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat
     3001 gatcgacttt ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg
     3061 ttgcacgact catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg
     3121 acaaatgaaa aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga
     3181 agagtagccg ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac
     3241 cgtgaaacac atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc
     3301 gttacagctg cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg
     3361 taaaaaaagg tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg
     3421 ttttcatctg aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag
     3481 gtggaaccac gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag
     3541 gaggaaaaga agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc
     3601 acaacattga aattattttt atttaccttg attggctcat taccattagg gattgtttta
     3661 gcattaggat tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg
     3721 gttatgcggg gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg
     3781 attgggatcg tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca
     3841 gcttattttg cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa
     3901 gcagcgcaag ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa
     3961 gtgacaaaga ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct
     4021 tccttgatct atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa
     4081 cgcgacgcga ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt
     4141 attctaacaa tcataatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata
     4201 ggaggaagaa atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata
     4261 atcaaagatt tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc
     4321 ggtggcggta aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga
     4381 gaattttatt tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt
     4441 gtaggagtcg tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt
     4501 accttagcac caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa
     4561 gaattagcgg cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct
     4621 ggcggacaaa aacagcgggt agcgattgcc agagcattgg ctatgaatcc acgggtattg
     4681 gcttatgatg aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg
     4741 atcctatcat tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt
     4801 gcagaaaatg tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga
     4861 tagagatgaa aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag
     4921 gctgttcgaa caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa
     4981 tcgttatagg tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc
     5041 tcacaggatt tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag
     5101 actttcagcc cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt
     5161 tgatatggaa cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac
     5221 cttatatgga aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg
     5281 gacaaatgaa taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta
     5341 cgaaacaacc agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata
     5401 gttttaatga agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata
     5461 aagtatacgc agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa
     5521 aaggttctta tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag
     5581 tgaaaaaaat caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat
     5641 ccgataaatg gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca
     5701 aaagacgtgt cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga
     5761 ctcggagctg aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt
     5821 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
     5881 tactcgaaac taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa
     5941 ggggtttcgt gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa
     6001 tggaacacaa agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag
     6061 cgtatcaagt agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat
     6121 ttgtacggat acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact
     6181 atcatcgtta taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct
     6241 tctctgtagc ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc
     6301 tcgcttttta tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga
     6361 ctttatatca ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac
     6421 gtcagatcat tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga
     6481 aagtccatta ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt
     6541 tagcagcaca tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg
     6601 ctaatttagc aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa
     6661 tcggaccaag ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc
     6721 tggctgctga aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg
     6781 gaaaatatcc gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg
     6841 aagctggcga tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact
     6901 atcaaacggc gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata
     6961 cgacaggaaa aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg
     7021 agaatccatt tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga
     7081 tcggactgcg tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct
     7141 taggagagta tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt
     7201 acttacaaag ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag
     7261 gctattgcac ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat
     7321 atggatttgt atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga
     7381 aagacagtta ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca
     7441 catacttaaa acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt
     7501 tagaaattaa gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca
     7561 aacaaaactt tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata
     7621 ataaatggca caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa
     7681 gcaaaaacag cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt
     7741 tgctgtcttc ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg
     7801 gatcattgca ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg
     7861 gattgtcatt aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat
     7921 actcttttat ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac
     7981 tggggaacca aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag
     8041 tcaagtagac aataacgttg gtggtgggat gattggagca atcctttatc attttactta
     8101 cttcttgatt gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc
     8161 attcctgttc agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga
     8221 acgagtacag gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa
     8281 ggaagagaga atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga
     8341 agctgcagaa aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga
     8401 aaatactgct gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt
     8461 tccaatcgac agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga
     8521 tacaccaaaa cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg
     8581 tacctcatta gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac
     8641 agttgatttg cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga
     8701 aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt
     8761 caaagccagc ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa
     8821 agtaagtaaa attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt
     8881 ccggatggaa gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa
     8941 gatcagtatg gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt
     9001 actagaagta cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa
     9061 gatgcctcac ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg
     9121 gattatcaca agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga
     9181 tccgaaaatg gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt
     9241 gacgaaccct agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg
     9301 atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca
     9361 acagaaaaac ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt
     9421 agatgaatta gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg
     9481 tttagcgcaa atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag
     9541 tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt
     9601 atcaagcggt acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg
     9661 gcgaggagat atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc
     9721 attcatttct gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc
     9781 tcattatgaa gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca
     9841 gccacaagat gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag
     9901 tatttccctt ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga
     9961 tgaactggaa gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt
    10021 cattgagcag gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg
    10081 tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga
    10141 aaaaatggtt tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg
    10201 tgagattaaa gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt
    10261 atagtatgta gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt
    10321 atggcttgac gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc
    10381 gagctgccca agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga
    10441 cgaatctatc gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa
    10501 aacagatggt cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg
    10561 ataaaaacat gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga
    10621 cgacacaagt aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga
    10681 accgtgatct gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg
    10741 atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat
    10801 ttgataatta cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg
    10861 caattggtgc tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat
    10921 tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg
    10981 tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg
    11041 tggatgagta tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc
    11101 aagtaaatga tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa
    11161 aattagcata tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc
    11221 gcagtccaaa agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact
    11281 gtgtgatacg taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca
    11341 agcaaatgaa aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata
    11401 aaaggagcag ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata
    11461 gcgaaagcta taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc
    11521 aatttgatga cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat
    11581 ccttgctggc tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat
    11641 ttttctcggg tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct
    11701 aagattgctt ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt
    11761 tggttctttt tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc
    11821 aataaggtca gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct
    11881 agaacagtcg taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact
    11941 aaaacagaaa ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat
    12001 gagggacgat cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca
    12061 atgcacattt ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga
    12121 gcagaaaaag tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat
    12181 ttatcaggtg cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa
    12241 ggaaagaaga gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg
    12301 ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca
    12361 atgactttta cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt
    12421 tggacacttt catgctggat caactgttca atatttccat aagttgaatt acccccgtac
    12481 cagagactgg cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc
    12541 tttgaaagtg cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt
    12601 ccatttgctt tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga
    12661 ctcatattca tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat
    12721 ctgactgcgg ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg
    12781 tggcatgaac accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat
    12841 tgaaaacgac aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt
    12901 tttgtaatta tctaaaaatt ctaaaaatta agattattat atgaaaacag gttttatttg
    12961 tcaattcata attggttttt tttaaagcaa aagataatct atatcatgat gaacaaacag
    13021 agttttttcc tccaattgat ggctttcctg atctttccta ttttgaataa agtgtttctg
    13081 tataattttt ttattttata agtaagataa ataatgaaag aatagagata ctgattggta
    13141 gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag
    13201 aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag
    13261 atcagctttc tgatttactg agactgactt ggccggaaga atatggagac agctcggcag
    13321 aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg
    13381 agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc
    13441 cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact
    13501 tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag
    13561 accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt
    13621 ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg
    13681 taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga
    13741 ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta atcagcttca
    13801 agaaataaga aggaatttac gaaaattact tcttgaattt ttgtgaaatt ccaacttatt
    13861 tccgaagaag ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt cgcttagttt
    13921 cgagtagtaa gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa atttggctta
    13981 ataccgagaa actggctttt gaacaccgtt tattcagata agggactgtg acaagacttt
    14041 tgtcacagtt tctttttgtt tattcttttt tatctttgta tggcaataat agacaatata
    14101 acgaaaatta tttaatggct tttggaagac catggaaaac cttgtacaaa gctatttttt
    14161 ctgaaaaatt ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa
    14221 gtgttgattt tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta
    14281 ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt
    14341 tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag
    14401 ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga
    14461 aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa
    14521 cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag
    14581 cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc
    14641 aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac
    14701 agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga
    14761 agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta
    14821 tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc
    14881 atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc
    14941 aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac
    15001 agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact
    15061 ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga
    15121 tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc
    15181 agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa
    15241 gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga
    15301 agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc
    15361 agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt
    15421 ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt
    15481 tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga
    15541 atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt
    15601 tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg
    15661 atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc
    15721 atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc
    15781 aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat
    15841 ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga
    15901 aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata
    15961 cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc
    16021 gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa
    16081 gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg
    16141 agagcgagga aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc
    16201 gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa
    16261 acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga
    16321 tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt
    16381 tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat
    16441 ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta
    16501 aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg
    16561 atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc
    16621 gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat
    16681 ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc
    16741 gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc
    16801 tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg
    16861 gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt
    16921 gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg
    16981 gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac
    17041 tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta
    17101 gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt
    17161 ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg
    17221 gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca
    17281 acaaatgatt atcctacgat catggctgta acgatccttt actcatttat gctgattttt
    17341 gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa
    17401 gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct
    17461 gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca
    17521 ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt
    17581 atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct
    17641 cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga
    17701 atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat
    17761 gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt
    17821 gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct
    17881 gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga
    17941 cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt
    18001 gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg
    18061 gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa
    18121 gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc
    18181 aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca
    18241 tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca
    18301 gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta
    18361 ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat
    18421 ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag
    18481 aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg
    18541 gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg
    18601 gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg
    18661 ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa
    18721 aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga
    18781 cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa
    18841 aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga
    18901 aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg
    18961 cgattgcact ggtatgttat ccggaaatcc tgattgctga tgaacctaca acagcgcttg
    19021 atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata
    19081 catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg
    19141 ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc
    19201 cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg
    19261 atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg
    19321 cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt
    19381 ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag
    19441 tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata
    19501 cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa
    19561 cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac
    19621 atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact
    19681 ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa
    19741 gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg
    19801 atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc
    19861 gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat
    19921 gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt
    19981 tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt
    20041 atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat
    20101 ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg
    20161 tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag
    20221 atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta
    20281 tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa
    20341 cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc
    20401 tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct
    20461 cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc
    20521 cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata
    20581 accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc
    20641 tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt
    20701 tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca
    20761 gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa
    20821 cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca
    20881 agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat
    20941 ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt
    21001 tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc
    21061 tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga
    21121 aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca
    21181 attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag
    21241 aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag
    21301 aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa
    21361 taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg
    21421 acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac
    21481 attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg
    21541 ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac
    21601 taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat
    21661 tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa
    21721 ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac
    21781 aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta
    21841 caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat
    21901 cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg
    21961 tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac
    22021 ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag
    22081 actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt
    22141 tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg
    22201 gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat
    22261 taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt
    22321 cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac
    22381 caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca
    22441 gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt
    22501 catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgctttat
    22561 gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat
    22621 tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta
    22681 cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata
    22741 aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc
    22801 cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac
    22861 tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata
    22921 ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca
    22981 tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga
    23041 aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct
    23101 attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca
    23161 ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg
    23221 catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac
    23281 gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg
    23341 tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt
    23401 ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga
    23461 atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta
    23521 tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga
    23581 tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc
    23641 tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa
    23701 cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata
    23761 cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga
    23821 attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt
    23881 agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc
    23941 gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata
    24001 tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg
    24061 taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt
    24121 gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc
    24181 taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc
    24241 actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga
    24301 agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg
    24361 aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt
    24421 cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga
    24481 tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg
    24541 tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa
    24601 agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt
    24661 acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc
    24721 tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt
    24781 tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca
    24841 agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga
    24901 aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga
    24961 actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca
    25021 attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt
    25081 ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc
    25141 acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg
    25201 ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa
    25261 attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca
    25321 attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa acagagaact
    25381 atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat
    25441 aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt
    25501 ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa
    25561 tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc
    25621 tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa
    25681 aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac
    25741 ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc
    25801 agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga
    25861 tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata
    25921 aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa
    25981 taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat
    26041 aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa
    26101 tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac
    26161 aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt
    26221 aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg
    26281 gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc
    26341 gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg
    26401 agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg
    26461 aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag
    26521 aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct
    26581 tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt
    26641 ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag
    26701 caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca
    26761 gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat
    26821 cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt
    26881 tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg
    26941 gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca
    27001 tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa
    27061 aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg
    27121 acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc
    27181 ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg
    27241 tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag
    27301 catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct
    27361 cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg
    27421 ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg
    27481 ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt
    27541 ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt
    27601 ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac
    27661 cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga
    27721 cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc
    27781 cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca
    27841 gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt
    27901 tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga
    27961 aaaggagaga taaacatgat atttgattct catacccatt taaatgctga acagttcaat
    28021 gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc
    28081 gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat
    28141 atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa
    28201 aataaattac aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg
    28261 gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt
    28321 gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac
    28381 acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc
    28441 agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc
    28501 ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc
    28561 gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag
    28621 cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt
    28681 ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc
    28741 gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag
    28801 aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga
    28861 agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga
    28921 tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa
    28981 acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga
    29041 gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc
    29101 ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg
    29161 ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa
    29221 cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca
    29281 agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta
    29341 gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt
    29401 ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata
    29461 cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg
    29521 caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga
    29581 tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa
    29641 cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt
    29701 actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac
    29761 tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag
    29821 cttatggttc attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg
    29881 taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa
    29941 gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag
    30001 cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag
    30061 atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac
    30121 gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa
    30181 atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta
    30241 ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg
    30301 cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta
    30361 agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt
    30421 acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt
    30481 cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt
    30541 ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa
    30601 gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc
    30661 gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc
    30721 ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg
    30781 cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct
    30841 ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga
    30901 ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt
    30961 ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag
    31021 aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc
    31081 aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag
    31141 ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg
    31201 tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa
    31261 cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc
    31321 ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc
    31381 taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg
    31441 cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca
    31501 tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca
    31561 agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct
    31621 acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa
    31681 cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca
    31741 tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata
    31801 aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt
    31861 tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga
    31921 tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca
    31981 cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact
    32041 tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa
    32101 gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca
    32161 cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca
    32221 aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct
    32281 ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct
    32341 tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa
    32401 acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca
    32461 ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt
    32521 tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt
    32581 catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa
    32641 tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa
    32701 ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg
    32761 aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg
    32821 actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg
    32881 ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt
    32941 tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt
    33001 ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta
    33061 agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat
    33121 ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt
    33181 agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat
    33241 aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt
    33301 gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg
    33361 ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca
    33421 tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc
    33481 acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt
    33541 agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg
    33601 cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat
    33661 ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg
    33721 agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc
    33781 tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat
    33841 gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat
    33901 atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa
    33961 aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga
    34021 agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat
    34081 ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta
    34141 atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc
    34201 cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc
    34261 tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca
    34321 acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt
    34381 tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca tcattgattt
    34441 ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata
    34501 gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc
    34561 ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca
    34621 aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg
    34681 gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa
    34741 catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca
    34801 aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag
    34861 cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg
    34921 agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca
    34981 ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg
    35041 ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta
    35101 gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt
    35161 taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga
    35221 aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac
    35281 aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga
    35341 tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt
    35401 ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa
    35461 gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag
    35521 ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac
    35581 caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca
    35641 tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga
    35701 ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc
    35761 ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc
    35821 gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc
    35881 aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg
    35941 ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc
    36001 tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat
    36061 caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc
    36121 catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat
    36181 tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc
    36241 acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag
    36301 atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac
    36361 atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat
    36421 gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga
    36481 atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc
    36541 gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac
    36601 ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag
    36661 tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct
    36721 accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat
    36781 caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc
    36841 tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg
    36901 gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc
    36961 ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa
    37021 aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac
    37081 cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc
    37141 tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg
    37201 attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt
    37261 accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc
    37321 aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat
    37381 ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc
    37441 ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg
    37501 taataactga atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt
    37561 actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg
    37621 aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa
    37681 aatgagaaaa catgccagtc tcttcattca taggaacgtc tattttactt aatttttcta
    37741 taaatgaatt ctgcataaaa aatatatcaa gtaaatgttt atagatgaaa ctctatttac
    37801 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
    37861 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
    37921 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
    37981 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
    38041 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
    38101 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
    38161 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
    38221 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
    38281 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
    38341 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
    38401 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
    38461 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
    38521 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
    38581 actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga
    38641 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
    38701 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
    38761 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
    38821 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
    38881 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
    38941 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
    39001 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
    39061 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
    39121 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
    39181 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
    39241 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
    39301 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
    39361 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
    39421 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
    39481 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
    39541 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
    39601 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
    39661 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
    39721 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
    39781 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
    39841 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
    39901 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
    39961 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
    40021 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
    40081 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
    40141 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
    40201 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
    40261 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
    40321 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
    40381 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
    40441 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
    40501 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
    40561 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
    40621 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
    40681 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
    40741 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
    40801 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
    40861 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
    40921 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
    40981 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
    41041 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
    41101 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
    41161 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
    41221 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
    41281 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
    41341 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
    41401 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
    41461 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
    41521 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
    41581 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
    41641 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
    41701 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
    41761 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
    41821 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
    41881 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
    41941 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
    42001 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
    42061 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
    42121 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
    42181 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
    42241 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
    42301 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
    42361 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
    42421 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
    42481 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
    42541 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
    42601 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
    42661 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
    42721 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
    42781 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
    42841 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
    42901 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
    42961 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
    43021 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
    43081 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
    43141 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
    43201 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
    43261 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
    43321 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
    43381 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
    43441 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
    43501 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
    43561 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
    43621 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
    43681 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
    43741 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
    43801 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
    43861 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
    43921 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
    43981 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
    44041 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
    44101 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
    44161 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
    44221 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
    44281 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
    44341 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
    44401 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
    44461 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
    44521 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
    44581 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
    44641 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
    44701 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
    44761 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
    44821 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
    44881 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
    44941 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
    45001 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
    45061 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
    45121 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
    45181 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
    45241 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
    45301 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
    45361 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
    45421 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
    45481 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
    45541 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
    45601 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
    45661 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
    45721 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
    45781 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
    45841 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
    45901 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
    45961 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
    46021 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
    46081 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
    46141 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
    46201 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
    46261 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
    46321 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
    46381 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
    46441 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
    46501 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
    46561 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
    46621 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
    46681 acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa
    46741 tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg aaacaaaaaa
    46801 tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg gcagaaaacg
    46861 ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc aataagtatc
    46921 atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc ggatataaag
    46981 aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca acaaaagcga
    47041 ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc attatacatg
    47101 caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg gctgaagaaa
    47161 ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat gctgaggaag
    47221 tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa gcagcagcct
    47281 atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat gatgtgattg
    47341 gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc gctatccatg
    47401 gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg gcagaaatca
    47461 gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg
    47521 gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt
    47581 taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc gttgagggtg
    47641 tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag atgccaatta
    47701 ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca gcaaaagaaa
    47761 tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata gatgatcaat
    47821 agttaatcgt cgtttaggag gaacagcaag tgaaggtcaa aaaagcagtc attcctgccg
    47881 ctgggctagg aacacgtttt ttgccggcaa caaaagccat ggcaaaagaa atgcttccaa
    47941 tcgtggataa accaacgatc cagtttattg tggaagaagc attggattcg ggaatcgaag
    48001 acatcttgat cgtaacggga aaagaaaaaa gacccattga agatcacttt gatgcaaata
    48061 tcgaattgga aaacaatttg cgggaaaagg aaaaaacaga attactcgct ttagttgaag
    48121 aaacgactca agtcaatctt cacttcatcc gtcaatctca tccaagagga ctgggggatg
    48181 ccgtgcttca agcaaaagca tttataggaa atgaaccgtt tgtcgtcatg ctgggggatg
    48241 atttgatgaa agatgatatc ccattgactc aacaattgat gatggattat gaagaaacac
    48301 aagcttccgc tgttgccgtc atgcgtgtac cagaaaatga aacatctaaa tacggaatta
    48361 ttgatcctaa aacagatagc gaaaagggtc gttgcagagt aaaagggttt gtagaaaaac
    48421 ctgaaatggg aaaagcacca agtaatttag ccattatcgg acgttatcta ctgactccta
    48481 aaatatttga gatattagaa actcaagaac caggagcagg aaacgagata cagctaacgg
    48541 atgcacttca gactcttaac cagacagagg cggtctatgc gagagagttt aaaggaaaaa
    48601 gatacgacgt aggcgataag ttaggatata tgaagacaaa tattgaatat ggacttcaac
    48661 attcggaaat tgggacttca ttaagtgcat atattatcca gttgagtaaa aagctaagaa
    48721 cagaaagtta actggaaata agtggcctgc tgagaatgaa aggctatttg ttattaaagc
    48781 ggcagcttgt gcgtcatttg cgcaactgtc gttttagttt tttgactcta gatcagttga
    48841 gtcgtggaaa acgaaagata gaaaaagact ttcgtgaata gattcagaaa atattatgct
    48901 aatggtttaa ttttttttta agttgtataa aagaaaatga ttacataaga gaaatgaaag
    48961 ccaagccaga agattcatag tagaataaaa aggcgaattt tttaattata aaaaaaggtg
    49021 tgtgttcact atgggaaaat tagtcgctgt tgcacagtcg ctgcttgata tactcaagtg
    49081 gctgatggaa ggaagcaatt ggatatttgt gttgccacta ttgcttatta gtctatttat
    49141 catgtttcgc atggtcagaa aaaaagaaac gtttctgatt tttatctggt atgcagcatc
    49201 cgttggggtt gctaccttga tggttgaatt ggcgttaggt agttgggaag cttgcgtatt
    49261 tgctttattt tttgctgtac tgtatctaat catttatatc ccatatatga aaagaaagtc
    49321 tcggataggc agaaaaggta gacatacaac tactttttct agtacagatt ctctcgaaca
    49381 aagtagaaga acgagaagaa gacggtaata tttagtaggt atacgcaaaa aaagataaaa
    49441 taatggataa actattgaga tgagagattt atttaattgg tggaaaaaag cttttttcac
    49501 ttatataaaa gaatcttttt taaaggaatg cttgttgtat cgggataagc cattcctttt
    49561 tttatgttat ttatatatat ataaatcata ccaatgtttt
//
LOCUS       DACEJZ010000010        43647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEJZ010000010 DACEJZ010000000
VERSION     DACEJZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.010"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(123..647)
                     /locus_tag="IW656_000547"
     CDS             complement(123..647)
                     /locus_tag="IW656_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0836275.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW656_000548"
     CDS             complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW656_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0836276.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW656_000549"
     CDS             complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW656_000549"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0836277.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2369..3049)
                     /locus_tag="IW656_000550"
     CDS             complement(2369..3049)
                     /locus_tag="IW656_000550"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0836278.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3175..4044
                     /locus_tag="IW656_000551"
     CDS             3175..4044
                     /locus_tag="IW656_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0836279.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4070..4585
                     /locus_tag="IW656_000552"
     CDS             4070..4585
                     /locus_tag="IW656_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836280.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4632..5195)
                     /locus_tag="IW656_000553"
     CDS             complement(4632..5195)
                     /locus_tag="IW656_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0836281.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5250..5429)
                     /locus_tag="IW656_000554"
     CDS             complement(5250..5429)
                     /locus_tag="IW656_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836282.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5506..6015)
                     /locus_tag="IW656_000555"
     CDS             complement(5506..6015)
                     /locus_tag="IW656_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836283.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6111..6491)
                     /locus_tag="IW656_000556"
     CDS             complement(6111..6491)
                     /locus_tag="IW656_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0836284.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6820..7569
                     /locus_tag="IW656_000557"
     CDS             6820..7569
                     /locus_tag="IW656_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0836285.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7753..9030)
                     /locus_tag="IW656_000558"
     CDS             complement(7753..9030)
                     /locus_tag="IW656_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836286.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9109..9753)
                     /gene="pcp"
                     /locus_tag="IW656_000559"
     CDS             complement(9109..9753)
                     /gene="pcp"
                     /locus_tag="IW656_000559"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0836287.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9952..10896)
                     /locus_tag="IW656_000560"
     CDS             complement(9952..10896)
                     /locus_tag="IW656_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0836288.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10898..11584)
                     /locus_tag="IW656_000561"
     CDS             complement(10898..11584)
                     /locus_tag="IW656_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0836289.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12058..12378
                     /locus_tag="IW656_000562"
     CDS             12058..12378
                     /locus_tag="IW656_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0836290.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12532..13437
                     /locus_tag="IW656_000563"
     CDS             12532..13437
                     /locus_tag="IW656_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0836291.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13434..14207
                     /locus_tag="IW656_000564"
     CDS             13434..14207
                     /locus_tag="IW656_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0836292.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14388..15812)
                     /locus_tag="IW656_000565"
     CDS             complement(14388..15812)
                     /locus_tag="IW656_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0836293.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16023..16469
                     /gene="nrdI"
                     /locus_tag="IW656_000566"
     CDS             16023..16469
                     /gene="nrdI"
                     /locus_tag="IW656_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0836294.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16534..18516)
                     /locus_tag="IW656_000567"
     CDS             complement(16534..18516)
                     /locus_tag="IW656_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0836295.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18530..19426)
                     /gene="murQ"
                     /locus_tag="IW656_000568"
     CDS             complement(18530..19426)
                     /gene="murQ"
                     /locus_tag="IW656_000568"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0836296.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19429..20490)
                     /locus_tag="IW656_000569"
     CDS             complement(19429..20490)
                     /locus_tag="IW656_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0836297.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20701..21228)
                     /locus_tag="IW656_000570"
     CDS             complement(20701..21228)
                     /locus_tag="IW656_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0836298.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21295..22797)
                     /locus_tag="IW656_000571"
     CDS             complement(21295..22797)
                     /locus_tag="IW656_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0836299.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23055..24176)
                     /locus_tag="IW656_000572"
     CDS             complement(23055..24176)
                     /locus_tag="IW656_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0836300.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24302..25243
                     /locus_tag="IW656_000573"
     CDS             24302..25243
                     /locus_tag="IW656_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0836301.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25393..26289)
                     /locus_tag="IW656_000574"
     CDS             complement(25393..26289)
                     /locus_tag="IW656_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0836302.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26558..27898
                     /locus_tag="IW656_000575"
     CDS             26558..27898
                     /locus_tag="IW656_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0836303.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28116..28559
                     /locus_tag="IW656_000576"
     CDS             28116..28559
                     /locus_tag="IW656_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0836304.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29063..30481)
                     /gene="nhaC"
                     /locus_tag="IW656_000577"
     CDS             complement(29063..30481)
                     /gene="nhaC"
                     /locus_tag="IW656_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0836305.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30884..32107)
                     /locus_tag="IW656_000578"
     CDS             complement(30884..32107)
                     /locus_tag="IW656_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0836306.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32138..32977)
                     /locus_tag="IW656_000579"
     CDS             complement(32138..32977)
                     /locus_tag="IW656_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0836307.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32994..33674)
                     /locus_tag="IW656_000580"
     CDS             complement(32994..33674)
                     /locus_tag="IW656_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836308.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33676..34749)
                     /locus_tag="IW656_000581"
     CDS             complement(33676..34749)
                     /locus_tag="IW656_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0836309.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35396..36067)
                     /locus_tag="IW656_000582"
     CDS             complement(35396..36067)
                     /locus_tag="IW656_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0836310.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36154..38832)
                     /gene="mgtA"
                     /locus_tag="IW656_000583"
     CDS             complement(36154..38832)
                     /gene="mgtA"
                     /locus_tag="IW656_000583"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0836311.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39491..39928
                     /gene="ndk"
                     /locus_tag="IW656_000584"
     CDS             39491..39928
                     /gene="ndk"
                     /locus_tag="IW656_000584"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0836312.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39950..40816)
                     /locus_tag="IW656_000585"
     CDS             complement(39950..40816)
                     /locus_tag="IW656_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0836313.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40976..43069)
                     /locus_tag="IW656_000586"
     CDS             complement(40976..43069)
                     /locus_tag="IW656_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0836314.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12606 a         9142 c         7709 g        14190 t
ORIGIN      
        1 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
       61 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
      121 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
      181 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
      241 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
      301 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
      361 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
      421 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
      481 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
      541 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
      601 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
      661 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
      721 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
      781 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
      841 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
      901 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
      961 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     1021 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     1081 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     1141 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     1201 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     1261 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     1321 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     1381 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     1441 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     1501 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     1561 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     1621 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     1681 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     1741 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     1801 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     1861 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     1921 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     1981 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     2041 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     2101 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     2161 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     2221 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     2281 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     2341 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     2401 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     2461 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     2521 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     2581 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     2641 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     2701 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     2761 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     2821 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     2881 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     2941 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     3001 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     3061 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     3121 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     3181 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     3241 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     3301 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     3361 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     3421 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     3481 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     3541 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     3601 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
     3661 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
     3721 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
     3781 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
     3841 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
     3901 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
     3961 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
     4021 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
     4081 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
     4141 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
     4201 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
     4261 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
     4321 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
     4381 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
     4441 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
     4501 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
     4561 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
     4621 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
     4681 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
     4741 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
     4801 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
     4861 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
     4921 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
     4981 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
     5041 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
     5101 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
     5161 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
     5221 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
     5281 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
     5341 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
     5401 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
     5461 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
     5521 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
     5581 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
     5641 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
     5701 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
     5761 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
     5821 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
     5881 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
     5941 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
     6001 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
     6061 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
     6121 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
     6181 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
     6241 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
     6301 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
     6361 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
     6421 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
     6481 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
     6541 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
     6601 ggcaaaaaaa cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg
     6661 aaagtaagaa attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt
     6721 ataattgaaa gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac
     6781 agaacacact acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt
     6841 ttagctcatc agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt
     6901 ggctgccagg tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt
     6961 atcggtacca cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac
     7021 ggtattttag gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac
     7081 tatttaagta tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta
     7141 agtaaagaat ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg
     7201 tttatcgcaa tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt
     7261 tatacagatg atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct
     7321 ggtatcatca gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt
     7381 gctcaaatgg tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc
     7441 gtgaacagct ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat
     7501 aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa
     7561 cacgcataaa aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
     7621 acacgtataa acggcggaat cagaagcaac tccttcggaa ataagctgaa attcacaaaa
     7681 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
     7741 agcctctatt ttttatagta attccttcag atactttctt gcctcatcat tgaaacgcag
     7801 tgattcttct aacttctgat aatattccaa cgcttgtttc tgagcttcag taaaacgata
     7861 atgttttcct ttccatttgt cgattgtctc ttgcacattt ccagattgga tatctttcac
     7921 tactagttgc catttttctt ttgagactgt acggtctttc ttatcagcag attgtatagc
     7981 ttctcgttct ttacgcaata cttttaaaaa accatttctg actatccgag gaatgctgtc
     8041 gaagaaccct tctgtaaaac catagcgaca atattgcttc caaataaata gatagatttt
     8101 tggttggatg aatcgttggt ttttcgcaaa atattccatc tgagcagtga cttctggtgt
     8161 tcgttgacgt tttgtctttc tcaacacgga aataaatgtt tcaaaataag cattaggatg
     8221 tactggcact tcttcatata cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt
     8281 gatcaagaat tcttctacat cctttactaa ttcttctgca aagtcgtcaa atagttcttt
     8341 ttttatctgt cgcttttttg actgcccatt gatgtgttca cttattttct tgatttcatt
     8401 ttccaatgct tgataatccg ctatgtaaat cggcatttct acttggcgaa attctgcgag
     8461 ccgctgagct aattttatat ccggtaaata tggctgtttc atccgacgat tttggtacaa
     8521 gacaaaacag tatttttgcc atagtccttc aggaaaatcg atattcttct gcttaagcca
     8581 taataattcg tctaaggcca gatcaacttt tgtcattccc tgatggatcg aagctgccac
     8641 tgtcggagag acatgcaggt gatcggcaaa gtggttgatg cctagtttta ggatctttcc
     8701 ggtttttggc gatttaacga tgtattgcac ttttaattct ctaccacact cacaatacaa
     8761 tttttgctgg cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca
     8821 atctgttgca gctaaataat gttcttccat aaaacgagag ctgattagat atcggcggtg
     8881 cttatcgact agttcccgct gttttttcgt taattttgca tagatctccc aacgttgact
     8941 gcttgtgtat tgtttagaaa ttttgatggg atagcttttg ctgatttctt ttggaaacca
     9001 ttcttcaaag acggattgcc attcttccac ggctgtttct ttcctttcta attacaaaaa
     9061 gctggctact aacataacgt tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt
     9121 tccggcattt ccggaaatat cttctttatt ttctactgct gcttcaactg cataagtcaa
     9181 agaagaagca atgtcttcta aagacataga aggcgtaccc actggacgat cgatcacttg
     9241 atcgggtgag aatggcacgt ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat
     9301 caaatgaagc aaacgataca tgatatcatt gcagacaaat gttcctgctg tataagagat
     9361 gtacgctggt agaccatgat cttgtacatt ttttgtcatt gctttgattg gcaatgaggt
     9421 aaagtaagcg gctggaccgt tttcttctaa agccgtatcg aatggctgat ttccttcatt
     9481 atcagggatt cttgcttctg ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg
     9541 cccacctgct tgtccgacac agatcacgac atctggttgg tgctgttgga tggcttcttt
     9601 gactacttga ctgctttttt cgaatacggt cggtacttcc actttgatga tttcagcacc
     9661 gctgatttca tctggcattt tttttactgc ttcataggct ggattcactt tgtccccacc
     9721 aaatggatca aaacctgtta ctagtacttt catctgttgt tcctccgtcc gattgattta
     9781 acatatggtt atcttttatc ataccatttt ccttgaacag cgtaaataaa ggaaacaata
     9841 gtttaaataa cataacagat caaacttaat atattcttat aaaaaaatag tgttccacta
     9901 caatgttaga agaggttgcg acaattattc tgtcacaacc cctctcttct attatttaaa
     9961 tagaatcata taagcaattt ggaaaactag cataaataac gcaataaaca actggttttt
    10021 gatcactccg tatttgtctt tcatctccag catcgctaca ggcacgatat tgaagttagc
    10081 agccatcggt gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca ttcctattaa
    10141 cgctggattc gctccatgac taaatacgaa tggtgcaccg atcccaaccg tcatcacggt
    10201 gatggccgca aatgcattcc ccatgattac cgtaaatatc accatgccaa gagcgtaaat
    10261 gatgatgccg atgttcacat ttccttctgg aacgatcgta cctactgcat tggaaatcac
    10321 tgtcccaact ccagcgcttg taaataccgc acctagagaa gctaaaagca ttggcaacat
    10381 gcttaacggc cctactgtgc ccagcatatc tgctgcatca tctaaaaatg tacttggtcg
    10441 attatctcga gaaaagaaca tcaagattag catggctgca aaaacaccga cgcccacgcc
    10501 gactaaagca cctaaattgg taaatagagc aaacaagatc gcaaagacac cgatactcaa
    10561 agctggaata aaaattttca ttcctagttt gtctgacatt ttttccatat attcttttga
    10621 tggaagccgg ctttctcctt tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa
    10681 gatcaagcca ccgttcacga ttgccggaag ccatcttcct cctgcaataa ctacccctaa
    10741 gaccgtccag aaaaaggctg acccataacg atgaggattg gttttatctg ttaaatttct
    10801 tactccagtg taaatcaata cgagtcccat cacgatataa aggatctcca gtaatttatc
    10861 gcctaataaa atatcaggat ttgtaaaaaa gtccatctta ttgccccttt cctggataat
    10921 atttcttcgc taatttcttt tctttaagat agaagtagac acttgccaca actaaagccg
    10981 tgatagccac tggaacttcc actaaagcaa gctgtcccaa tgttacgtca tatcctagat
    11041 ctttcagtgt tgattggact agcaacgccc ctgctcctcc gatgaatagt acttggccaa
    11101 agaaccagca gacattttcc atagctgaag ccataccttt tagttcttct tcatgcttag
    11161 gatggacttc taaccctttt gattcgatgg tccccaatgc catcggtaaa atgattggac
    11221 gaacaaatcc agcaactcca ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga
    11281 tcccataaat atctacaatc ataccagcag atactttctt gaatcgcttg atcaaggaag
    11341 ccgctgattc tttcagacca tttctttcaa gcgttcctgt tgccaacata atgatgataa
    11401 aaatcgccat cccgcgattg tttacaaaac ttgtacctaa aagttctaac attccttgaa
    11461 tgccaagacc gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc aatatcgagt
    11521 ctaatttaaa tgcaaacccg agaataacaa tcaagacgcc taaaaggcta agataatttt
    11581 ccatatattt tcctccataa aatgacaata tttttttaat tataaagcaa aacacataaa
    11641 aaatatagtt gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt
    11701 atcacaaaaa ttatcttact tattgtaagg taaagctttt tttaatttat tgcccgataa
    11761 tatatagaaa taaggtctag ttactgtttt ttttaaatga ttaataaata acaacttctt
    11821 tctatgaaat tgcaaagcat tcatcttctt ttccgctaac gatatatccg tcagtgcttg
    11881 gtacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga
    11941 aaaagatata caatctgcca ccgggtagga taaagaggat gatcattcca ttaggtcttt
    12001 gaagggatga ttttcctctt tttgtgtatt tttttatagt taaggaggaa tgtaagtatg
    12061 gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa
    12121 tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg catgattgct
    12181 agtttttact ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc ttatcctatc
    12241 tggacaggga ttggcgcagt tggttcggtc ataattggtg tggtcttgtt tcatgataag
    12301 ctgaatcttt ccacttggtt ctttgtgggg atgctactca tcagtatcat tggcatcaag
    12361 attactagcg gccactagac acttggaata agtactgatc agaaaaaata agccttttta
    12421 tttaatattc aattttttta tgaaaaaata agaaataagt ccaatgacct agcgacttct
    12481 ccactcatca ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt
    12541 ctttcggtcc aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt
    12601 tttagcttat cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca
    12661 acgatcatga aagctatttt aggactgatc cattactcag atggaacaat ctgtattggt
    12721 gaaaatgaga tttctccctc tagtcataaa gggttggaac aagtaggagc attgatcgaa
    12781 tacccgggta tctatccatt cttaacaggc tatgatcatc tgaagttatt ctctgaaacc
    12841 aacgatgtat ctgctatcga cactatcgtg aatcagctca agatggaaaa gtacattcac
    12901 aaaaaagcga aatcctactc gttgggaatg aaacaaaaat tgggaattgc tcttgcctta
    12961 ctgaaccatc cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca
    13021 acaagagatg tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc
    13081 tccagtcact tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa
    13141 ggaaagatca tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc
    13201 gttatctcta caacaaatga tctagctgct cgaacgatct tgacagaagc tggatatact
    13261 ttggaagatc ctgataaaat caagctttta aaagaaaacg aagaattatt aggaaccgtg
    13321 atccagctat tgtctgcaca aaatatccat gtgacagacg tccagcatct gacgaatgat
    13381 ttggaatctt ctctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacat
    13441 tgcttgtaaa acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg
    13501 ttctattagt tgttttgctc ataggaacag cacttctagc caaaaaatac acgacgatca
    13561 ttgatccagt tgaaatgact gctcagcttt ttagtgccac atcttggatt gtctttatca
    13621 tgattgcagc tgcaagcacg atcatttcga tggaagcaca atatggtaca ttgaaaaatc
    13681 tcctttatcg aaagtattct cgaggagaaa tattagtaag caaatggatc acattagtta
    13741 tttattctgt ttatttatac cttttagcta ttattgtcac tgtgttgatg aaactgattc
    13801 ttttcccttc gatttccttc acggagaagg tttctactgg tcaaacactg atccaaagct
    13861 tgttcactta tactttgggt agttatatcg gtttatggct gattttaagt ttagtgttga
    13921 tgattgcttg ctttatcaat agttccggcg catccatctc agcaggaatc gtcttttatt
    13981 ttgcttcgtc aatcatatct ggtatcctta ttgcactcat ccaaaaatgg gagtggatca
    14041 aatggaatcc gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga
    14101 agcaattgac tcatctttct actaatcaaa tgctattcgg taatttagct tacatcgttt
    14161 tgttcttagc attaggatat ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa
    14221 aagaggctgt gaaagagacg ttcagcctta ttgccgattg gctgtctctt aaacaccgtt
    14281 tattctgaaa aaggagctgt gacaacattt gtgtcacagc tcctttccta gtatgttttt
    14341 gaataaacga caggctcaca acctctcttt atacttcttt tatacttcta tacgtgaagc
    14401 atcccagtgg caaataaaac cattgcaatc actccgacca tcgtcacacc tataagtaaa
    14461 atcttatcta agtggatatg ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa
    14521 aaataaatgc ctggcaagaa aagaacaaga acaagcatta cttcattaaa ggctacgact
    14581 agcatagccc atacttgaaa ggcacttgct aaaagcccta ctaaaaattg gacccattct
    14641 ttccgctgat aagaaagttt catttggtac aatcctaaga aaagccacgt gaagaaaata
    14701 gctgctgtac caagtgtgta agcaaagtta taagcactgt cagtgaataa taatgaaagt
    14761 aaaaagacat tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg
    14821 ttgagttttc cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca
    14881 atcggtaaga tcgtccatga gagccaaaca ccaactaaag aaatgatcaa accgccgttg
    14941 atcacgactg ctccccattt accaaccatc atttcaaaca catagcccat agcaggctgc
    15001 gccttgatcg tttgcagctg attcatcgtc attgtcccat atggaagcat ggaaaccaag
    15061 acgtaaataa taatcaagcc gaatatccct agaatcgttg ctttcccaac atctgattgt
    15121 ttttttgctc gtgaacctac gacagaggct ccttcaatac caacaaatac ccaaagcatc
    15181 accatgatgc aactcaatac ttgactggaa aacgggatcg gctgtccatt cgcattcaat
    15241 ataatgtcgc tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct gatagctact
    15301 acaataaata aaaataatgg cactaattta cagaatgtga cgatagcatt gacgaaacta
    15361 gcattttcga ctccccgatt cactagccat gcatatcccc ataaaaagac tgtactcatc
    15421 acgatcgatg ccagattttg cccaccttca aatactggga agaaatatcc taatgcgctc
    15481 atcaacaatg ttgcaaaagc tacgttgcct aaccagcatg tgagccagta accccaaccg
    15541 ctgataaaac cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt
    15601 aaatctggac gttttttatt gatattattg atcgacaagg agagcatcaa cattgctgtt
    15661 cctacgagta cccaactgaa aatagctggg ccgccagccg atccggagat gtccgtcata
    15721 ataccaaata cgccacctcc aatcgctgaa ccaattacta aggcagcaag tggaattaaa
    15781 ccaattcctt ttttctgttg ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt
    15841 ttatcaaaaa gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga
    15901 aatataattc taccggcttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa
    15961 ttacaccacc aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat
    16021 ccatgaacat tttatatata tcgatttcag gaaatacgcg tgcctttgcc aaacatctag
    16081 cagaatatgc tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag
    16141 aaatccatga aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta
    16201 cttatttaga cggcggaaat ggtttagata acggtgatac agaaatcttg accgaaacga
    16261 tgcgtgaata tctggaacat gaagacaatc ataaactttg tcttggtgta gtcggtagcg
    16321 gaaataaaaa cttcaataat cagtattgtc taacagccaa acaatatgca caaacatttg
    16381 gctttccttt cttggcagat tatgaattac ggggaacacc atctgatgtg acacgaattt
    16441 atcagatttt agcagaaaac aataaataat aaaaaagaag aagctgaaaa atagatttca
    16501 gtttcttctt tcgtttctat tgtttctatc atattaagct ttgacatcca tcacaggtgc
    16561 ttttgctttt gctttacctg ttttttccaa gacaaagttt tttacacgat ccatattggt
    16621 taccaagacg atcatggatg tttctttgcc tgctgctttg atcgcttcta agtcagcttc
    16681 tgctacaagc gtatcaggtg tcacttgctg cccttctgtt actttcacat caaatggcgt
    16741 accgcctaag tcaactgtgt tgattcccat atgaagcaag atttctaaac catttggcgt
    16801 tgtgatccca atagcgtgtt tcgttgggaa gacagagctg actaccccag taactggaga
    16861 atagatatgt ccgttttctg gttctacaaa gtagccttca cccatcatct tttgtgcaaa
    16921 aactggatca cttgctttat ctaaagaaac aactgttccg tcagcaactg atgagaggat
    16981 agtcacactt tcagctgaag tttgaggtac tactttttca ggcacttcat ttactgtggc
    17041 tgtttcagtc gctaattctt ctttttctaa acggtcttta ggaataccga agaagaatgt
    17101 agcaacgaag ccaccagcat aagctgccaa taatccaagg atgtagccta accatttacc
    17161 gtcagagatc aatgggatca aagcggctcc tgaaggtcca atagcgatag ccccgacatt
    17221 tccgatcatt ccgatcactg ccccgccgat acctccaccg atacaagcag taataaatgg
    17281 gcgtcctaga ggtaatgtta ctccgtaaat caatggttcg ccgattccta ggatcccgac
    17341 tggtaaagct cctttgatca tttctgttaa ttctttatct tttttgcagc gaatccataa
    17401 tgccaatgct gcacccactt gtcctgcacc agccatcgca agaattggaa gcaatagcgt
    17461 acgaccagtt tcatcaatca tttgcacatg gattggagtc aaaatttgat gcagtccgaa
    17521 cataaccatc ggcaagaata atgtgcctag aacgaagccg gcaaatgctc caccaacgcc
    17581 aagtacccag ttgatcccac cgaccatgcc atcagaaatg aaacctgcta aaggcatgat
    17641 gaagaaaatt tcaatcagac cgatggcaat caatgaaaga gttggtgtaa cgatgatatc
    17701 taccgcatca ggtactactt tatgcagttt tttctcaacg attgacaata gccaaacagc
    17761 gaagatgaca cctaggatcc caccttgtcc agctgcaaga gcttcaccag taaataagtt
    17821 tttcagtggt gcttccggat tcatccctgt cagcatcaca acggccccga taactccacc
    17881 aagagttggt gttgcaccaa aaacttgtgc agcattgata cctgtataga taactaggta
    17941 gctaaacatc ccgtttttca agatgttcat cacatcgata tattgttgcc atgttgctgc
    18001 atcaagtgtt ccggctgtga ctaagtttgc taaaactgcg gcaatccctg ccacgatccc
    18061 agtaccgaca aatgcaggaa tcattggcac aaagatatta gaaatgtctt ttaataccga
    18121 tttgatttta gatggttttt gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc
    18181 gtttacacgg tcttttccag atgctgatga atttgctgta gctgtactta cagaagaaag
    18241 tttttctcct aatttcacgc ctgccaaatc aaccatttgc tgtgctactt tgttcacttt
    18301 cccaggtccg atgatgactt gaagctgttc atcttctact acaccaagca ctcctggaat
    18361 cgcttttaat tcagagacat ttacaagatc atcattgatg atcttcatac gcacacgagt
    18421 catgcagtgg acaatgcttt ctacattgcc catgccgcct gcccctttat aaatttgttt
    18481 tgccagttct tccaaagtca gttcgttttt tgccatggtc ttttccccct cataaggtcc
    18541 cttttacaaa tccgtttgct tcagctaatt ttgcttgagc ttcctcttta tcactatttg
    18601 tcaaaatcat gacgatagcc agcttaacat tttgatctgc ttcttcaaag tacttttcag
    18661 ctgtttcgta atcagcttct gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt
    18721 tttcatttgt tggtttgaca tcgaccatca agttattgaa tactttacct ataccaatca
    18781 tactgatagt agaaatcata ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag
    18841 tagagcctgt caaaaattct ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac
    18901 tgatttcggc ttgtttattg caagaaatcg ttcctgtgac agcaccgatc gattttgcgt
    18961 aatctaatcc accgatcaca tatggtgtac gaccgctagc tgcgatacca ataactatat
    19021 cattttttgt caaattcagc ttgattagat cttcttgccc tagctcataa gaatcttcgg
    19081 ccccttcgac agctacagtc attgcttctt ctccaccagc aattagacct atgaccattt
    19141 ccggttctac accgaatgtc ggtacgcatt cagcagcatc taacacacct agtcttccac
    19201 ttgttccggc tcccatataa atcagacgtc cacctttttt aaaggactca atcgtgttag
    19261 caatcactgg ttcgatactt gtcagttctt tttcaaccgc agcggccact ttctgatctt
    19321 ctttgttcat tagttgaacg gcttgagaaa tactcatctc atctaatcca aaagtttctt
    19381 ggtttctgcg ttccgttgtt aggttctcta aattgatttt gctcattttt atcttccttc
    19441 ttttcttata aattgatagt tcataccagc tttgatttgg ttgatcaatg gtagctcttc
    19501 tttgataact ttagctacta cattcacttt ttcatccgct ggcaaatcat atttggttaa
    19561 ttggatctca cccatatagc gcaaatactt ttcgttatct aaagtaatag accctttcat
    19621 acgcgtagct gtatttcttg ctgggatagg cggaatctct cgaaaacgtg catctacgct
    19681 tcgtatgaca tctcttgcat cgtcttgccg attcgtatgc ttccctaata tatattcgta
    19741 aaattcttca tctacaggtt ccgtatggag caagactgtc tgttcttttt ggaaagaagc
    19801 aaattgctcc tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt
    19861 acttgctaat agatctagag cgcctgcaag aggatggatc cctcgatgtt cttccaaagt
    19921 tggcaatcct tgatatagtg ggcctctcaa ctctgtatct cctggtacaa aaccttggac
    19981 aaggagacct tgaattttga gccattgatt ttttttctga taccattctt tgtccagccc
    20041 tgtttcggga cgtggataat aattatgcca agcttcaatc tgtgagaaat cagcttctgc
    20101 ttctttcaat tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa
    20161 ttgatgtgac cagtcggcaa tctgctgatt ggaaatatga taatccattc ttaaacctgt
    20221 tacaccaatt gctttcaaag gcgttagatc ttctaatgaa aaacccgctc gttccaatgc
    20281 ctctcctgaa atatcgacca tcaattctag tttattcttt ttcgcttcgc ttcctagttc
    20341 gatcaatcgc ttttgatatg ccgaaacatc atcttctggt atatgcatgg aagtgaaaat
    20401 accgacaaag ccattggatg ccatttggcg gatatactgt ttcttctctt ctgacaaaga
    20461 atcgttcata aatattgaaa agccaaacat attatccctc tttttctgta tccgtcttca
    20521 tttctatgac tagagtatat atctaaagaa acaaaatttc aatactttta ttaaaataat
    20581 taaatttcat ttctaaaggt tgagtttata ttatcaaaag aattgtcata acctaaaaaa
    20641 ggcactgcta agcaaaagca gtgccttgaa atattttatt taaataacac aaaaacataa
    20701 ttactcataa gaaaccaatt ctgtattatc taccatgaat gtaaagaacc cctcattagg
    20761 catcggatga aaataagcat tgatcactct tatcaaggca ttatggcaaa ctaacagata
    20821 gacattttct tcgtcttcga tgcattcttt caataaagga acgatccgtg catacacatc
    20881 taagactgac tccccatttg ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt
    20941 ctcatctttg cttggcagtc catcataatc cccaaaatcc atttctacca atcgttcatc
    21001 aacggtcagc ggcaagctga gacgatcagc aactgcttgt gctgtatctc ttgctcgttg
    21061 aagaggggaa tggatgattt tcgtgattgg aacaggcaaa tctactacct tttctgctag
    21121 tttctgtgcc tgcatctgtc ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa
    21181 tccttgtgcg ttccaagttg tttctccgtg tctcgttaca taaagcatgt gattctctcc
    21241 ttcgttggca aagttcccct taaccatacc ttgttgttct tgagattaca agggttagat
    21301 tctctcaaga gaaataatct cagtagatac tttatccagg aaggtttgat catgttccac
    21361 tagtaacatg gcaggtttca cgttcaagat cagttgttct aattgttctt gattgaagac
    21421 atccaaataa ttcaatggtt catcccatgt atatagttca gctggctgtg ataaagattt
    21481 agccaattcc acttttttcc gttggcccat actcatctgc tcgatcttgt tatgaaaaac
    21541 atctctttcc atcccaagct ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt
    21601 tttctctgca aattccgcca acgttcctcg attgtcttca taattttggc ttgcataact
    21661 aatgctcaga tgttttggct ggtatttttc tcctataacc ttgccgttga atgcccccag
    21721 aagataatgg ataatggatg actttcctgc accgttagga cctgaaatcg ccacccgctg
    21781 atgaggctcg attgtaaaat gaattggctc gaataacaga ttttcatacc ctaattgaag
    21841 atcttctacg cttaaaagtc gcttatggtg agacgcttgg ctattcatcg tcaaagaatc
    21901 gatatattcg atatctttta atagtttttc tttctcgctg atctgggtct ccatccgatt
    21961 cacgattgct ttggatcgtt tcatcgtccg tgcagcatca gcacccactg ctcctttatt
    22021 cactcgtcta gattcagtat cgatgaatcc gacttgtttc tttgttttat ctccttctcg
    22081 ggaacgagac cattcggctt tttcagctgc tgttttcttt agcctactga cttctttttt
    22141 caatttttca ttttgagcca tttcaaattc atcacgaagt tttttctgtt cttcatagat
    22201 agagaaattc ccttgataaa gttccagttg acttttttcg attgctaaaa catggtccac
    22261 tacttcatcg ataaatcccc ggtcatggct gaccacgatg aagccttgtt ttttcttttt
    22321 caaataagcc gctacttgtt ttctaccaga gatatccaaa tgattcgttg gttcatcgat
    22381 taacgggaaa tgagtgtcat ccacaaataa aagtgccagt aggacttttg tcttctctcc
    22441 ccccgatagt gtgctgaatt ctctccataa gatttcagga tctgtttgca tcaattggag
    22501 ctctctttcg atttcccata tctcaaaatc cgtaatatca tttaacacgt aataggttaa
    22561 acgttcttta tcttttgtct gttgcgggaa ataggcaaat tcttgctgat ggattacttg
    22621 cccttgataa ggtagtttgt tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt
    22681 tcgtccgata agtcctagtt tccattgtgt gtcaaaattt agatttgctt gttcaaataa
    22741 taatgtgcct tggctgtcgt agccgaatgt cagatttttt atttcgattt tagacatttt
    22801 ccatcacctc gtaaaaaaag accagtccta ttttcagaac tggctaaaga ggatgcaagg
    22861 gttggataca cacaaaaaga cccatacatc gaaaaaacca agccaatccc gaaaatctgc
    22921 acactgcttc cttgtctcca caaggtacag tctgaatgca gctttcatca atcgacttaa
    22981 tttttcaatg cacggatccc tcactttgtc ctatatttga aattacgata gcataaagaa
    23041 aatgattttg tcaactactt cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag
    23101 atttttgttt tttctaatgt ttgcctgtgt tccattgctt cttctaaacc tattggaccc
    23161 aaatgaatac ttaagacaat tgggagaaaa tcagaaagtc cttctagctc ttggtctcta
    23221 cttccgcaaa tagcgattat tggttttcga tatttttttg caagcactgc tacaccgtat
    23281 ggaacttttc cgtggatcgt ttgctgatcg atccgccctt ctcctgtaat gatgacatct
    23341 gcttgttcga tctttttttc caatccagtg atttggctga ctaaatcgaa accagaaacc
    23401 atcttcgctc ccaacaaaaa aagggctcca ccaataccgc ctgctgctcc acttcctgga
    23461 atatcatcta aatccacctc ccagaaatct tttatttgct gagcaatctt tcctgcttgt
    23521 ttatctaaga aagctagcgt cgtatctatt cctccttttt gtcttccaaa ttgccaagca
    23581 gctccttgtt gacctgtata tggattggta acatctgcag cgataatcaa ttttacttta
    23641 gaaacaagaa cgtttcttcg atcaaaatga agttttttag tagataataa aggatttcct
    23701 tctgtgtacc cttctaaaga agcacccaag gattggagta atcctagacc accatcgcta
    23761 cacccgcttc ctcctaaagt gacaataatt tgttctactt cttgaagcag tgcatcttta
    23821 ataagttctc ctaaggcata actggaagca agtcggatag tttgatccgt tggtttatca
    23881 agtagatcta accccaatgc ttgtgcagat tcgatgactg cggtgttttt tccttgccaa
    23941 gttgtcaata aatagctgat ttttcgctga cggaataaga gatccatccc atcaatggta
    24001 cgccatgttc ccctgagcgc gtaatagatt gcttctgcag tattttctcc gccatctgca
    24061 atcggcacat tgatttttct tcctttacga tcttcccagc catttagaac tgcctgattt
    24121 agttcaaatg aagaagcact tcctttaaat gaatcaattg ccgccaaaat atccatctgt
    24181 atccctcact ttcttcttaa gtaagacact ttttttcatt tttttcaaac aaaacaatgc
    24241 tattttcttc catttttcta tatactaaaa gggaactcat tgaataaagg agagaaaaaa
    24301 catgaaaatc gcaatagctg gggccggagc aatgggcagc cgaatcggat tgatgctaca
    24361 tcaaagcgga aatgaggtcc tcttgatcga tcgttggcct gctcacattg aagccattcg
    24421 tacaaatggg ttgattgctg actttaacgg taaagaagtg gtcgcaaaac tgccaatcta
    24481 ttcaccagaa gaaatcatcg aaagcaacga acacgtggac ttgatcgtag cattaacaaa
    24541 agccaatcaa ttagatgata tgttttgttc gatccaatcc attattactg acaatactta
    24601 tgttttgtgt ttactaaatg gattaggtca tgaagatgta ctggaaaaat atgtgccaaa
    24661 gaaaaatatc ttatttggga tcacgatgtg gacagctggt ctagctggtc ccggaaaagt
    24721 cacattgtta ggtgatggag aaatcgaact agagaacttg gaaccagaag gcgaagcatt
    24781 tacaaaaaaa gttgtagaag tctttcaaga agcaaattta aatccgatct atagccataa
    24841 cgttcgttat tccatttggc gaaaagcttg tgtaaatgga acattgaatg gtttatgcac
    24901 gattttggat tgtaatattg ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg
    24961 aacaatcgta tctgaatttg cttctatcgc agcaaaagaa ggaatcatac tagatcaaga
    25021 agaagtgtac caacatattg cttctactta tgatcctgac aacatcgggt tgcattaccc
    25081 atctatgtac caagacctga tcaaaaacca tcgtttgact gaaatcgact acatcaatgg
    25141 tgccatttgg cgaaaaggac aaaaatatga tattgctaca ccatattgtg cctttttgac
    25201 ccaattagtc catgcaaaag aaggaatcct tggcgcagaa taaaaggctc tctgatgaga
    25261 atcagctgaa tcagcagctg gttctctttt tttaagccca ataaaagatc aaaaagaaaa
    25321 cacaactctc atgatgccat aagaattgtg cttgtttgtt tcagtacata atctcttcaa
    25381 aatgtaggtt ttttatttgt tttcaacaga ggagaggtca tattcgtatt gctcataaat
    25441 agtggaatcg ttagtgataa atcgttttgc ccaattttct gcatcataaa attccaatag
    25501 ctctgatagg attggcttaa atggtgtatg cagaaattcc ggtaattcat ctaaatcacg
    25561 ttctcgaagc aaaaaaatat tttcaggacg ataaaaatga taatgagcaa tcgcttgatt
    25621 atccgtcact agtttctttt gaaaatacat ggattcaaat gtccgcaaag tcactccttg
    25681 ctgaccctcc cgcaacagtt ccagaatccc gcgcgattcc cctgtttttt tcaggtactc
    25741 ttcataagta agatatccag gttgttcatt ccctcgatgt ggaagaatga aatagttggt
    25801 cgtaacatct ctttttataa attcctgctt atagctttct gctcgttctt ttctcccatt
    25861 attcgttgca ccaaaaaata gatccgtctt tggttcgcta gaagaaaaat tcatatgttt
    25921 cgaataaaat gaactattat gtttcagccc aagttctttt gcttccaaaa aatcaaaatg
    25981 ataaatctca tcgattgccg gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc
    26041 tttgaagtga ttccaaaaaa ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg
    26101 agccatctcc atatcaaatc cataatctaa aaaaacgatc agctgataat ttgccaactg
    26161 ttgtctagct gatttaatga attccgactg tagtaaatct tttccggacc aaacatctaa
    26221 agagcggatg gaaggcaaaa aataaagtga tttatttttt gcaactagca gtacgtcatt
    26281 tttgttcatc ttttccgcct cactttctct gtcacttttt cctatgatag cctgtttaaa
    26341 tagtaaaaag ccagtaaaac gtttcctata tttgtatgaa aatttaacat ttgtatgaaa
    26401 atatgatgaa ataaatcatt cttctattaa aattcgccta aaagttttct gaatagttgt
    26461 aattgtctga aaatttgtta tcattagtca ttgttaaata ttgctattta ttcttaatat
    26521 aaattttgat tgtgaattta aaagaaagag gtcatttatg gaagataaaa aattgaaaaa
    26581 acaacttagc tccagacata tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct
    26641 atttaaagga agtggcgaag cgattgggat cgccggccct tctgtattga ttgctttttt
    26701 gattggtggg ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg
    26761 tggcgacacg catcacggat tatctggttt gatacgccct tatctaggtg ctcattctgc
    26821 tgattttact gattgggtct actggtctat gtggattatc aatatcatcg ctgaagctgt
    26881 tgcagctgct tcctttttgc agttgtggtt cccgcacatt ccagcctggg tatttgtttt
    26941 tatgttggct gttttaacta cgatcatcaa tctttattcg gtacggttat tcgcagaaac
    27001 agaatattgg ctggcttttg ctaaaatcag tgtcattatc ttactgatca tttttgctac
    27061 ttatcttgtc ggtcagcaaa tgttgggcac aggcgttttc cctacccttc agcaattgac
    27121 tgaccatggt gggtttactc ctcatggtat gaagggaatc atcagttctt tgcttgtagt
    27181 tatctattct tacggcggtt ctgaactgat tgctatcacg gtaagcgaag cagatgatcc
    27241 taaacgtgcg attcctaaag caatcaaagg tgtcatggga agaatcattt ccttttacat
    27301 cattcctttg tttttgcttt tgattctttt tccttggaat actttagcag gcacgaatgt
    27361 cagtcctttt gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa
    27421 ttttgtgatt gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg catcttcaag
    27481 aattttgttc ttccgtttga aggatcgaaa aggttcctct agaaaactag ctgttttaaa
    27541 caagcatcaa gtaccgcaac gtgctgtttt attttgtacc agtgttcttt atcttggtgt
    27601 agctctttct tattttgtcg gtgacaaatt atttggctat ctggcaggtt ccctttcgta
    27661 tacagtatta ttgatctgga tcattatcag tgcggcatcc tttgtactgg cactaagaaa
    27721 aggaacatta tgggataaag gaatcagctt ttttgcatta gctgtcttag gattgatttt
    27781 ccttggtatc ctgttcacga actccattgg ggtaactgta ttgactggcc tgctttacct
    27841 gttcatttat ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa
    27901 tcgatagaaa attttatttt atttatgtgg aatagatgga gctattgaag aaaggagttg
    27961 tgacaaaagt cttgttgcaa ctcctttttc cgaataaaca gtctcatgac ctcttggtaa
    28021 acggtgttca aaaaccctct gtgcaaaata attgattgtt ctccatgctc atgacatgat
    28081 agaagaaagg aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt
    28141 tgatgatcgg tgtttcaacg gtagttatgt ttggtatgat gtacttgaat gtctatgcgc
    28201 tcgatcatat cttcttcagt cgtacacgag tattcatggc gttgatgatg ggtgcagtca
    28261 tggcagtcat catgctgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta
    28321 tgatcttagc cgctagtctt cttgtttttg ccggttcctt gtttatggta cgaagccaaa
    28381 ctactgtcaa cgacacagct tggatgaagg ccatgattcc tcatcattct atcgctatcc
    28441 tggcaagtaa acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca
    28501 ttcaggcaca agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac
    28561 cattatccgt ccaaataaaa aaagaggatg ttccagaagt tatcagcttc aagaaataag
    28621 aagaagctac ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag
    28681 taataagaac agccaaacaa aaatagcttc tcctattttt cgtttggtta ggcttattac
    28741 cgcagatgca agcttttgaa caccgtttat taagaggttg taaaatcagc gttctgacct
    28801 gagtattaga gagacaaatg gaaaaatagt tcctcatatt ttgccatttt ttgagctaat
    28861 accgagggtc agctgattga acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt
    28921 cgcgtattaa gaaaaatttt gtgaaatggc tttccacatt tttgtataaa tttggcttaa
    28981 taccgagaaa ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt
    29041 gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt
    29101 gcggtttcac aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca
    29161 caggagaaag caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg
    29221 tacttgctac gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac
    29281 catcttctag cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt
    29341 taaatgcatt accaggcaga ataacagata ggaattgttc accaataaaa atatttactc
    29401 cgatacttgt catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata
    29461 ctcgttccat cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag
    29521 ttagaataat caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg
    29581 aatcgatttg ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact
    29641 tcgttaagga agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca
    29701 acaaaatcgt taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag
    29761 gaatcagtga ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa
    29821 tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa
    29881 caacaaatgc cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat
    29941 caacaatagc tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa
    30001 cggcacctga aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa
    30061 taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac
    30121 ctacgattgc acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga
    30181 acccaatgac cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac
    30241 cgactaaaat aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt
    30301 cgtgcagcca atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg
    30361 ctgctaagat cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta
    30421 aaacaataac cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca
    30481 tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac
    30541 tccgccgcaa ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta
    30601 aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa
    30661 cttttattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca
    30721 gcgaccaccg actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta
    30781 cgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc
    30841 tgaaaattat ttttataact ttcacacaca agtttatttt ttactagaaa gtagcgccct
    30901 cttgtttttt tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg
    30961 gctctttcaa cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag
    31021 caaggccttt gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg
    31081 tctcagagat tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat
    31141 cagaagaacc gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt
    31201 cttttgcatg ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg
    31261 cattttggaa caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga
    31321 tattttccac tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga
    31381 aacgtccagc agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc
    31441 catggatatt gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt
    31501 ggatcaatgc ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga
    31561 caccgtaaaa tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac
    31621 gaaaagctgg atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa
    31681 agctgccttt agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga
    31741 tttctccacc agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag
    31801 taccaaacaa attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca
    31861 aaaaggcaat tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt
    31921 gtccagctac ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg
    31981 ctgaggaata aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg
    32041 cttgatattc tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg
    32101 ttgtcataaa aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc
    32161 atgcaggcac gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt
    32221 gataagcgtc tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga
    32281 tgatattcac ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag
    32341 caggattaaa tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg
    32401 acaaagcacg tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct
    32461 cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag
    32521 cagtttgcaa taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt
    32581 ttgctccgtc tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg
    32641 cattgacagt atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta
    32701 aaaagaattg atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg
    32761 gttgattgta atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt
    32821 ctttcacgtt gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt
    32881 cttttttgtc agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta
    32941 caaatgtaat gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt
    33001 gtaatttttt taatcagttg attactgata aattgactta aaaaaactaa gattagaatc
    33061 aataatgtag ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca
    33121 agattcccta atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg
    33181 atgatagtca acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt
    33241 aaaatgatac cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca
    33301 acttctagca gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat
    33361 ggcactaaag ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct
    33421 aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt
    33481 atttcaaata gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca
    33541 cctaagatga ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt
    33601 tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta
    33661 aagaaatcag tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt
    33721 tttttgtaaa atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg
    33781 ctgtttggtt tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa
    33841 ctctacattg ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat
    33901 tagaggttcg ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat
    33961 ctgatcggca aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc
    34021 tgttcgaata aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct
    34081 acctttttca acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac
    34141 aacttgcatc tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt
    34201 taacaatgca aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca
    34261 taataatacc ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc
    34321 ccccgacagt tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag
    34381 ttcattcact ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc
    34441 aacgttgtca aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat
    34501 ggcctttctt tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac
    34561 aatgacttct cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt
    34621 ccctgcacct gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac
    34681 agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa
    34741 ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca
    34801 ttttcttgag gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca
    34861 actcctcttc aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta
    34921 catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt
    34981 ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa
    35041 taatattcaa aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta
    35101 tcgcggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga
    35161 gattggcgtt cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat
    35221 tctccaaggc cattttcagc ttttcttact tgactcttct caccagccag agcctctctg
    35281 caaagtttcc aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat
    35341 ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt
    35401 tttctgtttt tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa
    35461 gtttcaattt ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca
    35521 aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg
    35581 caaaggctga actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg
    35641 aaggcttgtt ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt
    35701 taaagtgatc caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt
    35761 tcatatgacg ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg
    35821 ctccgcttgt cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt
    35881 tttcagggct acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc
    35941 cccaagcgtc aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc
    36001 agccatcacg atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac
    36061 ctttcatcta tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca
    36121 aaaagaacag aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat
    36181 atagattttc ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca
    36241 taaccagtaa ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg
    36301 aaggacagaa ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt
    36361 gctttggata aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca
    36421 tagtgattcg acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact
    36481 atccaatgta tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa
    36541 gtaagtagta atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat
    36601 actagatgca ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg
    36661 caaggaaatg caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat
    36721 tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga
    36781 acttgctgta gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag
    36841 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc
    36901 aatatccaca gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc
    36961 attgatacca tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa
    37021 acgtgctttt tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc
    37081 taattcatga tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag
    37141 acctacttga cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac
    37201 agctacacca tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc
    37261 aaggaaagca agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc
    37321 aacgacgctt ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag
    37381 tgactgtact tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc
    37441 gccaagatca ataaatttag aaatgttcag catttcttca atcgcgcctt ttgtgatcat
    37501 ttgtgtttta ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa
    37561 aggaatttca tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc
    37621 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata
    37681 cgcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
    37741 gattttatct tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa
    37801 gttctggatg gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc
    37861 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac
    37921 agatagtcca aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat
    37981 aactgctgga gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc
    38041 taattcaaag cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag
    38101 tgtttcattt ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc
    38161 cataaagaca agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc
    38221 atgcttttct actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt
    38281 tgcggacaat aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc
    38341 tccacagacg atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc
    38401 cgttactttc acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat
    38461 caacgtcatt gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc
    38521 tttatctcca ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat
    38581 caacactaat gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt
    38641 tttgccatat gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc
    38701 tgtcaatcca tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc
    38761 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg
    38821 tccaaaagac agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga
    38881 aaaaactgaa aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt
    38941 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    39001 tgacttccaa tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt
    39061 tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt
    39121 gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac
    39181 gtaaaaagca gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg
    39241 ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct
    39301 cacctaacaa atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc
    39361 aaatcaattt tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc
    39421 acgcggtttt atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg
    39481 aggtgctgtt atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt
    39541 agtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt
    39601 tgaaactatg actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt
    39661 ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg
    39721 cgaggaagtg atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt
    39781 tccaggtacg atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc
    39841 tgattcccgt gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa
    39901 caaaccgtct gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg
    39961 gttcagcaat ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat
    40021 attcgagcac tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag
    40081 aaatcaatct ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt
    40141 tatgtgataa gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat
    40201 tttctatata aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt
    40261 tcaactgtgc taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc
    40321 gatcactgaa tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg
    40381 gtatctgcat caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg
    40441 tctcagatac ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt
    40501 ctgttttctt aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt
    40561 cttgtacccg atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt
    40621 ttcgcatcca ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg
    40681 ttacaaatga catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac
    40741 agattccttc cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca
    40801 attcaccatc atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat
    40861 agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa
    40921 taccaacctt caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt
    40981 gattattttt ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt
    41041 tattaccatt ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt
    41101 cattacgttc cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    41161 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    41221 tcttgacttt ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga
    41281 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca
    41341 caaagttccc atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac
    41401 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    41461 cgatttctcc accgtattca tgattgactg tccctttagt ataaccaggc atatggatca
    41521 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    41581 caagagcgct tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    41641 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    41701 atgattgatc cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat
    41761 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    41821 aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga
    41881 ataaatcttt atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc
    41941 gatcaaagaa attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag
    42001 ctacattccc atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg
    42061 cggcttgaaa gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    42121 atgtgccagt ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    42181 agaaattgtc aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga
    42241 cttcttgtcc gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa
    42301 cgatcacatt tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt
    42361 tctttgtata atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc
    42421 tagtcattcc tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga
    42481 caatatatga acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg
    42541 ctagatttcc tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc
    42601 cgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    42661 acacagcgat atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc
    42721 ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    42781 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    42841 tcaaaagaat agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    42901 ataaggtagt cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac
    42961 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    43021 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    43081 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    43141 gttatcttaa gattttttta ttctttctaa agattattcc gtccactttt cacagcataa
    43201 aaccgctgcg atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt
    43261 cactccacgg tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca
    43321 aaattcttgt cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct
    43381 taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga
    43441 agctgttttt tgaaattgtt ccttgtggct cagagttaaa cggcaggctc aagacctctt
    43501 gatacacggt gttcaatcag ctgaccctcg gtattagatt atgtcaggac gctaatttta
    43561 caacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
    43621 aaaaatataa ggagctattt tttgaaa
//
LOCUS       DACEJZ010000011        42920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEJZ010000011 DACEJZ010000000
VERSION     DACEJZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.011"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..423
                     /locus_tag="IW656_000587"
     CDS             139..423
                     /locus_tag="IW656_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0836315.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(704..1123)
                     /locus_tag="IW656_000588"
     CDS             complement(704..1123)
                     /locus_tag="IW656_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0836316.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1125..1709)
                     /locus_tag="IW656_000589"
     CDS             complement(1125..1709)
                     /locus_tag="IW656_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0836317.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1756..3231)
                     /locus_tag="IW656_000590"
     CDS             complement(1756..3231)
                     /locus_tag="IW656_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0836318.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3412..3723
                     /locus_tag="IW656_000591"
     CDS             3412..3723
                     /locus_tag="IW656_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836319.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3838..6174)
                     /locus_tag="IW656_000592"
     CDS             complement(3838..6174)
                     /locus_tag="IW656_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0836320.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6316..6984
                     /locus_tag="IW656_000593"
     CDS             6316..6984
                     /locus_tag="IW656_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836321.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6987..8030
                     /locus_tag="IW656_000594"
     CDS             6987..8030
                     /locus_tag="IW656_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0836322.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8267..9148)
                     /locus_tag="IW656_000595"
     CDS             complement(8267..9148)
                     /locus_tag="IW656_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0836323.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9307..10035
                     /locus_tag="IW656_000596"
     CDS             9307..10035
                     /locus_tag="IW656_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0836324.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10191..10508
                     /locus_tag="IW656_000597"
     CDS             10191..10508
                     /locus_tag="IW656_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0836325.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10501..11109
                     /locus_tag="IW656_000598"
     CDS             10501..11109
                     /locus_tag="IW656_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0836326.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11125..11955
                     /locus_tag="IW656_000599"
     CDS             11125..11955
                     /locus_tag="IW656_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0836327.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12093..12581
                     /locus_tag="IW656_000600"
     CDS             12093..12581
                     /locus_tag="IW656_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0836328.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12632..13504
                     /locus_tag="IW656_000601"
     CDS             12632..13504
                     /locus_tag="IW656_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0836329.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13680..14042
                     /locus_tag="IW656_000602"
     CDS             13680..14042
                     /locus_tag="IW656_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0836330.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14051..14803
                     /locus_tag="IW656_000603"
     CDS             14051..14803
                     /locus_tag="IW656_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836331.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14817..16619
                     /locus_tag="IW656_000604"
     CDS             14817..16619
                     /locus_tag="IW656_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836332.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16896..17936
                     /locus_tag="IW656_000605"
     CDS             16896..17936
                     /locus_tag="IW656_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0836333.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17988..20132)
                     /locus_tag="IW656_000606"
     CDS             complement(17988..20132)
                     /locus_tag="IW656_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0836334.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20489..21862)
                     /locus_tag="IW656_000607"
     CDS             complement(20489..21862)
                     /locus_tag="IW656_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0836335.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21862..22257)
                     /gene="rbsD"
                     /locus_tag="IW656_000608"
     CDS             complement(21862..22257)
                     /gene="rbsD"
                     /locus_tag="IW656_000608"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0836336.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22285..23202)
                     /gene="rbsK"
                     /locus_tag="IW656_000609"
     CDS             complement(22285..23202)
                     /gene="rbsK"
                     /locus_tag="IW656_000609"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0836337.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23310..24323)
                     /locus_tag="IW656_000610"
     CDS             complement(23310..24323)
                     /locus_tag="IW656_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0836338.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24404..25003)
                     /locus_tag="IW656_000611"
     CDS             complement(24404..25003)
                     /locus_tag="IW656_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0836339.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25189..25890
                     /locus_tag="IW656_000612"
     CDS             25189..25890
                     /locus_tag="IW656_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836340.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25900..28611
                     /locus_tag="IW656_000613"
     CDS             25900..28611
                     /locus_tag="IW656_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0836341.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29062..30690
                     /gene="treC"
                     /locus_tag="IW656_000614"
     CDS             29062..30690
                     /gene="treC"
                     /locus_tag="IW656_000614"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0836342.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30750..31931)
                     /locus_tag="IW656_000615"
     CDS             complement(30750..31931)
                     /locus_tag="IW656_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0836343.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32183..33706
                     /locus_tag="IW656_000616"
     CDS             32183..33706
                     /locus_tag="IW656_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0836344.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33775..34503)
                     /locus_tag="IW656_000617"
     CDS             complement(33775..34503)
                     /locus_tag="IW656_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836345.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34516..35796)
                     /locus_tag="IW656_000618"
     CDS             complement(34516..35796)
                     /locus_tag="IW656_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0836346.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(35937..36539)
                     /locus_tag="IW656_000619"
     CDS             complement(35937..36539)
                     /locus_tag="IW656_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0836347.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36784..36990
                     /locus_tag="IW656_000620"
     CDS             36784..36990
                     /locus_tag="IW656_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836348.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37302..38021
                     /locus_tag="IW656_000621"
     CDS             37302..38021
                     /locus_tag="IW656_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0836349.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38102..38785)
                     /locus_tag="IW656_000622"
     CDS             complement(38102..38785)
                     /locus_tag="IW656_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0836350.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39003..41189
                     /locus_tag="IW656_000623"
     CDS             39003..41189
                     /locus_tag="IW656_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0836351.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            41535..41945
                     /locus_tag="IW656_000624"
     CDS             41535..41945
                     /locus_tag="IW656_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0836352.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            41959..42315
                     /locus_tag="IW656_000625"
     CDS             41959..42315
                     /locus_tag="IW656_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0836353.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        14001 a         7550 c         8575 g        12794 t
ORIGIN      
        1 tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg
       61 aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat actagatgta
      121 aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc
      181 gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt
      241 gctggaagag ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg
      301 aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat
      361 attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa
      421 taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca
      481 gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt
      541 tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag
      601 ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt
      661 ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac
      721 catctttata ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa
      781 tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat
      841 ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca
      901 cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac
      961 gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat
     1021 ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg
     1081 tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc accctgccaa
     1141 cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta
     1201 acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc
     1261 acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca
     1321 cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc
     1381 aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg
     1441 atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa
     1501 taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag
     1561 ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt
     1621 ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt
     1681 tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca
     1741 gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc
     1801 acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact
     1861 tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg
     1921 atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa
     1981 agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt
     2041 cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc
     2101 cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg
     2161 attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc
     2221 ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat
     2281 gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt
     2341 agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc
     2401 ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat
     2461 acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc
     2521 tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat
     2581 ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc
     2641 gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt
     2701 atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc
     2761 tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg
     2821 tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg
     2881 tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt
     2941 caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact
     3001 agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat
     3061 agctggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac
     3121 accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag
     3181 tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc
     3241 ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact
     3301 tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat
     3361 gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa
     3421 atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt
     3481 acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat
     3541 gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt
     3601 aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga
     3661 aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta
     3721 taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc
     3781 tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta
     3841 ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc
     3901 gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag
     3961 ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc
     4021 tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc
     4081 gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc
     4141 ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac
     4201 taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt
     4261 cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc
     4321 tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata
     4381 aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc
     4441 attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac
     4501 gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat
     4561 tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt
     4621 caattcatca gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc
     4681 agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat
     4741 atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt
     4801 gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata
     4861 acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg
     4921 tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag
     4981 agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc
     5041 acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc
     5101 gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc
     5161 ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc
     5221 caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt
     5281 cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag
     5341 aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc
     5401 tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt
     5461 tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa
     5521 gatcttacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt
     5581 atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg
     5641 gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc
     5701 attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg
     5761 ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg
     5821 tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc
     5881 tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc
     5941 atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga
     6001 atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg
     6061 acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt
     6121 ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac
     6181 ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt
     6241 atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa
     6301 aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg
     6361 ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag
     6421 gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg
     6481 ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct
     6541 gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga
     6601 gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca
     6661 ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta
     6721 gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa
     6781 tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg
     6841 atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc
     6901 aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga
     6961 ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct
     7021 attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta
     7081 ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat
     7141 acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg
     7201 ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa
     7261 cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt
     7321 ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact
     7381 ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc
     7441 aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta
     7501 tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg
     7561 aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag
     7621 taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg
     7681 ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg
     7741 ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag
     7801 ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag
     7861 atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc
     7921 taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca
     7981 gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat
     8041 aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta
     8101 gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag
     8161 aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc
     8221 gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt
     8281 gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa
     8341 atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata
     8401 gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga
     8461 tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac
     8521 acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct
     8581 ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa
     8641 ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga
     8701 aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga
     8761 cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga
     8821 ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat
     8881 tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat
     8941 tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc
     9001 gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc
     9061 cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa
     9121 gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca ataaagaata
     9181 ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc
     9241 gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt
     9301 tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt
     9361 ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt
     9421 ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct
     9481 gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg
     9541 ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag
     9601 cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt
     9661 gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt
     9721 ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg
     9781 tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg
     9841 gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc
     9901 ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct
     9961 gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt
    10021 atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa
    10081 atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt
    10141 ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc
    10201 agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt
    10261 atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat
    10321 atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg
    10381 aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa
    10441 ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa
    10501 atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt
    10561 tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag
    10621 atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt
    10681 tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg
    10741 atttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg
    10801 tttatattgg ttattggggg actattggtt gctttactag cgctcatttt tagtatctat
    10861 gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg
    10921 tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga
    10981 agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg
    11041 atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg
    11101 ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt
    11161 ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt
    11221 ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag
    11281 gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg
    11341 gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat
    11401 ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac
    11461 acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc
    11521 gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag
    11581 acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat
    11641 gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa
    11701 aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac
    11761 ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt
    11821 gaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag
    11881 aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac
    11941 aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct
    12001 tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa
    12061 atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa
    12121 agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct
    12181 atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat
    12241 tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt
    12301 attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat
    12361 ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct
    12421 tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac
    12481 cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag
    12541 aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt
    12601 tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa
    12661 tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta
    12721 tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat
    12781 cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt
    12841 gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt
    12901 ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt
    12961 caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga
    13021 tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga
    13081 gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt
    13141 gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg
    13201 tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac
    13261 agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca
    13321 aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg
    13381 acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc
    13441 aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa
    13501 gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc
    13561 tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa
    13621 ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag
    13681 tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa
    13741 tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta
    13801 caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc
    13861 ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac
    13921 cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt
    13981 atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt
    14041 agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa
    14101 caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt
    14161 tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac
    14221 attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa
    14281 aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa
    14341 tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt
    14401 cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca
    14461 tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc
    14521 acgtgcattg atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga
    14581 ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt
    14641 atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt
    14701 catcaaggat gtcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt
    14761 ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc
    14821 tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct
    14881 caggattagt tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag
    14941 cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg
    15001 ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga
    15061 tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac
    15121 agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg
    15181 tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata
    15241 tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg
    15301 ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag
    15361 cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc
    15421 ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg
    15481 ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt
    15541 tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt
    15601 cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata
    15661 cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag
    15721 gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag
    15781 tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt
    15841 ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg
    15901 cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag
    15961 aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg
    16021 aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac
    16081 tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga
    16141 aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag
    16201 agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg
    16261 aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt
    16321 tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag
    16381 gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag
    16441 caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca
    16501 tgcaaaatcc atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca
    16561 tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa
    16621 aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc
    16681 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga
    16741 ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa
    16801 attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg tatactggaa
    16861 tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg
    16921 gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg
    16981 atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga
    17041 tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa
    17101 tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg
    17161 aagtaaaaac agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag
    17221 ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc
    17281 agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata
    17341 cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag
    17401 tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg
    17461 acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc
    17521 ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat
    17581 taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc
    17641 catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg
    17701 aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt
    17761 ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat
    17821 ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg
    17881 aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata
    17941 aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa
    18001 ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca
    18061 gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa
    18121 tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa
    18181 aattctaatc ctgtgattgg cacgccatct gttgtccatt tgattggcac atcgaatcgc
    18241 ttagacatat tgaggatttc agcgtttgct tcttcagttg ttgctgcttc ttttttcggt
    18301 tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg
    18361 cgatctgtag cataacgtcc ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc
    18421 gacttccaag ttggtttgta gaaatcagga ttgccagtca gtccattttt gatcaagtcg
    18481 gcatagtctt ccaaactttc cttgtaagaa ggatatttac ggaattgtgc gcggatttgg
    18541 tagctttgtc cggaactatc ttgttcaagt gtattgaagg tcacgctgct tcctttatat
    18601 gatcctttga taccaaaaag gttatgattt ggttcgcttg ataataagct gtttccagat
    18661 ccactttcaa ggatagcttg agccatcatg actgatgcgt agattccttc tttttgcccg
    18721 atttcctgtg catcatctgc aatctttttc aagaactgat cagttgtttg gttttgactg
    18781 aaatggaacg agccatctaa tccatctaaa atagatgagc cgcctgtcag cgaacctgaa
    18841 gcagcaggta aacctgctaa gtttactcgg acagcaaaat caggcgtgaa atctgaaata
    18901 gaagttactc gaactttatc tcctggttga ggcgcatgga taaattgtcc gttcccaata
    18961 tagataccta catggtgagt aacaccggaa gaaggtcccc agaagacgag gtcgccaggt
    19021 tgggcctgac ttactggaat ctgcgtacct gcatattgct gttcgcctgt ccagccaccg
    19081 atataatgac cggttgcttt catatatacg taataagtca aaccagaaca gtcaaaagta
    19141 tttggacctt ttgctcccca tacatatggt tttccaagat gcttgaatgc ttctcgaacg
    19201 attgcttgtt ggacattcgt tgtatttgta ggcaatacag catcaattgg tgattttaca
    19261 acaaaatcag aagaagcaga agatggattc tctataccgc tttgatttgc tgtatttgga
    19321 cgagatgaag cattgttcgt tccgccattc gatgaaccat tgtttggatg gtttggttct
    19381 ctctgagtat ttccattgtt attcgatcca gatgggtttg tttgattcga agaattcccg
    19441 ctgttcgaat tattgttatt attgtttgaa ttatttgaac cattattatt gttgttatta
    19501 cccacatctg gtttgtttgg cgtttccgga tctggtgtac tagggacttc ttcactagga
    19561 tcagttggtt tctctgttga agtatccggt ccagctttaa attctgcagt aatagataaa
    19621 ttgttcgtac ctactgtaaa ggtctttgca tcataattta cttctttggc accattaaca
    19681 gcgactgaag caatttgttc accttcagct ggcgtaatag tgtaagttac cttcgtttcc
    19741 gctaataaac cttctaccgt tccactttcc gtaatatcca aaggttgctg ttcaggggaa
    19801 tccgctgtcg aagcagtcag ttggatctta ccaatcaaag aaggagcaat tgtaagggaa
    19861 tatgtctcgg ctggcttttc attttctgat ggatcagccg gatcaatttc agttggctcg
    19921 gtcgatcctg ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga atccgttgtt
    19981 tcactagtag ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc agctatttga
    20041 ttatctacgg caaatacggt attggtcgac aaaacagacg gagcaatcag ccaagtagca
    20101 gctagcaaac gtatcgtttt tttatttttc ataataaact agtccttttc ctttcattga
    20161 ttttctgtct tattcagcat tcattagaca ttttttggaa cagataatcg tgtcgaaaag
    20221 ctttttcttt tcataaatct gtctgtgtat tctaaataaa acaatttgta aataagcaac
    20281 gagcattcat tcgtttttct ttgacgttca ttttattgca tagaccacct ttgtttgggg
    20341 ataaacaagt ggggataaaa atggttatgc atatgtaaat tataccaaag tttggaaagt
    20401 atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa agactgaaat atgtgataaa
    20461 catctcatac ttcagtcttt ttcattaact attcataaga cattaggtct tcatcaaacc
    20521 cgctaacgag agtagtgatc attgctacga ctagactaac agccagtcct gctaagtacc
    20581 agaaaaaagt accggaaatg aatgcaggca atgtggtcag actaccaaat acaaaagcga
    20641 tcgctttcac ttgggcaaat cccattacag ctccaccagc agctgcacca atgcaagcag
    20701 caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac agttggttcg gtcactcccg
    20761 caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc
    20821 gcagaaatac acctaaagct gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa
    20881 cgattcggtc gaacccataa gcagagatat tactgatcat gacagggttt acagcccatt
    20941 ggataccaaa tattaccaaa atattccaac caccagcgat gattgcgcca ctgatcggtc
    21001 cgttaatatc catcatccaa gtaattgcgg cagctaaacc ttcgccaagg taaacacccg
    21061 caggcccgaa taccattaac gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa
    21121 atactaattg catattttcg cgaatatatt ttttgaggaa tttttcaaca aaagaataaa
    21181 agaaaatacc taaaatcgcc ggcatgacag tagacgtata ctgcatcagt actacaggaa
    21241 tccccagaaa atcagtagct gctttgccac tgtctgaaag taaagaggtc agattcggtt
    21301 caatcagtgc tgcagcaatg acagccgcaa tatatccatt cactttaaac gttttggcag
    21361 cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc attcgacgca gcagacaaaa
    21421 tatgataagt tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa acaaccagac
    21481 cttttaatat accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca
    21541 aaccaaatag tttttcgatc ggatttagct tttctttcgg agctgaatga tcgatttgac
    21601 gcgtcaattc cttgtaagca ggagccaatc cctgatccat gattatttct gcatatctat
    21661 cgagtaaaat gaaatactta atggaaggga tttgctgttt taatttttct gcattggcta
    21721 ctggctgttt tttcaatttg atacgaagcc gagtcgcaca tggaaggaca gaatcaatat
    21781 tgtcttttcc tcccaactgt tcaatcagca tatgaatctg ctcggacagt accatctgtt
    21841 caggattcat tgtcgtttcc attagaagac cactccactc actaatataa tattggcgta
    21901 aggtgtcatt tcgcctgtac gaataaatgc atgacagtta ttcaactctt ttttcatttc
    21961 ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat aatttgattt tttccaatat
    22021 ttccgggttc attgtcttta tttcttccgc caattcaatc gattgtacct caagttcttc
    22081 taaaacgttt tctaaaacag ataaaaaact tggaagaccg tttgagacag ccaaatctat
    22141 tttttcagta ccaaagggga caggcatgcc tgcatccccg atacttaatt tatcaaagtg
    22201 ccccatttgt gcgattactc gtgaaatatc tgaattgatt acttttcctt ttttcatgtt
    22261 tgtttcttcc tctcattgtt aattttagac tgctgattgt tctaaagctt cgcggtaagg
    22321 gatagaaggt tgcgcaccat aagactgcac agcaacagaa gaggctagat ttccataaag
    22381 gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa
    22441 tgtatcacct gcggctgttg tgtcaacggc atccacttta aaagcaggga cgataccgtg
    22501 ttctttttct gtatgataga aagcgccggc actgcctagt gtaatgatta ctgttccaat
    22561 acctaattca tggagttttt cggctgctgc gaccaaactg ttatgatctg tcactctgat
    22621 tcctgtaatg atttctgttt ctgtttcatt tggtataatg atatctgtct tttcaagtag
    22681 ttcagtagga atcgtttcac gagctggtgc aggattcagg atcgttgttt ttcctgcgtc
    22741 ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact gagcaataat
    22801 aaagtcactg tttgtaatca gttgcttgtt ttttaggact tcttgttcat ggaaagcatt
    22861 atttgcccct gcatggatca agatactatt ttctcctgct gcgtctacca taatcattgc
    22921 ttgtccggtt gtggcgcctt ggatttcagc tatcccagat gtatcgatgt tttcttttgt
    22981 cagtagatct aataactgtt gtccttcgct gtcagcccca acgcctccaa taaaagatgt
    23041 ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca cctttacctc cgccagcgtg
    23101 aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta aacgaagggt
    23161 agtgtccata ttaatacttc caattactgt aaccgtgttc attttacaaa cctccaacta
    23221 tctcatttat tttgatggga aaattatttt ttaaaacgtt ttagtaaaac gttttaatag
    23281 ttcgataaaa atatagcaca ccttgaaagc tatttcaagg gtgcagttga aaatcttttt
    23341 tctaatcgta caggtaatct tttttcttcc cattcttttt ctgggaattg gatccgatct
    23401 aacaataact tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt
    23461 ttcggcttga tatactcgca catatctata ttatcatagc caataatgct gtagtcttca
    23521 gggatgcttt ttccagcttc ctctaatccg cgatataatc caaaagctaa ttcatcattt
    23581 aaagcaaaca caccagttgc agcagaatca agaagttctg ctgtgatctg gtagccacct
    23641 tgttttgaga actgtgtagg taagaggatc agttggtcgt gagggatttg gtaaacatca
    23701 agagcagatt taaacccctc aatccgagcg tggacatttt ccggtggatt ttcaggatag
    23761 acaagggcga tcgtttgatg tcctagacta agcaagtgca tgcccgctag gtaaccaccg
    23821 cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag attttttctg atccaaaaca
    23881 atgaagggcc ggccttgttt tttcaaattt tcgttgattg catctgtaga gacagcagaa
    23941 gtcgcgataa tgaatccatc cacacctcga cgtgtgagct cggctagata ttcgatttct
    24001 ttttgatgat cagaatcagc attacataat atcgtgacga aattctgctt atacaagata
    24061 tcctcaattc ctcgcatcag tgtactgaag aatggattcg tgatatcagg aacgagtaca
    24121 ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct
    24181 aattcttctt tggcagctaa aactttctga acagttttcg gactgaattt ctccgtattt
    24241 ccatttaata tcaaggagac agttgtgatt gaaacgccgg aaagtttagc gacttctttg
    24301 attgtgatct tttttttcac catgttcata ctcctatcta aatcctgtgg ttattttccc
    24361 atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag tacttatttg ctttgttgga
    24421 tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat atctttttta
    24481 tgaacaattg atgggattct ttgtcacgat cactgcaagc atcttcagca agcacgatat
    24541 ggtacccgtg ttgaaaggca tctaatgcag tagcatatac accgtttgtt gtcgctacac
    24601 cgcttaaaat gatagtttcg attccacgcc ggcgaagttg aagatcaaga gaagtaccaa
    24661 aaaaagcact tggattgtat tttgtgatag tcaacatatt atctgtatct ttcaaagaat
    24721 ccttcaaaag taaatcagtg aattctggag gtacagaagc agtcattcca cccatatcag
    24781 tttgttgact caaataaggg aaagtggaga tgtctacgtt cactaaaact ttcaaggcag
    24841 cggtacgttt caatgttctt actaacaaat cattgtttgt cagaatagat gctgcagtgt
    24901 gtggagagag tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg acaaaagcag
    24961 tttttgataa atcaaaaatc atattttcct ttaaatcatt catgatgttc cttctttcaa
    25021 ataacaatgt tttttatgta agtaaatcat aaatcgttta tttgtcaact aaattaaagg
    25081 attcctttct ttagtctaga gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat
    25141 tcgatgcata gatatagcga gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc
    25201 gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa cgaccattac agcaaatgac
    25261 gatatttctt tttcaattga aaaaggcgaa ttagtgatta tacttggtcc aagtggagca
    25321 ggaaagtcaa cgattcttaa tattttaggt gggatggata cacctgatga aggtcagatc
    25381 attattgatg acacagatat tgcgcaattt tcagataaac agcttactgc ttaccgacga
    25441 acagatgtag ggttcgtttt ccaattctac aatctcgttc ctaatctaac agcaaaagaa
    25501 aatgtggaat tggcaacgga agtttctcct gatgcgcttg atcctgtaga ggtattgagg
    25561 caagtgggcc tagctcatcg attgaataac tttccgtccc aattatcagg aggcgaacag
    25621 cagagggttt caattgctcg tgcactagct aaaaatccca aacttctttt atgtgatgaa
    25681 ccgacaggcg cgctggattt tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct
    25741 agcagacaac acggcaatac cgttttgatc attacacata attctgcttt agcgcccatc
    25801 gctgatagag tcattcacat taatgatgct aaagtaaggt ctgttgaatt aaatgatcat
    25861 ccttcatcca ttgatgagat tgtatggtag ggggatttta tgaaaacaag aacatatctt
    25921 aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat catccttatt
    25981 gtcatgctag gaacactttt atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa
    26041 acgatggatc actatgttgg tacagcagga ctttctgatt tgcaaatcgt ttctactggt
    26101 ggcttgacac aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca
    26161 ggaaaacaga tctattattc caatactggc aaaaacgaag tgattcaaat attttcttat
    26221 aacaaaaata acaagcagaa caagctacaa ttaactgatg gaaaactccc ggaaaaacca
    26281 aaccagcttg tattggatga aaaagcggaa gatgaagggt ataaaatcgg tgatatttat
    26341 cagattgatt cagatgaatt gaaagaaaaa gagtatacca tcacgggctt tgtccgttct
    26401 ccattgttca tcaacaattt ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat
    26461 tattttgtct atttacctga atccgttttt aaaacagata tttattctgt cttatatctt
    26521 gattttacaa acgtaaagaa tttgaatacg tacagcaaag cttacaaaga taaaatggag
    26581 caaaatcaag aaaaggcaga aaagtattta caagatcgac ccgaagaacg cttagaagag
    26641 ctgaaaaaat cggccaacga agaattagaa ccggcacagc aaaaaatagc ggatggcaaa
    26701 gcacaaacaa cgcaagcaag aacacggtta gaagatgcta aaaaacagtt agcgcaacag
    26761 tcagcaacta tccagcagct tcctgaagcg caacgaacaa tcgctgttca aacattatct
    26821 caacaagaag ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga acgtgaactg
    26881 gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca
    26941 tcatacctat acaatgaacg ttcagaaaat gtcggttttc aagaatttgg tgatctgtct
    27001 gatcgtatcg ctgcgattgc caatgttttt cctgttttct ttttctttat tgccgcattg
    27061 atcaccttta cgacaatgac acgtatggta gaagaaaacc gcagggaaat cgggacatta
    27121 aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta
    27181 gcctcaggga taggtattgt tttaggaacg atattaggaa cgaatctttt gccaaggatc
    27241 atttttgagc tgtctaacga gcggtatgat attgggagtg cgcttatttt ttatgactgg
    27301 ccgccgattt tacaagcagc tgccgcattc ttcattgccg catttggcgc tgcaatgatc
    27361 gttttattca aagatttaag agaacggcct gctgcattgc ttcaaccgaa agcaccgaag
    27421 ccagggaaac gcatttttct tgaatacatc actccgctgt ggtcacgcct tagctttaat
    27481 caaaaaatca gttaccgaaa tctcttccga tataagtcaa gaatgcttat ggcgattatc
    27541 ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt
    27601 tctgtttcag ccaaacaatt tggaccgatc attgactatc aagcaatcgt tacattggat
    27661 tcatccacgg aagatagtcg tcaaaaagca gcggatatgc tgcagaagca agaaaaagta
    27721 aatgagatcc tagctattca tactcaaacc gtagaattga gaaaaaaagg acatgcttcg
    27781 caaagtctga cagtgatcgt accaagtaaa acagctgatt tggatcgttt tatccattta
    27841 actgatcttg aagagaaccc acgctccttg cctaaaacag gaatcgctat tacgcaaaaa
    27901 gtagcagaac tatttgactt atctacagga gatcatattt cgctttatga tgaagatcaa
    27961 aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag ctttatttat
    28021 atgtcccctg aatattatgc ttcagttact gatcaatcga tgatggataa tgcatttttg
    28081 ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc
    28141 acagacgctg tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca agaagaatca
    28201 atggcgaact tagactcggt tgtcttaatc tttgtcgtat tatcaggact gctggctttt
    28261 atcgtcttgt ataatctaac taatatcaat atttccgagc gtgtacgcga attatctaca
    28321 ataaaagtcc ttggattttt tgataaagaa gtcacgatgt acattgttcg agaaaatatc
    28381 atctttactc ttttaggaat catcggagga tttggtgtag gatatatatt gactgacttc
    28441 atcttgcaac aagcgtctat ggagaatgtc atctttccat tagtgatcac atggtgggct
    28501 tatgtcttat cagcaggatt aactatcgta tttacggtta ttgtgatgat tgttacccat
    28561 ttcaaattaa agcatatcga tatgattgat gcgttgaaat cgaatgaata aaaacagagg
    28621 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggcaaaat agtttttcat
    28681 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccat gaatcagagg
    28741 ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat
    28801 gttttacatt tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa
    28861 gaaacaagaa tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt
    28921 gttttttatt gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc
    28981 ggttcagtta taataataaa tgataacttg tatatataag ttgtggtagg taataaatca
    29041 gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc
    29101 aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa
    29161 ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc
    29221 cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt
    29281 acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg
    29341 ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca
    29401 ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca
    29461 aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta
    29521 tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc
    29581 aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat
    29641 gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat
    29701 acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg
    29761 gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg
    29821 tatacagaac ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc
    29881 gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga attaaagcga
    29941 ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt
    30001 aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa
    30061 caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat
    30121 atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac
    30181 gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg
    30241 tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac
    30301 acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa
    30361 gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa
    30421 ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa
    30481 gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta
    30541 ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc
    30601 aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca
    30661 taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag
    30721 ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa
    30781 gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg
    30841 aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc
    30901 acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc
    30961 tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca
    31021 aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa
    31081 cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa
    31141 atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct
    31201 ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca
    31261 atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac
    31321 tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa
    31381 gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc
    31441 caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga agcgaaaaat
    31501 tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa
    31561 tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga
    31621 ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact
    31681 gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa aagataatac
    31741 aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg
    31801 aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct
    31861 aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt
    31921 tcttttttca ctctttttcc tcctttatct ataaattcat tatagacaaa agcgggtata
    31981 aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc
    32041 atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg
    32101 ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa
    32161 gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt
    32221 agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt
    32281 gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga
    32341 aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc
    32401 taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga
    32461 aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc
    32521 gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca
    32581 cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc
    32641 aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga
    32701 agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta
    32761 tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa
    32821 ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga
    32881 agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa
    32941 ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc
    33001 aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa
    33061 tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga
    33121 gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg
    33181 ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca
    33241 aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa
    33301 attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac
    33361 agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta agaatggaac
    33421 aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa
    33481 ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat
    33541 tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg
    33601 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
    33661 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
    33721 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
    33781 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
    33841 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
    33901 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
    33961 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
    34021 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
    34081 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
    34141 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
    34201 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
    34261 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
    34321 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
    34381 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
    34441 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
    34501 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
    34561 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
    34621 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
    34681 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
    34741 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
    34801 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
    34861 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
    34921 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
    34981 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
    35041 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
    35101 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
    35161 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
    35221 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
    35281 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
    35341 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
    35401 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
    35461 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct
    35521 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
    35581 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
    35641 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
    35701 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
    35761 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
    35821 accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag
    35881 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
    35941 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
    36001 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
    36061 aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
    36121 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
    36181 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
    36241 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
    36301 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
    36361 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
    36421 ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct
    36481 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
    36541 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
    36601 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
    36661 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
    36721 gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt
    36781 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
    36841 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
    36901 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
    36961 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
    37021 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
    37081 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
    37141 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
    37201 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
    37261 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
    37321 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
    37381 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
    37441 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
    37501 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
    37561 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
    37621 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
    37681 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
    37741 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
    37801 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
    37861 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
    37921 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
    37981 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
    38041 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
    38101 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
    38161 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
    38221 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
    38281 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
    38341 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
    38401 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
    38461 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
    38521 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
    38581 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
    38641 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
    38701 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
    38761 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
    38821 cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta aaatcatatc
    38881 gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag acaacgaaac
    38941 tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc
    39001 agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt
    39061 ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg
    39121 cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa
    39181 acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa
    39241 aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg
    39301 gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg
    39361 aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag
    39421 tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt
    39481 ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag
    39541 gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta
    39601 aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact
    39661 attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg
    39721 attccattga taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg
    39781 aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc
    39841 tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc
    39901 tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga
    39961 caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat
    40021 tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc
    40081 ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga
    40141 tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag
    40201 gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac
    40261 aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat
    40321 cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac
    40381 tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc
    40441 aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc
    40501 aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact
    40561 tttctcctaa atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt
    40621 taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc
    40681 atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa
    40741 tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa
    40801 gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga
    40861 ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag
    40921 gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg
    40981 gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca gtaacgacga
    41041 tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc
    41101 tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa
    41161 ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt
    41221 ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct
    41281 tattttcgaa gaagctattt ctggaacaca gtttattcgg aaaagggagg tgtgacaaga
    41341 attttgtcac acctccctta tttattttga aaatgagaat actattatca aaatagagaa
    41401 tatatagaaa aattctcatt attcaaacgt aaataatcag ttattttgat tttttttata
    41461 ttttattagc taataattgg tattttaaaa gaaattactg tagaattgga ttataattaa
    41521 aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag
    41581 caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac
    41641 cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata
    41701 ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt
    41761 taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga
    41821 ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc
    41881 gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt
    41941 catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt
    42001 ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa
    42061 gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca
    42121 gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt
    42181 gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct
    42241 ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt
    42301 atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt
    42361 aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta
    42421 ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt
    42481 aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc
    42541 aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga
    42601 catgataaga taactgagtt gttaaaaaag aaccacttgt gaagcttgat tctatcgaaa
    42661 aaaacttcaa aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg
    42721 taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct
    42781 ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcgggaaa cactcctgat
    42841 gaagtagacc tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat
    42901 tcaattcaag acaacaaaca
//
LOCUS       DACEJZ010000012        40677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEJZ010000012 DACEJZ010000000
VERSION     DACEJZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.012"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..963)
                     /locus_tag="IW656_000626"
     CDS             complement(7..963)
                     /locus_tag="IW656_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0836354.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IW656_000627"
     CDS             1137..1697
                     /locus_tag="IW656_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836355.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="IW656_000628"
     CDS             1690..2514
                     /locus_tag="IW656_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0836356.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="IW656_000629"
     CDS             2524..3141
                     /locus_tag="IW656_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0836357.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..3842
                     /locus_tag="IW656_000630"
     CDS             3159..3842
                     /locus_tag="IW656_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0836358.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4525..5808
                     /locus_tag="IW656_000631"
     CDS             4525..5808
                     /locus_tag="IW656_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0836359.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELVIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5824..6711
                     /locus_tag="IW656_000632"
     CDS             5824..6711
                     /locus_tag="IW656_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836360.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6728..7564
                     /locus_tag="IW656_000633"
     CDS             6728..7564
                     /locus_tag="IW656_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0836361.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7567..8190
                     /locus_tag="IW656_000634"
     CDS             7567..8190
                     /locus_tag="IW656_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836362.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8187..9479
                     /locus_tag="IW656_000635"
     CDS             8187..9479
                     /locus_tag="IW656_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0836363.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9838..9935
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10051..10632
                     /locus_tag="IW656_000636"
     CDS             10051..10632
                     /locus_tag="IW656_000636"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0836364.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10635..11942
                     /locus_tag="IW656_000637"
     CDS             10635..11942
                     /locus_tag="IW656_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0836365.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12358..12846
                     /gene="purE"
                     /locus_tag="IW656_000638"
     CDS             12358..12846
                     /gene="purE"
                     /locus_tag="IW656_000638"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0836366.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12830..13963
                     /gene="purK"
                     /locus_tag="IW656_000639"
     CDS             12830..13963
                     /gene="purK"
                     /locus_tag="IW656_000639"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0836367.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13976..15271
                     /gene="purB"
                     /locus_tag="IW656_000640"
     CDS             13976..15271
                     /gene="purB"
                     /locus_tag="IW656_000640"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0836368.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15451..15828)
                     /locus_tag="IW656_000641"
     CDS             complement(15451..15828)
                     /locus_tag="IW656_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836369.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            16084..16812
                     /locus_tag="IW656_000642"
     CDS             16084..16812
                     /locus_tag="IW656_000642"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0836370.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16812..17066
                     /gene="purS"
                     /locus_tag="IW656_000643"
     CDS             16812..17066
                     /gene="purS"
                     /locus_tag="IW656_000643"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0836371.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            17068..17739
                     /gene="purQ"
                     /locus_tag="IW656_000644"
     CDS             17068..17739
                     /gene="purQ"
                     /locus_tag="IW656_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0836372.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17740..19962
                     /gene="purL"
                     /locus_tag="IW656_000645"
     CDS             17740..19962
                     /gene="purL"
                     /locus_tag="IW656_000645"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0836373.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19947..21386
                     /locus_tag="IW656_000646"
     CDS             19947..21386
                     /locus_tag="IW656_000646"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0836374.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21418..22461
                     /locus_tag="IW656_000647"
     CDS             21418..22461
                     /locus_tag="IW656_000647"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0836375.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22458..23036
                     /gene="purN"
                     /locus_tag="IW656_000648"
     CDS             22458..23036
                     /gene="purN"
                     /locus_tag="IW656_000648"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0836376.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            23033..24568
                     /gene="purH"
                     /locus_tag="IW656_000649"
     CDS             23033..24568
                     /gene="purH"
                     /locus_tag="IW656_000649"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0836377.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24584..25858
                     /gene="purD"
                     /locus_tag="IW656_000650"
     CDS             24584..25858
                     /gene="purD"
                     /locus_tag="IW656_000650"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0836378.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25980..26894
                     /locus_tag="IW656_000651"
     CDS             25980..26894
                     /locus_tag="IW656_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0836379.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(27027..28592)
                     /locus_tag="IW656_000652"
     CDS             complement(27027..28592)
                     /locus_tag="IW656_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0836380.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28585..30036)
                     /locus_tag="IW656_000653"
     CDS             complement(28585..30036)
                     /locus_tag="IW656_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0836381.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(30050..31066)
                     /locus_tag="IW656_000654"
     CDS             complement(30050..31066)
                     /locus_tag="IW656_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836382.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31286..32374
                     /locus_tag="IW656_000655"
     CDS             31286..32374
                     /locus_tag="IW656_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836383.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32428..33954
                     /locus_tag="IW656_000656"
     CDS             32428..33954
                     /locus_tag="IW656_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836384.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33956..35161
                     /locus_tag="IW656_000657"
     CDS             33956..35161
                     /locus_tag="IW656_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836385.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35418..36395
                     /gene="guaC"
                     /locus_tag="IW656_000658"
     CDS             35418..36395
                     /gene="guaC"
                     /locus_tag="IW656_000658"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0836386.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36659..38083
                     /locus_tag="IW656_000659"
     CDS             36659..38083
                     /locus_tag="IW656_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0836387.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38544..39653
                     /gene="pdhA"
                     /locus_tag="IW656_000660"
     CDS             38544..39653
                     /gene="pdhA"
                     /locus_tag="IW656_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0836388.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39656..40633
                     /locus_tag="IW656_000661"
     CDS             39656..40633
                     /locus_tag="IW656_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0836389.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        12987 a         6937 c         9110 g        11643 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg
     3781 gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat
     3841 aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac
     3901 tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta
     3961 atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct
     4021 tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg
     4081 acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa
     4141 gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt
     4201 actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct
     4261 atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca
     4321 tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct
     4381 tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc
     4441 ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata
     4501 acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt
     4561 gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac
     4621 atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat
     4681 gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac
     4741 acaaaattag gtacactagc agcatcagat gatatctggg acattttcca actgaacaat
     4801 caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata
     4861 attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta
     4921 gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa
     4981 caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc
     5041 tatcaaatca ctgaagaatt agtaatctat gctatttcaa gattcgaaga ttttatagct
     5101 ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat
     5161 ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt
     5221 aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg
     5281 gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac
     5341 cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca
     5401 cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt
     5461 atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac
     5521 agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt
     5581 cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa
     5641 atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa
     5701 attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa
     5761 gaagcagcag agagtcttta tgagtttgcg tcaaacattc taggatagca gcaggaggaa
     5821 ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag acaatgtggt cggttacgta
     5881 tttgcttctc cattcatcct aggatttcta ttgataacag tgataccgat gatcttgtca
     5941 ctttattatt cgttcaccga ttatcgtttg ggaaatccag tctcatggat cggcttggat
     6001 aattatataa agatgctgag tgatgcccgt tttttgaact ccatccgagt cacttttctc
     6061 tatgtatttg catctgtacc tgtgaaactg atctttggtc tattgattgc ttatctttta
     6121 acgaggaata tcaaaggaac gactttcttt cgttctttgt attatgtgcc tagtttgatc
     6181 ggtggcagta tttctatcgc tctagtttgg cgggagttgt tttctaaaaa cgggttagtg
     6241 aatattttcc tggaacgatt aggcgcacct gcgctttcct ggtttagcga tcaaaaactg
     6301 gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg gttcggcaat gatcatcttt
     6361 gctgcaggta tcaaagagat cccaggctcg tattatgaag cggcagagat cgatggagcg
     6421 aacaaatggc agacgtttta caagatcacg ttacctcttc tttcgccgat cattttgtat
     6481 aacttggtta tgcagacaat cgcggcgttt atgactttta cgcaggcatt tatcattacc
     6541 caaggtggtc cgaatgatgc cacgaacttt tatgctcttt atgtgtataa ccaagctttt
     6601 tcttttaatt ctatggggta tgctagtgct atttcgtgga tcatgctgtt gattatggga
     6661 gctgtcacct ttctgatttt caaatcatca aaaaaatggg tcgttgtgtg aatgagagga
     6721 ggaagaaatg aataatacag taacgaaatg gtttcaatac ggttttattt tgctattagg
     6781 tctagtcatg atttatccgg tcttatggat gatcgcaggt tcttttaaaa cggatcaaga
     6841 gattatgagt ggttcgctga atttgattcc agagactttt caatgggaaa attacgcaaa
     6901 aggatgggcg gggtttgctg gaatttcatt ttttactttc tttaggaatt cgtttgtcat
     6961 ctctgcagtt tcgacgatag ggacagttct gagctcgact tgtattgctt acgcattatc
     7021 gagaatcgat ttcaaaggaa aaagattctg gttcgtcgtc atgttagtaa cgatgatgat
     7081 tcctgcacaa gtgattttga ttccgcagtt tattatttac aatcggctga atttggttgg
     7141 gacgtatgtg ccgctgattt taccacattt ttttggacaa gcatttttca tttaccagat
     7201 catgcaattc atggtcagca tccccaaaga gttagatgaa tcagcgatta ttgacggatg
     7261 cagcaagtat agtgtcttta ccaaaattat ttttcctttg ctgaaacctt cgatcatcac
     7321 gacgatcatc atccagtttt attggaaatg ggacgacttc atgggaccat tgatttattt
     7381 aaacaagccg agatcttaca cggtatcgat tgccatcaaa ttatttgcag acgctggatc
     7441 tagtacgact tacgcgagta tgtttgcgat gtctacgttg tcgctgctac cggtttttct
     7501 gattttctta ttcttcaata aatatcttgt ccaggggatc agtacaagcg gtctgaaggg
     7561 ctgaatatga aaaagccaat agaagagatg tttctttaca agctcaacta ctatgtctgt
     7621 cggatttttt taaccagtct tatcagtaat gctcttttcg ctttagttct aggatgtttg
     7681 ttcactttag gattagtgct gtctgctcgt gttgtctatc tgatttattt tttgatgatg
     7741 tatccttctt attatctatc tgtccattac ttggcagaaa aaaaggtatg ttggaggaaa
     7801 ggaaaaatca aacggatgtg tttcatcatt acgttgtttc agctgtttgt cattattttt
     7861 gcagttgatt ggtatttatt agtgggaaag attggagctt ggtatctgat tccttttcta
     7921 ttgtgtctgt tgctttttgt atcccatagt ttttttagta cgctaattga tatctgcaaa
     7981 gaagataaag cagatagtct tgtttatttg atcaaaagga atatctggct gttgtcaaaa
     8041 aacgtcaagg ggtttctgtg gtatttatgc cttttgtatg gactgttctg gtgtatacag
     8101 catgattttt tactcactgc attactgtat ccagggctgc catttgtcac agtattttgc
     8161 ttaaaagaaa aggagaatga ctttaaatga agaaaaaata tgtacaagtc ggacttggtg
     8221 ggagagcgcg ttttttttat gaggcaattg cctctacgta tcatcaaact tgtgaactag
     8281 tcggtttttg tgatgtatct caaacaagga tggattatgc aaatcggata cttgtgaaca
     8341 agtatggaca gaaaaaagta aagacatgta tgccatcaca atttgagcaa ctactggatg
     8401 acgtacggcc ggctgaagtc attattacgt cagtagatcg aacgcatcat gattatatca
     8461 ttcgtgcaat gaaaaaagga tatgatgtta tttgcgaaaa acctctgact atcgatgagg
     8521 aaaaagctca agaaataata acggtccaaa aacagacggg aagaagcctg cgagtcactt
     8581 ttaattatcg ttatgcaccg tatcacacga agataaagga actgatagaa agtgatgtga
     8641 ttggtgatgt gttttctatc cacttcgaat ggctgttaaa tacgcaacac ggggcagatt
     8701 atttcagaag atggcatcga aataagcaca atagcggcgg gcttcttgtt cataaatcta
     8761 cccatcattt tgatctagtg aatttttggt tgaacagccg tccccaaaag gtatttgcat
     8821 tcggtggatt aaacttttat gggatgcata atgcggagaa acgcggagta gaaatatttt
     8881 acagtcgatg ctatggaagc gaactagcta aagctgatcc atttgctata gacctgtcag
     8941 ccaatgagga attgcgagaa atgtatttag aagcagaaaa agacagtgga tatattcgtg
     9001 accgcagtgt attttccgat gatatctcta ttgaagatac actaggcgta acggtcaaat
     9061 atgaaactgg tgcgattttg acgtattcgc taaacgcgta tatgccgtgg gaaggattca
     9121 atgtttcttt caatggcagc aaaggacgga tcgaaatgaa agtgatcgaa aagtcttaca
     9181 tcaatgcagg tggagataaa aaggatgaag gagcagccgt tcttcagtcc atccatgtct
     9241 atccgatgtt cggtgagcca tatgaggtaa tcgtcgaaca agaagcgggc ggacatggcg
     9301 gaggagatcc gcgtttatta gatgatctat ttggtgaaga aaaagaagaa gatccgtgga
     9361 atcgggcagc aactcacgta gatggtattt tgtcgatttt gacaggaatc gcagcaaatc
     9421 attctattgc ttcaggaaaa gcagtagtga ttgacgagct tgtcagctta aaagaataag
     9481 aatagattta cggaaattac ttcccgtagt ttataaaatc ttgccttatt gccgataggc
     9541 tgtcttttga acactattta ttcggaaaaa ggggctatga catgcttttt gtcatgcctt
     9601 ctttttatgt gggctgcttt tttagatagg agaaaataaa aataactatt taaacccgaa
     9661 caaaaaggaa taatgcatta aaaatcatta gattttacaa aaaaataagt aaaccacgtt
     9721 gtcagtttca gaaagtagtg ttagactgtt ttcaatattt gaaacaacat tttttattca
     9781 aatatggcaa actgccttca cgtgcaaaac agttgcaaga aaatgaattt acactattat
     9841 tagttttaca tacctactta tatatcgtca taatatggat gacagtttct agccagtacc
     9901 gtaaatgctg gactataagt aaaagattgg ctatttcacc cttcttttct tatgataaag
     9961 tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc ggttatttcg
    10021 gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg tattcaaaaa
    10081 gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac tcaccaagta
    10141 gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa agaacaagga
    10201 atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt tgctgcaaga
    10261 gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat ggatgaagaa
    10321 ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat ttctatttct
    10381 cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt ggcaaacgga
    10441 caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt agaaggaatc
    10501 gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga aatgggattg
    10561 agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga gtttcttgag
    10621 gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt ttagggctgc
    10681 agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc ggtacaggac
    10741 taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt atgtgtggat
    10801 tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg cctgtcgttt
    10861 taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat gaaggagtag
    10921 gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt tctggtgtct
    10981 tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc accgttattg
    11041 gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca acagcagctg
    11101 attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta attgtaggcg
    11161 tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt ttagtcggag
    11221 gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga gatgcgccgt
    11281 tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta tggtcgattt
    11341 tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta tattttgcac
    11401 ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga tatcgtgcag
    11461 aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact ggcttttctc
    11521 aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc tatttctctg
    11581 cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca caaatcatcc
    11641 ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca gtccaaggaa
    11701 tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt gttgctgttt
    11761 ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa atgccgcaaa
    11821 cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct atcatattga
    11881 atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc ttcaagaagt
    11941 aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag agaaacaaac
    12001 ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg tcagctgatt
    12061 gaacaccgtt gaaggaagag gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa
    12121 tttcaaaaaa aagcttctca tgtttgttga atattgttcc ttgtgaccga ggagttggca
    12181 ggagaacacc gtttatccat aaaaaggaat gtgacctaac ttttgtcaca ttcctttttt
    12241 ttatctttaa taaacacgaa caataaaatg tttttctttg ttaacattcg aaatttcttt
    12301 gacatcaaaa aaagtgattg ttaaaatatg aatgtagaaa aactgggagg aaaaacaatg
    12361 acagcaaaaa tctctgtgat tatggggagt acttcagatt gggaaacaat gaaagaagca
    12421 tgtcaaatat tggatgaatt cggtgtgtct tatgaaaaaa aggtcgtttc tgctcatcga
    12481 accccagatt tgatgtttga atatgcacaa aacgctcgaa aaaaaggaat caaaatcatt
    12541 attgcaggag cagggggagc cgcgcatctg ccgggtatgg tcgcatcaaa aacaactttg
    12601 ccggttatcg gtgtaccggt ccaatcacga acattaaatg gtttggattc gcttctttcg
    12661 attgtccaaa tgcctggagg agtacctgta gctactacag caatcggaaa agcgggagca
    12721 attaatgctg gactacttgc gatacagatg ctttccatgt atgatttaga attggaggct
    12781 aaattggctg aaagacgttc aatgttggct aaaactgtta tagaaagtag tgatcaactt
    12841 ggttaaacct ttgttgcctg gttcaacgat cgggatcgtc ggtggcggtc aattaggtcg
    12901 tatgatggcg attagtgcaa aagaaatggg ctttaaagta ggtgtgctgg atcctgtgag
    12961 cgattgtcca gccgcacaag ttgcagattg gcacattgaa agtgtctatg atgacacctt
    13021 tgcgctgaaa gagcttgcca gaagaacaga tgtcattacc tatgaattcg aaaacgtcag
    13081 tatagaggcg ttaaatgcga ttttacccat gtcatttatt cctcaaggga cagacttgct
    13141 tgcgattacg caagaccgcc tgttagaaaa atcttttttg gaaacgaata acatcgtgat
    13201 tgctccttat gcaacgatcg tcagtccgac ggatatccaa gatgcgattg acgggatcgg
    13261 ctatccttgt gttttgaaaa ccactcgagg cggctatgat ggcaaggggc agtatgtttt
    13321 gaaaagtcgg gcggatctag ctcctgccat ggacctttta agagaaggaa cctgtgtttt
    13381 agaagcatgg atcccgtttg aaaaagagat ttcgatcatg gtggcaggaa atggtcaagg
    13441 agatttcacg acttttccgg tcgtggaaaa catccaccac aacaatattc ttcatgaaac
    13501 gattgctcca gccgcgattg atcaggatgt gatcgaggag gctgagcgta ttgcccgtgt
    13561 gattgcagaa gcagtgagcc tttcaggtgt tttaggggta gaaatgtttt taacaaaaac
    13621 aggcgggcta tatgtgaatg aattagctcc tcgtcctcac aattctggac attattctat
    13681 tgaagcttgt tctatgagtc agttcgatgc ccatattcga ggtatttgtg gttggccatt
    13741 aggagaaaaa gtacgtttga taagcaatgc agtaatggtc aatgttttgg gagaacaact
    13801 gctggaaagc tatcgcttga ttgagaataa gccagactgg caattccatt tctatggaaa
    13861 aaaacaagca aaaaaaggtc gcaagatggg acatatcact atcttgacag aagacatcca
    13921 gcaaacgctg gaagaaatca aacaaacgaa tatttgggat tagaggagaa aaaacatgtt
    13981 agaacgttat acacgccctg aaatggggaa tatctggacg gaagagaaca aataccgtgc
    14041 gtggctcgaa gtcgagattt tagcagatga agcttgggca gaacttggtg aaatcccgca
    14101 agaagatgtg aaaaaaattc gtgagcatgc atcatttgat atcgatcgga tcttggaaat
    14161 cgaaaaatta acacgccatg atgtcgttgc gtttacacga gcggtttctg aaacattggg
    14221 agaagaaaga aaatgggtcc attatggtct aacaagtacg gatgtcgtag atactgctta
    14281 tggctatctc atcaagcaag caaatgatat tttacgggaa gacttgaaac gttttacgga
    14341 aatcattggc gaaaaagcaa aagaacacaa atatacagtg atgatgggtc ggacacacgg
    14401 tgtccatgct gaaccaacta cttttggatt gaaacttgcc acatggtatt cagaaatgaa
    14461 acggaacatc gaacgatttg aacatgcggc aaaaggagta gaagcaggaa aaatctcagg
    14521 agcagtggga acgttcgcaa atatcccgcc atttattgaa gagtatgtat gtaaacaatt
    14581 ggggatccgt ccacaagaaa tttctactca agttctccct cgggatctac atgctgaata
    14641 tttctcagcg atggcgctta ttgctacaag tattgaacgt tttgcaacag agatacgcgg
    14701 attgcaaaaa tccgaaacac gtgaagtaga agaatttttt gcgaaaggtc aaaaaggttc
    14761 ttctgctatg ccgcacaaaa ggaatccgat cggttcagaa aatatggctg gtctagccag
    14821 agtggtgcgc ggacacatgg tcactgccta tgaaaatgtc acattatggc atgaaagaga
    14881 tatttctcat tcttcagcag aacgaatcat cttgcctgat accacgattt tattgaatta
    14941 catgttaaat cgttttggca atatcgttaa aaatctaacg gtctttcctg aaaacatgaa
    15001 gcggaatatg tctgcaacat ttggtttgat ttatagccag cgcgttatgc tgaaattgat
    15061 cgataaggga atgacaagag aagaagctta tgatcttgtt cagccaaaaa ctgcgtacgc
    15121 ttgggacaac caagtgcagt tccgcccgct gttagaagaa gatccaacca tcacatcggt
    15181 attatctaaa gaagaaatag acgatgcctt tgattatcat catcatttga aaaacgttga
    15241 cgtgatattc aaacgagtag gtttggaata aagaagagtg agttgcaaaa ttcaattaaa
    15301 atagaatagc aaaaataacc aaaaaagaag cagatccgtt agcggttctg cttcttttag
    15361 tttattatat tttcttatca ataataggaa cctgatttta aaagcggcta cctctttacc
    15421 gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa atggcttggc tattataact
    15481 aaaatgctgg ttatggagat gttgcagcaa gttttcgaga aaagcgtatt ccatctcttg
    15541 tccattgaat aagatctgga tacgataatg atttttgaaa cgcttctgtt catgaatctg
    15601 actgattgaa agttcgcctt tgtgttctgt ctgataaaga aaagtccttt tacggcattc
    15661 agcacagcgc ttggttgctc ggcggatttt tcggaatgta taaaaactag tatctttatt
    15721 gcataaataa acgtctttga tcgacagcaa atcattatat agacaggaac gttcttcagc
    15781 tgttaattct gataaaaaaa gctgaccttg ttttattgca tcttccatcg gcaagtgcct
    15841 cctcgaaccc attatccaaa tttttagtta attctaatat accatcatta agtataaatg
    15901 taaacgcttg ttgatggtat aagattttcg tttctgataa aacacgaaca ttaaaagaat
    15961 ctaatgcctt aaatgtacat atttttgttg atttataaaa agatgattgc taaaatttaa
    16021 agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat gtaaataaaa aggagagacg
    16081 tacatggaga agaaagaact tttgtatgaa ggaaaagcca aaaaaatata tcttacttct
    16141 gatgagaact tgatttggat agaatattta gatcaagcaa ctgcgttgaa tggcgcgagg
    16201 aaagaccaga taagtggaaa aggagaattg aataatcaaa tcacaagtgc aattttccgt
    16261 tatctgactc aaagaggaat aaaaaatcat tttattgaac aattgtctaa aaccgaacaa
    16321 ttagcaaaaa aagtacaaat tatccctctg gaagtggtta tccgaaatat tgcagcagga
    16381 agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat tcatcaaacc aatcattgaa
    16441 ttctactata aggatgacca gttggatgat ccatttatca atgaagagca tatctatttc
    16501 ttacaacttg caaagccgga agaaatcagt gagctgaaag cccaagcgtt ggctattaat
    16561 gaaattttga ccgaattatt tgctaaggtc ggtttggttt tagtagattt caagctagaa
    16621 tttggccggt cgaatggaca aattttgctt gcggatgaag taacaccgga tacttgccga
    16681 ttatgggatg aacgtacgaa tgaacatatg gacaaagacg tttaccgcag agatctaggt
    16741 gatttgatcc cagtctatca agaagtatat aaccgtctat cggaactaac tgaagaaaag
    16801 gagcgagtat gatgtatttc gtaaaagtat atgtaaccta taaagattca gtgttagatc
    16861 ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg atatgaaaca atcgaagata
    16921 tccgaatagg aaaatacttt gagatacaac ttgctgatgc agaagaacca atcgatacag
    16981 tgattgaaac gatttgtgac aagctgcttg caaatgtaaa tatggaaaca taccgatatg
    17041 aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg tcatcgtttt tccaggttca
    17101 aattgtgacg cagatatgtt atgggcagtt cgcgagatca tgggcgctga tgccgaattt
    17161 gtacggcatg atgagtcctc tttagaagga tttgatgggg ttttactgcc gggaggattc
    17221 tcctatggtg actatttgag atgcggtgcg attgctcggt tttctacaat catggaagaa
    17281 gtgattcgtt ttgctgaaga agggaaacca gtattcggta catgcaatgg attccagata
    17341 ttgacagaag caggactttt gccaggtgta ctgagacgaa atgagtcttt gcattttatt
    17401 tgcaaaactg taccattgaa agtcaacccc aagacggcct ttacttcaga gtatgaagcc
    17461 gacgaaataa tctatcttcc agttgctcat ggggaaggaa attatttctg tgacgaagaa
    17521 acacttcaag aactaaaaaa aaataatcag attgccttta cttataatga caacatcaac
    17581 ggcagcgtgg aaaacattgc agggattacg aacaaagcag ggaatgtgtt aggaatgatg
    17641 cctcatccag aacgtgcagt agaagacttg ctaggatcag aagatgggaa acggttcttt
    17701 gcttccattt taaaaaactt cggaaaggtg accgtataga tgaaaacatt gaaagaaccg
    17761 acagctgaag agatcaagaa aaaaagaatc tatgccgaat ggggattgac tgatgaagaa
    17821 tatcgaatga tcgaagaaga tatcttagga agaatgccta attatacgga aactggactg
    17881 ttttctgtta tgtggagcga acattgttcc tataaaaatt ctaaaccggt tctacgcaaa
    17941 tttcctacta gtggacctca tgtgttgcaa ggccctggag aaggcgcagg catcgtcgac
    18001 atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc acaatcatcc ttcagccgtg
    18061 gagccttatg aaggagctgc gacaggagtt ggcggaatca tcagagatat tttcagcatg
    18121 ggggctcgtc caattgcgtt gctagattca ttgaggttcg gcgaattaga taatccaaga
    18181 acgaaatatt tactagaaga agtagttgca ggtatcggcg gatacggcaa ttgtatcggc
    18241 ataccgactg taggcggcga aattgcattt gatccttgtt atgaaggaaa cccattagtc
    18301 aatgcaatgt gcgtaggttt gattgaacac aaacatatcc agaaagggca agccgctggc
    18361 gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc gcgacggtat ccacggagca
    18421 acttttgcct cagaagaatt taatcaagaa gaagaacagc aacgttctgc tgtacaagta
    18481 ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt tggaattaat cttgcatcat
    18541 tcggattctc taataggtat acaggatatg ggagcagcag gacttgtttc atctagtgcg
    18601 gaaatggctt caaaagctgg ctcaggactt atattgacat tagatgaagt cccacaaaga
    18661 gaaactggga tgacacctta tgaaatgatg ttatcggaat cacaagaacg gatgttgatt
    18721 tgtgttaaat caggagagga agaaaaaatc caagaactat ttcagaagta tgatctagat
    18781 gcagtcacta tcggtaaagt aacagacgac gggcaatatc ggctatatca tcaaggagaa
    18841 gaagtagcaa atctgccagt cgatgcatta gctgaagatg caccagttta tcataaagaa
    18901 atgaaagaac cagctcgaat cgtagaattt cagcagttag ctccttatca gccagttatc
    18961 gaagaacctg gacaagtatt gcttgatctg ctgcagcaac cgacgattgc ttcaaaacgt
    19021 tcaatctacg aaacttatga ttcacaagta caaaccaata cagttgtccg tccaggaagc
    19081 gatgctgctg tgatgagggt tagagggacg aataaagcgt tagctatgac cacagactgc
    19141 aacgcgcgct atctgtattt ggatccaaaa atcggaggac aaatagcagt ggcagaagct
    19201 gctagaaata ttattgctag cggtggtaag cccttggcga tcaccgactg cctgaactat
    19261 ggttctcctg ataaaccaga agttttttgg gaattatcga cttcagctga cggaattgct
    19321 gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa atgtctcttt atataatgaa
    19381 acggatggtc aagcaatcta tccaacacca atgattggta tggtcggatt gattgaggat
    19441 cataaacata tcactactca ggaattcaaa aaatcagggg atctaatcta tatattaggt
    19501 aagacgtttg cagattttga tggcagtgaa ttacaaaaga tgcagcttgg aagaatcgaa
    19561 ggtgtgatcc gaaactttga tctatccatt gaaaagagaa accaagagtt agtattgaca
    19621 gccatccaaa acggattgat tgaaagtgct catgactgtt cagaaggtgg cttggcaatt
    19681 gcactggcag aatcagcttt caaacatcag ctagggatat ctgtacagtt tgaattgtcc
    19741 tctgctcaat tatttgcaga aactcaatca agatttgtgt tgacagtcgc accggaaaat
    19801 aagacacgat tcgaagaaat gatgggagat gctgctgttt tagcaggtaa ggtgacggat
    19861 gaagcgataa tcgaaatttc agcaactgac ggtcagatca aaatagaaac tgcagtagca
    19921 agaaagtgct gggaggatgc aatcgtatgt cttatgaagt gaagagttta aatgaagaat
    19981 gcgggatctt cggtgtttgg ggtcatccag acgccgctag tgtggcatac ttcgggctcc
    20041 atagtcttca gcatcgagga caagaaggtg caggtatcgt gtccaatgat cgaggaaagc
    20101 ttaaggggca tcgagatttg gggctgcttt ctgaggtatt caaagatcaa agaaaattgc
    20161 agcagttaac aggagaagct gcaatcggac atgtacgtta tgcaacagca ggcaatggaa
    20221 gtgtggacaa tattcagcct tttctattca aatttcatga tcagtcgatc ggcttagcgc
    20281 ataatggtaa cttgatcaat gctcgttccc ttagaaaaga gttagaggaa aatggggcga
    20341 tcttccattc taactccgat acggagatat tgatgcattt gatccgacga agtgaagaaa
    20401 cagatttttt aaataaatta aaaagtgctt tgaatgaagt caaaggtggc tttgcctatt
    20461 tattaatgac agaaacggca atgattgccg ctttggaccc aaatggtttt cgtccattag
    20521 caatcggaca aatgaaaaac ggagcatacg tgattgcctc ggaaacttgt gcattagaag
    20581 ttattggggc acgttttata agagatgtcc agcctggtga gatagtgatc atcaatgacg
    20641 aaggaatcca gattgattgc tttacgaagg acacgcagct ttctatttgt tcgatggaat
    20701 atatctattt tgccagacca gattcaaaca tcgcagggat caatgtgcat actgcacgga
    20761 aaaatatggg acggaattta gcgaaagaat cgccagtaaa agcagatatg gtcgttggcg
    20821 tacctaattc ttctttgtca gccgcaagcg gttatgcgga agctagtgga atcccttatg
    20881 aaatcggatt agtaaaaaat caatacattg cccgaacatt tatccagcca acgcctgagc
    20941 taagagagca aggtgttcga atgaaattat ctgctgtacg aggagtagtg gaaggtaaaa
    21001 aggtgattat ggtcgatgat tcaattgttc ggggtacgac aagcagacgg atcgtccatc
    21061 ttttgaaaga agcaggggca aaagaagtcc atgtgaggat tgcttctcct cctttgaaat
    21121 atccatgttt ttacgggatt gatattcaaa cgcgtaaaga actcattgcg gcaaatcaca
    21181 cgattgatga aatcaaagaa tgtatcggtg cagattcttt aggtttttta agtgaagagg
    21241 gtctgatcga atcgatcggt ctggaaaaag atgctccgta ttcaggattg tgtatggctt
    21301 atttcaacgg agattatccg acgccgttat atgactatga agaaaaatat caggaatcac
    21361 taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa gggagtatat gaattcaatg
    21421 gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg gatatgaagt agtagaacgg
    21481 atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga tgggcgctat aggaggattc
    21541 ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc cagtccttgt ctcggggacc
    21601 gatggcgtgg ggacgaaatt agcagtggca atcgaagcag acaaacacga tacaatcggg
    21661 atcgactgcg tagcgatgtg tgtcaatgat atcttagcac aaggcgcaga gccgttatat
    21721 ttcttagact acttagcaac ggggaaaaat gatccgttac gtttggaaaa tgtagtaaaa
    21781 ggtgtagcag atggatgtgt tcaaggaaat atggcgctga ttggcggaga aacagctgaa
    21841 atgcctggta tgtactcagg taatgattat gatctagccg gatttgctgt agggatagct
    21901 gaaaaatctc aattattgac aggagaaacg atcaacccag gagatgtgtt gcttggaata
    21961 gcatctagcg ggattcattc aaatgggtac tctcttgttc gcaaaatttt ctttgagcag
    22021 cagcagtttt ctttatcatc aaaactgcca gaattgaaaa aaacacttgg cgaagaattg
    22081 cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc tgatcaaaga aaagaaaata
    22141 aaaggagctg cacatatcac tggtggaggg tttgtagaaa atatcccgcg aatgttacca
    22201 gaatctatgg ctgcacaaat caaacttggc tcatggccga ttcttccaat attcggctgt
    22261 ttacaaaaat atggtgaaat tcctgagatg gagatgtatg aaatcttcaa tatgggcatc
    22321 ggtatggtcc tggcagtcga agaatcgaaa gtaaaagaaa tagtacacga aattgagcaa
    22381 gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca aagaaaaaca tcgtgtggtg
    22441 tttataggag aagaacgatg agaatcgctg tttttgcttc tggaaacgga agcaactttc
    22501 aagcgctagc cgattatcta tcgaaaaaag gattggaatc ttcgattgac tggttgtttt
    22561 gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc gttgagtgta ccggcagatt
    22621 gtttttcacc taaagaattt gattcaaaaa aagagtatga agaagcaatt ttgcataaat
    22681 tgaaagaaaa aaagatcgat ttgatcgtat tagctggata tatgcgaatc attggaccgg
    22741 tattgctgaa aaattatgat aaaagaatca tcaatatcca tccgtctctt ttacctgcct
    22801 tccctggttt gcatggcata cgtgatgcct ttgaagctgg agtaaaagaa acgggtgtca
    22861 caattcatta tatcgaccaa ggtgtagata ctggaccaat catccggcag gaaaaagtga
    22921 gaattgaaca ggaggacacg ttcgattctt tagaagaaaa aatccataga gtagagcatc
    22981 gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg aggctatctg aaatgacaag
    23041 agcattgatc agtgtatcag ataaaactgg gattgtagca ttcgcccaag gtttgattga
    23101 acaaggagta gaaatcattt caacaggagg aacaaagcat gcgttggata aagctgggat
    23161 caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa atgatggatg gccgtgtcaa
    23221 aacacttcat cctaaaatcc atggtggtct tttaggaaga cgcgatctcc ctagccatat
    23281 ggaggcaatg gaaacggaaa atatccaacc aatcgacttt gtttgtgtca atttatatcc
    23341 tttcaaagaa acgattatga aaccagatgt cgaaatagca gaagctattg aaaacattga
    23401 tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac catgaatttg tcacctctgt
    23461 tgttgatcca tctgactatg aagaagtact ttcagaatta cgtcagtatg ggcatacttc
    23521 actttctttc agacaaaaac tagcggcaaa agtctttcgt catactgcgg catatgatgc
    23581 actgatcgct caatacttaa cggattgggt ggaagaaaaa gaaccggaaa aattgacact
    23641 tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat agccatcaag ccgctgcctt
    23701 ttatcagtcc gctcttctgg aaaaatattc gatcgcagca gcaaaacaac tccatgggaa
    23761 agaactttct tataataata tccgagatgc agatgctgcg ctacggatca tgaaggaata
    23821 tgacgagcca acggtagtcg ccttgaaaca catgaatcct tgtggtattg ggacagcatc
    23881 tacgatttta gaagcgtggg aagcagcgta tgaagcagat cctgtttcaa tctttggtgg
    23941 gatcatcgtt ttgaatcgag aagtagatct gccaacagca gaacagatgc ataagctgtt
    24001 tctagaaatc atcattgccc cttcttatac acaagaagct ttggaattac tgcaaaaaaa
    24061 gaaaaacatc cgattactga cagtagactt tgcgcaggaa gaaaatcaaa atcgagaaga
    24121 aacggtctca gtcttaggtg gacttcttgt acaagatcaa gatctggcaa tagaaaatga
    24181 agaagagtgg aaagtcgtga caaaacgtaa gccgactcca gcagaagcaa aagcattagt
    24241 gtttgcttgg agagctgtca aacatgtgaa atctaatgcg atcgtactgg caaacgaacg
    24301 tcagacagta ggaatcggag ccgggcagat gaatcgagtc ggttcagtaa aaattgcgat
    24361 tgaacaagcg caagagaaaa tggaaggtgc agttttagct agcgatgctt attttccaat
    24421 ggatgattcg gttgaatatg cagccaaaca tggtataaaa gcaatcattc agccaggagg
    24481 aagcatcaaa gaccaagatt ctattgatat ggcgaataaa tacgatctag caatggtctt
    24541 tactgatgtt cggcatttta gacattagag aggagtcgga taaatgaaca ttttggtgat
    24601 cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt ttagccagtc cgaaggtgga
    24661 tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa gatggtatca attgtgtcgc
    24721 tatcagtgag cgagatcatt cagcgttaat ccattttgtt aagaaagagg ctattgattg
    24781 gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc gtcgatgatt ttatggcgga
    24841 aggattgaaa atcttcggtc cgacaaaagc agcagctctg atcgaaggtt cgaaaagttt
    24901 tgctaaacaa ttaatggata cttatcaaat cccgacagct aaatccagaa gttttgaatg
    24961 ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg ccaatcgtag tgaaagcgga
    25021 cggcttagca gctgggaaag gtgtagtcgt ggctaccaca gtgaaggaag cgctggatgc
    25081 ggcagaacaa atgttgaacc agcatcgatt tggggatagt agcaaaaaaa tcgtcgtaga
    25141 agagtttttg gcaggagaag aattctccct tttggcgttt gtccgtaata ccaaggtgta
    25201 tccgatggtc atttcgcaag atcataagcg ggcatttgat aacgataaag ggccaaatac
    25261 aggcggaatg ggtgcttatt caccagtacc gcaaatttca aaaaagatga ttgaaaaagc
    25321 tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa gaactaggac gctctttcac
    25381 gggtgtttta tatgcgggtt tgattgctac gaaggaagga ccaaaagtga tcgaattcaa
    25441 tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct cggttgaaga gtgactttgc
    25501 gcaagtaatc gatgatttat tagagaatcg tacagtagag ttaaagtggc agcaagaagg
    25561 atacaatgta ggcgttgtgg ttgcagctgc cggttacccc gaagaatatg agacaggaat
    25621 ggttctacca gattttctgg aagaagagct ttctgtttac tatgctggag tttcctcaaa
    25681 taacggcaaa ctagttggag caggaggacg tctttattta gtagaagcat acggtgagac
    25741 gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg gataaaacga cagatggaac
    25801 attttatcgt acagatattg gagaaaaagc tgccaatagc gaactgatcg tgaagtaaat
    25861 cagctgcttc catttataaa aaatacaaca aacataatcg gaagcgccga ttttagtgaa
    25921 gccttttcat aaagagtgtg gtacactttt gttgtgtata taaataagga ggagttcaga
    25981 tgaaaaaaat tcaattaggt acttctgatc agaaagcagc tgcagtgatc ttaggctgta
    26041 tgcgtatcaa caatgcggaa gatccagtaa aagtgattga gacagcttat gaaaacggca
    26101 ttgatttttt tgaccatgca gatatttatg gaaacggtga atgtgaagaa atctttgcag
    26161 atgctctagc aaaaacatca atcaaacgag aagatctttt tattcaaaca aaatgtggaa
    26221 tcgttccagg tgtgatgttt gatttttcta aaaaacatat cattgaatct gtagaaaaaa
    26281 gtttgacccg tctgaaaatg gattatgtgg atgctttact tcttcatcgt ccagatacat
    26341 tggtagaacc agaggaagta gctgaagcgt ttgatgaatt ggaacggtca ggaaaagttc
    26401 gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga attgctgaaa aaatatgttc
    26461 gtcaaccatt agtagctaat cagctgcaat ttggcttgaa acatactgga atgatcgatc
    26521 aagggattca tgtcaatatg acagatgaag caagtatcga tcatgacggt agtgtacttg
    26581 attattcccg attgcatgat atgacgatcc aagcatggtc accataccag tatggatttt
    26641 ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt aaaccaaaca ttagaagaac
    26701 tagcagctaa atatcaaacg acaccaactg gcttggcttc tgcttggatt ttacgccatc
    26761 cagccaatat gcaagtgatc gccggtacga tgaacgtagg gcgaatcaag gaaatcgctc
    26821 aagcttcaga aattaatatg tctcgggaag attggtacga attgtaccgt gctgctggaa
    26881 acattttacc ttaaagattg tcataagcag gtcaaaaggg ctataaatta aaaagacaga
    26941 ataacttgaa agctgttttc agcttcgaga tattctgtct ttttttattg caaatggaaa
    27001 gagtatcatt gtacggattt cgtttattag ttatttttgt ttttaaggaa aagctgtgtc
    27061 cgatattctt ttggagaaat cccttgtttt ttcttaaata aataactgaa atagttaggg
    27121 tcattataac caatttcaaa agcaatatct gaaatacgat agtctgattg ctgcaataat
    27181 ttttttgcta attcgattct ttgttctgtc agataatcga tgaatgtttg gttaaacgat
    27241 tgagaaaaaa tcgtactgaa ataagaggga cttaatgcaa cttgttgagc gacccaagtt
    27301 aaggacatat ctggatcggt aaaattttct ttgataaaat ccaatgcatg gttcaaaatt
    27361 ggctgatatt tgttccttga aggctgtgag ggcagagcag ttaagaaaga aatgaacata
    27421 taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg ctaagcgttg actggtggat
    27481 gtgcaatgaa ccatttcttg cgggattttc ttttctttca ggcagacatg ctggagtagc
    27541 ttagtcaaga caaataaacg atataatcgt gttcgagttg gtgtgccttg tggtttcgtc
    27601 aagtgttcga tgtacttaga tacttcgttt ttcgataatg aagacaaatc aagtgagagg
    27661 tcaaagtaaa agacagaatc aactggaagt ttcgcttctt gaatcaaatc ctcatagctg
    27721 ataatttgtt ctttaaggga actggcggtt tcaagtatca tctgcgtgat ctcaaaagat
    27781 gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg cgacagaaaa attatttttt
    27841 tgtccctttt ctaattcatg gaccaacgtt tgggctacct gataactggt ctcgatcaca
    27901 ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc gagaggaaac agcagagaag
    27961 atcacttgtt gattctccct aaataaaaac ttcaaataat cactgagatg agcgtaatct
    28021 tcaaagtctg tactgacttg gtttgctgct aataatacgg tcattttttt ccctgctaaa
    28081 gagcgattga aagattcagc aagtttcaat gtttcactga tcgtaagtga gcctttgaac
    28141 aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa gatcagaaga agtgtgcgtt
    28201 tctttttgct gcaaatctaa agcagttact gctttttcta ttgcttgttt caattcgcta
    28261 cttttaatcg gtttaagcag ataatcatct gcctgtacct ggattgctcc tttcatataa
    28321 tcgaaatcgt cgaatccact aataaatatg agacgagtcc aaggaagtag tttccttgct
    28381 tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga tatcagtcag aatgatgtcg
    28441 ggctgtaagt cgataattga agacagtgcc atctcaccat ctccagcttc gccgacataa
    28501 gtgattggat aataagaacc aaggacagtt atttgttccc gcaagttatt gcgaatgagg
    28561 tattcgtctt cgatgataaa aactttatac atgtttgttt cctccaattg ttttgggaga
    28621 tagagggatc atcagtgtga cagttgttcc ttctcggtat ttgctgtcaa tcgtgatagg
    28681 tttttgatca tttccgtaat aaagaaggag acgcttgttt acgttgtaaa gtccgtagcc
    28741 gatactgaaa tctgaatcga tccctttggc taactcttta cgtatttctg ccagtttttc
    28801 tgcagggatg ccaatcccgt tgtctataac tttgaattgg atcatctcat caattttgca
    28861 tgtggtaata cgaataaatc ctttgtgacg gacgtacttc gttccatggt atacagcatt
    28921 ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca ggaaaaagtg tttctggtat
    28981 atcgatctta tagtcaagca tatggccgta tcttattttt aagatgacta gataatcatt
    29041 gatatggcta atttcttttt caagaggaat ccagtcttgt cctttgctta aagacgtacg
    29101 aaaaaagccg gaaagtgcat gggtcatagt cagagcttct tcaaagcagt cttgttcgat
    29161 caaagaaata atggcatcca aactgttata aataaaatgg ggagtaatct gagcttgcaa
    29221 tacacgaact tcactttgtg ctaaatggta ttgtttcaaa gcgttctccc gtaaagactg
    29281 gtttagatct tccgccattt tattcatgct agcggtaagt gtttgtaatt ccagtgtttc
    29341 cggcagggtt aaacgaaagc caaggttccc tgaagaaagt tgttctgaca tctcgatcag
    29401 ctgattgatc ggttcttgga cctttctctc taaaaagcga cgattctttt tcgcatagaa
    29461 aaaaatggta ctaagaactc caagttccac tattgttaat agaatgagcg agcggacaag
    29521 tgtcgtattt tttttgcttg ctaagtcgat ctccacacga acaaaatctt gtaagatatc
    29581 tgacagcagt tgagtgacag aatctacctg attgatgatc tcttcattgg tatccataaa
    29641 atcttttgcc aagatattat tgatcagctc gttttgataa tcttccagtg tatcgatcgt
    29701 acgtagcgat acatctaacg tactaatttc tttaagtgta tttgtattat ttttgatctc
    29761 tcgaatatca ttcctaagtt cttctaggat attttcctca ttgaactcat ctacggttac
    29821 ttggccagaa atcaagtccc acatactttc caaaactttt tgatcaacct tcaaagcaat
    29881 tgcatctgct ttttggatat ttgcgatcgt ctgttgataa atcaaaagct gttgcgaata
    29941 taacagtatg ctcagtagca aagggataac agcaaataga agcatggctt tgtttgcttg
    30001 ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata cttctccagt caataatttc
    30061 tggttgggat gttagctata ttctgctggg aaaagacagt ctcggaaata aaaatatctt
    30121 taggtaatgt ttttcctgtt tgaatatttc caaaataacg agtaacggta ttggctacat
    30181 accagccgac atcaggttta cactcgacta cacaattgac gattcctgag cgtagcttct
    30241 cgatcatatc ttcttgtcca tcaatagtta caatgatcaa atcttctcca ggtttgattg
    30301 atgtttcttt tatcgcagct aaagcaccgt gagtcatttc atcactatgg ctgtataata
    30361 catcgatttc ggataaatca tttgtttcta aatatcgttt catctggtct tttcctttca
    30421 tccgaataaa atcaccgaca attcggtgaa caatagtcat cggaggttta tggtcgatgg
    30481 ttccatcttt ttttagggtt ggtttacgat tgattgattc caaaaatcct tcggagcgta
    30541 gcttggtcgg tgtggagttc tctgaaccag caagttctag gattctgatt tcctgtttct
    30601 ttgaatttgt gaagtgattg ctgacaaata aacccgcccg atttccttct gctttgaaac
    30661 tggggccaat gtgggtgacg aaaagctcgg gatcattcgt ttgaatatgt ctgtcgacaa
    30721 gtatgacagg gatccctgct cgctttgctt cttttaatac agagtcccac ccggattctt
    30781 gcaaaggagt aaaaatgatc atatccactt tataggcaat gaacgtccgt atatcttgga
    30841 tttgccgttc ctgattcatg tagccatttc ggtagagaac ttggtaatct tctttttcaa
    30901 gcgcagtgat aattgattca gtatgctttt tccgccagct actctccgtt cctgattgcg
    30961 aaaaaccaac gactagtggg tcaggtgctt cctttgtatt aggtgtacac cccatcattt
    31021 ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg tttcacttta aatcactcca
    31081 tctggatttg ttagtactat cttatcagat aaaagggaaa atcaaagtca aaaggaatca
    31141 atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa aaaaagtaca cctaaaaaat
    31201 ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc tttattagct atactcggtg
    31261 tatcaattga aatgggggat aaaaaatgaa aaacaaattt agaaagattg ttgggactat
    31321 cgctattttg accgcaagca ttctattaag tgcgtgtggc aattcaggat ctgcagatga
    31381 tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat ggatcgctga
    31441 tgggaacaac atggtctcgg aattagaaaa actgggttat aaaacagatc ttcaatatgg
    31501 agaagataaa gtagaaaatc aagtagcaca aatagaaaat atgattacaa aaggggtaga
    31561 tacacttgtg attgcttcta tcgatggttc ggctttaact gatgtgttag ctaaagcaaa
    31621 agaagcagat atcaaggtga tcgcctatga tcgtttactg atgaattcag aaaatgtcga
    31681 ttattacgcg acttttgata attttggagt aggtgtatcg caagcagctt atattgaaga
    31741 gcacttagga ttgaaagaag gaaaaggacc attcacgatt gaattatttg gtggctcgcc
    31801 tgacgacaac aacgcactaa tcaattacaa cggagtgatg tctgttttac aaccatatat
    31861 ggacaacggt cagttggtcg tgccatctgg acagacgagt ttcagtcaaa tcgctacgct
    31921 tcgctgggat ggttctacgg cgcaagcaag gatggataat ctgttaagcg caaattatac
    31981 tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca ataagtttag gaatcatttc
    32041 gtcattaaaa ggcgtggggt atgggagtga aagtaaacct ttacctgtga ttactggaca
    32101 agatgcgaca gttgctggcg ttaaatcgat cattgccggg gaacaaacac aaacgatctt
    32161 taaagacaca cgtatcttag caaaaaatac gattgaaatg atcaaagcaa tcagtgatgg
    32221 agaagaggtt ccggtcaatg atactgaaac ttatgataat ggggtgaaaa ctgtccctac
    32281 gtatttagca aatacagtat ctgtcgataa agataattat caagcagagc tgatcgacac
    32341 cgattattat aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg aactaaagag
    32401 atctaagtca gagagaggat aagaattatg gcggattgta ttttaaaaat ggcagatatc
    32461 accaaaaaat tttctggaat ccgagcgtta gattgcgtga atctgatgat tgaacgtggg
    32521 gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat caacattgat gaatgtcttg
    32581 agcggcttgt atccatatgg ctcctatgac ggggaaatct tttatgatgg aaagctgtgt
    32641 caatttaaag atttgaaaga tagtgaagaa aaaggcatcg tcatcattca tcaagaatta
    32701 gcactaagtc cttatctttc aataaaagag aatatttttc tcggaaatga acaagcagaa
    32761 agaggcgtga ttgattggga actgacagaa aagaaaacat atgaactgct gaataaagtc
    32821 ggtttgtcta tcgacccgaa tcttctagta tcccagattg gcgtagggca acagcaacta
    32881 gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga tgagcctacc
    32941 gctgcattga atgaagaaga aagcgcgaat ttgctgagat tgatccgaga gttgaaagag
    33001 caaggtgtaa cctccatcat tatttcacat aagctgaatg aaatcgtgga tatcgcagat
    33061 cgaatcacca ttttacggga tggacagacg attgagacac tagaaaatga aatgatcagc
    33121 gaagagcata ttattcgcgg aatggtgggc agagatttag ttgatcgtta tcctgaaaga
    33181 aaaccggata ttggagaagt ttattttaaa gtagaagact ggacggtcta tcaccctctt
    33241 gatcacgagc gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg agagattgta
    33301 ggaatagctg gactgatggg agcgggcaga actgaattcg ctatgagtct atttggacat
    33361 tcttatggaa gcaagatatc cggacgtata ttaaaggaag gggcagagat atcagttaag
    33421 accgttcctt cagccattgc aaacggactc gcttatgtct cggaggaccg aaaatcttta
    33481 ggactgaatc tgctgatgga tattcgggaa aacgtgacgc tgagcagttt ggataaaata
    33541 agtcaaaaag gagtattaga taaagaaaaa gaagtaactg aggcagaaaa tttccggaaa
    33601 aagatgcgta taaaaacaaa ctccatccat caaattgtca gtagtcttag tggtgggaat
    33661 caacaaaaag tcgttttatc caagtggctg atgacacaac ccgatattct ttttttggat
    33721 gagccaaccc gtgggataga tgttggggca aaatatgaga tctacacgat tatcgaagaa
    33781 atggcagcaa atggcaaagc tgtctgtctt atctcttctg aacttcctga aatattaggg
    33841 atgtgtgatc gaatctatac gatgaacgaa ggacgtatta caggagaagt ctcgcgagaa
    33901 gatgcagatc aggaagtctt gatgaaatta atgacgaaag aagaggtggg ttaagttgga
    33961 acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa gtagagcagc
    34021 tgatcttttt ggtcgctaca gcatggtgat cattttagcg ggactactta ttgcgtttca
    34081 attgatgacg gatggtattt tctggcgtcc attgaatata actaaccttg ttctgcaaaa
    34141 tagccatata ttagtactag ctgcagggat gttattagtt gtgctcttag gtcatgtgga
    34201 tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga tgatggtcaa
    34261 tcgccaaatg aacccgtgga tgacagttat cgcatgtctt gcagtaggcg ggctgatcgg
    34321 cgcatggcaa ggtttttggg tagcttatgc agggataccg gcgtttatcg tgactttagc
    34381 aggattgctg atgtttcgtg gactgacaca agttgttcta ggaggccaat ctctagcacc
    34441 ttttccagct gttttccaaa aaatgtctac gggttattta ccagacttaa gcgaaggaag
    34501 cagccttcac ttcttgacat tgattttagg aatgatcatc acgatcgtgt tagttgttgg
    34561 tcaatggcga acaagaaatc gtcggaaaaa tcatttattt gaagtggaat ctccaacagc
    34621 tttcttggta aaggcggtat ttacagcagt cgttgtacta ggaataactt atatctttgc
    34681 ctcttatcaa gggtatccga ttattttgat cattcttggt ttgatcgttg cagcatatgg
    34741 gtttttgaca aataaaacag tagcaggtcg acagatttat gcgacgggcg gaaatcgaaa
    34801 agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat tcgtcaacat
    34861 gggagtcatg gcagctttag ctggattagt tttagctgct cgattgaatg cggcaacacc
    34921 tcaagcaggt acatcgatgg aactggatgc aatggcggca gtatattttg gtggtgcttc
    34981 gacttccggc ggcattggaa caatcatggg tgcgattgtc ggtggcttgg tcatgggtgt
    35041 attgaacaat ggcatgtcga ttttaggcgt cggggtagac tggcagcagg caatcaaagg
    35101 attgatcttg cttttagccg ttgtgctaga cttgtataat aagaaaaaac gaacaacgta
    35161 ggagaacgcc ggttattcag aaaaaagact gtgacaagac atttgtcaca gtcttttttt
    35221 cgctaaaagc gaacattcat tgtttcgttt gtatagacta aaaataatat atgttttttt
    35281 accattcgta taaattctct ttttgctctt gatttttttg cttaattgag ttaaattaca
    35341 tagggcgcta aaaaaagcct tgattaaatt aataatgttc gtgttttgct ggatgtgaac
    35401 atcaggagga taatgtaatg aaagtatttg attacgaaga tattcaatta attcctaata
    35461 aatgtatcgt aaatagccgt tctgaatgtg atacaacggt cacattaggt aaacatacat
    35521 tcaaaatgcc agtagtgcct gccaacatgc agacgattat tgatgaaaca atcgcagaat
    35581 tcttagcgga aaatggttat ttctatatca tgcatcggtt tgatgaagca gctcgaattc
    35641 cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt ggcgtcaaga
    35701 aagaggaata ttcctttata gagaagttgg cagaagaatc gctaaatcct gattatatca
    35761 ctatcgacat cgcgcatgga catgctaact cagttattga tatgattcag catattaaaa
    35821 aatatttgcc tgaaactttt gtgattgccg gtaatgttgg tacaccggaa gccgtccgtg
    35881 aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat tggtccagga aaagtatgta
    35941 ttacaaaaat caagacaggc tttgggactg gaggctggca actggcagct cttagatggt
    36001 gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca catggcgata
    36061 ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg tttgccggtc
    36121 acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa gaatactttg
    36181 gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag aaaatctgga
    36241 tccaacacaa aggatctttg aaagatacac tagtggaaat gcaacaagac cttcaatcat
    36301 ctatttctta tgctggagga agagatctgg aagccattcg aaaagttgac tatgtcatcg
    36361 tgaaaaattc gatttttaat ggagacacaa tctaaaaaat aaataaaata ttaggatgtt
    36421 gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa acaaaaatag ttctttatat
    36481 gttttgtttg gctggaatta ttatcgcaga tacaagcttt tgaacatcat tattcagaat
    36541 aagggactgt gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt
    36601 ttttctcgta aattcggtaa aatcaaaaga aaaaatagac aatagaaaag agaagcttat
    36661 gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt caaattatgg aacagattat
    36721 gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact
    36781 agggcaactt tttggtgtga atcgttccac cgttgtccat gcattggaag agcttaggga
    36841 gcttggggta ctaacgagca aacgtggcag cggatggttt gttaatcaga ctgaatgggg
    36901 gaaattcgct gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt atgagcagac
    36961 ggactggtat gagcaaaaaa taagagaagc aaagcagatc caaaaaacat cgtttcttga
    37021 tttatacgct agcgagatgc cagatgagct tttgccgaat tttgagtttc cttcttattc
    37081 tcttgaagaa atcttagcgg aagaaaaaga aatggatctt ttaggctata ctccgttacg
    37141 taacaaaatc aaacggtatt tggaaagtaa atttgaattg gcattttccc ccaaccagct
    37201 tcttgtgact ggtggcggtc agcaagcaat ctttttgatt ttacaaacga ttctttcagc
    37261 tggagagggg atagctgtag agtcaccttc ttttttttac cgattacctc ttttcaaagc
    37321 ttcaggaacg cgtttatttg ggataccgat ggatgaagaa gggatcgact tagaaaaact
    37381 gaaagaatcg atccataaaa ataaattgaa agcagtgctt atcaacccga attttcaaaa
    37441 ccctacagga aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca
    37501 gtatcggctt ccaattattg aagacgatgt atttacggat ctttcatttc taaaccaaga
    37561 agaagtcctt cctatacgaa cgattgatcc ggataatgtg ttatacgtcg gttcgttatc
    37621 tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga cttttatctc
    37681 gcaactagct caagcgcaag aaatgatgga gttttcgatg aatatcctta cacaaatcac
    37741 agtggctaat gtatttaatg aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt
    37801 gaaagaaaaa agccgactga taagcgcttg gggagattca caatcgtttt tcaagcttca
    37861 ttcaatttta ggtggttact atgcctggat tacttgggat gggaaaaaac tagaccccga
    37921 actagcagaa aaagtagtag atatgggtct tggaattgca ccaagtctac tttttgggca
    37981 agatataaat ggaatcagaa tcaattacag taggctggat gaaaaccaaa taccgttgtt
    38041 ttattcaaag atgaaaattc tagaaaagtg gttaacagaa taatttcaca aagtaaatag
    38101 attaaagaat ataacagctt ggatttaaat ttcaagctgt ttcttttttt tcggaaaaag
    38161 aatacgatat aaagaaacta agaaaaaacc attatttgta cgtccgttca cgatgtgtaa
    38221 ataaaataaa tgtttgcaac aaaactgtat aacaccaacg tttttaaata gaaacagatg
    38281 gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa atataaaacg ctttccttat
    38341 tgtgatttta ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga
    38401 aaatttgtgc ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg
    38461 ttaaatagtg atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata
    38521 gatgagagag gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac
    38581 tgctagatga agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg
    38641 tagttaacaa agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc
    38701 atatggtatg gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc
    38761 taggcttttt tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat
    38821 ttgataaaga agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg
    38881 gtttaccttt atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc
    38941 cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag
    39001 ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg
    39061 gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc
    39121 atgcaaatgc cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa
    39181 aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc
    39241 aagtcgatgg aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg
    39301 cgatcagtgg aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata
    39361 cactatctgg tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga
    39421 aaaaagatcc attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag
    39481 aaaaagaaga acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag
    39541 ccgacaaagt accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc
    39601 cacaatcaat caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc
    39661 acaaaaaaca atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga
    39721 gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga
    39781 aggattgcag gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg
    39841 aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca
    39901 attctttggt tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag
    39961 atatcgaatg agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg
    40021 tgttcataca ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg
    40081 tattcgagta gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat
    40141 tagaagtaac gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga
    40201 agaagtgccg gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg
    40261 aacagatgta tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga
    40321 caatcttgca aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt
    40381 agatgtagaa acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga
    40441 agcacaacga caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat
    40501 cctttcatta gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg
    40561 acaagcagaa aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat
    40621 cgcggaattt taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaatt
//
LOCUS       DACEJZ010000013        38956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEJZ010000013 DACEJZ010000000
VERSION     DACEJZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.013"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2198)
                     /locus_tag="IW656_000662"
     CDS             complement(<1..2198)
                     /locus_tag="IW656_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836390.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
     gene            complement(2185..2403)
                     /locus_tag="IW656_000663"
     CDS             complement(2185..2403)
                     /locus_tag="IW656_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836391.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2405..3448)
                     /locus_tag="IW656_000664"
     CDS             complement(2405..3448)
                     /locus_tag="IW656_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0836392.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3603..3929)
                     /locus_tag="IW656_000665"
     CDS             complement(3603..3929)
                     /locus_tag="IW656_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836393.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3937..4140)
                     /locus_tag="IW656_000666"
     CDS             complement(3937..4140)
                     /locus_tag="IW656_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0836394.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4215..4865
                     /locus_tag="IW656_000667"
     CDS             4215..4865
                     /locus_tag="IW656_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0836395.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4920..6086
                     /locus_tag="IW656_000668"
     CDS             4920..6086
                     /locus_tag="IW656_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0836396.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IW656_000669"
     CDS             complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IW656_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0836397.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IW656_000670"
     CDS             complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IW656_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0836398.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7545..8012)
                     /locus_tag="IW656_000671"
     CDS             complement(7545..8012)
                     /locus_tag="IW656_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0836399.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8132..9670)
                     /locus_tag="IW656_000672"
     CDS             complement(8132..9670)
                     /locus_tag="IW656_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0836400.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9750..10436
                     /locus_tag="IW656_000673"
     CDS             9750..10436
                     /locus_tag="IW656_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0836401.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IW656_000674"
     CDS             complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IW656_000674"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0836402.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IW656_000675"
     CDS             complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IW656_000675"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0836403.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11922..13052)
                     /locus_tag="IW656_000676"
     CDS             complement(11922..13052)
                     /locus_tag="IW656_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0836404.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13052..15235)
                     /locus_tag="IW656_000677"
     CDS             complement(13052..15235)
                     /locus_tag="IW656_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0836405.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIAPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15232..16257)
                     /locus_tag="IW656_000678"
     CDS             complement(15232..16257)
                     /locus_tag="IW656_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0836406.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16302..17129)
                     /locus_tag="IW656_000679"
     CDS             complement(16302..17129)
                     /locus_tag="IW656_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0836407.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17126..18193)
                     /locus_tag="IW656_000680"
     CDS             complement(17126..18193)
                     /locus_tag="IW656_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0836408.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18219..19055)
                     /locus_tag="IW656_000681"
     CDS             complement(18219..19055)
                     /locus_tag="IW656_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0836409.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19052..19921)
                     /locus_tag="IW656_000682"
     CDS             complement(19052..19921)
                     /locus_tag="IW656_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836410.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19991..21274)
                     /locus_tag="IW656_000683"
     CDS             complement(19991..21274)
                     /locus_tag="IW656_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836411.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21288..22973)
                     /locus_tag="IW656_000684"
     CDS             complement(21288..22973)
                     /locus_tag="IW656_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0836412.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23248..24273
                     /locus_tag="IW656_000685"
     CDS             23248..24273
                     /locus_tag="IW656_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0836413.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IW656_000686"
     CDS             complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IW656_000686"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0836414.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IW656_000687"
     CDS             complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IW656_000687"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0836415.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32945..33913
                     /locus_tag="IW656_000688"
     CDS             32945..33913
                     /locus_tag="IW656_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836416.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33931..34836
                     /locus_tag="IW656_000689"
     CDS             33931..34836
                     /locus_tag="IW656_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836417.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34833..35639
                     /locus_tag="IW656_000690"
     CDS             34833..35639
                     /locus_tag="IW656_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0836418.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35796..36764
                     /locus_tag="IW656_000691"
     CDS             35796..36764
                     /locus_tag="IW656_000691"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0836419.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(37081..37605)
                     /locus_tag="IW656_000692"
     CDS             complement(37081..37605)
                     /locus_tag="IW656_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0836420.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            37892..>38956
                     /gene="scm"
                     /locus_tag="IW656_000693"
     CDS             37892..>38956
                     /gene="scm"
                     /locus_tag="IW656_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0836421.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        11159 a         8195 c         7048 g        12554 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttc ctcacgattc attgcttcag gcgtgtattt tgcttcctgt
      301 cgcttctgat taattaatac ttcagcagac ttagcttgac cgtgcatagc atttttacgt
      361 tcttcaatat cttgtttaac tttttgctga attgcacttg caggattaat atttgattca
      421 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcagcgttt
      481 tcaactttag cgccatcttt tctatccgaa agacgtgttg gcatagaatc agcatatttt
      541 ccagtttcat cttctttgtt tttagctact ataggtttgc tatttttatt aatagcgtac
      601 acggtaccat catctaaacc ttctttgaat ttattcttgg tatcattagc catatcacgc
      661 atactcttaa atggttttgt tgctctttca ctggccattg tagctccatc cgaagctaag
      721 cctgataatc ctcgttcacg agcataccca agtgttttcc ccgttttatt agctttttga
      781 gaaagtgatt gaccaaagtt gcgttcaggt tttcgttctt ccccttccgc ttgatcgttt
      841 ccaccactat ttttcccacc tttagatcgg ttcataccac ctgttccttt acgaaacagc
      901 atagctccca tgccaaatgc tgaagcaagc tggccatagc cttcttttaa accaatatcc
      961 acgccaaagt aacgcatgac tgttccacta ccctttatca gcacaataac agcgcagacg
     1021 tacgctactg cttttataaa aggatttaca gaagaaccgc tacctgttcc gagataatta
     1081 acaaacattg caaacataga caatccgaac ccttgaaatc cgactgtcaa ataacactgt
     1141 aaaatatctg acaaaacttg ctttgatcgt tggcctgttt ccaagtcagt agcaaatact
     1201 aaaactccaa gtacgcgttt aaacgctagc tcgagtattg ctgtaattat aatgaaagca
     1261 ctaaaaacat acgctaccgc taaagcaatc aatccaaaag aaatccccca tagatttgct
     1321 tgatagcgat aatatcctga tttaatgccc tcaacaaagg tcgacagaaa attatccgaa
     1381 aatttggtag caacaaattc attatcagaa ttttcaacta attcataacg cagattatca
     1441 gctattgggt tctctttttc cattttgtcg agtacattat ctgtcaatac ttgcgtaaga
     1501 tccgttttcc aaaaattttc ttttgttaga ttgttccggt cattcgtttt atcgatcatg
     1561 gaatattgat ctgttttatt gatataagct aaatcagtaa ccccttgttc aatcagcgac
     1621 cacgccacgc cttcgtccga atttgtaatc gtagtcgtat cattataaaa tgttttagaa
     1681 aactctattc cgctgtgaat caacgttggc attaaaagaa tcaatgaaat tgacattaca
     1741 acattcattc caacagattt tagatcaacc gttcccttac ctgtaatcat tttataacca
     1801 ataatcacta gtgaaagtat cattaagcca acaacaatgg tacttaaaac agcttgatag
     1861 actttgttca atccggtact ttctacaaag ctaaacaaat ctaaaacttc gggaatcata
     1921 ttttgaatgc catcgacaac agcgaacatt gattttataa cagaccagcc tatccacata
     1981 ccgaactggc tgtaaaaatt tccgttgtat tttttcaaat aattctggta aagcggatta
     2041 gttaaaaact cagtttgact ctcagtatca tcaaaccaat tgaaaatact gccttctgca
     2101 atgttactcg taaaaagaat cacagctatt agaacaagaa aaataaccgg taaccaattt
     2161 gtttttctat tagcaatctt ttttttaatt attttcattg ttacctccct cagcccattt
     2221 tcttaggctc tcagagagtt tctgagctat ttttctgtca attatgatat gagttacgta
     2281 atcatcatta acagctttta aatcgagaac aatagtattt gagcagatac tctcggttaa
     2341 ctcaagagta tctgcgtttt gatcaagaca gtgaatctta tctactagat gattttcaat
     2401 cattttaaat tccctctttt ttttgtagct tgattagttc taacgctttg ttttttagag
     2461 aagtaaagat ataattattt ttttgtttga tacgatgatc tgtttttgct cttcgtatca
     2521 agtcacgtaa agtcattgct agctgttctc taacatgatc tacaaaataa ctattattac
     2581 aaatttcata gataattttc agttgaactt tttgttcttt ttcagcagat ttaattgcat
     2641 aattgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta gccagtttta
     2701 agcaattcac aatactttct ccccacattt gtttaagaag attttcttca ataaatgttt
     2761 cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaaa agatcatcat
     2821 tatgtgattc tttaatatct ttactatctt tataagactt atataattct agttcatttt
     2881 ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg catagttcaa
     2941 attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga actatcaagt
     3001 ttttttgact attttcttga tgggttatct tattaatttt ataaacatca tctttgcaca
     3061 gtacaggctt taacaaataa taaaaatttg ggaacatttg cattttattt ttattgaagc
     3121 cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct gataagattg
     3181 tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc aaaaaaattt
     3241 tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct tttagtaaca
     3301 tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg tttaaaaaaa
     3361 taggatattg atagtataat tctgcatata tatcagaatt agaatagcct tgcttttcaa
     3421 ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt atttgctata
     3481 atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg atctgcaaac
     3541 tgacgggaac actcttttta attttttcta cttcatcatt attcaaaaaa acacctcgtt
     3601 ttttaaattt gatgactcaa tatccatttt ttgcattctt cttttgaaaa caactccatt
     3661 ttttctaata aaataactgg caatcccatt cttttaaatt tcaaaaaagt tgtatatgat
     3721 acattcaaat atttagccat ctcttttcct tttagaaatg gtctttctaa cccggtatca
     3781 attcttgctt gatcaatagc ctcttttgtc atttcaaaaa cataattttt caattgattc
     3841 gtttgttgtt tatctaacat tactgtaatt cctgcatcca ttatttttct cctctcattt
     3901 tctatttctt caatcagcca actcgccaag ttgtatttat ttagatttca aatcattaag
     3961 taaccaattt accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc
     4021 gattttgaca tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag
     4081 tataagatcg gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat
     4141 tcagcgcctc cttttaaatt attcactttg tggtttttat cttaataagc acattttaaa
     4201 ccacgaaacg agtaatgtca attaaaacat tactcaattt gtggttttta tgctataata
     4261 gtttggaatt tactataggg ggtgttactt tgaatagaat aaaattttat attgattcca
     4321 gtaaaaagac cattaaacaa attagtacag aaactggtat taactataca actcttacga
     4381 actattaccg tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt
     4441 atttccatgt tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac
     4501 ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata
     4561 ttgccagtct tgactcagat tattttactg aattatctaa tctatacttt tccatgactt
     4621 ctttacttga agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc
     4681 tagaggactc ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag
     4741 ctttaaactt aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa
     4801 cccaagtaca ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc
     4861 aataaaacta attattcaat cagccaactc gccaagttat cagattggag agattagtta
     4921 tgtcaataca aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc
     4981 gtaaagatga aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag
     5041 aagccaagtt acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc
     5101 cttctaatga cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg
     5161 tacaagaaac gacatatcaa cgtacaaaag accttttccg cctgcatata ctaccaaaat
     5221 tcgggaataa gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat
     5281 gggctttaaa attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt
     5341 atgccatttt tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc
     5401 gggaaaagaa agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata
     5461 ttctcaataa agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg
     5521 gtatacgtaa aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc
     5581 taatgtatgt tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa
     5641 aaactgtcag gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg
     5701 atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc
     5761 agtttggcat aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg
     5821 gtgacattaa tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga
     5881 agaaatataa atttaagcac gttactattc atggattccg gcacactcac gctactcttc
     5941 tactagaagg tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg
     6001 aaactaccct taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa
     6061 aattcattgc ttacactggt ttataatttt gtcattacct ttctcattac cagaacgaaa
     6121 aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg
     6181 ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     6241 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     6301 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     6361 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     6421 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     6481 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     6541 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     6601 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     6661 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     6721 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     6781 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     6841 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     6901 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     6961 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     7021 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     7081 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     7141 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     7201 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     7261 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7321 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     7381 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7441 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     7501 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     7561 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     7621 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     7681 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     7741 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     7801 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     7861 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     7921 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     7981 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     8041 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     8101 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     8161 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     8221 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     8281 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     8341 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     8401 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     8461 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     8521 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     8581 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     8641 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     8701 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     8761 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     8821 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     8881 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     8941 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     9001 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     9061 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     9121 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     9181 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     9241 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     9301 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     9361 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     9421 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     9481 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     9541 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     9601 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     9661 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     9721 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     9781 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     9841 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     9901 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     9961 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
    10021 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
    10081 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
    10141 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
    10201 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
    10261 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
    10321 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
    10381 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
    10441 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
    10501 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
    10561 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
    10621 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
    10681 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
    10741 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
    10801 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
    10861 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
    10921 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
    10981 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
    11041 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
    11101 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
    11161 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
    11221 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
    11281 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
    11341 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
    11401 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
    11461 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
    11521 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
    11581 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
    11641 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
    11701 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
    11761 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
    11821 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
    11881 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
    11941 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
    12001 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
    12061 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
    12121 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
    12181 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
    12241 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
    12301 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
    12361 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
    12421 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
    12481 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
    12541 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
    12601 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
    12661 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
    12721 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
    12781 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
    12841 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
    12901 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
    12961 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
    13021 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
    13081 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
    13141 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
    13201 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
    13261 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
    13321 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
    13381 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
    13441 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
    13501 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
    13561 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
    13621 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
    13681 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
    13741 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
    13801 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggag
    13861 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
    13921 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
    13981 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
    14041 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
    14101 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
    14161 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
    14221 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
    14281 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
    14341 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
    14401 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
    14461 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
    14521 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
    14581 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
    14641 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
    14701 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
    14761 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    14821 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    14881 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    14941 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    15001 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    15061 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    15121 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    15181 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    15241 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    15301 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    15361 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    15421 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    15481 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    15541 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    15601 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    15661 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    15721 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    15781 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    15841 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    15901 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    15961 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    16021 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    16081 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    16141 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    16201 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    16261 tttctctctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    16321 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    16381 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    16441 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    16501 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    16561 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    16621 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    16681 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    16741 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    16801 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    16861 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    16921 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    16981 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    17041 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    17101 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    17161 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    17221 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    17281 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    17341 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    17401 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatcccta
    17461 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    17521 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    17581 aatcggcacc gtgtttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    17641 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    17701 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    17761 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    17821 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    17881 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    17941 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    18001 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    18061 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    18121 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    18181 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    18241 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    18301 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    18361 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    18421 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    18481 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    18541 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    18601 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    18661 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    18721 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    18781 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    18841 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    18901 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    18961 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    19021 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    19081 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    19141 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    19201 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    19261 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    19321 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    19381 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    19441 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    19501 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    19561 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    19621 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    19681 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    19741 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    19801 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    19861 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    19921 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    19981 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    20041 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    20101 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    20161 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    20221 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    20281 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    20341 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    20401 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    20461 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    20521 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    20581 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    20641 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    20701 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    20761 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    20821 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    20881 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    20941 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    21001 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    21061 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    21121 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    21181 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    21241 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    21301 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    21361 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    21421 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    21481 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    21541 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    21601 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    21661 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    21721 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    21781 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    21841 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    21901 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    21961 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    22021 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    22081 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    22141 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    22201 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    22261 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    22321 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    22381 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    22441 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    22501 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    22561 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    22621 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    22681 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    22741 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    22801 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    22861 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    22921 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    22981 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    23041 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    23101 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    23161 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    23221 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    23281 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    23341 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    23401 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    23461 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    23521 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    23581 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    23641 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    23701 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    23761 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    23821 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    23881 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    23941 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    24001 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    24061 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    24121 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    24181 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    24241 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    24301 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    24361 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    24421 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    24481 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    24541 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    24601 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    24661 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    24721 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    24781 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    24841 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    24901 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    24961 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    25021 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    25081 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    25141 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    25201 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    25261 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    25321 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    25381 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    25441 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    25501 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    25561 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    25621 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    25681 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    25741 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    25801 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    25861 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    25921 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    25981 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    26041 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    26101 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    26161 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    26221 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    26281 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    26341 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    26401 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    26461 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    26521 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    26581 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    26641 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    26701 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    26761 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    26821 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    26881 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    26941 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    27001 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    27061 tggtttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    27121 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    27181 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    27241 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    27301 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    27361 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    27421 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    27481 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    27541 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    27601 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    27661 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    27721 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    27781 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    27841 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    27901 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    27961 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    28021 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    28081 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    28141 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    28201 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    28261 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    28321 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    28381 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    28441 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    28501 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    28561 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    28621 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    28681 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    28741 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    28801 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    28861 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    28921 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    28981 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    29041 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    29101 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    29161 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    29221 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    29281 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    29341 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    29401 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    29461 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    29521 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    29581 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    29641 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    29701 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    29761 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    29821 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    29881 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    29941 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    30001 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    30061 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    30121 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    30181 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    30241 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    30301 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    30361 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    30421 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    30481 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    30541 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    30601 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    30661 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    30721 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    30781 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    30841 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    30901 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    30961 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    31021 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    31081 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    31141 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    31201 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    31261 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    31321 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    31381 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    31441 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    31501 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    31561 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    31621 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    31681 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    31741 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    31801 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    31861 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    31921 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    31981 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    32041 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    32101 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    32161 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    32221 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    32281 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    32341 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    32401 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    32461 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    32521 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    32581 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    32641 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    32701 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    32761 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    32821 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    32881 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    32941 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    33001 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    33061 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    33121 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    33181 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    33241 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    33301 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    33361 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    33421 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    33481 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    33541 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    33601 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    33661 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    33721 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    33781 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    33841 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    33901 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    33961 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    34021 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    34081 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    34141 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    34201 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    34261 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    34321 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    34381 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    34441 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    34501 tctaatggat gcattgctct ttctggctcc ttacttgcgc aaaacaacgg ctttgctgat
    34561 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    34621 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    34681 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    34741 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    34801 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    34861 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    34921 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    34981 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    35041 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    35101 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    35161 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    35221 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    35281 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    35341 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    35401 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    35461 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    35521 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    35581 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    35641 cagaaagagg atgggacaaa aaatgaacgc ccaccctctt cttttttctc tttccttctt
    35701 tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct
    35761 tcctgataga atggatggga aaggagcttg atgccatgac aacgaaagac aaggtacttc
    35821 aattattgaa aaagacacca gattttcttt ccggagaaaa gttggcacag caactggaag
    35881 tttcccggac aagtatttgg aaagccatca aagaattgga aaaggcaggc tatcgttttg
    35941 agcacgggcc tacagggtat cgttacctgc cctctgacgt actagatgca aaagaaatcc
    36001 agcaagagct acggtcgttt attcctgatt tgaaagtaca aacgatgttg acttctgaat
    36061 ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga ttctgtttcg aagttatttg
    36121 ttgctgatac acaagaacaa gctcgtggac gttttggacg gccttttttc gctgaacctg
    36181 gacgaggaat ctacatgagt ctcttgctcc atccagacaa acggtttgat gaattgcctc
    36241 aatatacgat catcgcagcc agtgcctgtg tgaaagccat tgaatctgta accggtcgtt
    36301 ctgcttcgat caaatgggtc aatgatatct acatggatgg aaaaaaagtc tgtgggattt
    36361 tatctgaagc agtcagtgat atggaaagcg gcaggatcag tcatatcatc attgggatcg
    36421 ggcttaattt ttctgttcct caaaatcatt ttccagctga tctccaagaa aaagccatct
    36481 ctgtctttcc cgacggacaa gctagtgtca caaggaatca gctaatcagc aaaatctggc
    36541 aagaattgtt cacttatctt tctcacccag aaaaagcgtt ggacttctat aaagaaaaat
    36601 cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg
    36661 caaaagaaat cggtgatcat ggcgaactga tcgtccaaac aaatgaacag aagatgagtt
    36721 tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca ataattatca gatttcacaa
    36781 aaggacacct gatctcatac agtgtccttt tttctgaatt ctcttcaaaa gactctcctt
    36841 attctatcag ctgacagttt ctctatcctc tagaaaaagg tattctaaaa atatctataa
    36901 agcatggtac actttcttta aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca
    36961 cgatcaaaag agatagcagg ccgcttcctt tttcaatctt atagatagac catgggtaac
    37021 gataaaaaca aaaaagaact tgcgacttat tttgccgtaa gttctttttt agctagtgcc
    37081 ttaatctctt aatcccaaca aacgtctcgc atagatcacc tgaccagagt gcatagcagc
    37141 atcatcaata atagaaacga gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc
    37201 aattacttca tctagctcgg aaggttccag ccttcttaag taagaaatag caaaatcagc
    37261 agctcctcgc aagtatccta gcagatcttc tatttgatta gcttggatct gctttgcttg
    37321 ttccagacta tgcttccaat cttgttcgtt atctgctatc gtcatcggaa accggctttc
    37381 ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa
    37441 ttctcgagcg gtatgccaag ccagccaagt cattgatttg atctgcgggg ctctatcttc
    37501 cactggaaaa cgattggcct cctcaataga gacgccttca aacaaacgtt caaaccgatc
    37561 gtatgcccga tccacactct cgcatagcat atctaaataa tccatttatt ttcctccttt
    37621 ttatttatcc tacttacttt ttcctaattc taggatacct acttatatgt tactacacac
    37681 ccttattttt tacactttaa cagtcaccat catcaatagt ttggagattg aaagaaaaag
    37741 atagtgtcga actatcaatt gaacaagaag caacaatatg aacgtttcat taatattctt
    37801 taaataaaca atgatttttc aaacgaacag tttaagacta ggagaaaaag agtataatag
    37861 gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct
    37921 agttgctttg ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg aaacatacat
    37981 tcctaaagac cctgggaata tttataatga tctaactggt aactatggct tgttaggaat
    38041 tgctagtcaa ttccacgtat ttgctaaaaa taagacaact gtgaatgcac atacagacgg
    38101 aaatatagca acacatgaac ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg
    38161 tttattgaac caagaaataa attatataca aaaaactgat tctttcgcta attcttcagg
    38221 catacctaca ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc
    38281 acttcaagat ggtcatccta cagtcaatgg atccaggctt gatcacttaa ctccagatga
    38341 tttttataaa gatcgtgctg ggcatgaata tattgatttc gagcaggaat ttgctaaatt
    38401 gaatgtagca gcaaatgatc ttgcaacgat cactccagct aaaacatata ctgctgcaga
    38461 ttttccagac atgaacaatc ggacaatcga tttaactgat ttatcagata ccgggttttt
    38521 attggtaaat atcgatgcag aagtgttaac gatgaatacg ccacttcaaa tcattaatcc
    38581 aaatgatcaa gtagttgtat ttaatgtcat caacagttct tctgcattga acgtacagtc
    38641 accaatcaaa tacaatgatc gttcaaatca tgaaacagaa gatttttcgg atgcgaatat
    38701 ctcttggaat tttggaaatg agatgactga tttgacaatc agtgctcctt ttcaaggaac
    38761 aatccttgcg cctaatgcta ctatccgcat tactcaaaac caagacggaa cgattatagg
    38821 aacaaatgtg attttagatg cagctaccaa tcgttgggat cctaacgaaa tatttatcag
    38881 cgacaatggt acagatacga cagacacaag cgacagtaca gatacatcta ccacaagtga
    38941 tagctcagat tcatca
//
LOCUS       DACEJZ010000014        37966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEJZ010000014 DACEJZ010000000
VERSION     DACEJZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.014"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(164..1474)
                     /locus_tag="IW656_000694"
     CDS             complement(164..1474)
                     /locus_tag="IW656_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0836422.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1486..2904)
                     /locus_tag="IW656_000695"
     CDS             complement(1486..2904)
                     /locus_tag="IW656_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0836423.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3144..4712)
                     /locus_tag="IW656_000696"
     CDS             complement(3144..4712)
                     /locus_tag="IW656_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0836424.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4716..5126)
                     /locus_tag="IW656_000697"
     CDS             complement(4716..5126)
                     /locus_tag="IW656_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0836425.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5123..6046)
                     /locus_tag="IW656_000698"
     CDS             complement(5123..6046)
                     /locus_tag="IW656_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0836426.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6043..6921)
                     /locus_tag="IW656_000699"
     CDS             complement(6043..6921)
                     /locus_tag="IW656_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0836427.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6908..8245)
                     /locus_tag="IW656_000700"
     CDS             complement(6908..8245)
                     /locus_tag="IW656_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0836428.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8248..9351)
                     /locus_tag="IW656_000701"
     CDS             complement(8248..9351)
                     /locus_tag="IW656_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0836429.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9425..10933)
                     /locus_tag="IW656_000702"
     CDS             complement(9425..10933)
                     /locus_tag="IW656_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0836430.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10899..11591)
                     /locus_tag="IW656_000703"
     CDS             complement(10899..11591)
                     /locus_tag="IW656_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0836431.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11613..12587)
                     /locus_tag="IW656_000704"
     CDS             complement(11613..12587)
                     /locus_tag="IW656_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836432.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12887..13759
                     /locus_tag="IW656_000705"
     CDS             12887..13759
                     /locus_tag="IW656_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0836433.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14217..16415)
                     /locus_tag="IW656_000706"
     CDS             complement(14217..16415)
                     /locus_tag="IW656_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0836434.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16647..17639
                     /locus_tag="IW656_000707"
     CDS             16647..17639
                     /locus_tag="IW656_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0836435.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17699..19120
                     /locus_tag="IW656_000708"
     CDS             17699..19120
                     /locus_tag="IW656_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0836436.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19113..20630
                     /locus_tag="IW656_000709"
     CDS             19113..20630
                     /locus_tag="IW656_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0836437.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERK
                     GHWMDFNAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20669..22063
                     /gene="uxaC"
                     /locus_tag="IW656_000710"
     CDS             20669..22063
                     /gene="uxaC"
                     /locus_tag="IW656_000710"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0836438.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22222..22905
                     /locus_tag="IW656_000711"
     CDS             22222..22905
                     /locus_tag="IW656_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0836439.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22907..23743
                     /locus_tag="IW656_000712"
     CDS             22907..23743
                     /locus_tag="IW656_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0836440.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23754..25508
                     /locus_tag="IW656_000713"
     CDS             23754..25508
                     /locus_tag="IW656_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836441.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25784..26125)
                     /locus_tag="IW656_000714"
     CDS             complement(25784..26125)
                     /locus_tag="IW656_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0836442.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26193..28364)
                     /locus_tag="IW656_000715"
     CDS             complement(26193..28364)
                     /locus_tag="IW656_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0836443.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28575..29432
                     /locus_tag="IW656_000716"
     CDS             28575..29432
                     /locus_tag="IW656_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0836444.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29503..30360)
                     /locus_tag="IW656_000717"
     CDS             complement(29503..30360)
                     /locus_tag="IW656_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0836445.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30345..33047)
                     /locus_tag="IW656_000718"
     CDS             complement(30345..33047)
                     /locus_tag="IW656_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0836446.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33060..34349)
                     /locus_tag="IW656_000719"
     CDS             complement(33060..34349)
                     /locus_tag="IW656_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0836447.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34499..35545
                     /locus_tag="IW656_000720"
     CDS             34499..35545
                     /locus_tag="IW656_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0836448.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35547..37700
                     /locus_tag="IW656_000721"
     CDS             35547..37700
                     /locus_tag="IW656_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0836449.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11213 a         7815 c         6761 g        12177 t
ORIGIN      
        1 ttttaacgac ttagttatct tatcacgtct aaaaacatgt gtcaactctt ttttggaatt
       61 ttcataaaat gtttcaatga agcgcgacaa cgttgtttaa tatatcatta aaaaaaataa
      121 aggtcaagct aaaatattat ttaagcttga ccttattata atttcaacga ttgtcaaaag
      181 tattgtttgc agctgtgttg ccagtttcta tagataaatc acagcttttg aaacataagt
      241 tttgtactgc ttcgattcgc ccgaattctt ttgcagttat ttggcaatga gaaaagcaga
      301 catcttctat aggcttttca ggaaatgcta ctaaatcaaa agctcgggaa ggaagtgagt
      361 aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat gtctttgact ttcgtcattt
      421 gctttgattc aggtatttct tcagccactg cttgccaact ttctggcatt tgagcaaggt
      481 cattgctatt ttttctattg tattcattat gccagtccat tatccaagaa aaaggaaatt
      541 gtacatcaat catattcaga ttttctaccc tgatattttc gatcactccc cctctttctt
      601 ttgaagattt cattcgaata ccacagtctg actgaaaaaa atcgatatcg tgaatatata
      661 cgtcactaac tccagcacta acttcgctac caatagttac accgtatcct gaacggattt
      721 tacatctagc aacctcgatt cttgatgatt ttcgattgac tcttgcacca ttgccatctc
      781 tgccagactt aatagcaata caatcatcac cacaggccaa atcacattca tagacacgca
      841 catttgttga agaatcaata tcaatcccat ctgtacttgg tccatcattg ttgcggatga
      901 ttactttttc tactaataca tcgttagaat aagtgatttg caagttccaa aaacctgatt
      961 tctgaaaaat caaatcacga acaacaacat gttctgcatg atacagtaaa caagcgcgtg
     1021 gacgtttaat agcataatcc gcaatccaac gaagtccctt ttgatcgtat tcagctcttg
     1081 tcccgctttt ttgatctttt ccccaataca gctcccacca atggtctcct tgaccgtcga
     1141 ttattccttt tccttctata agaatatctt tcgccgagag aacattcaaa atcgctgctg
     1201 gccatttcat ttctacgccg gctactcgtg tatcgatttc tggaaatgct tcgatatctg
     1261 ttctcccctt gaggactgca ccttcttcaa agataagatg actcttatct tttaaaaaaa
     1321 gggctccgac taaataaatg ccttttggaa taaatacagg taagtgttct ttcgcacctt
     1381 gatcgattgc tttttgcagt attattgtat catcttgttg agtgtcgttt gttttaaagt
     1441 tcaaagcatt gataatccct ttttttgtcc gcaatacgtt tctccctatc tttcattgat
     1501 ttttttcaat tcttcaccaa taaatacagt ttttgaacga ttcagtgggt aacctactag
     1561 caaaatgatt gcggatacaa ataagcagaa tgctggtaac aagtttgcca tcgtataaat
     1621 cctttcttgt actaaaactg attggatggc tccacctgtt gtagacgatt ggtaaccaat
     1681 gagtgccagc ataaatccgc ctaccgctcc tgcacatgct tgtcctactt ttctcgcaaa
     1741 agaatttact ccatatactg ttccatcttc tcttaagcct gtcttatatt gatgataatc
     1801 aatcacgtca gtaatgaatg cccagaccat caaattgaat aaaccagatc ctaaagtagc
     1861 gataaataaa agaatcagat atacccatgc atttgttata tgaaggaaat acatcagcaa
     1921 gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct tcctttttac caaataattt
     1981 tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt ccgtaagtaa ataaaagagc
     2041 aacagacatt gcttgtttat tatggaagta atcattgaat aaataagtca tattcgtccc
     2101 agctaaaatc tgattgatca caatgaaaat gtcaacaatg actaaaataa tcagtgctcg
     2161 attagctaac aaacctttta ccaactgaga agcagatact ttttcgtttt tgacaactct
     2221 tactctttca gtagtcaatt tacaagttaa catatatgcg acaaatgcta ttgcagcaca
     2281 tccgattgaa atccaaaaga aatgattccc tgaaacgact tgttgtccac tcttgttatg
     2341 ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat ccgccaatcg cagaaccaat
     2401 gcttcggaac gtggacaacg aggtgcggtc attaggattt gagctaattg ctgctgccat
     2461 tgctccatac gggatattga ttgttgagag aaaaatccca taaacaatgt atgaaacaaa
     2521 aatataagca atcttcaccg tcattggaag atgttggacg aatggcaaaa acaaaatgac
     2581 agtcactaag caaaatggat acttcatttt tctgatccaa ggaataaatc ggccagtttc
     2641 tgttaactct gagacatcac ataatcttcc aaccatcaca tcagcaaacg catccaaaat
     2701 tctcgaaatc aagaataacg tacctacgat tgcccctgaa acgccaagaa cattcgtata
     2761 ataaatcatc aagaaactat tgactaaacc taaaataaaa cagttaccta gatcaccgaa
     2821 catgtagcct atcttatcgg aaatcccaaa ttcctttttt tcttttttta ttgtatttgc
     2881 tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc tcttctagta aacgttttca
     2941 aaattataac tctttttttc ttgtttttat attcacaatg ttggcttttt attgcattat
     3001 cttgacagat aaaaaacaaa aaataatcta aaaacattga tgcataagat tgataacttt
     3061 ttttacataa caatatatac aaacaaaaaa attcactaac tagtgaaaat aagttagtga
     3121 acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt gaaaaactca
     3181 aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc gataaaattt
     3241 ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt cttccattct
     3301 tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt gactttcaaa
     3361 aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac tccgttttct
     3421 gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata acaataaata
     3481 taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt attaggcatg
     3541 tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc taacaaatga
     3601 tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc tggtctacat
     3661 tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt cttctttagc
     3721 tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc ttcatttgtc
     3781 tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa ccaatcgtcg
     3841 gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc accgtttaat
     3901 ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac taagctccca
     3961 tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat aggaaaacca
     4021 ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac tgaatgtcct
     4081 cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa atacaaatgt
     4141 ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg accatcaagc
     4201 ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt cggtgtgtcc
     4261 agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag atcatggaac
     4321 aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg acctgtgatc
     4381 tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc acttttgaca
     4441 tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc ccatatactt
     4501 ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt tgtatgtgac
     4561 cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc aaaagaggag
     4621 acaataatgt aataccgctc tttcactctt aatatattag gatctggatt gaatcctctt
     4681 aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct cattcttact
     4741 cgatataaac tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa cgcaataaac
     4801 tgagtgccaa tcaacaatat gaatcctgtt ataaaataca aaccagtggc agttgttaag
     4861 tattttcctt tagcagtcaa aaacaatgct actgctaaaa aagcaagtga aataaacaac
     4921 acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc taaagacaaa
     4981 gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc agacatatta
     5041 cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc gaccattcct
     5101 agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa tgtatccatt
     5161 caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg acagtctgtt
     5221 catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca cctatcttac
     5281 acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag acgtttctag
     5341 tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt tgatttttcg
     5401 gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg atatgacact
     5461 gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca atccagcaat
     5521 attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa aaaggagtac
     5581 catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa ccagatagtc
     5641 gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc tgtcctacct
     5701 tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca ttcgatcctt
     5761 ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga tactcctgtt
     5821 ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta aaattggata
     5881 atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc tttggatggc
     5941 tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa tccgtcgggg
     6001 tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat gctcccttat
     6061 gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg ttaaagaaaa
     6121 agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc cttttctcag
     6181 cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc taagacgagg
     6241 gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata gatttggcac
     6301 atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtattt cgatgtccac
     6361 ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca cccaccagct
     6421 taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg tgtcttccct
     6481 tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc ataacgtttt
     6541 tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc gttcttcaaa
     6601 tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt ctgcaaaaga
     6661 tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg aaaaatctcc
     6721 gccgtgagaa tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg gataagcata
     6781 gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa gcataaaacc
     6841 gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag atggagcacc
     6901 taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca tccatatttt
     6961 ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct aatagaaatg
     7021 ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc gcaaagccaa
     7081 tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga acaccgggaa
     7141 ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt atgaattgtt
     7201 ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg atattcgaaa
     7261 aatggatcct tctgaatgct ggtgtccgtt catctatcgg ataagccttt ttttcccata
     7321 catacggttc tttccctcgc ggaccacaga aataataaag attcagtata aatggacaca
     7381 tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg cctcgacctc
     7441 gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt gaaatggtaa
     7501 tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt ccatggacca
     7561 tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta tcttcagtcc
     7621 ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta atacgcacat
     7681 ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg ttcaagatcg
     7741 tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa cttggtgact
     7801 ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc atcaatttag
     7861 gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat ttttttccat
     7921 ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga gcatagatac
     7981 aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca ggataatctt
     8041 ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg acattgcttt
     8101 ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc ccgcctccgt
     8161 cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca tcaattgatg
     8221 cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca atgcttgcaa
     8281 gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt agtaagcata
     8341 tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt tattcaagtt
     8401 cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat gtgatttctt
     8461 cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt tagccattgc
     8521 gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt ttgtttctgt
     8581 tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg cagccagtgt
     8641 cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta attcataggt
     8701 atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat gggatagacc
     8761 tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa tcagcaaatt
     8821 tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa catcttggta
     8881 atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac ccatatataa
     8941 gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc cttcggatgt
     9001 gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat atttttcttc
     9061 gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt cgatattgta
     9121 ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt cgatattgtc
     9181 ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt ggaacccttt
     9241 aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt catataagcg
     9301 aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca ttatttgctc
     9361 tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt cccaatcgtc
     9421 tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt cgatcgcctc
     9481 cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc tgtcccatga
     9541 tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact tcattgttcg
     9601 tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt tctttgatca
     9661 attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc acttcactag
     9721 gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg ataaattcga
     9781 tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc atcaatttat
     9841 acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat tgtgcttcgt
     9901 cttttacttg agatttggga aatgctagca agccgccaat accatttcct tctgaccaaa
     9961 catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat tccatatcat
    10021 cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca gtgtagattc
    10081 cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact gcaaagtctt
    10141 gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc atatactctt
    10201 ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca tttggtgctc
    10261 caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc gcacgatcgc
    10321 caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta aattgtttag
    10381 ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta tccaaccagt
    10441 cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg ccgtacaagg
    10501 caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa tttttatagt
    10561 cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg cggacagaag
    10621 agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca gaagccaatg
    10681 cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc agttccgtat
    10741 cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca tgatgcaaaa
    10801 tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg ttacttgaac
    10861 tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat tttacgagcg
    10921 ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt ttcttctgtt
    10981 ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc tgtccacgtg
    11041 actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat tgtttggatg
    11101 atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg aagatgtgag
    11161 aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc ataatgagcc
    11221 atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat atacaaagcg
    11281 atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat cccgatggcg
    11341 ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata tgtgaagtac
    11401 gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac tgcaaccatc
    11461 gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa accgccccac
    11521 agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat tttatctaat
    11581 gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc tcctagcttt
    11641 ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt aaacaatccc
    11701 attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat ataggtgtcg
    11761 aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg ctcgaaccct
    11821 aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg acgaattgcc
    11881 ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc tgcagcttca
    11941 tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga ccagcccaac
    12001 tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga tgttagaaaa
    12061 ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc gtcaacattc
    12121 aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg gatgtaaatg
    12181 atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag taaagacaaa
    12241 atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa cgtatttctt
    12301 aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa accgacccaa
    12361 ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac gagtcccccc
    12421 ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat cacatataaa
    12481 aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt ttcttctttt
    12541 ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag cccctcctta
    12601 gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa ttacgtatat
    12661 aatcattttt accgtaagta aaaacgctta catagcaatg atttttgtgc tatacaaaac
    12721 gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt tttttctatc
    12781 tgacacttag cataggaaat gattatatac gtaatctttc accgatgata aattgaacat
    12841 atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga aaaagaaagt
    12901 ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt tgctagcttt
    12961 gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta cacaagaagt
    13021 cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt atcgctacat
    13081 tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta caggtgtcgg
    13141 aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca gaaagtattt
    13201 gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca gcggggggat
    13261 gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact gggccatgat
    13321 cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt tccaagcctt
    13381 gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg gtgtattttt
    13441 caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt tttatgctgt
    13501 tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat ctaaatggcc
    13561 gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag cagaaagtag
    13621 tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg ttattgcaac
    13681 agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag gagcactcgt
    13741 aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt cagaagtggt
    13801 cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt gtgaaatttc
    13861 ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac
    13921 gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag
    13981 caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc
    14041 gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc
    14101 acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg
    14161 ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac
    14221 aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata
    14281 aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc
    14341 tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt
    14401 tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg caacctatca
    14461 gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc
    14521 tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga
    14581 actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt
    14641 acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat
    14701 tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt
    14761 tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa
    14821 cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag tgttttttca
    14881 tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga
    14941 taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac
    15001 cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg
    15061 agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa
    15121 atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca
    15181 atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta
    15241 tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa
    15301 ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta
    15361 agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct
    15421 ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat
    15481 ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc
    15541 gaaaataagt aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc
    15601 ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa
    15661 atccaattat tataatctga tttcagtact atatacgttt tatctttttc tcttatcact
    15721 ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt
    15781 actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca
    15841 ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat
    15901 ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc
    15961 caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg
    16021 gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca
    16081 taattattat catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa
    16141 taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc
    16201 gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta
    16261 acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta
    16321 ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt
    16381 aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat
    16441 aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct
    16501 ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt
    16561 catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa
    16621 agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg
    16681 gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa
    16741 caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa
    16801 aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag
    16861 gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa
    16921 attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac
    16981 gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc
    17041 tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta
    17101 tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat
    17161 gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac
    17221 gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt
    17281 ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac
    17341 tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga
    17401 gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg
    17461 attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag
    17521 aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac
    17581 aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa
    17641 ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat
    17701 gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt
    17761 tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca
    17821 acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat
    17881 gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat
    17941 cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg
    18001 ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac
    18061 agaggcagga atccaattcg tggagactga taatttaaca gatactcccc cacagagcct
    18121 cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac
    18181 catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg
    18241 ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt
    18301 tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt
    18361 tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct
    18421 ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt
    18481 gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa
    18541 tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga
    18601 agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat
    18661 tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg
    18721 tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa
    18781 gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc
    18841 accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa
    18901 gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac
    18961 agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc
    19021 gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc
    19081 agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga
    19141 aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac
    19201 tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg
    19261 cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg
    19321 tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt
    19381 ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga
    19441 accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt
    19501 ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca
    19561 aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag catccttatg
    19621 gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa
    19681 atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc
    19741 ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc
    19801 aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg
    19861 cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg
    19921 gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact
    19981 acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag
    20041 aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga
    20101 tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc
    20161 ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa
    20221 aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag
    20281 ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgat cttgtagctg
    20341 cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga agagggactc
    20401 cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt tttgaacgca
    20461 aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg gaagtaatct
    20521 tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg aaaaatgaac
    20581 aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga ttttcataaa
    20641 acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt tattaaaaga
    20701 cgattgggcg aaaaagcttt accattccta cgcaaaaaac atgcccatta ttgattatca
    20761 ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc tgacagaagc
    20821 ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg tgcctgaaga
    20881 aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc aaaccgtacc
    20941 aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc gtttcttcgg
    21001 tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag caaatgaaat
    21061 tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg tagaagtgat
    21121 ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg ctcatgaaga
    21181 accagatttg aaggttctac cttcattccg tccagataaa gcactcaata ttgaaaaaga
    21241 aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga tcaccaccta
    21301 tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac atggatgtcg
    21361 tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa cagtattaga
    21421 agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa tcgatgctta
    21481 tcgtacagaa acactgatac atttgaccca cttctatcat tctcataatt ggacgatgca
    21541 gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat tagggcccga
    21601 taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa aattattgaa
    21661 ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga atccgaatga
    21721 ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg gaaaactgca
    21781 gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc aattgacaca
    21841 atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt caagaagttt
    21901 cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt taggcgaaat
    21961 ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag ttcaagcgat
    22021 tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta atagtaacag
    22081 taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt tttagagaag
    22141 aagacggacc aaatttgctc cgtcttcttc tcttgctgtt ctgcttcatt gcctataaac
    22201 ctttatgaaa gaagagataa catgcctact atccatattg ccggtgattc tactgcggca
    22261 gaaaaagatg aaaacaagcg tcccgagaca ggttggggag aaaaaatctc tgcttatttt
    22321 tcacccgaga tccagatcaa taaccaagca aagaatggac gcagcaccaa atcatttatc
    22381 caggaaggaa ggttagcccg tctagcttca cagtttcagc cgaatgattt cttatttgta
    22441 caatttggac ataacgacca gaaaatccaa gaagaaagag gtacgcttcc cttcggtagc
    22501 tatttaaaaa atctgcagat attttttgaa tgcgcaaaaa aagcgaacgt ccaaatggtc
    22561 ttactgacat ctgtgacgcg acgagattac ttagaaaacg gaacattgaa tccagatata
    22621 cttggagact atccaaaagc gatgcgtgca tttgctgaga aacaccatat tcctctttta
    22681 gatgtgttct caagatcaca agaattgttc cagaccttct caaaagaaga gacaaaaaaa
    22741 ttctacctgc atcttatacc tgatacttgt aaaaattacc cagaaggact aaaagacaat
    22801 acccacttta gtccagaagg cgctgataaa gttgcccgaa taattgttga attgataaaa
    22861 gaatcccgtc tgcctcttgc taattattta caaaaaggag tttgagatgt ttccattaaa
    22921 tttaggcatc cgtgcccacg atttagatgt tcgaaatcgt gaagaaatcg ttcaaaaagt
    22981 atcacagctt cacttgtccc atatccaatt tgcaccacaa aaagcatttc ctgaacaact
    23041 taaattaact gacatgacac ttgggagtgc tgcctatttt ggtgaatact tccaaaaaaa
    23101 caatatcgaa ctttccattt taggatgcta tatcaaccta tccagcaaaa atcaagaagt
    23161 tcgtgcacaa gcattggcaa ctttcaaaca gcaaattgct ctgtgctctg cctatcaagc
    23221 caagatgatt gctacggaaa ccggtagtgt ctctgtcggt tacacaaaag aaaattttac
    23281 tgaagaagct tatcagatcg ccagaaattc tatcattgaa ttagtcgctc atgcagaaaa
    23341 ttttggcgta actgttgctg ttgaagcagg gatcaatcat ccaatctata actatcagtt
    23401 agctaaacgt ttaatcgatg aagtagcttc accaaattta aaaatcattt tagattgtgc
    23461 gaatttgatg cataaagaaa atcatgctga tcaggaaaaa gtgatccgag gtgccttgga
    23521 caatttacat ggttatatta cagcgcttca tctcaaagac tatatcatgg aaaatggaaa
    23581 gatccgcatg gtacctgttg gcaagggctg tttagatttc cgcccggtac tgcattatat
    23641 caaatatgag cgtcctctaa tgtacgccac tcttgaagct actcctgaga tttatgttcc
    23701 tgaagcaata gaacacttgc agtttattta ccaagattgt taaggagaag attatggagg
    23761 aacgaataca gaaaagttat caaaaatatc atgcaaatgc ttttcagaca ttgctgcatc
    23821 tttatcaggg aaattattgg cgttttgcca tgtcaggact cttttttatt atcaaacatc
    23881 ttccatcttg gttaatgccg atcgctattg ccaatgtaat caatgcagct tcatcgaaag
    23941 attcttctca gttgcaggcc atttatttca acgctttttt gatggcttgt ctgattggtc
    24001 aaaatatatt gacgaattat tttcatgtaa aataccatag cacttcgatc cgtcaagtag
    24061 aagctggact aagagcgacc cttatcaaga aaatccaaga actatctatt ccttatcaaa
    24121 aagaattgca atctggacga atccagtcaa agatcatccg tgatgtagaa gctatccaaa
    24181 cgttgtcctc tcaagtattt gtcagcggat taaatatcgc tgtcaattta ttcgtagctt
    24241 taggtatcac gctttataaa agtcctgttg ttttcatgtt tttcttattg actgttcctc
    24301 tcgccgcatt cgttactctc tttttccgta aaaggatcag acagtccaac tcagacttca
    24361 ggcaagaaat ggaagagact tctgcaaaag tgatcgaagt gatcgaatta gcaccgattg
    24421 ctcgagctca tgggttagaa aaacacgaaa caactcgctt aaataagcat tttactcaaa
    24481 tctatgaaaa aggattccac ttagatatcc tgcaatctgt tttcggatca gtcagctggg
    24541 ctagttttca gatatttcag ctgatttgtt tgattttcac tgggatatta gctttacaag
    24601 ggcgcatcca acccggcgat gtcgtgatgt atcagacgta tttcacgacg atcgtcaatt
    24661 ccgtttctgg aatgatcacg ctgattccta cgatttcaaa aggcatggaa tctatcactt
    24721 ctattggcga actgttgatt gctggcgatg tagaaactta tcacggtaag aaaaaaatcc
    24781 aacatttgga tggaaatttt cgttttgagg atgtttcttt tctttatccg gactcactca
    24841 aaccgattat cgatcatttt tctctagagg tgaaagctgg agaaacaatt gcttttgttg
    24901 ggccatctgg ctctggaaaa tctacactcc ttaacttatt gattggattt atccagccga
    24961 cttcaggaaa aatttttcta gatgatcagc ctttggatga actggatatg cgaagtgtgc
    25021 gaaattattt agccgttgtt cctcaaacca ctttgttgtt ctccgcttcg atcaaagaga
    25081 acatcactta tggcttgaaa aacgtatcaa aagaacgttt agatgaagtc atcgaagctg
    25141 cgcaactttc ttcactgatt gaccagcttc ctgatggtct ggatacctta gtaggcgaac
    25201 atggcaacaa gctgtctggt ggccaaaagc aacgtatctc cattgcacga gcgttgatac
    25261 gtcagccaaa aatcattttg ctagatgaag caacctcagc tttagataac cagtcagaga
    25321 aaaaaatcca gcaagccctg aattacctga cagaaacacc cactactttt attgtggctc
    25381 atcggctttc aacgatcaaa gaggcagata aaatcgttgt gatcgatgaa ggaaaaatcg
    25441 ttgaaatagg tacttacgaa aaactgttag ctcaaaaagg ctatttctac cgcttgaaaa
    25501 atgaatgaca aaaaatagga gctatgacaa gacttttgtc gtagctccta tttctgaata
    25561 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcatcaa acatgagaag
    25621 ctgttttttg aaattgttcc ttgtaactca gagttaaacg gtaggctcac gacctcttgg
    25681 taaatggtgt tcaagagaca gcctatcggc aataagaagc aattttgtca tcgctcaagg
    25741 ccgaacgtct ctttcacacc ctctgttgtt tcataaaata gttttattcg ctgtaaaggt
    25801 cgcgaactgg tgtcatgatg atacggttca agtcattgat gattgtactg aagccttgct
    25861 ctttttgcat caagtcattg attacttctt ctgattggat tttcacagcc atttcttgtg
    25921 ctttcattgc atcttcgtcg ctgaactctt gtccactcat ttgtttttct tgtaattcga
    25981 tttgcatcgc ttggaagtct ttgaacagtt tgtgtgcttc ttcgtttgct tgtactttct
    26041 cgtacgcttc tttcaacgct ttgaattctg gtaattcacg gatctcacgt tcgatttgat
    26101 tcgcgctatc gtagatgttt gacatgttat gccctctttt cttttagtag ttactctcta
    26161 ttgtaacatg gggttattcg ttttcaaagg ggctactctc cttgatattt ccgccagagg
    26221 tctttagccg cttcaccaat tccctgaacg ccttctttca ccatatcgcc gaagtttttc
    26281 agcccttctt ttgcttgttc accatattga ttcaagttat cttgccaatt tccagactct
    26341 ccattagaat ttcctgtgtt tccaacttga tctgcagcta ccacttgtcc tcctgtggca
    26401 taggcatctg ctacactaaa tcctgttttt gaagtatacg gaaggatgcc ttctgccgct
    26461 gatttgaaga ctttcccgac aacatttcca cttgtccctt ccaaatagtg cagcttgctg
    26521 gattcttcgt aacccatcca tgtcgaaatc actacgtctg gcgtgtaacc gatcatccat
    26581 tggtcatttg cttttgtggc atcgaaatta gtttctgtgg ttcctgtttt ccctgctatc
    26641 gtataacctg caggattcgc agcagccgct gttccatttg aaaatgtacc aagaagcatg
    26701 ctggtcattt catcggctgt ttcctttgat atgacctgtt ttggtttttt gtttgtgtta
    26761 tcgacgatca ccgctccagt cgaatcaacg attttcgtga taagatgcgg cgtatacatc
    26821 tgaccatcat tggcaaatgc actgtaagca cccgccatga tcaatggaga tactcctttt
    26881 tccagtccac caagggctaa gccataatac ttatcagatt ttgctaaagg caggccgaat
    26941 tcttctgctt tatcatatcc tttttgtaaa ccgatctcat gcaaaagcca tacagtaggc
    27001 aaattcaaac tttgtgcaac agcttgatac atcggtactt ctccttggta tgtcccgtca
    27061 aaattatgcg catcgtaata actttgcggt tcgtctttca agatactgga aggcttgtat
    27121 ccagcttcca gtgctggtgt atatacgctt aatggtttga tcgttgatcc tggtgaacga
    27181 cgcatctgtg tagcaaagct gaatcctcta aagacatgat ctcctcttcg tcctactagt
    27241 gcttgtacac cacctgtatt cggatctaaa gctactgaag cagattggac catggcacca
    27301 tctgaagcat ttgctgggaa taatgcactg ttttgataag tagcttctaa ttgcttttga
    27361 taatcctgat ctaaagctgt atagatttta tagcctttat tcatgacatc ttcttcatca
    27421 agaccatact cgttaactgc ttcatcgatc actgcatcaa aataataagg ataacgataa
    27481 ccagaatctg aattatcgta tgtgtcatta agatacgcgc tgatatctgt ctggctttct
    27541 gcttgttctt gttcttttgt gatttttccg ttttcttcca ttacacttag tactgtgttt
    27601 ttccgatcat tcgcattttt tgggtgatcg atcggattat aaataccagg acctttcagc
    27661 attcctgcca aagtagcagc ctcagacagc gtaacattcg ttgcatctac tccaaaatac
    27721 tttctggaag cgtcttggac accccatacc ccgttgccaa aataggcatt gttaagatac
    27781 atggtcaaga tttcttcttt gctgtacttc ttttcgatct cgattgccaa aaataattcc
    27841 ttcgcttttc gatcaaacgt ttggtctaat gtcagatacg catttttggc aagctgctga
    27901 gtgatcgtac ttcctccgcc ccctgatgtt ccgccagaca agaccattcg gactgctgca
    27961 cgggcaatcc ctttgatatc aaacccgtga tgctcgtaaa aattcctatc ttctgtcgag
    28021 attactgcat cttggatata gggagaaatc gcgtttaatt ctacatatgt tcctttttgg
    28081 gcgtacagtg tgccagcttc ttcgtttttc ttatcataaa tgactgtcga ttggcttaat
    28141 cctgatttta acgtttcgac atttgcttgt tttgctaaaa taaagaggta gatacttgtc
    28201 actaagacac agatcaaacc aatcagcaaa atcaatttgt tcacatgata ttttttccag
    28261 atgcgctttc gccatcgatg gaattgtccg agatatggtc tgatccaacg ccaaaaactg
    28321 atcaaagcag cttttatctt ttctaaaatg ctttgaaaat ccatttgctt cttctcctgt
    28381 ctatttgatt caatcgtctt attttaccat agtgaaagag tgttttcttc ctgcttaagt
    28441 tgtagttcaa tatgtttttt tacgttagtg caagtagagt aacataagaa tgaacagaaa
    28501 aatgtaagtt ttttcttaaa tatgttttaa aaatggtaca attagtctgc gattgcactg
    28561 aaaggagtac acttgtgaaa atcacgatca ctttacctat gaaccatcct gaaacaacaa
    28621 ttcgcgactt gcttgaaaag gaatggttag ttcctagaaa ggttcgacac tttttgcgta
    28681 tacgtaaaaa cgtttggatc aatcaacaac ctgctttgtt ccatcatgaa gtaaagggca
    28741 atgaccagat cacattgtta ttcgaagaaa cagattataa ttatcaagaa gttcaattag
    28801 gagatgcgaa gcacatccgt gttttatatg aagatgagca tttgattata gtggataagc
    28861 ctgcaggaat gaagactcat ccaaatgaac cgacagaaaa cagtacctta ctcaatcatt
    28921 tagctgctta tctcgacact aaacagcaac gaccttacgt tgtccaccgc ttggacaaag
    28981 aaacaagcgg tgccgtttta tttgcaaaaa atccatttgt cttgccgata cttggaagaa
    29041 tgttggaaca aaaaatgatc tatcggagat atcaagccat cgtatgggga gaaattgcca
    29101 aagatatgac catcaccaat aaaattggac gagatcgcca tgatcggaga aaaagggtcg
    29161 tagatgataa aaatggtcag agcgccttga cacatgtcac tgtcaacaaa gtactttctg
    29221 aacaaacaca agtctattgt gttttggaaa ccggacgtac acaccagatc agagttcatt
    29281 tagctcataa gggacatccg atcgttggag accctctcta ccaaaataga acagctgaac
    29341 gactgatgct ccatgcatta gaactgcaca tgatccatcc atttactcaa gaaaaaatag
    29401 tcggtaaagc tttacctgga ttatgggaat agaaataaaa aagaacagag tcttatcagc
    29461 agataatgat aagactctgt tctttttgtg taatttgtct gcttactttt gatgttcgtg
    29521 ttctgctaac agcaaactac cgataatccc actttcattc ggcagtcccc aacgaacgat
    29581 atattcactt acttgaggtg tttccatata accacccatc aattcaacaa attgctgacg
    29641 gattttttga aggagatgat cctgattcat taccccaccg cctaaaataa tcttttcagg
    29701 tgctaacgtc aacgtatagt tgatcagtgc ttgagcaata taataagcct gaatagccca
    29761 aacaggatga tcttgcggca gatcttgccc tttgatccct gtgcgtgcct caatagacgg
    29821 tcctgctgct aatccttcta aacaatcttt atgataagga caagtacctt cataatggtc
    29881 atctggatgg cgtttcaccc aaatatgtcc catttcagga tgtgcgatcc ctgaaaaaat
    29941 ttcttcattg atcacgacac ctgcaccgat tcctgttccc acggtaaggt aaatacaact
    30001 attttttcct tgtgctgctc cttgcttcaa ttccccatat gcagcagcat tgacatccgt
    30061 tgtccaagca gcaggaatat tgtagcgttc cttgattgtg ccaagaaaat cgaattgtgc
    30121 ccaccctttt ttgggagttg ccaaaacgta tccgtaatta tcttttgttt tgtcgatacc
    30181 aatcgggccg aatgagccaa tcccgattcc cttcaattca taacgatcaa aaaattcaaa
    30241 tacctgatcc aatgtttctt ctggtgttgt ggtagggata cttaattttt ctaaaatttc
    30301 tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc cgccatacat
    30361 tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat tgggacaacc
    30421 gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt gtctttagtt
    30481 atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac tttatcacta
    30541 aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt taaatattgt
    30601 tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa aggaatctgt
    30661 gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag ttcaatgctg
    30721 taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata tccccaatcg
    30781 cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc tgttacttca
    30841 tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg aatattgaca
    30901 aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac atggtttggg
    30961 cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa ctttgtagga
    31021 aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc aaagtcgatt
    31081 tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg tttttctttg
    31141 gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt ttctaaacgc
    31201 tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc tatttccggt
    31261 gtatctacta atacttttac ttcagagttt tctttgttac tggataggat tggctgatca
    31321 cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc aaaaatatgc
    31381 agattattca actttcgttt gtttttgcga tcttccattg taatggtacc atttttttca
    31441 atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat cattgtttcc
    31501 ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga aaatgctggc
    31561 atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac acggaaacgg
    31621 tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc tggtatagtg
    31681 gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa ctcttgatat
    31741 agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt tatttctgct
    31801 tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct gtcttttggg
    31861 aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc aagatacttt
    31921 ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga atcgactgaa
    31981 caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc ataatctaat
    32041 tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg ttctgtaata
    32101 ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg agcagatgct
    32161 gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc ttctactgag
    32221 cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa attagaatga
    32281 ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga tatatctttt
    32341 tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga ggcatattgt
    32401 tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc tactggaatc
    32461 tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt ttgatcaggt
    32521 cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa aacttgatta
    32581 tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc agcttgtttc
    32641 attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc taacatgaca
    32701 ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac gttcgattcc
    32761 gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa ttttccttca
    32821 ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata gtcatctaaa
    32881 ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc aaatagctct
    32941 agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc catgtaccat
    33001 tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta cgatacctcc
    33061 tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat catatttgcc
    33121 caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc ttcgtgcata
    33181 aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt ttcagatttg
    33241 tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt ttctggcgta
    33301 tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt ttctttactc
    33361 aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata tcccaagtaa
    33421 ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt tcccattcca
    33481 tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc gtgattctga
    33541 atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc tttatcatcg
    33601 agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa catattggat
    33661 ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga ccatgtcatt
    33721 cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc ttttcttgtc
    33781 gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt gaacacgtcc
    33841 aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt tcctgtattt
    33901 ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc atattttctt
    33961 tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc gttagcttct
    34021 tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt tttgactaac
    34081 ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata aggacgaact
    34141 tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa aaaggtggtt
    34201 ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc tgtaaaattc
    34261 tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat aaatttctgc
    34321 accgattttg gtatatcttt ataagccact aataaaacct tctttctatt tgatatatct
    34381 ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata tctttttatt
    34441 tactttttca tccattatgg tattatttga atatgatata tcattaagag gtgtttatat
    34501 gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt taactggtga
    34561 attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct ataaagtaag
    34621 cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta tttatcgcgt
    34681 tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg cttctcctac
    34741 aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg ttggtgattt
    34801 tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga tgacgacgct
    34861 tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg gattgattta
    34921 ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat tgaaagaatt
    34981 ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg ttctttccga
    35041 taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac attcaaaaat
    35101 cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt accttggtta
    35161 ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg acaaaagcgc
    35221 gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt ttgtttgtga
    35281 aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt atcatatccc
    35341 tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg ttgacccgcc
    35401 actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag atgctttggt
    35461 cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt cattcatcaa
    35521 aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact agacatgcaa
    35581 cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc gttccttttg
    35641 ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg tttcatcctg
    35701 atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg atgggtgatt
    35761 tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc cttttccatg
    35821 ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg tcgatctgtc
    35881 aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga atcttatcta
    35941 ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc catcagctct
    36001 ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt tcagaagatc
    36061 ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt gatgagttag
    36121 ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca aaaaaacaaa
    36181 ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac ctggaactag
    36241 aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg cagtcctatt
    36301 ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt tatcataatt
    36361 tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt gggcaaaaaa
    36421 tccactatga tacgactagt cgatctataa aacctggtgt tctctataca aataatgggt
    36481 tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta gaaaaatacg
    36541 aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg ccaaaatggc
    36601 tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc attgcagatg
    36661 ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca atggaaaaag
    36721 gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa gattacttga
    36781 catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta gactatgctt
    36841 atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa atgacgaaga
    36901 aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca ggatttatgc
    36961 gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt tggggacaag
    37021 attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat ttttcaggac
    37081 tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag ctgtgcaatc
    37141 agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg agtgagatgg
    37201 ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac tatccttact
    37261 tgttcagtta tataggacaa cctgaaatgg cacagccact gcttaaacag ttgatgactc
    37321 agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga agtatggccg
    37381 cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc ggtgaatatg
    37441 ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga aaggaattga
    37501 cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc aaacggatgg
    37561 acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc ggcacgatct
    37621 cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat ttgccttttt
    37681 ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc aagtaataag
    37741 aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat taccgcagat
    37801 gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc agcgttctga cctgagtatt
    37861 agagagacaa atggcaaaat agttcctcat attttgccat tttttgagct aataccgagg
    37921 gtcagctgat tgaacaccgt ttagtggaga ggtcgtgaaa aagctg
//
LOCUS       DACEJZ010000015        36569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEJZ010000015 DACEJZ010000000
VERSION     DACEJZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.015"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW656_000722"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW656_000722"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0836450.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW656_000723"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW656_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0836451.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW656_000724"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW656_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0836452.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW656_000725"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IW656_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0836453.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW656_000726"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IW656_000726"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0836454.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW656_000727"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IW656_000727"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0836455.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IW656_000728"
     CDS             complement(8054..8524)
                     /locus_tag="IW656_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0836456.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IW656_000729"
     CDS             complement(8570..8995)
                     /locus_tag="IW656_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0836457.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IW656_000730"
     CDS             complement(9062..9331)
                     /locus_tag="IW656_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0836458.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IW656_000731"
     CDS             complement(9342..10937)
                     /locus_tag="IW656_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0836459.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW656_000732"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IW656_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0836460.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IW656_000733"
     CDS             complement(14490..15050)
                     /locus_tag="IW656_000733"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0836461.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IW656_000734"
     CDS             15403..16377
                     /locus_tag="IW656_000734"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0836462.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IW656_000735"
     CDS             16547..17947
                     /locus_tag="IW656_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0836463.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IW656_000736"
     CDS             complement(18025..18747)
                     /locus_tag="IW656_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0836464.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IW656_000737"
     CDS             complement(18826..19332)
                     /locus_tag="IW656_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0836465.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IW656_000738"
     CDS             19455..21254
                     /locus_tag="IW656_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0836466.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IW656_000739"
     CDS             complement(21322..22896)
                     /locus_tag="IW656_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836467.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IW656_000740"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IW656_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0836468.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IW656_000741"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IW656_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0836469.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IW656_000742"
     CDS             complement(24673..25311)
                     /locus_tag="IW656_000742"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0836470.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IW656_000743"
     CDS             complement(25312..25962)
                     /locus_tag="IW656_000743"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0836471.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IW656_000744"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IW656_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0836472.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IW656_000745"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IW656_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0836473.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IW656_000746"
     CDS             complement(29105..29845)
                     /locus_tag="IW656_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0836474.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IW656_000747"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IW656_000747"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0836475.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IW656_000748"
     CDS             complement(31216..32154)
                     /locus_tag="IW656_000748"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0836476.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IW656_000749"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IW656_000749"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0836477.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IW656_000750"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IW656_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0836478.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IW656_000751"
     CDS             complement(35314..35619)
                     /locus_tag="IW656_000751"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0836479.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IW656_000752"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IW656_000752"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0836480.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9887 a         7988 c         6706 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttaac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaata
//
LOCUS       DACEJZ010000016        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEJZ010000016 DACEJZ010000000
VERSION     DACEJZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.016"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW656_000753"
     CDS             <1..1152
                     /locus_tag="IW656_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836481.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW656_000754"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW656_000754"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0836482.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW656_000755"
     CDS             2728..3003
                     /locus_tag="IW656_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0836483.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW656_000756"
     CDS             3009..3776
                     /locus_tag="IW656_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0836484.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW656_000757"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW656_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0836485.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW656_000758"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW656_000758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0836486.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW656_000759"
     CDS             7920..8222
                     /locus_tag="IW656_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0836487.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW656_000760"
     CDS             8225..9388
                     /locus_tag="IW656_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0836488.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW656_000761"
     CDS             9414..12842
                     /locus_tag="IW656_000761"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0836489.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW656_000762"
     CDS             12863..13987
                     /locus_tag="IW656_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836490.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW656_000763"
     CDS             13977..14414
                     /locus_tag="IW656_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0836491.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW656_000764"
     CDS             14398..14736
                     /locus_tag="IW656_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0836492.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW656_000765"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW656_000765"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0836493.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW656_000766"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW656_000766"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0836494.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW656_000767"
     CDS             15893..16936
                     /locus_tag="IW656_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0836495.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW656_000768"
     CDS             17095..17823
                     /locus_tag="IW656_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0836496.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW656_000769"
     CDS             17939..18184
                     /locus_tag="IW656_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0836497.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW656_000770"
     CDS             18181..18399
                     /locus_tag="IW656_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0836498.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW656_000771"
     CDS             18535..19074
                     /locus_tag="IW656_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0836499.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW656_000772"
     CDS             19067..21340
                     /locus_tag="IW656_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0836500.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW656_000773"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW656_000773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0836501.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW656_000774"
     CDS             complement(22513..22767)
                     /locus_tag="IW656_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0836502.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW656_000775"
     CDS             complement(23167..23436)
                     /locus_tag="IW656_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836503.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW656_000776"
     CDS             complement(23906..24361)
                     /locus_tag="IW656_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0836504.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW656_000777"
     CDS             complement(24499..26427)
                     /locus_tag="IW656_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0836505.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW656_000778"
     CDS             complement(26624..27457)
                     /locus_tag="IW656_000778"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0836506.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW656_000779"
     CDS             complement(27649..28353)
                     /locus_tag="IW656_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0836507.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW656_000780"
     CDS             complement(28376..29002)
                     /locus_tag="IW656_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0836508.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW656_000781"
     CDS             29328..31703
                     /locus_tag="IW656_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0836509.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW656_000782"
     CDS             complement(31727..32218)
                     /locus_tag="IW656_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0836510.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW656_000783"
     CDS             32405..33325
                     /locus_tag="IW656_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0836511.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW656_000784"
     CDS             33479..33823
                     /locus_tag="IW656_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0836512.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW656_000785"
     CDS             complement(34013..34513)
                     /locus_tag="IW656_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0836513.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW656_000786"
     tRNA            35041..35124
                     /locus_tag="IW656_000786"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6169 c         7296 g        10198 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEJZ010000017        34871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEJZ010000017 DACEJZ010000000
VERSION     DACEJZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.017"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW656_000787"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW656_000787"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0836514.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW656_000788"
     CDS             complement(937..1518)
                     /locus_tag="IW656_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0836515.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW656_000789"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW656_000789"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0836516.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW656_000790"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW656_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0836517.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW656_000791"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW656_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0836518.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW656_000792"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW656_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0836519.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW656_000793"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW656_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0836520.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW656_000794"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW656_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0836521.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW656_000795"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW656_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0836522.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW656_000796"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW656_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0836523.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW656_000797"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW656_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0836524.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW656_000798"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW656_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0836525.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW656_000799"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW656_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0836526.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW656_000800"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW656_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0836527.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW656_000801"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW656_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0836528.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW656_000802"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW656_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0836529.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW656_000803"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW656_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0836530.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW656_000804"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW656_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0836531.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW656_000805"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW656_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0836532.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW656_000806"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW656_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0836533.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW656_000807"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW656_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0836534.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW656_000808"
     CDS             13972..14157
                     /locus_tag="IW656_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0836535.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW656_000809"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW656_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0836536.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW656_000810"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW656_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0836537.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW656_000811"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW656_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0836538.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW656_000812"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW656_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0836539.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW656_000813"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW656_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0836540.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW656_000814"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW656_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0836541.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW656_000815"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW656_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0836542.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW656_000816"
     CDS             18232..18879
                     /locus_tag="IW656_000816"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0836543.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW656_000817"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW656_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0836544.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW656_000818"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW656_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0836545.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW656_000819"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW656_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0836546.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW656_000820"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW656_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0836547.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW656_000821"
     CDS             20317..21255
                     /locus_tag="IW656_000821"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0836548.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW656_000822"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW656_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0836549.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW656_000823"
     CDS             21935..22774
                     /locus_tag="IW656_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0836550.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW656_000824"
     CDS             22750..23619
                     /locus_tag="IW656_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0836551.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW656_000825"
     CDS             23609..24406
                     /locus_tag="IW656_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0836552.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW656_000826"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW656_000826"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0836553.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IW656_000827"
     CDS             complement(25688..28357)
                     /locus_tag="IW656_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0836554.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IW656_000828"
     CDS             complement(28595..28783)
                     /locus_tag="IW656_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0836555.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IW656_000829"
     CDS             29053..29415
                     /locus_tag="IW656_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0836556.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IW656_000830"
     CDS             29467..31149
                     /locus_tag="IW656_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0836557.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IW656_000831"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IW656_000831"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0836558.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IW656_000832"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IW656_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0836559.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IW656_000833"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IW656_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0836560.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        11383 a         6400 c         7493 g         9595 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattgttt a
//
LOCUS       DACEJZ010000018        34520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEJZ010000018 DACEJZ010000000
VERSION     DACEJZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.018"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..306)
                     /locus_tag="IW656_000834"
     CDS             complement(133..306)
                     /locus_tag="IW656_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836561.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IW656_000835"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IW656_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IW656_000836"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IW656_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0836562.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IW656_000837"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IW656_000837"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0836563.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IW656_000838"
     CDS             5704..6489
                     /locus_tag="IW656_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0836564.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IW656_000839"
     CDS             6502..7353
                     /locus_tag="IW656_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0836565.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IW656_000840"
     CDS             complement(7477..7929)
                     /locus_tag="IW656_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0836566.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IW656_000841"
     CDS             complement(8394..8762)
                     /locus_tag="IW656_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0836567.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IW656_000842"
     CDS             8912..9394
                     /locus_tag="IW656_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0836568.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IW656_000843"
     CDS             9431..10441
                     /locus_tag="IW656_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0836569.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IW656_000844"
     CDS             complement(10556..11269)
                     /locus_tag="IW656_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836570.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IW656_000845"
     CDS             complement(11459..12049)
                     /locus_tag="IW656_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0836571.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IW656_000846"
     CDS             12533..12760
                     /locus_tag="IW656_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0836572.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IW656_000847"
     CDS             12776..13942
                     /locus_tag="IW656_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0836573.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IW656_000848"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IW656_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0836574.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IW656_000849"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IW656_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0836575.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IW656_000850"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IW656_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0836576.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW656_000851"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW656_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0836577.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IW656_000852"
     CDS             complement(19155..19820)
                     /locus_tag="IW656_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0836578.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IW656_000853"
     CDS             19796..19969
                     /locus_tag="IW656_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0836579.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IW656_000854"
     CDS             complement(20014..20751)
                     /locus_tag="IW656_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836580.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IW656_000855"
     CDS             complement(20753..22255)
                     /locus_tag="IW656_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0836581.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IW656_000856"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IW656_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0836582.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IW656_000857"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IW656_000857"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0836583.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IW656_000858"
     CDS             27597..28862
                     /locus_tag="IW656_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0836584.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IW656_000859"
     CDS             28866..29597
                     /locus_tag="IW656_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0836585.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IW656_000860"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IW656_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0836586.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IW656_000861"
     CDS             30613..31611
                     /locus_tag="IW656_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0836587.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IW656_000862"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IW656_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0836588.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IW656_000863"
     CDS             33123..33773
                     /locus_tag="IW656_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836589.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34520
                     /locus_tag="IW656_000864"
     CDS             33960..>34520
                     /locus_tag="IW656_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0836590.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11452 a         5832 c         7323 g         9913 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata
//
LOCUS       DACEJZ010000019        34506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEJZ010000019 DACEJZ010000000
VERSION     DACEJZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.019"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(178..2070)
                     /locus_tag="IW656_000865"
     CDS             complement(178..2070)
                     /locus_tag="IW656_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836591.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="IW656_000866"
     CDS             complement(2067..3794)
                     /locus_tag="IW656_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836592.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="IW656_000867"
     CDS             complement(3810..4460)
                     /locus_tag="IW656_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0836593.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="IW656_000868"
                     /pseudo
     CDS             4664..4960
                     /locus_tag="IW656_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5132..5752
                     /locus_tag="IW656_000869"
     CDS             5132..5752
                     /locus_tag="IW656_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0836594.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="IW656_000870"
     CDS             complement(5844..7010)
                     /locus_tag="IW656_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0836595.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="IW656_000871"
     CDS             complement(7022..7375)
                     /locus_tag="IW656_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0836596.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="IW656_000872"
     CDS             7503..8525
                     /locus_tag="IW656_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0836597.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8964..9398)
                     /locus_tag="IW656_000873"
     CDS             complement(8964..9398)
                     /locus_tag="IW656_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836598.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="IW656_000874"
     CDS             complement(9508..11367)
                     /locus_tag="IW656_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0836599.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="IW656_000875"
     CDS             complement(11485..12645)
                     /locus_tag="IW656_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0836600.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IW656_000876"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IW656_000876"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0836601.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="IW656_000877"
     CDS             complement(14392..15090)
                     /locus_tag="IW656_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0836602.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="IW656_000878"
     CDS             complement(15047..16708)
                     /locus_tag="IW656_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0836603.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="IW656_000879"
     CDS             16926..18326
                     /locus_tag="IW656_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0836604.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="IW656_000880"
     CDS             complement(18578..19657)
                     /locus_tag="IW656_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0836605.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="IW656_000881"
     CDS             complement(19743..20702)
                     /locus_tag="IW656_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0836606.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22237)
                     /locus_tag="IW656_000882"
     CDS             complement(20714..22237)
                     /locus_tag="IW656_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0836607.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="IW656_000883"
     CDS             complement(22247..22810)
                     /locus_tag="IW656_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0836608.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="IW656_000884"
     CDS             complement(22816..23679)
                     /locus_tag="IW656_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0836609.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="IW656_000885"
     CDS             complement(23693..24589)
                     /locus_tag="IW656_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836610.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26035)
                     /locus_tag="IW656_000886"
     CDS             complement(24764..26035)
                     /locus_tag="IW656_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836611.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="IW656_000887"
     CDS             complement(26025..27542)
                     /locus_tag="IW656_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0836612.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="IW656_000888"
     CDS             27740..28630
                     /locus_tag="IW656_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0836613.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IW656_000889"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IW656_000889"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0836614.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="IW656_000890"
     CDS             complement(29539..31011)
                     /locus_tag="IW656_000890"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0836615.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="IW656_000891"
     CDS             complement(31648..32010)
                     /locus_tag="IW656_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0836616.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="IW656_000892"
     CDS             32233..32985
                     /locus_tag="IW656_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0836617.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="IW656_000893"
     CDS             complement(33125..34048)
                     /locus_tag="IW656_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0836618.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="IW656_000894"
     CDS             complement(34063..34335)
                     /locus_tag="IW656_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836619.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10188 a         7190 c         5935 g        11193 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttaa tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataaca tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atatcagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatt aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcgtgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttctctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttat gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    34501 gtgaat
//
LOCUS       DACEJZ010000020        33250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEJZ010000020 DACEJZ010000000
VERSION     DACEJZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.020"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(324..893)
                     /locus_tag="IW656_000895"
     CDS             complement(324..893)
                     /locus_tag="IW656_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0836620.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IW656_000896"
     CDS             complement(967..2256)
                     /locus_tag="IW656_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836621.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IW656_000897"
     CDS             2610..3458
                     /locus_tag="IW656_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0836622.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IW656_000898"
     CDS             3472..4545
                     /locus_tag="IW656_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0836623.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IW656_000899"
     CDS             4535..6706
                     /locus_tag="IW656_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ0836624.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IW656_000900"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IW656_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0836625.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="IW656_000901"
     CDS             complement(9009..11195)
                     /locus_tag="IW656_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0836626.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="IW656_000902"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="IW656_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0836627.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="IW656_000903"
     CDS             complement(11417..11857)
                     /locus_tag="IW656_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0836628.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="IW656_000904"
     CDS             complement(11931..12404)
                     /locus_tag="IW656_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ0836629.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="IW656_000905"
     CDS             complement(12714..14180)
                     /locus_tag="IW656_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0836630.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="IW656_000906"
     CDS             complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="IW656_000906"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0836631.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16717..16905)
                     /locus_tag="IW656_000907"
                     /pseudo
     CDS             complement(16717..16905)
                     /locus_tag="IW656_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17099..17458)
                     /locus_tag="IW656_000908"
     CDS             complement(17099..17458)
                     /locus_tag="IW656_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0836632.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17488..17829)
                     /locus_tag="IW656_000909"
     CDS             complement(17488..17829)
                     /locus_tag="IW656_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836633.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17826..18500)
                     /locus_tag="IW656_000910"
     CDS             complement(17826..18500)
                     /locus_tag="IW656_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0836634.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="IW656_000911"
     CDS             complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="IW656_000911"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0836635.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20633..21823)
                     /locus_tag="IW656_000912"
     CDS             complement(20633..21823)
                     /locus_tag="IW656_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0836636.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21844..22371)
                     /locus_tag="IW656_000913"
     CDS             complement(21844..22371)
                     /locus_tag="IW656_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0836637.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22418..25207)
                     /locus_tag="IW656_000914"
     CDS             complement(22418..25207)
                     /locus_tag="IW656_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0836638.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25354..25554)
                     /locus_tag="IW656_000915"
     CDS             complement(25354..25554)
                     /locus_tag="IW656_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0836639.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26006..26326)
                     /locus_tag="IW656_000916"
     CDS             complement(26006..26326)
                     /locus_tag="IW656_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836640.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(26627..27745)
                     /locus_tag="IW656_000917"
     CDS             complement(26627..27745)
                     /locus_tag="IW656_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836641.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            28216..29523
                     /locus_tag="IW656_000918"
     CDS             28216..29523
                     /locus_tag="IW656_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0836642.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            29642..30841
                     /locus_tag="IW656_000919"
     CDS             29642..30841
                     /locus_tag="IW656_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0836643.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(30868..32136)
                     /locus_tag="IW656_000920"
     CDS             complement(30868..32136)
                     /locus_tag="IW656_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0836644.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(32299..32946)
                     /locus_tag="IW656_000921"
     CDS             complement(32299..32946)
                     /locus_tag="IW656_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0836645.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         9501 a         7019 c         5859 g        10871 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtgt gtattctttt
    14281 atttaataca catcgttttt tcatattttt tccatcaaaa aaataagtta ttttctttta
    14341 tttcattgac cttattgaat ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta
    14401 gttattttat aatcctatct attttcttta catttttcat taagaaaagg atataaaaaa
    14461 aagaggacgt tcattcgtcc tcttcattga tcaacgttta ttttttacat gcaagcttcg
    14521 catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg catctcccat
    14581 tgatgcaaaa ccaaaagcac ctaaggaaag taccaaaatt tttatcccaa tagcaaagac
    14641 aatgttctgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt
    14701 cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat cgcttcccaa
    14761 tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt tgattccatc
    14821 tccgacaaat gcggtctttt tattgttact ttgtaagatt tcctctaacc gctgcacttt
    14881 atcttctgga agcaattcgc tatatatctt gtccacgcct acttgcttcc cgatatgatc
    14941 agcaattttt ttcgagtcgc ccgtcaacat gactgtgttt ttcacaccag cctgttttaa
    15001 ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa gataaccact
    15061 gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga
    15121 gatttcaaat ctttccatca atttgtgatt acctactaat acttgtttgc catcaacttc
    15181 agctaagatc ccttcacctg caaattcctg gatattagct gttggtaaaa gcggacctgc
    15241 gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg cacttgcaac
    15301 atattgcata aatttctttg gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc
    15361 acctttcgtt aatgtgccag ttttatcgaa taccactgtc tcagtttgtg caagcaactc
    15421 caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa
    15481 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
    15541 tgctcgatag atccattcag aaaacgccgc tctagaaaca acaagtggag gtaaaacagc
    15601 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
    15661 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
    15721 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
    15781 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
    15841 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
    15901 aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg tcatcaatcc
    15961 ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
    16021 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
    16081 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
    16141 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
    16201 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
    16261 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
    16321 ctttttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
    16381 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
    16441 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
    16501 gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
    16561 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact
    16621 aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc
    16681 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
    16741 cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
    16801 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
    16861 tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac tacgctttca
    16921 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca
    16981 tataaataaa agacgtaaaa gtcacataga agaacttata caggaaaacg catagagtca
    17041 ggtgaaatac atgtatccca cctggctcta tgctttatct aacctttatt attctctttt
    17101 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
    17161 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
    17221 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
    17281 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
    17341 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
    17401 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
    17461 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
    17521 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
    17581 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc
    17641 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
    17701 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
    17761 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccttat
    17821 ttccatcatt tttcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
    17881 acaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
    17941 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatacca
    18001 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
    18061 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
    18121 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
    18181 gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
    18241 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
    18301 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
    18361 tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
    18421 ttacctagat cagttgcttc tgctcggtat cttcctgcgt ctgtaatgct atcaccgata
    18481 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
    18541 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
    18601 caccagccta gttccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg
    18661 ctattttttt cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga
    18721 tcgatcacac ccaagcttcc gtcaatcaga ccactgatga cgccatcgaa acatatattt
    18781 ataggctcat ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt
    18841 gggatagatt ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca
    18901 tatggattat tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt
    18961 tgttttcagg tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt
    19021 ccatttgaat taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg
    19081 tgaatcaaat agcaatttct tttgctttct tctcttgtct gctggaaact tccattatcc
    19141 cgtatttaaa aaaaagaact gcgagagttc gcagttcttt tttttaaata aacgttgctc
    19201 tccggccaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
    19261 gtgactcgga gctgaacggc aggcgcacaa cctcttatgg ataaattcgg ttcaacaaac
    19321 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    19381 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    19441 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    19501 ccgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    19561 cacaaattac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    19621 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    19681 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    19741 ttgcatcatc gtaagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    19801 tgtctcgtcc tagtacatct aatgcgtatt cgcaatgatc taaaacattt tgtactaaga
    19861 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    19921 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    19981 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    20041 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    20101 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    20161 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    20221 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    20281 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    20341 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    20401 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    20461 ggaaaaaggc tgttccgtct cttaattgta aaaatgtgat ttttccgctt gatcgcttat
    20521 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    20581 gaatttgttc cacgtatgac tctcctttta tattccttga atttcttcag gattaatttt
    20641 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    20701 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    20761 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    20821 tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc tccttgcggc ttttcaagag
    20881 aaacacctgg cagttctgcc acaagcggat ataacgtatt caggcgttct tcaaaggcgt
    20941 tccgcatttc ttctactgta tcttgctcac ctttcaatgc ttcaacagct gcatattgac
    21001 tgactgctgt cggattactg gttgactggg aagcaacagc aatcattcca ttgatgatct
    21061 cagaattccc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    21121 cattgatgat gatggtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    21181 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    21241 ttactgccca ttcaccgatt gcctgaagtt cattcttgct ataaatcatt ccagtcggat
    21301 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    21361 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    21421 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    21481 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    21541 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    21601 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    21661 cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    21721 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    21781 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    21841 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    21901 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    21961 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    22021 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    22081 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    22141 ctttcgagta aaccaataaa aatgatcggt ttcctcaaga tttgcatatt tctttgcgat
    22201 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    22261 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    22321 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    22381 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    22441 ttaagaattc atggatatcc tgatagattt cctccatagc tagctctttg atcggtactt
    22501 ttttcggtaa tgcatggatg atccgtgcgc catattttgc agtgactagt cttcgatcca
    22561 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    22621 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    22681 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    22741 ctacaacgat ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc
    22801 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccac
    22861 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    22921 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    22981 cgatcagacc tgtaagtgct gtctctaaat aatcgatgta tgcttgattg ctgacttgat
    23041 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    23101 aggcaatcac tttaagaggt gctgcttcaa tgccaagcct cttagagaga tagtgtcgat
    23161 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    23221 cagtcgacgg caacatagcc gcttggaaat catggagatg aaaaatcatc tgctgcgctg
    23281 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    23341 tcagcaagta ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc
    23401 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    23461 gctgctgaat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    23521 gcagctgatc cagcatctct tttgtcatca tgacttcttc tggatactgt ccgttgggtt
    23581 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    23641 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    23701 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    23761 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    23821 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    23881 gtactaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    23941 aacgaacaaa gtcttgttcg taaaacgact gatttcttgc taaaaaccca gtacctctgt
    24001 gcgcgatatc tgcaaagaat gcatggttca agtttgttaa atgtaattca tccaaatcac
    24061 cggtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    24121 gttctggtgg ttccacaagt gttcctttaa atcgttgcag gtcgatatag tggcgataac
    24181 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatttctt
    24241 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    24301 gtgtagccaa ataactcaat ggcaacagat aaccgattgt tttacccatt cctgtagctg
    24361 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagatc gcattcatca
    24421 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa catcttttct tttgcttgtt
    24481 tttttcttgg atatgtccgc tcgccgtaat ggacggatgt gaatagttcc acttcttttt
    24541 tctgcaaagc tagcccatcg acgatttcta atgaggaatc aagtggctct ggtttttctt
    24601 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    24661 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    24721 aaagaagttg acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    24781 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    24841 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    24901 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    24961 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    25021 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    25081 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    25141 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    25201 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    25261 tgcagaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    25321 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    25381 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    25441 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    25501 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    25561 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    25621 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    25681 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    25741 gattacataa agtgaaaaaa cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    25801 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    25861 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    25921 tatttctgat agcctatgac agtgaccaga agaacaaggc aaaccaatac gatcaacgag
    25981 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    26041 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    26101 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    26161 cttctgtttc aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca
    26221 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    26281 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat
    26341 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    26401 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    26461 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
    26521 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
    26581 cctcttcctt aatactcagg tcaaaacact gatttcacaa cctcttttaa taacgaagaa
    26641 tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac actttcccgc
    26701 cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca tattctgttt
    26761 caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa tgtgacactt
    26821 ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc ttatctaaga
    26881 gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc ttcgcccctt
    26941 cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg tctgcacaat
    27001 aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaat gcaaataaat
    27061 aaatcggcca ttgttcagga ttttcgaaag aatcctttaa gtacgtatca acagcttggt
    27121 aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca tggaaagtta
    27181 ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca cctgtcagtt
    27241 cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat acttctttta
    27301 cttgtttgtt gctgacctca tacaatttca tggagtcgcg gttcaatccc aataaataat
    27361 aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga tcacttacat
    27421 agtattgatc gtctaccggg atactcaaac gatggatata ggtctggtca gcagttataa
    27481 tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac aaattttcaa
    27541 ttttttcttg gaacggagac cagtcatgtt cggaatattt tttctcaaaa cgtttctttg
    27601 cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga tgggcgacat
    27661 gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc aattgtgtca
    27721 ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca ttcctttgtt
    27781 tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc taaaaaaata
    27841 aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat aatttcctca
    27901 attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta agaaaaagaa
    27961 taaaagaggt ggatcttatg catttaatta tgattttcag tttattaagt attatcagtt
    28021 tatttgcttt cagcgcttcc cgcttattag atgcggaaac acggcctatt cctgtaaaag
    28081 tacgtaaaaa cgaccgctaa acttccataa cgcgcgttct tatcctaaag ttttttcaga
    28141 aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt
    28201 acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc
    28261 attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tacgttcaaa
    28321 gtaaatgcaa aagcagcttt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa
    28381 gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc
    28441 cttgatcaag taaaagaagg aaaactgtca tgggatgata aagtttcgat ttcagattat
    28501 gcagaaaatt tgagtatcac cccagattta tcgaatgttc ctttgcataa agaaaacacc
    28561 tacactgtca aagaattatt cgactctgca atcatccagt cagccaatgc ttcaatggtg
    28621 gccttggcag aaaaaatctc tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa
    28681 ttgaaagatt ggggaatcaa agatgcaaca atcgttaatg catcaggatt gaacaactct
    28741 tatcttggcg aaaaccgacc agaaggcact ggagaaaacg acgaaaatca aatgtctgct
    28801 caagatgtgg ccatcgttgc ccgccatttg atcttggatt tcccagaaat actggatgta
    28861 tcaagtacta cgacccaaat gtttggcgaa aatacgcaat caccagtgga aatggtcaac
    28921 tggaactgga tgctcccagg attcgttaat tacaaggaag gcgtggacgg attaaaaact
    28981 ggtacgactg agcttgcagg tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg
    29041 atcatcactg tcgtattgaa tgcggacggc catgcggaaa acccgagtgc ccgcttcaat
    29101 gaaacagacc gtttgatgga ctactgctat gacaattggt cagaaaaaga gctcggaaaa
    29161 gctaacgcaa gtattcctag tctaaaaacc attgatgtaa aagacggaaa agaatcaaca
    29221 gttcctgttg tgcttaaaag ccctgtaaaa gtatgggtac gaaacgatat ggatactggc
    29281 aaattaacga tcacgccaac acttgacaag aagcaagtca ctgacaatga gatccaagca
    29341 cctatggata aaggtacaaa aatcggtacc gctacgatca ccttggcaga cgataaatta
    29401 ggctatttag aagacaacaa tgcaccatca acagagatca ttacggataa aacagtggaa
    29461 aaagcgaata tattcgtgat tggctggcga cgcgtcgcag aatttttcga caacttgttt
    29521 taattgaaca ataagaaaaa catctttcca tcggaaagat gtttttttgc tttttctagt
    29581 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    29641 tatgaatcta accagagatc tgatcttttt agttctctat cttattgggg tattctctat
    29701 cgtttattgg cgaaaacaga agaaggatcc ttccttgttc ttttacggca gtacgtggac
    29761 atttacggta atcttaactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    29821 tttgattcca ttgttgttct ttagtatctt tgctttttct tatttcacag aaaaacgaag
    29881 gctgctgaat ggtcttttat tcaatgtttt cctcatcagt ttcggcatct acttgtttgt
    29941 cttgctgtat gaaacgcaaa atattttcct tggcggactg atcgccttga tcacgattcc
    30001 attgctactt gtccttttat ttggtattta tggattgatt gtttttcttt tttggaacgg
    30061 ggtcacagtt ttacgtagag aatcgcattc attagctaat cttttaacgt tgatactagc
    30121 catttttctt acactgtttc ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg
    30181 gataaatgct ttgtttttct gcgttcccct gatcttgatc tatctattta tcgtattcta
    30241 caactttttg actgtctcat tcttgtatca gttcaaccgg cctcgataca atcaagactt
    30301 catcgttgtg ttaggcgctg ggttgatcaa tggagaaaca gtttctcctc ttctagccaa
    30361 gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc cgtgctacat taaatccgcc
    30421 catcttattg atgtctggcg ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat
    30481 gaaacaatat gccatggaac aaggaatccc tgaaagagac attcttgtcg aaaccaattc
    30541 gacaaccact ttggagaaca tgctctattc aaaggaaatc atggatcagc aaatgaaagg
    30601 gccataccgc gcgatcttta gttccaacaa ttaccacatt tttagagccg gtctttatgc
    30661 gtggcaagcc aaattgaaag caaatggtat tggtgcaaaa acagctttct actacttgcc
    30721 taatgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    30781 catttgcgga ttgcttttcg caggctccgc attcttatcg ctgattactt tacttttcta
    30841 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttca ttctcttaac
    30901 tattggcaaa aagagtatcc cttttttgac cgctacttgc tgggcagaat aaataccgct
    30961 gttgcctgaa cacataaagc tgatcaagca gatcataaag aagtatactg ccgcttgtga
    31021 accgaacaat tcgatcccca tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa
    31081 tactccgata aagcccaatc ctgcaagaaa aggaacagaa acatgaagca aaggcgctag
    31141 tgctgcacca agcgttgcac caatttcaaa caatggtgtc acttctcccc cttgatatcc
    31201 cgcgcctaaa gacaaaactg tgaagaataa cttcccaata aaatcataga catgtgcctg
    31261 tccattaaat gattcttgaa gtaacggaag actcaatccg agataacgtt gtgtttgcaa
    31321 aatcaatgct gctgccacaa ctacagctcc acctatcgta tttcgccaaa caggatgagg
    31381 aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    31441 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    31501 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
    31561 aaacagtgca gcaaagaagc tagggaagat cgcttctgtt cggactttcc cgattgccaa
    31621 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    31681 actgattcca ctgataatca agatttcccg ttctgcttta tccaatcgga acaaacgtgc
    31741 tgtattatct gctaacgatc cccccatttg gactgctgtt ccttcacggc caacagaccc
    31801 cccaaataaa tgggtagtta tggttccaaa cagtgtaagt gtaataagcc gcaacgggat
    31861 tttttcttct ccaccatttc cttgatcgat cactaagttg tttccacgac tagaaagacc
    31921 gccgtgatac gcatagagat aagcaaaaag cgcaccacta actggtagta gataaaggag
    31981 ccaaggatgt gatagtcgga tatctgtcac aactgtcagg ctatttaaga aaaaagcaga
    32041 caagctcccc atgaatatac cgagaatcgc tgtgatcacc agccacttca gcatgtacac
    32101 taaaatgcgc attggactcg gtaattgttt ttccataaaa aattcctcct caataaaaaa
    32161 acgcctacta aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg
    32221 ttagcattta aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt
    32281 ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg
    32341 agccaatttc gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt
    32401 tttcgtatac gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga
    32461 tgttttctga cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat
    32521 cccttttaat ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt
    32581 ccagccgatg tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt
    32641 ttttactttt cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac
    32701 gatttgcttc agcttctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa
    32761 aactgtcgtt aaaaatgtat cgatttctgg cagttcgtga tttaatatgt ctcgaatctt
    32821 ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc
    32881 atttaataac tgttctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg
    32941 ttccatgatt ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat
    33001 cttctgcaaa gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc
    33061 atcttatcat cacccccttt aatgcttatt cttctatgta catgattcat tcgataaata
    33121 aaagtaaacc gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa
    33181 agtcgtgtca aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcggtaa
    33241 taagccgaaa
//
LOCUS       DACEJZ010000021        32449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEJZ010000021 DACEJZ010000000
VERSION     DACEJZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.021"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IW656_000922"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IW656_000922"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0836646.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IW656_000923"
     CDS             1011..2258
                     /locus_tag="IW656_000923"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0836647.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IW656_000924"
     CDS             2334..2678
                     /locus_tag="IW656_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836648.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IW656_000925"
     CDS             complement(2795..3121)
                     /locus_tag="IW656_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836649.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="IW656_000926"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="IW656_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="IW656_000927"
     CDS             4812..5327
                     /locus_tag="IW656_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0836650.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="IW656_000928"
     CDS             complement(5526..5972)
                     /locus_tag="IW656_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0836651.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..6615)
                     /locus_tag="IW656_000929"
                     /pseudo
     CDS             complement(6019..6615)
                     /locus_tag="IW656_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     regulatory      6751..6865
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7002..8102
                     /gene="ribD"
                     /locus_tag="IW656_000930"
     CDS             7002..8102
                     /gene="ribD"
                     /locus_tag="IW656_000930"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAQ0836652.1"
                     /translation="MSDTEYMKLAIKLAKKGAGYVNPNPMVGAVIVKDNRIIGQGYHE
                     IFGGLHAERNALKNCRESPVGATLYVTLEPCCHYGKTPPCTEAIIKSGITSVVVGTLD
                     CNPIVSGKGVKVLEENNIQVVIGILEMECQQLIKVFRKYVTRHIPYVFMKYAMTMDGK
                     IATYTNQSKWISGEKARKQVHQFRHKVTAIMVGVNTVIQDDPLLTCRLENGENPIRIV
                     CDTDLRTPITSKIIKTANDIKTYIATSSIDESKIALYRKCGCEIIYTKKKGNHIDLMN
                     LMQCLGNMQIDSLLLEGGSAMNWSALEQQIVDEVQIYIAPKIFGGSAKSPVSGQGVAF
                     PNDAIMLKPYAFSQVGNDYFIESEVIYPCLQE"
     gene            8087..8722
                     /gene="ribE"
                     /locus_tag="IW656_000931"
     CDS             8087..8722
                     /gene="ribE"
                     /locus_tag="IW656_000931"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAQ0836653.1"
                     /translation="MFTGIIEEIGKVERIQKDTCNCKLSIKASKILEDIHLGDSIAVN
                     GICLTVTCFTRQSFTVDVMNETWNRTALSLLRHGSLVNLERAMPMNGRLGGHIVTGHI
                     DGTGRISSVRRDKNAVWYQINTQREILNLIVEKGSIAIDGISLTVAKVSKADFSVSVI
                     PHTLEQTILKNKQVGDMVNLENDILGKYVQKLIDNSNETELSKELLCRYGF"
     gene            8742..9947
                     /locus_tag="IW656_000932"
     CDS             8742..9947
                     /locus_tag="IW656_000932"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HAQ0836654.1"
                     /translation="MKYQKIKEALQALQEGKLVLVMDDKERENEGDLICSAQFATTEN
                     VNFMATYAKGLICMPMSESLANRLMLSPMVEDNTDNHETAFTVSIDYKDTTTGISAEE
                     RGLTARMCVTENVNPFDFRRPGHMFPLIAKNGGALERNGHTEATVDLLRLAGLKECGL
                     CCEIMNENGKMMRTPDLIQFSQTHHIPTLTIKELQEYRKVYDLLVECVSVVEMPTKYG
                     NFKAHCYINKLNGEHHVALVMGDLNNGNDVLCRVHSECLTGDAFGSLRCDCGQQLDKA
                     MKMIAENGSGVLLYMRQEGRGIGLVNKLKAYHLQDNGMDTLDANLALGFQGDLREYYI
                     GAQILRDLGIKSLHLLTNNPDKVYQLEDYGMKISSRVPIEIEANPYDSFYLKTKKDRM
                     GHILNMEEK"
     gene            9948..10415
                     /locus_tag="IW656_000933"
     CDS             9948..10415
                     /locus_tag="IW656_000933"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAQ0836655.1"
                     /translation="MNTFEGNLVAENIKIGIVVARFNEFITSKLLAGALDNLKRENVG
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGSTSHYDYVCSEVSKGIAQ
                     ISLNTETPVMFGVLTTDTIEQAIERAGSKAGNKGSECAQGAIEMVNLIRALEV"
     gene            10824..12038
                     /locus_tag="IW656_000934"
     CDS             10824..12038
                     /locus_tag="IW656_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0836656.1"
                     /translation="MNDKNFIEELRQKREEYGVTQRKLAVACGISRTYFNQIENGTVV
                     PSAELKQTIEKQIERFNPQEPLFLLIDYFRVRFPTTDALKIIREVLQLKADYMLYEDF
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLDCLT
                     AGGKMKRLDLAINDKAGILDIPKLKEKYKAGECISYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGIEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKTNDDWAWFIGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(12544..12732)
                     /locus_tag="IW656_000935"
     CDS             complement(12544..12732)
                     /locus_tag="IW656_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836657.1"
                     /translation="MAVREYHYLTAQVMAIKEDKSFTRTQIGCRSLLQEKGHGVSVST
                     ARCLSKVLNCFIQLSKSD"
     gene            12823..13032
                     /locus_tag="IW656_000936"
     CDS             12823..13032
                     /locus_tag="IW656_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0836658.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNNKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            13144..14757
                     /locus_tag="IW656_000937"
     CDS             13144..14757
                     /locus_tag="IW656_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0836659.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYSCSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKVGNAERIREKKKKASALKKAENRQNEIDRLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            complement(14778..15674)
                     /locus_tag="IW656_000938"
                     /pseudo
     CDS             complement(14778..15674)
                     /locus_tag="IW656_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(16028..16657)
                     /locus_tag="IW656_000939"
     CDS             complement(16028..16657)
                     /locus_tag="IW656_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0836660.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(16641..17105)
                     /locus_tag="IW656_000940"
     CDS             complement(16641..17105)
                     /locus_tag="IW656_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0836661.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(17592..18860)
                     /locus_tag="IW656_000941"
     CDS             complement(17592..18860)
                     /locus_tag="IW656_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0836662.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(19091..19828)
                     /locus_tag="IW656_000942"
     CDS             complement(19091..19828)
                     /locus_tag="IW656_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0836663.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(19895..20464)
                     /locus_tag="IW656_000943"
     CDS             complement(19895..20464)
                     /locus_tag="IW656_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0836664.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            20592..21464
                     /locus_tag="IW656_000944"
     CDS             20592..21464
                     /locus_tag="IW656_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0836665.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            21806..22678
                     /locus_tag="IW656_000945"
     CDS             21806..22678
                     /locus_tag="IW656_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0836666.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            22675..24633
                     /locus_tag="IW656_000946"
     CDS             22675..24633
                     /locus_tag="IW656_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0836667.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(24884..25735)
                     /locus_tag="IW656_000947"
     CDS             complement(24884..25735)
                     /locus_tag="IW656_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0836668.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            25858..26325
                     /locus_tag="IW656_000948"
     CDS             25858..26325
                     /locus_tag="IW656_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0836669.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(26588..27070)
                     /locus_tag="IW656_000949"
     CDS             complement(26588..27070)
                     /locus_tag="IW656_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0836670.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(27170..28033)
                     /locus_tag="IW656_000950"
     CDS             complement(27170..28033)
                     /locus_tag="IW656_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0836671.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            28631..29479
                     /locus_tag="IW656_000951"
     CDS             28631..29479
                     /locus_tag="IW656_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836672.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(29650..29991)
                     /locus_tag="IW656_000952"
     CDS             complement(29650..29991)
                     /locus_tag="IW656_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0836673.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            30077..30784
                     /locus_tag="IW656_000953"
     CDS             30077..30784
                     /locus_tag="IW656_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0836674.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            30771..31094
                     /locus_tag="IW656_000954"
     CDS             30771..31094
                     /locus_tag="IW656_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0836675.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(31183..32220)
                     /locus_tag="IW656_000955"
     CDS             complement(31183..32220)
                     /locus_tag="IW656_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0836676.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT        10202 a         6208 c         6186 g         9853 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggagtt tcccaaacaa aaggatatta agaaataacc tgtgtttacc
     6661 tgcttgcaaa ttattgtatt tggataaacg agaaaaaaac tttttattga cagatataat
     6721 atcagatatt ataattgtcc gtaattgaat aactatcttc agggcagggt gtaattccct
     6781 accggtggta tagcccacga gccgaaaggc atgatttggt gaaattccaa agccgacagt
     6841 atagtctgga tgaaagaaga tgaaagtaag tttatgcttt ttttgcgtgc tctggaatga
     6901 ttttatattg tttcagagca ttttctttaa tcgcataaat cctcttttgc atgtcttgga
     6961 tagattttat tcaaggcatg tatttttttg gaggtgtaaa aatgagcgat acagaataca
     7021 tgaaattagc aataaaactg gcaaaaaaag gtgctggcta tgtcaatcct aatcctatgg
     7081 ttggggcagt aattgtaaaa gataatcgaa ttataggaca gggataccat gaaatctttg
     7141 gtggattgca tgcagagaga aatgcattaa aaaattgtag ggagtcacct gtaggagcaa
     7201 cactttatgt aacacttgaa ccatgctgtc actatggtaa gacaccacct tgcacagaag
     7261 caattattaa aagtggcatt acaagcgtag tcgtcggaac tttagactgt aatcctattg
     7321 tgtctggaaa aggtgtaaag gtacttgagg aaaacaacat tcaagttgtg ataggaattt
     7381 tagaaatgga gtgtcaacag ttaatcaaag tttttagaaa atatgttaca aggcatattc
     7441 cttatgtttt tatgaaatat gcaatgacaa tggacgggaa aatagcaact tatacaaatc
     7501 aatcaaaatg gataagtggc gaaaaagcga gaaaacaagt acatcaattc aggcataagg
     7561 ttactgcgat catggtagga gtgaataccg ttattcaaga tgacccttta ctgacatgcc
     7621 gattagaaaa cggggaaaat cctattcgta tcgtatgtga tacagattta agaacaccca
     7681 ttacatcaaa aattatcaaa acagcgaatg acattaaaac atatatcgct acttcttcta
     7741 ttgatgaaag taagattgct ctttacagaa aatgtggttg tgaaattatt tatacgaaga
     7801 aaaaaggtaa tcatattgac ttaatgaatt taatgcaatg tttaggaaat atgcagatag
     7861 acagtctact tttagagggt ggaagtgcaa tgaattggag tgctcttgag caacagattg
     7921 ttgatgaggt gcaaatttat atagcaccta aaatatttgg tgggagtgca aaaagccctg
     7981 taagtggtca aggtgttgct tttcctaatg acgccattat gcttaaacca tatgcttttt
     8041 ctcaagtggg aaatgattat ttcatagaaa gcgaagtgat ttatccatgt ttacaggaat
     8101 aatagaagaa atcggtaaag tagaaagaat acagaaagat acttgtaact gtaaactatc
     8161 cattaaagct tcaaaaatat tagaggatat acatttaggc gacagcatag cagtcaatgg
     8221 tatctgcctt acagttactt gtttcactcg acaatctttt acagttgatg tgatgaatga
     8281 aacatggaac agaacggctc ttagcctgtt aagacatgga agccttgtga atttagaaag
     8341 agctatgcct atgaatggca ggttgggcgg tcacattgtt acggggcata ttgatgggac
     8401 aggcagaata tcgtctgtgc gtagagacaa aaatgcggtg tggtatcaaa tcaatacgca
     8461 aagagaaatc ttaaatctaa ttgttgaaaa aggttcgatt gctattgacg gtattagtct
     8521 tactgtcgca aaagtatcga aagcagattt ttcggtatcc gttatccctc atactttgga
     8581 gcaaacaatt ctcaagaata aacaagtcgg ggatatggta aaccttgaaa atgatatatt
     8641 agggaaatat gtgcagaaac tcatagacaa cagcaatgaa acagagttgt cgaaagagtt
     8701 gttatgccga tacggatttt agcagaaagg ataaaggaat tatgaaatat caaaaaataa
     8761 aagaggcatt gcaggcgtta caagagggaa aacttgtttt ggttatggac gataaagaga
     8821 gagaaaatga gggggattta atttgttcgg cacaatttgc gacaacagaa aatgttaatt
     8881 ttatggcgac ttatgcaaaa ggcttaatct gtatgcctat gagtgaaagt ctggctaaca
     8941 ggctcatgct ttcccctatg gttgaagata atacagataa ccatgaaact gcctttacag
     9001 tttctattga ttataaggat actacaacag gtatttctgc cgaagaaaga ggattaacgg
     9061 ctcgtatgtg tgtgactgag aatgtaaatc cctttgactt tcgtagaccg gggcacatgt
     9121 ttcctctgat tgcaaagaac ggaggtgctt tggaaagaaa tggacataca gaagcaacgg
     9181 ttgacctatt gaggttggct ggcttaaaag aatgtggctt atgttgcgaa attatgaatg
     9241 aaaacgggaa aatgatgaga acacctgatt taattcagtt ttctcaaaca caccatatac
     9301 ctaccctcac aattaaagaa ttacaggaat acagaaaagt atatgatttg cttgtagaat
     9361 gtgtttcggt tgtagaaatg cctacaaaat atgggaattt caaagcacat tgttatatca
     9421 ataaattgaa tggagaacat catgttgcat tggttatggg agatttgaat aatggaaacg
     9481 atgtgctatg tcgtgtccac tcagagtgct taacaggaga tgcctttggc tctttacgct
     9541 gtgattgtgg acagcagtta gataaagcaa tgaaaatgat tgcagaaaat ggttctggtg
     9601 tattgcttta tatgcgacaa gagggacgag gtatcgggct tgtcaataaa ctaaaagcct
     9661 atcatttaca ggataacggt atggatacac ttgacgccaa ccttgcatta ggatttcaag
     9721 gcgatttaag agaatattat atcggtgcac agattttaag agatttggga ataaaatcat
     9781 tgcatttact tacaaataat cctgataaag tttatcagtt agaagattat ggaatgaaaa
     9841 tttcaagcag agtaccgatt gaaatagaag caaatcctta tgatagtttt tatctaaaga
     9901 caaagaaaga ccgaatggga cacattttga atatggagga aaaataaatg aacacttttg
     9961 aaggaaattt agtagcagaa aatataaaaa tcggtattgt tgtagcaaga tttaatgagt
    10021 ttataacttc taagctatta gcaggtgcat tagataattt gaaaagagaa aatgttggtg
    10081 aaaaggacat agaggtagct tgggttccgg gagcatttga aatacctttg atagcgtcta
    10141 aaatggcaaa aagtaaaaaa tatgatgcga ttatttgttt gggagcagtt attcgaggta
    10201 gtacaagcca ttatgattat gtgtgtagtg aggtatcaaa aggaattgct caaatcagtt
    10261 taaataccga aacaccagtt atgtttggtg ttcttacgac agatacgatt gaacaggcga
    10321 tagaacgagc tggtagcaaa gcaggaaata aaggctctga atgtgcacag ggagctattg
    10381 aaatggtaaa tctaattcgt gcattagagg tataatatga ttggggatta aaaggtaaag
    10441 cgaaaaagca taataggcgg agtgatccag acacggcggt gggaaaatct cttagatttt
    10501 tccaccgttt ttagtggctg gttcactccg gcagttgagc gagcttgcga gcgaacagta
    10561 agcccccgtt atctaacaag gggggcacaa aaaacaagaa acaatataca ctactaagcg
    10621 gaaaacgctg tatttacaag gtaaaatcgc ttttaatagg gtgttaacta aaaagcactc
    10681 atttaataca taaaaattga ataagacata ggaaagacag ttgatttcaa acgagaaatt
    10741 gattgtcttt tcttttgcgg aaatttaagt ataggcaatt atcaaatatg gtagttgcct
    10801 tttttgattg gaggaattga agaatgaatg ataaaaactt tatagaagaa ttaagacaaa
    10861 agcgtgaaga atatggagta acacaaagaa aacttgctgt tgcctgtggt atcagtcgta
    10921 catattttaa ccagatagag aatgggactg ttgttccctc tgctgaacta aaacaaacga
    10981 tagaaaaaca gattgagcgt ttcaatccgc aagaaccact atttctgttg attgattact
    11041 tccgtgtccg ctttcctacg acagacgcat taaagattat tcgtgaagta ttacaactaa
    11101 aagccgatta tatgctttat gaagattttg gaaaatacgg atatgaaagc aaatatgttt
    11161 taggcgacat caacatcatg tgttccatgc aagagcattt aggtgtttta ttagaactaa
    11221 aaggcagagg gtgtcggcaa atggaaagtt atctattggc acaggaacgc tcatggtatg
    11281 actttatgct ggattgttta acggcaggtg gtaagatgaa acgacttgac ttggcaatca
    11341 atgataaagc aggaatatta gatattccaa agttaaagga aaaatacaag gctggcgaat
    11401 gtatctccta ctttcgtatg cagaaagatt acagcggtac ggaaaaatgt ggtagcgatt
    11461 taccaaagaa tacaggagaa accttatatc tcggttcaac cagtagtgag ttatatatgt
    11521 gtgcctatca gaaaaattac gagcagtatg tcaaaaatgg catagaagtt gaagatacag
    11581 aaatcaagaa ccgttttgaa atacgcatga agaatgaacg agcctattat gcggttgtag
    11641 atttactgac ctatcgggac gctgaacgca ccgctttttc tatcatcaat cattatgtcc
    11701 gctttgttga tagagaagat gataaaccga aaagtcaatg gaaaacaaat gatgattggg
    11761 catggtttat aggggaaaat agagagccga tacgattaac gactaaacca gaaccttata
    11821 ctttacaaaa ggcattacat tggttacaaa gacaggttgc accaaccata aaaatggtac
    11881 aagcattaga cagagaaaat catacaacga tactgaaaga tatgattgag caggcagaac
    11941 tcaaagataa gcataaacac ttattacaat tagaaaaatc aaccatagaa gaacgcatag
    12001 ataccgctgt cccacaagag aatgacggta ttttttaatt tcttcaaaat atctgcaaca
    12061 aaacatcaac ttgtgtacag atatggtacg agaaaaggaa atctaacttt ttttgaaaat
    12121 aggtcatcaa atcaaggttt ccattcccta tatggagtga aagaagaaaa gttttgaaaa
    12181 aatgtcggaa attcaagatg agtggacagt gttatgtgaa agaagacaaa gtatcttcaa
    12241 tttgtattca aacaggtatt agcagttgag tgttgatgtg aaaagaagaa aatattttca
    12301 ttctcaactt aacatttggt agttgtcgtt ccctatatgg agcaaacaaa atctttcatt
    12361 tcatagttgg cagtaacggt attgcatagg tagttagggt gtgaaaagaa atcttttcat
    12421 catcaactta gcagaatgac aatttctttc cctatatggt acaagaaaag aaaaaagttt
    12481 tgaatattgg ttacgctttt actcttttcc aacgcgatat ataggaaaga tgattttctt
    12541 ctttcaatcg ctctttgaca actgaataaa gcaattcaat acctttgaca gacagcgagc
    12601 cgttgaaaca gatacgccat gacctttctc ctgcaaaagt gagcgacaac ctatctgtgt
    12661 tcttgtaaaa gatttgtcct cttttatcgc catgacctgt gctgtcagat aatgatactc
    12721 ccgtacagcc acagcttgag cgttaaaagc gtcgcacgca atgggtacgg ctacataaga
    12781 actatgcaga ggtggaagtc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga
    12841 ttttacagaa agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat
    12901 aacaagtgtg gcattggcaa cagaggaaag acaaagattg ttgtaaaaga gcatttctcc
    12961 gaaaagggaa agacaatgga agaacttcta actgatgtca tgttggaaaa agcaaagcag
    13021 acaatcgcat aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg
    13081 ttatacttta cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg
    13141 acaatgaaac aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa
    13201 ttacagggcg aaagttccag cattaccaca caaagaagta tgttgcgtct atatgcaaaa
    13261 gaacatcatt tgaatgtgat tgatgaatat attgatgacg gctggtcggg aacaaatttt
    13321 gacagaccga gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt
    13381 gtaacaaaag acttatcacg acttggtaga aattatatta tgacaggaca atatacagaa
    13441 ttgtattttc ccagccataa tgtccgttac atagcgattg acgacggtgt ggacagcgaa
    13501 aaaggcgaaa gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat
    13561 acgagccgta aagtgaaatc agcctttaag acgaaatttg cagagggtgc gtattatggg
    13621 gcttatgctc cgttaggata taagaaacac cccgacatca aagggaaact gttggttgat
    13681 gaggaaacaa aatggattgt tgaaaaaatc ttctctctag cctatcaagg ttacggtagt
    13741 gccaaaatca caaaaatact gcgagaagaa aaagtcccga cagcgtcttg gttgaatttt
    13801 acaaggtacg gtacttttgc tcatatcttt gagggaaagc ccgaaagcaa gcgttatgag
    13861 tggacgattg ctcatgtaaa ggcgatattg aaaagtgaag tctatatcgg aaacagcgtt
    13921 cataatcgac aatcaaccgt ttctttcaaa agcaagaaga aagtacgaaa gcccgaaagc
    13981 gaatggtttc gagtagaaaa cacgcacgaa ccgattattg acaaggaagt gttctatcgt
    14041 gtgcaggagc agataaaatc aaggcgtaga cagacaaagg aaaaggcaac gccgatattt
    14101 gcagggcttg tcaagtgtgc ggattgtggc tggtctatga ggtttgcgac aaataaggca
    14161 aataaaacac cgtatagtta ttattcttgt agtttctacg gacagtttgg caaaggttat
    14221 tgttctatgc actatatccg ctatgatgtg ctgtatcaag ctgtattgga acgattgcag
    14281 tattgggcta aggcagtaca gcaggacgaa gaaaaggtat tgaacaaaat acagaaagtc
    14341 ggcaatgcag agcgaatacg ggaaaagaag aaaaaggcaa gtgccttgaa gaaagccgag
    14401 aaccgacaaa atgagattga ccgtttattt gcaaaaatgt atgaagatag agcctgtgag
    14461 aagataacgg aacgaaactt catcatgctg tcggggaaat atcaaaaaga gcagatagaa
    14521 cttgaacagc agataaccaa cctaagagaa gaattaagta aaatggaaca ggatatgata
    14581 ggtgctgaaa agtggattga gttaatcaag gagtattccg taccaaagga actgacagca
    14641 ccgttattaa atgcaatgat agaaaaaatc cttattcacg aagcaacaac gaatgaggag
    14701 aacgaaagaa tacaggaaat agagatatac taccgattta ttggaaaagt agactgataa
    14761 agagggttca tatctttaac taagggaaac tggctgtgga gttcaagaga ttttggtact
    14821 ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt
    14881 ctctctaatg ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag
    14941 gaagacaacc gatccggtct gatttgttgc tttttctttt tactggacac tttttccaac
    15001 ttttccgctt gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg
    15061 atcgactctg cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca
    15121 ctataattcc ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct
    15181 agctcatcta gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga
    15241 agctcttcaa tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga
    15301 agcatccctt gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct
    15361 ccgctccatt tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc
    15421 aaatttaggt cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc
    15481 tctgtttgga ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc
    15541 gatttaccga caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga
    15601 gaatcaatag atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact
    15661 gctaaatttt ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta
    15721 aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac
    15781 gaaataacta tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc
    15841 gaaaaaatgg acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt
    15901 tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc
    15961 ttttttatta ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga
    16021 aaacctttta ttcgaacgta gtgatctttt ctggtgctaa acgaacagac tcgtaacctt
    16081 cttctaccgc ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac
    16141 caaatgcctc tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat
    16201 gatcacgcgc aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat
    16261 cgttcatttc ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat
    16321 ctctgacttc ttttggcatc ttccctgatt cgtaagcttg gttatagatt tcttcgccat
    16381 attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc
    16441 tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca
    16501 caaagcgcca tggttgcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt
    16561 ccagcatttc ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac
    16621 cagtcataat tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct
    16681 gttgttatat tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt
    16741 tctacatcgc tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa
    16801 tgctcgattt tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga
    16861 ggatttctct gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact
    16921 gctccttgat caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct
    16981 ttcagtctca tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc
    17041 tggacgattt tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa
    17101 gacataattt cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa
    17161 taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa
    17221 agtatagaga aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac
    17281 gctaaaacca agattattga ttcttatcaa actatagaag atagctcttc tgcagtagaa
    17341 aattttacac cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag
    17401 aaattggatc agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcacaacc
    17461 ttttttgagt aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa
    17521 aaacatgaaa atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc
    17581 actacctctt tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt
    17641 agccaatctc tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga
    17701 ttaaatagtc gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc
    17761 caaccgattt ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca
    17821 ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc
    17881 ccttcatatt cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat
    17941 aaatgttctc ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct
    18001 gtatcatact gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta
    18061 ggcgcagtgg cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg
    18121 cttttccatg caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc
    18181 gttacgttat cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc
    18241 gtaatccatt gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag
    18301 cttcctttaa tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag
    18361 ccactttcaa cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc
    18421 aaactttgtg cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag
    18481 cgagcataaa aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta
    18541 tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg
    18601 ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt
    18661 ggttcttcgg ttgaaattgt attttctgag acagtagctg actgcaaagt actctctgta
    18721 tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat
    18781 tcttcagcta gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat
    18841 aataaattct ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac
    18901 agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt
    18961 ttgataaatt tgctttttct acataaaaag acgtaaatga agaatattct tctccattca
    19021 cgccttaaat tgatggtcgt ttgaataact atttaacaag taattctctt tggatattat
    19081 ccgttatgga ttatacttcc tgtgtcgtcg gacggataat catgttactg acagacatcc
    19141 ggtctggcgt ttctatcgca aatgctacag cagaagctat atcttcggag gtcaatcccc
    19201 attctttttg cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata
    19261 attcagtttg aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct
    19321 cttgacgtaa tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg
    19381 aatcaggata aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt
    19441 gtttttccat gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat
    19501 caagcatatt ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc
    19561 ctgcattatt gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg
    19621 cttggacttg tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta
    19681 aagattcttt tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc
    19741 ctttttctgc taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga
    19801 caatcacttt ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg
    19861 cttatactca ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga
    19921 cgattttggc agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg
    19981 cattcgctgc aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg
    20041 ctccgatcca agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt
    20101 ttggatcggt tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat
    20161 cttctaaaca gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt
    20221 tccctatact aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat
    20281 catctacttt gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt
    20341 acccagtatt cagcgctgct aaaggctctt cgttttcagc ttggatctga tggatcactt
    20401 caaagagcgg atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg
    20461 tcattttgct ccctcctttt atccttactt tataacggac ggcttatcat gtctaatact
    20521 tataaaacat acttaactat tccttttaac tatacttagt ggtatgcttt tttaaaaaca
    20581 ggagggaaaa aatggatatt cgtatactga cgtatttttt agcagtagct agagaaaaaa
    20641 atatcagccg agcagcaaaa tctctgcata tcacgcaacc tactctctca aaacaactga
    20701 aagatttaga agaagaactt ggtgtgacac ttttcactcg gggctcccga gagatacagt
    20761 tgacggattc tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa
    20821 aaacggcgaa caatttaaca aatgacgcaa tcatctctgg agaaatctac attggcggag
    20881 gcgaaactaa agcaatctct ttcatttctg atagccttca ttcgttgatc aaggcgcatc
    20941 ctgatatcca gtttcattta tatagcggga atgcagatga tatcatagac aaactggata
    21001 gcggattact tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat
    21061 ttcgactgcc agcccaagat cgttggggac ttttgataca tcgaaatcat cctttagcaa
    21121 agcaaaccgt catccatccg caggaactga aaactgttcc tttgatcatt tcccggcaaa
    21181 cgtcagtgga cagtcaacta gctgaatggc taggtttcag catagatcag ttatcgatcg
    21241 tcggaacata taacttattg tacaatgctt ctttgatggt cgataacggg acttttggtg
    21301 cattagctct agatgggatc atcaatactt atgggacgga aaaaatattc atcccactcg
    21361 atccgccatt acaagcttca ctgaatctca tttggaaaaa agaccagcct ctttctcctg
    21421 ctgctcgtaa atttttagat gtcctaacgg agaaatacag ttaagtagag tttttttcct
    21481 tcgctttcag ctttttaaca acgaataaga taagcaaaag atccaacgat taaataacat
    21541 aagtttctta atttatttct tccttttatt tctgataatt ccagtaaatt actataatta
    21601 taaaaaaaga attgacaaaa cataactgtc tatataaaat tgattacgtt aacaataatt
    21661 cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta
    21721 ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa
    21781 ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt
    21841 attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag
    21901 aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga
    21961 aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc
    22021 agaaacgatc attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt
    22081 gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat
    22141 ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca
    22201 atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg
    22261 atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat
    22321 ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga
    22381 tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct
    22441 tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt
    22501 caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa
    22561 agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg
    22621 tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa
    22681 gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct
    22741 gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat
    22801 tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg
    22861 ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca
    22921 tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa
    22981 ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc
    23041 ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta
    23101 tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc
    23161 agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa
    23221 ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg
    23281 atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc
    23341 aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa
    23401 gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga
    23461 aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga tgctagcagc agcttttgct
    23521 tcattcttta ctggtgtgac agaaccactt gaattttcat ttatgttcgt tgcttggcca
    23581 ttatatgtct tgcatgcaat tttcactggg atttctttag cagtgagtgc attcttccat
    23641 tggacagcag gatttgcctt tagtgccgga tttgtcgatt acttcctatc tttgaaaaat
    23701 ccgattgcga atcagccatt gatgttgatc gtccaaggat tagtaacagc agtgatttac
    23761 tactttggct ttaactttgc tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa
    23821 gcagatcttt cagatgacga tactgcaacg actaattcag acaataaata tgcggcacaa
    23881 gcaagcaaaa tctacgctgc attaggcgga gccgacaatg taacttcgat cgataactgt
    23941 actacacgcc ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa
    24001 gccactggcg tacctggtat gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt
    24061 acagaagtac aattcgttgc agatgagatg gcaaaactac atggtggtgc tgcagcacga
    24121 cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg ccggtgaaac gatcgaacaa
    24181 gatatttacg ctatcgcaaa tgggaaactg attccaatca ctgaagtttc agatgatgta
    24241 ttcgcggaaa aaatgatggg tgacggctat gctgtattac cagaaaatgg agaaatcttc
    24301 tctccaatcg ctggtacgat cacaaacatt ttcccaacga aacatgctgt cggaatccaa
    24361 acagatgctg gtatcgaagt cttgcttcat atgggcatca acactgttga cttaaaaggc
    24421 gaaccattca ctttatatgt agaagaaggt caaaaagtcg caagaggtca attgattgct
    24481 ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca cggatatggt cgttgtattc
    24541 acaaatggcg acaaagtcca aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat
    24601 gataaaatcg gctctgtttc caacaaagcc tgagccatcg tcttccccag acttttttat
    24661 cgataaacaa acaggagctg agacaaaaat cttgtctcag ctcctttttc cgaataaaaa
    24721 cggcgttctc ctgccaactc cgcggtaata agtccagcta aacgaaacat atgaagagct
    24781 attttttatt tgactcttct tattacttga tgcagaacag ctttttcaca aaatttttct
    24841 tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata
    24901 tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat
    24961 cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag
    25021 ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt
    25081 caataatttc taactcttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt
    25141 cagttagatt gccggataat tctaagattt ctggagagtc actttctact agtacttttg
    25201 ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat
    25261 aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc
    25321 aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt
    25381 ggtgatctgg cgctaatata ctgatcgagt gatcggcatc tgcaaaactt gtataagcga
    25441 tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat
    25501 ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg
    25561 agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc
    25621 ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga
    25681 attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg
    25741 ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt
    25801 tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg
    25861 aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa
    25921 gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat
    25981 gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa
    26041 ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc
    26101 atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt
    26161 ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa
    26221 gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa
    26281 gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc
    26341 ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc
    26401 cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct
    26461 tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg
    26521 aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc
    26581 tatattatca tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg
    26641 taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc
    26701 ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg
    26761 ctaaagcgct tacaacaaga tacgttcctt ttgggaatgg gatgattcgc gtagtgtttg
    26821 gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt
    26881 tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac
    26941 cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc
    27001 tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat
    27061 actttgccat ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg
    27121 gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac tatttttttg
    27181 ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa
    27241 aagagatctc gggagcttcg actaacccat aatctgaaga aggaagccat tctgcataga
    27301 ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc
    27361 aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag
    27421 caaagccaat catgtgcatc aaactgccct tttcttccaa gcggtctcca tcaaatccat
    27481 aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt
    27541 gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga
    27601 caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt
    27661 ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta
    27721 tgccgtatct actgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa
    27781 aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta
    27841 gaagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc
    27901 ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta
    27961 ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca
    28021 gatctttaag catatttatc ttcctccttg attgcctttt tatcatatta tcaaagaaag
    28081 tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct
    28141 aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt
    28201 tttaccttta gaaaaataaa ttttggggaa gacagattct aagtaaacaa agcggtaaaa
    28261 atatctttta ttcccttagt taatccgctt actacctact gtacaaagca aattgttgtt
    28321 caaactgctg atcagaacgc tgcttggatt tttctttgcg atacatcata cctcttttat
    28381 caattggaac aattatttat ttctaacaaa atcacttcat ttttgtcaga taaataaaaa
    28441 acaaaaaaag ttccatcttt aaaacacgct attcttttac tttttctcac attctctaag
    28501 aaaagaaaag gagaatgaat ttttgacaat tcgagcaaaa gtgattatca aatatgtaaa
    28561 atcagctact tttttcttaa acgtttcttt atactaaata taaataagtg tttcatgaaa
    28621 ggagaactag atgaataatt ggcttgagta ttttcctgaa aacgttttgg aacgcggtta
    28681 cagctatcat ctgcacggtt ttgtccgtca tttgaactat acaagcaagt acttgtccgc
    28741 aacagtttct ggcacggaag attataaggt agtgatcact tgggatgaaa agaccaacat
    28801 gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt
    28861 attcgcttac gaagaacgtc caataaaaaa atccaactac tcactttccg aactctcgtc
    28921 ccttgtttcc tccgctagtt cctctcttgt acgggagctt ttaacagaga ttcttataga
    28981 acatccgcac tttatcgaac gcttcaaagt taagatgcca tttcatgcaa taaattattc
    29041 agataaatta acaactatca tccataaata cgatcatatt atcaagaaaa acaaaaacag
    29101 gaaaacagcg aagttcatca tggagatgcg aaagttcatc caagaagcag ttgaatcgct
    29161 tattcagcaa aatgcctatc ttcccgcatt cgaattgatc aatgaagtga ttgctactct
    29221 cgaaacattc tattgggagc ctgaggatga acgaaccctt cttctgatag aggactgtta
    29281 ttatttatgg aaagaattat tagcagaagc acctcatgca gaaaaaaggc aaatgttctc
    29341 ttggttcgtc tgccaagtcg atcatactga cgcctcttat tcaaaacgct actctataaa
    29401 aatcctgaaa gaagactttc gggaaaaaga attttctaac caaaagaaaa aaatagataa
    29461 aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt aaacgataac
    29521 aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg aatcatgcaa
    29581 aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact attaggtacg
    29641 cgttttgttt tataagtcat gacaaatttc cttcgttgct ggatagattt tccggcattg
    29701 ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccgctg catctgtata
    29761 ttggataaaa ttctgacaac aactttcagg tgtgtcgaac caccctaacc ctagtgcagc
    29821 aatcattgtt gcgcctaatg caggtccttc ctccacactt aaacaataga ccgttgcatc
    29881 gaaaatatca gcttggattt gtagccatgc aagatttttc gcgcctcctc ccaccgaaac
    29941 aatacgtgta aatctgcgac ccgctagttc tttacgtttc aatcccacca ttgacaaatg
    30001 ctcattaaaa atttagaata ttcacaagtt taacgacaat tatgaaaatt tatgtcgtgc
    30061 taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt
    30121 acctaccgtt ttcggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc
    30181 cggatttcat ccactagtcg tcacgttgat gtccctgctg gtctatgctg gttctgccca
    30241 atttatcaca gtcagcatgc ttgctagtca cagcccattg ctttccatcg ttttctcgac
    30301 ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa
    30361 aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact
    30421 gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc
    30481 taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa
    30541 ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg
    30601 gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct
    30661 gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa gcaacttagt
    30721 gattgtagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt
    30781 ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt tctagggtcc
    30841 taccatttgt cttactgaaa aaattcgatt taccgcaacc gttgcttgag tatttaagtt
    30901 ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg
    30961 gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg
    31021 ctattctttc aaaaagctta ttggtgatcg tcttggcagg aattctttcc ctttcgttga
    31081 ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt
    31141 ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc
    31201 actaacaacg acttttccta acatatgtcc tgtttctacg tctttggttg cttgtttcaa
    31261 gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc
    31321 atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata
    31381 gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc
    31441 tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag
    31501 gtgcttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg
    31561 atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc
    31621 tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag
    31681 gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccggatc cgttgatcac
    31741 taagattttt ttcccgcgat tggcattttc ttctggagtt agaccaaatt tttcaaataa
    31801 caattcataa gcggtcaatg aggtcaacgg caaggctgca gcttcttcat ctgaaagatt
    31861 ttttggtgca agagcgacaa tacgttcgtc aactagttgg tattcttgat tgcttcctgc
    31921 tcttgttgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcgtc
    31981 acctacagca acaactttcc cgactccatc aaaacctaag atacgtaaag catttcttat
    32041 cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac
    32101 cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg
    32161 atcatcgatc ggcaaccctt cataaaaacc gactaccttc attaattcct tttctgacat
    32221 acgaattcct ccttagcttc atcaagttgg agcatccata cagtgttcat ttcaatatat
    32281 atagtatgct atttgttcca ccttttttat tcatcataat tattctctta cttaaagtaa
    32341 actatttgca cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt
    32401 ataaacggtg ttctcctacc aactcttcgg tcacaagtca aaatattca
//
LOCUS       DACEJZ010000022        32194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEJZ010000022 DACEJZ010000000
VERSION     DACEJZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.022"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..490
                     /locus_tag="IW656_000956"
     CDS             161..490
                     /locus_tag="IW656_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836677.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            477..818
                     /locus_tag="IW656_000957"
     CDS             477..818
                     /locus_tag="IW656_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0836678.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            932..1171
                     /locus_tag="IW656_000958"
     CDS             932..1171
                     /locus_tag="IW656_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836679.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1238..1477
                     /locus_tag="IW656_000959"
     CDS             1238..1477
                     /locus_tag="IW656_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836680.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            1604..2293
                     /locus_tag="IW656_000960"
     CDS             1604..2293
                     /locus_tag="IW656_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ0836681.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            2299..3465
                     /locus_tag="IW656_000961"
     CDS             2299..3465
                     /locus_tag="IW656_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0836682.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            3547..4218
                     /locus_tag="IW656_000962"
     CDS             3547..4218
                     /locus_tag="IW656_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0836683.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4271..6247
                     /locus_tag="IW656_000963"
     CDS             4271..6247
                     /locus_tag="IW656_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0836684.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            6260..7012
                     /locus_tag="IW656_000964"
     CDS             6260..7012
                     /locus_tag="IW656_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0836685.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7028..7789
                     /locus_tag="IW656_000965"
     CDS             7028..7789
                     /locus_tag="IW656_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836686.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7802..8062
                     /locus_tag="IW656_000966"
     CDS             7802..8062
                     /locus_tag="IW656_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836687.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8059..10149
                     /locus_tag="IW656_000967"
     CDS             8059..10149
                     /locus_tag="IW656_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0836688.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10185..12242)
                     /locus_tag="IW656_000968"
     CDS             complement(10185..12242)
                     /locus_tag="IW656_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0836689.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(12387..12578)
                     /locus_tag="IW656_000969"
     CDS             complement(12387..12578)
                     /locus_tag="IW656_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836690.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12766..13221
                     /locus_tag="IW656_000970"
     CDS             12766..13221
                     /locus_tag="IW656_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836691.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13218..13460
                     /locus_tag="IW656_000971"
     CDS             13218..13460
                     /locus_tag="IW656_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836692.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            13478..13780
                     /locus_tag="IW656_000972"
     CDS             13478..13780
                     /locus_tag="IW656_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836693.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13849..15903
                     /locus_tag="IW656_000973"
     CDS             13849..15903
                     /locus_tag="IW656_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836694.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            15903..18515
                     /locus_tag="IW656_000974"
     CDS             15903..18515
                     /locus_tag="IW656_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0836695.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            18512..20890
                     /locus_tag="IW656_000975"
     CDS             18512..20890
                     /locus_tag="IW656_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836696.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20951..21493
                     /locus_tag="IW656_000976"
     CDS             20951..21493
                     /locus_tag="IW656_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836697.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            21571..21903
                     /locus_tag="IW656_000977"
     CDS             21571..21903
                     /locus_tag="IW656_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836698.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21904..22497
                     /locus_tag="IW656_000978"
     CDS             21904..22497
                     /locus_tag="IW656_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836699.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22519..24543
                     /locus_tag="IW656_000979"
     CDS             22519..24543
                     /locus_tag="IW656_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0836700.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            24543..25742
                     /locus_tag="IW656_000980"
     CDS             24543..25742
                     /locus_tag="IW656_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0836701.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25758..26402
                     /locus_tag="IW656_000981"
     CDS             25758..26402
                     /locus_tag="IW656_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836702.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            26413..27219
                     /locus_tag="IW656_000982"
     CDS             26413..27219
                     /locus_tag="IW656_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836703.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            27242..27471
                     /locus_tag="IW656_000983"
                     /pseudo
     CDS             27242..27471
                     /locus_tag="IW656_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27630..28565
                     /locus_tag="IW656_000984"
     CDS             27630..28565
                     /locus_tag="IW656_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ0836704.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            28609..28878
                     /locus_tag="IW656_000985"
     CDS             28609..28878
                     /locus_tag="IW656_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836705.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            29154..29429
                     /locus_tag="IW656_000986"
     CDS             29154..29429
                     /locus_tag="IW656_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836706.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            29471..29893
                     /locus_tag="IW656_000987"
     CDS             29471..29893
                     /locus_tag="IW656_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836707.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            29913..31505
                     /locus_tag="IW656_000988"
     CDS             29913..31505
                     /locus_tag="IW656_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836708.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            31526..31861
                     /locus_tag="IW656_000989"
     CDS             31526..31861
                     /locus_tag="IW656_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836709.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            32007..>32194
                     /locus_tag="IW656_000990"
     CDS             32007..>32194
                     /locus_tag="IW656_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836710.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11590 a         4964 c         6567 g         9073 t
ORIGIN      
        1 attggatcaa tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa
       61 cgagtcgatt tattttatat atttttgagt gaacgaaaaa cgataaagat attcgttatg
      121 aagtaaaact aatttagtag atagcaaagg tggtagagga atgattcaaa aatttgcgaa
      181 aatgagtaaa actgcatgga ttgaaaagct tgaaaatgac ttgagtgtca tgcgattagc
      241 tccaaagaaa ttcaaacgag agattgcaga aaaagaagca attttgtcgg tattgaatgc
      301 ggtagacgat aaatgtccag taaaaattag tttttcagat atagcaacaa ttatcgaagg
      361 tatttattat ggtgaagttg atgaagagat atcttctaca aaagatttat tagatttttt
      421 ggataaaaac aaagatacct ttatagtact aactgattat gaggaggtta caacaaatga
      481 aaaattatga tccgaatata cgattgggca cacatacgat taaggtttct ttccaacgtt
      541 gggattacaa aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt
      601 tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg
      661 gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt acggatgata
      721 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      781 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      841 agataaaggc ttagaaggag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      901 gagtaaggaa tttatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      961 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
     1021 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
     1081 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
     1141 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
     1201 tttatttttg caaaaaacaa gaaaggtagg aaaactcatg aattatgaag aaatcgaaaa
     1261 cagaaaaaaa gtgtcgaaag aaatggaaga aaagttgcta aaaatgatga aacaaaaaca
     1321 ccttaaaaga ttatctgtca tgcaatatat caatgatatg aagataacag gaaaagagaa
     1381 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg aggcgaactt atgtaaaaac
     1441 aggttccaat tgccaactat tattagaagt ttcttgacga ttatgatatg aaaatgatca
     1501 attatgatgt ggcattgtta ttgaataaaa agaatagtaa aatttaacat ggtttatctt
     1561 ggttagatgt aggtaaatta attcatatat agaaggtaga tagatgaata aaaacaataa
     1621 aactttattt aatccttacg tgggcgtaat cattgttttt cttcttttta gttattttgc
     1681 ttatcaaggt tatcaaaagg acaaaatcgc tgaaaaagta gatggtgtaa caaaaacaat
     1741 ccatattcag gaacaggcta aaacctatca agataattta accagtttaa cggatatttc
     1801 taagagacaa ctgattacta atcaaaaaca ggcattagaa aaggatctca aaggtgaaat
     1861 tgctggaaaa cttgtttttt cttctacagg atttattctc cctgtttttc aaggagcgaa
     1921 tagcgacacg cttagtttag gagtagcttc tacttattat ctagatactg aaatgggaaa
     1981 tggaaattat attcttgctg gttataacgt agatacacca agcgttttat tatccgatat
     2041 gactgattta tctgaaggag aagaaattga tctaatcgat gctcattaca agtatcagta
     2101 tgaagtctcc aaaagaggaa aaatttctga ttacagccag gtagttcaat ctaaggaaaa
     2161 caattccttt ttatccttgc caaaagcagg taaaaaacca ctactcacac tgttagcctt
     2221 tcaagcaaat aatatgaaaa agtgcgatgt gatccagtgt gaattagtca atgtagaaaa
     2281 aatcaataaa taagggaagt gagtcacatt caagaacttg tagaaaaatt gatcaataaa
     2341 atgagtgcca tttttctgtt actgttggta tccattctat atttaatatg gacggtgggg
     2401 ttaaatcctc ctacgatatt atttatttat gataatgctg cacagttagt gacgtttttc
     2461 gtaggctgtt tgtttctttt gaatctgtac aaggtaaaga gaaacgatct attcttcatt
     2521 gcagcaggca ttctttttta ccttttcttt tcttttttta gaagctaccg aggagatact
     2581 ttctatggtg atctgatcgt tccgacaatt attgctatta ttttgattgt aaaatggacg
     2641 aaatttactg taattgatcg tagcttgtat cttgtagtat tctatgtctt tttactcgta
     2701 acggtttatc gagtatttac tgagatacca gtaccagaag gacaaagtat ttggaatcca
     2761 tcgaacaaat tatcagatat ttggatcaat acaaatacga taggtagttc tttaatgaca
     2821 ttagcccttc ttatcacagg atttgctagt tcatttggaa aatggtacat gcgattgcta
     2881 tcattaccag cgttgattct tgctggggca acgatttggg tttgccaatc gaaagcagca
     2941 ttatatgcgc ttgttatctt cattatctta gaaattttac ctaagaaggt ttttcgaaat
     3001 acacacttgt ccttttggat gtatgcagca gtttcggttc tagctttacc gatttcttat
     3061 tttgctgcaa tgtcagacaa agttcactta tttacaggac gagaagaaat ttggcgcaaa
     3121 ttctatgaaa cattaggcga aaaaacagga caaatatttg tagggatgaa acctttcttt
     3181 ttccatagag gaaatcagat tctaggtaat cataatacgt atcacgcact cgtcaatcac
     3241 tatggattga ttggttttgt tctatcgatt attcttttgc ttagtttctt atggttgctc
     3301 gtaagaaaac gtgagctgac aaatggacaa ctaacattct tgtgggcttt tttagcgatc
     3361 atgtgtcaat cgttcatgga agaaacatta acgacaatca gttgggtgcc aattgtattc
     3421 ttaatgttag caatggctac acaccaatat aaaaccacca gataaatgaa atagaatagt
     3481 aataagagaa aacacttttt cgttgtgaaa aagtgttttt tgttttgtct gaaaggaggc
     3541 agatggatga aaaaatggtt atttggtttt ttaggagtcg ctttaatcgt cgtttgcagt
     3601 gtgtttggct atgttagcta ccaaaaacat gaaggagagg tgtttaagca aaatatagaa
     3661 aaaaagatgc ccgtagatca aatcaatgca cacgcaaaga gttataagga ggatgcaacg
     3721 aacgtcaata atgatatgtc tttggggcaa atgttgtcca tacaaaaaga agcaatcgaa
     3781 atgggcgtca ataaacaagt atttgcacaa attcagattc ctgcacttgg tttagcattg
     3841 ccaattttta aaggggcaaa tcaatacact ttgagtcttg gagcggctac ttatttttat
     3901 gaagatgccg aaatgggtaa aggaaactat gtcctagcag gtcataacat ggaaatgcca
     3961 ggtgttttat tttctgatat tcaaaagttg tcattagggg aggtgatgga cttagttagt
     4021 aacgatggcg tctatcgtta caaggtaacg cgtaaattta tcgtacctga gtactttaaa
     4081 ttaatcgatg gcgttcctga agagaactct ttcttgtctt taccaaagaa aggagagaaa
     4141 cccttattaa cgttgtttac ttgtgtttat acgtcacaag gaaaagaaag atacgtggta
     4201 cagggagagc tacaataaca agaatttaat caacgcgagt cgttgaaata tgaaatgagg
     4261 agagtaaaaa atggcaataa atcggtatgt aaaaaaagct ggaatgattg ttggaatgtt
     4321 aggaatcatt gcaggctgct ttcaggggta tcgagcttat gcagaagatg tcacgcaaaa
     4381 aacgccacca gaaagggtca atatcacggt tcataaatta atgtacgatc aaagtacgca
     4441 attgaacgtt gatattgacg gaatcaaaaa tgatggttat acgcacgatc aatatcctac
     4501 aggtgtaacg aaatacaaca aggcagatta tggtgatgtt gagtttacgt taggaaatat
     4561 tacggatcaa gtattaccga ctgaagattc tgatctaacc aacgcgaaag tggacgaaat
     4621 cgttaaagat gtagaagaca agggatctaa cagtgagtac gttaagaatg caaccaataa
     4681 aattacttca gctgtagatg aaaatggtga gatcactttt gctgatcaac cagcctatgt
     4741 caattccaaa gggaatgtat atgtggttta tgaaagtaaa tccgctgctg gattagtcac
     4801 acaaaaagca aaaccaatgg ttgtgattgc gccaatgaca gacaacacag gatcaggatt
     4861 tttaaaagat attcatttgt atccaaagaa cattgtttct aagttgagtt ttgaattaac
     4921 gaaatttggt gacgatggca cagctcaaag caaacaaaca cctttaaaag gggcaaaatt
     4981 tgagttatac aagggcgaac ctggaaaagg aacaaaatta ggagagcttg tatcggatga
     5041 tcaaggaaag ctgactgcaa ctgatttgac gttaggaaaa tactactttg tggaagttcc
     5101 ttcagaagtt gtagttggat cagatcaaga accaacagca gaccaatatt tgttaggcgc
     5161 agacgctcgc aacgatgcgc acaataagtt aacctttgaa attacgaatg acggcgtgac
     5221 aagtgattta aaagcttcct acgtgaacta taaagcacca gtgatcgata aaacagtgac
     5281 aaatggaaca ggagaagaac attctttcca aattggtgac gcagtgaatt atcaaggtac
     5341 aattcatatc cctacagata tcgcaggtgg aacagatggg attacagtaa atggtgtaaa
     5401 aagtgaaact tctccttaca gtgtatttaa atggggcgac acagcaggta aaggactttc
     5461 ttatgttgct gaaaaagcag atattaaagt aacaaataat gatggttcag tcgtattaaa
     5521 agaaaatact gactataaaa tcgaaaattc tgatagtggt tttgtgatcg attttatcgt
     5581 aaataatgga caagtaagtg atacagttgc gaatttacat ggtcaagatc taaaaatgac
     5641 ttataacatg tttgtgaatg atagtgctga aatcgcaaat ccattaacaa atagcgtgga
     5701 tttcgtttat aacaataatc catttaatca ggaagaacac cacgaaaaaa cgaaagctga
     5761 cgttgtgact tatggcgcaa aattcttaaa agtcgatagc ggcctatttg gaacaggtat
     5821 caaagcaaca ccgcttgaag gcgcagaatt tgcagctaag aatgcagaag gtaagtacta
     5881 cggtggactt actgatacag ataaagatgg tgtcaaagaa gcagtttggg tagacgatgt
     5941 agcaaatgca gcaatcctaa aatcagataa agagggtcat tttgaaatca ctggtttaac
     6001 tgaaggagat tactcactgg aagaaacaaa agctcctgaa aactatcaaa aattaacaaa
     6061 agagatctct ttcaaagtag acaaagattc atacaaagaa gaaaaccgaa tcacgatcaa
     6121 aaataatcaa aaagcttccg taccaatgac tggtagcaac ggtttccaat cttacgtatt
     6181 gatctcttgc ttgttgcttg gggcaggcgc aatttctgca gttgtttact ttaagaagaa
     6241 agcctagaag gatcagctaa tgattatcag acaccctaag aaaaagcgaa tcatgggtaa
     6301 atggatcatt gctttctggt tactttcagc tgttggtgtc ttacttttga tgcctgctga
     6361 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     6421 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     6481 tattcttaca attccaagta tttctttaaa actaccaatt tatgacggaa cttccgacaa
     6541 aattttagag aatggtgtag ggattacgga aggaactggc gatattacag gaggaaacgg
     6601 taaaaatcct ttaatcgcag ggcacagtgg actttataaa gataatcttt tcgatgattt
     6661 gccttcagtt aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     6721 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     6781 cttagaacca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     6841 caacacacac cgtttcctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     6901 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     6961 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     7021 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     7081 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     7141 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     7201 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     7261 tcacgttcaa aaaattattt cttccgaaca attagacaac caggggcaaa agcttgaaaa
     7321 acaagaagga gtaaatggcg cgaagttttc tgtttatgat gtatcggaca ttttacaaaa
     7381 gatggatgtt aaggatctca ctactgacca aattgaatcc caattaaaag atcgagtgaa
     7441 aaaactatcc agtgatcaac tgaagctaat ttctaatgga gaaacaaaga caattgatca
     7501 acaaacaggt gtctttgaat tctcggtaga agtacaggca aatcagaaac aagcttatta
     7561 tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc gaggatattt tgctattaac
     7621 acctgtttct gataaaaatg gtgaattctt aaaggatgtg tggatttatc ctaaatcaga
     7681 agcttcgcaa cctaaagaag aagtaaaaaa aatcgtatct actggcgtaa agaaaaactt
     7741 ctttgagaat tgttgggatt ttgttactca cttgttttct agagattaag gaagaaataa
     7801 aatgaaactg atcaaattat tattattcaa tttttcttgt ttaaccgcag tctttttgta
     7861 ctgcggttta ttattttcta aactatctat tttgccaagc taccaactat acctaatgag
     7921 gattttaaac ataccagctg cttccatgcc gctagatatt atggaaaagc tcattaggaa
     7981 tccattattt actacgatca atttgatcgt tattgtcgga atttcactag tcatttataa
     8041 aaaagagggg atgaagggat gaattttaaa cactcgtgga caagacggtt aaaatatgtt
     8101 tttcccataa tgatgatgtt aggtacattg tttgggttaa aaacagccaa tgtctttgct
     8161 gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg taaagaataa ttttattatt
     8221 ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa acggagcgtc aaaagtttac
     8281 aaatatcatg ttgatgccaa caaaggtggt tttacagacg actacgtagg attagtggag
     8341 ggaaacaaaa atatacgcta tccttctttc caaaaagaat acggtgaaac aaaccttgtt
     8401 ttagaaggcg taacaaccaa caccaaagtc aatattgact atggaaaaat cggtacgtat
     8461 aacgggaaaa aagtcaatat caagctagtt ctatctaata ttcacttata ctcggatacg
     8521 ttgccgtgga atctactaga taacaactat acaaaaactc attttcgaga cgatggctat
     8581 aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa cagtactttg gatctcagac
     8641 aatcttttct caggaattgt ttaccattca acacaaatga atgtgcagct agtagccaca
     8701 tatgaggacg gtagccccgt tcaattttca ggagatacat ttatcagttt taactctttg
     8761 aaccctgcag gaggaaaaag cacggatctt aaaggagaat atgcccacta tgacaaaatg
     8821 aatacgacag attggtatgt cagaaaagat acggttcttt cagaatttaa atctttctac
     8881 aacaatctga acgtggtagg tggccaccca ggaggaagct ctaagctgac acaagcggat
     8941 aatgatttca ataatttgca tgataaatta ggagatccga agtttggtca aggtactgtg
     9001 tcctttaaaa tatctgaagc aaatccgacg tttgtgattg gttcttctaa tgttcaaacc
     9061 tggtttactt tatcttctgc gacaatcttt agtgttgttc ctgatcaacc tgaaaaaaca
     9121 ggggttgata aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg agacacgatt
     9181 caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg atttactagc ggtttacgat
     9241 cgatttgagt taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc acacgttgaa
     9301 agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa aacaaaacac
     9361 caggtaaaat atgcagcgaa agctgataca ttaaaaaatc gaatgaagta taacggcgaa
     9421 acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa tagtgtagcc
     9481 aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg atacaaatat cattaccgtt
     9541 tatttcccta aaattccagt gaaagaagta caacaaaatg gaaaagatgt aaacggacga
     9601 aatgacggta agaagggtac gccgacagct cctttaaacg caggatctga agtacaatat
     9661 ctagtcacac aaaaatggca cacaaaaggc gtagacgctg cgtcagatca ctacaaacaa
     9721 tttagtattc aagatccaat tgaagctcgt ttgacatata aagaaggttc tgcgcaagta
     9781 atagataaga gtaaaggaaa agatattact tctgaaggca cactaaccta tgatagcaat
     9841 tcaagaacgc tgaagtggga agcttccgct gacttcttaa ataacaatct tttagatgga
     9901 cgagaaattc agctgatatt tacagctaaa actccattac aatcagaaaa aaatattgat
     9961 aaccaagccg tagtagcagt agataatgtt tctaataaaa caaatgttgt gacaattggg
    10021 gttgatccta acttgccaca agttattgtt cctaaaacag gttctacaca tttagtaacg
    10081 attttagcgg tcagcttact attacttgtg ttagcaactt ttggctacgc agttctgaag
    10141 ttcaattaaa caaaaaaaca aggctgaaaa tcagccttgt ttttctattt gaaaatggga
    10201 ttggcactaa taaatacgtt gtctgaagaa agtgatccgt ctgcagaaac ttcataagca
    10261 tgggataaac caacgatact ttgattgttg taaagcaagt ttgcgttatg agcagcgcta
    10321 tctttccaca taaccatagc atttactgct cctgtatctg aaaggatctc gtcaacgtaa
    10381 aggtagaaac tattgatatt ttctccttta gcaccgtagc agcctaagct ttctaagaga
    10441 tcttggaaag taccgccgcc aggtcggtaa tgactgaagc ttacttgtaa ttcttttgca
    10501 cgaatatctg cagcttgttg aacgatatca ttgaattgca ttggggcaac gccattttgt
    10561 tgacggtagc tatttaacca agcaagttct ttttgacgca catagttgtt taaatcactt
    10621 agtgagatat ttttgtattt gaaagtgatc gttcgatttt ggtcaacatt attgattaca
    10681 cgttgtccat ttttatcaac ggtaacagat gaatcgtcat ttctacacaa gaagtagttt
    10741 tgatcggtca tatttttcca agaagtagag aaactatctc cctcgtaaac cgtttgtgtt
    10801 tcagtagata aaacagcgtt tgtgtcaacg tttatatggt tcacagtgac gttgtattga
    10861 tgagtatagg caaaattgat tccttgatta cttgtaacgt tgtttaaagt cacagtgttg
    10921 ctagagcttt ctctaggatc aagaacaaaa ttagtgaaca taggtgcttc aattgtgatg
    10981 ctttgtccat aaacgacggg ataactctta ctgtaattat agagttcttg gatatttgga
    11041 tcagtatccg agactttatt tggacgagct ttcacttcaa ctagaaagac ttcatcatag
    11101 taaaaagtaa atgtggtatt tgtgttaatc gtttgtgttt gtgtttcatt cccatttact
    11161 tgatactgtg gtgcttcaaa gttagtcaag tcggttctag cttttgcagt gtaacttgta
    11221 ccagctttta ctgagacatg ttcttctttt agcagcgttc cagtttgtcg attcatatga
    11281 cggacagtta ccgcatattc tgaaggttta ggtttgtgtt gtaacccttt aaatgcagaa
    11341 tcaagactat ttttagcatt atttacttgg gtttgagaag ctttagaatc gtctagtact
    11401 tttttagcat tggataaagc agtctgaaaa ttattccaag atccttctgt aaaatctcct
    11461 ttagcagttg attttacttg gttgtacaag gcttgtaatt ccgttttgtt cacagtagga
    11521 gctggttttt tctttaatcc cttgtaagca gaatctaatg tgtttttggc actatccact
    11581 tgtttttgag tcgcttttga gtcatttaac acttttttag cattgtttaa agcggtttgg
    11641 aatgcgttcc aggtattgtc ggtatagtca ccttttttag ttcccttaac gctcgtataa
    11701 agagcttcta aagctgattt attcactttg ggtttagagg tgtcagcacc ataccaacca
    11761 ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt ccaacttgca
    11821 gtgtagtgat gcgaaccaga ggtaacacct ggaaggaaaa ggcggtagat cggtttatta
    11881 tttttttcgg cagaacgcca ggcaggccct tcataagtcc aaccgtaatt tttggtcaac
    11941 catttcactt catcccaact agctgtgtaa tggtgatcac gaaggccttt attgtataaa
    12001 cgatagacaa ggtggccacc gctagtcggt gcttcccaag cggggccttc ataattaccc
    12061 cacttaattt tcactaggtg atcacgttct tttgtgctag aggtgtaaaa atgttcatgg
    12121 ttatttggat tataaaggcg atacatgacg ttagttgaag cagcgtcagc agatccagca
    12181 agagcaactg tggcagcaaa tgttccaata gctataacag tttttgggtt taataatttc
    12241 atgttttcaa ctccctatta aatgattaaa tattttgttt acattcataa tataccacgg
    12301 tataccgagg tagtcaacgg tataccgtgg aaattttgaa ataaaaaagc cgccctatga
    12361 agagtgtaag gatggctgtt tatatgctat gaagaaatgg attcggcgta tttggttagt
    12421 ttggcagcat tatcaaatcg cattttttca atcgcacttt ttttctttat taaatccgca
    12481 attgttgatt ctgcgactga agaatgtttg ctaatttcat atgctgtctt gccagtttca
    12541 aatagtaacc attcgatttt ctctttatca gcgtacattg caatgctccc tcttgatttt
    12601 ttctttatta tagctctttt ttgtaataaa tcacaatatc aagaggaaaa gtttctatag
    12661 aaaggtggga aattatgagt atccgttccc cgtgtatcaa aatgaggttt gttgctactt
    12721 tcgttattta atatactaaa ataaaacaac gaaagaagga atagaatgaa aaagttaata
    12781 atggtattta cttcgttgat tgccatgatt gttattttag gtggatgtgg tagtgaaaaa
    12841 aataagcaag atagtcaaga taatggcgtg ttaaaaccag gaacagtgtg gaaagaagaa
    12901 gtaggcggac ttgttcataa tttaaaaatt atagatgata caacatggga atattccgaa
    12961 agtgtttggc atcctgaaca agtacaaata acagttgaga agctaaaaga ctataaagga
    13021 ttagaaagat ataaggtaat cgattcaggt ggagtagatt tattcatagg taaaacagcc
    13081 ttaatagttc cctataacaa agacggttta gaaacgttta aatttattcc aacaaacgaa
    13141 aaagaacatc gagataaaag tgaaatatta aaagaataca gtactaaatc tggatacaag
    13201 ttacaaaaga ctagcgagtg attgctagtc tttttattta tcctgaagga ggaatcgcag
    13261 tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa cgtcgcggtg
    13321 tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc acaatttgac ctattacggg
    13381 gaaaagacaa ggacttagaa ggatatatct atcaacgtga acgtgaaaag cttgttttcg
    13441 atgaaattat taaacactaa aaggagcgaa caattttatg tttgaatcaa ttacaatgaa
    13501 ttttttaaca caaccgaaag tagtagctgc ggttgatatt tttagagcat tacaaggtgc
    13561 agcaactagt ttaaaaaaca atctgatgat ttttgcggct gtagctgccg ttgcctccat
    13621 tgttcttggg gggattttct tcttctttgg ccgaagagga gctgaaaacg ggaagatgca
    13681 cgtaggttat gtattgatgg gggtaggagt tatattagct gccgcctcta ttgtggtttc
    13741 gttatttgga atgtttggac aaaccccaca gggactataa aaaataaata gaaggagtaa
    13801 aaagtacgtg gctacttttt actccttttt tatgaaagga gaatactcat gtactttacg
    13861 atttcaggca ataccaaaaa agataaagag ttaataaaaa aagcaacgaa gaaagtggga
    13921 aagcgtactg cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag
    13981 ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc
    14041 acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca
    14101 ctggattatc aaatttcaga aggtttttat cctacattga tggatttgat ccgccttgat
    14161 gtgtttaagg aaacggatga aaccatgagt gtggaagagt cttatcgaaa cgaaaaactg
    14221 ctacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgagtca agtgagtgaa
    14281 gtcgaagtaa atgatgaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa
    14341 gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt
    14401 gaggaagaga acgatgtaat gggtcaattg gaggtagatc cctttgaaga aacaatggag
    14461 gatgaagaga cggccgaagt agaagttgat ctagttgaag ctaatgaaga gccaacatgg
    14521 actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt agagaaaaac
    14581 gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag
    14641 catgaatgga tgaaagaaaa aaataaatta tttttaaatg agctttattc gaaatatcaa
    14701 ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa
    14761 ggcacacttt tagaacagtt agagtctgtt caatcaacaa attggcatga gcttgcacaa
    14821 gaagatttat tagccacttt tgaagaacgt caacaagaaa cagatcaaga aataaatgag
    14881 tatgcgacgg aacagtcatc taattgggag agaacaaaag aggaactaaa taaagaagaa
    14941 caacgagtga ttgaagaaga agtacaaaaa attaaagagc gatacgaaaa gaaacgcgac
    15001 aatgcgtatt ccttgtgtat agagaaaatt aataacttta ctaaaactaa tcaagaagct
    15061 ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtataa aagaacgaaa agaagagttc
    15121 taccgaggtt tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat
    15181 aaactttatc aaacaacgac tgaagttctt atggaaagac acgggcagat ccagcaagaa
    15241 ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa
    15301 gaacaagaag aaaaacaacg acaagaagaa aaagagcgcg aacaattaga actaaaaaaa
    15361 cgtgagcagc atcttgcaga acaaaaagtg gcgatagaaa aacaacggtt agagcaagaa
    15421 aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgacaa
    15481 gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac gccagtgaac
    15541 aaccaagcaa atttaactcc ttcaccacag cgttccttta acggatggat ggtgggatgt
    15601 attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat
    15661 caatcttcac aggcagattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa
    15721 caagcaagaa aagagtatga acaacaattg gaagaattat tgaatcgtac caatcagtca
    15781 acagcagagt caagtatgga aaatagcaaa gagagtgatc ctagtaaggc taaagaagga
    15841 tcaagcgatt caactgtaca ctcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa
    15901 taatgcgtat tagagaaaca acgagacgaa aagacaaacg attcaattta gaaaagaata
    15961 ggtatctttc tcatttctat cgctatactc cttcaggaga agagctacca gaaaaacatg
    16021 acaaacgtat ttctcaaaag attttttata gtggcttagc ttatcttcct ttaaactatg
    16081 ttgttacaag aataccagaa gcatttcatc gatttgagat tattccagga agtttacaag
    16141 gagtgaatat ttttcagtta caagaaatgc cgcagttatt ccattttgat tcaactggtt
    16201 tttggaatcc tttgacccca acaacaagtt taggcacacg tttagttgtt tctacgattt
    16261 gtgttgttcc agctgtactt atgggattga aaatggacat gtggtttcgt ccgctagcag
    16321 atgggcaaaa aggagataac cgattaaaaa cgttaaagga aatccaacaa atttatccgc
    16381 aaataccaga atcaaaggga agttttcctg gttatggcgg aataccaatt actcattata
    16441 aaaaatactg gtatattcag acggatacag taaatacttg tattgttggt acttcccgct
    16501 cagggaaagg ccagatagaa gttttagcaa ccattgacaa tttgtcgcgt gcagaaagac
    16561 aaagttcaat ggttgttaat gatccaaaaa cagaattata tgttgctagt aaagatgaac
    16621 tagaagctag agggtatgat gtgtatgcct ttaatatcac cgatccactg caatcaatga
    16681 gtgataatcc attggcactc attgtgaaat actggaagcg aggagacata gacaccgcaa
    16741 cacagttaac caatactttt acgaatacga tttattatga tgcaaatgct tcagacaata
    16801 aatactttaa cgaaaatgcg cagaaagctg tcaatgcgct tatttttgcg ttattggaac
    16861 aagcggatca aacaggggat tactccaaat taacaccaaa taatctcgtt gaactacttt
    16921 cagaaattgg cggttttaat tattatccag atcctgagaa tgaattcaag cagctcaatg
    16981 ccttagatga gttcatgaat cagttacccc ctggaaatat agcaaaaaaa caatatgggg
    17041 caacaaaaat tggatctgat aaagcgaaag ggaatatctt gtctacggca attacaggat
    17101 tgaatccatt taccctaacg aaaattgcga agatgacttc tcaatcgacc attgattaca
    17161 agtccattgg ttttccgaag tatttggatc tcaaattaca tgaaagccta ttaaatcaac
    17221 gcattgtcgt ggaattttat cataagggaa agaaatttca cgaggaacga gtcaaagttg
    17281 gctatcgtgg tttttgtgaa atcaactttg attgtcattt agagcaggga gatcatgttt
    17341 gcttaaaata caatcatcaa ggaaaacaat atgttgcttg gtatgagttt tctcaaaaag
    17401 tattgaaaga cgaagaggga aaaatcgttt atcgaaaaaa acgaggagaa actcatttgc
    17461 cagaactaga aaaagaagcc gttctaacat tagatgagaa gaaaagtaac ctattagtag
    17521 agtctattcg gatgcactat accgataaac caatggctat ttttatgtta acgcctgatt
    17581 atgatccatc taaccatatc attgttagca tctttcttag tcagttgtat aaagaattat
    17641 ctacgcaatg tgttaagact aaaggagata aatgtcaccg taggattcat tttattttag
    17701 atgaatttgg aaatatgcca ccgatggaca acatggaagg aatcatgacg gttacggcag
    17761 gtcgtaatat gttatgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg
    17821 gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga
    17881 cagatgataa taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag
    17941 agaacagtac catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag
    18001 atcgtatctt aactcctgaa agaatttcta cttttttaga aggtgaaacg attgtactcg
    18061 ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc
    18121 atggggaaac gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg
    18181 atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa
    18241 ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg
    18301 atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tcttttgaga
    18361 atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcaacg gatcgtcttc
    18421 aagaatatgt tgaaaagttg atcaaaaatg aagaaatttc tgaaatgatt aggaaacggc
    18481 tgttattgga agctgaaaag gcggtgaaag aatgatcatt ggaggaattt tctctggcat
    18541 agccgactgg tggtcaggtt tattcggaga aggggatgca gatcccaaaa ccgtaacgaa
    18601 agtcttacaa gattatggta attatctgca gtatcaagat gtattcagtt ggatttgtaa
    18661 tactttttta tggggattga tcaaagggtt atataatctc aatactttat tagaaaaaac
    18721 aatttatcaa agctttgatt taaaagactt attgaacgcc gctggtgtta atgaactatt
    18781 tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg
    18841 cttgaaattt tttatgagta aacacccacc gaaagtcaaa aatatcttgg ttaatttatt
    18901 tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag
    18961 taaaaacttt tatggagacg tcactgcagc aaataaagca gataaaacgg gttcacctgc
    19021 ttttcaaatt attcaaaata atgtgattga tgtcagtaca ttactagcga ctgacccgaa
    19081 taaagtagaa tccttaccaa cagataaaag aaatgcttta acagcgaaaa atttcaaaac
    19141 agcgaatatc aatgggatta ttacacctga tcaagctaag aaaatgtctg aatcaagtga
    19201 tattagtaca gtacaaaaag atcggttaaa ggcattacag tatcgactag agttagacga
    19261 taatgggaaa gaaatccctg ttcaaattag tgatgaaggt ttagcaaaat atgtgtatac
    19321 gtcaggctat cgtcgataca ttagtagtcc agggattatt ttggctgggg aatcttcttt
    19381 gtcggtagcc tatttattta ttttgtttac aatcgttact tgcattattg agctagtatt
    19441 taaaaaattc tatttagtaa ttgccgcgtc aacggacttt gagacaggac aacggatgaa
    19501 aagtgcgata gaagatattt ctcaatcctt cttgttattg gcgtttacga ttttagaatt
    19561 acgaatctat acattaatgc tttcaggtat cggtgatttg catgcggtag gaaagatcaa
    19621 tggattcttg tatgttgttg ctttaattgt gctaactatt gcgctattca aaggctctca
    19681 atcggttacg aagattttcg gtgttgatac gagtctgaaa aacggcagca attctcttct
    19741 tagcatgttt gccttaggaa atattgcgaa aaatgtgggt agcggagcta aaaaccttgc
    19801 aagtgctggt aagagtggtt taagccattt aaatgatatt cgtaagaatg gatttggcca
    19861 tcgaaaagat attacccatg ataacgggga agaacaatca gacgataaag ctacaactgt
    19921 acctaatagt aagaagacag tttccgaaat gttcgggaaa gcgaaaaatg gtgtttctaa
    19981 agccgcagaa ggtcttggtt acgtcaatga acgtgggtta aaaggaacag ttgaagatct
    20041 tacagaaaaa gccaaagaca ccttagaaga gaaattagac ggcacgagag ctaaagaatt
    20101 tgctgaagct gtgaagaatc catccgaagc actagaaaaa caaaaagaaa aagcttcagc
    20161 agtcaaagaa ggactaaaag atgcgtatgg tgaaggacaa gtcaatgcag cagtgaaggc
    20221 aaatacaaca agtgaaaaag aaaatcaaga aggtgcagcg attccttcag ccaaagatca
    20281 ccaagatgag ttacattcta acgaaaattc gatcggagaa aaagattcag aagaaggaca
    20341 acatggtcag ttaccaaaaa ccgatccaga acaatatcgt tataaatcta acattaattc
    20401 tgaaaacgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    20461 ggattccatc gatctgccaa atgataatgg catcgaagtt ggaactgaaa aatcaacgaa
    20521 aattggtttg cctaagacac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    20581 agagaatgat caaagctttg ttcatcaatc acctgcaggt actgttcaaa tgaaatcaag
    20641 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttagctg aaagtggtga
    20701 aacagagaat gcgtccgttc aaaatattgt gacaaaagaa gctgcaccta agccaatcaa
    20761 taaagttcat aatggataca gaccagcaaa cgtagcattt actgcaccga caaaaataaa
    20821 tgtgacaaga gaaactaaaa acaggaacaa tgaattgaaa gaacaactat tgaaggatat
    20881 caaaccataa aaggcctatt taagggcctt tttctttata aaacaagttt aaaatggaaa
    20941 ggagaaaatt gtggaggata ttttaatagc tacgtttata actgcactgg gagcattttt
    21001 aacgatattt gtggtgacta ttaaaggatt taaagatacg gatagtttac aaaaagatca
    21061 tcagtctatt ttagatggtc aaaaaacaca taccgaaagc attattgtac acctttcaga
    21121 aggattcact tatttgaaag atgctatgcc cacattcaca gtaaaattaa cggaaatatt
    21181 tcattctgtt gagcaaatat cgtcagaatt gaaagagcaa agacaacaaa aagatcgtca
    21241 acttggctca cttaatttta ggcaagaaac gatacaagaa tcattggaga atgtaaatcg
    21301 attaaacgat gaaatgcaac gagttcattt ggaaaatgaa gaattacgcg cacaaaatta
    21361 tgatttaatt caaaagaaaa gaaatctgga acaaaaaatt gttgaattaa aagaagaaaa
    21421 tcgtcaattg aattatcaat tacgacgagg aaaaaatcgt tctcgtgatt taggaaatga
    21481 attagagttg taatatttag aaaaacgctg tgagaaatca cagtgttttt tatttctaaa
    21541 aatacaaaat ataaggggta tggttatatt atggataaat acattcatat gaaaaatcct
    21601 ccaagattta gtggggaatg gaagtttttc gcctttttct ttttagatct attaacgctt
    21661 ggtggctgct ttttttgggc gaaaatagtc aataatcttg tccacttacc actctttttt
    21721 gcactcttga attatggatt atggcttgcg atagcgttag tgattgtttg gcgaccctat
    21781 aataaccctg gtcaacgcca aatttttgcg attatcgaat ctattttctt tacggataag
    21841 aaccactatc atgcgttaga tcctaaccga aattatcatg gtacgtataa ggaggatgaa
    21901 taaatgcgag tcaaaggaca aaattcagtt gataagaaat ccattttgca gtttggaaaa
    21961 ccagaagagc gctctatctt ggacttattt ccatataaat cctgtacaga agagggagtc
    22021 ttaattacta gagaagatca ttttcagcgt tattatcgtg tcgtttccac tgacgtggag
    22081 ggattaaatg aacaagagaa actagaacga atgaatcagt tgaccaccgt catgcgtacg
    22141 tatgtgccaa caattaagtt agttagtttg accacagaga cggatctaac ggaacaaatc
    22201 gtgcaaaagc ggcagttatt agataaaaat cgtatggaac agtcactagg aagaaatcta
    22261 cagcgattaa gaaaatatga aaagaaatta gtagaggaga tccaggaatt aaaacgggca
    22321 gaacaagaac gtcctgactt aagcttcttt ttcgttattg aaggaaagac aatcaaggat
    22381 ttacaaacta gaaatcgtca gttaatgcga tctagtggca ttttagggtt aaaaccactc
    22441 tacaaaaaag aattgattaa gattctttat cgaatgaaca acatgaatga cgaataaaaa
    22501 agatggagga aatgattcat gctagatgtg ataaaaacag ctttttcaac gcaaagtaat
    22561 caaaaggtga ttgatccatt aaaacaactt ggtaatcgtg aaatagagat tttaaaatca
    22621 aaaggttatg atttagattt cttaaaacaa gtgatgccac aaggtgggtt gaattttgat
    22681 gaagaatatg tgacctatgg aaatggttat tcacgatgtg tgacagttta tcattacaca
    22741 aaaaatccta ctcttttttg gttggccaat ctcatgaaca atcctcatac agtggccaca
    22801 ctagatgtta gcacagatga aaagcaaaaa gtgattgatc aaatcaaccg cgcattagat
    22861 gaattaaatg atcgatcaga taatgaacga aaagcgactg accgaaataa aagtgcggaa
    22921 gaatacctgg aactagcgga atatgcccga aaattgactt ctaatgggga aatttctaaa
    22981 caagtcaaag tgagagtaca tgtttacgga aatacgatcc aagaagtgga tgaacgggcg
    23041 caggaactga tcgaaaatct aaaagggatg gattatcgtg cggtgactta tctgcataaa
    23101 accaaacgag attggcaatc gatgtttacc atgttcgatg aacaagctga atggtttgga
    23161 aatatcaaag ggcaatcact tccatcgtta aacattggcg gaggattccc gttccaccac
    23221 caaacgctaa aagatccagg aggcacacaa tatggggaaa ccatgacagg cggcccattt
    23281 gtctggaatg ccacgtataa aaatagtgta cgtttatcta ccaatacctt gcttctagga
    23341 atgatgggag ctggtaaatc gaccgttctt aaaatgatcg cggaagccca tttagctgca
    23401 ggagacttta tttggggctt tgaaaaggga aaagacttta ttcctttctt aaaggaatat
    23461 aacggtatta tggtgcgttt agacggatct gacgggatga tcaatccttt agagatcttt
    23521 gccacacgga cgtatgatga agcatccagt ttatatgacg atggttcagt caaagatttg
    23581 aagatcaatg aagcagcgtc ctatcaaacg cacttagaca aagtagtcta tcaggtacag
    23641 ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat tcaagaccta cttgaatcgt
    23701 ttttatgaag agtatggaac agtaccacgc ggttttactt cctcaaattc gcgttcaaat
    23761 actgatacgc aagtcacagg gaaagatcca gaagcttatc cgacgttcaa agaattttta
    23821 gcctttttag gacaactgga actaccaggg gcttcccaag agaaaaagaa tcggaaagaa
    23881 gaaatggagt ctatcgtgga atccttatgt gagacttatg gcatgatttt tgatggtcat
    23941 agtacgattc gacaccttga ccaacaacag ttggtctttt ttgacattga ctctattaca
    24001 ggcctagatc gaggggtaca acaatgtcag atgtacatgg ccatgacgtt gatttggaat
    24061 caggcattga ttcaaggacg aagacaacga cgcttaatta aagaaaaaca aatggaagaa
    24121 caagacttca aacgcttctt ggcgatgatt gatgagtgcc ataatgcgat taatccagat
    24181 ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag aaatgcgtaa agtagaagca
    24241 atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat gtcttctgat
    24301 aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt tatgcgtatg
    24361 gatgaatcta ttttacgtca tatagaagga ttagttggta aatcaatgac gaactcagaa
    24421 ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa
    24481 accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga tggaggaaaa
    24541 taatggaaaa ccaaaatgaa agtttaatca aacagtatgt taagcgtcgt gcaaagcggc
    24601 gcttattttt gtgggtgttt ggtacttctg caggtttgat tactatattg atcactgtgt
    24661 ttgtgaccct ttttctaata cttgcggcag gttctattga taattctgat tcaagtagtg
    24721 gtggcgaagc ttttacagga gaatattcgg aaggcttacc catttataaa gaaatcaaag
    24781 gaagaggccc tttttctgat gagattgcgc aatatgctgt aggtgcagca gtgaagtata
    24841 aattattacc aagtgtgatc ctttcgcaat acggttatga atcagcgttt ggtacgtcgg
    24901 cgtccgcaag aaatgatctc aattattttg ggattacgtg gtttgatggg tgtctgtttc
    24961 ctaaaggaac tgcgcgtgga attggtggca ttgaaggtgg ctggtacatg aagtttccca
    25021 actcaaaagc tgccttctcc tattacggct ttatggtagc cacacaatca aattttaatg
    25081 cttgtgtcgg aaataaaagc cctggcgcaa gcttattgat cttaggacga ggagggtatg
    25141 ccgccgcagg aattactgaa gacagtgcct attataaaaa ttgtatgtct attattaata
    25201 caaataaatt gatggagtac gatgagtttg cgattaaaca ctggagagaa ggcggcggaa
    25261 ctggtggtac gattacagga agctggggca atccattccc aggaagtagc ttagataaaa
    25321 attcgttcag tggaggacaa ctttttggga aaaatccagg aggagaattc cgacctaacg
    25381 ggttccatga tggactagac tttggttcag tcgatcatcc aggaagtgag attcatgcgg
    25441 ttcatggcgg aaaagtcgtg tacgttggca atcctggtat ttcaggtttg ggtgcgtgtg
    25501 tgatcgtgat taattacgat ggcttgaata tggtgtatca agaatttgca aatagtacag
    25561 gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg tcaagtaatt gggatacgag
    25621 atacagcaca cttgcattta ggatttactc gaatggattg gcgtcaagca caaggacatg
    25681 cattcattga tgatggtaca tggatcgatc cgttaccatt tttgaatagc agcaaaaaat
    25741 agatcaggag aaaaaatatg gaaaataaaa ccttaaaaat cgtcttaaca gctgcaatta
    25801 gcgttagttt aatcggcaat attgcgttgt tttggcagct taaagaagaa caagagacaa
    25861 atacctataa aattgaagaa gtggctaaaa acgcgtctga taagctcaaa aaaaattctt
    25921 ctgaagcaca ggcgaagatt gacaagttaa atcaaaccat caatgagaaa aaccaagaaa
    25981 tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa
    26041 gaaagacggc agaagagttc ggtcaattgg ccattgataa atcattaagc agaaatgaat
    26101 taacggaaaa actaaaagat gtcgcaaccc aagaagtgat cgacaaatta agtcctgtag
    26161 acgatgatca ccaacatgat gattacggaa gttactcttt tacattaggt gacgtgcaaa
    26221 cttacgctca aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt gttgattata
    26281 caatcggcaa tcctcaattc aaagatgtca aaccacaaaa aataaaaggt ggattaactg
    26341 tcactgaaaa acaagtagat ggtcaatgga aagtcacaga ctttagttat ttcactcgtt
    26401 aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac gttttaaaaa acgaaaaagt
    26461 tatcttagat ggctggggat ctttatgatt ctcggctttt tgattcttgc tagtccgatt
    26521 tacacacata aaaaggtaga cataccagat gaagtaagca cgaacgcatt tttaaacacg
    26581 aaggaactga cgatggtttc aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt
    26641 gcgatcgacg atgaagatca gagcttatta aaagaactag ctaatcttaa ttatcgtgta
    26701 gaagtaaaga cagctaaagg ggattatcaa gcaataaaag ccaaagtgat caaggtgtct
    26761 gatgattatt ttgtagtgaa gttagcacat gtaccagaag aatatattgc tatgcgatta
    26821 actattattc ctgaaaaaat tgatccaaag gtggatatgc aagaaccgca agatctgatt
    26881 ttttatattc acgaagataa ggtcaaagat cacgtgaaag acgaagatta tgaacaacat
    26941 gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa aagcagcaca aaaacaaatt
    27001 gaatccttcc aagcaaccat tgatttgaat gaagaattag tgaagaaact aaaagatcaa
    27061 ttgccttatc aagtcgcaga agatcaggaa aatactgaaa ataagattaa tagttaccag
    27121 caagaaattg aaacatcgaa aacgcaaatg aaagatcaag aagagagcat tcaaaaactt
    27181 caagaaaaaa ttgatcgtat gacaaaagaa aatatttaga agaataggtg gagtaaaaga
    27241 aatgaagcga gcgaacttgg tcggcaaaga tactttcgct gagcagaaag tattgattaa
    27301 agcctatctc aaagttgtaa aagctaaaga aatatgtcaa gaagtgaatt atctaacaca
    27361 acaatatctg agttgaagaa tggtagaaag agtttagata ccttaaaatt cagatcattt
    27421 gaagctctat atatagctgc taaaaaatat tttacagctg ataataaata aaaaattaag
    27481 gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg gattagctat atcgtcctat
    27541 ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg aacgcttttt cttttgttct
    27601 ctgacgaaga tctggtactt aaggggttaa tgagtacgtt atcgtgtgtc atgttacttt
    27661 atttatgtat acagatcaac gaaattatgc cccctcattg gcaagaaaag aagaaagaat
    27721 ttaaggaaga aatcgaaata aaatccaatg actggattga atacacatgg cttcctctga
    27781 aaagaaatag caaacgaaag gaattttggg cattgcaatt atggccaata ggaaaacttt
    27841 ttatttatat ggcttcactt atggttactt taatcggtta tttggtggtg gtctatgggg
    27901 tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt ttcacggcag
    27961 taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc gtccaattta
    28021 tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta cttatcttag
    28081 gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg gtagagggga
    28141 taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat ttgtttgaag
    28201 taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa agatcaatcg
    28261 aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat tacgttgatt
    28321 ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc catgaagtaa
    28381 ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca atcgaccgaa
    28441 gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc attgggaaat
    28501 tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga aaagtggtga
    28561 tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat gattcttctt
    28621 tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga gcagttattc
    28681 aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat gaacattcaa
    28741 taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat tcgagagcaa
    28801 cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg ggaacaattc
    28861 aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag cataaggaaa
    28921 cgtgtaccca ctttcaaaat aaagtgggta gataggctgt gcctatcacg tttctgaaaa
    28981 aaataaatga ccccaaaatt ttcaatttat gtgtaggttt atgggtagca aaaatatgaa
    29041 tgtgggcagc aaaaagttca tttgtgggta gtaaaattca aatgtgtgta gcaatgtgtg
    29101 tagctacaca cattgctaca cacgctataa catatagtga aaaggagata tttatggaag
    29161 taaaaataag agatattgat gaaaaaacga ttcaaagaat tgaccgaaga tgtattgaat
    29221 taagtgaacg caccgggact aaatggagcc gaaatcgata cctaaaagtt ttgattgaaa
    29281 atgattttga ccatgaactt atggatcata gaaaagatca atttgatcgg ttgttagaaa
    29341 aatttgtgga tattcaaact tataatacga aaattttaga agaatatatt gcaacaaata
    29401 atcaattaat cgccttgtta attgaataat aaaaatagta ttgtgaattt ataaaaggag
    29461 gaaatcaatt ttggcaaaag caacaagaaa acatgtatat ctttacgata tgcatatcga
    29521 agtacttgat gaaataattt ctactcatga agatatatct aattatagtg aagcagtcag
    29581 acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa
    29641 attgaatgca atgggaaaag aaatttctat attaaccgaa atggtttcag agtttgcgga
    29701 tcttcatttg aaagatacag gaatactaat tggtacaaat tcaactgtct atcaagaagc
    29761 aaaagagcgt gtggaaggga aaataaaagc gaatcaaacg aaaaagtatt ctcctaagat
    29821 caagaaacca ataaatgaat ctgttcatgc cagagtagac ccgtttgatc caacaaagtt
    29881 tttaaaaaga taaaaagtag aaggggaaca tgatgaataa atcgcctgca gtcattctca
    29941 cttgtcaatt taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa
    30001 gagaaaaagc gctggaagaa aaggaacgac gcacgccgga cgaagaacag gaactgcaac
    30061 gaatcaagaa tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta
    30121 agacagataa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
    30181 tcaattttga caagttagag gaacaagatt ttaccaaata tctttcttat atgacccgtc
    30241 actatgcgtt agaacaaaag aaagaactga cgcaaaggga acaaaaagaa taccaacggt
    30301 tgaaccaagc aatgaaaaaa tatcaaacag agccacataa aaaagcttca ggaaatcttc
    30361 ctggagtttt ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaaac
    30421 aaatcttcca aagagggcaa caaaaaggct caatcattta tcaagatgtt gtctctcacg
    30481 acaattcgta tcttgaaaaa atgggcttgt acgatcccaa aacagatacc ttagatgaag
    30541 aaggattaaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
    30601 atgaaacagg ttactggatg gcaacgattc accgtaacac taagcatatc catattcatt
    30661 ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga
    30721 gttatatcgt accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt
    30781 atgtacatga gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac
    30841 gaaatttatt gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc
    30901 cagcgcgtta cgatcgacac gcaatgaact tattaaatga agtatataaa accctacctg
    30961 acaaacgttc ggaatggcat tatggggatg aaaaacgaac caaactttca ccatacacaa
    31021 gagagaaact cgatgagtta accaaatatg tgttaacaaa agaacctgaa tatcaagaat
    31081 atatacagat ttcaaaatca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga
    31141 gagaatcaaa ggatgcagaa aaaaatgctc tctttgattt gaagaagcgc acaggaaatg
    31201 ccattctaac agaactgaag aaacaggata gtcaaataaa acctttaata gaaagtatgg
    31261 atcagatgca atctatttac aatgaacgat cctttcgaac gcttaaacag ttaccaacga
    31321 atgaatacaa atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag
    31381 ttgtcacgca acggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat
    31441 accacgcaag taaagcctac gaagagcaac aacgaagaat tcaaatggaa caagaaagac
    31501 agtaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga
    31561 aggatcatta agagaagaac gaatcgaaca acttcccgat tgtttagaaa aagagcattt
    31621 gtttcagata agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt
    31681 tgagtactca gctactggtg tgtactgttg taatcatgtg aagggtgaaa aatattttat
    31741 cattcaagaa tatgagggca agttagcgcc ttattatact acttgggaaa tgaatgaaga
    31801 ggggatcaat aacttcccgt gtaaaagcat tgaagagtct atttcactca cagaatgtta
    31861 gttgttaagt gaataaaaga agtaaaagag tcctgaacgt aattgtccag gactcttttt
    31921 ttcaatgaat tgttttccgc caattcaatg agtggtgcga tttgcgacta cattatatca
    31981 aaagaaagag aagggaggaa gaagaaatgg cttattcaac gattggagtt gttgttgaaa
    32041 aaagcagaga caatctcgtt tttgtgacag aaattcaaac gggcagagct tttgtcgtca
    32101 cagacaaagc ggcaaaagcg tatcaaagtg gcgatatttt aacgttaaat ctgactacga
    32161 agacgttcgt cgatgcggca gaagattatc catt
//
LOCUS       DACEJZ010000023        31605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEJZ010000023 DACEJZ010000000
VERSION     DACEJZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.023"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(180..437)
                     /locus_tag="IW656_000991"
     CDS             complement(180..437)
                     /locus_tag="IW656_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836711.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            600..1574
                     /locus_tag="IW656_000992"
     CDS             600..1574
                     /locus_tag="IW656_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0836712.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            2304..2621
                     /locus_tag="IW656_000993"
     CDS             2304..2621
                     /locus_tag="IW656_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836713.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2825..3190)
                     /locus_tag="IW656_000994"
     CDS             complement(2825..3190)
                     /locus_tag="IW656_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836714.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            3398..3880
                     /locus_tag="IW656_000995"
     CDS             3398..3880
                     /locus_tag="IW656_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836715.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4077..4967
                     /gene="pstS"
                     /locus_tag="IW656_000996"
     CDS             4077..4967
                     /gene="pstS"
                     /locus_tag="IW656_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ0836716.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            5292..7763
                     /locus_tag="IW656_000997"
     CDS             5292..7763
                     /locus_tag="IW656_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0836717.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7866..9602)
                     /locus_tag="IW656_000998"
     CDS             complement(7866..9602)
                     /locus_tag="IW656_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0836718.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9599..10303)
                     /locus_tag="IW656_000999"
     CDS             complement(9599..10303)
                     /locus_tag="IW656_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836719.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10434..10937
                     /locus_tag="IW656_001000"
     CDS             10434..10937
                     /locus_tag="IW656_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0836720.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10897..11235
                     /locus_tag="IW656_001001"
     CDS             10897..11235
                     /locus_tag="IW656_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0836721.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11256..12674
                     /gene="ffh"
                     /locus_tag="IW656_001002"
     CDS             11256..12674
                     /gene="ffh"
                     /locus_tag="IW656_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0836722.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12786..13364
                     /locus_tag="IW656_001003"
     CDS             12786..13364
                     /locus_tag="IW656_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0836723.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13368..13889
                     /locus_tag="IW656_001004"
     CDS             13368..13889
                     /locus_tag="IW656_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0836724.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13968..14522)
                     /locus_tag="IW656_001005"
     CDS             complement(13968..14522)
                     /locus_tag="IW656_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0836725.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14775..15050
                     /gene="rpsP"
                     /locus_tag="IW656_001006"
     CDS             14775..15050
                     /gene="rpsP"
                     /locus_tag="IW656_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0836726.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15062..15313
                     /locus_tag="IW656_001007"
     CDS             15062..15313
                     /locus_tag="IW656_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0836727.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15438..16229
                     /locus_tag="IW656_001008"
     CDS             15438..16229
                     /locus_tag="IW656_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836728.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16399..16920
                     /gene="rimM"
                     /locus_tag="IW656_001009"
     CDS             16399..16920
                     /gene="rimM"
                     /locus_tag="IW656_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0836729.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16910..17671
                     /gene="trmD"
                     /locus_tag="IW656_001010"
     CDS             16910..17671
                     /gene="trmD"
                     /locus_tag="IW656_001010"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0836730.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17807..18154
                     /gene="rplS"
                     /locus_tag="IW656_001011"
     CDS             17807..18154
                     /gene="rplS"
                     /locus_tag="IW656_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0836731.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(18286..19422)
                     /locus_tag="IW656_001012"
     CDS             complement(18286..19422)
                     /locus_tag="IW656_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0836732.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(19538..20104)
                     /locus_tag="IW656_001013"
     CDS             complement(19538..20104)
                     /locus_tag="IW656_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836733.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(20240..20893)
                     /locus_tag="IW656_001014"
     CDS             complement(20240..20893)
                     /locus_tag="IW656_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836734.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(20943..21371)
                     /locus_tag="IW656_001015"
     CDS             complement(20943..21371)
                     /locus_tag="IW656_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0836735.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(21376..21750)
                     /locus_tag="IW656_001016"
     CDS             complement(21376..21750)
                     /locus_tag="IW656_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0836736.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            22054..22239
                     /locus_tag="IW656_001017"
     CDS             22054..22239
                     /locus_tag="IW656_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0836737.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            22252..22500
                     /locus_tag="IW656_001018"
     CDS             22252..22500
                     /locus_tag="IW656_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836738.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(22803..23261)
                     /locus_tag="IW656_001019"
     CDS             complement(22803..23261)
                     /locus_tag="IW656_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836739.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            23429..23746
                     /locus_tag="IW656_001020"
     CDS             23429..23746
                     /locus_tag="IW656_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836740.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            23679..24158
                     /locus_tag="IW656_001021"
     CDS             23679..24158
                     /locus_tag="IW656_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAQ0836741.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            24608..25039
                     /locus_tag="IW656_001022"
     CDS             24608..25039
                     /locus_tag="IW656_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836742.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREIEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            25036..25362
                     /locus_tag="IW656_001023"
     CDS             25036..25362
                     /locus_tag="IW656_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0836743.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            25481..26167
                     /locus_tag="IW656_001024"
     CDS             25481..26167
                     /locus_tag="IW656_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0836744.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILYGIVRLPE"
     gene            26142..27491
                     /locus_tag="IW656_001025"
     CDS             26142..27491
                     /locus_tag="IW656_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0836745.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            27482..27955
                     /locus_tag="IW656_001026"
     CDS             27482..27955
                     /locus_tag="IW656_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0836746.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            27957..28457
                     /locus_tag="IW656_001027"
     CDS             27957..28457
                     /locus_tag="IW656_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAQ0836747.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            28472..30784
                     /locus_tag="IW656_001028"
     CDS             28472..30784
                     /locus_tag="IW656_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0836748.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTGEQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            31031..31348
                     /locus_tag="IW656_001029"
     CDS             31031..31348
                     /locus_tag="IW656_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAQ0836749.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            31345..31506
                     /locus_tag="IW656_001030"
     CDS             31345..31506
                     /locus_tag="IW656_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0836750.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDVHIENL
                     RREIRRLEE"
BASE COUNT        10563 a         5397 c         6334 g         9311 t
ORIGIN      
        1 aaagagataa gactttacat tcctaaaaaa atgtaaagtc ttatctcttt tcttaatgct
       61 ataaattcat aataacatta tggacaataa atttaaaatt tattcagact gtagacaaac
      121 tcctaaattt agagtgttgt ttgcagtctg acgactcaaa aatgagtcgt ataaatcaat
      181 tatagtaata agtcattgat ttctttaaat tctttgtcaa gttctttttc atcatatttt
      241 tcatatttat cagcttcatg aagaactttt ttaatttcat gaatagcctt tcgttcaact
      301 aaccattttt ttaggttatg ttttttttct ggattttcag ataacgtatc aagttcgtct
      361 aactctttat ccagtttatt gacaacagaa acaattttgt ttacaacttt ttcttctttg
      421 ttttctaatt ttgacattta tcttcacgtc ctttttattt gataatttaa gtatagaact
      481 ttgatatagt ttatacaaat aaaaacgctt ctttgtgtat tctgtgcttt tcttttggaa
      541 tgaggaaaac tttggagtaa tatgaacgtg gacagaaaaa tatttaggag gaataaacta
      601 tgtgtacgtc tattacttat gtaacaagtg atcattattt tggaaggaat tttgattatg
      661 aaatatctta caatgaagta gtcactgtta ctccaagaaa ttataagttg aattttcgaa
      721 aggtaaatga tttggatact cattatgcaa tgattggtat tgccgctggt atagctgact
      781 accctcttta ttacgatgcg acaaatgaaa aaggattgag tatggctggg ctaaattttt
      841 ctgggtatgc tgattataaa gaaatacaag aagggaaaga caatgtatct ccttttgaat
      901 ttattccttg gattttagga caatgctcaa cagtaggaga agctaaaaaa ttgttaaaaa
      961 atatcaattt agcaaatata aattatagtg atgaacttcc tttatcccct ttacattggc
     1021 tattagctga taaagaaaaa tcaattgtca ttgaaagtat gaaagatgga cttcatatat
     1081 atgataaccc tgtgggcgtt cttaccaata atccttcatt tgactatcaa ttatttaatt
     1141 taaacaatta tcgtgtctta tcgagtgaaa ctcctaaaaa taatttttca aatcaaataa
     1201 gtttgaatgc ctatagccgc ggtatgggag ggataggctt gcctggagat ttatcctcag
     1261 tatctcgttt tgttaaagcg acttttacga agctgaattc tgtatctgga gattcagagt
     1321 cagaaagtat tagtcaattt ttccatatct taggttcagt agaacaacaa aaaggtttgt
     1381 gtgatgttgg tgatggaaaa tatgaatata caatttattc ttcttgttgc aatgttgaca
     1441 aaggaatcta ttattatcga acatatgaag acagtcaaat tactgcaatt gatatgaata
     1501 aagaagactt agatagtcat aagttaatta gttatccaat tatagaaaaa caacaaatta
     1561 aatatataaa ttagttaatg tgttgtgatt gattatttaa tatagatata atacaaaaag
     1621 gcaaagatat tttcaaaaca ttttttcttg atgtgatatc cttgtctttt ttgtaatgtt
     1681 tttaacttat tgcttttgca aaatttattt acctgttttc tggatcaacg atagtgtata
     1741 ttatgctatc cttgtttgga ttttaggacg tttaaagagg cactttttta cataacttaa
     1801 tccatagacc ttttaagcat gctgtgagat aataataaag aagagtttaa agcgcacccc
     1861 aaaccacttc cccataagtg tattacgctt taaactcttt tatatttgaa ttcactaaga
     1921 agcataccat atttttgaaa aaaagtgaga aaaaaggctt ataattagga tttagagtaa
     1981 ttaattagat gtttatttta taggactgat attatagatt acaaatagta tcgtatttcg
     2041 caatcttaaa attatcttct tactatgaat aagataataa aatttgtatt tgtccttttt
     2101 aggtaactta ttttaatcta tatacctttc tggatcaacg aaagtatact ttatatagtc
     2161 ataacgccga tggtaactat gcactgtaac gataatgctg ctaaaatatc tttttacgaa
     2221 aaacatcaca aggaaaaact taactatggt attatctaaa cataattttt tcggtattta
     2281 atatttaatt aggagggaat attttgcttt tttatagtaa ttttatctta atagtagcga
     2341 tattattact tctgaatata tggatttttg ataggagtag aaatgcaagt ataggtttta
     2401 gaactaaacg aagtctgtct tctaaaaaaa attgggtata ctcacaaact attttttatg
     2461 gaggaattgt tttaattagt ttactttctt caacacttta ttctttgaat attatagatg
     2521 tttccacctc aaattcaata tctattattg gaatcattat tgctgcaatt attacacaac
     2581 tgtttttagt gtttggagag aagaaaagaa gtaaaaaata gaaaaactag tgatagtttt
     2641 ggtgatagtg atattaaaaa tcagaaaaga tagaaaaagc aggagataga taaaatgctg
     2701 atatttcagc attttttaag agtataaaag agaaaaaaag taataaatgt atacatgata
     2761 taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca tatatggttt
     2821 gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac gacccaagga
     2881 tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat
     2941 gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg acagtagata
     3001 taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa tatccagcaa
     3061 aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga aaatcaagca
     3121 gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag
     3181 tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt ttattgtaaa
     3241 ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga ataaactctc
     3301 atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag aaaattgtat
     3361 agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag cagtaatcga
     3421 tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta catgggtaaa
     3481 tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat ttgtggtttc
     3541 taggaatttt ccagctttga ctgttttcta tttagtgata agtgtaggtc tcttattacc
     3601 aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct tattagtatt
     3661 tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt tgagcgagag
     3721 tggattgaag gattcagctt attcctccat gtatttcttg acgatcaaac catggttcta
     3781 tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag aaatcaggat
     3841 aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca tcagtaaaaa
     3901 agcatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat tatgtaaaga
     3961 atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca gcaaccatac
     4021 gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc aataaaatga
     4081 aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc ggctcaggat
     4141 caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat gaacaagtaa
     4201 aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca caagaaagct
     4261 ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc ggaactggtt
     4321 tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt gcagaagaaa
     4381 gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc gggatggcgc
     4441 caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg attgatatct
     4501 ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa atcaacgtcg
     4561 tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga ctggatggtg
     4621 ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag ctggtaagtc
     4681 aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc acacaagcac
     4741 taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc tggggaatct
     4801 ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa aaattcttag
     4861 actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc tatttaccaa
     4921 tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta gcagataaac
     4981 atgccacggt agtcgttact attaaggaaa aatcaactat gtttcccaca tatacatttt
     5041 tcctttaatt gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
     5101 tcagctgatt aaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtagct
     5161 ctttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt
     5221 ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc
     5281 gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa
     5341 gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc
     5401 aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa
     5461 caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct
     5521 tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca
     5581 tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac
     5641 taaatgtttt aaatacagca aaagtcaatg tcatacggga cgggatagaa caaacaatag
     5701 atatagaaga ccttgtaata gatgacattg ttattttaga gactggacat caaattccag
     5761 cagatgcaga ggtcgtggaa ggaaaactgc aagttaatga ggctttattg acaggcgaag
     5821 cagatgaaat aaccaaagaa ataggcgatc cattaatgtc cggcagtttc attgtttccg
     5881 gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag
     5941 tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat
     6001 tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt
     6061 acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg
     6121 gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta
     6181 aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg
     6241 taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa
     6301 aagtgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca
     6361 ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat
     6421 attttacaaa aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa
     6481 aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt
     6541 tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc
     6601 gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag
     6661 tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat
     6721 ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca
     6781 ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat
     6841 ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg
     6901 tcaagccaga acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta
     6961 tgacaggtga tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga
     7021 tggcttccgg aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact
     7081 tttcacgcat gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat
     7141 cagccagctt gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga
     7201 tctttgctct gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta
     7261 tcggacttcc atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat
     7321 tcatcatgaa tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg
     7381 gtgcgttggt gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat
     7441 ctaccatgct tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga
     7501 ataaattcag aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct
     7561 ttttgcataa gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca
     7621 ttctattttt ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt
     7681 ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat
     7741 cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc
     7801 actcgtgtat ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct
     7861 cttttttaca ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct
     7921 acagtaatcg taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg
     7981 gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt
     8041 tcctgatcct caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct
     8101 gttgcctgat agcggataat aatctcgctg tcgctttttg aatattggac agcattttca
     8161 atcagatttt tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt
     8221 ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt
     8281 tgctggaaat aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt
     8341 gacagctgga tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc
     8401 tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta
     8461 aaaccaatca gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc
     8521 ttttgtagct tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt
     8581 ccggtacctt ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct
     8641 gtaagggtga tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga
     8701 atgagttttg gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg
     8761 tttaaatgta gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc
     8821 aggaatacgc caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta
     8881 cacgcccgat acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct
     8941 tcccattgat cgggtgtttg catgattact tccgcctgat taggattttg caccattttt
     9001 tttagtacgg gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc
     9061 attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc
     9121 tcagttctcg gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg
     9181 ccactttttt tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg
     9241 cgcaaagcgc tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt
     9301 cgctgttcat tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt
     9361 aaagtaatcc gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct
     9421 gcttggaggt tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag
     9481 ctttcttgct gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca
     9541 aaaaacaaac taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc
     9601 atcgttttgg ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttggat
     9661 gttttggatc tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc
     9721 tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac
     9781 ggtcttttcg tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca
     9841 gatggtcgtc tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata
     9901 gatattcagg ctcagcttca tcttttgatt gcttacgagg ttcaatccgt ctgaatattg
     9961 ctttcatacg ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac
    10021 ctatttctag gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg
    10081 gtgtttcatt tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa
    10141 gcatcacatc taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc
    10201 ctccatctgt ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg
    10261 ttacaataga aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat
    10321 aggtttcatt ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga
    10381 aataaatggt ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg
    10441 gaatcagtgc atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac
    10501 cagaattgat ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg
    10561 gcggattacc aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt
    10621 ggaaagatca agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa
    10681 atggcgcgat caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga
    10741 aggaaaaaag cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa
    10801 aaaaaaatgg tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct
    10861 ctgatgaaga atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga
    10921 atgaacgcgc tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg
    10981 gaattatatt atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc
    11041 cgacaagcag tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa
    11101 aaactccatc ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca
    11161 tatattacag ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa
    11221 gcaatcgaag aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac
    11281 gtctccagca ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa
    11341 aagaaatgat gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag
    11401 tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt
    11461 tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt
    11521 ctgaaacagc agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc
    11581 ttcaaggggc aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg
    11641 aaaatgctcg tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat
    11701 tgaaagtatt aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc
    11761 cggtagagat cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat
    11821 tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa
    11881 tcaaagaatt gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag
    11941 atgccgtcaa cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca
    12001 ctaaacttga tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg
    12061 caccaatcaa attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag
    12121 atcgtatggc tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc
    12181 agcaagacta cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat
    12241 tcgatttcaa tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag
    12301 atctaatcaa aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag
    12361 atccaaaaga tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag
    12421 caaatcctga tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca
    12481 gtgttgttga agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc
    12541 aaatgtccaa aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca
    12601 aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga
    12661 aaaaacggaa ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg
    12721 agcttttcta tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg
    12781 cacagatgag atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg
    12841 tctggggtgt tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta
    12901 atcaagccgg attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat
    12961 atgatcgatt cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa
    13021 tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca
    13081 aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt
    13141 ctaatggaaa aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc
    13201 ctctttcaga tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca
    13261 caacaatcta tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt
    13321 tccgcaaaga agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca
    13381 tattttatgg tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat
    13441 ggctttttga cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg
    13501 atacgaccat catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag
    13561 caatttatcc agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag
    13621 atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag
    13681 gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa
    13741 tgattgatga atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat
    13801 tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat
    13861 ttatcgaatt gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg
    13921 cagaaatgtc ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat
    13981 ttgttagaaa aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca
    14041 acgatattac cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc
    14101 tgcaaggttg gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag
    14161 ccgccgatcc cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg
    14221 tgtccataag gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg
    14281 ttgtattcag gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga
    14341 gtccattcag caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca
    14401 tcgattttta caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct
    14461 acttttttgt tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc
    14521 atacgatttt tcctccttca aactatctat aataatctta caaaagataa gtaactggag
    14581 caaataaaaa cacttactga caaaagctac atagaagata gattacttat ttttaacaaa
    14641 aaagaagtct aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa
    14701 aaaaagcttt acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga
    14761 ggtggaaata aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc
    14821 cgttttaccg tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa
    14881 ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag
    14941 ttcttgactg gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa
    15001 aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga
    15061 tatgaaagat ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga
    15121 tcaagtcaag ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc
    15181 tcctgaagat attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac
    15241 aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga
    15301 taaggaaaag taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt
    15361 ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag
    15421 gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa
    15481 gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg
    15541 aaactgttac cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa
    15601 tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa
    15661 atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac
    15721 atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg
    15781 ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt
    15841 ctagtcaaga aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag
    15901 tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg
    15961 aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc
    16021 ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg
    16081 aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa
    16141 gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga
    16201 agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta
    16261 actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt
    16321 aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa
    16381 aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa
    16441 ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa
    16501 aaaggaacgg tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa
    16561 agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat
    16621 gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta
    16681 gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact
    16741 gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa
    16801 cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat
    16861 ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga
    16921 tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa
    16981 ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa
    17041 caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt
    17101 ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag
    17161 agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc
    17221 acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg
    17281 cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg
    17341 tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac
    17401 ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat atactcgccc
    17461 ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat
    17521 tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt
    17581 ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga
    17641 acaagaaaaa acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt
    17701 tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc
    17761 tgtgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat
    17821 cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac
    17881 tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg
    17941 tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc
    18001 taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga
    18061 agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg
    18121 aaaagctgct cgtatcaaag aaatccgtcg ttaatcatcg ttatcagaca gagaaagaac
    18181 ccttggtatt caagggttct ttttttatta tctgtttttt tatattaacg atttctattt
    18241 ggtacatttt tgattaataa aaaggggcaa aaaaggggca acaagttaca gtttttctat
    18301 atcttctttc aatttttctt ttactccttt tgtcacatgc aaataaattt tttcagtgat
    18361 atcactattt tcgtgaccaa cgcgatcctg aatggcatac agaggagttc ctagttccgc
    18421 taattttgaa atatgggtat gtctaaaaat atgtgaacta agttttttat caatcttcat
    18481 gtcagcttta tgacttctca aataggtgtt aattgctgtt agttggaaag gagttccttt
    18541 cgttgtttga aatagaaatt gaccatttgg atttaattct agaagctcat tgtaaatagc
    18601 tatcgctttt tttggtaaat caatttctcg cattccggca gctgtttttg tagaatcaga
    18661 ttttttcata tcagctattg aacgttctct atacatcatc gtcccattta taactactga
    18721 tgcattatta ttggtaatgt gtacatcatc tttacttaat gcgattgctt caccagacct
    18781 catcccagtc aaatatagcc actggaaaag gagagaatat cttttattgt gtgaagttgt
    18841 gaaatctact agtctattat attcatcatc ttctaaaaat ttatctttaa tttttatcgt
    18901 ttttgactct cttttataat caataactac ttcatcgata ggattttttt ctacataacc
    18961 ttttttcata gcatatgaaa aaagaagatt caatttggat ttaattacac tgacgtactt
    19021 atttgacaag tcatctttat atatcatgtc ttcaaaaata ttattcaaat ctattgtatt
    19081 aataccagaa acgatgtaag tctcaggtat cttcttcttt atagtgttta atatattatt
    19141 cgtaggataa tatgtagatt ccttaacttg tcttttgtat ataactaacc attcttcaac
    19201 aagctcatga aatgttagat caggcttatg aagtgttttt tcgtttaatt ttatatcaat
    19261 ttttttattt aactctagca atgccacttt ctgagtttct cgagatttat ttttataagt
    19321 aatgctcact ttttttcttt ttctagtttt tggatcaata tatcgctcat tatatttata
    19381 tacttttttt ccgtttttat cagttttagt ttcgatccac attgttttca tctcctattt
    19441 gttgctataa taggaataga taagtaagcc gattatagca ggtttatttt tcatcacgtc
    19501 cacaaacttt ggcgagggag gggcgtgttt ttgttagtta ttgtggtagg ttatctaaag
    19561 cgtattgtgc ttgctcattt gtaaaacctt caaatataag ttgatcgtac aattgcgaat
    19621 ctgacattga tgcccaatca tcgtaacttt ccgctttagc caaagcttgt tcgttgtaat
    19681 cgacatcaac attagccagc gcataatcga ttgcatcact tggatattct tcaaactcta
    19741 gttgttcacg caaaccttgt tcagacatac cagcccaacc tagatagctt tcagctttgc
    19801 tcaatgcatt tctgtattca cgaggaacac tttcttcttg agtaacttct tctatagtag
    19861 tttcagtagt ttcagtttct tcttcaacga ctgtagattc tgctaccgat tctgcagaag
    19921 acacttctgt cacgctttct ttagttgttt cagcgacggt atttttagca tcatcactgc
    19981 ctccttgcga acctatagca ataaacacca caacaaccgc taacaaccaa aaccataccc
    20041 gtttataaaa cggtttctta actttataca ttttcccatc ttgacccata actttttttg
    20101 ccatttaaat atccctcgct tcttgttata atatatttgt gatctcagaa atgaggtatg
    20161 agtccgtgtt gcagcacggg cttttttctt tataactttt ttagagatta taggcaaaat
    20221 agtagggcat aaaaatgtat tattgaattc cgtatttaga aaatcctaat tgaacttcac
    20281 cggaagtctt ttgctgtgtt gtacgcaatg cttcttcttc agacattcca ttctgtactt
    20341 tccatgcaac aggcgacatc ccgtatttgt taacaaaatc agtaagtgat aaagtgtcag
    20401 cgtcttgctg agcgcttgtt tgttggtctt ctggattctg ttgagatgct gcttgttgtt
    20461 ctttctgatc ttgactgata atattgccag catcatctgt agtcaatcca ttttcataaa
    20521 gggccacgcc gaaagcttcc cactctttgt tggaccaatt tgcacgatca gctggagttg
    20581 actgtaaagt gcgttgtttc atctgttcat atgtttcttc ttgaggtgcg gtttggattg
    20641 tactctgact ggagcttata actgttgggc taggttccgc cgtagcttgg ttggagcttg
    20701 taactgttga actagaatct gttttagatg tagatttgct agtagatgaa ctggtttcag
    20761 ttgtttcttt tgttttactt atttttgttt cttggttaga agtggtatct gttgattcag
    20821 cttttttatt atttgaacaa gctgaaagta gcagagcagt acttaacaac aacataacgc
    20881 taactttttt cattttataa ttcctctttc tcgttgtaat atgtgtgcta acacgggctt
    20941 ttttatataa gaaaacgata agcgctttct ggaagcccgt aaagattctt taattcctcg
    21001 atttttttag gatattgatc attatcttct ttataaagag aaacaatgag attagcagca
    21061 aagcaattag cttcgctttc agatttgctt ctagatgttc ttgttgatac ataatagctg
    21121 gataagccac gatgaaaaat agcgtgacct aattcgtgag cgcaaatgta gaatctttcc
    21181 tcagagtctc gcagttcatc atttaaaaag attatcgcac gatctctaat ttcttgaaac
    21241 tgccccttgg gattctcgat aaaaggaacg tattgaattt taatgcccat cttttcacaa
    21301 atataaaaag gattagcgga ctggtatttc cgcttcaact cctcgactaa attaatcgta
    21361 tccatctcca taagctcaca tctttttgcc tttttctttg tcttctttca caatatccca
    21421 gaaagtcgct atcaggatat cttttacgcg ctgtatttgt tcgggtgtca atgtttcccc
    21481 accataagac atattaacat ttgagtctag tagtttatca agttcaacca cttcctcttt
    21541 tgtagcccac ttgggaacat tattatttcc caataaataa tcagttgtga caccaaaata
    21601 atcagcaact ttctttaagt tctcagattt tggcgaggct ttatcccatc ttcttatttg
    21661 tccattagag atgcccacct gtctttctac ttctgctata gtcacatgct tttcgtctgc
    21721 taattcttta atcttagtaa ctaaactcat tattatcaac ctttcaaagc tgaaaagaaa
    21781 ataaatagct taaaagttat atttttggtt gacaattagc ttttaagcta gtatatttaa
    21841 ttcgtaagct aaattgttag ctaaataaga gcaacaaaaa actctactaa tttaaaacat
    21901 tctctcggtc gccaaactta gaaatgttat tttagaggct ttttataagt cttatttaac
    21961 tatgtattca taatagctta aaagctaata ggtgtcaacg atttagctaa ttttttagct
    22021 tacaaattat ttgtttagaa aggagctatt tttatgtctg agaatttaga cttaaaaatt
    22081 cgagcggaga tgagaaaaag aagaatgact ttcaaagaac tagctgcgct tatcggtatt
    22141 tcaggagctt atttatcaga tattctaaac ggcaatcgtg atggaaagaa agcacaacag
    22201 catatcgaaa cagtgaaaaa aatattggac atccgatagg gggggtaggc gatgacaaaa
    22261 ctaaaaaaac aagattttgt aaaaaaatat aattattctc catctactta tcaacgtcga
    22321 atgtcggaac taaaaaatac agcaattttc tcagcggcgt atgaacgggt cacaggacaa
    22381 gaagtttgga tcaatacaga attatacgat aaatttttgt ctttcaaatc ctataacagg
    22441 ttacgcacaa gaaaggtaac acctaaagaa tttatcgaga agcatttagt tgatttataa
    22501 aaaacagaac attttgagag gtgaaggtta atgggtaaat tcaacagagc attagtattc
    22561 agcgcaccgc taatcgtcta tgctttaggg ctttggggaa gtagacaagc gttgatagga
    22621 acgattgtgt acatggtttg gatttttata gggctggatg aagctgagta caaaacaaaa
    22681 aagccagtcg ggagggactg actaatgagt aaggacgata ttgtatttct gttaattatg
    22741 ttttgtggga ttggatcaat aataattaat ttgtatttga ttattaaaga aaaatttttc
    22801 aattaaaatg agtcttttag ctttaactta aatttttttt gataattagc tgttcttatg
    22861 accattacaa gtttttcacc aggtaatata tctttcatat ctaaaacttc tgaaaaagcg
    22921 actgagtagc caggagagat tgcttgtgcc aacggtatag gagataaaag gctactttta
    22981 tgaatgcttc ccatatcaaa accattggcg tgataatgtg aacaattaat tttcttaccg
    23041 ttgtaaagat acgtacattc gataattgag tttggcattt ttgatgggtt tgcaattacg
    23101 ccagtaagaa tcattcgatt acttctatca tgcttaacta aaaaatagga gagcgtaatt
    23161 tttcttttat ctttaaataa gttatagagg gataaaaaaa atcctgaaat agaaatgatt
    23221 aaagtaatag cgacttggta atcgctcaaa atttttatca agtaattcac caccttttta
    23281 ctcaaattat atcaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata
    23341 aaaaaagaca cgaacgccgg caagcatgat cgtgtcaggg aaataaacta tctttctata
    23401 ttttaccaca gaaaggaatg aaccgtaaat gctaattgca acggatacac tagaccgcat
    23461 ctttttaaaa gacgaataca aactgcgcaa tatagatgcg tcaggaattt tagtatttga
    23521 tctttatgac aatggaaaaa ttggtatcta tcaagcaagt gatattgaag aaacaaacct
    23581 tgctttcgaa caaattgatg attctgtgga attggattta gatgaggcaa tcctagcttt
    23641 tgaacaaatt gcaaaattat taaaggaggc acaaaagaat ggcaactctt taccaactca
    23701 gcgagtcata tatcaaagtc ctggaactgg cagaacaatt ggatgaagaa attcttcgcg
    23761 atactctcga ttcgattaat gaagcgatcg agtataaggc agaaaactta gcaaaaatag
    23821 ttaaagaagt agaagggaaa gctgagttaa tcgatagtga aattaaacgt ctgcaggaac
    23881 gtaagacatc gcttttgaac aatgctaaga gtatcaagca ctatttacaa gaggaaatgg
    23941 aaaagactgg taaaacgaag attaaaggtg aattattcaa cattggaatt caaaataatc
    24001 cagtatcggt caatgtaatc aatgagaact taattccaaa aggattcttt acccctgtgc
    24061 ctcccaaatt ggataaaaag caattgaagg aggaactgaa gcacggagat attcctggtg
    24121 ctgaactcgt ccaaacaaaa ggtttgagga taagatgatg aaggaggaga aataatggaa
    24181 cacgaaaacg taaaagacgc tttgaaagct gctatcgaaa tcgcggaagc taaaggaata
    24241 aaggttgacg gcaaaccagc aactgtccat gacattcaaa atttgacgaa agaacatctt
    24301 tattttatcg ccgacttgct tggtttgtcg gaattgtatt tagacaaata accccacacg
    24361 gaaggagtgg ctagggtggt tgtattacaa gtaatagtct gcgtaacatt catttggtcg
    24421 tgcataccgg tttgtatata tatcatgaag aagcttcgcc ctgagtctta caaattatac
    24481 gcaatttaca gcataacagt agctcttgca tttacattgt ttgcaatttc taggcttccg
    24541 ttttaatcgt gggaatgaat ttttaatgaa ctaaattatc tataaagcaa aaaaaggaga
    24601 aacaacattg aaaaacaaac tagcaaaaac aacagcaatc atcggactag cactaggtag
    24661 cggcgttatc ggctacgcag caagcaatgc gtttcaagat ttggacacga tcaaagcaaa
    24721 tttcaacaca gtcctacaat acggtcaaac gaaatcgcaa cgtgtgtcag aacttgaatc
    24781 acagctatcc aacaacactc gcacacagga gcagctgaaa gccgaaattg agcaaatcaa
    24841 atcggacaag cagaaggaaa ttgaagccaa gcaacgagaa atcgaacaaa agcaacagga
    24901 gatcgctaca aagcaacagg aagccgatag cttgcgccaa caactatcca cagtgcaaaa
    24961 cgacaaggag cagttagaac agcgcgtgag cgagttacgg cagtatacgg atcaaaaggt
    25021 aggggagttg gggaaatgaa agagttgact atttatgacc caaatatttc atgggctaaa
    25081 cacacaatta aagtttcttt tatgatttgg ggttacaaag gatatgttac ttacaaagtg
    25141 ggcggtaaca ctaagggttt atcattaata gctattgatt cagatgatct atatgatgct
    25201 aatttcgagg ataacccagt taatttccgt gaccttgatg aagattggtt ctctatggaa
    25261 cttactaacg ataaaggcga cagtacactt gtagaagatg aattcgacag actaggtgat
    25321 tatattgtcg gtgttgaaat tattgcgcac gaaccagagt aggagagtga atacaaaatg
    25381 attaaaagaa tcactatatc agttgaagaa gatagtggat caacaatgca gcataccgac
    25441 aatcgaaaaa cttttttaac caatagaaag gagaattttg atgcagatta agaaggcgac
    25501 ggatatcgaa caaaccaagg gaacatattt gatctacggt gctccaggaa aaggtaaaac
    25561 ttcaacggct aaatattttc cagggaaaac actgatcctt gatattgatc ggacttcaaa
    25621 agtccttaga ggtgagaaaa atgtcgatat cgtatatatc gacaacgaag atacctggaa
    25681 cgactggggc aacacgttgg ctgatttaac taccaattat gtaggtgttt atgacaacgt
    25741 ggttgtcgat aatgtcagcg aattggaacg gtgtattctg tccagtttag gcgcagaagg
    25801 aaaaaacaac ggcgttcctt cgcaaggcga ttatcaatat atgcaattta gaatggtgaa
    25861 cagcctacgc tatatgaaaa atttagacag caaccttatc tggaccgcat gggaagaaat
    25921 cgatttgtgg acggattcgg atggatcttc ttacaacatc gctttgccgc agatcaatcg
    25981 aaagattcga aacaatattt tgggattatg tgacgttgtt ggtcgattga tggtaaagga
    26041 agacgatgaa cgcggattta ttttacaagc cacagattca acctatgcca aaaatcaatt
    26101 ggataataga tctggctgta agcaaagtga gctgatttta tatggaattg tacgactacc
    26161 agaatgattt agtcgatcgt gcaagacaag cctatgttga tggttacaag gcaccatgta
    26221 tcgtatcacc gtgcggttct ggaaaatcag tcatggttgc agagatcgct aaacgaacca
    26281 cacgaaaagg caacagagtg ttgttcttgg tccatcgtcg tgaattgatt gatcagatca
    26341 aagatacgtt tcaaaagatg ggcgtagata atcagcttgt aaatttcggc atggttcaaa
    26401 cagtcgtacg acatcttaaa accatcaaga aaccacaact gatcatcact gacgaaaacc
    26461 atcacgggtt agcagcgtcc tacagaaaaa tctatgagta ttattcggac gttccgcgat
    26521 taggttttac cgcgacaccc atccggttaa acggtagtgg gttgggggat gtgaacgact
    26581 tgctaattga aggagtatcg gctaaatggt tgattgagaa tcatagatta gctccttatg
    26641 aatattatgc acctaaattg atcgatacag ccgaattaaa gaaagcttcg actggagatt
    26701 ttacaaagaa atcaatggat aaggcagtga agaatactat ttatggcgac gtgttgaaac
    26761 attataaaac gttagcagaa ggtgaacagg caatagcata ctgtcacagt atcgatgcaa
    26821 gtaaacacac tgctgaaata ttcaacgatg ctggttataa agcaacacat atcgatgcaa
    26881 aaactccaaa agatgaacgt gcagatatta tcgaagcgtt cagaaatcat gaaattaaaa
    26941 tcctatgcaa tgttgatttg atcggagaag ggtttgatgt tcctgattgt tcgactgtaa
    27001 ttatgttgag acctactcaa tcgttatcat tgtacattca gcaatcgatg agggggatgc
    27061 gttaccgacc agataaaacg tcaatcatca ttgaccatgt gggtaatgtg aaccgttttg
    27121 gattgcctga tatggatcgt gaatggagtt tagatacaaa aaagaaaaca aaaactgata
    27181 gtgatttatc tatcgttcaa tgcgcgtttt gttttggtgc ttacgaacgg ccaaaaggcg
    27241 ataatatttg tccgtattgc ggagagttgc agccgattga agaacgaaag tcagagttag
    27301 aaatcgatga atcggctgaa ttaatgaaag tcggagaaac gaaaattact ttgaattttg
    27361 agaacaataa atattacaac atgacggaag atgaagcaga aagtatagag gacttatatg
    27421 ccatagcaag agctaaagga tttaaacctg gttgggctta tatggctgct aagaggaagg
    27481 ggtggttgta ggaatgggcg gtatttttgg taaaaaagca gaagatgttc aaggttcaat
    27541 aataaatggc gtaacaataa taggtgatac tggaaaaaga gatagttcag gcaatcaaat
    27601 tgtaatcgct agaaataaaa ataacgaagt ttttgaatct tctctatcta atataaaaag
    27661 aggtgttgtt aacgggacag gcgaagagag tggctacgtt aatttaaaaa aagctaatga
    27721 aaaataccat gttgaaggaa cttacgttaa ggctttgtct gtcggtggaa caaaaggcgt
    27781 taattgggat aaacgaagaa ataaatggaa agcacgatta tatttcaaag gagaatatgt
    27841 cttacaaaag gatttcaaca ccaaagaaga agcaatccaa gcacgacttg cagcagaaga
    27901 aaaatatttc aaacctatct tagaaaaata tgaaaataaa aaggagaatg attaacatga
    27961 caggatttaa cttagacttt tcaaaagcgc aacaagggaa cgaaatcaaa gacggtacgt
    28021 acgaagtggt cgtaaacaaa gctgtagaaa atgcaactaa atcaggcgca gaatttatcg
    28081 atatcgattt gatcgttcgt aacgatgtgg atcaaccgtt ccaaaataaa cacattttcg
    28141 caaaaatctg gaaagccaaa gctaccggca aatataacga aggtatgatc atggcaattg
    28201 cacaagcgct acagttggaa gacggaaaat catataacgg gtttgatgaa ttgctggcgg
    28261 actttgtttt gaaaaccgcc tcagtacgcg tgaaaactga agagtcaaat ggatataaaa
    28321 acgtcaatgt gaagtcttgg gataaaacga acacacgggg cgtaatgaat caccaattca
    28381 aaaatggtga tgaaccatcc ttcggaccgg aacgttcaag agcgacaacg gttagaaatg
    28441 acaatcttcc attttaattg aggtgtaatc aatgtatgaa aaaattccaa aagagcttaa
    28501 aaatctcaaa caatggtgtg tttataagct agtttgggat gaaaagcggc agaagcacac
    28561 gaagatccct tataacgcca acaacgggca taaagccaaa tccaatgacg agagtacctg
    28621 gtctgatttt cagaccgctc tcgctgctat tgatcactac ggaatgagtg gactggggtt
    28681 ctttttcaaa ccaccttatt tcggcattga tatcgataat gctgaaggtg aagttgagcg
    28741 atttaaatca ggtgatatag aagaaaatat tatctatgaa tttatcgaaa gcatgagatc
    28801 ctatgctgag tattctcaat caggtactgg catccacatt attgctcgcg gagaattgcc
    28861 cggcggaaga cgcaggaaag gtgacgtaga aatgtaccag aacggccgat tcttcgttat
    28921 gactggaaat gccgcatcaa aatatttaga aataactgag ccgaatccaa aggatatcaa
    28981 acgcctatat gatcgctacg ttggagatga aaaaatcatc caattcaaag aagaaaatcc
    29041 gctgatgaat accgttgatt taccggttga agaaattatc aaacgtgctg aatcgtcttc
    29101 ccaaggggca agattcaaag tctttatgaa cggcggttgg gaagcttttt attcctcgca
    29161 atccgaagcg gatatggcgt tcgccaacga cttggctttt tggaccggta gagactttga
    29221 aaaaatggat gagattttca gagcgtctgc aatgatccgt agcaaatatg accaaaaaag
    29281 gggatcggca acttacggag aatctctact aaataaggca attgctgata caaattccgt
    29341 ctacaacccc aaacggaaat ctgattttaa gatttttatc aaagaccagg aacagccaaa
    29401 agaggaaaaa tactattcgt acgacgacac cggcaacgcg gatcggttta ctgatatcta
    29461 cggaacgtta gtcaaatatt cgtacattga taaatcttgg tattactacg atgggaaagt
    29521 ttggctacag gacaataccg gcgaagttcg gaaaatgatc gatacgacag tagacatcat
    29581 gggaaaagaa ccgttaacga tccctgaagg cgcagacgat gaaaccaaag aagctttgat
    29641 gaaagcgaaa gaaaaacacg tcaagcgttc tcgcagcaat gccggaaaaa acgctatgat
    29701 ggacgaattg aaacatcgac tatccgtatt gccggaagag ttcgacaaag ataaaacgct
    29761 gttcaacaca caaagcggct atctgtcttt gcatgacggt cttctacacg agcatgagat
    29821 agacaaaatg tttacacgtg tttcaaatgt cgagtacaca gaatcggtcg attgtcctat
    29881 gtgggaagaa tttattaatc agatttttga caatgatcaa gaattaatca gatatatcca
    29941 gaaatgtgtc ggatattccc tgaccggatc taccggagag caatgtatgt tcatcctcta
    30001 cggtcatggg tcaaatggaa agtcggtatt tttggaaatt atttctgaat tgatgggaaa
    30061 ctatgcgatg actatgcagg cacaaacgat catggtcaag caaagccaat catcagcaaa
    30121 cagtgatatt gcacgattga aaggtgcgcg gttggtcact tcgtcagaac cgaatgaagg
    30181 cgtccgactg gacgaaggtc tggtcaaaca attgactggt ggtgataaag tcacggctcg
    30241 gcacctttat ggaaaagaat tcgagttcga acctgaattc aagttgtggc tggcaacgaa
    30301 ccacaagccc atcatccgtg ggaccgatga cggcatctgg cgacggttaa atttaattcc
    30361 attcactgtc caaatcccgg atcacaaaaa ggataaaaat ctgaaattta aattgcagac
    30421 ggagttgcaa gggatcctta aatgggcaat cgatggttgc ctgttgtggc agagagaggg
    30481 cttggagaaa ccaaagtcgg ttgttgcagc gagtcaggat tatcgaaacg aaatggatca
    30541 aataggtaca tttatcgaaa cgtgttgcga aacaggtcca ggactaaaaa tatcaggcgg
    30601 agaattatac aaagtttatc gtgaatgggc atcagataat ggcgagcata ctttcactaa
    30661 cacgaagttt ggcagagaga tttcaaagaa atatagcaaa gaaaaagctg gtggatttat
    30721 ggtttataaa ggaatcacat taaaaccaag aaaatacgat aacgttagag aactttttaa
    30781 ataaatctgg agggttctgg agggttgaga acgaaaccct ccaaccctta gaaacgttga
    30841 tatatcagta tttatattat atttttcttt ctttctggag ggttttaaaa aaataataaa
    30901 gtattaaagt aaaaaataaa aagtaaatta tataaaaaag aataggtttt tgggtctgac
    30961 cctccaaact ctccaccaaa atcttcctaa atgctttgat accaacgatt aaacagtctg
    31021 gagggttaca ttgaaatcag aacatcaaat acaagacgag atcagaattg ccttatcaaa
    31081 acatggttgc acgatttttc gatccaatgc gggacaagtg acaacgatcg aagggcggca
    31141 attttacggg atgccaaaag ggttcccaga tctatgtgga catcgtaatt ctgatgggaa
    31201 agcaatttat atagaagtaa aaaatgaacg cggtaagttg cgtgaagatc aaaaaaggtt
    31261 tgctgaattt ttaaacggtc aacctgtact atacggtgtt gcacgatcag tggaagaagc
    31321 attgaaaatt gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
    31381 agccttcagc gtgagattga cgatctggaa cggacaaagg cagtgatggt caatgaaacg
    31441 gcgaggaagg caatcgatgt gcacatagag aatttaagaa gggaaatccg tagattggag
    31501 gaatgagcgt ggataaggaa gcggcttggc gaaaattaat gttgctgatt caagatgaga
    31561 actggcaaga agatgaagca gtggttgctg aagttcagcg tctag
//
LOCUS       DACEJZ010000024        30948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEJZ010000024 DACEJZ010000000
VERSION     DACEJZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.024"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            163..1104
                     /locus_tag="IW656_001031"
     CDS             163..1104
                     /locus_tag="IW656_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0836751.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="IW656_001032"
     CDS             1173..1904
                     /locus_tag="IW656_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0836752.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="IW656_001033"
     CDS             2048..4228
                     /locus_tag="IW656_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0836753.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="IW656_001034"
     CDS             4248..4730
                     /locus_tag="IW656_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0836754.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="IW656_001035"
     CDS             4746..5507
                     /locus_tag="IW656_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0836755.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="IW656_001036"
     CDS             5638..6972
                     /locus_tag="IW656_001036"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0836756.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="IW656_001037"
     CDS             7279..7764
                     /locus_tag="IW656_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0836757.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7757..8440
                     /locus_tag="IW656_001038"
     CDS             7757..8440
                     /locus_tag="IW656_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0836758.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8566..11211
                     /gene="polA"
                     /locus_tag="IW656_001039"
     CDS             8566..11211
                     /gene="polA"
                     /locus_tag="IW656_001039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0836759.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11256..12092
                     /gene="mutM"
                     /locus_tag="IW656_001040"
     CDS             11256..12092
                     /gene="mutM"
                     /locus_tag="IW656_001040"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0836760.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12056..12685
                     /locus_tag="IW656_001041"
     CDS             12056..12685
                     /locus_tag="IW656_001041"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0836761.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12737..13069
                     /locus_tag="IW656_001042"
     CDS             12737..13069
                     /locus_tag="IW656_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0836762.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13381..13872
                     /gene="nrdR"
                     /locus_tag="IW656_001043"
     CDS             13381..13872
                     /gene="nrdR"
                     /locus_tag="IW656_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0836763.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13895..15268
                     /locus_tag="IW656_001044"
     CDS             13895..15268
                     /locus_tag="IW656_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0836764.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15268..16194
                     /gene="dnaI"
                     /locus_tag="IW656_001045"
     CDS             15268..16194
                     /gene="dnaI"
                     /locus_tag="IW656_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0836765.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16323..16763
                     /locus_tag="IW656_001046"
     CDS             16323..16763
                     /locus_tag="IW656_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0836766.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16907..17608
                     /locus_tag="IW656_001047"
     CDS             16907..17608
                     /locus_tag="IW656_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0836767.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17605..18726
                     /locus_tag="IW656_001048"
     CDS             17605..18726
                     /locus_tag="IW656_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0836768.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18741..19973
                     /gene="pepT"
                     /locus_tag="IW656_001049"
     CDS             18741..19973
                     /gene="pepT"
                     /locus_tag="IW656_001049"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0836769.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20105..21013
                     /locus_tag="IW656_001050"
     CDS             20105..21013
                     /locus_tag="IW656_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0836770.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21048..21320
                     /locus_tag="IW656_001051"
     CDS             21048..21320
                     /locus_tag="IW656_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0836771.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21331..22377
                     /locus_tag="IW656_001052"
     CDS             21331..22377
                     /locus_tag="IW656_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0836772.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22628..25237
                     /gene="clpB"
                     /locus_tag="IW656_001053"
     CDS             22628..25237
                     /gene="clpB"
                     /locus_tag="IW656_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0836773.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25388..26059)
                     /locus_tag="IW656_001054"
     CDS             complement(25388..26059)
                     /locus_tag="IW656_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836774.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26052..26546)
                     /locus_tag="IW656_001055"
     CDS             complement(26052..26546)
                     /locus_tag="IW656_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0836775.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26566..27786)
                     /locus_tag="IW656_001056"
     CDS             complement(26566..27786)
                     /locus_tag="IW656_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0836776.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            27933..29768
                     /gene="lepA"
                     /locus_tag="IW656_001057"
     CDS             27933..29768
                     /gene="lepA"
                     /locus_tag="IW656_001057"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0836777.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(29862..30944)
                     /locus_tag="IW656_001058"
     CDS             complement(29862..30944)
                     /locus_tag="IW656_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0836778.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10303 a         5542 c         6629 g         8474 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     8521 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     8581 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     8641 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     8701 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     8761 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     8821 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     8881 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     8941 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     9001 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     9061 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     9121 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     9181 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     9241 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     9301 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     9361 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     9421 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     9481 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     9541 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     9601 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     9661 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     9721 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     9781 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     9841 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     9901 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     9961 ttgagccaag aactggcaga taaaaaccaa gaagatcttt tccggaaaat ggaactgcca
    10021 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
    10081 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
    10141 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
    10201 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
    10261 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
    10321 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
    10381 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
    10441 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
    10501 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
    10561 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
    10621 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
    10681 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
    10741 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
    10801 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
    10861 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
    10921 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
    10981 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
    11041 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
    11101 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
    11161 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
    11221 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
    11281 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
    11341 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
    11401 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
    11461 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
    11521 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
    11581 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
    11641 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
    11701 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
    11761 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
    11821 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
    11881 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
    11941 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
    12001 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
    12061 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
    12121 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
    12181 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
    12241 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
    12301 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
    12361 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
    12421 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
    12481 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
    12541 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
    12601 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
    12661 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
    12721 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
    12781 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
    12841 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
    12901 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
    12961 acctgatcgt agtaaaacac cgctcccaga aagtgaccac atttccgatg agcaaaaaat
    13021 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
    13081 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
    13141 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
    13201 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
    13261 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
    13321 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
    13381 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
    13441 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
    13501 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
    13561 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
    13621 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
    13681 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
    13741 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
    13801 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
    13861 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
    13921 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
    13981 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
    14041 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
    14101 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
    14161 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
    14221 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
    14281 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
    14341 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
    14401 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
    14461 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
    14521 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
    14581 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
    14641 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
    14701 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
    14761 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
    14821 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
    14881 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
    14941 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
    15001 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
    15061 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
    15121 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
    15181 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
    15241 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
    15301 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
    15361 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
    15421 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
    15481 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
    15541 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
    15601 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
    15661 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
    15721 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
    15781 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
    15841 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
    15901 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
    15961 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
    16021 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
    16081 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
    16141 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
    16201 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
    16261 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
    16321 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
    16381 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
    16441 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatta
    16501 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
    16561 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
    16621 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
    16681 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
    16741 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
    16801 tatagaacga aaaggtatat tttttaagga gatatgggaa tttttttatt cctagtctcc
    16861 ttattttatt tggaaaatgc tataattatc tagaaggagt gaagctatga accatttaga
    16921 tttatctcag cgcttagccg cagtgggtga ctttgtccct gaatccgctc gtttagcaga
    16981 cattggatca gatcacgctt acttacccgt tgcattgata ttaagaggaa aaatcgaata
    17041 tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc aagtcaaaaa
    17101 agatggatta gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg ctatccagcc
    17161 acaagatggt attacagctg tcacgattgc gggtatggga ggttcactga tccgagatat
    17221 cctagaagct ggcaagttga atcaacgctt aagcggacaa gaacgtttga tccttcagcc
    17281 aaacatcgga gagcgtacat tacgatcttg gctgcaagaa aatcagtata aaatccaatc
    17341 agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc attgtagccg aaaaagaaca
    17401 aaaaccagtt acttacaatg aaatggagct gatgttcggg ccatttctgc ttgaagagaa
    17461 aagtgagatc ttcaaatcaa aatggacaag agagcttcaa caaagaaaag ctatccgtga
    17521 acagctcaaa caagcagcta atcaagaggc acgtcaaaaa caaatcgagc aggaaatcca
    17581 aatgattgag gaggttttaa agccatgaca atcagcggac gcactttcat ccaaaagttt
    17641 gaaagctatt gtcctcagtg gctagctgaa gaaggagacc cagtcggact gcatatcgga
    17701 acattggata agccaatcca acgagtaatg atgacattag acgttcgtcc agaagtggtc
    17761 gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc aatctttcgt
    17821 cctgtaaaac ggctggttac tgatcagcca caagaaaaaa tgtatgcaga tttgttgaaa
    17881 catgatattg ccgtctatgc agcacacacg aacatggata ttatttggga tggactgaat
    17941 gactggtttt gtgaacttct cggtattgaa gtggagagtt atttagtaaa gactcatgag
    18001 attcattata aaaaactggc cgtttatgta cctgtggatc atgcacagaa aatgcgtgaa
    18061 gtattagctg cagctggtgc tggtacacaa ggtgattata ccggtacaag ttttacctcg
    18121 attggacacg gtcgatttac tccggaagct ggtgcacaac cagcgattgg gaaagtaggg
    18181 aagacagaac aagttcaaga ggcaaaagtt gaggttattt tgccggaaac tatcgaaaaa
    18241 caagtgattc aagccatgcg gtcagctcat ccttatgagg aacctgctta tgatcttttc
    18301 gctatagatg aaccagtcga aatgtttggg ttaggacgag taggtgaatt gccgcaagaa
    18361 atttctatag aggcatttgt agaacaagta aaagaagcgt tccaactaga tggactgaga
    18421 atagtccagc caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg cggtggaagc
    18481 ggtgaaaaat tttatccgca agcaatcgcc caaagagcag atgtctatat cacgggagat
    18541 atctattatc atacggctca tgatatgcaa agcgctgggt tgattgcaat tgatcccggt
    18601 cattatattg aatcgttatg caagcaacga tttatcgaga aatttgaatc ttggaaacaa
    18661 gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa atacaaaccc atttcaattt
    18721 aattaaaagg agagaatact atgtacgaaa atcttttgcc acgtttttta cgatatgtca
    18781 aaacagaaac acgctccgat gcaacaagca aaacgactcc atcgactcag acacaagttg
    18841 cttttgcgca agaattgaaa aaagaattag aagagttagg cttagctgat gttttctata
    18901 atgaagataa cggattcgtt atgggaacat taccaagcaa tgttgatcat gaagtgcgtt
    18961 ccattggatt tatttcccac atggatacag cagattttaa tgcagtagga gtcaatccac
    19021 aaatcattga aaactatgat ggcgagtcta cgatcgtact agatgcggaa gaacgctata
    19081 cattgaatac aaaagatttt ccaaatttga aaaattatga aggccacaca ttgatcacaa
    19141 cagatggcag tacattactg ggagcagatg acaaatcagg gattgcggaa atcatgactg
    19201 caatggagat cttgatcaat aatccttcta tccctcacgg taccatcaaa gtggcttttg
    19261 gtcctgatga agaaatcggt gtcggagcgg ataaattcga tgttgagcaa ttcgatgtag
    19321 attttgctta tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa acgtttagtg
    19381 ctgctcaagc aaatattacg atccaaggga aaaatgtaca tccagggacg gcaaaagata
    19441 caatgatcaa tgctctacag ttagcaatcg attttcataa tcagcttcca gccgatgaag
    19501 ttccagaaaa aacggaagga tacgaaggtt tcttccattt gatggcattg gatgggaatc
    19561 ctgaagaagc ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa ttcgaagcac
    19621 gcaaagcaca aatcacacat atccaagaag agttgaatca gcgatttgat caagaacgaa
    19681 tcaaagtaga tatgtatgat caatattaca acatgaaaga aatcattgaa aaagatatga
    19741 gcatcgtgga attagcgaaa caagcaatga tcgagcttgg tatctctcca gtgatcgaac
    19801 cagttcgtgg aggaacagat ggttcaaaaa tctcttacat gggtattccg acgccaaatc
    19861 tatttgccgg aggagaaaat atgcacggac gattcgaatt tgtttcttta caagtaatgg
    19921 aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa cacaaagagt taaaatcgaa
    19981 aaagaacagc ttatgacaag gcgttcgtcc caatgatgga atgtcatctg tctaaggcta
    20041 aagggcagga caatggaatt ggttgtcccg ccctcttttt tgaagagtaa ataggaggta
    20101 aagaatggaa aagctgctag ttttttataa caaaagttca gggaaagatg agggtgagaa
    20161 attagcacgc tggtttcaag attatgtaaa agaaaagcag ccagaattat atgtagaact
    20221 agctgaaact ggcccatcga ttgatgatgc ggaattagtc aagaaagcag aagccataga
    20281 agctgatact ttagtaatta taggtggaga cggcacgatt catcatatcg tccaagcgtt
    20341 ccaaaaaaca ttatctcaat accatgtcgg attacttcca ggtggaactg tcaacaacct
    20401 tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct gctgacgtga tcctggaagg
    20461 caatgtacgg aaaatagact atggtcaagt caatgatgaa gtcattatct ctacactgac
    20521 aatcggaatc ttagcagata cagcagtttg gatcagtcaa gaagaaaaac agcgttacgg
    20581 ctcgtggatc tttatcaaga agttttttaa gttattgctg aagaagaaaa aatatcattt
    20641 ggatttagaa acagacgagg agcattggaa aggaaaatcc caactgttga cagtcacgat
    20701 gtctaactcc gtgggcggtt ttacaaattt tgacgactca gcgaccctag atgatggaaa
    20761 atttcatgta acgatcgtac cttccttaga tattttccga tttatttttt atttgcctag
    20821 aatcatgaga ggcaaattct atacgatccc cggcattact tatttcacag ccaaacgcat
    20881 gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg gatggagaca cgacagatga
    20941 tcttccagtt gaattatctg ttgtatcaaa aggattaaca gtttattcac caaagaaaga
    21001 agaaaaagct tagaaatagt agttaacaga aagttggaag aagagtaatg gatgtattgt
    21061 tacacaatct tttgtggttt taccttttca ttgtgaattt ttacttattt tgtctgatgg
    21121 ggtatgataa acatcaggct agaactagcg gctggcgtgt tcctgagaat aatttgatat
    21181 ttgtcagcct gttaggcggt ggtttgggag gattgatctc tcgaaaagtt ttccatcata
    21241 aaactcggaa aaagaagttt actataggat tcgctgcagg tgtacttgtt gatttgattc
    21301 tcatttattt tttccattaa ggagcaggag atgtcgtcta aaggaacgaa aactaaaatc
    21361 attctgaatc tgcttttgct gattgtgatt tttgccatta tttattattt aatcaatcaa
    21421 tcatttgcag atatctttgc cgaattgctg tctaccagtg ttgttgtttt ggcagcggct
    21481 gttatattag ggacagttta ccaaatcgct gaagggcgct caatcaaaga aattgctgaa
    21541 cctttcgcgc cgaattttac aacgaaagat gggttcttga cctctagcta tattgcgttt
    21601 taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg agatttattt ttataacaaa
    21661 aaaggaatga agctatccca gggagcaggg gtgacagcac tccatatgat tatgtacaaa
    21721 ctggcagtga tcacttatgc attagctggc ttggtcatcc aattctctct tttttattct
    21781 aatggtccat caatgatctg gtttatcatc gctggtatcc ttttgacgat cgtgattgtt
    21841 gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt cagcttatcg
    21901 aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa tcagcagatc
    21961 tactctttaa gggaaacggt ccaaaccatt atccaagaca ggactgcgct attgcggatt
    22021 tatctatgga atatgttaaa acttgtattt tggtatgtac ttccttattt gattctgatt
    22081 gaaaaccatc ctaaaataga ttttctactg accatttcgt ttatcagctt tgcagtagtg
    22141 ctttcagggg ttattcctac gcctgctggt attggttcct ttgaatttgt ctatttgtta
    22201 ttattcagac cagttgttgg aacagttgac gcagtttctt cgcttttact gtatcgtttc
    22261 agttccttta tcttgccatt tctctatgga tttgtgtatg tgattgctga acggcgaaga
    22321 gtgatcaaag aagaaataat agaagtaaaa aaagagaata aagaaaatca agggtaggtc
    22381 ttttataaga tctacccttt ttatcaaatc aaagaaaaac gttatggata agataatttt
    22441 acgtttgttt ggctcttttt ttatatacat acaatcaatt ttgttttttt aagatttata
    22501 taagtcgaga aaaaaagtca aaaaaccatg tgtatatttt gaccaaaatt gaccaaagcg
    22561 atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta
    22621 tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca
    22681 acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt
    22741 tttacaacct aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc
    22801 ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt
    22861 ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat
    22921 acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa
    22981 attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt
    23041 gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga
    23101 acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa
    23161 aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg
    23221 aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt
    23281 tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac
    23341 gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga
    23401 agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt
    23461 catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg
    23521 aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt
    23581 agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt
    23641 attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg
    23701 ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt
    23761 atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga
    23821 agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac
    23881 tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc
    23941 aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa
    24001 tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg
    24061 tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga
    24121 acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt
    24181 agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga
    24241 gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga
    24301 acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga
    24361 agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa
    24421 tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc
    24481 gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag
    24541 tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg
    24601 ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt
    24661 gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga
    24721 tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat
    24781 catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat
    24841 tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt
    24901 cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg
    24961 aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct
    25021 gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg
    25081 tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat
    25141 cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact
    25201 tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga
    25261 aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag
    25321 aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc
    25381 tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt
    25441 ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca
    25501 agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagcag
    25561 caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag aactcccaaa
    25621 acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa ggactggttt
    25681 ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga gctgtcagga
    25741 ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg atcaaatgca
    25801 tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa acgattgctg
    25861 ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc aatgcaatcc
    25921 ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt tgtgtgataa
    25981 attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct atttgcacct
    26041 ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac ttctgaaagc
    26101 aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat tgtatataag
    26161 ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt tttcgggtgg
    26221 gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc tttcgcataa
    26281 gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag taaagcaatc
    26341 attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg actgtctgca
    26401 taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga aagctcagaa
    26461 gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact tttagcaaac
    26521 attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa aataatcgct
    26581 aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt cttcatctgt
    26641 ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca tcaagcaagt
    26701 caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga cacggacttg
    26761 tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga caggatcttc
    26821 aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa ggaattcttt
    26881 tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct gactatcttc
    26941 aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt agatcatgga
    27001 aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa gatagaacat
    27061 ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc ttggttcatg
    27121 gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac gccaatactc
    27181 ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa atttgtcata
    27241 aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac ttactgggat
    27301 ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca tgatttccca
    27361 tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc attgcttatt
    27421 cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat ggttagcata
    27481 ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac cgtgaatatt
    27541 gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga accatccgaa
    27601 cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt tattctcaaa
    27661 cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca attcattgaa
    27721 ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa aatctttttg
    27781 atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt ctacaaaaaa
    27841 aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt ggtataatgg
    27901 aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg aaagaacgcc
    27961 aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa tctacattgg
    28021 ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa gatcagctgt
    28081 tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat gctgtagaac
    28141 tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac accccagggc
    28201 acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt gccgttttag
    28261 tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat ttagcgttag
    28321 acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca gcagatcccg
    28381 aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa gctgtattag
    28441 ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg gaatatgttc
    28501 ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac tctatttatg
    28561 atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc aaacccggag
    28621 acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc ggggtcttct
    28681 cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat atcacagcaa
    28741 gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca gacaatcctg
    28801 ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt ggtctttatc
    28861 caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg caattgaatg
    28921 atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg ttccgctgtg
    28981 gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg gaattcaatc
    29041 tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg gatggtacga
    29101 ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag gatgtagaag
    29161 aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca gtaatggaac
    29221 tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat taccgagtaa
    29281 atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac aaattgaaat
    29341 caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa aaaagcaagt
    29401 tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc tttatcgttc
    29461 accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa aaactaatcc
    29521 cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc gttgcccgtt
    29581 cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga gatgtttctc
    29641 gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa caaatcggct
    29701 ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag gacgaaccga
    29761 agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt acatggattt
    29821 gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa atcaagaatt ctttctaaga
    29881 ttttccatga tatcgaccgt ttcatttctc atcttatctg ttacatgcga gtaagtatcc
    29941 atcgtaattg aaattctgct gtggcccaat cgttcagaga tttctttcat ttttgcgcca
    30001 ttttcgagaa gaagtgtagc atgagtatgt ctgagagaat ggaagttaaa agagagggag
    30061 agtgcattcg atatcctcct agtattccat ttcactacac ttggcgtaac tagctcaccg
    30121 tcttccttcg tacatactgc atttgaatca atgtagagct ttccatactt cattcgattt
    30181 tctaattgtt gtttcttatg ttttttcaga attgctagca aggtttgtcc aataaaaatc
    30241 gttcgattgg aactgcttgt ctttggcgta ccatataccc atgcgccgtc attctttacc
    30301 atttgtttct ctacagtaat tgttccattt gaaaaatcga catcatccca cgtcagacca
    30361 caaacttcgc caacgcgcat tcccgtataa aatccaatat tcaaaggaat atagaaagga
    30421 tgtccttcag gagtaatttc tagcatatga tcaaagtcct caagagaaat gatttttaga
    30481 tcttttttag tcgttggtcg tgcttcatat tttggtattt ttacatacag cataggattt
    30541 tgcttgatta atccccaagg ataaaccgcc atattcagcg cattcttaag gacagagtga
    30601 gtaatagtca ttgttttctt cgagtaaccc tttttaaatt cagcattgat gaaattttgt
    30661 aaaagagcag gggagagatc cgtaagtttt ttctttccta aataaccgtt tatatgattt
    30721 ttgatggtaa atcggtagtt ttcataggta ttgtatttta gatttagttt aacgtattcc
    30781 tccatccaaa aatcaaggta ttgttttact cgagtatccg tacctaaaaa gtattgtcct
    30841 gtttcgtcaa tatctgataa aacttttcgt aaagcagctt cggcctctgg tcgggtgtct
    30901 ccgccaactt tctccacttt ttttcttgag ccatcatcat tgatatct
//
LOCUS       DACEJZ010000025        30416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEJZ010000025 DACEJZ010000000
VERSION     DACEJZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.025"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1273..1938)
                     /locus_tag="IW656_001059"
     CDS             complement(1273..1938)
                     /locus_tag="IW656_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0836779.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IW656_001060"
     CDS             2064..4100
                     /locus_tag="IW656_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0836780.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IW656_001061"
     CDS             4104..4742
                     /locus_tag="IW656_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836781.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IW656_001062"
     CDS             4770..5198
                     /locus_tag="IW656_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836782.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IW656_001063"
     CDS             5655..6566
                     /locus_tag="IW656_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0836783.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IW656_001064"
     CDS             6783..7220
                     /locus_tag="IW656_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836784.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IW656_001065"
     CDS             7315..8199
                     /locus_tag="IW656_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0836785.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IW656_001066"
     CDS             8423..11590
                     /locus_tag="IW656_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836786.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IW656_001067"
     CDS             11610..12155
                     /locus_tag="IW656_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0836787.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IW656_001068"
     CDS             12183..12638
                     /locus_tag="IW656_001068"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0836788.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IW656_001069"
     CDS             12635..13393
                     /locus_tag="IW656_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0836789.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13479..14219)
                     /locus_tag="IW656_001070"
     CDS             complement(13479..14219)
                     /locus_tag="IW656_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836790.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14280..15533
                     /locus_tag="IW656_001071"
     CDS             14280..15533
                     /locus_tag="IW656_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0836791.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            15855..16082
                     /locus_tag="IW656_001072"
     CDS             15855..16082
                     /locus_tag="IW656_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0836792.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16205..16531
                     /locus_tag="IW656_001073"
     CDS             16205..16531
                     /locus_tag="IW656_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0836793.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            16599..17312
                     /locus_tag="IW656_001074"
     CDS             16599..17312
                     /locus_tag="IW656_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0836794.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            17622..18473
                     /locus_tag="IW656_001075"
     CDS             17622..18473
                     /locus_tag="IW656_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0836795.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            18594..20459
                     /locus_tag="IW656_001076"
     CDS             18594..20459
                     /locus_tag="IW656_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0836796.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            20507..21916
                     /locus_tag="IW656_001077"
     CDS             20507..21916
                     /locus_tag="IW656_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0836797.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22024..22725
                     /locus_tag="IW656_001078"
     CDS             22024..22725
                     /locus_tag="IW656_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0836798.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            22884..23363
                     /locus_tag="IW656_001079"
                     /pseudo
     CDS             22884..23363
                     /locus_tag="IW656_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            24211..24786
                     /locus_tag="IW656_001080"
     CDS             24211..24786
                     /locus_tag="IW656_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0836799.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            24786..25226
                     /locus_tag="IW656_001081"
     CDS             24786..25226
                     /locus_tag="IW656_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0836800.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            25242..25943
                     /locus_tag="IW656_001082"
     CDS             25242..25943
                     /locus_tag="IW656_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0836801.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(26431..26856)
                     /locus_tag="IW656_001083"
     CDS             complement(26431..26856)
                     /locus_tag="IW656_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836802.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            27230..28201
                     /locus_tag="IW656_001084"
     CDS             27230..28201
                     /locus_tag="IW656_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0836803.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            28233..29630
                     /locus_tag="IW656_001085"
     CDS             28233..29630
                     /locus_tag="IW656_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0836804.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            29789..30412
                     /locus_tag="IW656_001086"
     CDS             29789..30412
                     /locus_tag="IW656_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836805.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT        10323 a         4935 c         6264 g         8894 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt
    13681 tttaaagaag tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc
    13741 aaacctttga tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct
    13801 ttttgatcaa taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt
    13861 tctagcactt gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga
    13921 aggattttcc gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata
    13981 ttactatgtg catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc
    14041 ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac
    14101 tgattgattt gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat
    14161 tctctgatga tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata
    14221 tgcaggctcc tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga
    14281 tggtcgcaac agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt
    14341 tattcttttc gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg
    14401 tgacactttt acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga
    14461 tgtttctttt ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt
    14521 tttttgctat cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa
    14581 agagacatct tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat
    14641 tagtaaaaag tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg
    14701 aacactattt tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg
    14761 gaagtgttca cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca
    14821 gttttttttc tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac
    14881 atcaggaatc gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat
    14941 tcaaacatga ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa
    15001 aagaaacgtt ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac
    15061 cgcaaataca acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat
    15121 ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga
    15181 aaacaatccc gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg
    15241 cattcgatca tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag
    15301 agggatcacg ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga
    15361 tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa
    15421 aaatgttgga tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact
    15481 atttttttgt agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg
    15541 tcaaaggata agtttgagga aagtctacac ggagatagag acaacaacac agcatattca
    15601 aaagaaacag aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa
    15661 caaagtagtt gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa
    15721 gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca
    15781 ccagataaag ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta
    15841 cgatcacaaa aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc
    15901 atcgaaaagc ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa
    15961 aatcattaag agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac
    16021 aaaaagcctc aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt
    16081 gattttctat gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc
    16141 tattttttat gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa
    16201 gcaaatgaat agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt
    16261 tcataacgaa aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact
    16321 ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga
    16381 actggtagat gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga
    16441 tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc
    16501 aggtgtacga aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc
    16561 aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa
    16621 aaagatggct agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt
    16681 tcaatgaaca gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga
    16741 atgtaacaaa agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg
    16801 cggtagaacc aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac
    16861 cagtgattgc tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct
    16921 tggataatac gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa
    16981 aaggaaatta tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc
    17041 tagaaaattt agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat
    17101 acaaaacatt ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg
    17161 agggaaaaga aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag
    17221 ttgtacaagg agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat
    17281 ctgctttcaa tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt
    17341 aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa
    17401 cgcaaagagg tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt
    17461 tattttttat atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata
    17521 agaaatggat tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg
    17581 aattctaggt ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
    17641 tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa
    17701 aaggactagc ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag
    17761 aatttgtttt aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc
    17821 cggcagagga gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag
    17881 ttgaattttc gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac
    17941 gagcaaaaga aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc
    18001 cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg
    18061 aactttctga ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa
    18121 acgaagacat gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca
    18181 tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta
    18241 ctcatctgca gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg
    18301 aatttctcta cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga
    18361 tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt
    18421 taacgattca tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt
    18481 tactgtatgg caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt
    18541 tttttcaatc tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata
    18601 atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt
    18661 tagtacattg tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc
    18721 aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag
    18781 tagtgattgg cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg
    18841 cagtttcgga ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta
    18901 tctctagtat atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat
    18961 tcttatcatt ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat
    19021 tgtttgctgc agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag
    19081 ctaaaaagtt caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc
    19141 cagctatcac agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga
    19201 ttctttcacc atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc
    19261 aaagtaaatt agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag
    19321 ttccgcttgt cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg
    19381 ggacggttgc cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa
    19441 tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt
    19501 ggggattcgt accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc
    19561 caatgcttct tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa
    19621 caaaagatac gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa
    19681 tcactgaacc aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt
    19741 gtatttctgg aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca
    19801 gcagtttggt cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt
    19861 caaatgtgac agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta
    19921 cattgatttt aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga
    19981 atgcaggaga accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag
    20041 tactgccatt gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta
    20101 tcgctattga tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta
    20161 tttttccaac tggacatgct gtagggatca caacgactga cggagcggag attttgattc
    20221 atatcgggat ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag
    20281 gggatctcgt caaagcagga gatttattga ttcgttttga catcgaagca attagagcag
    20341 ctggctatag cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac
    20401 ttgaattaga ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag
    20461 gaggaaatca ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct
    20521 gccaaatcaa ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc
    20581 tatgcctgga ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat
    20641 tgatcaagaa aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa
    20701 agaagatctt gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg
    20761 gacacgaatt tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta
    20821 tgatcagctg attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca
    20881 ttacgaaatg cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat
    20941 cgacttctat gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata
    21001 ttggatgact ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt
    21061 agtaaaaagc aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt
    21121 cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg
    21181 ctgcatgatt atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc
    21241 aacaatgatc caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga
    21301 ataccctgca tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga
    21361 gcaaacggaa aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag
    21421 ttattatatg tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa
    21481 cttgctggga ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga
    21541 tccagaaggg ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt
    21601 cattgttgaa aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga
    21661 tgattatcgg atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga
    21721 cggtgtagat ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac
    21781 aggagaaatg agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg
    21841 gacactgaat cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa
    21901 tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt
    21961 tgtgttcttt ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac
    22021 gttatgaaaa ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt
    22081 ttctcaggaa aatatccagt cggttcgatg ttgccgacag aaagtgaatt agaagaactg
    22141 tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc agatgaatat
    22201 gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca atcgtcctta caatcgatta
    22261 tccaaagcgg caagctttac gcaaatcttg gagaactccc gatatgaagt ccggaaagaa
    22321 acgctggatt tgcaattggt ccatataaag aaggaagatc ctttgttttc ctttttcggt
    22381 gagcaagctg tttgttttaa gcgcttatat tatttgaaca atcagcctta tatttatttt
    22441 gttcattatc ttccacaaga aatgatgcaa ttcagcagag aagaatttgg caaagaatct
    22501 ttgtatcgtt tgctgacgaa aaaaggatat actattgact cattttctga tgatttctcg
    22561 gcagtatttt taacagaaga agaacaagga attctgaaaa cagacgcaca gatcggcatc
    22621 aaacggatac ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc
    22681 taccatacgg agctgcatcc ttaccatatt gattatgaaa cataaataaa aaaagaggtt
    22741 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    22801 ttttcgtttg gttaggctta ttaccgcaga tacacggctg tttctggatt accatttatt
    22861 ctggataagg gggtgtggca cgattttcag aaggaatcta tgaaccaatc actgtattga
    22921 ttttagcttc gatggtaacc ccattcggct tgacgatcgc ttatttctta gggaaaatca
    22981 ttcgtaaaaa tatactcaat cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg
    23041 ggatctgtca gatcacagag ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg
    23101 tcatagcaac gggagtcggt gggacagtag gtggcggatt aagcatgttt tggggcgcag
    23161 atagccgtat tccggcatcg ggaatgtttg cagtcgctac gatgaccaga ccatgggcat
    23221 tcattggtgc attgttagct ggttcttttg tcacaggaat aaccatttta ttattgaaaa
    23281 aaccagtaga tccgaatgca gaaatcatcc aagaagaaaa agaggaagaa gacatatctt
    23341 gggatgattt gacaattagt taggagaggc agaatggatt acaagaaaaa agtacaatag
    23401 atgtaccttg agacaggaat ggtatgaaag aatcagagtt agaaagaatc gaatatacag
    23461 attttggttt aggagcgatc gaaactgtcc agagcatcgg caccctcctt gaaatgacga
    23521 tgaaggaaaa atgagaacct ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga
    23581 aaggaaacaa aaaaactttc ctgtactatt ccaaaaagga aagaaagtta ttatacagta
    23641 aaaaaagaaa ttattcttca tgctagagat cagtacacgt tcgagcccaa tatcaaacac
    23701 tagttccagg caggcgcaac aggtgcggtt atcactgaat tttcttccag cagtgacgat
    23761 tcaagtgata tttttacaaa tccagctatt tatcgttaac aaaaagattt ttaggagaag
    23821 ctgatgaaca aaaatgatca aattttgatc gtttagttcg tcggcttttt tatttagaga
    23881 aacatcattt atcagtgaaa cataacacga gaacaaaatg attttttcgt taataaaaga
    23941 aacaagtatt tgttgtttaa aataatacac gttattggca tataggacga aatcccagta
    24001 tcttctataa gtaagtcatt tcatatcgat gaaaacacgt tatttatttt tgttttatct
    24061 ataaaatggc tgaaaaatga tttttttcta aatactatat atagtggcgt ctatcctata
    24121 taccactaaa aaactacaaa atctagtctt ttattttaaa aaaacacgtg tagaatgaag
    24181 atagactttt gagttaggga gtgttttttt atgaaactta ctgttttttg cggttcccgt
    24241 tttggtaata aagaaagtta caaatttatt gcgcaaacgt taggaaaata catggcacaa
    24301 gaagaaatcg aactggttta cggtggttca tactccggga ttatgggagt gattagtaaa
    24361 acagtactgg aaaataacgg aaaagtcaca ggtatctatc cgaatgggct ttttgaaact
    24421 gaattgcctg gaaaagatgt tactacattt attcctacag aaacgatcga tgaaagaaag
    24481 gtattattat ttgaaaaggg agatgctgtc ctcgttttcc ctggaggtct aggtacattg
    24541 gaggaattct cacaacttct ttcttggata gcaatcggtc tgacgcccga caagccaatc
    24601 ggaatattga atattggcgg ttattacagc ggcttgcaac aattattaga aacttttgca
    24661 aaagaggatt ttatggataa aaaatggttg gatcaagtaa tcttttccaa caatccattg
    24721 gaactggtca atgaattacg agcagtatct attaaaaatc agttattatt gaaggaggca
    24781 aaataatgag tcaaatatat ttagcaggac catttttttc agaggagcaa atcgatcgag
    24841 taagtcgtat cgaaaaagcg ttggaggaaa acaaaacagt cacaagtttc tactctccac
    24901 gtcatcacca agaaagcaat tatgaactat tcagcgctgg atgggcgcag gaagtatacg
    24961 aaaaagatat ggaagaactg acaaatgcag aatttgttgt tgctatcctt gactttgagc
    25021 atcaaacaat cgatccagga acggcttacg aattaggtgt ggcaactatg ttgaaaaaac
    25081 cgatgatcat tgtacaagaa gagacggtac ctacgaactt gatgattaca caaagtctcc
    25141 atacgtattt aaaatcagac caagctgtac gtgaatatga ttttgaaaca ttgcctgttg
    25201 aaacttacgt aggcgagtac ttatagaaga ggaagggttg tatgtcagtg attttgattt
    25261 caggaacgat tggtgcaggt aagagcagtt tgacggatat gcttgcaaaa gagattgatt
    25321 ctaaaccatt ttatgaaaat gtcgaagata acgaagtatt gccgctcttt tattcaaatc
    25381 ctgagcagta cgctttttta ttgcaaatct tctttttgaa caagcgattt ttagcaatga
    25441 aaaatgcttt agtgaacgat gacaatgtgc tagaccgatc catctatgaa gatagcctgt
    25501 tgttccatct aaacgctgat ttaggtcggg taacggatat cgaagtacag cagtacgata
    25561 gcttattaga tacgatgcta aacgagttag atgatgttgc tccaaaaaaa cggcctgatt
    25621 tgatggtcca tatcaaagtt tctttggaca cgatgctaga acgtatcaaa aaacgtgggc
    25681 gtgactatga acagctagaa agcgatgaga cgttatatac ctattatgaa acgttgaaca
    25741 cccgctataa tcaatggtat gaagattttg atgtatgtcc taaaatacaa gtggatgggg
    25801 ataaattcga ttttgtagaa aatccagaag atgcaaaaaa ggtgatccgt cagatccaag
    25861 aaaaattagc tgaattggaa ggaaagcctg ctggaaaata ttttacggat aaccagcatg
    25921 caaaaggtca agaattaggc taaaaataaa aaaccaaacg acgggaaaga aaaattacta
    25981 cgtgatgtag aataagcctt tctatcgttt ggttatttta tattcaaata tttgaaataa
    26041 ataaatcttt actctttgat ttgtaaactg aaatgatcca ctgtttgttt tactgatgaa
    26101 gcttgatcaa aggaagaagc tgagtttgaa gttcataaaa atcagccata gtgagttttg
    26161 cttggatttg tttatcttct tctacttttt acataatttt gaacaggata agattcaaat
    26221 gttttaaatg aatctttgac tagtttgtga cagttgtcgc atatgctcaa gcttccttgg
    26281 tcattaaatt ctgtatggat atttttgttg ttattacatg tacaataatc aggactatcg
    26341 atcatccatt tcgtctcctt tacaatcatc ttgcttcttt actatagcag aaaagaaggg
    26401 gagaaacaaa agaagatcca acagacggca ctatagactg tagatgttgc catcctcatc
    26461 cttcaaacgg taattaccga tttttgtaaa tatgactgtt gtttcttttt tattttcttt
    26521 tcctgaaata atatattcgt catctttggt agtgatcttg atatctgtca gcactgtttt
    26581 ttcttcagat gattcagaag aatcagttcc ttgtgtctga tacgtgcgtg tccattccat
    26641 ttttttatct gtaaatgtca actgtcccgc ggataaatcc atatctttac gtacttttag
    26701 cgtatataca ctgttggtgg tatctgcttt ttccgctttt aagacagaag aatcggttgt
    26761 ttcgtccgag gattggtcag agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc
    26821 cgcagacaaa ataaatagta cgattaattt cttcaaaata cttcccccat ttgataggat
    26881 aaagaaatta taccattgga atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa
    26941 acacccacaa atagagtaaa ttttagaata aaattttaaa aaggcaaaaa atccgccact
    27001 ttcagaaagc gttttaaaaa agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga
    27061 accggttcaa atacttgttt aagtataatt aatagtgaga ggaagttgct ctactgaaga
    27121 ctttagttat atggatattg atgacaagca acactatgca aagaatgcgg tcatcataat
    27181 cgaacaacaa gaaagagaaa tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat
    27241 ccgtgatgtg gctaagatgg ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg
    27301 tcagcaactg aaggaaaaga acatgaaaaa aatagcagaa gcgatttcag cgttggacta
    27361 taaagaaaac atcattgcta aagggttgaa gaataatcga agcttctcta ttggactttt
    27421 gataaacggg atttccagcc gttttgggtc ggaagttgtc tcagggatcg aaagagtagc
    27481 tgaagaaaat gggtatagct tattattaag cgggtttgcc gatcagccag aaaaaattga
    27541 acaaaaaatt gaatacctga tgaagcatgt gatcgatggg ctgattgtct tcttatccga
    27601 agaagagtgg tctggatttg aaatgctcac aaaaatgagt attccagtta tcgcattgaa
    27661 ttgtccagaa ggaccagcag gtattgatac aatattagtg aatgatcgag aaagtgtagc
    27721 aaaagtgatc gctcatcatg gtgaacaagg acataaaaaa atcggattta ttgctgccac
    27781 tcaaacagac tacgtagcaa gagaacgttt agcaggagcc aaagaagcgg tccaaaatga
    27841 tccatcgatc caattagagg tctttacagg agactattct agaaaaagcg gctattacgg
    27901 ggcaaaagca ttattagaaa aaggggcaac tgctattttt gtcagcaatt acaatatgtc
    27961 gattggtgcc atcgaatgtt tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt
    28021 cagtcactat gattatttag ataaaaacga tctgagtatc cttccgaaaa tcacgatcac
    28081 gccaccaaca gataaaatcg gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa
    28141 agaagaggaa gaaaatgaaa aagtgattgt gatggaaaat atcatacatg gaatcaacta
    28201 aaacgaacta aaaaagaaaa agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt
    28261 tttggtgggg ttctgcagca agtggcccac aaacagaagg aaggatttca ggcgatggaa
    28321 aaggagagaa catttttgat tattggtaca aaaaagaacc gcaaaagttc tttgatcaag
    28381 taggacctga aaaaacctcg caagtttata cgaagtatca agaggatgta cagttgatga
    28441 aacagactgg gcataattca tttcgaacat ctatccaatg gagccgtttg atacctgatg
    28501 aatctggaac aatcaacgaa gaagccgttc gtttctatca tcgttatttt gatgagttga
    28561 ttgaaaacgg tatcgaaccc tttgtgaatc tgtatcattt cgatatgccg atgtatctgc
    28621 aagaaaaagg cggatggttg aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa
    28681 gttgttttga attgtttggt tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg
    28741 ttccagtcga agcaggttat ttatatcgat ggcattatcc agaagagtca gatatgaaaa
    28801 aagcgatcca agttggctat catgaagcgt tggcttcagc gttagccatc aaatcttacc
    28861 atgaaatgga gcaaggtggt gttattggta tcatcttgaa tctgacacca agctatccaa
    28921 gagacgaatc aaaccctgaa gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc
    28981 gttcattcct tgatccagct gtaaaaggcg agtttccaag tgaattgatt gagatagtaa
    29041 aagaacttga tctcatgcct gtgatccatg cagaggattt gacggttatc aaagaaaata
    29101 cagtagatct tttaggtatc aattattacc agccacgccg ggtaaaagca aaagaaacac
    29161 caatcgatac taaaaacggt ccaatgcctg aggattattt cgacaattat gaaatgcctg
    29221 gtcgtaaaat gaatccttat cgcggatggg agatctatga acaaggaatc tatgatattt
    29281 tgatcaatgt acgagatcat tatggaaata tcgactgctt tatttctgaa aacggtatgg
    29341 gtgtagaaaa cgaagcacga tttatcaaag aagatggcat gatccacgat gattatcgga
    29401 tcgaatttgt ccaatctcat ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt
    29461 gtttaggcta tcatatgtgg acatgtatgg ataactggtc gtggaccaat gcatataaga
    29521 atcgatatgg ctttatttca gtagacttag cacaagatgg aaaaagaaca atcaaaaaat
    29581 cgggtcattg gttcaaagac gtatcagata aaaacggatt tgaggcttaa gaaagaatag
    29641 ttgagagcaa atgcgtgaaa ttctccattc ttaccatttt gtgaaaaaga agttcaatta
    29701 tttacgatct ttttcaactt ttttgtcttt ctatttcatt ttttgtatat aatagaatag
    29761 tcaaaattaa ataggagtgg aggaaagcat gaggaaaaaa agaccgtttc ttctcttttg
    29821 gttattcggt tttttcttac tgcttatttt atccgcagca ttttcaggaa aaatgacagc
    29881 agatcttgct cagaaagaaa cagaaaaaat cgtagcaagc aaagaatcag tagataattt
    29941 caaaaaagaa ttctcaactt attggtgggc ggagtataaa ataactgcca attacgaaaa
    30001 aggcgaattg gacatttatt tgccaaaagg aaaagaagaa aatcaattta ttaatatcga
    30061 tgacgtactt agtgcgattt ctctcagaca atatcaattt aaagacaagc atttgaccgt
    30121 tttgcttttg actcatgatg gcgacaaagt agcccgtttg aaaaatggaa aagtgatcac
    30181 cgcttattct gaaaaagtag acaaaaaagt ggtcgccgat cgtttagcgg aatggaaaaa
    30241 gacaaatgaa caagtcaaga aaacaactgt ctcttctcaa aaaaacgagt cgacaggaac
    30301 aagtccttca gattctggca gtggacaaac gataacggga agtgaagaaa atcaagcctc
    30361 aattattacg aatgagtcat cacaaggaca atatcaatcc tatggacaat gaaaaa
//
LOCUS       DACEJZ010000026        30386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEJZ010000026 DACEJZ010000000
VERSION     DACEJZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.026"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(55..2130)
                     /locus_tag="IW656_001087"
     CDS             complement(55..2130)
                     /locus_tag="IW656_001087"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0836806.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2132..3049)
                     /gene="glyQ"
                     /locus_tag="IW656_001088"
     CDS             complement(2132..3049)
                     /gene="glyQ"
                     /locus_tag="IW656_001088"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0836807.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3435..4247)
                     /gene="recO"
                     /locus_tag="IW656_001089"
     CDS             complement(3435..4247)
                     /gene="recO"
                     /locus_tag="IW656_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0836808.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(4390..5289)
                     /locus_tag="IW656_001090"
     CDS             complement(4390..5289)
                     /locus_tag="IW656_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0836809.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5304..5705)
                     /locus_tag="IW656_001091"
     CDS             complement(5304..5705)
                     /locus_tag="IW656_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0836810.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(5683..6159)
                     /gene="ybeY"
                     /locus_tag="IW656_001092"
     CDS             complement(5683..6159)
                     /gene="ybeY"
                     /locus_tag="IW656_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0836811.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6188..8380)
                     /locus_tag="IW656_001093"
     CDS             complement(6188..8380)
                     /locus_tag="IW656_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0836812.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8404..9375)
                     /locus_tag="IW656_001094"
     CDS             complement(8404..9375)
                     /locus_tag="IW656_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0836813.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10081..11460
                     /gene="brnQ"
                     /locus_tag="IW656_001095"
     CDS             10081..11460
                     /gene="brnQ"
                     /locus_tag="IW656_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0836814.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11616..12062)
                     /locus_tag="IW656_001096"
     CDS             complement(11616..12062)
                     /locus_tag="IW656_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0836815.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12089..12265)
                     /locus_tag="IW656_001097"
     CDS             complement(12089..12265)
                     /locus_tag="IW656_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0836816.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12427..12855)
                     /locus_tag="IW656_001098"
     CDS             complement(12427..12855)
                     /locus_tag="IW656_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0836817.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12947..13807
                     /locus_tag="IW656_001099"
     CDS             12947..13807
                     /locus_tag="IW656_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0836818.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13902..14195)
                     /locus_tag="IW656_001100"
     CDS             complement(13902..14195)
                     /locus_tag="IW656_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0836819.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14268..15995)
                     /locus_tag="IW656_001101"
     CDS             complement(14268..15995)
                     /locus_tag="IW656_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0836820.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(16209..17135)
                     /gene="trxB"
                     /locus_tag="IW656_001102"
     CDS             complement(16209..17135)
                     /gene="trxB"
                     /locus_tag="IW656_001102"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0836821.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17280..18713)
                     /gene="ascB"
                     /locus_tag="IW656_001103"
     CDS             complement(17280..18713)
                     /gene="ascB"
                     /locus_tag="IW656_001103"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0836822.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18713..20116)
                     /locus_tag="IW656_001104"
     CDS             complement(18713..20116)
                     /locus_tag="IW656_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0836823.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20346..21248
                     /locus_tag="IW656_001105"
     CDS             20346..21248
                     /locus_tag="IW656_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0836824.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21289..23175)
                     /locus_tag="IW656_001106"
     CDS             complement(21289..23175)
                     /locus_tag="IW656_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0836825.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23271..24122)
                     /locus_tag="IW656_001107"
     CDS             complement(23271..24122)
                     /locus_tag="IW656_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0836826.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24579..26363)
                     /locus_tag="IW656_001108"
     CDS             complement(24579..26363)
                     /locus_tag="IW656_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836827.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEKYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26363..28114)
                     /locus_tag="IW656_001109"
     CDS             complement(26363..28114)
                     /locus_tag="IW656_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0836828.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28256..28480)
                     /locus_tag="IW656_001110"
     CDS             complement(28256..28480)
                     /locus_tag="IW656_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0836829.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28731..30317
                     /locus_tag="IW656_001111"
     CDS             28731..30317
                     /locus_tag="IW656_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0836830.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8420 a         6561 c         5441 g         9964 t
ORIGIN      
        1 ccagagaaat agcctatttt gtgcctgttc tctggtttat ttacatatta gaaattattt
       61 tgtgatgatt tgatctaaac ttgctagtga caaagccatg gcagcaattt gacttaattg
      121 cgttaatcgg ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata
      181 attttcgatc agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc
      241 agcaaagctt gttttcattg cctctactgc tttgtgcagt tctttctccg cttcgttttc
      301 gaacagttca gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac
      361 gacacgtgtc aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa
      421 caattgtgct gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc
      481 gatgacatca tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa
      541 gtcgatcacc tgttgttgtc ccgctgacaa gccaatgcca aaacgatccg catctttact
      601 gataacttca ctaatcgttt cttgcagaac agataaagga aaggcccatt ttttcgattc
      661 gatgattcga acgacaccat atgcttgacg gcgcaaagca tatggatcgt ttgagccact
      721 tgggatcatc ccaactgcaa agaaagaaaa gatactatct aatttatcag caatcgctaa
      781 aacagaacct actgtagtct caggtagatc tccttcactg gatataggca tgtaatgttc
      841 acggattgct gttgctacgg ctggtttctc gcctttaagc agagcatatt tttctcccat
      901 gatcccttgt agttcaggga attctcctac catgttggtt actagatcaa atttatagat
      961 ttcagcagcg cgttgcagat ccatcaattc ttcctcagat agtcctactt gctcgcctat
     1021 gatgcgggcg atgacttgta cccgttgcat tttctcgtag atgctaccga ttttttcatg
     1081 gtaagtaaca gtttttaatt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt
     1141 ataaaagaat tccgcatctt ctaaacgagc agtcaatact ttttgattgc ctcgaaccac
     1201 attttccaat tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc
     1261 ttgatcattc cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc
     1321 ttcaggaaca gaaagatatt tttcttcgta gtcgccagaa aagacagttg gatattcgac
     1381 taaattgttg acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc
     1441 tgctaattcg tttgcttgtg taacgatcat ttcttgtctt ttttgcggag acgcaatgac
     1501 gaattgttca ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc
     1561 taagaaacga tgacctcgag aggctctgcc tgttttgaca tccaagattt cgaatgggat
     1621 tacttcatca tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta
     1681 ttctgcccaa tgcatcgtta cagggaaagt caggctttta atgacatctg ctaaacctgt
     1741 caatacttct ttggattcct taccatgagt gtatttcgtt acatagacat attccacacc
     1801 ttttaattct ctaaaagtga tagcgtctgt cgtcagacct tgtccgcgga caaatccttc
     1861 cgctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac
     1921 ttcttcttca ctgtccgctt gtcgttctgg aatatttgtt actttgattg ctaaacggcg
     1981 cggtgtggaa aatgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc
     2041 tgttttttct tctaattgtt tcatgcttgg tgtgacaaca tgggcaggca tttcttccag
     2101 cccgatttca agtaataagt cttttgccat cttattcagc ctccttcacg gtttctggat
     2161 ttgctgtgtt tttattcaac aaagggaatc ctaatttttc gcgttctgcc acaaagattt
     2221 tggctacagc acgagccatg ttgcggatac gggaaagata accggcacgc tctgttacgg
     2281 atactgcacc gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat
     2341 acgctggatg aacaagattt tcatcgatac agcgtttagc ttctttttca aaacgatcaa
     2401 agttgcttag tagcaagtct tgatcgctta cttcaaaaga atatttggaa tgttcatatt
     2461 ctggttgttt gaagatctcg ccatatttca cgccatcagc ccattctaaa tcatagacac
     2521 tttcaacttc ttggatataa gaagcaagac gttctagtcc ataagtgatc tcagaagtga
     2581 ctggtttaca aggcagtcca ccaacttgct ggaagtacgt gaattgtgtg atttccattc
     2641 cgtcaagcca tacttcccaa cccacaccgg cacagcccat ggatgggttc tcccagttgt
     2701 cttcaacaaa acggatgtcg tgttctaatg gatcgattcc taaaaggcgc aagctttcta
     2761 aataaagctc ttggatattc tcaggtgaag gtttcatcac gacttggaat tgatgatgct
     2821 gatacaaacg gtttgggttt tcaccataac gtccatcagc tggtcggcga gatggttcta
     2881 cgtaagcagc attccatggt tctggtccga ttgcccgtaa aaaggtatat gggctcatcg
     2941 tacctgctcc tttttctgtg tcatacgctt gcatcagcat gcagccgttt gatgaccaaa
     3001 atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc
     3061 ctttcaattt taaagcgttg ctgtcaaata agaatacagg aaagaaaacg acaaaaaatc
     3121 cctatgtctg attacagaca tagggacgaa taattcgcgg ttccacccta cttccgaata
     3181 aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc
     3241 ggctcccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac
     3301 gtattcattt tgcagttttc ttgtttctat aatagatcct ctcactgaac gatctatttg
     3361 atctacggtt aataatagac ggagctccct gtgtttgtca aatggttttg attttttgtt
     3421 gtatgtttca tttactattc ttctttttta ggtagatctt tttcagacat gtctttctct
     3481 ggcgtcttta atacgtgtgc ccaagacttc atttgatcga tgaacttttt gcttttcaaa
     3541 tgaataccga catattcatc atacagctgg tcgatgagtt cacggattgc ttgtttggtt
     3601 tcttctttca gatggattcc actgatttta tcataagaaa tagctgaaaa catccgaacg
     3661 aaataaacag ctcgaggatc tgcatgataa cggtgatcgt ccatcgacca atgtctttcg
     3721 catatcacac caccatattt ggaagaatag tcaaattttc cttttgtttc cccacatacg
     3781 acgcagtgtg tccagattgg tgcaattccg aagcgttgca gtatttgaac ttcaaagata
     3841 ttcgtgatga tttctgcatc atctccttga tccatccgct gcagagcttg ttgcgtaaat
     3901 tggaataaat gcgggtcata ttgatgatct tcgattgctg catctactag atttagcaaa
     3961 taggtaccat aaccgctcaa aaagatatct tcttggatgt tccgaaacgc atggacgtct
     4021 ttgacactgc ttaaaaacga caaaccttca cttcgaaaat taccgatata gactgcttcg
     4081 gtaaagggga gcaaagccgg agctaatggc tgattttgcc gatggattcc tttgatataa
     4141 aacatcattt tgccagaaga ttccgtaaag attttgacta atttatcttt ttctttgtaa
     4201 ttacggctag ataaaatcaa gcctttcgat tctgcaagct ggctcatatt ctccctccta
     4261 aaaaataaat ctggaaaagc agattctgcc gatccagatt atttatgttt cgatcttccg
     4321 ccgtttttct caacgtgcgg atttttatcc agtttatttc tatacttaac ctataagaag
     4381 ttcagaatat cagtattcat cttttcggta gccgaagtct tgcagataga cttttttgtc
     4441 tcgccagtct ttttggactt tgacccaaag ttctagaaat actttgtctc ctaaaagatt
     4501 ttcgatatcc tttcttgctt tcgttcctac ttgcttcagc atttttccgc ctttgccgat
     4561 aatgattcct ttttggctat ctctttcgac gatgatcgtg gcctggatat ggattttatc
     4621 attctcgttt cgcttcatcg aatcaacaac gactgcgaca gaatgaggca cttcgtctcg
     4681 tgttaaaaac aagactttct cacggatcaa ttcagaaacg atgaagtatt caggatgatc
     4741 agtcacttga tcatctggaa aatattgtgg gccctctggc atttcactga ctaatacatc
     4801 catcaaacgt tcgacgttgt ttccttctgt agcagaaatt ggaatcactt gtgcaaaggg
     4861 catctgcgtt gaataatctt cgatgattcc caacaactcg tctggatgga ttttatcgat
     4921 tttattgata atcagataaa caggagaatg cacgtttttt agacgttcaa taataaaatc
     4981 gtctccccgg ccgcgttttt gatcagcgct gatcataaat aagaccgcgt ccacttcttt
     5041 caacgcactg taagctgctt ctaccataaa atctcctaaa cgatgctttg gtttatggat
     5101 tcctggtgtg tcaataaaaa tcaactgagc ttctggtgta gtatatactc cttggatttt
     5161 atttctagtt gtctgcgcct tatcactcat gatggcaatt ttttgaccca cgattcgatt
     5221 caataatgtg gattttccta cattcgggcg gccgacgatt gcgacaaacc ctgatttatg
     5281 ttctggcata tacgttctcc tttttaatta aacaacaaat gatatatttt agggacaaat
     5341 aaaatcaacc caacgatgac ggcaaaacaa gcggtcacta aaactgctcc agctgccata
     5401 tctttgattt ttttcccaat cggatgaaaa tgaaaatccg taaacatgtc tacgacattt
     5461 tcaaaaacag tattgatgat ctctactatc catacgagaa aaacagctaa aagcagccat
     5521 aaccattcta tccgatcaag ttggaaaatc acgcccataa acaaagcgag gattccgaac
     5581 gcaacatgtt tcctcatgtt gcgttcttcc tcaaaaacag ttttgactcc ttggacggca
     5641 aattccacgg acatcaggaa atgtttgttt ttctctacgt ttttatcttt taagtccata
     5701 ggcatctaag atctctttct gtaaaccaaa catttctttc tcatcctctg gtgtcatatg
     5761 gtcatacccg ttgatatgca agaatccatg aaccgctaaa aagccaagtt cccgatcata
     5821 cgaatggccg taagcttctg cttgctctgc agcacgttca gtagagatca tgatgtctcc
     5881 cagtacacga ggcaatggat tctctccatc ttcaaaaatc actgggattt cgtcttcacc
     5941 ctcttcttcc aaggcaaaac tgatgacgtc tgtcggtgca tcttttccac ggtaatcacg
     6001 attgattact tggatcgctg cgttgtccat gaaagtaaca gacatttctg tttcttctgg
     6061 caactctaaa tatgatgctg caaattgcaa caagtcgtcg atttctttca ttttctcttc
     6121 tgagacttgg tttgtttcat ccatgaaggt aatatccatt atacatcgct tctttctatt
     6181 cttcttgcta ttcttcttgt tcttctttta tcttctcaac ttccgatttc attttaggat
     6241 atttgatccg agaatggaag gtactgctta aaccgctgat caatgatttt tctactttac
     6301 ggatttcttt taacgtcaat ccactgtcat ccaattgtcc atcggaaata cgctcttcta
     6361 taagattatg gacaaaactt tcgatttttt cgcttgttgg atggtccatt gcccgtactg
     6421 ccgcttcaca actgtcagct atattgacga ttcctgcttc acggctttga ggttttggtc
     6481 caggataacg gaattgctct tccgtgactt ccgggttgcg ttcctttgcc ttaacgtaga
     6541 aataacgcat cagcgtggtt ccatgatgct gtctgcaaat atcgatcacc atttgcggca
     6601 tattataatc ttccaatatt ttggcaccat ctgtcacatg tccaaagata atatgtttac
     6661 tgtcttctgg caataagaaa ttgtgcggat tttccgctcc tgaaggcaag ttctcaacga
     6721 aaaagctggc gtgtttgatc ttgccgatat catgataata gcaagctact cgagttagta
     6781 aagaacgtcc accgatttct gctacagcat tagcacttag attggctacc atcatgctat
     6841 gatgatacgt acctggtgct tcttctaaca actgtttcaa taaagggtga ttaggattgc
     6901 tgagttcatt taatacgatc acactatcat cattgaccat caattcaata taggggtgca
     6961 gacctatcgt cagcaagaat gagaacaatg cacctgtgaa tccgcagact agcgttaacc
     7021 aagtcgttgc atccccaaag ctcattcctt ggtagataat caaaatgaca tccatcatca
     7081 cagggaaaat aatgacccac atagcagcga ataacccttg ttctgagatc cgtttgcgtc
     7141 tgacgatcgt tgccatgaga ccagaaaata cataggacat caaaataact gtcagaaagt
     7201 tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa ataccagcca
     7261 tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggagcaaaa gccgcggggt
     7321 aaaacagcgg aatataagca gcttgttctg tttggaagag ttggaaaaat ttcattagta
     7381 ctacgctgac tgacattgct gtcacgtaga aaagtacgta ttttgttctt tctcctgcat
     7441 tttgaaattg aagtgaatta taaatcagta caccgatttg aagcaaaaca gctaaaacca
     7501 tggcaatgag tgggaaaatc gaagtcgttt gattcgtcaa tcctagtaat tccaattttt
     7561 tcatcgctga agcatcaatt tgatttcctt cgcgaacaat gatttctcct tggaagatca
     7621 tcactggttg tacagcttct cgtgcagatt gttttaattc ttctgtcttt ttctcattca
     7681 atacatcatt gactacaatg ccttgatcca ctaagtagcg gatcatttgt tgctgctctg
     7741 ttgtgacatt caaatactga atctgatctt ctgcttcttg tttaaaagct tccaattcgc
     7801 tttcacgaac ttgtttactc atctgttcgt caatgagctt caaactttca tccctaaccg
     7861 tgtgaatttc cgtttcagtc atttcaaata tcgtcgaata aaaattgttc ggcagttttt
     7921 ggtaaaacgc aacgttttct gcattcacgt tttcaaaatt tttcttcagt gtagcaatcc
     7981 gctcttctgt agtaggtttc gggatatttt catcttcctt cgccttatct accttgtcat
     8041 tataagattt gttgatggta tcattcgtct tatcgatcaa ttcgaataat tgcttgatcc
     8101 gattgttctg gtcatctgcc agatcttttt gataagtata ttctggagtc actgcttctg
     8161 ctgctagttt tcttctttgt tcggtttctt ccgtgttctc gatcgtttta ttcgcacgga
     8221 tactctcttc tgctacttgg ccttctctaa aatcattggc cttttgtttg acactactaa
     8281 atgtaacgcc gcaaacaatg ataaaaaaca agaacagaag gaaaggcaat gctgcctttc
     8341 cgagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc tcctaagcaa
     8401 agcttattct tgatgaagat ccgccttttc ataagctttg atgatttcgg caactacagg
     8461 gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg atttttttga tttgctttag
     8521 tgttcgttct gcatgaacaa gaccacttgt cgtccctcga ggcaagtcta tctgacttgt
     8581 atccccattg acaatcattt ttgattgata accgagacgg gtcaagaaca ttttcatctg
     8641 agcgactgtc gtgttttgtg cttcgtccaa gataacgaat gcatcatcca atgtccgccc
     8701 ccgcatgtaa gctaatggtg cgatttcgat tacgcctcgt tccatcaatc ggttcgtatg
     8761 atccaatcca aaaatttggt ataaagcatc ataaacaggc cgcagataag gatccacttt
     8821 ttctttcaga tcccctggta gaaagcctag gttttcccct gcctctacag cgggccgcgt
     8881 caaaatgatt ttttgcactt cgcctttttt taaggcagca attgctaata ctacggcaag
     8941 aaacgttttc cctgttccgg ctggccctac accaaatacg atgtcatgtt tggctaccga
     9001 ttcaacatat ttcttttgac cgctgttttt tacccgtata ggttttccat tacggtcttt
     9061 gacgatctct tcctcataca tctcaacaaa ataatctaat gtaccttttt gccccatttt
     9121 caatgccgtc acaacatcgg gcgtacttac gtggattcct cgtttgatca attcctgtaa
     9181 tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcaccaatga tctgaatgat
     9241 ttctccacgt gtatggatgg ttgtttccgt tgtttcctcg atcagtttta tatgcttgtc
     9301 gtgtgcacct aaaagcatgc tgacatcatc agcagctgtc aatctaatct ctaaagaact
     9361 agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc ctattttacc
     9421 atactcagaa gaaactatat agcaaataag aaaaggttgt aaaagagtcg tttaaccata
     9481 cgtaccaaga aacgttaaaa aatagccgtt tccattcgct aaaggtctcg ttttgataaa
     9541 aagaatctga gaatgtaagt tatatgaaag tggagaacaa aatattccgt ttcattcagc
     9601 aggtattctc taggtatttt tcttgtttca aaaaaagaaa acaaaatagt taaaatattc
     9661 ttgcatttca aaaaaaacag gtgtattatt acgtcaacaa ataaacatac agcaaagcgt
     9721 cgaggaaaac agtaggctat tcaacgattt attcccagtg agtcatgtaa gtgagaaatg
     9781 acaataaatc gaatagacga acatcatttc cgagtcaatg tcgaaaacgt agatcattgt
     9841 ggctgcctcc attatcaggc tagcggataa ctttatcaaa aagcgtccgt gaatgaagaa
     9901 gcttttttaa gcgaaaaaag gtggtaccgt tctagaatct agagcccttt tcccaagtgg
     9961 aaaagggctt tttttatatt ttaaggtggt ggttttcatg gaatctgata ttaatggcat
    10021 tactaattgc tgcaacgttt atatcgtcaa tcaaaatgat aattattgga ggaaaaattt
    10081 atggaagaaa caaaaactta ttcatggaaa caactattga tggtcagttc acttatcttc
    10141 gggatgttct tcgggtcagg taatctaatc tttcctgtcc acttaggtca aatggctggc
    10201 acaaactggg tcagtgctgg cgtaggattt gccatctcag gcgctctttt cccattacta
    10261 gctatcttag ctgtagtagt gacaaaaagt gatggtctgt atgacttagc taaacctgtc
    10321 ggaaaatggt atgctgcctt gttcttaatc cttgttcatt tgacgatcgg accatttttc
    10381 ggtacaccac ggacagctgc tactgctttt gaaatggctg ctaaaccatt tttgcctgaa
    10441 aaatataatt cattaggtat gtttttattc tctgctcttt tctttttact tgcttatttc
    10501 cttactgtcc atcccacacg gttggtcaaa tacgtcggaa agtatctaaa ctccttgttt
    10561 cttgcattat tagcaatcat ttttgtcgtt tctttattcc acccaatggg aaatctaaat
    10621 caatctgctg cagatactta ccaaacaaac gctttaacaa atggtgtcct agaaggttat
    10681 aacacggttg atgcagtagc tttgcttgcc ttaagtgtca cttttgtcca tgcagtccgt
    10741 gaccttggtt tcaaagataa aaaagtcaca gaattaacag caaaagctgg tagcctgagc
    10801 gttgcgctag aagtcatgat ctattttgga cttgttcttc tcggtgcgat gagtttgaat
    10861 aagatgtctt tatcagaaaa tggcggaact gcactttctc agattactaa tttttattta
    10921 ggtaacttcg gcgctagctt cttaggagtg atggtcacat taggtgtctt cactaccgct
    10981 atgggactcg ttgtttcttt tgcacaagat ttccataaac tgttccctaa agtcagttat
    11041 atgacatggc tgcggttaac gactttcgtc tcttttgtcg ttgcaaatgc tggtttggac
    11101 aatatcatcc aatggtcttt accagtattg atgcttttgt acccattgtc gcttgcgttg
    11161 atcttgcttt ccttaacagc aaaatttttc caaaagacac catttgtcta tcaagtaacg
    11221 atgttgtttg ctgctgttcc cgccgtattg gatatgctgg caaattcgcc tgctttagtg
    11281 agtcaacaaa gagttgtagc aagcatgctc gaattttatc atcatcacgt tccttttgct
    11341 gcattaggac ttggctggat ggtcccaacg ttacttggtt acgctggcag cttactcttc
    11401 tattacgcct atcgcctatc agggtacaaa caagaagcaa atgaactacc agaagaataa
    11461 ccagatcaaa cacaatttaa cataagaaat attaaacagg aaaaaaccag ctagatgaaa
    11521 gaatcctatt ctttcgtcta gctatttttt agatttcttt tttacgagca caaaaaaact
    11581 gcggacaatg attgtccgca gtttaaatga taaaattaag attgtaatag ttctttcaca
    11641 atggcattta cttggtttcc atctgctttt cctttgactt taggcatgat cgcacccatg
    11701 actttgccaa attctttcat tgatgaagca ccagtttgtg tgattgcttc ttgaacaagt
    11761 tgtcgaattt cttcgtcgga aagttgttcc ggcatgtatt tttcgactat cacaatttca
    11821 ctttttactt tttcagctaa atcgtcacgt cctgcttctt caaactcatg caaagaatca
    11881 cgtcgctgtt tcatttcgcg agacaacacc gttaattctt cttcttctgt caagtcgtga
    11941 ccttctttaa tctgctcgtt ttgtatagat gacttgatca ttcggatgac ttgtaaagat
    12001 tccttatctt tcgctttcat cgctgttttc atgtcatcgt ttaatctact tagtagtgac
    12061 acactaccaa ctccaattca tcacagaact agaatttttt gcgttttcta gctgcttcag
    12121 attttttctt gcgtcttaca cttggttttt cgtagaattc acgtttacga gattcttgta
    12181 aagtacccgc ttttgacacg gaacgtttga agcgacgaag agcatcgtca agtgattcgt
    12241 ttttgcgaac gactgttttt gacatgttaa ttccctccct ccgagcttaa aaagtaaccg
    12301 taattgttat tgaaatatat ctcaaaaaac aagactgtcc tgtaaacatt atatctaatg
    12361 ctgttttgac cgtcaaggaa atcttgcttt tttttaaaat tatcaaatgt tattttattt
    12421 aaatccttat tgacaatttt cacatcttcc gaaaatctcg aatctatgcc cttcgatcgt
    12481 acatccaccg agctgttctt cgaaataatt cattggacac atatggattt ctttcgttgt
    12541 tccgcacacc gtacagataa agtgatggtg atggccgatt tccccttgac agcaatgaaa
    12601 acgaaacttc atctctccat ttaaatcggt ttcttccagt aaatcgattt ctgcaaaatc
    12661 atggagattt cgataaatcg tatcgtaact tactcctttg aattcctgat tcatatagtc
    12721 aaaaacttct ttagcggata cgtagcgatt gcgcttcact aaataagtta acaatgcttc
    12781 tcttttttta gtatatttaa aaccgttatt tttcaatgtt tctagtgatt gttcgatcaa
    12841 tgtctgtttt ttcatgactt attcctccaa atcaaaatga ttacgaagta cacattctag
    12901 agtatggtat aataaacata aaaaaaatgc aagtgaggtt ttatccatgt cttccgattc
    12961 tacagaaatc gttacttttt ttgtcatctc cgactctgct ggagaaactg cgacaaaatt
    13021 agcgcaagcc actatggccc aatatcctag cgttgagttc aatttattcc gcagaacatt
    13081 tgtgacagat gagaaaacat tgatgcaggc actgcaagat gcactagaag aaaaagctct
    13141 tgtcttacac acattgatca atcaagaatt gatcgagatg acgcgttctt tttgtgaaga
    13201 acatcaatta tttagttttg atgtgatgac tccgccgata tcagaaatcg agcgcttaac
    13261 tggtatcaag cccattcggc aacctggcgc ccttcattta ttgaatgaga actatttcaa
    13321 acggattaaa gcaatggaat ttgctgtaaa atacgatgac gggaaagatc ctcgtgggtt
    13381 tctagaagca gatgtcgttt tgctaggcgt atcccgaact tccaaaacgc ctttgagctt
    13441 gtttttagct aacaaaaatt taaaagttgc caatcttcct ttgatcccac aagcacatat
    13501 tcccaaacag ctttgggaga ttgatcctaa aaaaatcgtg ggattgacga ataacccaga
    13561 tattttaaac aatatccgta aagaacggat gcgctcttac ggattaaatc ctgatactgc
    13621 ttattctgat atcgaaaaaa tacgagctga actcgatttt gcaaatgatc tttatgaaaa
    13681 actgggctgc gtagtaatca atgtcgcttc tttatctatt gaagaaacag cttcgcttat
    13741 tttgaatgct ttagacctag aagatcatag ttattatgga actgaaacca ccgaagaaac
    13801 agactaacag tttttcttgt ctttttctta aaaaaaactg gactgtgtca gcgaagaaga
    13861 ttcgttgaca cagtccagtt tgtctctatt tgcacctatc gttattgatg ttccacatgt
    13921 ttgagtgtct ggttcaaaat atccagtacg tgagaatcat caagactata caaaatcgct
    13981 tttccctcac gatgggactt gacgacatgg ttttctctta ataaacgcag ttgatgcgat
    14041 acagcagatt gttccatttc cactgcttgg gtgattgctg tcacattttg ttccccgtct
    14101 ttcagataca gcagaatttt gagacgagtg gggtcactca acattttgaa tagttggctg
    14161 actttctcta cttttgtttt ttgcgtgtct ggcaatccat tcacctccaa cttgaaaaaa
    14221 gactgacttc taaagtttac cactttttcc atcagtctca agttttttta ttcttctgtc
    14281 atcttttgga taatcgcttc ataagcggct actttttcag ctgcgtcttc tgaagagttt
    14341 ccttttacac cgatgtaaaa tttgattttc ggctcagttc ctgaaggacg gatagcgatc
    14401 cagccatttt cttccagcgt atacttcaat acatcagatg gcggtgtcgt tagtttttct
    14461 acgtttcctt cacggtctgt tctcgtcaac tctttgaagt cttctgtttg gatgactttt
    14521 gctccggcaa attctgttgg tgcttcttca cggaattttt ccatcaaagc agagattttc
    14581 gcagaacctt cttgtccgct cattgtcaca gaaatcgttt tttctgcgta gtagccatag
    14641 gtttcaaaaa tcgtttgcaa gccgtcatac aacgttttgc cttgtttttt atagtaagct
    14701 gctacttctg ctaataagag caatgcttgg atcgcgtctt tgtcgcgaac gaaaggttta
    14761 actaaataac cgtaactttc ttcgaatccg aacataaaag tatgggaatg atcttcttca
    14821 aattgttgaa ttttttcagc aatgaatttg aatcctgtca atacattgaa catggtagcc
    14881 ccatagtgtt ccgcaattgc tgtaggtaat tcgctagaaa cgatcgattt caataccgct
    14941 gcgttttcag gtaaagtgcc tcgcgattgg tgtgcttcca aaatatattg agcaaacagt
    15001 gctccgattt ggtttccggt caatacttca taggagccat ttggcaaacg aacggctgca
    15061 cctaaacggt ctgcatcagg atctgttgca atcagcaaat cagcgccttc tttctcgcca
    15121 agtcgtatcg catactcaaa agcagagtgt tcttctggat ttggtgattt gactgtagtg
    15181 aaatctgggt ctgcgacagc ttgttctgga actagtacga attgttcgaa tccagcttgt
    15241 ttcaacgcac gttcgcccaa catttttcct gttccgtgaa gtggtgtgta cacaagtttc
    15301 aaatctttcc ccattgtttc caccaattca tgatcaatcg ttactgcttt gatttcttct
    15361 agataagcgg catccacttc ttctccgatg atcgtgatca atccgctatg ttcagcttct
    15421 tcgtcagata atacttcaac ttttaacgga ttgctgactt cacggacata tttcgtcaat
    15481 gcatctgcgt ctgcaggagg catttgtccc ccatcttcgc cgtagacttt atatccgttg
    15541 taagctgctg gattgtggga tgcagttacc atgattcccg caaacgtctt caaatgacgg
    15601 actgcaaaag aaagctctgg tgttggacgt agactttcga atacataaga tgggatattg
    15661 tgatgtgcta atgttttggc tgcttccatg gcaaattctg gtgattgatg gcgtgagtca
    15721 taagcgatag cgacacctcg gcgttttgtt tcttcgcctt gtgtatccat gaaacgtgca
    15781 agtccttctg ttgcttgtct taccgtatag atgttcattc gattgatccc aggccctaag
    15841 attccccgca tgcctgcagt accgaattcc aacggtgcat aaaaagcatc ttcaagtttt
    15901 tcaggatctt cacccagatc atttaattgt ttttttaagt tttcttctaa attttcttca
    15961 tgtacccatt gttcatacac ttgttcccaa gacatgaaaa taacacctct ttctgaaata
    16021 tggttcttaa taagtatagc attcacttat tagatataaa agtatgagga ccaaaaaacg
    16081 cttaaatcaa gaaaggattc ttttatcaag cacggttgca agtattcgca taaatcggat
    16141 tcagtagatt tcacgcgaaa aaaaagagta aaagaacaat tgaaaccgtt cttccactct
    16201 ttttaaactt atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca
    16261 gcgatcccac catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct
    16321 ccgatggcaa aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagcca
    16381 gcttcgttcg tcaaccctgc ttctctaaat ggtgcagtta atggatccag tccaacatag
    16441 atgaatactc cgtctgctgc caaatcaaac tcattgccgg ttttgacatt acgtgctttc
    16501 acacctgtca ctaccatttc attcccaacg atttcgtcaa cgactgtatc ccacataaag
    16561 gaaatttttt cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct
    16621 ctacggtgga cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact
    16681 gctgagtctc ctcctccgat cactactaga cgtttgttac ggaaaaacgc accatcacaa
    16741 actgcacagt aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta
    16801 cggtggatac agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa
    16861 ataacttctt tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct
    16921 gtcccaaatc ttgaaacacc ttcatacatc ttataagcta gatcaggacc catgattgaa
    16981 tcgaaaccag gatagttttc gatttctgcc gtattgttca tttggccgcc aggggcgcct
    17041 tgttcgatca tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca
    17101 gcaggacctg cgccaataac gattacatca tacatatgta gattctcctt tacttaatca
    17161 agtaagcata ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga
    17221 atagctttat tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat
    17281 caatcattcg aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat
    17341 ttcttcttcg aacgggctaa tgtgccattt ccctcgttgt cacgatcgac ataaatgaat
    17401 ccgtaccttt tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc
    17461 gtatagccta atagatcgac tccatctaat tctactgcat ctttcattgc ttggatatgt
    17521 gctgccagat agtcgattcg ataatcatct tccacataac cattctcatc gggcgtatcg
    17581 actgcaccta agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca
    17641 ttcatcgtta ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga
    17701 tatggatttt tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca
    17761 gtagtcgaaa ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc
    17821 agcaatgcgg catccccagg ttccatttcg atctgaatgc catttcgttc catttctttc
    17881 aatgcataag cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga
    17941 tctgattttc tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct
    18001 gctgccagca tacagcctac tttgttctcc ggatctactt catgagcaat cttcgttgcg
    18061 atggcacttg caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct
    18121 ccttcttcaa aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca
    18181 ttgaatgtca accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga
    18241 ttttcataaa aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc
    18301 atcggacaat caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct
    18361 ttaaagacat cctcataaaa tttcaaccca gattcatttg gctctttttc atcgcccatt
    18421 ggaaaaatac gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat
    18481 aaagcaatat cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa
    18541 tgattttcat caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct
    18601 ccgattggca caacgtctac gttcgctaac ccacgtccgc cttcatcata accgccctcg
    18661 cattgattgg ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt
    18721 cctccattac atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc
    18781 cttctgcttt taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa
    18841 taaacaactc atccattatc cgttttgctg tctttcctga tcgtataagc ggattgatcg
    18901 taaacgcttg caaagctgtt ccataatcac cagtcacggc tgcttcgatc gtcagcaatt
    18961 ccatattctt cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta
    19021 catttcgtgc gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca
    19081 gatcaggaac agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat
    19141 agatcgaagc aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca
    19201 attgttcagg tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg
    19261 tttctttcac ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca
    19321 gcatttcttc ttcacggtag taatagcggt ggtagccaca aggaatcatg ttcatttgtt
    19381 ttaattgttc tttgaaaaac gggatatcaa agatattttt cggcaatcca ttgtcttcat
    19441 cgtataaacg atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt
    19501 gccaatggaa gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa
    19561 gcattgttgg ttctgtcatc atcgccatta ctggcacatt gcataatccg atcactttat
    19621 cccatttgcc atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattga
    19681 tcagccaagc atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa
    19741 cggtacgaaa agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat
    19801 aataactagg gatccgctca tctttgattc gtgcatctaa ttgaccgaca cgaaactgag
    19861 tcgtaacaaa atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga
    19921 cttcatacgg agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt
    19981 cttttccttc ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt
    20041 tgataaatcc ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt
    20101 tgattccttt tgccatgttc tcactccttt actttttaca cttttattat cttttttgta
    20161 aacaaaacaa acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt
    20221 cttctggatt tttgtaaata tcttgtcaaa aaatgtcttt attaagctat ataacataaa
    20281 tccgtttaca aatcattttc aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta
    20341 tctacatgtt attacgtgaa aaaatcaaag aaatctcctt ttctccttca gaaaatgaag
    20401 tgattcagtt tctcttaagc gattctccag cacttggcga aatgaccatc caagagattg
    20461 ctgagcgatg ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg
    20521 atggctgggt cagttttcgt aatgcttttt tagctgaaac agcctatctt gaagggaatt
    20581 ttcaagacgt agatgcgaat cttccttttt ccaccacaga cggtattatg acgatcgcaa
    20641 ataaaatggc ttctttagaa cagatgacga ttcaagatac actttctctc attcatcatg
    20701 accaactgca aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca
    20761 gtaactcgaa tactttgatt tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg
    20821 atgtcacgat tgtcaccact tttggtgaat cgttctatga agcgtacaat tgcacgagtg
    20881 atatgtgcgg gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag
    20941 aaatattgaa acaaaatcat gtgccaattt tagctttgac cagtatcggt gattcaacgc
    21001 tatcttcaat gagtacagcc gctttgcgac ttacgacacg cgaacgactg tattcgaaaa
    21061 ttggtaattt tacgatcaat acatcgattt gttatcttct agacgtgtta tatagttgtg
    21121 tttttgcaga aaattatcaa cagaatttag tccatctaat ttctgtagga caaaaatttg
    21181 ataaacgccc tatcagctca gccatcatga aagagccttt caattttgaa aataaagaag
    21241 tggactaaaa agaataatta agcctagtcc attctttcac ctaaccgttc aggtcagtgc
    21301 ggtgataagt gcatcgtctg gcccgaccgt ctccccttga ttttccaaaa tatccaaata
    21361 atccgtgtaa ttggtgacga tcacaggtgt ttctaaactg tatccttcct cttcgatttt
    21421 cttcttgtcg aagcggagca acagatcgcc ttgtttcact ctttctcctt gctgcaccaa
    21481 gcttttaaaa tattttcctt ctaactgaac agtatccaat ccgacatgaa tcagcaattc
    21541 cagacctgct tcagaaacca ttccgattgc atgtttcgtc gggaacaggg tcatgactgt
    21601 tccgtcaaaa ggggcacgta cttctccttt ttcagggtag atcaagattc ctcgacctaa
    21661 tgttccttgt gcaaaagctg catcctttgc tgcattcagt ggagcgattt gtccttgtat
    21721 cggtgttttt acaatttctt tacctgatgt tttctgtcta tcatctgttt catctgcttc
    21781 ctccttccaa aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat
    21841 caatacatta atcacatctg ttaaattacc atctggtcta atatagttgg gaattgtaaa
    21901 gatgcctagt ccaccttgat tccaagaagt taaattcatt aacccactat acgcgccccc
    21961 taacgcacca ccgacacagg taaaaataaa tattttttta cgtggcaatg taaacccata
    22021 aatagctggc tctgtaatcc ccacaatacc agaaacaatg gctggtattg caattgcttt
    22081 gtcttttgga tttctaagct taaagtagag tgcagtaagt gctgcagttt gtccaaaaga
    22141 cactactgca acaggtgtca tgatattttg ccagccattc acagaaaatt gcatcaatcc
    22201 cattggtacg attgcccaat ggagaccaaa catcattaga atttgccaga agaagcctac
    22261 gattgctcca taaataactg gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat
    22321 atcagttgcg aatgtgaatg ctggtccaac gagtaaaaaa cctaagggca ttgtaataag
    22381 aacggtgaag aatggaacga agaaattcgc gatcatctca ggtactaact ttttaattgg
    22441 tttctgaatt tggctagcta acaacacaat aaagataatg ggaatgacag aagaagcgta
    22501 gttacgcgca atccagggaa tgccgaatac tgtttgataa ataggtgctt caaaaatagt
    22561 gccagaaaat atggtcgtca gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa
    22621 gctaggattc attaatgctg ccccaagcaa catccctacg aattcattta ctttaaattt
    22681 acgggcagct gttaaaccga taataacagg cataaattgg aatacagaat caccgatatt
    22741 atcaaacata acaaatgtgc cagaaccgcg gtcaaatgcg cctaagaaga caagtaatgc
    22801 tgtcagtcca cgaatcatcc caccagcagc tagaatagca aggaatggct ggaaacagcc
    22861 cgatattcca tcaataagca tatcaaacgg tctcattttt tctgttgaaa cagcctcatc
    22921 aaaagtaagg tttcctgctc ttataacctc ttcaaacacg actggcacat gattcccgat
    22981 gacaacttgg tactgtcctc ccgctcgcat taccgtcaca acaccgtcca tatttttcaa
    23041 gatctcatca tttgcttttt cttcgttttt taatttgaaa cgaagccttg tgatacaatg
    23101 tgtcaaacca ttgatatttt ccttcccgcc gacattttct acaattttgc tcgctaattc
    23161 ttgatatttt cccatttcta ttccctccaa tttattagtg gaaggtttag ccaactgaat
    23221 gtcactaccc ttatttcatg cgttcttgtt aatttatcct ccttttttat ttatttacaa
    23281 cgattctcat gcactaaacg cgcagtatgg atcgttaaat acattttttc gtccgtggac
    23341 atctggtagt tgaatatctc ggctaaaaag tctgctattt tctcaacgca accaaacgta
    23401 tccttatatt ttttttgaat aacttggtac aattcctcag aaacttctcc cacttctgat
    23461 ttttctccat ctaggacgcg tgtcagaaaa aaacgtaggt gtgtgacgaa tcgaaaaaaa
    23521 tggactgaat cttcatcaaa tgtaatctta tagtaatagc ggacgatatt tagaatatcc
    23581 tgtattgttg tagtaaacag gtaggtattg tcttgttggc tttgaatgcc ttgtgcattg
    23641 actaaatgca atgcgataaa tccggcttca tcttctgata acttgatgtg gtatttatcc
    23701 gagattttct tcactgtttt ttccccgatg gcatactctt taggaaatag ttgtttgata
    23761 tcatacagca atacatttgg tacaaatatt cccattttgt gacgttcgat agccgtatac
    23821 aaatgatccg tcaatgcaat atacactgaa tcattcaact ggacttgtag agcctctttt
    23881 gtatattcaa tgatttcaca agaaatttct aagtactcaa gaggaatcgc ttgtgccaat
    23941 tcttggaact gttctgaaag tccgtcattt gtcaactgaa attttttgtc gatgcatgag
    24001 tcaaaaatcg ggtcacccac tttctttttg aatgccaaac cttttcccat cacgatcatt
    24061 tcctgatttc tttcatctct ggttattacg acgttgttat tcagtacttt ctcaatcagc
    24121 atgttttact cctttccatg atatataggg ctgtatccaa aacaaaaaga aaaacccaaa
    24181 aagccgtcca tggaaccatg ggagactcat caggtttagc ttgcatcagg caatcactat
    24241 ccaagttgta tcgttttctt tatattatcg aaagcaagcg cttttgtcaa cgctttctta
    24301 gtggctatta ggcacttctg actagctgtc gaatcttttt taaatctggt gactacgaca
    24361 ctaccgctca cagatgaaga atctgggaag ctctgtttta ttttcactct agcgcttagg
    24421 tcaaaatgct actatgctac tgtcccaact tctaatatac ggtgttccag tatcagctta
    24481 tcggtgataa gccaaattta tgcaaaaatc tggaaagcct tttttacaat ttttttctta
    24541 tcacctaaag ctgaacggag ccctgtcaca accacttttt acacaccact gctttgcagt
    24601 ttgtacatat ctgcatacaa accattttgc gctagaagtg tttcatgatt cccgcgttct
    24661 actatatgac ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga
    24721 tgggcaatcg caatcgttgt ccgtccttgt cgcatttttg tcaatccttc ttggatcaat
    24781 ccttctgttt cagtatcgat atttgctgtc gcttcatcca atactagtat tttaggatct
    24841 gtcacgattg ttcgcgcaaa agtgatcagc tgtcgttgtc cgctagaata gctggcaccg
    24901 tgttcgatca cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga
    24961 acaaactcag ctgcatggcg aatctgttcg tctgtgatag ttggattcat catccggata
    25021 tttcctgcga tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta
    25081 cgtaactctt ccattggata ttctcgaatg tcatgatcat caatcaagat ctggccttca
    25141 taaaattcgt aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc
    25201 cccactaatg caacggtctc tcccggatta gcgacaaaag agatattgtt caatacattg
    25261 tgctctccat cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgtt
    25321 tgatctgctc caggattttg ttgcggtgca tatttttctg tatccatgat tttcaaaata
    25381 cgactaccgg caaccattcc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt
    25441 ggattaaaaa atccctggac ataggtcgta aatgcataga tcagtccaac ttctacggga
    25501 gaatgcagcg catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatataga
    25561 aaattgatga tcggacttaa cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct
    25621 aaatactcgt tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc
    25681 tggataatac gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt
    25741 tcccgcatat tccggtaaat acgagaactg aatttctggt aataccagat gacgattcct
    25801 aaaatcggca agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca
    25861 atgaaggaag aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat
    25921 tcaaatagcg tttctgtgtc atttgtcact cgagaaacgg tcgatcctgc cggtgtctga
    25981 tcaaaataac gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga
    26041 taggtcttca atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg
    26101 actatgactc caaacaagta cataccagca aaaaacaaaa taacttgtgt cgtagccgac
    26161 tgtttagcca gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc
    26221 acagacagag caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg
    26281 aacttcatca gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat
    26341 tccgattgat aattttcatc catcaggcct cgcctccttc tatcttcgct tctaattgct
    26401 gtttctccca cattcgttca taccagccgc ctaatgcaag cagctcatga tgggttcctc
    26461 gttcaatgat tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta
    26521 aacggtgagc tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga
    26581 tagcttcttc tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag
    26641 gatttttgat caatgctcga gcaatcgaaa tcctttgttt ctgtccgcca gataaagaga
    26701 ctccacgttc tccaactaag gtatcataac cttttggcaa ttccttgatt tcattgttga
    26761 taaatgctaa agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac
    26821 ggatattatc tcgaatggtc atcgaaaaca agaagtgatc ttgaggcaca tagccgattg
    26881 atcctaataa agcattcaat gaatattctt tgatcgaacg tccgccaaat tggatttttc
    26941 cttgataatc atcgtattca cgcatcagta atttgatgat cgtcgttttt cccgcaccag
    27001 ttttcccaac aatacctaat gtttctcctt cttggatcac aaatttgatt tgttctaatg
    27061 ctggtgattc ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg
    27121 ccggggtctg cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat
    27181 tgacgcgatc ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa
    27241 acatcggcca aacaagcata ccgatatagc taataaatga gaccatctgc ccgatcgaga
    27301 tcgtgccttc caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga
    27361 taatcaaggt aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa
    27421 taattgcatc ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg
    27481 ttttaatgac tttgattcca gtaatacttt cttgtgtctt atcattgata ctagaaaaag
    27541 cttcttgcga atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg
    27601 ctagtaaagg cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca
    27661 tcgcaatgat cgttgttccg cccgtgatca ctgaatctgc aaaggtcaag ataccagctc
    27721 ctgctacatt ttggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc
    27781 gatatttttg gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa
    27841 gttctttctc caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa
    27901 gatattgtaa gagagctgca aggatcaata ataagatcca ccaggaaatg atctttattg
    27961 ataaattttt atcggcaatc tcatcaatga tgatcccaat cacctttgga ggaactaact
    28021 gtaccaaggc cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgtc
    28081 gaaaaaacca acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag
    28141 agcataggat tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta
    28201 cgaaataaaa aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt
    28261 ttttcccttg gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt
    28321 gccccattga catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta
    28381 aatagtcttt catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag
    28441 caataatagc aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt
    28501 tccgaattgc tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc
    28561 tttagtttca tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac
    28621 cagcaagaca cgccacgaaa aaaaattagg cataccgaat atttttcttt aattttttct
    28681 ttctgaggtg tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc
    28741 aatccaacaa acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag
    28801 ctgaagtaaa tggaacagtg gatgtaccca agggaaaaag tttttttaaa acacttcttg
    28861 cttactctgg accaggtgca ttagtagcag tgggttatat ggatccaggt aactggtcca
    28921 cttcgatcac tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta
    28981 gtctgattgc tatgctttta caatatatgg cagcaaaatt aggaatcgtg acccaaatgg
    29041 atttggccca agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct
    29101 tgactgaatt agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc
    29161 tctacctgct ttttgatatt ccattagtga ttgccgtatt tatcactgtg ttagatgttt
    29221 tcgtcctctt attgctgaca aaagtcgggt ttcgtaaaat cgaagctttg gttgtctgct
    29281 tgattttcgt cattttattc gttttcgtct atcaagttgg cttgtctaac cctgattggc
    29341 acagtttgtt tgccggcttt atccctaaca gtcatacatt ctcagactca ccggtcatcg
    29401 gcggtgaaac tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata
    29461 acttatatct tcattcagca gtttctcaaa ctcggaagat cgatcaccaa gatgaagaag
    29521 atgtagcaca agcggtacgc ttttctactt gggattctaa tatccagttg tcaatggctt
    29581 ttctagtcaa tgcactgcta ttgatcatgg gtgtcgctgt attcaaaacc ggttctgtcc
    29641 aagatccttc cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg
    29701 gtatcttgat tgctgtcgca aaatctggta tcttgtccac tttatttgct gtagcacttc
    29761 ttgcttctgg ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag
    29821 gcttcatcca tatgcgtatc ccgatttggc taagaagact agtcacacga ttgctttctg
    29881 tgattcctgt attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacata
    29941 ccgctttgaa taacttgatg aatgaatccc aagtttttct ggcttttgca ttgccattct
    30001 cgatgattcc tttactgatg atgaccaata gccaagcgga gatggggagc cgattcaaaa
    30061 attcatttgt tgtaaaacta cttggctggt tctctgtgat tagtctgact tacttgaact
    30121 tacgaggatt accaggtcaa atcgaaagtt tcttcggcga gcaagccacc tcagctcaac
    30181 tagcattggc tgatcaaatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat
    30241 ggacgattgg tgatttatat aaaggaaaca aaaaatatga acagcgtttg caagaaacga
    30301 tagaagaaat cgaatagaaa ctgattcttt tacacaaagg aggttgtgac agaagtgggc
    30361 agcttcaaga aataagaagg aattta
//
LOCUS       DACEJZ010000027        30362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEJZ010000027 DACEJZ010000000
VERSION     DACEJZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.027"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            372..1061
                     /locus_tag="IW656_001112"
     CDS             372..1061
                     /locus_tag="IW656_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836831.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1048..2256
                     /locus_tag="IW656_001113"
     CDS             1048..2256
                     /locus_tag="IW656_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0836832.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2550..3821
                     /gene="serS"
                     /locus_tag="IW656_001114"
     CDS             2550..3821
                     /gene="serS"
                     /locus_tag="IW656_001114"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0836833.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4066..4761)
                     /locus_tag="IW656_001115"
     CDS             complement(4066..4761)
                     /locus_tag="IW656_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0836834.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(4851..5951)
                     /gene="ychF"
                     /locus_tag="IW656_001116"
     CDS             complement(4851..5951)
                     /gene="ychF"
                     /locus_tag="IW656_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0836835.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(5960..6151)
                     /locus_tag="IW656_001117"
     CDS             complement(5960..6151)
                     /locus_tag="IW656_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0836836.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6172..7062)
                     /locus_tag="IW656_001118"
     CDS             complement(6172..7062)
                     /locus_tag="IW656_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0836837.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7049..7816)
                     /locus_tag="IW656_001119"
     CDS             complement(7049..7816)
                     /locus_tag="IW656_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0836838.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(7828..8541)
                     /gene="rsmG"
                     /locus_tag="IW656_001120"
     CDS             complement(7828..8541)
                     /gene="rsmG"
                     /locus_tag="IW656_001120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0836839.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8773..10674)
                     /gene="mnmG"
                     /locus_tag="IW656_001121"
     CDS             complement(8773..10674)
                     /gene="mnmG"
                     /locus_tag="IW656_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0836840.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10691..12088)
                     /gene="mnmE"
                     /locus_tag="IW656_001122"
     CDS             complement(10691..12088)
                     /gene="mnmE"
                     /locus_tag="IW656_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0836841.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12432..12770
                     /locus_tag="IW656_001123"
     CDS             12432..12770
                     /locus_tag="IW656_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836842.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(12938..13696)
                     /locus_tag="IW656_001124"
     CDS             complement(12938..13696)
                     /locus_tag="IW656_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0836843.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13714..14532)
                     /locus_tag="IW656_001125"
     CDS             complement(13714..14532)
                     /locus_tag="IW656_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0836844.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14529..14885)
                     /gene="rnpA"
                     /locus_tag="IW656_001126"
     CDS             complement(14529..14885)
                     /gene="rnpA"
                     /locus_tag="IW656_001126"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0836845.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(15003..15137)
                     /gene="rpmH"
                     /locus_tag="IW656_001127"
     CDS             complement(15003..15137)
                     /gene="rpmH"
                     /locus_tag="IW656_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0836846.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15660..16994
                     /gene="dnaA"
                     /locus_tag="IW656_001128"
     CDS             15660..16994
                     /gene="dnaA"
                     /locus_tag="IW656_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0836847.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17192..18322
                     /gene="dnaN"
                     /locus_tag="IW656_001129"
     CDS             17192..18322
                     /gene="dnaN"
                     /locus_tag="IW656_001129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0836848.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18551..18793
                     /gene="yaaA"
                     /locus_tag="IW656_001130"
     CDS             18551..18793
                     /gene="yaaA"
                     /locus_tag="IW656_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0836849.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18780..19904
                     /gene="recF"
                     /locus_tag="IW656_001131"
     CDS             18780..19904
                     /gene="recF"
                     /locus_tag="IW656_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0836850.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            19901..21847
                     /gene="gyrB"
                     /locus_tag="IW656_001132"
     CDS             19901..21847
                     /gene="gyrB"
                     /locus_tag="IW656_001132"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0836851.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            21867..24338
                     /gene="gyrA"
                     /locus_tag="IW656_001133"
     CDS             21867..24338
                     /gene="gyrA"
                     /locus_tag="IW656_001133"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0836852.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24574..24873
                     /locus_tag="IW656_001134"
     CDS             24574..24873
                     /locus_tag="IW656_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0836853.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            24923..25435
                     /gene="ssb"
                     /locus_tag="IW656_001135"
     CDS             24923..25435
                     /gene="ssb"
                     /locus_tag="IW656_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0836854.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25461..25697
                     /gene="rpsR"
                     /locus_tag="IW656_001136"
     CDS             25461..25697
                     /gene="rpsR"
                     /locus_tag="IW656_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0836855.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            25840..27813
                     /locus_tag="IW656_001137"
     CDS             25840..27813
                     /locus_tag="IW656_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0836856.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            27819..28271
                     /gene="rplI"
                     /locus_tag="IW656_001138"
     CDS             27819..28271
                     /gene="rplI"
                     /locus_tag="IW656_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0836857.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28545..29912
                     /gene="dnaB"
                     /locus_tag="IW656_001139"
     CDS             28545..29912
                     /gene="dnaB"
                     /locus_tag="IW656_001139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0836858.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         9310 a         5893 c         6026 g         9133 t
ORIGIN      
        1 tcagttatct tatcatgtca tttccaacat gtcaacaact tattttttat tatttaataa
       61 gttattggtg ttcttttgaa caaataataa tatatcaaca tcacatgtga aagtcaataa
      121 gaaaatgctt atttatcaac atttctagct tacatgatca ttggttagtt ctggtccttt
      181 ttcgttcgtc tttataactc cgagagacgt aaaatccttt tgttttcagt ttaatgttca
      241 tgttcttgtc ttattcacac caactagatg tctcaccttc taaaattttc cttaacaaag
      301 tgctgctttt atctatttct gctttttttt gctagaataa tagtaacaat atgactctgg
      361 aggatttatt tatgaaaatt ttgattgctg acgatgataa agaaatcgtt gaattattaa
      421 gtatctatgt tcacaatgag gggtatgaag cagtaaaggc ttatgatggg aaagaagcat
      481 tatctaaaat cagaacaatc cctgatattg aattacttat tttagatatc atgatgcctg
      541 agatggatgg gatgcaagta gtgaaagagc tacgaaaaga atcccaaata ccaatcatta
      601 tgctgacagc aaaaactact gatatggata agatcaaagg actggtcgct ggtgcagatg
      661 attatgtgac caagccattt aatccgttag aagtaatggc tagagtaaag tctttattac
      721 ggcgtacaaa aatgcagatt gccccagatc agcctgatgt attagaagta aatgcattaa
      781 tcatcaataa agactctcat gaagttaaaa caatcgatgg gaaagaaatc cagttaacag
      841 ctttggaatt cggtatcctc tacttgcttg ctagtcatcc taatcgagta tttagcgcgg
      901 atgaaatttt tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa actgtcatgg
      961 ttcacgttag tcatttaaga gataagattg aagaagcaac tggtggtgaa aaagtcattc
     1021 aaaccgtttg gggagtgggc tataaaattg acgcaagata gtaaacgagc aactgaaaaa
     1081 cgccgtcgga tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc
     1141 acgattatcc ttttgcttct attaaatgtt tctattctgg ttgttatcag ttcggtgatc
     1201 aacagttcac cttctttgac gaatgcgatt tgggattcaa agaatatttt tgcagaacga
     1261 ttgaacactg atttgttctg gaacggccgg aattttatta ttcccttttt ctttctatta
     1321 gatattggtg ttttgtattg gcgcttgatt cgaagatatc gccaaatgca attgcggcat
     1381 atcatcagcg aacttcacta catcgcaaac ggaaattatg atcatcgtat cccttttgaa
     1441 ctgagtggtg acttaagccg agtggtaaca agtatcaatg gattggtgga tagtaccgta
     1501 gcagcgattg aagacgaacg taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc
     1561 agtcatgata tccgtacccc actcacgtcc atcattggat acctaggact gatagaagat
     1621 ggccagtatc actcagaaga agatctttta aagtatacgc atactgctta tatcaaagca
     1681 aagcagatga aatctttagt ggacgactta tttgaataca ctaaggtacg ccagcctgct
     1741 gtcccggtga atttttctgc ctttgacatg atccagttga tcgaacagtt agcagctgat
     1801 tttgagttgg aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa agtagactcc
     1861 cttatcatgg atggcgatac cgaaaaactt gtacgtgtct tcaataatct tttgacgaat
     1921 gcgttgaaat acggaaaagg agcaacgaag atcgtaatcg aagtagaacg tatcggttca
     1981 gaagttgttg caacagtcaa aaataacgga gcaatgatcc cacaacaggc aatcgataat
     2041 ctattcgacc gtttttatcg tgtagaagaa tctcgttcac aagcaactgg aggaactgga
     2101 cttggtttag caatcgctca aagcattgtg gctttgcacg gcggatacat ttatgcaaaa
     2161 tcagataagc aatggacttc tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta
     2221 cctattgata cacaggaagt tattgacgaa agctgaaata ttcgctattc ttattacaat
     2281 tattagaata aatgaaaaac acagaaccca gtacacttta ttctttttca gagagctgat
     2341 ggcaggtgta aatcagcaaa ggataatttg gaagttccag ttcgtttacg tgccctttcg
     2401 aaataagaaa ggtcggttca acagccgtta tgtgttcaac aagtgcagct gacgctgaac
     2461 ttgggtggta ccgcgaattt cagactgatt cgtcccaaga ataatgggat ggatcagtct
     2521 ttttttattt tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg cgacaaaata
     2581 ttgaagaagt acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat
     2641 tcatggaact agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat
     2701 atcgaaatga tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg
     2761 ctaaaatcgc tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat actgaaattg
     2821 ctgaagtgga tgaaaaattg aaacaaattg caactacttt gccaaacctt ccccatgatt
     2881 ctgtgccagt tggaacagat gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac
     2941 gtacatttgc ttttgaacca aaaccacact gggaaattgc agaaaatctt gatattttag
     3001 attttgaacg tggcgcaaaa gtagccggta gccgttttgt ctattacaaa ggattaggtg
     3061 ctcgattaga acgtgctgtc tacaacttca tgcttgatca gcatatttac gaacacggct
     3121 atactgaaat gatcactcct tatatcgtca acagtaaagc gatgtttggt acgggacaat
     3181 ttccaaaatt caaagaagat gttttccaat tggagaatac tgatctaaca ttaatcccaa
     3241 cagctgaagt accattaact aactattata atgatgaaat tttagatggt gcagatttac
     3301 ctatttattt cactgcacta agccctgctt ttcgttctga agccggaagt gctggtcggg
     3361 atacacgagg actgattcgt cttcaccaat tcaataaagt agaaatggtc aaattttcgg
     3421 atgtagagca ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa gatatcttga
     3481 aaaaattgca tttgccttat cgtgtgatgg ccttatcaac tggagacatg ggcttttctg
     3541 cagctaaaac ttatgactta gaagtatgga tacctgcaca agatacttat cgtgaaatca
     3601 gttcttgttc taactgtgaa gatttccaag cacgccgcgc aatgatccgt tatcgcgata
     3661 atgatggaaa aatccactat actcatactc tgaatggttc aggacttgca gttggccgta
     3721 cagtagctgc cattttggaa aactaccaaa atgaagatgg ttctgtcaca gttccagaag
     3781 cgctcgttcc ttatatgggg aatctgaaag ttattaaata aagaggctgt gaaaaagccg
     3841 cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
     3901 tggacttaat accgagaaac tggcttttga acaccgttta tcgtaaaaag ggactgtgac
     3961 aagacttttg tcacagtccc ttactattat ttaactatgg ataaacaaaa tgaggttctt
     4021 tactacccct agatatctag caaaatagct gatctttttg ctagtttatc ttcttgggac
     4081 ggataaactt ccaataatcc cgtatttttt cttcaagtag taacggattg ctaaagcagt
     4141 tgccccgata atgataacta ctactggatc taatactgga ttgataactg gtggcaatag
     4201 tgttgtcgca gaaaacagta cgacccaaag aagtgtcgtt ccacctagga taagcaatgt
     4261 cttacccatt cctggtttct tgcttcgatc tgctccggga tattcatatt gatagatgta
     4321 tttgtacatc atataaaaga cccagccacc gattgcagaa cctagaacga gtgtagaaaa
     4381 gccataagct ggcgttgttc cacgtgaaaa caatccggta atcgcaatca tgatacttaa
     4441 tagaccaagc aacagcaacg tattatctaa ccacatcaat aaaggggtgg atgaattttt
     4501 tacttctgct ggcttattga tgattgcttc cgttctttca gagactgtcc caaacaattg
     4561 acgggctgtt cttccgcctt tttgttctgc aactaaagta ggcagaatgt catgaagggc
     4621 ttttactttt tcttcttcag acaaattcgc tgcttctaaa gattttttca aatcaaaaat
     4681 gtactgttga tttcttttgg ttaattttgt ttctaaacta ttgttttctt ttacgattgc
     4741 tcgtaattgt tctggttcca ttatctaata gtcctcctta gccgttcctt ttctcaacgg
     4801 tcgtcacaaa agacattcta ccatattcta cagctagttt gatagccctt ttacacatta
     4861 aagcggaata acattacgtc gccgtcttgt acgacataat cttttccttc taatcgaaca
     4921 cgtccagctt cttttgctgc atgcatgttt ccgtaatgat ccaagtcttc aaaagagaca
     4981 gtttctgcac ggataaatcc gcgttcgaaa tctgaatgga tgattcctgc tgcttgtggt
     5041 gctttgattc cttttttgaa tgtccaagca cgtacttctt gttccccagc agtaaagtaa
     5101 gtagctaagc ctaacaaatc ataagcagca cggatcaact gatccaaacc tgattcttcg
     5161 attcctaaag cttctaaaaa ttccgctttg tcttcttcat ctaattctgc gatttcttct
     5221 tctgcacgag cactgaccac gatcacttct gcattttctt ctgcagcaaa gttgcgcact
     5281 tcttgtacat aatgattcgc gtctgcatct gctacgtcat cttctgagac attggctaca
     5341 taaagaattg gttttgtcgt caataaaaat agtgatttaa cgattttttg ttcttcttca
     5401 gtaaattcga tcgttcgggc agattttcct tcttccagta caggtttgat tttatccagt
     5461 acagctagtt ctgccactgc atctttatct ttggttttag caacttttgc tacacgagta
     5521 tagcgtttgt tgatagattc taagtctgct aacactagtt ccaagttgat cgtatcaata
     5581 tcttctagtg gatcaacacg tccttctacg tgagtaatat tatcatcatc aaaacagcga
     5641 acaacgtgac aaatagcgtc tacttggcgg atatggctta agaattgatt tcccaaacct
     5701 tctcctttac ttgcgccttt tacgattcca gcaatatctg taaattcaaa agtcgtcgga
     5761 acagtttttt tcggatgtac aagttctgtt aagcgttgca aacgccagtc aggtacttct
     5821 accatcccta cgttggggtc gatcgtcgca aagggatagt ttgctgcttc tgctcctgct
     5881 tttgtgatcg cattaaacaa agttgatttt cctacgttgg gcaacccaac aattccagca
     5941 gtcaatgcca taaatttatt cactctttct ctgcattttt ttcaattact ttttttaatt
     6001 tcttttcaaa atctcgacgg gtcatcatga cgatatgtcc gcaattcata catctgattt
     6061 tgatatctgc gcccattcga atgacttccc agcgattcgt ctggcaagcg tgaggttttt
     6121 tcatttcaac gatatcgtgc aagtcgtaca taaaaatagc ctcctttttg tttattcttc
     6181 atcaaattga atattcaaga tatcaagaat tcgagtcaaa tctttttgag aaagatattc
     6241 aatctcgatc ttacctttcc catttttttc ttggatagct acacttgtac caaatttatc
     6301 catcaaacga tcttcgcttt ctcggatata atatggtttt tcctttgcta gccttgggat
     6361 ttttttcttt tcttttccat cattttgatt taactcagaa acaagctgtt ccagttggcg
     6421 gacagttaaa ttttcagtta ctgcacgatt ggctaatttc aagatcaatg ttttattttt
     6481 taaacctaac agcgtacggg cttgtcccat agataaacgc tgatcttgta ccatttcctt
     6541 gacaagatcc ggtaaagaaa gcaagcgcaa ataattggcg atataaggtc tgctctttcc
     6601 taaacgacta gctacttctg cttgtgttag tttcaggttt ttcatcagca tctcatatgc
     6661 ttctgcttct tctaacggat tcagatcttc tcgctgcaag ttttccagca ctgctacttg
     6721 catcatcgct tcttcgtcaa agtctctaac gatagcaggg atcgtttctt tttcagctag
     6781 tttggacgct cgaaaacgtc gctcccctgc aataatctca tatcctttta ctgcggaacg
     6841 gcgtacgatg atgggttgaa agacacctga ttgttcaata gaattagcta gttcctgcaa
     6901 agatacttct tcaaatgttt tacggggctg atacggattt ggtcgcagct cattcaatgg
     6961 tagctggaca acggtatcat cttttacatc caccgtctct aagttttcta aatcttgaaa
     7021 cagtgcatcg attcctcggc ctaatccttt acttttcttc acgacttacc acttcctttg
     7081 ccaatgcttg gtagacttca gctcctcgag aacgtggatc gtagtcgatg atcggttttc
     7141 catgactagg tgcttctgat aaacgaatat ttcttggaat gatcgtttcg taaacttttt
     7201 cgcggaagta tttacgtact tcttctacga cttcatttcc cagatttgtt cgtgcatcat
     7261 acattgtcag taagactccc tcgatctcta attctggatt aaagtgtttt tggactaaac
     7321 gaaccgtatt cagtaattgg cttaatcctt ccaatgcgta atactcacac tgaacaggta
     7381 tcaaaatcga atcacttgct gtaaatgaat tgattgtcag atggccaaga gaaggcgggc
     7441 aatcgatcaa aatataatca tactgactgc ttacttctgc taaagatcct tttaatcttg
     7501 attctcttgc catcattgac gtcaattcga tctctgctcc tgctaattgg agagtcgcag
     7561 gcacaatact caaattttcg tgttcagtga tcaaagttgc ctcatcaatt ggcaattcat
     7621 tgactagaac atcatagatg tctcttgtca catcaggttt tctgatcccg acaccactgg
     7681 tagcatttcc ttgtgcatcc atatccacta gcaatacttt tttgccaagg ctagcaagac
     7741 aagcacctag attcaccgtc gtcgttgttt tgccgacgcc acctttttgg ttcgcaacag
     7801 atattatttg tgccatctct tctcctccta tttgattggc tgcttattag gcagtccggg
     7861 ctttcttggg tatttctttg gggttggttt ctttttttga ataacgatga tatgccgttg
     7921 atcagagaca ttcggcaagg taaccgcctc ttctttgagg aatttaccac ctaataaagc
     7981 aatcgcttgt tttgcttctg tcaattcctc ttcacttttt gctgctttca atgcataaaa
     8041 atagccttct tgtttagcca agggcaaaca caattccgct aacacattca aacgagcaac
     8101 cgcacgagca gtgacaaggt caaatgatgc acgaaagtga ggattttgcc caaatgtttc
     8161 tgcccggtca tgatataagg aagcatcgat tcctaataat cgggttaact cagataagaa
     8221 ggttattcgt ttattcagag aatcaacgat cgttatctgc agttctggaa aggcaatttt
     8281 taaaggtatg ctaggaaacc ctgcccccga accaacatcg cataaagata aaggttgcgt
     8341 aaagtctact tcaaatgcta atgtaagtga atcgtaaaaa tgcttcagat atacgtcttt
     8401 ttcatccgta atagctgtaa ggttcatctt ttcgttccat tcgactaatg tccgataata
     8461 aatagcgaat tgattcattt gatgatcggt aagctctatc cctttttctg ccaaggattg
     8521 gcgaaattct tctggtatca taaatattcc tttcattgtg aaacagcaat tgtttcacag
     8581 attctctccc tactttaacg caaaatctct agacatgcaa tccttccata tggattctta
     8641 aaaatttgaa aagtaagaaa agccgataga ctttttcttt ctagtatgac tcataaaata
     8701 tacacagaag agagaactag ctttgagcaa tttctgcaaa aactagttct ctcttctatt
     8761 tattattacg tattacgctg gttcactgac tttcgcaatc ttcccttgtt cgatatagac
     8821 cattaggata cttaggtctg cagggtttac gccactgatt cggctagctt gggcaattgt
     8881 ttctggttgg attttctgca atttttgttt tgcttctgtt gctaatccgt tgattgctgc
     8941 gtagtcgatg ttttctggaa tctttttcgc ttccatgcgt tttaattttt ctactttttc
     9001 catggctttt ttgatgtagc cttcatattt gatttggatt tctacttgtt cgatttcttt
     9061 tcgtgtcaat tcttcatttt ctggaataaa ccgtagaagt tctgcataag taatctcagg
     9121 acgtcttaaa aagtcgcttg ctagtacacc atcttttaat ggtgccgcat taatcgttgc
     9181 taaaaagtct tggacttctt ctgttggttt gattcgtaca ttcatcaaac gtttgatttc
     9241 ttgttcaact gcttcttttt tcttcaaata attcttgtat tgatcttctt ttactaagcc
     9301 tacacggtga cccatttctg ttaatcgcaa atctgcatta tcatgacgta aaagcagacg
     9361 gtattcagca cgtgaagtca gcaaacgata aggttcgttc gttccttttg ttaccaaatc
     9421 atcgatcatg acaccgatgt aaccgtcact tcgtttgatc actaaaggtt cttttccctg
     9481 aactttcaaa gctgcattga taccggcaat cagcccttgt ccagccgctt cttcataacc
     9541 gcttgtacca tttgtttgtc cagctgtata aagattttca acgactttag tttctaatgt
     9601 cggacggagc tggtgtggct ctactacgtc atattcgata gcatatcctg tacgcatcat
     9661 ttctactttt tctaatccag caatagaatg aagcatgttg acttgaacat cctctggtaa
     9721 agaagtagat aaaccttgaa catagacttc ttccgtattt aagccttctg gttccaaaaa
     9781 taattgatga cgtggtttgt ctgcaaaacg cacgatctta tcttcgatgg atggacaata
     9841 gcgagcgcct accccttcaa cgatccctgt aaacattggg gcacgatgta agttgtctcg
     9901 aataatttgg tgagtcgtct cacctgtata ggtcagccag caagattcct gatctagttt
     9961 ataggcacta tctggtgtgc tgaagctaaa atgattcggc tctttgtcac caggctgctc
    10021 ttctgtaaca gaatagtcga tcgtacttga ttttactcga ggcggtgttc ctgttttgaa
    10081 ccgttcgata gcaaagccaa gttcttttag atgattcgct agtccaatag aaggttgaga
    10141 gttattcgga cctgatgaat atttcaattc accgataatg atttctccgc gaagtgctgt
    10201 tccggctgta ataacaactg ctttggcccg gtagctcgct cctgtggacg tcactactcc
    10261 atgacaaaca ccatcttcga cgatcagttc ttcgacgatt ccttgtctta gcgtcaaatt
    10321 tggttctttt tcgatcgtat gtttcatttc tgttgaataa gcatgtttgt ctgcttgtgc
    10381 tcgcaatgct cggacagctg ggccttttcc tgtgttcaac atacgcattt ggatataggt
    10441 tttatcgata ttcttaccca tttctccgcc taacgcatcg atttctcgaa cgacaacgcc
    10501 tttagctggt ccgcctacag aaggattaca tggcatgaat gctaccatgt ccaaatttat
    10561 tgtcagtaat aatgtttttg atcccatacg tgcagcagca agtgcagctt ctgaaccggc
    10621 gtgtcctgcg ccaacaacga tcacgtcata cgcatctgct tcataatgtt ccaatcgttt
    10681 gttcctcctt ttattttcct aaacaaaatt gactgaataa ttgagtgatt agctcatctt
    10741 gtacactgtc gccgacaact tctcctagat agtcccagca acgcgtcata tctatttgaa
    10801 cgagatccac aggcattcct gaatcaatac ctgcaatgac ttcttcaagt gagagggaag
    10861 ctttttctaa caacgcgata tggcgggtat tagaaacata agtagcgtct ctttctcctg
    10921 tttctccgct gaagaataaa tcagaaatag ctgattccaa acgatctagt ccgtcgtttt
    10981 tagctacgga aattgaaaaa actggttcat tttcgatcaa tttcttcaat ttttcttgtt
    11041 ccagctgtgc tggcaaatct gttttattta gaagaatgat ccgtttcaat ccacttgtcg
    11101 cttccaataa ttgttcatct tctgctgtca gtggttcgct ttggtttaga acaagtaaga
    11161 tcaagtctgc ttctgctaat gcttttcggc ttctttcgac cccgatccgt tccaccacat
    11221 cttctgtttc ccgaattcca gctgtatcga ttagcttgag cggtaccccc cgaacattga
    11281 catactcttc gattacatct cgagtcgttc ctgcaatatc cgtaacgatt gctttttctt
    11341 ctcttaacaa atggtttaac aaacttgatt tgccgacatt tggccggccg atgattgccg
    11401 tacttaaccc ttcccgcaga accttccctt gcttggatgt agcaagtagc gcttggattc
    11461 tttgttggat catctgcgct ttttcaagca ataacttcgt agtcaattct tcgacatcat
    11521 catattctgg ataatcaata tttacttcta cttgtgctaa tgtctctaaa atctcttgtc
    11581 tcaatgagcg gatcaaagca gacagattgc cgtctaactg attaagagct agtcccattg
    11641 ctttgtctgt ttttgcccga atcaaatcca tcacagcttc tgcctgtgaa aggtcaacac
    11701 gaccattcaa gaatgctcgt tttgtgaatt cgcctggctc agctaatctt gcaccttctc
    11761 ttaacaatag ctgcaaaatt tgattgacta caacgatccc accgtgacaa ttgatttcta
    11821 ctacatcttc ccgcgtaaat gtctttggtg ctctcatcac ggagaccatg acttcatcga
    11881 caagctgatt gctcttggga tcaacgatat gtccataatg gatcgtatga cttggtactt
    11941 ctgataatcg tttgtttcca gattgataaa ctttatctgc tagttgcaca gcttgatcgc
    12001 cacttaaacg aacgatactg attgcacctt ctccaggtgg ggtagaaatt gccgcaatcg
    12061 tatcaaattc taaagtaatt tcagccattt tttcacttcc tttttcattt tgcgcacaaa
    12121 aaaagtgccc actccaccct ttatgcacaa taaacggtga acttcgcact ttgactgctt
    12181 gttcttgatt aaattctggt catagattat cacacttgct ataaggaatc aagtgtttaa
    12241 aacaattaga tgaagttgag cctgttctgc ttccattacc ttatatttaa ttgtccttgg
    12301 atgtctcttt gcgttctttg gtttattcga agagaagata ctgtataaac aaggctttat
    12361 ctttcttaaa aaaaacggta cactaaatgg tagcgctatc taaactatac acttttgaaa
    12421 ggagaaaata tgtgagcccg ttcagatctg ctgcattgga agaagaaagt gaccctcgga
    12481 tcaacgatgt cgttttatct acagggaata tcaaccagaa atatatcgtc cgcgatatcg
    12541 tttttgttgt ggaatgctta gaagctgatt tgtttgaccc atcttttccc aaagatgacc
    12601 ttttcttaac aacgaagagg aaattaaaga cgaaggtact tgaatatggt gcgaatgcag
    12661 taatcaactg tcattttgag tatcagcaaa aaaaacgcga cggaaagtat tttctggaac
    12721 tttttgctta tggaacagtg atccaattta tccaaacaac gattggttaa atgaaacaga
    12781 caaaaaaagc ggatcaaaac tgaagacagt ttcgatccgc ttttttctat caaagttagt
    12841 gactacttca tcgtatactc tgataagaat gatttctagt aaaatatact tggtttgaaa
    12901 aactttgtca acgtgattta ttcaaaaagt aatggaatca ataatatttt ttagcaggtt
    12961 ctactactaa ataacgataa ggttcctcac cttccgaatg tgtttttaca tgttcatctt
    13021 tgcttaatgc agcatggatt tgtttacgtt caaatgcagg cattggttct aagaaaacag
    13081 gctgccctgt ccgttttact ttttcagccg tgcgatcagc taaacgctga atgatctctt
    13141 ggcgcttttc acggtaattg cctacattga ctactacaga cagcttattt cttgctaaac
    13201 gatggatgaa gacttgtgcc agatattgca atgcattcag tgtttttcca tgtttcccaa
    13261 tcagtatgcc ttgcttttgt gtttctaaat ggaaaacaac catattattt gattctcttt
    13321 ccattttgac aagagctgga gcatcaagct gttttgaaat ttcagtaaga taaactgcca
    13381 attccgttaa agctgcttca tcacctagct cacttacttc ttctgttttt tcttcaagaa
    13441 tcgtttcgtt gactacctcg atctcagctg ttggttcttc ttccaaaatt tcttctacag
    13501 tctgttctac tgtttcagaa actttttcgc tgatacttgg ctggatcgaa agccgtgcat
    13561 tctttcgacc cattcctagg aaaccttttt ttccttcttc cagtatttcg atttcgactt
    13621 ggtctttggc caaacccaaa tcagccagac ctagttggac agcttcgtct attgttgcag
    13681 cttcataaat cggcattagc attacctccc ctattatttc cgttttttct taggattcat
    13741 tgctttctta agagcacgtt ctctttcgcg gattctccgt gcttcttctt cacgctcttt
    13801 tcgaattttg aaagggttgt tgatcagcag cgtttgtaca acttggaagg cattggaaac
    13861 tacccagtat aatgaaagac cgctggctaa attgacaccc atcactaaaa tcattacagg
    13921 catcacgaaa ttcatgatct tcattgatgc attcgattca atttgactca tgctagataa
    13981 atacgtactt gcaaaagtaa agattgctgc taaaatcggc aaaataaatg ttggatccgc
    14041 gttccctaag ttcaaccata agaaatgccc ttgtttcaac tctggtacac gtgaaataga
    14101 ttgccataac gccatcaaga ttggcatttg tactaataaa ggaagacaac cagcatatgg
    14161 attgacgcca ttttctgcat ataaacgctg ctgctcttct cttaacttac tttgtgtttc
    14221 cggatctctt gaactatatt gttgctgcag tgcttttaat ttcggttgta attcttgcgt
    14281 tttacgcatg ctctttgttt ggaaatgcat caacggcaat aggatcacgc ggatgatcag
    14341 tgtaaataaa atgatcccta tccctgcatt accaaatgac aacgccttga tcgcctcagc
    14401 aaagtaataa acaatataac gatcccaaat tcctgtactc tgtgcgctga cttccccagt
    14461 tccgcaaccg gataagacga tgaccaaact aacgataccg gccatcaaaa gtaaacgttt
    14521 gtatttcttc acttactctt ttcctctctg tcgattattt ttgcaagttt taagacgtgg
    14581 actaaattgg agcgaacctc atcataagtc aaccccttca cacttggtct agcaatcaca
    14641 ataaaatcca actccggatc aatttcttct tttatagaat aaactgctgc tcggattttt
    14701 cgtttgactt cattgcggtt taccgcgttg ccgacctttt ttcctacaga taaaccaact
    14761 cgaaaatgag gctgtcccgg tttttccaaa cggtaaacga cgaatttgcg gttagcaaaa
    14821 gaatctcccg tatggaacac ggcttgaaac tctcgttctt tttttactcg atacgctttt
    14881 ctcattagtt ttctttcctt ttctatctct cacttacgct cataatcata ccataattac
    14941 gattgtcctt tcatcatttc cctaaaaaag agtataaaaa aaaccactga aaccccagtg
    15001 gcctaagctg aaagaacttt tcttcctttg cgacgacggc tagctaacac acgacgtcca
    15061 tttttagtac tcatacgttt acggaatccg tgaacttttt gacgtttacg tttatttggt
    15121 tggtatgttc ttttcatata ttccacctcc aaaatgttct tatctatttt ataaacacag
    15181 acatactttg atagtataac gacaaagtag tgattttgtc aatataaaat agaagatcga
    15241 ttgttaaaac ttatccacaa gattattttt ttctatggga atcctatgtg gaaaaagtta
    15301 tgcacaggga ttatccacag ataataaaag gctggtttta cacagaaaac acacgtgtaa
    15361 aaaaagtaat ccacaatcag gcgatttcct gtgtattccg tctcctaact attggtatat
    15421 ctgtattttt tatccacaga cgcaagagga aatctattgg tttttacgag ttttccacct
    15481 gtgcattagt ggtgtggaaa actttttttg acaactgtgt ttttctcttt tttctttttg
    15541 tcgattgtgt ggataattgt aattaaaaaa tttttttctt cggtttctgt ggaaaactat
    15601 tgaaaaaaat gctaaattag tactagttat tcacttttta atgaaggagg aggccttata
    15661 tggtatccct cgatgcttta tggaatgaat taaaagcaac ataccaaaag gatttatcgc
    15721 ctgctagcta taatacatgg atcgaaacag cgaaccctcg aacccttgat caaaaccagc
    15781 tcgttgttga ggttcctagc aagattcata aagaatattg ggaaaaaaac ctggcgacaa
    15841 aaatcgttga agtcggttat atgttatcag gaaatgagat tatccctcgc tttattactg
    15901 gcgaagaagc agagcaagaa gaagttatag aagaaaaaaa tccaaaagtt gtggcaccta
    15961 gtccgctaaa aaaagccatg ctgaacccta aatatacctt tgatacgttt gtcattggta
    16021 aaggaaacca gatggctcat gcagcagcgc tcgttgtagc agaagatcca ggttctattt
    16081 ataacccgct cttcttttat ggaggcgtgg gacttggtaa gactcacttg atgcatgcaa
    16141 ttggtcatca aatgctgcaa agtcagccta atgccaaagt aaaatatgta agcagtgaaa
    16201 cttttgctaa tgatttcatc aattcgatcc aaaacaaaac agcagaagag ttccgtcaag
    16261 aatatcgaaa tgttgattta ctgctagtgg atgacattca attttttgca gaaaaagaag
    16321 ctacgcaaga agagtttttc catactttca atgccttgta caatgaaggg aaacaaatcg
    16381 tactaaccag tgatcggttg ccaaacgaga ttccgaaact gcaagaacgc cttgtttctc
    16441 gctttgcatg ggggctgtct gttgatatta caccgccgga tcttgaaaca agaacagcga
    16501 ttttacgtaa aaaagcggcg gctgaacgtc tagagatccc agatgacaca ctaagctaca
    16561 tcgcaggaca aattgattct aacattcgtg aattagaagg tgcgcttgtt cgagtgcaag
    16621 cttttgctgc gatgaatagt gaagatattt cgactagtct agctgcagac gcactgaaaa
    16681 cgttaaaatc aggaaaagga catcctcaat tgtcaatcct gcagatccaa gaagaagtgg
    16741 caaagtatta tcacatccaa ttgaaagatc tcaaaggaaa gaaacgcgtc aaatctattg
    16801 ttgttcctag acaaatcgct atgtatttgg cacgtgagct cacagataat tctttaccga
    16861 aaatcggagc tgaattcggt ggcaaggatc atacaacggt tatccatgct catgaaaaaa
    16921 tccagcagct gatggattct agtgtcagta tgcaaaatga agtatcagaa atcaaaaact
    16981 ttttactaaa ttaatttgtc gattctatca actgaaaata gcatgtggat aaaagaaaaa
    17041 aacgttcaaa agttatacac actttttcca cagctgtttt ttcgtttctc tcttacgttt
    17101 tttgagtttt ccacagaatc aacatgccct attactttta ttattctttt taactaataa
    17161 taaataaaca aacattcaaa ggagatacat catgaaagtt actttaaacc gagctagctt
    17221 tatgcaggaa ttgcaaactg ttcaacgagc tatttcaagc aaaaccacga tccctatttt
    17281 gacaggtgta aaaatcacac tgacacaaga aggtttgact ttgacgggga gcaacgctga
    17341 tatatcaatt gaaacttttt tgtctgttga aaacgaaaaa gcaaatatgc aaatcgaatc
    17401 tactggttcc attgttttac aagcacgttt ctttagcgaa atcattcgga gacttcctga
    17461 agaaacattt actttagaag ttttagaaaa taaacaagta gcgatcactt ctggaaaagc
    17521 gaattttatc gtaaatggat tagatgcaga taactatcct catcttcctg ttgtcgaaag
    17581 ccataaccag atgaaattac ctgtacacgt attgactaaa ctaatcaacg aaacagtttt
    17641 tgctgtctca caacatgaga gtcgtccaat cttgacaggt gtgcatttca ttttatctga
    17701 taattcttta ttagctgtag ctactgattc tcaccgtcta agtcaacgcg tgattccagt
    17761 agaacaagcg gctgatcatt ttgatattgt tattcctgga aaaagtttga tcgaattatc
    17821 tcgctcatta acaaatgaag aagaaatcgt cgaaatcagc attatggaaa accaagtgtt
    17881 gttcaaaaca gaaacgatgt atttctattc ccgtttgcta gaaggaaact atccagatac
    17941 caatcgtttg attccttcaa gttttaatac agaggttgaa ttttctgttc ctagcttttt
    18001 agcggcgatc gaacgggctt ctttactttc tcatgaaggc cgtaacaaca tcgttcgttt
    18061 atctattcgt ccagatgcgg ttgttttata cggaaattcg ccagaaatcg ggaaagtaga
    18121 agaaagcttg agttatacag caagcagtgg cgatccatta gatatttctt tcaatccgga
    18181 ttatatgaaa gcagcgttgc gtgcatttgg agatatgagc atcaaagtga aatttatctc
    18241 tgctattcgt ccatttacat tggaaccaac ggaagatggc gttcaattta ttcagctgat
    18301 tactcctgta cgtacaaatt aacaatgatt ttcaatgttc ttgaaaattt aattgaaaag
    18361 aaatagaaaa aataaaattt taaacagaag gccctaaaat ctcttttaag gctttctgtt
    18421 tatttttgtt taaatcacct tttttagatt aaatcgtctt atatggaaaa atatatggat
    18481 aaatttgttt tattgcgaaa aaaaaggtat aatagagtat acttttaata atgaaaatga
    18541 gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt tgggccaaat
    18601 gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt tggcagaaaa
    18661 cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat
    18721 gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa acgacgataa
    18781 tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat
    18841 tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg aatcttttgg
    18901 aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag caggaattga
    18961 ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt tcatccactc
    19021 ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac catatcgaac
    19081 agaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca ccagaagatc
    19141 tttctttagt aaaaggcagc ccgcagacca ggcggaagtt tttagatatg gaattaggcc
    19201 agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg aaacaaagaa
    19261 accagtactt gaagcaattg aatgaaaaaa aacaaactga tttgctttat ttggatattt
    19321 tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca cagttcgtca
    19381 agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa aaagagcagc
    19441 tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc
    19501 aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa
    19561 cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa aatgtgcaga
    19621 cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg
    19681 atttgattaa agaagagaca ggggaatatc caatcctttt gttagatgat gtgatgagcg
    19741 agctggatga taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt
    19801 tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa cctgagattt
    19861 tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag aaagaagttt
    19921 agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag gacttgaagc
    19981 cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc tgcatcacct
    20041 tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg caacgaaaat
    20101 ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc gggggatacc
    20161 tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta ctgttttaca
    20221 tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc acggagtagg
    20281 atcttctgtt gttaacgcgc tttctacaca gcttgatgtc aaagtataca aagaaggaaa
    20341 tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca
    20401 aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt ttacagagac
    20461 aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt tcttgaaccg
    20521 cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca atgaatacca
    20581 ttatgacggc gggattaaaa gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt
    20641 tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag tctctatgca
    20701 atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc atacgtatga
    20761 aggcggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa atgattatgc
    20821 ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag acgtgcgaga
    20881 aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag gacaaacaaa
    20941 aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta gcgaacattt
    21001 catgaaattc ttgctggaaa acccaagtgt gggccgtcaa attgttgaaa aaggattact
    21061 agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc
    21121 tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg atcctgaaaa
    21181 atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc aaggtcgtaa
    21241 tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag
    21301 catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta tgggtacagg
    21361 gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta tcatgactga
    21421 tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt atcgttacat
    21481 gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat acggggtaaa
    21541 acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag agttgaaaca
    21601 agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata aaggacttgg
    21661 agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc ggatgatggc
    21721 ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt tgatggggga
    21781 tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga agaacttaga
    21841 tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac atccatgatg
    21901 tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt gttattgtcg
    21961 cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt attttatacg
    22021 gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc agaatcgtcg
    22081 gagatgtcat gggtaaatac catcctcatg gagactatgc gatctatgag tcaatggtac
    22141 ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga aactttggtt
    22201 ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg agcaaaatcg
    22261 ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc aactatgatg
    22321 atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta gtgaacggaa
    22381 caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg tctgaggtag
    22441 tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa ttgatggaag
    22501 tattacctgg accagatttt ccaacaggtg gtttggttat gggaaaatca ggaatccgaa
    22561 gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag attactgaga
    22621 tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc aataaagcaa
    22681 aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg atcacagatt
    22741 tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca
    22801 gcgcatctgt tatcttgaat aacttatata aattgacatc cttgcaaaat tcatttggat
    22861 tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa caaattcttg
    22921 aaaactatat cgaacatcag cgcgaagtca tcacaagacg gacagtcttt gaaaaagaaa
    22981 aagcagaagc aagggcacac attttagaag gtttgcgtat tgctttggat catatcgatg
    23041 aaatcattgc gattatccgc gggtctaaat cagatgacga agcaaaagcg acgatgatcg
    23101 aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt ttacgccgat
    23161 tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta aaactgattg
    23221 aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc aagaatgaat
    23281 tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta gtcggtgagg
    23341 tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt acattgacaa
    23401 acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga aggggtggac
    23461 gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg gtttcttgtt
    23521 ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg gcaaaaggat
    23581 acgaaattcc agaatacggg cgcacagcca aaggaatacc aattatcaat ttactaggta
    23641 tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca gaagaaggcc
    23701 actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc actgcttttg
    23761 ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca
    23821 atgtgctact gacagatggt caatctaata tcattattgg aacacataac ggctactctg
    23881 tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg gttagaggaa
    23941 tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa gatcaagaag
    24001 tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa tatccagtga
    24061 aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag
    24121 ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat aaaggtgtca
    24181 ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta ggcgtacgtt
    24241 tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa ccagaagctg
    24301 cagaacaaac agttgaagaa caaggatcag aagaatagcc atcaagacta attgtgtaag
    24361 ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc tggctttttt gattagttga
    24421 atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa aatagagtgt
    24481 ataatcagag cttgtgagta gtctaattaa ttagataact ctccttgctc ttaggaacct
    24541 aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata tgaaatcatg
    24601 tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat
    24661 gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga aaaacgccgt
    24721 ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa agtatcttct
    24781 ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc
    24841 attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa acaatagaaa
    24901 acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac gtctgacgaa
    24961 agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta ctttggctgt
    25021 gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca actgtgtgat
    25081 ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt
    25141 tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg tatacgtgac
    25201 agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca
    25261 acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg gaaacaataa
    25321 ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg atacttctga
    25381 cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat tctaaacgaa
    25441 tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag
    25501 tagactacat tgctgctaac catattgaat atatcgatta taaagatatc gaattactaa
    25561 aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta
    25621 aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc ttgctacctt
    25681 tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg atcagctatt
    25741 caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa tgacgaaagt
    25801 tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa aaacaatcat
    25861 ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt ggtagtctta
    25921 attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt atggggtgtt
    25981 cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa aatccagcag
    26041 gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc tgtaggagtg
    26101 attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc attgaaaatc
    26161 aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac tgcaaaagaa
    26221 aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat ggatacagta
    26281 aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact ccatcagacg
    26341 aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc cattgataaa
    26401 ttggatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt ttctgactgg
    26461 atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt cttcgtagca
    26521 cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga tcgctttcga
    26581 gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc ttatggctca
    26641 agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc tttagtccgt
    26701 ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt ttattatggg
    26761 gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct
    26821 ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa gtttccggat
    26881 atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa
    26941 gcttacatcg ttatcaacga gaacgagatc attccagatg tagaacggtg tcttgaagaa
    27001 atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc catagagctg
    27061 aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct atcgatctct
    27121 cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg tcgtggggaa
    27181 gagtttccaa gtaaacctct gctgacttat atcgaatctt cagcttcttc agcttcagag
    27241 ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga tcgttttgag
    27301 gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt ccgtacaact
    27361 gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc atccttagta
    27421 caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt gattgctgga
    27481 agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa agagtataat
    27541 agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat caatgcagct
    27601 ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag taccggcacg
    27661 atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac aaacgcagct
    27721 actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc tgcaatcaaa
    27781 gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt tttttacaag
    27841 atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa
    27901 attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc agtcagttag
    27961 caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt
    28021 taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag
    28081 acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta aacaaacaat
    28141 acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt tcactaggct
    28201 acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg
    28261 tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca gaaggaaaaa
    28321 agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc
    28381 gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa agaaaacgag
    28441 tataataaaa gagaactatt tttttattac actttcggta cgaatgatga cttttctctc
    28501 attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat gaagtgtggc
    28561 aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc tctgtttttc
    28621 ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac ttttatcgtc
    28681 gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat gaagcgattg
    28741 acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat gccggtggtt
    28801 taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc gtctactatg
    28861 cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca acaggaatcg
    28921 ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat gccgaacgca
    28981 gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt gctgaagtac
    29041 tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa atcacaggtc
    29101 taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc gaagaactaa
    29161 tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat attgctcaaa
    29221 atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg ggggcagaat
    29281 ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat ttacgtacag
    29341 gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt ctttctcgag
    29401 caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc
    29461 ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat ttgcagttga
    29521 ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct cgccaactga
    29581 aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta tctcgtggag
    29641 tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg
    29701 aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa agagaaggcg
    29761 gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc atcatcgaga
    29821 agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa tacaataagt
    29881 tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc
    29941 cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag tacttaattt
    30001 aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa
    30061 cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata tgaggaacta
    30121 ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac ctcttttcct
    30181 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca aacatgagaa
    30241 gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttt
    30301 cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg
    30361 ag
//
LOCUS       DACEJZ010000028        29111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEJZ010000028 DACEJZ010000000
VERSION     DACEJZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.028"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1031)
                     /locus_tag="IW656_001140"
     CDS             complement(<1..1031)
                     /locus_tag="IW656_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0836859.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IW656_001141"
     CDS             complement(1113..1802)
                     /locus_tag="IW656_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0836860.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW656_001142"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IW656_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0836861.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IW656_001143"
     CDS             complement(2329..2832)
                     /locus_tag="IW656_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0836862.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW656_001144"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IW656_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0836863.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW656_001145"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IW656_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0836864.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW656_001146"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IW656_001146"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0836865.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW656_001147"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IW656_001147"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0836866.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IW656_001148"
     CDS             complement(6444..7463)
                     /locus_tag="IW656_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0836867.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IW656_001149"
     CDS             complement(7636..8523)
                     /locus_tag="IW656_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0836868.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW656_001150"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IW656_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0836869.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW656_001151"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IW656_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0836870.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW656_001152"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IW656_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0836871.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IW656_001153"
     CDS             9754..10398
                     /locus_tag="IW656_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0836872.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IW656_001154"
     CDS             complement(10454..10957)
                     /locus_tag="IW656_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0836873.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IW656_001155"
     CDS             11554..12519
                     /locus_tag="IW656_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0836874.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IW656_001156"
     CDS             complement(12618..13790)
                     /locus_tag="IW656_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836875.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IW656_001157"
     CDS             complement(13777..14922)
                     /locus_tag="IW656_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0836876.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IW656_001158"
     CDS             complement(14936..15868)
                     /locus_tag="IW656_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836877.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IW656_001159"
     CDS             15985..17064
                     /locus_tag="IW656_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0836878.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IW656_001160"
     CDS             17061..17690
                     /locus_tag="IW656_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836879.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IW656_001161"
     CDS             complement(17800..18027)
                     /locus_tag="IW656_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0836880.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IW656_001162"
     CDS             complement(18130..20718)
                     /locus_tag="IW656_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0836881.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IW656_001163"
     CDS             complement(20738..21397)
                     /locus_tag="IW656_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0836882.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW656_001164"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IW656_001164"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0836883.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IW656_001165"
     CDS             complement(22170..23036)
                     /locus_tag="IW656_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0836884.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IW656_001166"
     CDS             23145..23786
                     /locus_tag="IW656_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0836885.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW656_001167"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IW656_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0836886.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IW656_001168"
     CDS             complement(25227..26126)
                     /locus_tag="IW656_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0836887.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IW656_001169"
     CDS             complement(26128..26925)
                     /locus_tag="IW656_001169"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0836888.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IW656_001170"
     CDS             complement(26903..27607)
                     /locus_tag="IW656_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0836889.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IW656_001171"
     CDS             27738..28313
                     /locus_tag="IW656_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0836890.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IW656_001172"
     CDS             28431..29108
                     /locus_tag="IW656_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0836891.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6169 c         5232 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agcttatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa a
//
LOCUS       DACEJZ010000029        28598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEJZ010000029 DACEJZ010000000
VERSION     DACEJZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.029"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            236..1876
                     /locus_tag="IW656_001173"
     CDS             236..1876
                     /locus_tag="IW656_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0836892.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="IW656_001174"
     CDS             2030..3352
                     /locus_tag="IW656_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0836893.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="IW656_001175"
     CDS             complement(3414..3845)
                     /locus_tag="IW656_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0836894.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="IW656_001176"
     CDS             complement(3909..4745)
                     /locus_tag="IW656_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ0836895.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="IW656_001177"
     CDS             complement(4935..5237)
                     /locus_tag="IW656_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0836896.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="IW656_001178"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="IW656_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0836897.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="IW656_001179"
     CDS             complement(5691..5957)
                     /locus_tag="IW656_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0836898.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="IW656_001180"
     CDS             complement(6211..6936)
                     /locus_tag="IW656_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0836899.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="IW656_001181"
     tRNA            complement(7302..7372)
                     /locus_tag="IW656_001181"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="IW656_001182"
     CDS             7489..8241
                     /locus_tag="IW656_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0836900.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="IW656_001183"
     CDS             8257..8736
                     /locus_tag="IW656_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0836901.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="IW656_001184"
     CDS             complement(8987..10567)
                     /locus_tag="IW656_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0836902.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="IW656_001185"
     CDS             complement(10560..11303)
                     /locus_tag="IW656_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836903.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..12255)
                     /locus_tag="IW656_001186"
     CDS             complement(11479..12255)
                     /locus_tag="IW656_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836904.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12503..13339)
                     /locus_tag="IW656_001187"
     CDS             complement(12503..13339)
                     /locus_tag="IW656_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0836905.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13350..14279)
                     /locus_tag="IW656_001188"
     CDS             complement(13350..14279)
                     /locus_tag="IW656_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0836906.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14471..14824
                     /locus_tag="IW656_001189"
     CDS             14471..14824
                     /locus_tag="IW656_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0836907.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14898..15554)
                     /locus_tag="IW656_001190"
     CDS             complement(14898..15554)
                     /locus_tag="IW656_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0836908.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15780..17714
                     /locus_tag="IW656_001191"
     CDS             15780..17714
                     /locus_tag="IW656_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0836909.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17800..19023)
                     /locus_tag="IW656_001192"
     CDS             complement(17800..19023)
                     /locus_tag="IW656_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0836910.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19017..20915)
                     /locus_tag="IW656_001193"
     CDS             complement(19017..20915)
                     /locus_tag="IW656_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0836911.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20909..22027)
                     /locus_tag="IW656_001194"
     CDS             complement(20909..22027)
                     /locus_tag="IW656_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0836912.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22110..22943)
                     /locus_tag="IW656_001195"
     CDS             complement(22110..22943)
                     /locus_tag="IW656_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0836913.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22936..23853)
                     /locus_tag="IW656_001196"
     CDS             complement(22936..23853)
                     /locus_tag="IW656_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836914.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24080..25204)
                     /locus_tag="IW656_001197"
     CDS             complement(24080..25204)
                     /locus_tag="IW656_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0836915.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25387..26295
                     /locus_tag="IW656_001198"
     CDS             25387..26295
                     /locus_tag="IW656_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0836916.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26436..28049
                     /locus_tag="IW656_001199"
     CDS             26436..28049
                     /locus_tag="IW656_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0836917.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8889 a         5621 c         4868 g         9220 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    11641 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    11701 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    11761 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    11821 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    11881 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    11941 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12001 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12061 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12121 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12181 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12241 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12301 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12361 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    12421 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    12481 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    12541 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    12601 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    12661 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    12721 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    12781 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    12841 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    12901 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    12961 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13021 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13081 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13141 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13201 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13261 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13321 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13381 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    13441 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    13501 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    13561 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    13621 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    13681 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    13741 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    13801 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    13861 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    13921 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    13981 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14041 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14101 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14161 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14221 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14281 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14341 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    14401 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    14461 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    14521 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    14581 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    14641 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    14701 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    14761 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    14821 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    14881 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    14941 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15001 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15061 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15121 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15181 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15241 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15301 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15361 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    15421 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    15481 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    15541 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    15601 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    15661 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    15721 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    15781 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    15841 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    15901 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    15961 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16021 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16081 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16141 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16201 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16261 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16321 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16381 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    16441 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    16501 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    16561 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    16621 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    16681 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    16741 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    16801 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    16861 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    16921 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    16981 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17041 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17101 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    17161 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17221 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17281 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17341 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    17401 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    17461 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    17521 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    17581 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    17641 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    17701 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    17761 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    17821 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    17881 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    17941 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18001 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18061 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18121 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18181 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18241 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18301 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18361 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    18421 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    18481 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    18541 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    18601 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    18661 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    18721 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    18781 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    18841 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    18901 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    18961 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19021 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19081 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19141 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19201 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19261 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19321 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19381 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    19441 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    19501 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    19561 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    19621 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    19681 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    19741 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    19801 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    19861 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    19921 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    19981 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20041 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20101 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20161 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20221 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20281 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20341 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    20401 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    20461 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    20521 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    20581 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    20641 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    20701 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    20761 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    20821 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    20881 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    20941 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    21001 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    21061 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    21121 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    21181 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    21241 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    21301 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    21361 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    21421 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    21481 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    21541 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    21601 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    21661 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    21721 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    21781 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    21841 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    21901 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    21961 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    22021 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    22081 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    22141 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    22201 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    22261 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    22321 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    22381 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    22441 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    22501 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    22561 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    22621 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    22681 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    22741 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    22801 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    22861 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    22921 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    22981 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    23041 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    23101 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    23161 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    23221 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    23281 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    23341 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    23401 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    23461 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    23521 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    23581 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    23641 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    23701 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    23761 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    23821 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    23881 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    23941 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    24001 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    24061 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    24121 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    24181 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    24241 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    24301 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    24361 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    24421 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    24481 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    24541 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    24601 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    24661 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    24721 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    24781 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    24841 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    24901 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    24961 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    25021 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    25081 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    25141 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    25201 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    25261 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    25321 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    25381 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    25441 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    25501 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    25561 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    25621 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    25681 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    25741 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    25801 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    25861 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    25921 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    25981 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    26041 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    26101 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    26161 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    26221 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    26281 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    26341 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    26401 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    26461 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    26521 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    26581 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    26641 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    26701 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    26761 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    26821 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    26881 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    26941 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    27001 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    27061 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    27121 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    27181 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    27241 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    27301 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    27361 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    27421 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    27481 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    27541 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    27601 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    27661 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    27721 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    27781 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    27841 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    27901 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    27961 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    28021 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    28081 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    28141 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    28201 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    28261 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    28321 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    28381 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    28441 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    28501 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    28561 tgtattaaat aatttaattt ttttgttcct atttattt
//
LOCUS       DACEJZ010000030        27998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEJZ010000030 DACEJZ010000000
VERSION     DACEJZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.030"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW656_001200"
     CDS             complement(5..586)
                     /locus_tag="IW656_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0836918.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW656_001201"
     CDS             complement(769..1395)
                     /locus_tag="IW656_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0836919.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW656_001202"
     CDS             complement(1417..2748)
                     /locus_tag="IW656_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0836920.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW656_001203"
     CDS             complement(2903..3304)
                     /locus_tag="IW656_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0836921.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW656_001204"
     CDS             complement(3390..3596)
                     /locus_tag="IW656_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836922.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW656_001205"
     CDS             complement(3691..5193)
                     /locus_tag="IW656_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0836923.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW656_001206"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW656_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0836924.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW656_001207"
     CDS             complement(5764..6381)
                     /locus_tag="IW656_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836925.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW656_001208"
     CDS             6602..7975
                     /locus_tag="IW656_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0836926.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW656_001209"
     CDS             8050..9162
                     /locus_tag="IW656_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0836927.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW656_001210"
     CDS             complement(9234..10871)
                     /locus_tag="IW656_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0836928.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW656_001211"
     CDS             complement(11039..11647)
                     /locus_tag="IW656_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0836929.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW656_001212"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW656_001212"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0836930.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IW656_001213"
     CDS             complement(13201..13953)
                     /locus_tag="IW656_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836931.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IW656_001214"
     CDS             complement(14094..15044)
                     /locus_tag="IW656_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836932.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IW656_001215"
     CDS             complement(15045..15884)
                     /locus_tag="IW656_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0836933.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IW656_001216"
     CDS             complement(15881..16627)
                     /locus_tag="IW656_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836934.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IW656_001217"
     CDS             16865..18235
                     /locus_tag="IW656_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0836935.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IW656_001218"
     CDS             complement(18290..19186)
                     /locus_tag="IW656_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0836936.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IW656_001219"
     CDS             complement(19467..20144)
                     /locus_tag="IW656_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0836937.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IW656_001220"
     CDS             complement(20235..21140)
                     /locus_tag="IW656_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0836938.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IW656_001221"
     CDS             21315..22769
                     /locus_tag="IW656_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0836939.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGVIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IW656_001222"
     CDS             22785..23816
                     /locus_tag="IW656_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0836940.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IW656_001223"
     CDS             23823..25010
                     /locus_tag="IW656_001223"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0836941.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IW656_001224"
     CDS             25166..26548
                     /locus_tag="IW656_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0836942.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IW656_001225"
     CDS             26675..26905
                     /locus_tag="IW656_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836943.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IW656_001226"
     CDS             complement(27105..27773)
                     /locus_tag="IW656_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0836944.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7791 a         5726 c         5148 g         9333 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcgtcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggacg
//
LOCUS       DACEJZ010000031        27651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEJZ010000031 DACEJZ010000000
VERSION     DACEJZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.031"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(135..680)
                     /locus_tag="IW656_001227"
     CDS             complement(135..680)
                     /locus_tag="IW656_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0836945.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(692..1384)
                     /locus_tag="IW656_001228"
     CDS             complement(692..1384)
                     /locus_tag="IW656_001228"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0836946.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1403..1687)
                     /locus_tag="IW656_001229"
     CDS             complement(1403..1687)
                     /locus_tag="IW656_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836947.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1700..2263)
                     /locus_tag="IW656_001230"
     CDS             complement(1700..2263)
                     /locus_tag="IW656_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0836948.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2491..3153
                     /locus_tag="IW656_001231"
     CDS             2491..3153
                     /locus_tag="IW656_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0836949.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3277..4473
                     /locus_tag="IW656_001232"
     CDS             3277..4473
                     /locus_tag="IW656_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0836950.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4574..5950)
                     /locus_tag="IW656_001233"
     CDS             complement(4574..5950)
                     /locus_tag="IW656_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0836951.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5987..6853)
                     /locus_tag="IW656_001234"
     CDS             complement(5987..6853)
                     /locus_tag="IW656_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0836952.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6942..7967)
                     /locus_tag="IW656_001235"
     CDS             complement(6942..7967)
                     /locus_tag="IW656_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0836953.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7985..8659)
                     /locus_tag="IW656_001236"
     CDS             complement(7985..8659)
                     /locus_tag="IW656_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0836954.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8843..9736)
                     /locus_tag="IW656_001237"
     CDS             complement(8843..9736)
                     /locus_tag="IW656_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0836955.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9810..11507)
                     /locus_tag="IW656_001238"
     CDS             complement(9810..11507)
                     /locus_tag="IW656_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0836956.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11500..12813)
                     /locus_tag="IW656_001239"
     CDS             complement(11500..12813)
                     /locus_tag="IW656_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0836957.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12813..16850)
                     /locus_tag="IW656_001240"
     CDS             complement(12813..16850)
                     /locus_tag="IW656_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0836958.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16863..17321)
                     /locus_tag="IW656_001241"
     CDS             complement(16863..17321)
                     /locus_tag="IW656_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0836959.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17619..18137)
                     /locus_tag="IW656_001242"
     CDS             complement(17619..18137)
                     /locus_tag="IW656_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0836960.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18139..18756)
                     /locus_tag="IW656_001243"
     CDS             complement(18139..18756)
                     /locus_tag="IW656_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0836961.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18753..19616)
                     /locus_tag="IW656_001244"
     CDS             complement(18753..19616)
                     /locus_tag="IW656_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0836962.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19603..20514)
                     /locus_tag="IW656_001245"
     CDS             complement(19603..20514)
                     /locus_tag="IW656_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0836963.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20555..21889)
                     /locus_tag="IW656_001246"
     CDS             complement(20555..21889)
                     /locus_tag="IW656_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836964.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22097..22927
                     /locus_tag="IW656_001247"
     CDS             22097..22927
                     /locus_tag="IW656_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ0836965.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23171..23420)
                     /locus_tag="IW656_001248"
                     /pseudo
     CDS             complement(23171..23420)
                     /locus_tag="IW656_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23611..23805)
                     /locus_tag="IW656_001249"
     CDS             complement(23611..23805)
                     /locus_tag="IW656_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836966.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24310..24495)
                     /locus_tag="IW656_001250"
     CDS             complement(24310..24495)
                     /locus_tag="IW656_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836967.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25345..25686)
                     /locus_tag="IW656_001251"
     CDS             complement(25345..25686)
                     /locus_tag="IW656_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0836968.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25686..25916)
                     /locus_tag="IW656_001252"
     CDS             complement(25686..25916)
                     /locus_tag="IW656_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0836969.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26203..26541
                     /locus_tag="IW656_001253"
     CDS             26203..26541
                     /locus_tag="IW656_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836970.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26746..26985)
                     /locus_tag="IW656_001254"
     CDS             complement(26746..26985)
                     /locus_tag="IW656_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ0836971.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26961..27308)
                     /locus_tag="IW656_001255"
     CDS             complement(26961..27308)
                     /locus_tag="IW656_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0836972.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         8224 a         5888 c         4721 g         8818 t
ORIGIN      
        1 aagagattta ttaacggaaa aagaataagt ttttttaaaa taacgtataa aaaagagcat
       61 aaaacaaagt cgtttgtttt atgctcttct tttattattt tgtgcttttt attttatcaa
      121 atcactttgt ttttttataa aatttctgcg cctagtgtgg actcaaaatg tctcaatgcc
      181 cattcatgtc caacgggatc gaaacttgcc acggaatctt tgaaaatatg gagtttgtat
      241 cctagattat acgcatcgac tgctgtatgc aacacacaga tatctgtaca tacgccggtc
      301 aaataaatat ctgtgatctg gcgttctcgc aaacggatgt ctagatctgt tccactaaaa
      361 gcggaataat gccgtttgtc tatccaataa acattttcct cttgctcata gtcttggtat
      421 aaacttttta aagagccgta caattcccgt ccacccgttc ctatcacatt atgcggagga
      481 aataagcggt tctccggatg aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg
      541 acaaaatcct tttgatcaat aaacttcttt gtatgagcag tcagcttttt ttcaattact
      601 tgtcctgctg tcccagtagt cagctttcca tcctctgcaa caaaatcata tgtgtagtca
      661 atcgagatca atgcgttcat cttagtcctc cttatacgag atgtttgaca aattcgatca
      721 ccatttctgc tgatcgtttg cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg
      781 agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa tgggatattg aactgacgag
      841 ctgtttgtcc aacagcggct ccttccattt cgcaagctaa tgcttcaggg aaattcgtta
      901 agatttcttt gattttatct ggcgagtcga caaatgtatc acctgtaacg atcaatcctt
      961 cttttgcgtt caaattcgtt gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc
     1021 ttgcttcata atatagaggc attcctggta attgtcctgg gttgtagcca aatcctgttg
     1081 catctgcatc aaaataagct actttgtctg atataacgat gtcacctact tgtaagcctt
     1141 caccgattcc gccagctgaa cctgtattga tcaccatgtt cacgccgtat tgttggatca
     1201 acagacttgt ggtaatagat gctaagactt ttcctatacc ggaacgtacg acgatcactt
     1261 cgtggttacc taatgaacct gaaataaata aagcgcctgc gcgttcccat gataatggtt
     1321 cgcttaggtt ctctctcaaa atttttactt cttcttccat tgcgccgata ataccgattt
     1381 tcatcttttt aattccttct ttctatataa atgcgattgc taaaaaaacc acacataaca
     1441 ttaggataac aataataaga gatttcatca aaaaagagtt ccatttggtc gtcttttccc
     1501 gttcgctgat ccttgttttc gtgattggtt tattcttttt gctttcttta cgataaaagc
     1561 tggcaatctt tttttcttct tgctgataag ctgtttctgc ttttcgctgt ttttttaatg
     1621 actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt
     1681 tggccataag ccgatccctc tactttcctt tggtcaatag ttcccagtat tgaatcgcat
     1741 aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag
     1801 ttagatgata caactccagt acttcgtctt catcttgtgc taaaggattc tcgaccttct
     1861 ctaccccttg tgcatgataa atatgaagga cttcatttgc aaaacctggt gaaagataca
     1921 tcgaagtaag atggctcagc gacttcgcac gatagccagt ttcttcttct aattctctag
     1981 ctgctgtcat ttcgggattt tgtccttctc ctggatcgat ttttccagct ggaatctcca
     2041 aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag aagcagacga tcttgttcgt
     2101 caaaagcaat gatcccgaca ccgccaggat gaaataccag ttctctcttc gaagttcctc
     2161 catctggcaa acgaacttcg tctacagcaa catcaataat ttttccgcta tagatttctt
     2221 tgcgactgat tgttttttct tcaaattgct cattgttcag catatcttct cctgctttct
     2281 ttgtaatcat aactgctttt ttttcatttt tcaatttttc tcaaaaactc cttattagtt
     2341 taccatagat aaaccttaga ttttctctaa atatagaaaa tccttcttta gtccgatact
     2401 ctttctagta gtccgtatgc taaaataagt atgtaatcga ataacgaaaa gaattcaata
     2461 ctcaacgata aatgacatgg agggtttatt atgataaaac gaaattggcc gtggcttcta
     2521 gcattggtct tgctatttct ctttgtccga aacaatagtt tttcttttgt tctaggactt
     2581 ttattgatag gtgtagtttt agttttgtgg ggactctttg gttcgcgcag gaaaaaggga
     2641 aaacaagaag aaattcctgc actgactgac gaacttgaaa ctcactatga aaaaagcggt
     2701 atgacagcga atgaaatttc attttttcga caaacaatga atcaaacaaa aaatgagatc
     2761 aatcaattgc aaatgaatat gcagcagaca gccaaactta aagcaatcga tctccgccat
     2821 gatccagtca aagcagcgaa agctttattc aaagaattgg tgaaagaacc aaaccgactg
     2881 catgaagcaa gtcagtttct ttatacgcat ctgccgaatc tagttgattt gacaaataag
     2941 tataatgaga tcaatgacca tgagatcaaa aacaaacaga cctatgaaaa actagaagaa
     3001 agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac aagattatca aaaattcgtt
     3061 gctgaggatc tagatgatat cgacgtagag atatctgttg cgaaacagag cttaaagcgt
     3121 gataataaat atgaaccaga acaaaaggat taactctttt gttcataaaa gaagactcgg
     3181 aacattcaga tgatgtctgg ctgtttcgag tactttttct aaagtagaga cattcatgta
     3241 agaatcgaga acagattcaa caaggaggaa ttttttatgg atcataaacc gactgaaaca
     3301 aaagatgtta caccagttaa cgatacactt gaagatttat tgaacaaccc gttttcaact
     3361 ccaacagata cgctgaccac ttcacaacaa gcggagattg atcagctcca agagcaacag
     3421 acagctgctc gtttaattga caaactaccg gcggaacgcc aagaacaagc taaacagcta
     3481 gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca gctacggatc ggctgcccaa
     3541 gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg tgcaagcaca agatattggt
     3601 cctgtaggag attccttaac tgaactgatg tatcgtctcc aagaagccaa tcctgacgaa
     3661 ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg ggaaggtcaa acaatcgatc
     3721 tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa ttgacaagat ttcagtgaaa
     3781 ttagataaag aaaaagacgg tctgctaaaa gataatctga tgttagaaca gctttaccag
     3841 aaaaacaaag actactttga tgcactgaat atttatatcg ctgctggaga attgaaaatg
     3901 gaagaacttc aaacaacgat cattccagaa gcgatgaaaa gagcagaaga aactggcgat
     3961 cagatggatg ttcagattgc caatgattac acgcaattcc tagatcgtct ggataaacgt
     4021 acgcatgatc ttcgcttagc tagacagatc acgatccagc aagcaccaca aatccgtttg
     4081 atccaaaaca caaatcaggc cttggctgaa aagatccaag catctatcgc tacagccatt
     4141 ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc ttcgtcaaaa agatgcagta
     4201 actgctcaac gccaagtctc tgaaacaaca aacgacttat tgaagaaaaa ttcagaaatg
     4261 ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac gtggaatcgt ggatatcgag
     4321 acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc aagagacatt gcgtatccaa
     4381 aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc ttggacacat ggaagaagat
     4441 ctgaaacaaa aactgttgga tttaacccaa tagatcacct ttcgcttgtt actaaaacaa
     4501 acgcccaaca acagtttatt acaactgttg atgggcgttt ttcttatctt tcttctaact
     4561 tctccattgg agtctatttc tcattcatct tgctgtgtcc atagccatag ccaaaataaa
     4621 tgatcagtcc aataaccatt acgataccga acacgatcca tgtcacagtt tgcagacgaa
     4681 gcatcaatac aatacaagtc aagatggaga cgattggcaa tagcggaacg aatggcactt
     4741 tgaattctcc agcttttggt ttgccaaaca tttttctcaa cttcagtaag cctagactaa
     4801 caagcatcaa ataggcgagc gccatgatat tcgttagttc cgctaataca ttcaatggga
     4861 aaaatccggc aaagaataca gctgccacac cagcggcata agtcgctttt ttcggtgtac
     4921 gatgcttttg atcgatttct gtcataaact taggaagtaa gccatccttg ctgacagcaa
     4981 acagtaaacg agcaagtgaa tacatcatcg agatcgttac cgtcagtagc gttaaaattg
     5041 cccctacagc gatcaccgcg cccactgttc catgtcctac atacctcatt gcaaaagcca
     5101 ctgggtcatt gactcctagt tcagtatacg gaacgattcc tgtcaagatc agcgtcacga
     5161 tgatgtaaag gacagtagca atcagtatcg aaccaatgat tcctcttggc acatcttttt
     5221 gcgggttttt gacttcctcg gctgccattg caacggcatc aaatcccaag aaagcaaaaa
     5281 acacaagagc tgcaccatct agcacaccgg aaaaaccaaa aggcatgaat ggctgccagt
     5341 tatcaggttt aacgaaaaat attcctacaa tgataaacaa cgcgatgata ccaaatttga
     5401 catataccat ggcattattc aaacgtaatg ctttcttggc ttcaagcgac acgatgtaag
     5461 tgacgaatat cactacgagc accgcaatca agtcaatgta tgtaccattt tccggattgt
     5521 acccgccagt taatgcttgt ggaagttcgg cccctaaact tttcaaaaaa ccttgcatat
     5581 agccagacca tccggaagca acagaggaaa ccgctagtaa aaattccccg atcaacaacc
     5641 accctgtcag ccaggcagca aattcaccaa atacaacata cagataggca tatggcccac
     5701 caatcactgg cactcgcgaa gcaaattctg cgaaacttag tccagacaaa atgacaacga
     5761 ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc attttgattg gccgcaaccc
     5821 ctgtcaccac aaatattccc gtaccgatga tcgcaccaat tccaagcatg atcaaatcag
     5881 acgtttttag ttcttttttc atctcactcg tatgattgag attgatctct ttcttacgaa
     5941 acaaatccat tgaagttcct ccccatttta ggttggaaaa cagcgattaa actgttttca
     6001 tataccctaa aaggatacca cctaaaatga ctaaaaggca accaacaata acataaatga
     6061 gctctttttt cgtttttctt tcgccaagga tgaaaatccc acctaaagta gagataatga
     6121 tccccatttg tgaaagtgag aagccaacag ctaaaccaag tgatttgact gttagaagca
     6181 tgcagatgtt tcctaagccc cataataatc cacagatcat gtttttccac acaaactgat
     6241 cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa gatcattccg atactttgag
     6301 gtaagataat tgacatcgca tccaaattcg cccagttgac gatgatggta tatacaccat
     6361 aaccaatcgt agagaagatc aatgctctaa aacccttacc aaaatcaagc attttattat
     6421 ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa atacgcaccg cttacaagaa
     6481 gagctaaagc aataataccg tatacataat ctttcgtttg tgtccattca tggaagaaaa
     6541 cggctcccgc aatcgtattc aaaattaatt gaaaaccagt agacaatggt agccctacag
     6601 aaacacccat gtacttcatt ccatggaact gtccattttg tccaacactc caagctaagc
     6661 cggataaaaa tccgatcaag aaaatccaag gggtgattac aggactaaca acgaaaaaga
     6721 caactaaaga aaacagaaat gcaccgatcg tcattcctag tgtttgttga ttggcatcgc
     6781 caccaatttt accacttact aaaccaatac taccccatgc caacataggg actaatgcta
     6841 ttaaaatttc cataaccaat ctccttttat ttcacatgat aagaaactta tcagcgaaaa
     6901 cgttaacaac acaagcactt tttttcgttt ctaacgattt tctaacgttt ttcattataa
     6961 aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt ttccttcagt tgattcagcc
     7021 gtcaaatgga tacctaattt ttcggccaga ttttttgcta aataaagtcc taatcctgtt
     7081 gaatgtgttt tgctaagtcg accatttctc ccagtaaatc ccttatcaaa gatccgtttc
     7141 aagtcttccg ctgggatacc gatccctgaa tctttaactg aaaggctgac tccttgcgct
     7201 tccttttcta tgctcacaat gatgtttccg tgatccggcg tatatttgat ggcattacta
     7261 agcaattggt tgaagataaa agcgatccat ttagcatcgg tcaaaacagt atgatcctca
     7321 ccaacgatct cgtagcgaat atttttttgg ataaaataat ttgcctgact gcgtatcaca
     7381 ggctgaatga ttccttttag tccatattct tggatgaggt agtcccgcga gaaactgtcg
     7441 agtcttgcat aatacaaaac ttgttctacg tattcatcga tttttgataa ttcattttct
     7501 agtaatgtat acttttgatc gtcaatatcg aactcgatcg aacgaactag caatttagaa
     7561 gcggaaagtg gcactttgat ttcatggacc catgagtcaa tataatcttt ttggtcttgt
     7621 tgattactga ttgctgattg cattacttct tggtgttcac ggatcagctg attgatatat
     7681 tcttctacca gtttttcttc ttctgtcctc ccaccactca aataattttg caaaggggaa
     7741 gttttttgta tgtcggctaa ttttccccac catcttctct tgctcaagta accacccagc
     7801 agataaaaaa tcagaaagac gccctcaatc aaagctaaat aagcaattgt gctcaaatca
     7861 actttgatgt ttggcgccag ccacatcaca aagatcgtca atgcaataaa aaacagccat
     7921 cccaagatca atgtccactg atctttcaaa tatttaaaaa aattcatctt aaccgctcat
     7981 cctttcaggg aacgatatat ccctgtccta cttttgtttc gatatatcct tggatacctg
     8041 cctgttcgat ttttcggcgt aaccgattga tgttgactgt caatgtattg tcatctacaa
     8101 atcgttcgtc atcccacagt gctcgcagca atttttcccg acttaagatc tttccattgt
     8161 gcttcatcaa aataaataat agacgatatt catttttgct caaatcaatg atctctccgt
     8221 tgatttccat acttccgttg tcaacattca acgtaatgcc attgtggctc atcatttcac
     8281 tagataattc tgtgtagttg taagaacggc gaagtaatgc attgattttt gcaatcagta
     8341 catcgatttg gaaatgtttt gtcacaaaat catctgcccc catgttcata gccatgatca
     8401 tatccatatt cgtattcctg ctggagataa aaatgatcgg tactttcgat acttcacgta
     8461 ttttttgaca ccaataatag ccatccaaaa cgggaagatt aatatctagt aatacaagct
     8521 gtggttcttc tctttcaaag tctgagaaca cttgattgaa atcggtcgtt ccaaaggttt
     8581 caaattgcca tttctcaagt tcctccatga tcaattgtcg aatgatctct tcgtcttcga
     8641 ctaccatgat ttttgccata aaaaatccct ccatctttct tcttgctact acaatatcaa
     8701 agaagccctt gtgaaacaag ttggcttacc tggaaaatgt cattttagca aacgttaaaa
     8761 aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc aagcagctta aatttattat
     8821 gttattctta ataattggtc tattatctgt tatttttctt tcgttgtttt tgtcttttaa
     8881 attttcgata acaccaataa ccgaaaagcg ttagcactag caatatacct agaaggagta
     8941 taagtaatcg ataaagatgg tatttttttg tctgttctat ctttgaagag acttcttctg
     9001 taaagggaac tcgtcttcct gtcactagta atcggtgagt gttcacggtg taaggtgtac
     9061 aagtgataag tgtgactaga tcctgctttt ctctaattgc taatgtattt gtttttgttg
     9121 gaaggacaac aattttctca acgacttcat aagcgagcat ccgaccatac acttcaagat
     9181 agaatcggtc atcttgctcc atttcttcaa gatccgtaaa gagttttttc tcagcaatac
     9241 cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc agttggaaaa gaggtgcctt
     9301 gcaggactgt cgctcctcta tctaacaaaa gatcattcgt ttcatcaaat aaaggcaagc
     9361 taacattgat tttcggaata tagatggctc cgatcatatg ttgttcgtaa tattcttttc
     9421 ctggattttg gacatttcta accgcttgct caaaaggatc tttgacttgt cccattcctg
     9481 ggattactga tgtatgagca agcgtctgat ttttcttttc atgcactgct aattgttgct
     9541 catgtttttc ttctttatcg cgttcaagct gcttttgata attctcgatc cttctttgat
     9601 ccaaataatt gctgagagca tctgctaaaa atggatatgt aaaaactgca gctccggcaa
     9661 gaaacatcac tgccataaat aattgtaata aaagactgat ttgggtttga ggtctattac
     9721 ctattttctt tttcattttc ttcaccttct tatttaaaaa aactgcagca acctttataa
     9781 aaccgattgc tgcagcttat ttttgttgct tatacttcag acttcatttt agatttacgg
     9841 taccagctgt acgctccaat catcaaactt attccgatta gtaagaatgc caagagcccg
     9901 ttcccaccag tagcaggaag tgacccgcgt ttcatattcg gtactctttc tggccctgga
     9961 ttttctactc ctacgatctg aatttcttca ttatagcttc cataagagat gataaattca
    10021 gtaaaggctg cctcacctgt tggaagtaca taaccttctg gcgccatggt ctctttcatt
    10081 tgataggttc cataatctaa cccttgaacc tcaaagtatc catttccatc tgcgacaaat
    10141 tctgttgctt gtccgcttgt tgcccactca attctatctg ctcctgattc ataaacagct
    10201 aaaggcgact cccctttata aaatattgcg tattcagaac ggtcttcatt ccacaattcg
    10261 aatcttgcat ctttaagcaa ttcactgctt gatgcatcat atttttcaaa ctttcttcca
    10321 cctgttgtga ctttctctgg cggctctact ccttcttcat aatcaaattc atcatttcgt
    10381 ccgatttcaa taaccgctgt gttgttatgg aaatcatccg cttctagttc tgtggaaacc
    10441 ttcaaattat acgtgatcgt taaatctcct ccacgtgcat ctgctaatct acctaaagta
    10501 tctactttgg ctactccatt ttcttctgtc aaaatcgtca cgacaaaccc gttgcttact
    10561 acttctacta tataatcttg tccttttgtt aaaatcgtct cgccggctcg cacttctatt
    10621 tcctgatcat aataagttag accaactgta ggcgtatctg tgattttgta atttttgata
    10681 acagcgcttc cgttttcaag gacagaatcg atgaaataag gaataggtgc tgtgatcgtg
    10741 taagaagccg tttttcctct ttctagatca atataagaaa tcgtttcttg ttctccagct
    10801 tcattaataa tcgttacttg tctaaggtcg ctttcctcta cattcatctg tttatctgct
    10861 tctctgattt cgtttttcgg atacaaatgc acatctgtat tgtatgtgtc tttccatgta
    10921 gaaccatccc acacgccatc caccatatca ggcatcatca ctggcatcat aacaaccatt
    10981 ggagacgcga ttctggaaat atgagctggc gaataagttt ctaagaaaag atagacttta
    11041 tctctagaag aaggtgcttc ttccgtcact tggacggtag agaataccgc ttctccagct
    11101 tcattcgttg tttgtttgtc aatacttgtc agtcgatatt ttaaaaacca actttctgtg
    11161 tgccgttttt gaatccattc aatcgcttcg gcagaattaa gaccgtcttc tcgttccggt
    11221 gttaaaggat tatctcgaat caaatcgtaa tatacttccg ttacgtcaaa caaatcaaaa
    11281 tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa ccaatccgct attcggtcgt
    11341 tcttccggaa cagatgagaa accttttttg tgtaatgtaa tcgtcacttc acttggtttt
    11401 tgtgcgactt cttcggcttt gactagtttt ccgttcccga aaatccctat tcccgcagta
    11461 acgagtaaca gtacactcat tcccgtgaca atcatttttt tattcatttt tttctccccg
    11521 ttcttttttt attaataaaa cagcacctaa caataaaaga attcctagta aagaaatcaa
    11581 cgttcttgct tcattcgttt gcgggaatac ggaccatttt cctgattgac ctggattgct
    11641 tggaccactc ggctctttcg gtttgtccgt tgtttctttt acggtctcgt tataaacacg
    11701 tggtgttgcg gattcatagg cagagactgg aactttcccg gattctaatt cagccatctc
    11761 ctgtccgtta actactacat attgtggttg gccttttgca tccacccaaa attcagggat
    11821 gatgacctcg attctcttac ttgcttcatc catctcgtaa ccttccacac cttttaattc
    11881 ttcaaaataa taggtgccag aaggcaagaa acgttgtcct gtcgtaacaa ggccctcttt
    11941 atccgaaatg aatttgctta catttttgtc attcaaagga tcattgtctg ctggtttgat
    12001 ccattgattt ttcaagtcat tagcaggagc catatctaaa taatatttcc ccccttctat
    12061 catctgatat agagcaaaaa cagcaccttc taatggtttt cctttttcag ttgttcctct
    12121 ttgacgtcca tacttgaaga agtatggatc tcttagataa ccgacatttt taggatagat
    12181 atgaatctct tgcagttcct cggttgattc tgtcgggtga taaattggca aaatagcagc
    12241 aattgggata gctgtctgat cgatatcaac atccaatcca acttctggat caatcgattc
    12301 ttcgaaaatc aaataaactg catcccgatc ttggtttttc ttaggtgctt tgatacgtgc
    12361 aatcccgtct cctaatccgc caaccgtatc tttcccagtt gttaccgaag tccctatttg
    12421 gacaagcctt tcttctgtga ttagctgacg gatattgttt ctgttcgttt cagcgatttg
    12481 ctggacgaat tctttaggag tcatgttttc agcagctttt ttttcatgat aataatcggt
    12541 catgtcgtaa atggcaaatg tcgctccatt tagtggcacc gtctgtgaaa tgatatctgc
    12601 tgtttcccct gcttcctcag agatagccaa gccatcattt tcatggtatt caaattgatc
    12661 gttataatcg atatctcgaa tcatccgttt atgcaacacg aattccacga catcttcccc
    12721 tgattcttct gccgacatcg catgtggaag aaggactgct gcaagcggta gtaaaacaag
    12781 taattgaagc atcaaacaca acagcttttt cattaccttg ctccctcctt tttagattgt
    12841 ctgacaaaca aaccataggc tgctcctgca agcgccatga agaatgcagc aagcaggata
    12901 tagaataatc gtccggatcc tcctgtagca ggcaacggat tttcaggatc gttcccgaca
    12961 ctaagctgta atctgattcg atttgtctcc aaaagttcat attctaactc ttgctgtaga
    13021 tcaggcgtct ctgcttcttc gtttggataa tacgaaatct caacttttag tgtacccgct
    13081 tcttttcctg gaactacccg atcattttct atgtcaggtt cttctcgata actaatttct
    13141 aaaataaagt aacctggaag ttttctatag ctgtcaggtg acttcacttc cttgatccga
    13201 tagacccctt ccaacaagtc tgaagcattg aatagggaag aatcgtttgg atctggtgcc
    13261 aagcctccct cgatgatttc atactctcct gcactatttt gtttctcaat tgaaaattct
    13321 gcgccttcaa gacgtcggct attcatactg tcaattttca aaagttccaa atcgattggc
    13381 gctagttcat cttctaaggt tacttgtagt acaccatcga tgatttcata gccttccggc
    13441 aagggagcag tttcgtcttc gatgaccgta tcttcaatga taaacttacc ttcttcattc
    13501 agtgcaaact ggaacacttg atcagatagc cgatacccat ttggtgcgac agattccgag
    13561 acctcatatt caccaataga cagatgcaga ggttggtcgt aatttaaatc gacatacttt
    13621 tctactccta cggcacgaga atagaagtat ccgatcccag gcaaacgttt cgattcttct
    13681 ggttctaacg gattaccagt gtatgctcga tacagcggga aggaagaatc ccccactttc
    13741 ctgatatcga aacctgcacc atctaatgta tggccttcat ggtctaattt tctaactcgg
    13801 atctcccgat aatcaaacgt attgtaagca gtgaatgcta cccataaacg atcctcttca
    13861 tctgttgtat acgtgagata ttgccaattt tcttcacctg tttgcttgag ttggatagtc
    13921 cctatagcat tatcgtaaag tcgcaagatc catgtgtcat tttcatcagg ttctttgaaa
    13981 ccttctgggt tctggctctc aatgattttg tattctgcat agttggtagt ttcttcacta
    14041 tccttataaa caaatgaatc tggctctccg attggatgaa taactttctc accatccacg
    14101 attacatatt tcccgtcttc atctactgaa tagaacgtga gtctcccttt ctcttcctct
    14161 acttcgttca caccagaaag ccctttcgct actggatatc cgatttccag tccgttacca
    14221 tctgtttcat aaagttcaaa ctctgcgcca ttgattggca tattactgcc ttcaattagt
    14281 ttattgatat tcatgtggat tggcttgaat tcatttcgca tttccagttt gacatacaac
    14341 tcatcatact ctgtgccttc tatgttaggc tcttgtacaa cagaaagctt gtgatattct
    14401 ccttcataat aggaattcgg atctttacct tccggtccgc cgactctcga accagatgta
    14461 tattcatcta caagctcgta gtgacctaac atctcatctt cgttggctgc atattttttg
    14521 atttggaatt gatacgtctc atctactaac tgataacgtg gtggtccagc gatctctctg
    14581 atccaaaata tgtcgctgac tgctccagtt tcatctccta aaatcggcat cttcgtttca
    14641 aagatcgcta ttccgtccgt ggtagtttta cttgtttcga ttggttcggc ttccccactc
    14701 tctggatttt ggtcatataa tgcaaattcg actccagcca acggctcgcc attttgaccc
    14761 ttcttgctga cttggaaatt acggccttta tgttcgtaac gaatggaaga agatgggaaa
    14821 tacatccgtc cagtagtgat atcattctca taatcctcca ggttctgaat ataggccata
    14881 ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat caagttttac ttggtacttg
    14941 atcctgacca tttccccttc acctaaagaa atcggagaaa atttataagc ttggttttcc
    15001 cgtccgacat acgttgtcgg agtcgccact acttctggtg gggcttgtcc atgacgttct
    15061 tcatcccata tttctggctc ttggtacttc atgccgtttt ctcctggatc tagatggtaa
    15121 aactgaatat ctgccgatct gccagctcca aatggtgaga cattaaaacc agtttcatca
    15181 taaagtgtgg cttgctggat cgttctcttg aaggtagaag agatatcctc caaggctgta
    15241 acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg ttacgccatc ttcttctaaa
    15301 tcagcaatat ttttcaatac atttcgtcca atagcatcta gtcgttgtct cccgctaacg
    15361 tttccatcga ttcctaaacc aatggagaag atacttggcg ggttctcttg atacttgttt
    15421 ttcagccaat gatgataagc aactgtgttg atcgcagatg acttttcttg cgaagtagga
    15481 ctgccattcg tattgttatt agaagaacca tcaccaaccc agtgagtcgc tttattatct
    15541 ccttctttta cctctccata acgatagtgt acatttactt catcttctgg acggttaaag
    15601 tcagatgcat aggtaactgg atgagtaaac cctgcattag aagtattccc agaaaactcc
    15661 tcaaaatttc tgaacaaaac gtcattatct tcagcaatct tatcttccat cacagtccag
    15721 ttgctccaag ctccattagt gctcgtacga taacttgact gaatcggtgc ataactgaag
    15781 gtcggtgtcc catctcccac aacgattagg attttttctg gatttctatt ttctccgcca
    15841 ttgtctttat acaatgtttc atagccattt ttaagcccta gtgtaagcgg agttcctccg
    15901 attggtgtac gatttaatgc tgtattctca cttcccgtca aacgattgat atcatttgtc
    15961 aatggaaaga tgtccgaact gatttgctca tggttattga tagctgtact attatggtaa
    16021 aaattcacca taccgattcg aatatccaga ctcgggtcag agagcagatc tttactcatt
    16081 tcgtttactg cctcaatcaa cgcagcattt ttagtttgac tattggtccc agcggttaat
    16141 tcactcatac tcgctgactt atctagaaca aaaacgatat cgattggact gatttccttt
    16201 ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg ttgttgcatc ttcaggatgt
    16261 agatcagatg ctgtgctgct tggcagaaca ggttctaccc attttttgat gtacgccccg
    16321 ttataatcca agtaactgtt ttcaaaattc cgttcatttc cccaaagctg aacggtttga
    16381 gctggaacgt ttctatcttc ttcgcctgca gttgatccat tattagtagc atagtcgtaa
    16441 tttcgccagt tcgctccccc gtttgtataa tcactatcat cgatcaagtt ctctccgtca
    16501 tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt gtgtccaatt tcctaacact
    16561 tttgtatagt cattcgttgt aaacttcggg tagcgatgct gttcgttttc aacttctccg
    16621 ctaggataga tatattcata ggggtcttgc gtagctgttg gcccaagacg ctccaaaata
    16681 ccgttgaata actggcgttg aatgcgctgg agtgcttgac cgctgggtac gattggttcg
    16741 tccagctttt ccatctcatt ttcttcgcta cgttgaattt cagcggaatc ttctgtatca
    16801 gtttcatttt cttgtaattc ttggttatct attctttcat caaccaccac ttgaagaggt
    16861 agttaccctt cgttcacatc ggggatcttt tctgtttcaa aaatcaatgt gccggtttct
    16921 gcttcttttg aataaacttt tccgacatac cattcttgac catcgatcgt ctcgactttt
    16981 aggtcttctc catcttcatc ataggaggat agacctgttc cacgaacgtt ttttacggta
    17041 ccaatcccgg cggctgccgc atcgattgct aaacgtattc taccttcatt ggcactttcc
    17101 tttttttcaa aagccagatg ccattcaagg gtttcttctt tttcaatata cttcccttca
    17161 atttttgcat agtcctcatc taagaatacc tcatcctcct ttgtttctgt tgactcagca
    17221 gctacatcca gggtcgtcat atgatagact gactggaaat atggtccaaa tagcaaactg
    17281 acagttacta actgcaaaag cttcaatttt aaccctttca catccttata ctcctttcaa
    17341 aaatatttgt cttctttcac aacaatcaac aaaacaatgc ccatctaatt ataatttaat
    17401 ttaattttaa ttttaaatca aatgatatcc tttttagtta aaaaacacca attatacaac
    17461 atttattatt aaaattaaat tataaaattt tataacgttc tcatttaaca aaacttaagt
    17521 tattaagaca aatgaataaa caactacgct ttcctcgaaa caaaaaaata aagcacaaat
    17581 gcgcatttac gcactcatgc tttattcgta caattaattc agataacggc agcgcatagg
    17641 atgccaataa cacttcctaa aatagctcct cctaaaatgt cagtaggata atgtaaaagt
    17701 aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa aggctaatat tcctgcaact
    17761 ggacttaatt cgaacaacgc agctactgat aaaaaggcta gcgctgtatg tcctgaagga
    17821 aaagaagaag tcttcggttt acttgtgtaa aggacaagcc gacgattttt ttcataaggt
    17881 cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa caaaaaaata gatcaaactg
    17941 ccgataagaa tggctagacc atattctcta gtatcaggta tcaagactaa aacagcagca
    18001 aggagcaacc aaataaaacc accattagag atattactga taaatttcaa aaaattgatt
    18061 tttcgactat ccgttttccg ttgcttggcg aacttgttga taatactatc atcgatttta
    18121 gcaatcaaac gcaacatgtt attcctcctt tttaagaacc ttttcttgcg tacgatggat
    18181 aaaaagcagg acttgaaaat aaaacaagaa actgaaacct aataaagcaa aatagattgg
    18241 tcctgcaaac cagaatgcga caagaaggat caacgtgcct gccaaataga aaagcgcgct
    18301 tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa gaagcttctt tttgttccag
    18361 ttgaagcaag attttgtaac taaagagaag cgcgcttaat agaagcaaaa acaacgtagt
    18421 atacatcaat gtatagccaa ttcccccacc ccaatagcgg aaaatctgat aatcgatcaa
    18481 tagaagaaga aaataaacac ttgctaaact atcttgcttt ccataaagag agaagttctc
    18541 acgaaacttt ttccagaata gtgaaccgag ctttttctct tcattttttt tgtaatgata
    18601 aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca agacgcacag
    18661 gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt ctaagaaata
    18721 atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc tgtcaaatcc
    18781 atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg gatcaagaca
    18841 agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg aaaaacttgc
    18901 aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg acctaagaaa
    18961 tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg tttcgtcaat
    19021 ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc agcagcttcg
    19081 atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt gaacgcattg
    19141 ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat cttggagaat
    19201 aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga aagaaccaaa
    19261 gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc aataaaagaa
    19321 tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt tcttgcatag
    19381 cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat tttttctggg
    19441 aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt aaacaccatc
    19501 caaagcaatg gaatgatcaa aatgattcct attactgtca tgaggacata acttagtatt
    19561 tttttgtttg ccgattcaca ctggtctttt tgaaataatc catcatttgc cgtcacctcc
    19621 agaataatag acccacttag gtgcaagctt caaattgaca aacgtcagaa ccaacacgat
    19681 cagaaacagg atcattgaca tagccatcgc ataacccata tcaaaagcta cgaacgcctt
    19741 gttccacata tgcagattat aaaaaagcaa agaatttgca ggtccgcctg ttccattttt
    19801 cgtcatgaca taagcttctt gaaaaatttg gaaagaaccg atcgtagagg tgatcacatc
    19861 gtaaaaaata ataggagtga ttaatggcaa agtaatactt ttgaactttc cccaaacgcc
    19921 tgctccatcg atttctgctg attcatataa agatctagga acattttgta atgtagctaa
    19981 gtaaagtagc attgctcctc cagaactcca tattttcatc aaaatcaacg caggtttcgt
    20041 ccattgtgga tcaaacagcc aagctggtcc agggatatgg aaccagccca gaaatgtgtt
    20101 gatcaatcct gcagatggag aaagcagctg catccataag atgtagactg ctactcctga
    20161 taagactgcc ggtaaataga aagctgtacg gaatacactg attcctttga ttttttgatt
    20221 gagtactgtg gctaaaaaca cactgaaaat cgtcgtcagc ggtacattga agatgacgaa
    20281 ataaacggtg ttgtataacg atttccaaaa taaatcatcg tgaacgaaca ttcttttgaa
    20341 atttgctaga ccaatgaaat tcatcttgga tgtcaaatta taatcggtga aactaccaat
    20401 aactgaaaaa agcataggcc ctagcataaa taccaagaac ccgataataa aaggcgagat
    20461 aaataaatag gcccatcttg cttctttttt ctttcggata gataaacgct tcaaattttc
    20521 ttgcctcctc tgtacctaga cagtcaaaaa tggattattg attttgcttg actaagtttt
    20581 ctactgcttt ttgtgcatct gccaatcctt cttttggtga ttttgttcct aaaagaatct
    20641 ggtcaatctg ttcattcact aatgtcgtgt aatccggagc atctaaagga actggtgtga
    20701 tcaccgtatt tttaaagttt tcgtccgcca tttgatagat catttttcct ttatccgtta
    20761 ggttgtcatt attcactaaa ttctcattgg ctgtcttgct cgccatgata tcaaaacttt
    20821 tttcgccgaa tttttcttgt acttctggag aagaaagata tttgatgaat tcataagagg
    20881 cctcaggatc ttttgctccg tatggtactt ccaagacgaa tccgccaccc cacgaccaat
    20941 ggcctgaacc ttcttctttt tctgggagct gcatcacgcc aaaatcaaaa tcatccggtg
    21001 cattttccat caaactagag taataattga tattttgggc acgcatggct actaatccgg
    21061 aaataaatgg atcagataca ccagacccaa attcggcttc taatttattg atcgtatttt
    21121 tcccataata atcttgccag tcttggatcc actccaaggc ttccacttta tttttagtat
    21181 caatcttgac tttttcatct ttgtcaaacc aactagtccc gttatctgcg tttagcgccc
    21241 atacatcagc ccctaaattc cataatggat aaaaaccgat gcgtgtaaat tcttttcctt
    21301 cttttttatc tagtttacgt gctgtttctt ctaattcatc ccaagttgtc ggtagatctt
    21361 gttcactgat tccagcttct ttaaatatct ttttattata aaaaatgact tgtgtatctg
    21421 tattgaatgg gatcgcataa ggatcacctt catattctac tgtatcccaa agctgcggat
    21481 aaaaatcctt agaagtgtct ttttctacat atttggagag attagttgct tgctgtgctt
    21541 ctgctctttg ggcaacggaa tttatgtcct gtacaataac atcgggtgca tttccagctg
    21601 ttattgcaga taaagattta gtccagatgt caccccatgg ctgatactta taatttacat
    21661 ggatctcgtt ttgtgattga ttgaaatcct caatgatttc ttcgatagct tcttttcgtg
    21721 caccggatcc ccaaaaggac cagaaatcaa tattttttct tccatcactt gttttttcag
    21781 aagaatttgc tgcacttgaa ctggcatcgc ttgcattact tgatccgcat cctcctaata
    21841 aaaacaatgc tgaacttgcc agcaaccctg taaacaataa ttttttcata ttttacctcc
    21901 aaattttaat gtaaccgatg ccggttttaa tataacgata tcattttgtt ctgaaaataa
    21961 caatttataa ggtatgattc taaaaaaatt attaatgata ccaatacttt atataattcg
    22021 cgagcgcttt ctttgaattt tttaatcttt cgtagcagaa tgagaatgta ttaaaaatag
    22081 attaggagga attttgatgt ttagacacca gaaagaattg cagtttgaag caaaaccaga
    22141 tcgtccgaat ccattaatcg cgaaatatct gcaggagctg atcggcggcc aatatggcga
    22201 gatgagtgta gctatgcagt acctgttcca aggctggagc tgccgaggag aagaaaaata
    22261 taaagatatg ctgatggaca ttgcaacaga agaaatcggc cacgtggaaa tgttggcgac
    22321 aatggttgct cgtttattag agggtgcgcc tgttgaagat caagaagcag cgatgcaaaa
    22381 agatccaaca atcggtgcca ttatctctgg aatgaatcca cagcacgcga tcgtagctgg
    22441 cttaggacct cgtcctgctg acagcgtggg caatccatgg agcggtgcct atatcattgc
    22501 gagtggcaac ttgttagcag actttagagc taatttaaat gccgaatcac aaggaagact
    22561 tcaagtagca cgtatttacg aaatgatcga tgatcgtggc gtaaaagata tgctgtctgt
    22621 tctccttgca agagatagct atcatcaaaa tctatgggaa tgtgcagtga aagaattaga
    22681 agaaaaagaa ggttcaatct tagtcccaag tactttcccg cgtgacaaag aacgaaaaga
    22741 cattgcttat gacttctata acttctcaga gggagaccaa agtagtgaag gttcttgggc
    22801 tagtggcaaa gcactggatg gcgaaggcga atatcaatgg ttgaaagaac ctaagatcga
    22861 aggcgctgca ccaaaattag aaccagtaac accaaaaatg tatgggacac ctccattcaa
    22921 gaaataaata tctacccgta gaaacacccg ttttttgttc cacacgcttt gtgagcaaaa
    22981 ctgactaaag tcattaatca taaccaccct tcaaaagggt ggttttctct agagctataa
    23041 gctcaaatca ccggccagcg cctaaagacg ctggctttca tattttatgt tcaagccata
    23101 ttgcctttgc cagttttgcc cacctcttaa aaggtttatg tactactcct tagcccttaa
    23161 aaggatcgtc ttactctcgt acactcagct tatctaacgc actatcatgt ttttcttgtt
    23221 cttgaatgta ctttttgatg gtcgcttcat tttgtcctac ttttgtcaca tagtatcctt
    23281 ccacccaaaa gtgtcgatac ccaaatttat attttagatt tgcacaatct cttgatgatc
    23341 tcctgaatat tggctcgata ttgattataa attatttttc ttctatactt tggtgtgaat
    23401 acaatgtggt atttacacat ctactttgta cgggctaaac tatttccctt tattgctcca
    23461 cccgcatagc aagtggtttt gtatttcaat cgctcggcgc gctcaattag gtctaaagac
    23521 aataaatcta aaaacccgct gcttcactga cagaaacaac gggttttcag aaaaaaatga
    23581 aatgaaaaag atctcttacg gcaatctatt ttattttttt gattttttat aaatggaaaa
    23641 tgcttcaaga agtccaagaa ataaagccgt ataaaaacaa gaacctatga aatctttatt
    23701 tattagatat tcaacagaaa tcccgataac actagccaac aatatattta tcagaagtgt
    23761 aaaataagga tgctttttat agaatagaac tagtgttgtt ttcatagact ctcctttctt
    23821 catatttcca attaatcaca aattcacggc attactagaa aaattaaagg ggcgcagaag
    23881 tggaaaaata ctcttcgact ttccctacaa accaggccat cttgtgttta ataactatac
    23941 caaatctaag gtacctctaa atctaaattc catttgtggc ggtgacctcc aatttttaac
    24001 tcttctatct cccatccagt atacataacc ttgatacttc gaccacttat atatcgaaca
    24061 cacaaatacc tatccattgc aggagtacta cctaacttag ttgctacttg catctaaata
    24121 acagctgaaa agataggact tactcttagt aaagtatctg aactattaat ttttaaacta
    24181 tcatttttat cctcaactac atgcacatct gttgcaactt cactttctgt ctcatccaga
    24241 taaatgatta aggaatcggg aattgcaata tcttcagctg acgctggaat agttgagcta
    24301 aattagtcat catatcttta ttataaaata accaacagca aaaaatacaa tgaaaaacaa
    24361 acttggaact aatgtatcat taataaatgt tgaaaaattc agcaactcat tactgtatga
    24421 ttttagcagc ccaaccaaca tataccctat aaaagctaat aagatggtta ggaatccttt
    24481 agaattttta ctcattcata gacacttcct taaataactt ttattataga aggtattgta
    24541 aataataata ttgacaaaaa gtgactcaaa ataaaaaaaa ccttacatta tactggaatg
    24601 aactgtattg atagtgtacc taaaagctct actgacataa gatcgtgtaa tcggtaaagt
    24661 aattttatta attatacggg atgtaatcaa ttagagaata aactaatcat aaaaggtact
    24721 tttacattca taattgtcgc tttgagattc tactttggtt cactatctga cccaattgca
    24781 cattttcgat cattacaagt attggtgtcg ttagaacaac taaataaaaa ctagcggtag
    24841 atttttcaga atctatgcta atttctgtat ggatgttcca gtgatggcaa atttctcttt
    24901 tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta ttcagttaaa ctatagaaat
    24961 tctttcctag acagatcttg atcccaaatt ttacgttttt gagcatcgat atagtttaaa
    25021 atgacattac tcatatctaa agagtttaga ttttttagga ctaaaactgt cagttgttct
    25081 ttttcttctt tctgttaaag tattgtcgcg acttttcgta tacgttaaga tatgtcgtga
    25141 aaaaacaagt tttgtgacac aaaaaaacga atgacttgag gaaatcacct caatcattcg
    25201 tttaaaatgc tatatattct ttagtttaag atacggaaat taatgccatc gaccacacta
    25261 gtagaatgaa cgacatttac ttggcctttt tttaatttcg ttatgtttag catataaaaa
    25321 tattatagag tttacttaca atgcttaaaa catatactgt ataatctgat caattttagc
    25381 catgtcttgc aatggtaagt tttctatttt tttgagtttt cgttccttaa aatcaagaga
    25441 ttttaattgt gtaataatta cttgtccttt tatatctagc tcttcaggta atgagtaacg
    25501 tgtcggtaaa ttctttatag tggaagtaat tggacatatg accgaaaaca ttgttttacg
    25561 gttaaattca taacgagaga cgactagtcc tggtcgtctt ttttgaattt cttttcctac
    25621 ggagggatca aaatcaatcc atacaatatc acctttttta ggaatataaa ttctttcttc
    25681 tgacactata aaatttctct cccttcagga gttaaatctt cccattcgtc tttctcataa
    25741 tactcggcct cttccccacc actaaatgga tcttcaattt ttggaactaa cgtaattgtt
    25801 ccatcatcag aatacacaac gatatattct tggttttctg aaggtttctt tccgttatca
    25861 gaaggtaaag ttaccactac agaacttcct tgtaatctag attttgtaac aagcattatc
    25921 atcactccct taataaaagt atactatata acactaagta acgcaaaatt cccctttgta
    25981 aactatctag aaaattgttt tgtttaaagt atatcttatt ggatataaat agaaataaat
    26041 tgtagccagt tcattttttc tttaactaaa aaaataatcc aagaaacatt gaacctatgg
    26101 atccaaaagc tacccaaata ctatagctaa ccccccgcta ccttcaattt tgcagataaa
    26161 acgctttggg agaaaatttc aaagacaaca ggccatccta tcatgcccga ttataaacaa
    26221 aaagctggct attccttttg ctctgacaaa tttcaattgg gaaagttcac gaaactagca
    26281 ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc ttcaaataga aacattggtt
    26341 caacgaattt caactagaca agactttgct attgtagaac gtgagatcgt agctattttt
    26401 gtagaagaag ggattttaca tgatccaact catatcaatg tggataaatg gcaacctctt
    26461 atctataaat tcagagaata tacaactgcc aaccagtcgt taactttgaa ttttagattc
    26521 caagaattca aagagtcata atgataaaca tcgttaggta taaataaaaa agactcgcag
    26581 cacgagtaaa aaacaaatag aagctataca ttagaaaggt cgactgtgac cctatagatg
    26641 cagtatatgc agcttatgag catttcttct gttctctttg tttttgggat ctgtgctgaa
    26701 gtctttttcg ttttttgccg ctctttcttt gaaaaatcga ttatgttata ccttctccat
    26761 tactaattca ggcatctgct gagtaatgat ttttcgcgca aaagaaatat ctgttaccca
    26821 aggttcgatt tgttcttcag tcaagacaag cggcattcgg tcatggattg gtgcgactgt
    26881 gcggttaggt tcagtagtca ttatgattga ttctgccatt ttttcatcct gatggctacg
    26941 ataaaaaccg ccaatataca ctattttttc cttatttgaa aacataaatt tgtctttttc
    27001 ttcattccac tcattgggaa aacacaacga ttttgcaaaa atggttgatg aaaggtcttt
    27061 ttttcttcta ctgtctctgc acgggcatta atcatcaatt gtcctttttt aaatccggta
    27121 aatccccact tcatgatttc cggagtgagg atgttttctt cctcttttct taacgtcaca
    27181 acaagatttg acggaaaaac ttctccagct gctaattctg ctgcctgctt cggttgtttc
    27241 gtctgggctt ctcgataata attctgaagg agctctgact ctagatcaaa aaaataacga
    27301 ccacacatcc taatccctcc caatcttttt ttctatcata cactgcaaca agaagttcgc
    27361 accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct
    27421 ccagtgaatg gtattctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
    27481 gagaagctgc tttttgaaat tgttccttgt gactcggagt gaaacggcag gcttacgacc
    27541 tctgggtaaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
    27601 gtagaaagct attttcacaa aatttttctt aatactcgaa actaagcgac t
//
LOCUS       DACEJZ010000032        27560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEJZ010000032 DACEJZ010000000
VERSION     DACEJZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.032"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..873)
                     /locus_tag="IW656_001256"
     CDS             complement(<1..873)
                     /locus_tag="IW656_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0836973.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
     gene            1139..1483
                     /locus_tag="IW656_001257"
     CDS             1139..1483
                     /locus_tag="IW656_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0836974.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1484..1903
                     /locus_tag="IW656_001258"
                     /pseudo
     CDS             1484..1903
                     /locus_tag="IW656_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1995..2345)
                     /locus_tag="IW656_001259"
     CDS             complement(1995..2345)
                     /locus_tag="IW656_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0836975.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2311..3642)
                     /locus_tag="IW656_001260"
     CDS             complement(2311..3642)
                     /locus_tag="IW656_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0836976.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3898..4464
                     /locus_tag="IW656_001261"
     CDS             3898..4464
                     /locus_tag="IW656_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0836977.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4609..4696
                     /locus_tag="IW656_001262"
     tRNA            4609..4696
                     /locus_tag="IW656_001262"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4641..4643,aa:Ser,seq:cga)
     gene            5030..6004
                     /locus_tag="IW656_001263"
     CDS             5030..6004
                     /locus_tag="IW656_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0836978.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6329..8224
                     /gene="asnB"
                     /locus_tag="IW656_001264"
     CDS             6329..8224
                     /gene="asnB"
                     /locus_tag="IW656_001264"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0836979.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8419..8865)
                     /locus_tag="IW656_001265"
     CDS             complement(8419..8865)
                     /locus_tag="IW656_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0836980.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            9093..9242
                     /gene="rpmG"
                     /locus_tag="IW656_001266"
     CDS             9093..9242
                     /gene="rpmG"
                     /locus_tag="IW656_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0836981.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9330..9848
                     /locus_tag="IW656_001267"
     CDS             9330..9848
                     /locus_tag="IW656_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0836982.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9925..10881)
                     /locus_tag="IW656_001268"
     CDS             complement(9925..10881)
                     /locus_tag="IW656_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0836983.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            11047..11853
                     /locus_tag="IW656_001269"
     CDS             11047..11853
                     /locus_tag="IW656_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0836984.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11850..12839
                     /locus_tag="IW656_001270"
     CDS             11850..12839
                     /locus_tag="IW656_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0836985.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12836..13840
                     /locus_tag="IW656_001271"
     CDS             12836..13840
                     /locus_tag="IW656_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0836986.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13972..14514
                     /locus_tag="IW656_001272"
     CDS             13972..14514
                     /locus_tag="IW656_001272"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0836987.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14534..15232
                     /locus_tag="IW656_001273"
     CDS             14534..15232
                     /locus_tag="IW656_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0836988.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15253..15465
                     /locus_tag="IW656_001274"
     CDS             15253..15465
                     /locus_tag="IW656_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0836989.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15465..16427
                     /locus_tag="IW656_001275"
     CDS             15465..16427
                     /locus_tag="IW656_001275"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0836990.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16445..16840
                     /locus_tag="IW656_001276"
     CDS             16445..16840
                     /locus_tag="IW656_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0836991.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            17003..17368
                     /locus_tag="IW656_001277"
     CDS             17003..17368
                     /locus_tag="IW656_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0836992.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17365..19176
                     /locus_tag="IW656_001278"
     CDS             17365..19176
                     /locus_tag="IW656_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0836993.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19238..19405)
                     /locus_tag="IW656_001279"
     CDS             complement(19238..19405)
                     /locus_tag="IW656_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0836994.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19653..20642
                     /gene="galE"
                     /locus_tag="IW656_001280"
     CDS             19653..20642
                     /gene="galE"
                     /locus_tag="IW656_001280"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0836995.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20654..22138
                     /gene="galT"
                     /locus_tag="IW656_001281"
     CDS             20654..22138
                     /gene="galT"
                     /locus_tag="IW656_001281"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0836996.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22162..23142
                     /locus_tag="IW656_001282"
     CDS             22162..23142
                     /locus_tag="IW656_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0836997.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23231..24055
                     /gene="tsaB"
                     /locus_tag="IW656_001283"
     CDS             23231..24055
                     /gene="tsaB"
                     /locus_tag="IW656_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0836998.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            24039..24581
                     /gene="rimI"
                     /locus_tag="IW656_001284"
     CDS             24039..24581
                     /gene="rimI"
                     /locus_tag="IW656_001284"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0836999.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24588..25052
                     /gene="rimI"
                     /locus_tag="IW656_001285"
     CDS             24588..25052
                     /gene="rimI"
                     /locus_tag="IW656_001285"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0837000.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            25049..26065
                     /gene="tsaD"
                     /locus_tag="IW656_001286"
     CDS             25049..26065
                     /gene="tsaD"
                     /locus_tag="IW656_001286"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0837001.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26674..27375)
                     /locus_tag="IW656_001287"
     CDS             complement(26674..27375)
                     /locus_tag="IW656_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0837002.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8889 a         4725 c         5707 g         8239 t
ORIGIN      
        1 attatttcgt ttagatgcga cagattgaat aaaaaattca tctactgaat tgaaatccat
       61 agatttcaat aaaccatctc tacgtaacaa tccgttagaa ttctcgttta ggcctctttg
      121 agacggcgtt cctggatcag cgaaataaat ggcaatatca ttgacattac tgatctgttt
      181 ccaatttgaa aattcctttc cacaatcaaa agtgatggat ttgaataggt ttttcggtac
      241 agattcaaac cattgattta attttttttc aatatcaatc gcttgtctac cacacggttt
      301 caatgtgatg ataacttttg ataatcgttc aactaaggta attacagcac ttttatgttt
      361 cagacctacg atagtgtcac cttcaaggtg accaaactca tttgagaatt ggctataatc
      421 cttttcacgt tcatgaatag agcggcggaa agtttgtttt ccacgtcttt cttgatgtcc
      481 attcggttta cgcttgcctt tcatcggtaa gtcagaagaa tcaaatagcc cctttttgaa
      541 catacgataa atggtacgag cagaacaact aataggaaac gctgcacgac caacaatcac
      601 atcgggtgtc catccttgaa caacctttct ttgaatatat tctgattgtt cctcgggtaa
      661 aacaagcgga cgtctaccac agtttgattt gtttttctta tactgttgat aatattctaa
      721 ggctgatttt ccttgcttga agaacagata tactttatgg atcgtttgtc ttgaacgatt
      781 caagcaatgg gcagttttag caacagattg attcattttg aaataagact ctattattac
      841 aagttcatcc gttgtaagat gggtataggt catttgtact cactccttat aattctttgg
      901 tcggaactat tttgagtgta gcacaaatga cttttttagt tgtctagctt aattttacaa
      961 tcggcgaata aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta
     1021 agcattggac aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata
     1081 aagttgcctt agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat
     1141 ggcagaaaaa caaaccgcag gacgagatca attaggagat tttgcaccaa aatttgcaga
     1201 attgaatgat gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca
     1261 gcgtagtatc atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc
     1321 tcatctgaag attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac
     1381 ccatttagct ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga
     1441 aatctattta gaagaaaata gcgaagataa gaaagaggcg taaatgacga caaaacacga
     1501 agaaatacac aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt
     1561 atttaccggg taaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt
     1621 aggtaatgta acttttgaac caggttgtcg caatcactgg catgtccata aagacggcta
     1681 tcaaatctta cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag
     1741 acgattaatg gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc
     1801 gacaaaagac agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga
     1861 acctgtaaca gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc
     1921 tcctgaaagt agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata
     1981 aatagaaaat agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata
     2041 atcggctggg catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct
     2101 tcccgatcat gtcggggatt acctaatagt tcttgataat agatttccat actagggtcc
     2161 cgaggattgt tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta
     2221 ccctgtatct ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt
     2281 atttcatggg aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt
     2341 tccaagtgtc tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat
     2401 acctgcaacg aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt
     2461 gctgacgtta tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc
     2521 gtaaaaaccg cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt
     2581 actggtgatc tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg
     2641 aaaactgttt tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc
     2701 gatgacaggt ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc
     2761 aactaagcct atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt
     2821 caattttcca gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata
     2881 gaaaccaatt ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg
     2941 atattcattg aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc
     3001 agtaaaagtc atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg
     3061 atggaaaact aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc
     3121 taaattcccc aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact
     3181 aacgaccggt gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt
     3241 attgacaaat acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt
     3301 tttgccaaaa aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa
     3361 aatcaccttg tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat
     3421 gaatgcgctt ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt
     3481 aagaatagtt agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa
     3541 aagaaaaaaa acacctgtaa aaccaagata aaaatacttt cttttctgtg aaacgattcg
     3601 tttttgagga tgaatataaa taacgtcagt ttcgttttcc attacacacc ttctttttca
     3661 ttttctttaa ctataacttg aaatgaaaga tttggacaac tatcatttag gtcaaaacta
     3721 aaaatgtaag ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta
     3781 aagaagactg aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc
     3841 taaaaatagg gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg
     3901 agaagaaaag tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag
     3961 gaagggttta aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag
     4021 ccgatttatc tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc
     4081 tttgaacaac taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat
     4141 ctcggtttga actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac
     4201 attgatgaac atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa
     4261 atagttaaag aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat
     4321 actggtacat gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat
     4381 ccaacagaag aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta
     4441 gataatcctg aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt
     4501 ggctgcaaac tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt
     4561 agaaataggc ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca
     4621 gagtggtaat gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca
     4681 aatcctgtac cttccttata tcaacgatta gaggttgtga agaagctgtc ctgcatcaag
     4741 taataagaac ggccaaacaa aaatagcttt tcctattttt tcgtttgatt gggcttatta
     4801 ccgcagatgc aagcttttga acaccgtttg ttcggaataa ggggctgtga caagactttt
     4861 gtcacagccc cttattgttg tacttttaac ctacaatatt ttttgattcc ccctgagtac
     4921 tattttttat aaatgctagt aaatcaatct tctttggcct ttttttgata aaaaaaatac
     4981 tagatgtaag ataaaagtgt aaaaacgatt tgttttagga cgggaaacga tgtgtaccgc
     5041 aatcacttat gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta
     5101 taatgaagtg gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa
     5161 tttagataaa cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta
     5221 ttatgaggct acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc
     5281 tgattataaa gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg
     5341 gatactaggg caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct
     5401 agtcaatatc gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga
     5461 taaagaaaaa tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc
     5521 tgtcggtgtg ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta
     5581 tcgctcgcta tcaaatggaa caccagaaaa tcatttttca aaccagatca gcttgaatgt
     5641 ttatagtcgt gggatgggag gattgggctt acctggagat ctctcttctg tttctcgatt
     5701 tgtaaaagcc acatttacta aaatgaatgc tgcatcaggt gattcagaat ctgaaagtat
     5761 cagtcaattt tttcatatac taggctcagt cgagcaacaa aaaggagtat gcgatactgg
     5821 agaaagaaaa tacgaatata cgatttattc ttcttgctgt aacgttgata aagggattta
     5881 ttattatcgt acgtatgaag acagtcaaat tactgctgta aatatgaata aagaagactt
     5941 ggatcgtcat gaattggtca gttatccagt cataaaagag caacagataa actatgtcaa
     6001 ttaattaatg aatagatccg tttggtatca tattttctga tacagtcata attagacacg
     6061 atctttaggt cgtgtctttt tttgctggtt tttttggaaa tagcatccac taatattttt
     6121 ttaacacgaa gagaataagg tagcctcaaa aaagaaaaca tggtaaatta gatattggga
     6181 ctttattttc tgtaaaaaca gaaactttca tcttttgttt tcacgtgacg aaattgtaag
     6241 agagaaaata ttcgtccgct atttattcta tgataagatg catatgaatg aaataaacag
     6301 taattttata ttaggatgtg aaagttaaat gtgcggtatt gtaggttttg ttaacgacaa
     6361 ggacaacaaa aaaacaatta ttaatacaat gatggatcga atcgttcacc gtggacctaa
     6421 tagttcagga gaatatatcg ataaacatgt tgctttagga ttcagaagat taagtattat
     6481 tgacttagag ggcggtactc agcctattta caatgaagat cggacaaaga tcattatttt
     6541 taatggcgaa atctataatt accagccttt gagagaagaa ttgattgcag ctggccacgt
     6601 ttttcaaaca catgcagata cagaagtcct tttgcatggt tacgaagaat ggggaacaga
     6661 attattgcaa aagatccgcg gaatgtttgc ttttgctatt tgggataatg aaaagaatga
     6721 gttattcggt gcaagagacc actttgggat caaaccatat tattatgcag aaatgaacgg
     6781 aacatttatg tttggttcag aaatcaaaag ttttctgcct catcctgatt ttaacaaaga
     6841 attgaacaaa gaagcgttga aaccttatat gactttccaa tattctccat taaacggcga
     6901 aacattcttc aaagatgtct atcgtttgcc tgaaggccat tattatacgt ataaagatgg
     6961 aaaaatagac attcagcaat attgggatgc agattttgaa acaaaagaaa cgcattcccg
     7021 acaagaatgg atcgaaaaaa tcgacgaaac cgttcaagct tctattgaag cacatacggt
     7081 cagtgatgta gaagttggtt ctttcttatc cagcggggtt gattccagtt atgtaacctc
     7141 tgtattgaaa ccagatcatt ctttctcaat cgggtttgat gataaaacct ataatgaagc
     7201 gattgaagcc agaaaactaa ccgaactatt ggacttagat aatacagcag cagtgatcga
     7261 cggagacatg tcgttcaaag cattcccttt gatccagtat cacttagatg aacctgattc
     7321 aaatccttct tgcgtacctt tgtacttttt agcaaatctt gcttcacaaa gtgtgcgggt
     7381 agtccaatct ggagaagggg cggatgaact gtttgcggga taccaaacgt atggattcca
     7441 tacgaattca aaattcatcc gtgtgattgc tcaaggattg aaaaaattac caaaaggaac
     7501 acgctataat ttaggacgta aaatcggaaa aatgaaaaat ttccatggac ggatccattt
     7561 atatgaatca cttgctcctg caaaagaata ttttatcggt catgctcgag tgttcgaaga
     7621 atcagaagca tcagaagtgt taacaccaaa atatcaaaca gcaccatctg tggatgaaat
     7681 catgactgtt cattacgaga aaacagaagg aatcaaagat gaagtcaata agatgcaata
     7741 tgtcgatctt catcaatgga tgccaaaaga tatcttgtta aaggcagata aactttctat
     7801 ggcaagttct ttggaagtcc gagtaccttt attggatatc gaagtcatga aattggctca
     7861 acaaatacca agtaagtttt tattgaatca aaacaacaca aaagatattt tcagacaagc
     7921 tgcgaacaaa catcttccgg aaaaatggtc gaatcgtgtg aaattaggat tccctgtgcc
     7981 aatcaaagct tggttgaaag aagaacacgg ctacgaacaa gtcaaagcat tgttcgaagc
     8041 agattttgca aaagaatttt ttgatcaaga aaaaatcatg aagttgctag atgaccatca
     8101 cgaaggacgt aaagaagagc aaagaaaaat ctggacaatc ttcagtttct tgacatggta
     8161 taaagtctac tttgtagatg aaacgattcc gcaagcagaa gcaatcgact atgtgactgt
     8221 ttgatgaaaa tcaaatatca ataaaaaaga ggttttctcc taccgtttaa cactgagtga
     8281 taagaaacaa tttcaaaaaa cagctcttcc cgttttatag atattatgac ttatggttcg
     8341 gagttggcag aggaacacca ttgtttcgaa cataaggggg ttgtgacacg attttcgtca
     8401 caaccccttt acatatgttc aataatcgat ttctttttct tttgtcccat cttttttata
     8461 aatgaacaaa gttgattctt tgttttgtgc tacttgcttt cctttttcga ttgcttcttt
     8521 ttttgtatca aaatggttgc ttgccttttt tgccccttta gatttcacga tccattgttc
     8581 ttcctcatat tctacgatca catctgaatc aagcagtttt ccagcacgag gattcgtatc
     8641 gtgttcatcc gtcttgcttg gatttttttc tttttcgaaa gttcgttttt ctgattcact
     8701 agcatcttca taccattttt ttgcttgatc aatggcaatt gggatcgcac ggttatcagg
     8761 gtacccttca tctaataatg catttgcgat gtcgattgct ttttttcttg tcaatttatc
     8821 taagtttttc attgatgcag gaaagtcctt catgttccat ggcatattag tatccccctt
     8881 tattaaaaaa cataatctat ataaacaagt gtaagggtat ttatacaaaa agaaaaagat
     8941 tttgtttgag aagtaaaacc gttgtattta tcgtcaagta gttttactta acaaaatcgt
     9001 gaaaaacact agcaatctag aaaaaatcat ggtaagataa tcaagtctga gagtcatctt
     9061 agaggattct tgtaaaaatg ggagggaaaa acatgcgcgt aaacatcact ttagagtgta
     9121 cttcttgtaa agaacgtaac tatctaacaa gcaaaaacaa acgtaacaat cctgatcgtt
     9181 tggaaaaaca aaaatattgc ccacgcgaac gcaaagttac tttgcaccgt gaaacaaaat
     9241 aatgttttta aagttctcaa ttccttgctg gtcaaagaat tgagactttt tttatctcaa
     9301 aacatctaaa atcataagga gcggataata tgtttatttc catgtgggca caagataaaa
     9361 atggtctgat cggcaaaaat ggtttgttac catggcgatt gccaaatgat atgcgtttct
     9421 ttcgcgaaca tacaatggat cgaattttag tgatgggaag aaagacctat gaaggaatgg
     9481 gggatctttc acttccttat cgccatatca tcgtgttgac aacgcagcca aattttcgca
     9541 cgaaagaaaa tgcggaagta atgcattcga tcgatgaact tttgtcgtat agtcaaacga
     9601 tctcagaaga tatctatgtt tcgggtggta gtcggatttt tcaagaactt cttccttaca
     9661 ctggtcggat ctggcgaaca ttgatcgata gcacgtttga aggcgatacg tatattggga
     9721 atattgattt ttcagacttc gcccttatag aggagtatga ggggataacg gatcaagaaa
     9781 atctatatgc ccatcgtttt caaaaatggg aaagagtaaa aaatagggtt gcttcaaaag
     9841 aagaataaaa tctatgcgtc tagtttgtta ataagaaaaa aagcatatca cgactaatcg
     9901 tgaagatgct ttttttagtt aaaatcaatt tagtttttct ttgatatcat tgaccatttc
     9961 ctgactggca ataggtccgt tatatagcca actagattca ggtccgaatt gcagtacatg
    10021 attgtctgtc actgctttaa gattcttcca gcttggttca ttgaacatgg cagcatcttt
    10081 gtcactgttc acaaggatga tgtagtcagc atctaattca gctagctttt cagaggaaac
    10141 agcagaccag tcagaagtgg cttttttgga gacctcttcc actagatcag gaacaccaaa
    10201 ttccagatcg tcgtatacga ttcttccact tgaccgattt ttgctgacca taaaggctga
    10261 gttgttggtc acccacaaca ctgcagctgt cttgttctcg attttatctg ctagttcttc
    10321 tcgagttttc ttgatggatt tttgataatc ggaaatgatc ttttctgcct ctttttcttt
    10381 tgccaaagct ttgccgacat cttttagttg ttcttcccat gtgacatctg ttccgttttt
    10441 tacaacataa gttggcgcga ttttgctata ttgttcgtat ttcccgcctt cgacagtagc
    10501 ggaagaagaa atcaacagca aatcaggctc aaagcttaac actttctcat aaggaaggtc
    10561 ataagaaatc gttgggacat cctttaaatc atcttgcaga taatcttgaa tggaaccact
    10621 gccaacagtc cactgggcga taggtttttc ccctagtgca atcagatagt cttctaagta
    10681 actgccgatg atccgtttgg gcgcgtttgg aatctctact ttatgtccaa gtgtatctgt
    10741 gactgtatga gtactattgg atgaagtcgt cgaattggca gttgtagctg tttctttatc
    10801 tgaagagggg gagcaagcgc tcagtactcc agccagcagc aatatgctag ataatgtgaa
    10861 tacttttgtt tttaatttca tttctttcct cctaacataa ttgagaatct ttctcattgg
    10921 ttattattcc tgatatttcg aacgctgtca accgcttcat ttggctatag atatgaaagt
    10981 agcgcttgac gaaatgttga aacagcgtca taattgagaa tgattttcag cgaaaggatg
    11041 ggtgaaatga cgagattaag agcagctgac attgcagccg gctacgaaaa caaaaaaatc
    11101 attgaaaaca tgtctgttac gattcctgaa gggaaaatca ctagtttgat tgggccaaat
    11161 ggaagtggaa aatctacttt attgaaaagt tttacccgta tactacctgt gaaatcagga
    11221 aagatcattg tcaataactc tgatatctct acatacagtt caaaagaact tgcaaaaaaa
    11281 attgcattat tagcacaaac tagtgaacac cctacaggta tgacagtaga agaagtagta
    11341 tcttacggac gataccctta tcaaaaactg ttcagtggca tggacacgca agatcatgaa
    11401 gcaatcgagt gggcgttgga tgctacaggt ctagctggat ttcgtatgcg ggatatcgca
    11461 acgctgtctg gtggacaaac ccagcgtgtc tggatcgcta tggcgcttgc tcaggaaaca
    11521 gatatcttga tcttggatga accgacaaca tttttagatc cggcacacca attagagatc
    11581 ctacagttat tgactgaaat caatcaaatg aaacaaacaa cgatcttgat gtcgattcat
    11641 gacattaatc atgcttcacg tttttcagat tatttattag caatgaaaaa cggtaagttg
    11701 atcacagaag gtacacctga agaagtgatc acaaaagagt gtatgaaaaa aatatttgag
    11761 attgatgcaa cgattgtcac gcttccaaga acaaataaac cagtttttct aacctatgat
    11821 ttgttgccaa aagagcagga ggatcaatta tgaggactat gaagaaaaat agactcctgc
    11881 ttttggtctg ttttgttctt ttgattattt tttttgttgc tctttcaaat ggcgcagcga
    11941 ctgtgggaaa agcagatttt tggcaggcgt tgtttgattt tgatagatca aaacaaagtc
    12001 accaaatcat acgaaatatt cgtttgcctc gtgtggcggg agcatttctt gtagggagtt
    12061 gttttgcatt gagcggtgca ttgatgcaag gagtgacaag aaatccttta gcagattcag
    12121 gcttactagg aatcaacgcg ggtgcttcgt tggcaatggc ccttagtttt gctttttggc
    12181 ctaaagcttc tgatttttct gtgtttctaa tgtcattggc aggttctttt gctgtaactt
    12241 tgctcgtatt tggttttttg cagttttcga aaatgggcat gaatccttta cagctgattt
    12301 tagctggcgt agctattagt tcttttttta ctgctatcag ccaagcactt acccaattct
    12361 ttaatctgca gcaggatctt gctttttggt ttgtaggcgg agctgccaat atcacatgga
    12421 aacaattagc agtattgttt cctttatata tacttgctgt tttggtttct ttttcattag
    12481 gaaaagcaat atctttgatt catttgagtg aaacacatgc attagctctt ggaagtcatc
    12541 ctaaacgaac gcggttgatt gttttctgtc tagttgcagt gttagcagcc attgccgttt
    12601 cgttagtcgg acctgtttca tttattgggc tgttggttcc gccagttgtt cgcggaatgg
    12661 ttggaacgga ctatcgttat gttctacctg tctctttttt agttggcggt ggattggtta
    12721 tgttggcgga ttttacagcg aggatgatca atcccccttt tgaaacacct tttggattag
    12781 tcattgcatt gattggcgtt ccgtttttac tgatacaagt aaggagggaa caacggtgaa
    12841 aaaaatatat tggacgttct atttcttgct ttttgtactt gtcatcctgt ctataggtag
    12901 tttaatgatt ggtacgcctt ttatttacct ggatcagctt tttgccacgt tcatagggaa
    12961 agaatcatct tctttggcgc tgattgttag agaatttcga gttcctcgat tactgatcag
    13021 tttattagca ggagcttgtc ttggcgtttc tggttatatc ttgcaaggag taacaagaaa
    13081 tgaattggca gattctagta tattgggcat taatgccgga gcaggatttt tagtgatgct
    13141 ttatttaggt ttcttttcgc actattcgct gccgtttcta ttgctagttg tagcctttct
    13201 aggtggtgtt ttagctgctt gctgtgtcta tcttgcggcc tatgatcgaa atggcttttt
    13261 gggtatgaat cgaatcttat tgtccggtat tgccgttaat gccggccttt ctgcattaac
    13321 attgctatgc acgatccaat tatctaagga aaattatgga tttgtgaatg catggttagc
    13381 gggatcaatc tggggtgcga gctggcctta tgtttgggca ttgcttccat gggcggttat
    13441 cttaattccg ctaggaggac tttatgcaca gaggattgta ttgctatcat ttggtcaaga
    13501 acgcgcgcaa ggattaggcc ttaacgtggc taaaagtcaa aaaatccttt tgttgttagc
    13561 tgttgctttg gcaagcggta gtgtagcggt gacaggtagt ttatcttttg taggactact
    13621 agctcctcat gctgcgaagc tagtcgtacg taaagaaaat aactggacct ttgttctgag
    13681 cggtttgttc ggttccattt ttctgcttag tgcagatatg gttgctagag tgattctact
    13741 aagtggggaa gtacctacag gaattttgat tgcccttttt ggtgcgccat attttctgta
    13801 tgcacttttt atcagacaaa aacacgcgct gaacagttga aattcattaa aaaaggaagg
    13861 ccaatcattt ctaggtcttc tttttattct atttatgcat ttatttgaaa gttcgattgc
    13921 tatttttctt tttttcctat accattttat gagagaagga agggtgagtt tttggaccaa
    13981 ttgaaagtaa gattacgccg gaaaggaatc gaaaagctga aaaatctggc caaaagtggc
    14041 aagaaaaagg aaaaagaaca gcgaatctat aaacagctat tcgccagcga gtactggaag
    14101 tcagcaaaag tgatagggac gactttatcg aatgaatttg aactggatac acagccggtt
    14161 atccaacgag cagaaaaaga acaaaagaaa atagttgtac ctaaaacttt gccgaaacgt
    14221 cagatggcgt tttatgaaat ggatgaacag tccgttcttg tacgttcgag ttttggtgtt
    14281 cttgagccac aatcagagaa gctctatttg ccggagatga tcgatctcct cattgtacca
    14341 ggtattatct acaatagagc cggttatcga atcggttttg gcggtggtta ttacgaccgg
    14401 tatttaaata attttcataa aaaaacatgt agccttgttt ttcgtgaaca attgaatgaa
    14461 caatggcaac cggagagctt tgatcagaag atccaacaat tgtttattga ctaaaatgaa
    14521 atgggaggtt ttcatgaatt atcaacagca aataaaattg cgtgcatggt taagacgacc
    14581 gttatggacc tatgcttttt tagggataca gacagcggtc ttcctcatga tggaattatt
    14641 tccgcgactt gagatccctt attatgccgg aatgtatggt ccttatcttg ttcacttcaa
    14701 tgaatggtgg cgtttagtga caccaatctt catccatttt ggactgatgc atttcgtaat
    14761 gaactcactg atactgtatt ttatggggca gcaaatcgag gcaatctatg gccattggcg
    14821 ttttttcttg atttatatgt tcagcgggat aatgggaaat acagcaagct ttgcttttaa
    14881 cgaagcgaat gttctgtctg gtggtgcaag tacttcgata ttcggtttgt tcggtgcgtt
    14941 gtttatctta gggtttcatt ttaaacataa cccagcgatc cagcagctag tcagacattt
    15001 tcttttattt attgtgttga catttgtttt tggtcttctg gatacctctg tagatgtttg
    15061 gggacatgtc ggtgggctaa ttggcggatt agtgttaggg aatgttttgg gtctaccgaa
    15121 acaacagaca agctattcga tacaccaaag gattctttct acactagttt ttgtatttct
    15181 ttttgtaata tgtattttac tgggtttaaa aaaatatggt ttacttgtat aatatgaagg
    15241 aagtgcttaa agatggagag cttatatgat gtacagcagc tgttcaaaaa atttggcatc
    15301 tttatctatg tgggtgcacg catctatgac attgaattaa tgactatcga attgcgtaat
    15361 ctgtatgata gccgtttgat tgatcatgat acatacatga cagcgtggcg catattaaaa
    15421 agagagcacc gaattgaaga aagccgaaag aaaggaaatt tgtaatggac aagaagatta
    15481 ttgggattga tttaggtgga acaacagcaa aatttgcaat tttaacacca gaaggagaga
    15541 ttcagcaaaa atggagcatc gataccaata ttttggatga tggtaagcat atcgttcctg
    15601 aaatcattga atcaatcaat catcgtttga atttatataa tatgaaagcc gaagacttta
    15661 tcggaatcgg catgggtacc ccaggaagcg tggacagcga agctggaacg gttattggtg
    15721 cgtataactt aaactggaca gaagttcaat ttgtgaaaaa actgatcgag gcaggaacag
    15781 gaatcaaatt cgctatcgat aatgacgcga atgttgctgc tttaggtgaa cgatggaaag
    15841 gtgctggaga aaacgatcca gacgttgtat tcgttactct aggaacaggt gttggcggag
    15901 gtatcgttgc tggcggaaat ttgattcacg gcgtgagcgg agctggagga gaaatcggac
    15961 atatcactgt ggatcctgaa gggttcgaat gtacttgcgg taaacgcgga tgtttagaaa
    16021 ctgtatctag cgctacaggt gtggtacgcc taggtcgttt cttggcagaa gaatacgcag
    16081 gggattcaaa actgaaagca atgctagaca acggagaaga agtaacaagt aaagatatct
    16141 ttgaaatggc tcaagaagac gatccgtttg cattgatggt tgttgatcgc gtatgcttct
    16201 acttaggttt agcttgcgga aacttgggga atacattaaa tccgtcaagt atcgtgttag
    16261 gtggaggagt atcggcagca ggagaatttt tacgtagccg tgtagagaaa tattttaaag
    16321 aatttacttt cccgcaagta agagaatcaa caaaaatcaa attagcagag cttggtaatg
    16381 aagctggtgt cattggtgct gcctcattag ccttgaaatt tgcttaaaaa aacggaggca
    16441 ttacatggtt ttatgggtaa ttaatatcat tttgctattg attgtgcttg caatcatcct
    16501 ttgggaagtc tatctacgag taatggccaa acgttcagca acgactttga cagaagaaga
    16561 atttcgagaa ggaatgagaa aagcacaagt aatcgatgtt cgcgaaaaag atgtgtttga
    16621 tgctgggcat attttaggtg caagaaacat cccttacact gttttgaaag attcattagg
    16681 atctattcga aaagaccagc ctgtctatat ttacgatcaa aagaaaagtt tgagcatccg
    16741 tacagcaaat aaattacgta aagcaggtta tcaagagatt tatttactta agggtggata
    16801 tgacggatgg tctggaaaaa tcaaaagtaa aaaagtataa aacagatgat tgtatgacag
    16861 ctttaaaaac cgaatcaatc attagatttt gaccaagatc acatgaacgg cgcaatggat
    16921 agttgtgccg ttcatttttt ttattttgtg ctattatcta catatataag ctaagcgaaa
    16981 aggtagaaag aaggaaacaa ctatgatcaa attaaaacgt gtttaccttg atgctgaaaa
    17041 agaggatggc tatagaatac tcgtggatag attctggcca cgaggaattt caaaagaaaa
    17101 agcgcagtta gatctgtggt tgaaagaaat tgcacctagt aatgaagcac gcaaaacttt
    17161 tgaccatgta ccagaacgat ttgcttcatt tgcaaagaaa taccagcaag aattgatttt
    17221 cgaagaagaa aagaagcaag ctatttttaa actcagtcaa cttatgaaag aacatccagt
    17281 cattactttt gtttatggtg caaaaaatga acatcagaac catgctgttt tattgaaaaa
    17341 atttatcgaa atggaggcat gcaaatgaaa atagggatca ttggtgccgg acctagagga
    17401 ttaagtgtag cagaaaggat tctccataat aatcaggaca gacattctct tgaacttttt
    17461 cttttcgatc ccaatggtcc tggaggtcga gtttggcggt tgaatcagcc accggagtta
    17521 ttaatgaatt ctgtttctca acaagtgacg ctttttacag atgaaacatt gtccagtgga
    17581 ggattggtaa cacagggacc caacttgttt caatggagta aaggacctgc tacagattat
    17641 atcgaacaga cggtaaccaa aaataagcaa ctaattatag aagaaatcaa tcgtctggaa
    17701 aaagatcagc aatctactcg atgtttatac ggactctatc aacgctggtt tttctcaaag
    17761 ttgcaagaag aatttccagc ttctattcag tttgtttatt ctttagtaaa aaaagtaagc
    17821 aaaagaaaag atggttatct tttacagaca gatcagcaaa gctattctat cgatcagctt
    17881 gttttagcaa gcggtcactg ggaaaatgat gagacagaag aagaaagagt gtgggtagac
    17941 tacgcaaaga aaaatgtttt actgtatcag cttccttcta atcctgcaga tgtagatctt
    18001 tcgaagatac cgcctaaaga aaatgtcttt ttaagaggtc taggcttaag tttttttgat
    18061 tatgtcggat tgtttacagt atcaagagga gggcgcttcg agaacgaaaa gggaaaactg
    18121 gtctatcatc catcaggcaa ggaaccgatt gtatacagtg gatcaagaag ggggctccct
    18181 tattaccctc gaggaagaaa tcaaaagcaa ggcggagcaa tggcatggcc acggttacta
    18241 acaaatgaaa acctggagca atggcatcag tcgggatttt tgactggcga aacatttttt
    18301 gactatctga aaaaagacgt tgagttgttt tattacaaga aaatcatcga agaatatcac
    18361 ttgccgattc caaagaaaaa ttttgagcat gattttctct ccctctcttc agaggagtgt
    18421 ctcggaaagc atccagcact aatcccttat agatgggatt ggtcgtattt agaaactcct
    18481 cttgcctatc agaatgagcc cttttcagaa gctagccgaa tatttattga agggcagata
    18541 aaagaagcgg aaaaaggcaa ttgcacagga gcatttgctt caacatttga tgcattaaaa
    18601 gactggcgtg atccaatccg gcaggctatt gaatggcacg ttttttctgc taaggaatac
    18661 aaagaaatac tatggggatg gtttactcat ctaaatagct ttttaacgat cggtccgcca
    18721 atcattcgga cgaaagaatt agcagcactg atagatgcgg gtatttttca tttgatcgaa
    18781 ccgccatttg atattcagca aaagcacaac cgctatgtta tttctctaca agataaagaa
    18841 atcagcagtc gctatttgat tgaagctcga ttgccacaaa caagcttttc tcgttcaagt
    18901 aatcctgtca taaaaaattt gaaggaaaac aaacttgttc gccctttttc attttcagat
    18961 aatacaggaa gttatcattc agaagctata gatgttgagc gaggtacgaa tcgcgtgatt
    19021 gatgcttcag gggaaatcca gtctgacttt tattgctatg gaatacctgt tgaaggtgtg
    19081 gattggctaa ctgcagcagt agctagacct tatacagatc catggaatct gagacaggca
    19141 gataaaatag cacaacttat attgactgac aaataaaaat acgcatgatg acttatctgt
    19201 tttttagaaa gtcttcatgc gtatttttaa ataattatta acgagaaacg cgtttacgca
    19261 ttgctttttt acggttttct tcgatcattg cttctttttc gtcaattgga tcgatcactg
    19321 tttttttcac agaagcaacc aaaccagcaa cggcagcggc tgttgccaga cttcctacta
    19381 atattccaga tacaaatttt ttcattgaat ccacctcatt tattatttgt tatatctatt
    19441 atggaacaaa agagaaaaaa aggctagttt tacgtttgtt tgtaaacctt tgcaaaaaat
    19501 ccttgaaaat gtttactaaa tgttttatta taaatttacc atatgaattt acgaaatttt
    19561 actttgtttg ttttcaccat aaaagtaaac ttcgtttttt aaattatata gaatcaacaa
    19621 acacaatttt actaatcaaa ggagtgtttt ttatgacaat tttagtacta ggcggagccg
    19681 gttatatcgg ttcgcacgct gtagaccagc tagttcaaaa ggggtatcaa gtagctgtgg
    19741 tagataattt gctcacaggt cacaaacaag cagtacatcc agatgctcat ttttatgaag
    19801 gagacatccg ggataaagaa tttctacgtt ccgtatttga aaaggaaccg attgaaggag
    19861 taatccattt tgctgcaagc tctttagtag gagaatctgt cgaaaaacca ttgatgtatt
    19921 tcaacaacaa cgtatacggt atgcaaatct tacttgaagt catgcacgag ttcaacgtga
    19981 ataaaatcgt attctcttct actgcagcaa cttatggaga gccgaaagag tctccaatca
    20041 cagaagatac accagctaat cctaaaaatc cgtatggtga aagtaaattg atgatggaaa
    20101 aaatgatgaa gtggtgtgac caagcatatg gcatgcgcta tgtcgctttg cgctacttca
    20161 atgtagccgg agcaaaagca gatgcatcta tcggagaaga tcatacacca gaaacgcatc
    20221 ttgtcccaat cattttacaa gtagctcttg gtcaaagaaa agccttggct gtttatggag
    20281 acgattatga tacaccagat ggaacatgta tcagagatta tgttcaagta gaagatttga
    20341 ttgctgcaca tattttagct ttggaatact tgaaagaagg aaatgaaagc aacttcttca
    20401 atttaggcag cagcaaagga tactcagtca aggaaatgct agaagcagct agagaagtga
    20461 caggaaaaga aattccagca gaaatagcac caagaagagc tggagatcct agccgattag
    20521 tcgcttcaag tgaaaaagcg agagagattc tgggctggaa acctgaatac acagatatca
    20581 aagcgattat caaaacagca tgggattggc atgtcagcca tccaaatggt tatgaagaat
    20641 aggggaatga gatatgtcaa tcagtcaaac gatttgtgat tttgccacac ttgcgatcga
    20701 agctggcgga tggatggaaa tggaccgctt atatttacag aatcgtcttt tagggatggt
    20761 aggagaagat gctctagaaa gcattgaagt aagaccagtt aaggaagaat ctgtagactt
    20821 attggatcag cttgtcgcta aggcaagaga aaatggtgtt ctttcagaat tgccagcaga
    20881 acaagagatt tttgaagcgc aattaatgga ttttctcaca ccgccacctt cagtcgtcaa
    20941 tgcattcttt gcacagcact attcgaaaag cccagaggaa gcaactgagt atttctttaa
    21001 tttaagtaag aaaaatgact acatcaaaac aagagccatc gcaaaaaaca tcaaatttcc
    21061 ggcagagtcg gagtatggtg aactggaaat cacaatcaat ttatctaagc cagaaaaaga
    21121 tccaaaacaa atcgcagcag aacgcgaggc ggtaaaagta gactatccta cctgcatgct
    21181 ttgcatggaa aacgaaggat atagaggtcg cttgaactat ccagcccgca ccaatcaccg
    21241 gattattcga atgaatctag acggtgaaag ctggggattc caatattccc cttatgctta
    21301 ttacaatgaa cattcgatca ttttatcaga acaacaccgc ccgatgaaaa tcacaaagga
    21361 aacatttgct agactgcttc ggattactga agtcttgcct cattactttg ttggttcgaa
    21421 tgctgactta ccaattgttg gaggatcgat tctttctcat gatcattatc aggcaggacg
    21481 tcatgaattt cctatggcaa aagcaccgat agaaaaactt atcaaactag aagattatcc
    21541 gcaagtcatc gcaggagtcg tgaaatggcc gatgtctgtg atccgtttgc aatcaacaga
    21601 gaaaaacttg cttattgaag cagctaactc agtattggaa aaatggaaag catattcgga
    21661 tgaaacagta tctatcagag ctttttcagc agatggtaca ccacaccata cgattacgcc
    21721 aattgcacga agaagggaca attactttga gttggacctt gtccttcgag acaacaatgt
    21781 ttcagaagaa catcctgatg ggatttttca tccacataaa gatgtccaac atatcaaaaa
    21841 ggagaacatt gggttgatcg aagtaatggg gcttgctgtt ttaccacctc gtttgaaacc
    21901 agaactagta gaagtagagc atttcttatt aggagaagaa aaccagatgg ctgaatatca
    21961 tcgtccgtgg gcagaacaat taaaaaaaga acatcctgat ttgacgaaag atacggtcac
    22021 cgatgtcgta caaaaatctg taggtcaggt ttttgcacgt gtactggaag acgctggagt
    22081 attcaagcga gatgaaaaag gacaggcagc gtttgaacga tttactgcga ttttataaac
    22141 gtaaaaaagg aggagagaca aatggcaaca attaaagaca ttgcgcagtt ggcaggagtt
    22201 tctcctgcta ctgtttcaag agttctcaac tatgatcctg atctttccgt tggcttagcc
    22261 acaaagcaaa aaatttttga agcagcagaa cgcttaaatt atacgaaaca tcgtaaaaat
    22321 gcagcaaata ctaagcaaac gcttcgcctg atccagtggt acgacgatac ggaagagctt
    22381 gaagatcttt attatttatc tattcgccta ggaatcgaaa aaaaggcaga agaagcaaac
    22441 gttactttat tgaaagaaac gtgggattcc attagtgatc ttccggcaga tggaacgatc
    22501 gcactaggaa aattcgacga agctcagtta ttagaaatga aaggatccca aaatgccttg
    22561 cttttggtag attctgacgg cacttctcaa ggaatcgact ctctagttgt cgactttaag
    22621 tcaagtgttc aaaaagtcat tgaacacttt gtctcccata aagcgcaaca tatcgctatg
    22681 cttgctggaa cagaatatac gaagaaaaac catcagcggc taaaagatcc cagaattatg
    22741 gcatttcaac aagttttggc agaaaaaggc tacgacgaag gtccaattat cgaagcagat
    22801 ttttctgtcg agtcaggata tcaagcagtg aagcagtatt taaaaacgaa tccgcatgtt
    22861 cctgatgcgc tttttgcagc taatgatgca ttagcgattg gcgcactgaa agcgattcaa
    22921 gaaaaaggtt tagtggtacc agaagatatt tctgtgatgg gcttcaatga tgtcagtgta
    22981 gcgaaatatg taacgccctc gctttctact gtaaaagttt ataccgaatg gatgggagag
    23041 ctagcagtgg agacgattct tcaattaagt aattccaaag caccggtttc aagaaagatt
    23101 attgtggaaa cagagttgat tattcgagaa tcaacgaaat aaaagatgga aaagctgtgg
    23161 gaaaatggag tgataccgca gcttttttgt gtttcaggaa ggggcaaaat ttgcccgcca
    23221 gcagataatc atgtattatt gtactagttt tgcctttggt attttcatcc tcagaataaa
    23281 aaagtatgct acaatggaag gcgagaatgt catagcagaa aggatcaaat atatgattac
    23341 attaggaata gatactgcaa accaaacttt ggcgatcggt gtggtagaag acgaacaaat
    23401 tcttggacaa atacaaacaa atatcaaacg caaccacagt gtaacactga tgcctgcaat
    23461 cgaccaattg tttgcagatc ttcaaataag tccaaaagac attgaccgaa tcgcggtttc
    23521 tgatggtccc ggttcttata cagggcttcg tataggtgta acgacagcaa aaacgattgc
    23581 ttatacatta gataaagagc tagtaggagt atctagtctg aaaaccatcg cggctaattg
    23641 catcggtgtg gataatatta ttgtgcccat gtttgatgct cgaagaaaaa atgtatacgc
    23701 tggggcatac cgctggaaag aaaatagatt agaaactgta cttgaagatg tacatatttc
    23761 tgcttctgat ttgttcacac aactacaaaa gctgaatgaa gctatttatt tcgtcgggag
    23821 tgattgtcac aaattcttct ctgaaataaa ggaaatattg cctagtgcga tgattaatac
    23881 agttccagca tgggatattc caagtggtgt aactgtggct caattaggtg ctttagttga
    23941 accagttgca gagaaacaag cttttcttcc tcgctattta aaacgagtag aagcagaaga
    24001 aaaatggttg gagacgcaca cacctggaga cgaaagttat gttgaaaaaa tttagagcgt
    24061 atgccagaag cttttttcaa gataaaatga tttatacaga aaaaacagtc gaacaaatcg
    24121 atcgtcgtta cgtactaaga gaattgacta cagaagatat caaagagctg ctggatgtcg
    24181 aacgagaagt ctataatggc gaattgccat ggacaaaaag tgcctttata tcagaattga
    24241 agtcaccgct tcctcatcta tatctaggta tctttgataa tggaagaatg attggtttta
    24301 tcggttcaag agcgttcggt ttagattgcc atatcacgaa tattgccgtt cgaccggcat
    24361 atcagcgtaa acgaatcggt tcgctgctta ttgacgaaat cgagaacttt gctataatga
    24421 atcgctgtga gacgatgtca ttagaagtgc gtatgagtaa tcaagatgca caaagacttt
    24481 atcggaaatt ggggtttcaa gcaacaacga tcaaaaaaag atattacact gaaacaaaag
    24541 aagatgcgtt ggatatgata aaacttttag aaaatgagtg agattagttg atttgcagaa
    24601 aaaaagattt tgatgaagcg tacttggcag aatgcgtatg gatgattagc gaaaatgctt
    24661 atccacatgg ttcaccttgg acgaaaatgc agtttttggc agacatccaa cagcctcact
    24721 ctgattatct gcttttagct gaagataacc agatctgtgg ctttattgga tactcgaaag
    24781 taatagatga agtcgagatc acaaatattg ctgttgcagt ttcagaacaa ggaaaagggc
    24841 atgcacggca tttgttacaa ttactgatta cggaacaaat aaaagattcg ctgcatgttt
    24901 ttttagaagt acgtctatct aatgaagcag caagaaaact ttatgaatca gaaaagtttc
    24961 gtatattagg taaacggaaa tcatattatc gcaatccgac ggaagatgcg atcattatga
    25021 gcagaaagtt aaaaacggag acgatgaaat gaatgaagaa ttgattctgg cgattgaatc
    25081 aagttgtgat gaaacgagtg tagcggtcgt tagaaatgga acagagatct tatctaacat
    25141 cgttgcttct caaataaaca gtcataaacg ttttggtggc gtagtacctg aagtagctag
    25201 ccgccatcac gtagaacaaa tcaccttatg cttggaagat gcactggtag aagctggtgt
    25261 gtcggcagaa gatttatcgg cagtcgctgt cacttatgga ccaggacttg tcggctcgtt
    25321 gctgattggt atttctgcag ctaaggcttt tgcttgggca caccaattgc cgcttattcc
    25381 tgtaaaccat atggcagggc atatctatgc agcccgtctg gttaaaccat tccagtttcc
    25441 attgatggca cttcttgtta gcggaggaca tacagaatta gtctatatgc aagaagatgg
    25501 atcatatgaa atcattgggg agacaagaga tgacgcagca ggagaagctt atgacaaagt
    25561 tggaagagta ttaggtcttt cttacccaag cggaaaagaa atcgatcagc tagctcatca
    25621 gggaaaagat aattaccatt ttccgcgagc gatgatccat gaagataatt atgatttcag
    25681 ttttagcgga ttaaaaagtg catttatcaa tttggtgcat aatgcacaac agcgtggaga
    25741 agatctagac aaaaatgatt tagcagctag ctttcaagca agtgtgatcg atgtattgat
    25801 tactaaaaca cttcgagctt gtcaaaatta tccagtcaag caactgatta tagctggagg
    25861 tgtagcggcc aatcaagggt tgcgtgaagg attgcaggat gctttggcaa ctaaactccc
    25921 tgaggtagaa ctagtgattc cccctcttcg actttgtgga gataatgctg caatgattgg
    25981 agcagcagca catgtggaaa tgcaaaaaag aaattttgct agctatcagt tgaatgctga
    26041 tccaagcttg gttttatctg aataaagagg ttgtgaaaaa gctgccctgc atcaagtaat
    26101 aagaacagcc caggaaaaac ggcttctcat gtttttgacc tggctgttct tattaccgcg
    26161 tatgcaagct tttgaacgcc gtttaatcaa gagttcgtaa gcctgtcgtt taactctgat
    26221 tcacaaggaa caatttcaaa aaacagctcc tcatgtttat gaacattatg acttgtgacc
    26281 gaagagttga caggtacaca ccgtttaatc gaaatagggc tgtgacacgt ttttgtcaaa
    26341 gccctatttt ttacatactt ttaatcatta ttgttatttt gctatttttt acagcttatt
    26401 tatcattatc cgttcctctg attgccctca ctgagtaatc gtttattgtc gaggaatatc
    26461 gaataatcta gatcggatga ggacaagacg cttcatctag ttaatctaac agtatttttt
    26521 gcaaacagtg aatattacat aactgaaaca cacatagaaa atctttgagt cttgtccagt
    26581 gatctaaaac tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat
    26641 atttatcagg gtcactgctt atttttgttc gcgttattct ctcatagctg gagtagtagc
    26701 tgtcttacag aaagtaaaac gctttttgtc ataatctaac agcccggaat cacgtagttg
    26761 tttgattact tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta attcttttaa
    26821 ggtgatcgga taaggaatgg tgatatgacc atcttttgtt ggtacgccca attcttcttt
    26881 caaaatgaat aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc cgatttggat
    26941 acgcaattca tgtatttcaa ctactttaga aattttttgt atcatataga tcaactgttc
    27001 gggatttttt cgtgctaatt tttcatagta ggaaacagga aggtagaatg tttcaatatc
    27061 agtcatcgct tgagctgtat aagtgtaggt ctcttctcca aacattcctg acataggaaa
    27121 aaggttattt gtattcacat agtcatagta gtaataagtt gcactttcat cataacgttc
    27181 taaacgaacg agtcctttgg tcaagatata caaccgttgt ctgggatcac catcgtcgaa
    27241 aaggatctgt ccttttttat aagaacgaag aacagtatgt tccttgatct gttggaactc
    27301 ttcatccgag aaatgttcaa aatcaggttg atatctgagt tttgaaaagt gaaaatcgat
    27361 tttattaaca cgcattaaaa agtcctctcc ttattgttca ctatatttat tgtcactttt
    27421 tttaaagaat ttgtaaagtt ttaacatacc aacattagaa acaagtttta atactttaca
    27481 aatataattt gttattatgt aaaaaatata caatggtatt gaataatttg ttttgaagcg
    27541 aatgatttgt gtataaaaaa
//
LOCUS       DACEJZ010000033        26705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEJZ010000033 DACEJZ010000000
VERSION     DACEJZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.033"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..2181)
                     /locus_tag="IW656_001288"
     CDS             complement(142..2181)
                     /locus_tag="IW656_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0837003.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2239..2994)
                     /locus_tag="IW656_001289"
     CDS             complement(2239..2994)
                     /locus_tag="IW656_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0837004.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(3086..4216)
                     /locus_tag="IW656_001290"
     CDS             complement(3086..4216)
                     /locus_tag="IW656_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0837005.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(4757..4963)
                     /locus_tag="IW656_001291"
     CDS             complement(4757..4963)
                     /locus_tag="IW656_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837006.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4979..5593)
                     /locus_tag="IW656_001292"
     CDS             complement(4979..5593)
                     /locus_tag="IW656_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0837007.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(5740..6423)
                     /locus_tag="IW656_001293"
     CDS             complement(5740..6423)
                     /locus_tag="IW656_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0837008.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(6532..6990)
                     /locus_tag="IW656_001294"
     CDS             complement(6532..6990)
                     /locus_tag="IW656_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0837009.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7074..8837)
                     /locus_tag="IW656_001295"
     CDS             complement(7074..8837)
                     /locus_tag="IW656_001295"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0837010.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9058..10518)
                     /locus_tag="IW656_001296"
     CDS             complement(9058..10518)
                     /locus_tag="IW656_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837011.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(10726..11652)
                     /gene="glsA"
                     /locus_tag="IW656_001297"
     CDS             complement(10726..11652)
                     /gene="glsA"
                     /locus_tag="IW656_001297"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0837012.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(11675..12886)
                     /locus_tag="IW656_001298"
                     /pseudo
     CDS             complement(11675..12886)
                     /locus_tag="IW656_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(12921..14534)
                     /locus_tag="IW656_001299"
     CDS             complement(12921..14534)
                     /locus_tag="IW656_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0837013.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFYLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(14646..14855)
                     /locus_tag="IW656_001300"
     CDS             complement(14646..14855)
                     /locus_tag="IW656_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ0837014.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            14946..15134
                     /locus_tag="IW656_001301"
     CDS             14946..15134
                     /locus_tag="IW656_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837015.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            complement(15844..17058)
                     /locus_tag="IW656_001302"
     CDS             complement(15844..17058)
                     /locus_tag="IW656_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0837016.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(17186..17656)
                     /locus_tag="IW656_001303"
     CDS             complement(17186..17656)
                     /locus_tag="IW656_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0837017.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(17653..17895)
                     /locus_tag="IW656_001304"
     CDS             complement(17653..17895)
                     /locus_tag="IW656_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ0837018.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(18441..18971)
                     /locus_tag="IW656_001305"
     CDS             complement(18441..18971)
                     /locus_tag="IW656_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0837019.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(19153..19809)
                     /locus_tag="IW656_001306"
     CDS             complement(19153..19809)
                     /locus_tag="IW656_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0837020.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(19936..20409)
                     /gene="dfrF"
                     /locus_tag="IW656_001307"
     CDS             complement(19936..20409)
                     /gene="dfrF"
                     /locus_tag="IW656_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0837021.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(20828..21046)
                     /locus_tag="IW656_001308"
                     /pseudo
     CDS             complement(20828..21046)
                     /locus_tag="IW656_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21077..22291)
                     /locus_tag="IW656_001309"
     CDS             complement(21077..22291)
                     /locus_tag="IW656_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0837022.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(22632..22874)
                     /locus_tag="IW656_001310"
     CDS             complement(22632..22874)
                     /locus_tag="IW656_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0837023.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(22906..23463)
                     /locus_tag="IW656_001311"
     CDS             complement(22906..23463)
                     /locus_tag="IW656_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0837024.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(23476..23664)
                     /locus_tag="IW656_001312"
     CDS             complement(23476..23664)
                     /locus_tag="IW656_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0837025.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(23677..24243)
                     /gene="amaP"
                     /locus_tag="IW656_001313"
     CDS             complement(23677..24243)
                     /gene="amaP"
                     /locus_tag="IW656_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0837026.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            25473..25946
                     /locus_tag="IW656_001314"
     CDS             25473..25946
                     /locus_tag="IW656_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0837027.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            25955..26182
                     /locus_tag="IW656_001315"
     CDS             25955..26182
                     /locus_tag="IW656_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837028.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         7792 a         5476 c         4194 g         9243 t
ORIGIN      
        1 aaatatttta caaaaaaagt ttatgaagtg tgagtaaatc aaaaagaagt tgtgaaagag
       61 acatcgtgaa aaccgtttat ttgaaaaaag gactgtgacg ataacgccac agtcctttta
      121 ctgtacttaa aaagcaattt tttattgata attttggttg aggtattgca atagttccga
      181 tgctcgtttg gttgctgaat catcatcttc tttattttcc aacatgctga ccatcatata
      241 accttggtta tcaagattga aagcaaacaa gaaactgttc tcttgcccct ttacatcctg
      301 tttttctttg atttccgctg taccagtttt cgctgccaac ggaatcccta aagcagaaag
      361 agaatgtgct gtaccattta catcttgcac aacttctctc agatctggca cgatcgtttg
      421 tactgctgtt tcgctgatga cattcttctt atcttttgtc tctttatctg caatcaattt
      481 aggatagaca agtgtgccat tgttggtaaa aacagaatac attgctgctt gctggatagg
      541 agtaattaga agttcgccct gtccatatcc agtatcagct agcaagatat ctgagttaaa
      601 gctatcttca ttagaaattt gtgctggatt catactaatt ggcaaatcaa ggtcttcacc
      661 aaaaatgaat ttatctaagc ctgtacgaaa ttttttctca cccattttca acgtttcttg
      721 tgccgcatat atattatcgg aatagatcaa agcagttttt aagtctactt gtgatacatc
      781 actactgaca cgcgttactt gataagatcc ccaagaactg tctttttgcc atttaagccc
      841 gttgatcgtc aacacttcat ttggatcgat agtgccgttg tcgagaccga ttgctgctgt
      901 aatcatttta aacgtagatc caggagcata acctgtcgca aatcggctga tgaatggatg
      961 ttcaggattt tcttcataag ctttgtaatc ttcttgtgag atcccgtttg tcattttgtt
     1021 tggatcatag cttggagagc tagcaagcgc aagaagatca ccggtttttg gcgttgtcgc
     1081 aacagttgat ccagcttttc ctcctagact gtcaaaagct gttttttgtg ccttcgcatc
     1141 gattgtcaat ttgatatctt tgccattttg aacttcatgt tcgatcagaa cttttttctc
     1201 tacaccgtct gcatctgtaa tgcttaattt cccacctgtt gtcccacgaa gatccttatc
     1261 aaaagccatc tccaaacctg agcgaccgat ttttccatta ctgcttaatt ctggattctt
     1321 atcaatatct tctgctgtga tatctcccac gtaaccaatc agctgagcag ctgcttcacc
     1381 caaaggataa tatctgccgt ctacttcttg aatggtagct ccagctggaa gttctggcgt
     1441 tgctccatca atgattttca atgggacaaa gtaatcgggt tgtacccagc tttgtgaaat
     1501 cgcctgattg atagcatctt ctgttaagtc gaaggaggaa gcaatcgctt tgatattggc
     1561 tgttttttcg cctccatctc caagtttact tggaacgact cctaattgtt ttagtttgcc
     1621 tgttgttgct aatggttcac catttcgatc tataatattc cctcttgctg cttcttgtgt
     1681 ggtcaggctt actttgtcat ttccttccat ttctggaaaa accaagttgg gttgccagtt
     1741 gatcgtggtt tgaccatcat ttctgtctaa tgttccttta taagaaagat ctttcaattc
     1801 acctaatgag gtattcatct ttgctttata tgaaaaactg taagtagaat cgtctttttt
     1861 atctactttt agattcgata tctgaagtcc tttgacatca gcagcaccgt atatattctg
     1921 gtatttatct aagatctctt tttcagataa tgcactttta tttgccgcct tttttgatgt
     1981 catttctgca gctttgttat aatctccctt gttcaaagct tggacaaatt gctcaactgt
     2041 cttttctcca gcttctactg cttgggtttc ttgatagtgc tggtagtaaa aataaccgcc
     2101 acccgcagct gctattaaga tcactgcgcc ggcaatataa gcgcctgttc gatttttgcc
     2161 gtgcttgtca cttcttttca ttttttcctc cttctcttgc ttgtcattcc tttatttcac
     2221 tatttatacc tgtttattct aacatatgtc tttgaggaaa tccataccac aatccagaag
     2281 cacactttct ggaaggttcg tttggatact accaagtaat ttcccaaccg ctttgggtag
     2341 cttgaatatt gaagttacgt ctttcagctg ctgagagata gctttgatca cttgctgctg
     2401 ccttctgatc ctcccgaagt ctccttcttt gtcttcccga aatcgagcat actggagtaa
     2461 agtattacca tccatcactt gctctccttt gtttatatat acgccatcta aatctagaga
     2521 tttttctgca tcgatcttga ctccaccagg aaacagttca ttaacgcaat cgataaaatc
     2581 ttgaaatgtg atacttgcat aatagggagc ttcaaatttg aaattttcct ttaatgtttg
     2641 atttaataaa tcaactcccc cataagagta agctgcattt atcttattca ttccgtaacc
     2701 tggaatatcg acatatgaat ctctcatgat agagatcagt tttgcttgtt gtgttttctg
     2761 gttgtattgt gcaaccagaa gtgaatccga gcgaccgttt tcgtctttcg aacgagcatc
     2821 tgaaccaatc aaagccacat ttagtgtggt agggatactt ttcggctcta cctttttcac
     2881 agttgaactc tgttttgcag acgaaaatcc atggataaac tcaatcgaaa aatagccaac
     2941 tactaatgta agcagtaaca aaatagataa tatgattttt tgagatactt tcattgcgaa
     3001 ttcctctttc taaaagaacc tctcgcttga aaaataaaag attgtacttt atgataattc
     3061 aaactgagag tttctcagtt tctctttatt gtttttcagg tcgatagagc gccaactctt
     3121 cggcctgtat ctttgaagaa atattcaacg tgattccgat tcctaaagaa aggatcagat
     3181 aacttgttcc tccgtagcta acaaatggca atggcacacc agtcattggc atcagccccg
     3241 cgatacttcc cacgttcatt atcgtctgaa cgaatagcaa agttccgaat cctaaaagaa
     3301 acaatccagc ttgctgattt ttacagcgac tgcttaaaca aaagattctc atgcataatg
     3361 agaacaataa gaaaagcaca cacaatgcac cgattagccc aagctcttct gtaataatcg
     3421 aaaaaataaa atctgtttct gtctctggaa gatacccttt ttttgtgata ctatttccta
     3481 atccccgccc aaatatgcca ccattatata gtgcataaaa cgaatgggtc atttgatagc
     3541 ccgcaccatg actatcgata aaagaatctc ttaaagtcgc gatcctttca taagcatgat
     3601 taaacatctg tggaagccaa cctttatttc ctaaatataa taccccgcct gctgctccaa
     3661 tcagcaatgc agaaaaagta acgattagat agatcccttt tttaaatgga accgccgctg
     3721 cccaaaatat gacccacgca atcgaaagga tcatcaatgc tccagcaatc tttggctgaa
     3781 ataaaatcag aaaagttata cctactgtaa tcaggaatgg tttttttaga tcttttgggc
     3841 tactatttcc gtcacgaaaa aaccagctta aataaaaaat caaaaaaaga tttgccagtt
     3901 cagaaggctg gaattgaatg ccaaacagag agatccaacg ttgtgcgccg ttgacagtga
     3961 caccgaatat ccctattctt actaatacta aaaagaaaat cgataaggct aaaccgtatc
     4021 ctgctatttt agggtgaagt aaatagtgta gtttgactga acgagctaag aagatcacgc
     4081 tccaactcaa aaatatgaaa attagttgac gcaataatag actttttgta ttttcatcgg
     4141 cttgcaataa acgataagaa ctggcactgt atacttctaa aagaccgatc atggacaaag
     4201 tcaaataagg acccaaaatc caccaatcga tcttttttca ccgcatctcc tcctcacaca
     4261 gcaatccact tgtcaggtga ggagcgagtt tgtctcatca aataagtcag tcacaaaaat
     4321 ataaagaaaa ggtcagaaac cttttcgaac aagaattctg acctagcatc atctcatcgt
     4381 ccgagtttta gcactataca acgtaaagaa aacattctta gctattggaa aaagccttaa
     4441 tccaacagtt tctgttttac ccattggcga ctttcgacgt ttctgggcaa cagcctatgt
     4501 ttgtatagga gcctcaccta acaaggttat tactatattc tatttacgag ttcatgatag
     4561 acttttccta atgattaggc aatggaattt gaaatgaaat agtgaaattt aacaatcttt
     4621 aatatttttt agaaaaaaat aacaaataag ttgaaagaat cccgttgcaa attctttcag
     4681 ttgcaccagg attctttgat aattttttag ttttatgtgt ttttttatat gttttttcac
     4741 ttttcggaat aaatatttat ttgttaaagt aattaaacaa tccgctaatc gtttgatctg
     4801 gcatttctat ttccttacca tctaaagtaa cagtagtaac gacaaaatgg tcttgcaatg
     4861 gcggaacttc taagtactgc ccttgcagaa aatcattcac tgtttcatat tcttttttca
     4921 tttcttttga atctgtcgat ggtttatagc ttatttttaa cataactagt gctccttttt
     4981 attcgggacg tgcaatcgtg aaaatttctc ctattgctgc ataatcattt cctgccaaac
     5041 ggcttacagc attcaatttt cttggatcga tccttcctgt cttatcatca tagactgttt
     5101 catttaaatg atatttctgt acttctagca aaaataaatc agctgttact ttatttttat
     5161 tccgtatctc gacatgttca tataaggatg tttcaaaacg aacttttgct tctgtcaatg
     5221 ctggtactga aatgattact gaatcagtta aagtcaagtc agtcaggtac agttcgcttt
     5281 cctcattagg cagattggct gctgttttat ttacttcttc tacgttgtcc tcatcaagga
     5341 tatggataac agcttctctt gtctcaagaa tattctttgc tgtgtctttt actgcatctt
     5401 gttttctctg gatcgccaag gataagatgg gtcgattcga actgacgata ttgaagtaac
     5461 tgaagggtgc gatattcact atatcttttg ctgatttagt cgttactact gctactggtc
     5521 tagggatgac tgaaccaatc agcaacttgt aattttcacg ttctgtcaat tctgaaggac
     5581 tccgttcaaa catggtttcg ccttctttct ataaaattcg ttaaaaagca gatcttgaag
     5641 acaaaatgaa aagtaatgct tttttcatct tcttagaatc tgctttaaac tatctatcaa
     5701 tttttctttt ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg ctttacttgc
     5761 ggcttcgaca tatcttttag tgatcaattg cggacgggtg ataatagatc caactacgac
     5821 tgaaaaagca cctaattcat atgctttctc tacttgttca ggagagtcag tatgtccttc
     5881 cataattact gggatatcga cttgttctat gatttgattc aataaatcaa aatcaggtgt
     5941 ctgcgtcttt cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa gatcaaaacc
     6001 atcttcttgc gcttgaagcg cttcctcaaa agtcgaaata tctgccatag ctaaccgacc
     6061 gtgttcgtga atcatttgga caagttcagc agttgttttt tctgtcggac gtttacgaga
     6121 agttgcatcc agtgcaatga tttctgcttg cgtctccaaa agttcttcca cttctttttc
     6181 tgtcgcagta ataaacactt ctgacccatc gtaatctctt ttgataatcc ctattaccgg
     6241 taaatcaacg attcgcttga tttcattgat atcttctgtc gtatttgctc gaataccaac
     6301 tgctccgcct tcttttgcag ccaaagccat tctccccata ataaaagaac tgtgtagcgg
     6361 ctcgttttcc aaagcttgac aagaaacgac gactccgcct ttgatttgtt ccagtgtatc
     6421 cacaatatct cctccagtaa tgttatataa aaatacagct tcatataaaa atgtaaccct
     6481 tttctattca ggagacaata atgagctatt tctttcagca gacctttctt actaaaagaa
     6541 aattacttca aattcttcgt atacgaccag cttctcttct gtaaattcta tgttcaacgc
     6601 ttctttataa gcatgtttaa aaaactcagc atggactttc cgccaataag ctaatgtccg
     6661 atccccttct ccttctttcc aagcatgctc tttcgtgact tcactaaaag gcaagatggt
     6721 cacatttata tttttgatga tacaaactgg ttcatttttt ccattcagga ccacgtctaa
     6781 ttgaccaact tttggtaaag gctcattctc ttgtttatac agatcgtaac cacttgatgt
     6841 accagtcttt atctttttta caactagttc gctcaactcg tctgccattt tttctgtatc
     6901 cccaaatgaa aagaaaccta atttttctga ttgataacta ggatattttt cttggaactt
     6961 tttgacatat tcttctactt gctgatacat aacatccacc cctttgcttt ttataatgaa
     7021 aaaaccatcc agatactagt ataaacaatt atctggatgg tgagcataat gaattattga
     7081 atattcaaga cacgcagact atcttcaacg atttgttttg gtgttaaatg ataacgttca
     7141 taaagctgcc ctacaggaac gctatccgtg aattctttct ctgcaccaaa atttaatacg
     7201 ttcattgaag aatcaccata ataacgactg atattttcgc caaatcctcc tgacaaacta
     7261 ccatcttcta atgtgatgac taattcgtgt gtttctttta atttatctaa tgtttctacg
     7321 tcattttctg tgatcgttcc tggattgatc aacgttgctt cgattccttt ttttctaaaa
     7381 gcttttactg tttcttttcc taattcaaga aaaccgccta aagctaaaat tgctactttt
     7441 cctccattat ttactgtttg ataggctgga atactataat catttgcgat agctgtacca
     7501 tgttctacac catgtgtagg aattcggatt gctacaggct gcgctgtttg ttcgaacgcc
     7561 caatctagca tagagatcat atcctctttt gttgctggag caagatattt gatgttcggg
     7621 atacttgata agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact agctgagatc
     7681 gttccgccac cgactaaaat aatcactgga ttctcgttga tcgctaaatc atgtgataat
     7741 tgatcatatg cccgttgcaa gaaagtactt gattggaaga cgactggacg tactccctga
     7801 gaagccaaag cagaagcgaa agtgatagag tgctgttcag cgatccccac gtcaaaataa
     7861 tgatctggat gtttttcttg gaatgttttc agtccaaagg cgcctggtat cgctgcattg
     7921 actgcggcaa taggagctgt acttgtttgc attttcttat ccaaataatc catgatgatc
     7981 atattaaatg tttctccaga agttacttgc tttgcctccc cggtggacag atcaaatggt
     8041 acatgccaat gatatttcat tttattatcg attgccggct gataaccgcg acctttttcc
     8101 gtatggacgt gaagtacaat cggatgatca atatctttta cttcttcaaa taaacggatc
     8161 accgtttcca gatcgtttcc attatccacg taacgataat caaatcccat tgctttaaat
     8221 aggttattgc tgcttttacc gttggtttga cgaagctcag ctaagttttt atataaactg
     8281 ccatgattct catcaataga catctcatta tcgtttacta atacgattag attggattgt
     8341 aaaacaccac cattgttcaa tccttcaaaa gctaagccac cacttaggga gccgtcaccg
     8401 ataaatgcta ccacatttcc tgattctttc ttcagatctc tcgctttagc taaacctgtt
     8461 gctaagctga tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt actttcatgt
     8521 tggttggaat atccgctcac ttcacggtaa tgtccttttt caaaaccatt ttttcgacca
     8581 gttaaaagct tatgaccata agactgatgg gaaacatccc aaacgatttt atcctttggt
     8641 gaatcaaata cataatgaaa cgcaatcgtt aattctacta cacctagatt cggaccaacg
     8701 tgcccaccta catcgctgtc cttttgcaag attttttggc gcatctcttc tgccaactgt
     8761 ttcatttcct caacagataa ttttttgaca tctgcggggc tattgattgc atccaataca
     8821 tgtaatactg tactcataat acattctctc ctccgtacta tcttctttta atgaaaatag
     8881 atttgttaca ctttatctat tctactactt agagttgact ctaagtcaat agcattttaa
     8941 aaaaatttca gagaaagctt gttatataag tagatcttcg cacgaatcag gactcaaata
     9001 aaaaccttct acagacaatt aaaaagcttt gtagaaggtc tcctaattaa ggtctcatca
     9061 agaaatatgt ttttttaata ggtgaagttc ctgagcaata tgatacaaat ggatgtttgc
     9121 accttttaat gagattggat agatctttgt cttttcagtt tctaatccat tttgatagct
     9181 agcagcatcg cacaaaacca ctcttttttt cgcacataga cattcatcag gcaattcttc
     9241 taatgaacga aataaaatag gatttatttc agcgatgcct tcttccgcat acaaatgaaa
     9301 ttgggttcga taaaaaaatt gtttgactga actttctgaa acaacaaaaa tttctatttc
     9361 ctgtctcccc gcttgttcac tgttgatcaa agaaagactt ttgacgaatt ttcgaatgaa
     9421 attttcattc catcttagtt ggatttgatt tttctttttc cactcttcca atatttctac
     9481 aatttttccg tacagaagtt gttctttttc tgatagaaga tagatccttt ccggtaaaaa
     9541 tacttgtcca tctgcccaca acgtacggac aaattgtaaa aaggattgct tgaaaataaa
     9601 attttctgca tcgtcgattt cagcgatact aataatctgt aaaatcaatt cattcagtga
     9661 taggtcattg agaacaaaat ttttataaac aacttcgaaa tcttttctta acagctccat
     9721 gttctcattg gtataatttc tactgttgaa caaagaaaaa atataataga catcttcttc
     9781 ttgtaagtct agaattcctt tttgctcttc caatccttga cagactaatt gaaataacgg
     9841 cttcgtttct gcgcttttct tatcttcttc tttcagtcca aggggaaatg cctttcttcc
     9901 gattgcgatc tctatttctc gtatgataaa ggacttttca tcttccgaat atcttctttt
     9961 aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa gcttcttcta catacggatt
    10021 tttattttca tagccttccc aattgacata acgtactaaa gctagcaaga tgttccgcga
    10081 gtcaaattct gggataatga tttctttatt tttcaaataa tcattttctt taaaaaaatc
    10141 caatacgatt tttttgattg tatatacttt actcaaagaa ataaattcaa gatcagccag
    10201 ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa aataagattg
    10261 accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata cgagtttata
    10321 cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg ctagactttc
    10381 tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt gttcgatgtc
    10441 tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata tatttcgctc
    10501 gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat tttatttaat
    10561 catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt caaaaaccaa
    10621 taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc attgtaggta
    10681 gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt caaagatgtc
    10741 agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc tgttaccaaa
    10801 tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag ctgccatcaa
    10861 cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact ctcctgattc
    10921 atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt cttcagtaat
    10981 gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag ctaattttgc
    11041 tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga gtatctccgg
    11101 tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc gattgatatc
    11161 acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag gatcatcaca
    11221 gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt taccggcaat
    11281 caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat gtttgcgatg
    11341 attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg tgttgattgt
    11401 ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg gaacttttga
    11461 catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt gttttgtatc
    11521 caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg ctggaatgta
    11581 atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt gttctaattc
    11641 ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc tggaatttat
    11701 gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa acaatcagaa
    11761 tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca aatgagatac
    11821 aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt acaacttttc
    11881 cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc ttcaaatagc
    11941 ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac atcgctgcaa
    12001 gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag attgttgcaa
    12061 cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct tttgcaaaat
    12121 attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga gaggttaccc
    12181 aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt ttcgcaatcc
    12241 atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc acccctgtat
    12301 taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca aaagtatcta
    12361 aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt ttagggtttt
    12421 ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa ataaaggaaa
    12481 caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc atagacagta
    12541 tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac aagactaatg
    12601 ctggcaacaa gataccagct accaaaccga tccgagcaat caatgaagtg tatttggttc
    12661 cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc aatttcagat
    12721 atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat
    12781 acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac
    12841 cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgccggtt tcccttagtt
    12901 aaaaatatta ctcttgttga ttactcaact cttccaataa atcggtaata tatctcaatt
    12961 tcctgtattc tttcgttatc ctcattcgtt gttgcttcat gtatgaggat tttttctatc
    13021 atggcattta ataatggtgc tgtcagttcc tttggtacgg aatattcctt gattaactca
    13081 atccactttt cagcacctat catatcctgt tccattttac ttagttcttc tcttaggctt
    13141 gttatctgct gttccagttc tatctgttct ttttggtact tgctggacaa catcacaaaa
    13201 tttcgctctg ttatcttctc acaggctcta tcttcataca ttttcgcaaa taaacggtca
    13261 atctcatttt gtctgttctc ggctttcttc aatgtacttg cctttttctt cttttcccgt
    13321 attctctctg cattgccagc tttctgtatc ttgttcaata ccttttcttc gtcctgctgt
    13381 actgccttag cccaatactg caagcgttcc aatacggctt gatacagcac atcataacga
    13441 atgtagtgca tagaacaatt acctttgcca aactgcccgt agtaactgca agcataataa
    13501 ctgtaaggcg ttttatttgt cttattcgtt ccaaatctca tagaccaacc acaatccgca
    13561 cacttgacaa gccctgcaaa aatcggcgtt gccttttcct ttgtctgtct gcgtcttgat
    13621 tttatctgct cctgcacacg atagaacact tccttgtcaa taatcggctc gtgagtgttt
    13681 tctactcgaa accattcact ttcgggctta cgcactttct ttttgctctt gaacgatacc
    13741 gtagactgca tattgtgaac actgtttccg atataaacct cacttttcaa tatcgccttg
    13801 acatgagcaa tcgtccactc ataacgctta ctttcgggtt ttccctcaaa gatatgtgca
    13861 aaagtaccgt atcttgtaaa atttagccat gacgctgtcg gaactttttc tgctcttaac
    13921 tgctttgtga tttttgcact tccgtaacct tgataggcta ggtaaaagat tttttcaata
    13981 atccattttg tttcatcatc aaccaacagt tttcctttga tgtcagggtg tttcttatat
    14041 cctaacggag cataagcacc gtaatgagcc ccctccgcaa actttgtctt aaatgctgat
    14101 ttgactttac ggcttgtatc tcttgccacc cattcattga tgatgttctt aaatggtgca
    14161 atctcacttt cgcctttttc gctgtctaca ccgtcatcaa tcgctatgta gcggacatta
    14221 tgactaggga aatacaattc tgtatattgt cctgtcataa tataatttct gccaagacgg
    14281 gaaaggtctt tcgttacaac acagttgatt tttcctgcct ctatatcctc aatcattctt
    14341 tgaaaactcg gtctgtcaaa atttgttccc gaccagccgt catcaatata ttcatcaatg
    14401 acattcaaat gatgttcttt tgcatataaa cgcaacatac ttctctgtgt ggtaatgctg
    14461 gaactttcgc cctgtaattc atcatctcgg cttaacctaa gataaagtgc agtattgtaa
    14521 atctgttgtt tcattgtcag ttatcctcct ttataactaa cccgtgttta caatacctgc
    14581 ttgcaagtaa agtataacac gggtactttt tgtcattcaa tctatttctt acaactgcac
    14641 cgattttatg cgattgtctg ctttgctttt tccagcatga catcagttag aagttcttcc
    14701 attgttttgc ccttttcgga aaaatgctct tttactacaa tctttgtctt tcctctgttg
    14761 ccaatgccac acttgccatt tttcttttga attgttttta cagcattatc aataacacat
    14821 accccctttc tgtaaaatct ttctaagcaa ttcaacggct tttgtcatag cccacaggag
    14881 ttccacctct gcatagttct tatgtagccg tacccattgc gtgcgacgct cttaatgctc
    14941 aagctgtggc tgtacgggag tatcattatc tgacagtaca ggtcatggcg ataaaagagg
    15001 acaaatcttt tacaagaaca caaataggtt ttcgctcgct cttgcagggg aaaggtcgtg
    15061 gcgtatttgt ttcaacggct agctgtctgt caaacgtatt gaatagcttt attcagttgt
    15121 caaagaacga ttgaaagaaa aaaatcgtct ttcctatata tcgcgttgga aaagaggaga
    15181 aacgtaacca aaatccaaaa ctttttccat tattagtaca aatggaacgg ttcattttgg
    15241 caatcaacgg ttgattttct cctttttcac tactatctgt acagcaaacg tccatttgct
    15301 aagtttaaat ggaaaaattt taacttcttt tcatgcacca tatagggata gaaaatgtca
    15361 ttttgctaag ttgatgatga aaaaagaata ttttcctttt cacaccctaa ctacccaaac
    15421 cataccgtta ctgccaacaa tagaatgaaa tattttcttt gcaccatata gggaacggaa
    15481 accttaaatt gttaagttga gaacaaagta ttttcatctt ttcacatcaa cactcaactg
    15541 ctaatgcctg tttgaataca aaatgatgat gtttttcctc ttttcacaga agactgtcca
    15601 ctaatcacga atttccgtca ttttttcaaa acttttttct tctttcactt catataggga
    15661 cagcaagccc cgatttaatg acctattttc aaaaaaagtt tagatttcct tttctcgcac
    15721 catatctgta cgcaagtagc cattttgttg cagatttaat aaaaaattta tttttcttct
    15781 ttcacttcac atagggaatg aaaacagctc tttgctgact aaatctgaaa tttttcaatt
    15841 acattaaaaa ataccgtcat tctcttgtgg aacagcggta tctatacgtt cttctatggt
    15901 tgatttctct aattgtaata aatgtttatg cttgtctttt agttctgcct gctcaatcat
    15961 atctttcagt atcgttgtac gattttctct gtctaatgcc tgtaccattt ttatggttgg
    16021 tgcgacttgt ctttgtagcc aatgcaacgc tttttgtaaa gtgtaaggct ctggctttgt
    16081 ggttaagcgt atcggctctc tgttttcccc tacaaaccat gcccaatcat catttgttat
    16141 ccattgactt tttggcttgt cgtcctctct gtcaacaaag cggacataat gattgatgat
    16201 agaaaaagcg gtgcgttcag cgtcccgata ggtcagtaaa tctacaaccg cataataggc
    16261 tcgttcgttc ttcatgcgta tttcaaaacg gttcttaatc tccgtatctt caatttctgt
    16321 gccattcttg acatactgct cataattttt ctgataagca cacatatata attcgctact
    16381 tgttgaaccg agatataagg tttctcctgt attctttggt aaatcgctac cgcatttttc
    16441 tgtaccgctg taatctttct gcatacggaa gtaggacata cattcgccag ccttgtattt
    16501 ttcctttaac tttggaatat ccaatattcc tgctttatca ttgatagcca aatcaagccg
    16561 tttcatcaca ccaccagccg tcatacaatc cagcatgaag tcataccatg agcgttcctg
    16621 tgccagcaga taacattcca tttgccgaca gcctttgcct ttcagttcca ataaaacacc
    16681 taaatgctcc tgcatggaac acatgatatt gatgtcgcca agtacatatt tgctttcata
    16741 tccgtatttt ccataatctt cataaagcat gtaattagat ttcagttgta atacatcacg
    16801 gataatcgcc aatgcgtccg tagtaggaaa gcggacacga aagtaatcaa tcaataagaa
    16861 tagtggttct tgcggattga aacgctcaat ctgtttttct atgatttctc ttaattcgtc
    16921 attcaatggc tgttttcctt tttcaatgcg gttgtaatat tcacggctga taccacaggc
    16981 aacagcaagc cttgtttgtg ttactccgta ttcctcacgc ttttgtctta gttcttcaat
    17041 aaagttttta tcattcattc ttcaattcct ccaatcaaaa aaggcaacta ccataattga
    17101 taattgcctg tcattaaaaa tctgtataga aaaagcagtc aatcctaaga ctaactgctt
    17161 tgtgtgtatg gatataaaat tgcttctaat cttgaatagg tatccaaatt tcaataactg
    17221 aattatcact attccaatga aaacgataat cgtatttttc aaagtgtagg ataatatctt
    17281 ttataggaat atacgatgta ctaggaataa tttcttcata aatcttgcca taagtttcat
    17341 atattttatc catagcacca atatgctcca caaccaaata tttataagac ggtatttctt
    17401 tatacagaaa gccctctggg gtaatggttc tttggggaat agaacagaaa taataaagtt
    17461 ttccatttct tctttccata aaagcatatt ttacccaatt tcctgattgt gaaagatacg
    17521 cttttttcaa actactgtta aactttctcc aaaactgtgt actaatctga atattctttt
    17581 tttgataatt agtcagttct acttcctgcc caataactgc aaatgcgttt aattccttta
    17641 tagaaaaatt tatcatttta ccttgtgagc agtgataacg gtataactgt acgcatttac
    17701 cgttataata caattatcaa tcgttatata ccagtttttt ccttttcttg tgatttccgc
    17761 attagaaagg ctaatttttt ctttgcacca ttcaattaca ttttctacat tcaaagataa
    17821 gttcttttta attctaataa cgccaagttc agttgtgtga agtcttccta agttttccag
    17881 taattcattt tccatatttt gatacctcat ttttcagcaa atttatttgt atgttttata
    17941 ccccaattat aacattctca tatcagcact acaataaaaa taattaagca ccaataatct
    18001 tattgaacat ctccaaccgt aaatctttca caccatcagt gataaacact gagctgacag
    18061 caattacgac aactaccata attgcctgca cttaaatttc tgcaaaagaa aacgacaatc
    18121 aatttcttat tttttgaaat caactgtctt tctcatattt actattcaat tttcatagtg
    18181 atataggtac aatttatatc actactgtaa aaatggggtt ttgccttata aatacagcgt
    18241 tttccgcttg ttagtgtata gtgtttcttg ttttttgtgc cccccttgtt agataacggg
    18301 ggctttgaac ggctcgcaag ctcgccgaac cgccgaagcg aaccgccccc ttgcaagcat
    18361 ggcggaaaaa tcttaaggat tttcccgcca acgtggtcgg atcgctccgt cggtttaaca
    18421 gcttttctgt ttctctctag ttatctacgc atataaatca aatcggttat tccgttataa
    18481 gtctgtgttt tacaaagcct taactttatt tccctgtcta tgttgcccaa aagacgaacc
    18541 ccatttccta acagagtagg aataacagaa atgtaatatt tatcaatcat atttttgctt
    18601 accaactgct gaacaagatt tgctccgcca caaatccaaa tatcctttcc tgcttcttgt
    18661 tttagccgtt ccaataataa aacaggattt tcacttgtaa aatgaatttg ttgggaagaa
    18721 ttgcactctt tatgtgttaa tacataagtt gtaaatttat tatataccca ttcttgcgga
    18781 gaaagttcag taacaacctg atgagaagta ttccacccca ttaagacagt atctatatct
    18841 ttggtaaatt ctgtataagt gtcgatgttt tcattgtcat ttccatgacc atttagccaa
    18901 gctactccac cattaccgtc cgcaatatat ccgtcaaggc tcatggcaat aaataaaact
    18961 acttttctca ttttttctct tttataaata caaacaggaa tttatttctc ttttttactc
    19021 ataagggaac aactgattgc tacaaataaa cccattaaaa tccatggaaa tgctgctctt
    19081 ataaaatcac taaccatact ttattcctcc ttaaattcca atttccttag ttctacaaac
    19141 tgaaattttt gcctatgaat gtatcttgtt ctttatacgc aaacaggctt ctgtgtgcat
    19201 aatccaatgt cttgaaaacg gcatttgaat tagtccacaa atatctttat tacaccaata
    19261 atcaatcagc caaattgctt tttcattgtc gtttactaca ttcaacgatt ctaagtatcc
    19321 ttttctttct ttcggtattt ttcttttcaa ttcatcataa gataaccgtt caagcatttt
    19381 ctcagtgctt tcccatactt cgaaaatata taaatataat tcttccagat taagctgttt
    19441 tgaaaagtct gcaatctgct gtttcatgag ttcatttcct gttgtaatga taggtgaatt
    19501 gatacgctct tgataattgc ctgcaaaaaa tacttgttca tcttcattta tcactgtatg
    19561 cacaactata tcttcaatgc ggaaaatatg ccaaatagaa taagcaatcg tcttactatg
    19621 ataaccatca gcgtttatga aggggattga gttaaaatct tctctgcata attcttcttt
    19681 gaaagatact aaggtttgta ttaactgact tcgtaaagta agtaaagtat caatacccct
    19741 cttataagta tctttctttt ttatttgtgc ctgcattgtt ttattaagtt cagaccattc
    19801 cttattcata ttcaatcctc tctcaaattc tgattttatc gccccataca aactggaatt
    19861 tgtcttacaa caatttatat atgcataaaa agcaatatat ttatgaaaac accctaaatc
    19921 caaaatttac tcaattcatt ttttccttac ataaaatgtt ctcgtatatt tcacttcctc
    19981 accaagtgtt tcccctatca aaacttcaaa atcattgata tcaaattctg gaaagaatgt
    20041 atctccatct tcaatattta aatctacttc tgtgatatac attttatcta ctatttgcaa
    20101 agcttcctta aatagtccat atccaccaga tatgtataca tctcgtcctt tagccaataa
    20161 taatgcatct tctaatgatt taactgaaac taaattatct ccttgatact ctgttgtggt
    20221 ggaaacaaca atattcattc tattaggcaa cggatgaccg atttcttcat aagactttcg
    20281 ccccataata accacattac ccgttgttaa ctctctaaat tgcttttgtt ctccctttat
    20341 tttccatggt atattaccat tcttgcctat aacattattc tttgacctcg caacaatcaa
    20401 acctatcatc tatttcatcc tccataatat gatgtcgtac ctgtgattcc atcaagtgca
    20461 ccacatctta acaaactcaa aattatctcg aactaaccag ctcgacaaat tccttttttg
    20521 tcttcatctg cattatatca tattgttata agcattgcca tttcttttct gcttttgaaa
    20581 agttacttat cagcctatgt tttgtaaagg gtgatattca gcatttctca cgcaacaagc
    20641 ccttgacaaa ccatagacta ataagtttta aggtaatcaa gcagaaatga aatagtctgc
    20701 tgtttcacgg caacaaaaaa gagataccaa cacacttatg tagtatctct tagtctagta
    20761 gcaatattct tttgtttgca agcaggtaaa catgggctgt ttcttaatat ctttttgttt
    20821 gggaaacacc tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag
    20881 ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg
    20941 cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa
    21001 agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt
    21061 tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt
    21121 cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg
    21181 ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga
    21241 agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa
    21301 tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt
    21361 gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca
    21421 gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa
    21481 cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca
    21541 gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt
    21601 gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc
    21661 ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct
    21721 ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga
    21781 ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc
    21841 agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt
    21901 ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca
    21961 tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga
    22021 agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat
    22081 cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg
    22141 agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa
    22201 aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg
    22261 tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag
    22321 tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat
    22381 aaaagacaga caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa
    22441 taaagacaaa aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt
    22501 atttttaaag atattttttc ataaggaata aagaatactt catataaaat atataaaaaa
    22561 gaaccgcagc gagtgtcgct acggttctca aatttgttct tcatttcaac ataagttttt
    22621 cttcaatttg cttataattt acgtgcaaag aatgaaacga ctaatactaa aatgattgca
    22681 ccgatcaatg atggaatgat agccattcct gcaattgttg gtccccaagt tcctagtaaa
    22741 gcttgtccaa ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat ccccattgat
    22801 gatcctttat tcgtgattgc tcctgcaatt gctccaatga taccgccgac gattaatgcc
    22861 caaataaatc ccattttaaa ttcctcctaa ctgacttctt acttcttatt gtacacgtgg
    22921 ttcgttattt tctttgactt tttgtgcacc tttgcctaca gcagattttg ctttatctgt
    22981 ttggcgtgaa gcaaattgtc ctgtggattt tgctgcttct gttactttgt cttgtactgt
    23041 ttcttgatct ttttggtatt cttcttgtgt tttgatgtct gcaacattca cattcacttc
    23101 gataacatct aaatgtgtca tattgtttac ttcagtactg atcaattctt tgatttggtt
    23161 gtaaatatct tcgatgtctt taccgtattc tacgatcaca tctagatcca cagctacttg
    23221 tttcttacct acttctgtat tgatacctgc tgttacgtta tctgtgttga ctaatttttc
    23281 agccatattt gagaaaaatc cgccatctaa tgtcaataga ccatctacat ctttcaatgc
    23341 ataaccgatg attttttgga tgactttgtc ttcataagtc aattcaccat tcatcatgcg
    23401 gttttctcct ttttcatttg ccaaaactga tccatttgtt gtattcatgt ttttgttttc
    23461 catctttgtt tcctcctatg atttaaaaaa ttgatcaact attcctgact gtttcaaata
    23521 aaaaccgatt cctgcgccaa ttgctgtcat gattatgatc aacaatgtct tgaaaaatcc
    23581 aatcgttaac cataaaacag ctagcagtag tccaagaccc ccaaagataa tagggagctt
    23641 gtatgctgaa aatagttctt tcattatttg gccctcctat tccactcgag agtgcttgta
    23701 gtctcttgta tcttcttgtt cttcataacc gctgtatgtg actgccactt tgaccgtttc
    23761 ttttgttccg agtactttag ttacttcttc ttcgatatct tgcatcaata cacctgtttt
    23821 ccctactaat gaagaagttc gtttcaagtc tccgttgaca tggatatgga ttcgattttt
    23881 cgttgaacgt atccgtactt taggtgaatg aatgaactct tcttcgtgta aatgggaacg
    23941 taccaaccct tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc gtttcataac
    24001 gaaagttcct cttgactttg gatagaaaag aacgactagc aagatgataa tagcaagaac
    24061 ggcaaatact acagctgccc agaaaatata ttgttgcatg tagtaattcg ttaaagtaaa
    24121 gaagcgaaat cgttccaatt gttcaggcat aatatacggc gcattgatca acgcaatcgc
    24181 tagaaatact gaaagtagca gtaaactcat gataacaccg attattttta accccttatt
    24241 cattttccac ctcctataaa cttattttta ccctgtaaat aactctaccg acaatcacag
    24301 tatagatagc gaacagttag catagcatca gtaaataaag cgctaccaaa cattgctgga
    24361 tcataacggc gttttattgc cagtctgata ctgaaataac aattgcttac ttattctcta
    24421 tttacttttg attgctggct atacttaaag gatattattc aagattaaaa attgcaaaga
    24481 ttatgctcag ttattattac aagattctat ttatttatta aattttttcg gagaattaaa
    24541 aaatacaata aataacttta gttactttaa tgcgttctta ctttattttt atattatcat
    24601 ttttcttatt cacttctttg gtttatacta ttcttataaa taaaaacgag gtatgtccta
    24661 tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc
    24721 taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat
    24781 ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg
    24841 ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt
    24901 tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca
    24961 tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg
    25021 attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca
    25081 aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga
    25141 atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct
    25201 cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa
    25261 actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta
    25321 caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt ttttataaac
    25381 gatatatttt tattgttttt cgataaaaaa tagttgtttt acaataatta ttctgctata
    25441 ttttagctat caaagataaa agagggataa aaatgaaatt taaaacattg ctattaaaat
    25501 ttgtcgtttt aggtataagt gccgtattta ttttgttcgg cgtacttttt tatactcaga
    25561 tcgctacaag cgaaaaagga ttcaattttg attggcagac cacattattt gtttctgtca
    25621 tttactttgc ccttctccta gggatcgtta ttgcttattt cttattgaaa ctattgaaaa
    25681 aaatcgaaga taaccaagct ttttccactc aaagcttaaa catactaaaa aaaatcgaac
    25741 gaaccatttt gtttattggt ttcgcctttt ttggcttact tcctaaattt atgaaagtag
    25801 cggatttaga cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt
    25861 ttgcagtata cacattaaca agcgtgctgg aaaaactgct tgaacatgcg attttactta
    25921 agacagatca tgatttgaca gtgtgaggtg cgacatggca attatcgtta atttagatgt
    25981 agtattagct caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtgg gcattacgat
    26041 ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc atccgttttt ccactctcga
    26101 aaaaatctgc caagttttgg agtgtcaacc aggtgatttg ttgacctata aaaatgactc
    26161 gaatactata ccagaagaat aaaataaagt agaacaaaca gtaaataaaa aataatctga
    26221 atgatagatg tgtgagactt ttagaagtta tttacgtcta ttattcagat tttttatttc
    26281 gattgaatac cgttttatct ataaataaga tatgtactga ctcatacaaa gggggccttt
    26341 tcttctccat gtactatcac ttttcctgat acaactggtt tcctgttacg ttgtagttgg
    26401 atctgctttt agtataatac tttattcatt ccaccagact gatagcagtt tgagcagtct
    26461 aaataatcat gtagtattac aggccattct gtttcatacc ttgaagtgct taaaacttca
    26521 gctaatggcc cattgtgtta actcttactc cattcttttt tagtaagctc ccatatatac
    26581 tgtaaagcct tctatatatt atgtcatgtt gctttttaac atttactatg tagttttgat
    26641 aaaatgattt gaataacagt gatggacaat tcttttttat ttttaacatt attttgataa
    26701 taatg
//
LOCUS       DACEJZ010000034        26351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEJZ010000034 DACEJZ010000000
VERSION     DACEJZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.034"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW656_001316"
     CDS             complement(98..1228)
                     /locus_tag="IW656_001316"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0837029.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IW656_001317"
     CDS             complement(1877..3079)
                     /locus_tag="IW656_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0837030.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IW656_001318"
     CDS             complement(3139..3342)
                     /locus_tag="IW656_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0837031.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IW656_001319"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IW656_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IW656_001320"
     CDS             4879..5691
                     /locus_tag="IW656_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837032.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IW656_001321"
     CDS             complement(6715..6867)
                     /locus_tag="IW656_001321"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837033.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IW656_001322"
     CDS             complement(7058..7936)
                     /locus_tag="IW656_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0837034.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IW656_001323"
     CDS             complement(7975..9093)
                     /locus_tag="IW656_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0837035.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IW656_001324"
     CDS             complement(9090..11189)
                     /locus_tag="IW656_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0837036.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IW656_001325"
     CDS             complement(11240..12061)
                     /locus_tag="IW656_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0837037.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IW656_001326"
     CDS             complement(12058..12867)
                     /locus_tag="IW656_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0837038.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IW656_001327"
     CDS             complement(12888..13382)
                     /locus_tag="IW656_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0837039.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IW656_001328"
     CDS             complement(13394..13882)
                     /locus_tag="IW656_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0837040.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13958..16654)
                     /locus_tag="IW656_001329"
     CDS             complement(13958..16654)
                     /locus_tag="IW656_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0837041.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17082..18155)
                     /locus_tag="IW656_001330"
     CDS             complement(17082..18155)
                     /locus_tag="IW656_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0837042.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18152..18979)
                     /locus_tag="IW656_001331"
     CDS             complement(18152..18979)
                     /locus_tag="IW656_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837043.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18980..19786)
                     /locus_tag="IW656_001332"
     CDS             complement(18980..19786)
                     /locus_tag="IW656_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837044.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19798..20883)
                     /locus_tag="IW656_001333"
     CDS             complement(19798..20883)
                     /locus_tag="IW656_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837045.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20900..21442)
                     /locus_tag="IW656_001334"
     CDS             complement(20900..21442)
                     /locus_tag="IW656_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837046.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21700..22689
                     /locus_tag="IW656_001335"
     CDS             21700..22689
                     /locus_tag="IW656_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0837047.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22686..23423
                     /locus_tag="IW656_001336"
     CDS             22686..23423
                     /locus_tag="IW656_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0837048.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23420..23971
                     /gene="coaC"
                     /locus_tag="IW656_001337"
     CDS             23420..23971
                     /gene="coaC"
                     /locus_tag="IW656_001337"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0837049.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23987..24619
                     /locus_tag="IW656_001338"
     CDS             23987..24619
                     /locus_tag="IW656_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0837050.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24704..25351
                     /locus_tag="IW656_001339"
     CDS             24704..25351
                     /locus_tag="IW656_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0837051.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25356..26339
                     /locus_tag="IW656_001340"
     CDS             25356..26339
                     /locus_tag="IW656_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0837052.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8059 a         5570 c         4465 g         8257 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aacagattag acactttttt agacacttta tttttgaata tacggtccta
    13981 ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat ttcatctcca
    14041 ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt ttcaaatcat
    14101 aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc atcaatgccg
    14161 tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag taattttcat
    14221 atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga aagttcaaac
    14281 ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag cggttaaatt
    14341 ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta
    14401 agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga
    14461 tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat tcacgtagtt
    14521 ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga cgaaaaacat
    14581 ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg ttgctacttt
    14641 gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca tcctccgcaa
    14701 tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca atggaagtcg
    14761 tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt
    14821 gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact tgccgtttca
    14881 cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc atatccaacg
    14941 cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg ctagccgtac
    15001 tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca taatcagata
    15061 aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat
    15121 gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct aatgtacgat
    15181 ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg ctaataattt
    15241 ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat acccagacct
    15301 ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc ttatcaatgt
    15361 gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg aaattacctt
    15421 catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt ttgattcgat
    15481 ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag ctttctactt
    15541 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
    15601 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
    15661 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
    15721 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
    15781 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
    15841 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
    15901 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
    15961 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
    16021 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
    16081 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
    16141 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
    16201 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
    16261 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
    16321 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
    16381 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
    16441 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
    16501 cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt
    16561 tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa taaactatcg
    16621 gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca caaatagtcg
    16681 aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt ttatctaata
    16741 tgatttttat gttttttcat agctacaaat attgaactgg attcatatat tagttttatt
    16801 aatagattac agaagggtta agtatatttg catagtttcc aattacttta tccttctgta
    16861 actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat ggttttgtcc
    16921 acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt
    16981 tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat ttttagctct
    17041 ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct atacatcttg
    17101 aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc tttgtagact
    17161 tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga aatgtctttt
    17221 ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc tgcattttgt
    17281 gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc tttatttttc
    17341 acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg tgggatcaca
    17401 taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa tgtcactgcc
    17461 acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat tgctttgaca
    17521 tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat attcttacta
    17581 ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc accgtcaatc
    17641 agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt aatttgattt
    17701 gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata tggaatcgaa
    17761 tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt atttaatcct
    17821 ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt ttggatcata
    17881 tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt tgtgaacatc
    17941 gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt ttcaaatttc
    18001 gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac ctttgctcca
    18061 ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc aataatgatg
    18121 agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta
    18181 ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag tagtatccaa
    18241 tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt tctaaactga
    18301 ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca tttcctgtga
    18361 cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa tatccagcta
    18421 tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa ttattagcac
    18481 caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt ggcaagacca
    18541 tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc tgaaaaccta
    18601 atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata acatcaggag
    18661 agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt
    18721 aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca gataagaaag
    18781 ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa acagcttcat
    18841 aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg aaggaataaa
    18901 aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa taaagatacg
    18961 accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct gtcaggagca
    19021 tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc ccccagtttt
    19081 gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga tttccgccaa
    19141 tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg acaccacttt
    19201 tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc
    19261 ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca ttgaaaattg
    19321 gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg aagtctgtga
    19381 ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg ctgccgtgca
    19441 cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat gtaggcaaaa
    19501 taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt
    19561 aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa ttgatcgtca
    19621 tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg aattgcccat
    19681 tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg aataaaccga
    19741 gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt gtccccccta
    19801 ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga aacgcatgac
    19861 atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag cttttttcgt
    19921 tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat aatggacacc
    19981 acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg tcgtgatcgt
    20041 caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc cgccatcgac
    20101 acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta caccgtccac
    20161 gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat cataaatatc
    20221 tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct catcactcat
    20281 tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta atcgttgttg
    20341 cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg ccgataacgg
    20401 ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg ctacacgctg
    20461 tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa gctggaccat
    20521 acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct tgatcttcaa
    20581 tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat cttggaagac
    20641 tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt caaaatagat
    20701 gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg ttttaccaca
    20761 gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac ttactttttt
    20821 taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga ttggtttccc
    20881 cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat acttttgttc
    20941 gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag atcgactgtc
    21001 ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct tctaatacaa
    21061 aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca ccttcaaaag
    21121 tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat tccaattcat
    21181 acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc acttgcagat
    21241 tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca agaatagaaa
    21301 agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata tacccttttg
    21361 acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg cgcaaatttc
    21421 ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat agtaaacttc
    21481 ctgtttacta ccactcgtat tagtatacga aatttattga aaaaggcaac cctttgtaaa
    21541 attttttttg ctgaatattt ctttatcttt tggaataaat tctagttgtt tgattcttta
    21601 tttttcacct aatttttgtc taaatttacg gaagaacaag aataaaagag taggctttat
    21661 ggtatgataa aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt
    21721 caggtaataa aggaacctgt tttccataca gcaatcagtg atctgccatt tactgagttg
    21781 gctgaaaatg aagtcttggt aaaaattgcc tattctgatg tcaattataa agatgcattg
    21841 gcaatgagcg agtctggtca ggtgatacga acatatccga tgactccagg gattgattta
    21901 tccggaactg tcgtccaaag taacaatcca cgtttttcaa aagaagatct tgtacttgct
    21961 actggttttg ggcttggcgt cacccatcca ggcggttata gccaatatca aaaagtacca
    22021 ggagactggt tagttcctct accaaaaaat atgacgctgc gtcaagcgat gatccttggt
    22081 acagcagggt ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc
    22141 gataaaaaag ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc gatagccctg
    22201 ttacataaac taggattcca gtcaatcatt gccttttcca gaaaagaaga gtctgtcaca
    22261 tggctaaaaa gtctaggtgc tttgcaagtc gtacgacctg atgaatttct gccagagaca
    22321 acaaaagctt taggaaaaca gcaaatcgac tatgtcatcg acacagtagg tggcgagcag
    22381 ctaagcagtc ttcttccatt gatctcttat aacggtgcag tagctttatg tggcaatgct
    22441 gggggaatca aactaaacgc gactgtcttg ccttttattc tgcgaaacat ccagttgatt
    22501 ggaattgatt ctgtcaatgt accaatagat caacgtcttt ctttatggca acagatggcg
    22561 gatttgcaga ttgcagatga actggtcgtc caagagatta ctctggatca attgcctgaa
    22621 acagcatcta aactacttgc tggaacacac caaggaagaa cactagtaaa tgtaggtgac
    22681 cataaatgaa gattctaatc acagctggag gaacgactga aaaaatcgat caagtacgtg
    22741 ctattacaaa tcattcgaca ggacgattag ggcaagcatt agctgattat ttagctgcca
    22801 atccggatac cacaatcgat tacgtgacta cgaaacaagc actgaaaccg aaaagacact
    22861 ccaatataac gatttatacg atcgaatctg cactggactt atttttacaa ttagaagaac
    22921 taacgaaaaa agaacactac gatgcgatta tccacagtat ggcagtcagt gattttactc
    22981 ctgcttttag tttttcagaa gaacagctgg caaaaaacct acccacttct tcaacgcaag
    23041 aagaactgga caattggttt gcggaaaatg aacagacaga taccactgtt tcaaagatct
    23101 cttctaacac cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc atttcttatc
    23161 tacgtgaatg gcagccaaaa gcaaaaatca ttggtttcaa actattagta gatgtaccaa
    23221 aggaatctct tctggcagtc gcaaaaaata gtctgataaa taacaaaact gattttattt
    23281 tcgcaaatga tttgacggaa atccatggcg agacacacca cggctatctc ttatctaaag
    23341 atggaacagt ggaagaagct caaagtaaat ctgagattgc tgcattgatt actgagaaaa
    23401 ttagactgga ggaatcaaaa tgaaaaagat cttactaggc gttactggaa gtatttctgc
    23461 ttacaaaagc gctgatctta caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat
    23521 tatgtctaaa agcagcacac attttatcac gccattaacc ttgcaatctc tgtcgaagcg
    23581 cgcagtccat acagacgtca tgcaagaatc aaatccgcag gtaatcaatc atatcgaact
    23641 agcaaaacaa gctgacttgt ttctcatcgc gcctgctaca gcaaacatta tcggcaagct
    23701 agcaaacggc attgcagacg atcttctttc aacagtcgca atggcgcttc cagtgggaac
    23761 accaaaattg attgcccctg ccatgaacac gaatatgtat caaaacccga tcactcagcg
    23821 taatctggaa acattgagat ctgtcggcta tcaggaaatt gaaccaagag aatctctttt
    23881 agcttgtgga gattttggaa aaggtgcctt agctgatatt cagacgatca tcgaacaggt
    23941 catgctgact cttaatgaaa gaaatgctta gaaaaaagga gtacttatga aaaatacaaa
    24001 aaactttaca ttaactgcga tgtttttagc tattttgatt ttattagctg ttacgccttt
    24061 aggttttatc cctatcggtc caatcaatgc aacaaccatg catattcctg taatcattgc
    24121 ttcgatcgtt ttaggaccaa gactaggtgc ttttcttggc ggtacatttg gactgatcag
    24181 catgatccgt agcacagtta tccaaacacc tttgtctttt gtcttttctc cgtttatccc
    24241 agtcatcgga acagaacatg gaagctggaa agccttgctg atcgcgttta ttcctcgcat
    24301 cttgattggg gtggttcctt atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa
    24361 agcagattcc gtttctttat tcttagctgg actttccggt tcattgacca atacgatttt
    24421 agtgatgaat atgatctact ttttattcca gcaagattat gccaaagtca tcggaacaaa
    24481 tttaaatgct gtttattcag ctattctagc ggttattttt acaagtggta tacctgaagg
    24541 aatcgtcgca ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat acttcaagca
    24601 cccttctctt actaactgat agtcatcgta gccgatctct gatttttttc tcttttggtg
    24661 ttactattga atggaaagat ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt
    24721 aggtgcaaac ggacaaattg gccgacacct gataaaagat ttagctccaa gttcccatga
    24781 aatttttgca ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc
    24841 gtatgtctct tttgatttaa cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat
    24901 cgatgtattg atttttgctg ccggctctca aggaaagaat cttctgcaag tagacttgga
    24961 tggcgcaatc aaaacagtga tcgcagctga aaatgcgcat gtctctcgct atctgatggt
    25021 cagtgctgtt tatgcagatg aaccagcaaa atggcctgag tccatgacag attattatat
    25081 tactaaacat tatgcagatg aatggctgaa acgaactaac ttagattttg tcattcttca
    25141 accagtcaca ttgactaacg atgaagaagt tacttccatt caattaacta agcctaatga
    25201 gaaggcatca aaaacgatca ctcgctctac agttgctgct gtacttgccg ctctagtgga
    25261 agaaacggat atttctcgta cgactctagt actttctgaa ggtagcaaag agttgaatac
    25321 agcattccaa gaatgggcaa aggaggaata gatccatgcg tgccatttca atcaagcatc
    25381 ctggttctgt agatgtatta gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg
    25441 aattactgat caaagtccat acagcagctg tcaatcgaac agatattatg aaaagagaat
    25501 cagtctcttt gaaacctccc taccctattt tgggagtcga agtagctgga gaggtagttg
    25561 agaataaaag tgatatctcc acttttactc ctgggacaaa agtatacgga ttagtcaatc
    25621 taggtggtta tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata
    25681 cgctagacta tgtttctgct gctggaatct cagaagtttt tttgactgct tatcaaacac
    25741 tttactggct tggaaaatta caagaaggcg aaactgtctt gatccatgca ggagcaagtg
    25801 gtgtgggaac agcagcgatc caattagcta gacagctttc tcacgcgaaa atcatcgtta
    25861 ctgcaggatc agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat gaattgatca
    25921 actataaaaa acaagacttt caagaagagg tacgaaaaat cactgaaggt cggggagtcg
    25981 atgttatttt ggatttcatc ggcgcttctt actgggaaaa gaatttagct tctatagccg
    26041 ttgatggacg ttgggtcttg attggtatgc taggtgggac catcgtgcct gagatagacc
    26101 ttggtgtatt gattgggaaa cgtgtccaac tgcttggtac attattgaca cctagaagtg
    26161 atcagtataa agcagagctc gcacaaactt ttatgcaaaa agctgggcct tttctagaaa
    26221 atggcaagat tcggccaatc attgaccgta cattccaatt cagcgcagtc aaacaagcgc
    26281 atgaatatat ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata actgaataaa
    26341 atcatgctga t
//
LOCUS       DACEJZ010000035        25599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEJZ010000035 DACEJZ010000000
VERSION     DACEJZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.035"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="IW656_001341"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="IW656_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0837053.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="IW656_001342"
     CDS             809..1243
                     /locus_tag="IW656_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0837054.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="IW656_001343"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="IW656_001343"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0837055.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="IW656_001344"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="IW656_001344"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0837056.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6193..8424
                     /gene="pflB"
                     /locus_tag="IW656_001345"
     CDS             6193..8424
                     /gene="pflB"
                     /locus_tag="IW656_001345"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0837057.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8668..9429
                     /gene="pflA"
                     /locus_tag="IW656_001346"
     CDS             8668..9429
                     /gene="pflA"
                     /locus_tag="IW656_001346"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0837058.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9514..10440
                     /locus_tag="IW656_001347"
     CDS             9514..10440
                     /locus_tag="IW656_001347"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0837059.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10622..11515
                     /locus_tag="IW656_001348"
     CDS             10622..11515
                     /locus_tag="IW656_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0837060.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11649..11774
                     /locus_tag="IW656_001349"
     CDS             11649..11774
                     /locus_tag="IW656_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0837061.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11908..12540)
                     /locus_tag="IW656_001350"
     CDS             complement(11908..12540)
                     /locus_tag="IW656_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837062.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12688..13230)
                     /locus_tag="IW656_001351"
     CDS             complement(12688..13230)
                     /locus_tag="IW656_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0837063.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13365..13754
                     /locus_tag="IW656_001352"
     CDS             13365..13754
                     /locus_tag="IW656_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837064.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13758..14522
                     /locus_tag="IW656_001353"
     CDS             13758..14522
                     /locus_tag="IW656_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0837065.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14651..15598
                     /locus_tag="IW656_001354"
     CDS             14651..15598
                     /locus_tag="IW656_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0837066.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15728..16468
                     /locus_tag="IW656_001355"
     CDS             15728..16468
                     /locus_tag="IW656_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837067.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16759..17427)
                     /locus_tag="IW656_001356"
     CDS             complement(16759..17427)
                     /locus_tag="IW656_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0837068.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17561..17848
                     /locus_tag="IW656_001357"
     CDS             17561..17848
                     /locus_tag="IW656_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837069.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18264..19802
                     /locus_tag="IW656_001358"
     CDS             18264..19802
                     /locus_tag="IW656_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0837070.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19799..20764
                     /locus_tag="IW656_001359"
     CDS             19799..20764
                     /locus_tag="IW656_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837071.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20751..22073
                     /locus_tag="IW656_001360"
     CDS             20751..22073
                     /locus_tag="IW656_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0837072.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22136..22240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22314..23057
                     /locus_tag="IW656_001361"
     CDS             22314..23057
                     /locus_tag="IW656_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837073.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23072..24064
                     /locus_tag="IW656_001362"
     CDS             23072..24064
                     /locus_tag="IW656_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0837074.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24076..24813
                     /locus_tag="IW656_001363"
     CDS             24076..24813
                     /locus_tag="IW656_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837075.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24803..25525
                     /locus_tag="IW656_001364"
     CDS             24803..25525
                     /locus_tag="IW656_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0837076.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         8865 a         4337 c         5325 g         7072 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat aaatccttat
     6001 acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg tcaacgttga
     6061 tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa aacccttgca
     6121 aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac agaaatttag
     6181 gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa agaaggcgta
     6241 gacactagag actttattca aaataactac actgaatatc gcggtgatga cagctttttg
     6301 gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc
     6361 caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac tattacttca
     6421 cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca gacagacaaa
     6481 cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa
     6541 agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg gcgcaaaaca
     6601 cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa
     6661 atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt
     6721 gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca tgatatgact
     6781 ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac tgaacaatat
     6841 cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat ttctaaacca
     6901 gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc agcaatcaaa
     6961 tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga tatctatatc
     7021 caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt gatcgaccac
     7081 ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa ccaattattc
     7141 tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga tggtcgttca
     7201 ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg accttcacca
     7261 gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag aacatatgct
     7321 agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca gcttcgtgaa
     7381 caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa
     7441 gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata tgccatcaat
     7501 ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc aatcacatca
     7561 gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga ttggttagca
     7621 gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata ttcttatgaa
     7681 gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac aggtattgct
     7741 gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc
     7801 cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc taaatacgga
     7861 aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt catgggacaa
     7921 atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt gacgattact
     7981 tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga agctggaaaa
     8041 ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt attagctagc
     8101 ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc aaatacccaa
     8161 acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat
     8221 gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt attcagtaat
     8281 gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac gatccgggta
     8341 tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga tgtcttgatg
     8401 agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga
     8461 aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa
     8521 cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca taacgaagcg
     8581 gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg tacttaaaat
     8641 gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta tgttcattct
     8701 atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt tatgcaagga
     8761 tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg cggagggaaa
     8821 gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta ctggggagat
     8881 aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt tttgatcgaa
     8941 ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg cggaaaacct
     9001 tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta tacagattta
     9061 ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac gcattgggac
     9121 aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt
     9181 cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg actagacaac
     9241 tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca tacgatggga
     9301 aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga accacctaca
     9361 aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac aaaatattta
     9421 acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt tttgctatta
     9481 ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt ttttggtcac
     9541 caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt gcaaaatgaa
     9601 ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac acaatacgca
     9661 ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc agaaaatgca
     9721 gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat cagcgaagta
     9781 aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc taatccattg
     9841 tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact atataaagaa
     9901 aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat catttcagat
     9961 acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc aaaagaactt
    10021 gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa agcaggaaca
    10081 aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag tttcccaatg
    10141 ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga agtgatggat
    10201 cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg ctacgatctg
    10261 ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt tgctggtaaa
    10321 ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg tgctttgctt
    10381 aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt atttagctaa
    10441 tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata aaaagaaaca
    10501 tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg actcttgttc
    10561 ttcctccttt ttacgtaaaa gccttttaag taaaataaaa cggatagaaa agagaacata
    10621 tatgaactat tattcaaaaa ataaggaaaa cttttatcaa aaactgaccg atgatccgct
    10681 tttttcttca ttgaccgatt atctatatga acatcgagaa caagaaacga ttctaagaga
    10741 attgaaaaaa gaattttcgc aaaataaatt ttcccatttt cttgatttat tgatcgatgc
    10801 aggattaatc aagagggaag aacgaagata tcatttgaac tttccaatat ttgatcctaa
    10861 agattacttg caacaagcca cttcggctgc tgaaaccata gctgaacaat tgaagagact
    10921 ctctgttgat gaacaaaaat tagcgatggg agaagttatt tgggcgtatt gtttcgaaga
    10981 tgaacggaaa gaagcgtatt tttatggagt aagaaacagc cgagagacag aattgctgag
    11041 gacaactgca ggaaatgaga agtaccgttt tatcacactt tcctctatag aacacttccc
    11101 gcttactttg gctaattact tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt
    11161 caaagaatta gccgaactta ttggagacgt aaatgaatcg tacttttttg atcaaataga
    11221 agttatcgtt gacaggatac gtaagaataa atataaaaat cgccggccta gtattttcca
    11281 tcagtctcta cttgtgacga atactataaa ggaagaagaa tcattcacat tagaattacc
    11341 tattgtcgaa aaaaacaatt ttgagattga attaccaaca ttggatcctt cattgacaat
    11401 ggaggaaatg gcctttctta aacgacaaat cttttctgag ctgagcaaga aatttatacc
    11461 gcatgcattt tcttatatca aagaatatgg aacagtttta gtgagtaaaa cctaagagag
    11521 cagtaagcaa aaattatact gactcttgca ggaggagcct ttctcttcct ttatggttaa
    11581 aagaattatt tgagtatttt gcctatctac cacctattag attcactaca tggtctggaa
    11641 acaaaggaat gcaaaaaaat ggtgagaaat gtggaatgac aaaggaagta gtgataagaa
    11701 aagttagatt tttaaataat caatattatg attcagtcaa gtatggcatt ttatgggaag
    11761 aactagctga ttagattagg aagtccattt gataatccag aatcactaag aaacgaatgg
    11821 cgagtcatta ataatttatc caatcttggg ttattctttt agattgaagt gtttaaaact
    11881 aaaaaagcat ttatgaagtc gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt
    11941 ttttctttgc cacataaata cacctagact gacgatgaag agcatatcca acagatttcc
    12001 tgcgacttgg atcgtcgaag ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa
    12061 caagctgtaa ataaaacagg tgacaggaac taatcgaaag aagagcggac atttcaattg
    12121 gctaaaaatg agtgaaaaaa taaaataagt aaaaatggat aaaatcacga ctaaaccatt
    12181 catgataaag aagaaaagcg tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga
    12241 aaacgtttta aatgcaatca atacgcttga agcttcgttt aacgtaccag gatgtcgatg
    12301 aaaaaaatag gcaccgaaat aaacagaaaa cagtaagccg caaagagaag acaaaatcaa
    12361 aaaaaacgga ttttcttcgg cttctgtgat atcagaatga tccaaaagca accagcctaa
    12421 tgcactagaa ggcgcatgtg aggtcttaag gtatgaataa catagaaaaa ataagatcgt
    12481 caaagttgca aaaaaagagc ctaaatgtaa aaaattagtg gttctacgtt gaatttccat
    12541 aatatctcca aattaaattg ataagtatat aaagtataat aacaaattga tgagtatatg
    12601 tcgatgtttt ttaatcaaaa taataaataa aaactgtcta acatttaaaa tttaatttag
    12661 atattagaca gtttttaagt ttgtgagtta ttttccttgt cgttttctca cttttttagt
    12721 ccagaaacca cctgaaatat attttggctc ataagaaatg ataaaagcac gctcttcgat
    12781 ttctttgatc aggcgataca gttcacgttc gctttttcta ggagaaagta tttcgagaac
    12841 tagccgctca ccttccagcc ccattgcaga agtctgtgtc acaccataac cgtgttctct
    12901 cagtataccc ggcaaacttt tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat
    12961 gatgtatcct aatgccaatt tgtcttcaat caaaattccg acactgatac ctactgcgta
    13021 acctaacgca tagacaaata aattcagtgg attatctaaa cgatctaaaa caagggataa
    13081 gccgacgata taaatcgtga tctcgaccat actgacaagt ggagcaatca gtcgatagcc
    13141 tttcattgtc aacatgaaac gtaacgtgtt cagagtgata taagcaaaat taatgaggaa
    13201 gatcatcccc aacatttttg gatcaatcat ttgtaaaact cctttcattt atataacaac
    13261 aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt tatacataca aaaaaatgat
    13321 aaaatgatta taatagaatt ataaagatag gttaggtgat taaaatgaaa acggtcaatc
    13381 gtgtgttttg tgccagccac gtgctaagtc tccttctcag tatatactta ggtgtctatt
    13441 cgattcaaca gccaattgag gtagagaaca ttgtttttca gatgtcgctg acagatggtt
    13501 taattttaag cgtttttttc tttttgatac tgtttattgg taatattttc ggtagcgttg
    13561 ccagtttttt gaatttttct atttatccac tcctttcgct agcattagga gtggtgggac
    13621 tttgttctct gtgcttattt cccgagccat tcagtcctgc atttatatta tttggtacac
    13681 tgaacctgtt ccagacaact gttggagcat ggttgctatg gcgatcagga aatttaatga
    13741 aaataggtga gtaacaattg aatccaatta tattgaaacg ttctgtccat catattccag
    13801 tgttagaagt tgttctgaaa gaattaagag aggaacgtat tcccgttgtc atctattatc
    13861 acggatggca gacaagtaag gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg
    13921 gcattcgtgt gatcctgcca gatgcgatga atcatgggga gcgcaaacag ccggtttctt
    13981 ctattccatc ctatacattt tgggatagta tctacggaaa tctgtttgaa tttgacacac
    14041 ttatcaagca tttagaaaaa agaggtcttc tacatgatca gcttgcagtt ggcggggttt
    14101 cgatgggggg aatgacaaca tgcgccttgt tggctaaaca tcctcatata gtagcgggcg
    14161 tgtgtttgat gggttcccct gcacctttgg aatatggaaa agaaatcgta aggcgcgcat
    14221 cggaatataa ttatgtcgta ccaaaagact attttgattt gatttcatgg gttaagagct
    14281 atgatttgtc attgcagccg gaaaagttag ctggacgtcc tctgttcttt tggcatggac
    14341 aagctgacga aaaaatccct tatatacaaa cggaacgatt tgtagaaaaa aatcggcata
    14401 agtcttacgg aaaagaaatt gtttttcgtt cctcaaaaac acagcgacat atggtacaaa
    14461 taccactaat ggaagaagct tccacttttt tagcaaaaaa tttgataaaa aagtataaat
    14521 aattgaaacg gaaaatcact catgacgata gaggattttc cgttttttat tttgggctat
    14581 aataaactct gttaggaaaa tttggtataa ttgatagtaa gaatgacaac atgttagaaa
    14641 ggaagagagc atgcccactt tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac
    14701 ggtctctcga gtcatcaatc atccggaaca ggtgactgac gaattgaaag agttagtttt
    14761 ctcagcaatg gctgaactgg attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa
    14821 tcggtcgcag atcatcaaat tatttatact ggaagaaatc gacacaactg agccctatta
    14881 catgaatttg ctgatgggaa tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt
    14941 agttacaaat gatgccttcg acgtcggttc ttgtgatgga tacatcatca caggtgtcag
    15001 aaaaaaagac ttcgattgga tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa
    15061 ttctgaagga tatgattatg tagacagcga taatgaatat ggtacagcaa aagcaacgca
    15121 atatgcctta gaaagagatt atcaaacgat tatttatatc ggtatcgaag tggacgagcc
    15181 ttttgaaatt tcaagggaaa ctggttattt atctgtgatg aaggaagttg atcttcctag
    15241 taaaatcttg cggtttccaa atcattcgac agaagtagaa agatatatga cagaaaactg
    15301 ggcagatttt acagataaaa cttgttttgt atgtagttca gatcggctag cactaggagc
    15361 agtaagaggg attataaatg ctggaggaca cttgcccgaa gaatttggtg tgattggatt
    15421 tgatggggta tttttagacc aaatcagttc acctaaattg acaacgatca agcaagatat
    15481 tatacgtatg ggtgaagtat gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc
    15541 gagtcaaggt tatcgtcgtt tttttcctga actagttatc agagagacaa cgaattaagt
    15601 cttattacta tatatatatt tatgaaattc atcgctattt tcttaaaata atcagcgcat
    15661 tgctactgaa aacaatgaat ctgtgctatt attgagaaaa gttccatttt tgagaaggat
    15721 ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt
    15781 aggcgtaaaa ttaaccaacg acaagaagcg agtattattt gacggacaag aagatctttt
    15841 gactgaacag aatcagtccc cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa
    15901 caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa
    15961 ggaaaatttg gaaaagcata aaggaaaatt accggattat accggccatt accaatcaga
    16021 aatcacatcg acaggacgaa aaaaactttt tggtgattcc tctcgttctg tcagacaaag
    16081 tgaaacaagg acagagtcca agcatactac acgaaacgca tctgttgaca cgaaggtgcg
    16141 ggagcgttct tattttgttc ctaaatatgt acccgcatcg attatcccag accctagaga
    16201 aagcaaaatt tctgaggatc atttaatcga atcgatgaaa aaaacggatg actcttatct
    16261 gttgttcgat acagaaccga cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg
    16321 aacattcaat cgaacaaaaa cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa
    16381 aaaaagccat ggtatcttag aacgctcatt acaaggattg atcgatgatc aaggaaactc
    16441 tttgggagaa aatagctatt ttaaataagt gatatacaaa tttatattcg gctacagttg
    16501 gaaaggaaac gctgcggcgt ttccttattt tatactctat ccgaaagttt atatcgtatc
    16561 ctcaaatttt tttgagttta agcgaatttt ttctcccaat aagtaagaaa ataatggcaa
    16621 aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa tagtttattt ttttataaag
    16681 aagattttac tgaataatat cattttagtt tacataatga tatttctgta aattaaataa
    16741 agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt
    16801 ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac
    16861 tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag
    16921 atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc
    16981 aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct
    17041 tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact
    17101 attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa
    17161 gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa
    17221 caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc
    17281 aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg
    17341 aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa
    17401 ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca
    17461 acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt
    17521 ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt
    17581 gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga
    17641 acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt
    17701 atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa
    17761 ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa
    17821 agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt
    17881 caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta taatattcgt
    17941 tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg ctgtttcttt
    18001 ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt gacaatttcg
    18061 tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga tccgactttg
    18121 ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt ttttgttcgt
    18181 gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt ttatacttaa
    18241 agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt gaaagaaaag
    18301 gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct ttgctgattg
    18361 cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa atggcagaag
    18421 tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta ttaggtttac
    18481 ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt atttacgctt
    18541 tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg tctattgaag
    18601 aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta gagcttccca
    18661 tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc atcggtacgg
    18721 ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg ttaggtattg
    18781 accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg cttgcaatta
    18841 tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc atcattactt
    18901 tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc ttgtttaaaa
    18961 atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg atccaaatgt
    19021 ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg aattttggca
    19081 agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat cctgagttca
    19141 caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt gctgatttga
    19201 caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc gatatgacat
    19261 tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga tcttttgcga
    19321 acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag atcaaagcgg
    19381 gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa gaagcatatg
    19441 gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa gcaatcaata
    19501 atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag gagtacgatc
    19561 tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca ccgctgatga
    19621 aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa ttaagcggaa
    19681 aaataacaga ggatcaaatg attgatatga attaccaagt caatgtcgaa aagaaaaagc
    19741 cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt
    19801 gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt atgtcatcga
    19861 tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc cttccggtag
    19921 tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag caggagacat
    19981 ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt ggaatatggg
    20041 atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa atattgaagt
    20101 catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag atgaacttct
    20161 tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag agttgtctgg
    20221 aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag atgtgatttt
    20281 gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac aagaattggt
    20341 tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca atatggaaga
    20401 agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta tccaatgtga
    20461 cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt tctttgatga
    20521 accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat atttcttaaa
    20581 tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga aagaagtata
    20641 ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa gtattgggtc
    20701 gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa atgaaaacat
    20761 atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat ggattgaaag
    20821 aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact tgtcctaacc
    20881 gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac
    20941 aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa cttcaaaaat
    21001 tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta gcagaagctg
    21061 aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt gaagtcaacg
    21121 aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct acgatcttac
    21181 cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg cctgtactgc
    21241 ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct acgattgcca
    21301 atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta aaagaatttg
    21361 aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt cagtttgctt
    21421 ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta gtcggaaaag
    21481 agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg aatacagaat
    21541 ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca gtcaatgatt
    21601 atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca aggaaaaccc
    21661 caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg ataacggatg
    21721 caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc agtcctaaac
    21781 ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg
    21841 atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag gctgaattga
    21901 cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat
    21961 tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag ttggatagct
    22021 atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa taatcagcat
    22081 gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa atgaatacct
    22141 gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc cattgaacct
    22201 gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt tactatgtcg
    22261 tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac aaaatgaaga
    22321 aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag attgttgtca
    22381 tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt tctgcattag
    22441 cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg
    22501 gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat tattggaagt
    22561 ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca atcatggtgt
    22621 tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg atccttgttg
    22681 tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg aaagtatcaa
    22741 aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt tatcgaatat
    22801 ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct gctacttatt
    22861 gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta gggtattaca
    22921 tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc atttgtgtca
    22981 ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg tattatcgta
    23041 tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt tattaccgct
    23101 actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac aagaaataaa
    23161 ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc atactggcat
    23221 ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc aaatcatcga
    23281 accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac aatttggtgt
    23341 aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac cagtcaaagc
    23401 aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag acagtaacat
    23461 ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga
    23521 agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac tgacaatggt
    23581 aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc aatggtactt
    23641 tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact atatcccatt
    23701 gaaagaatta gatgaacggt tagattacta tactccggta attattacga atgatcaact
    23761 gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa aaggctatca
    23821 ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg caaaagaaaa
    23881 tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata cggatgatcc
    23941 tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt ttatgcagga
    24001 aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc agtttataaa
    24061 ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat tgaaaacctg
    24121 aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc gattgtcgcg
    24181 ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca gcctatgact
    24241 tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc atttagttat
    24301 atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt tacgttatat
    24361 caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct ggatatcttt
    24421 gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat gaagcagcga
    24481 acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga tgaaccattt
    24541 ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa gctgcctaaa
    24601 aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat ttatttatca
    24661 gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct
    24721 gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca aacgatttat
    24781 cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag atgggagtca
    24841 gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt gtgattcgcc
    24901 agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct taagttacgg
    24961 tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt ttttgaaaaa
    25021 agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac cgatgactac
    25081 acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt
    25141 actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca
    25201 ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg
    25261 ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc
    25321 agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt
    25381 aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat
    25441 cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga
    25501 acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt
    25561 tttggctttc tttgtataaa agtaaactgt ttgattaat
//
LOCUS       DACEJZ010000036        25532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEJZ010000036 DACEJZ010000000
VERSION     DACEJZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.036"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(166..651)
                     /locus_tag="IW656_001365"
     CDS             complement(166..651)
                     /locus_tag="IW656_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAQ0837077.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(653..2089)
                     /locus_tag="IW656_001366"
     CDS             complement(653..2089)
                     /locus_tag="IW656_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837078.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(2122..2973)
                     /locus_tag="IW656_001367"
     CDS             complement(2122..2973)
                     /locus_tag="IW656_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837079.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(3129..3374)
                     /locus_tag="IW656_001368"
     CDS             complement(3129..3374)
                     /locus_tag="IW656_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837080.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            3479..4252
                     /locus_tag="IW656_001369"
     CDS             3479..4252
                     /locus_tag="IW656_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837081.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(4305..4619)
                     /locus_tag="IW656_001370"
     CDS             complement(4305..4619)
                     /locus_tag="IW656_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837082.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6138..6356)
                     /locus_tag="IW656_001371"
     CDS             complement(6138..6356)
                     /locus_tag="IW656_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837083.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(6371..8998)
                     /locus_tag="IW656_001372"
     CDS             complement(6371..8998)
                     /locus_tag="IW656_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837084.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(8985..9203)
                     /locus_tag="IW656_001373"
     CDS             complement(8985..9203)
                     /locus_tag="IW656_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837085.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(9200..10075)
                     /locus_tag="IW656_001374"
     CDS             complement(9200..10075)
                     /locus_tag="IW656_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837086.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(10340..10639)
                     /locus_tag="IW656_001375"
     CDS             complement(10340..10639)
                     /locus_tag="IW656_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837087.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            10841..11638
                     /locus_tag="IW656_001376"
     CDS             10841..11638
                     /locus_tag="IW656_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837088.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            11689..12831
                     /locus_tag="IW656_001377"
     CDS             11689..12831
                     /locus_tag="IW656_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0837089.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(12929..14320)
                     /gene="sufB"
                     /locus_tag="IW656_001378"
     CDS             complement(12929..14320)
                     /gene="sufB"
                     /locus_tag="IW656_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0837090.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14325..14795)
                     /locus_tag="IW656_001379"
     CDS             complement(14325..14795)
                     /locus_tag="IW656_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0837091.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14782..16017)
                     /locus_tag="IW656_001380"
     CDS             complement(14782..16017)
                     /locus_tag="IW656_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0837092.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16014..17300)
                     /gene="sufD"
                     /locus_tag="IW656_001381"
     CDS             complement(16014..17300)
                     /gene="sufD"
                     /locus_tag="IW656_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0837093.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17313..18086)
                     /gene="sufC"
                     /locus_tag="IW656_001382"
     CDS             complement(17313..18086)
                     /gene="sufC"
                     /locus_tag="IW656_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0837094.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18283..19131)
                     /locus_tag="IW656_001383"
     CDS             complement(18283..19131)
                     /locus_tag="IW656_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0837095.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19155..19841)
                     /locus_tag="IW656_001384"
     CDS             complement(19155..19841)
                     /locus_tag="IW656_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837096.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19834..20868)
                     /locus_tag="IW656_001385"
     CDS             complement(19834..20868)
                     /locus_tag="IW656_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0837097.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(21263..21601)
                     /locus_tag="IW656_001386"
     CDS             complement(21263..21601)
                     /locus_tag="IW656_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0837098.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21603..21959)
                     /locus_tag="IW656_001387"
     CDS             complement(21603..21959)
                     /locus_tag="IW656_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0837099.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21965..23152)
                     /locus_tag="IW656_001388"
     CDS             complement(21965..23152)
                     /locus_tag="IW656_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0837100.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23343..24188)
                     /locus_tag="IW656_001389"
     CDS             complement(23343..24188)
                     /locus_tag="IW656_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0837101.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24422..25387
                     /locus_tag="IW656_001390"
     CDS             24422..25387
                     /locus_tag="IW656_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0837102.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7429 a         5139 c         4037 g         8927 t
ORIGIN      
        1 ggattaaaca ttttttatca tagagtttta cttatctata gaattatgtc tgttcctaat
       61 tcagtttgat gaattaactg ataaaaaaat atctactatt taaagataca gggataagta
      121 agtaaaaaaa taacatgtca ttagatagta gacagttcca actctttatt tacctttttt
      181 gtatgctgca ctgtcaatcc aagttcctaa attgttgtat ccatcatcac ttatataatc
      241 atattggcca tcaataattt cgtcaaacca caaatattct ccatctggtt tttgcccaat
      301 tattttaatt actgattgac tagtagttgt catctggtta aaatctttta ttttacctat
      361 attaacggcc aaaatagcat ttcctcgctt ataactctcc tggatttcat atttaacata
      421 ttctcgatca agagtttctt tacctatcag caccacagtc actgatgttc caattaattg
      481 attatctatc cagtttttta tagcaatatc tccattacgt ttaactttct caaattcagc
      541 cttatctaca aaaccagctt cctttgttcc tcgcaaagtc cagctgtttc taactaccat
      601 tgctcttgtt acatctgcat aatggaaact aaaaaaaacc cttctcacca tattaatcct
      661 cctgaatact atttattaaa ctgacaattt gttcaacttt tttatctact tctccttcag
      721 tatctaattt ttctagcgct tgaaatttac tttttaagga atcatcaaaa taatcactga
      781 aattctcttg aatcttatca aacaaaacct tacttgcacc tttggtaaat ccaatcggaa
      841 ctactatatt ttcattttga acagataatt caaattcttc aatcattcca tctgctgtaa
      901 ctatttcacc tgattcaagt ttgtttccaa aaacaaaaat actaatccca gactctgcaa
      961 taatgttttc tcgatatctt ctccactcat gaggcatatc aaggggaagt ggtcttaaag
     1021 aaagtacgtc ttgtatgtta ttttttctta aatttcttct ctgctttaga actcctgaaa
     1081 caatactatt accaattcca agtccgtaac ctgatactat tctataaccg gattcagaaa
     1141 gcctatatgc taatttctcg acaaatttta atcctttcga ttcagatgta aatggagtat
     1201 aatcaacagc acttcccgaa ataaaaatag tttttctcaa atactttttt ctaatacttt
     1261 tcaaaatatc agtaatctca gaataatcat ctacttcaac aacgtttatc gaaaatctgg
     1321 acaaatcatt gatttgtagt cttctttttg tcagttcata attatatctc tcttgatcaa
     1381 aaaaatcctc agaatcgaca aaattttcct ttaatatttt tctcattata caataatgag
     1441 gtctttggtt ttctctcaaa acccatctta tatctgataa aattctttct aaattagggt
     1501 ctgtaaaact aaatcctaaa aagagaaaag tttttgttaa caaatctcct tttaaagttt
     1561 ctttaaataa cgcgtacgaa tcatcatcat atttttcata atcatctctt gttattactg
     1621 tcgaactgac atcatctata tcaccatgca ttttataaac aattgtatcc acatttggat
     1681 tagttgttgc taactgagta taatttttct taacctgagg gtttttataa gcttctcgga
     1741 ttgatttttc tattagatga tcataattag ttgtccaata agtttcaata ggtaaacttg
     1801 ctagtattcg atgattttca gatatttcta attcttcacc aaaattatcc ataagtatct
     1861 gattcaaaat tcctcttgat ccaccatttt catttttata atattgagcc agagtaacta
     1921 agtctgtttc ttttttagaa tccaacccga tttctgttgc aggatcatta agtagttccg
     1981 gccaacttac atatccttgt gacatcgata atcctgcacc tgcaaatatt gctgcatttc
     2041 ctttttcaat agcttcagag aacctcttta ttaaaacttt cttatccatt ctacccggtt
     2101 cctttcgatt cagtaaattg attagagatt agtgaaaatg gtgtttttat ataaaatatc
     2161 taatgtatta cttcctttag tgcattccac agcatctaaa gcccattttt tcaaatcagc
     2221 ttttcgtaaa taagttaatt cacttaaatt taaagaatat tttatttgca gattttcttg
     2281 cgaagaatag tgctttataa tgcttctaac atcctcggtg ctcatttgag gattactttc
     2341 caatcgtgga ttcttttttt ccactattga agctgtcgtc agttcgctat atagccatgg
     2401 cgaatacgta aatttctgtt cattagtgat ttcattgctc aaactaattg aattaggtgt
     2461 attaagaaaa aacagacatt ctgtactatc aatcatatta tttaaagctg tagaaagcat
     2521 catatgaaca tgactagttg ttacatttct tttatcataa tcatatgttt ttgtttgttc
     2581 cttatagcaa tatttttggt ctattttttt taataaatca gaactgtatc cccaaaccga
     2641 agaatcaata aatgcagtaa gaccaaaatt ttcaaatagc catgatgcaa aacgtatagc
     2701 taatttttca tcagcatgag agtgagatat aaaaatatct gcttttatag ttggaaacca
     2761 atctttaata atttcgtctg catctagaat tttgctgtcc ttagaataat aatcaactaa
     2821 atcattttca ctttcaaata gattttctat tctcttgtta atagattttt ggttgtgatc
     2881 tatttgattt actacatgtg tatatgattg cgagcttgag gagaatgcat ttaagtcagc
     2941 atttgtaaaa tccaattcat atgccttata caaggatatc cctccattct tgtattttga
     3001 tccacaattt attaggcaac ataacagtat accctatcgt tacatatccc attcattatt
     3061 ataccagtat ttcttcgtga cacataaggg tgtaacataa taatttcaaa cgaaatgact
     3121 acaagtagtc attgaagcaa atcttcataa gacacctgta gaatatcttt gatggctttc
     3181 aattgatcca acttaatgtg ttggcggcca ccttcaatct taaccaacgt ttccctagtt
     3241 atagcaattt tacgaagctg taggtctcta atcagctcgg tttgaccaat tcctttttct
     3301 aatcggactc gtctaatatt gctaccgatt acattttctt tatcaataat tttttcctct
     3361 ttcaagaata gcacaccttt tggactaaaa ttagtccttt ttcttattaa tcttaaagat
     3421 tcggtatgct acaatgggac tagtattagt ccatattcaa ttttaaggag gaattttttt
     3481 gaagaaattt gcaatcattc taacttgcgc tttactcatc ggaactataa gcgggtgtac
     3541 cacttctgac acaagttcta caagtacgga aactagtaca acaacctcag aaaagaatta
     3601 cccaatacat ttttttccgg ttccagatgg atttgaagct tattatcatg ataaaaaact
     3661 aaatgttcct ttatcagaaa ctgaatctac tgaaaagatt ttgccaattg aaattgaaag
     3721 aaatcaagac gatataaaac taaaagctga tcttccctta ctgaacagca cacaaattga
     3781 tagacatgaa aatattaaag ttaatattga aaaaggagcg ttagcacaac tttatactga
     3841 aaaagttgaa caattttttg aggctggtgc caataaagac ttttcaaaaa taagcgatta
     3901 ttcggataac ttttttaatg agacccaaga tgaaatcacg aactcaactt ttatggctga
     3961 agatatgtac aatatgaagt tgaaaaaaat aaagtcgtat gattatccat taaatctaac
     4021 attaaataaa gggatactct cttttgtatt agcaggttac gttgactatg aatatacagc
     4081 agattatttg gagacgcccc ttcaacacga taataacgat attaatttta gatttatcta
     4141 caattcaaaa agtaaaacat ggttagttga tcaattgagc actataaata cgggatttca
     4201 tgaattacct aagcctgaca atgaaactgc tttggtcatt aaaacattct aaaaaagaaa
     4261 cgttgaagga ataattattc tttcaacgtt tcttttattg tttttcagtt tacggtctta
     4321 cctgagcgaa taatctgatt tctaaatgat tggaaaactt cttgtggttg ataataatct
     4381 gtatctattt caaatgtttc tgagaataat tcattgaatt tttctactga aatgatgatt
     4441 ttaccattct cctctcgact gttttctatt atttctgcct ctaattcaaa taaatctagt
     4501 gacgaactaa aatcataaat cctgtcatat tgaacaaaaa aatcaagaaa aagttgctca
     4561 aactcttcct tatttatcac tagtgtttca agcggaacaa ctttattttc atcagccatt
     4621 cttccaattt cctcactttc attttcaatt gtccattgtt gcggacgtat cagattttct
     4681 ctatcatttt tcagtgtata gatcacttgt aatacacaac aatgctttgt aaacttattt
     4741 ttttagttag tcgaatcttt ttaaatgtgc gtttcatacc atgcgcttga tatattttct
     4801 cctatttttt aaatcgttta tcacaacttt tatcgttttt tcttctgaaa aaaatcttgt
     4861 ttacatcgtt acatagtttc caaaaagata cattactagt agaaacaaca agattaattt
     4921 tacttcccac tgaaatggga agtatatttt aaatattttt tctccttcaa cttacactat
     4981 aaagtatctt aatcacacga tatgctagcc actaaattca tcatatttga ttcctttttt
     5041 aagataatgt agcatctttt tatccaataa cgcaattgtt tttaaggata aatttcttaa
     5101 gatggtacat ttcaaaatgg catttgacat atgaaacaaa aaagaaagaa aagagcataa
     5161 gggaaaactg atagcacctt cctacaggaa ggtggctaag ttggctgtgc caactggttt
     5221 tctttcaaaa tcatttcata atttttgata tcataaaaaa atccattttg gacctatttt
     5281 cggtcataat ttagtaccta cttttggtca tagtttcgtc cgtagttttg gtcatagtga
     5341 ctaaggcttt tctgttcaca aaattggttt aaatttgctt tctttaacac ttcatttata
     5401 tcatagcaat tataatgttt acacaacatc attcctaatt ttaacggttg ttcctcttta
     5461 tattttttaa gttctacatt tttttgcgtt taattacttt tctcttttct ttatattccg
     5521 ctccaacttt ctctttaata gtgataatag aattacatat ttttgttaaa cttccttaaa
     5581 aaaattggat ctcgattact caaatttgct tgcaggcttt tctaattgct ttgttttttc
     5641 ttcctaatta tttgatttta tttccatttt cttctagtga aaatttatta tttttaataa
     5701 tcgtacgcgt agaactgatt tcattttttg tatctgtttg attatctaac gtttgaaatt
     5761 caaagttttg attggcttta tcaatatctt tttatactac tttatttgca agatatattt
     5821 tcttgcttaa tttggtcata gtttcattcg tcacttcggt gatatttttt taatttgcaa
     5881 ataaaaagag ttaaaccgat atacattagg ttcaactctt ttgcaggttt catgggtgta
     5941 ctatcacttt taatctgata gatgtaaacc tctctttatc tttttgttac ttcaattttc
     6001 aacaatagct cctttatagg ttgtcaatat tgctctacgt attctggctt tttaacccat
     6061 tatttctcta aagtggaaga gaataatcgc ttaatcccca ctaattcctt gtcgtaaatt
     6121 catgtcaaat acaattttta aaagccattt aattcatgga ttatctcttc acttttttct
     6181 agataacaat atttcaagtt gttttttatc tcatgtttca tctttaattc ttcttgaatc
     6241 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     6301 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa tttcatgaca
     6361 attccccctt ttattttttt aatagattat cgaaaagcgg attaggatca ttaagtgtat
     6421 ctctgaataa ctcgttgtct tcataggtaa atcttcgtct tggttttgat cccttatcat
     6481 tttcacggat ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg
     6541 actttctgtc ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt
     6601 tttgaacgtt ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta
     6661 cattctctct tacatccatt gatttaaacc cttgactact tgtagaattt tgtacattct
     6721 ctcttacatc cattgattta aacccttgac tacttgtaga attttgtaca ttttctcgaa
     6781 catcaattgt cttagtacct tgaccatttg aagagctttg taaattctct cttacatcaa
     6841 cattccgtcc gacatgacca ttcaatgaac cttgaatatt ttctcggaca tctactgatc
     6901 ggccaccttg actacttgta gaattttgta gattttcttt gaccaatttt tctaatccgg
     6961 tatttgagtt tttagcttct tggattcttt ctttggtcaa cgcttctttt tcgtccgtat
     7021 tagctcctgt acgcccttct acacgtttcc ttaacatttc ctcacgattc attgcttcag
     7081 gcgtgtattt agcttcttgt cgcttctgat taattaattc ttcagcagac ttagcttgac
     7141 cgtgcatagc atttttacgt tcttcaatat cttgttgaac tttttgctga attgcactta
     7201 caggattatt attcgattca gcattatttt tcattgcttc acgcatacgt tcagaactag
     7261 acataattct atcggcattt tcaaccttag caccatcttt tctatccgaa agacgtgttg
     7321 gcatggaatc agcatatttt ccagtttcat cttctttatt tttagctaac ataggtttgc
     7381 tatttttatt aatagcggac actgtaccat catctaaacc ttctttgaac ttattcttgg
     7441 tatcattagc catatcacgc atactcttaa atggttttat tgctctttca ctagccattg
     7501 tagctccatc tgaagctaaa cctgataatc cacgttcatg ggcatatccc aaagctcttc
     7561 cagttttgcc agcttttttt gatagagttt cgccgaaatt cttttctggt tttcgatctt
     7621 ctccttctcc actgtctgat ccatttccat ttccgcttcc tttagcacga ttaaatccag
     7681 ttgatccttt tctaaacagc attgcgccca taccaaaagc agaagcaagt tgcccatagc
     7741 cttctttgag tccaatatca acaccaaagt atctcatgac tgttccactg ccttttatca
     7801 gcacaaacac agcacagata taagcaattg tttttataaa aatatttgtg gagatacctt
     7861 gtccagcatt taaaaaattg ataaacatag caaacataga tagtccaaac ccttgaaatc
     7921 caactgttaa gtaacattgt agaatatctg atagaacaac ttttgaacgt tgtcctgttt
     7981 caatatctgt cgcaaaaaca agaacaccta acacacgttt aaatgcaagt tctaatattg
     8041 ctgtaatgat cacaaacgcg ctgaacacat aggcgattgc caacgctgtc aacccaatcg
     8101 aaatccccca aagatttgct tgatatcgat aataacctga tttcaagcta tcagaaaatg
     8161 ttgataagaa gttatcagaa aactttgtgg caacaaattc attattagaa ttttcaacta
     8221 attcatagcg aagattatcc gcagctggat tttctttttc caatttatca atcactttat
     8281 ccgtcaacac ttgtgttaaa tctgtctgtt gaaaattttt tcgtgttaac ttatttcgat
     8341 cctccgtttt atcaatgctt gagtattgat ctgttttatt aatatatgcc agatctgtta
     8401 ctccttgttt tatcaatgac caagcgactc cgtcatctga atttgtaatc gtagtagcat
     8461 cattgtaaaa tattttggca aattgaatac cgctagaaat cattgttggc attaaaagaa
     8521 ttaacgctac agacatcact atattcattc caacagatct taaatctatt gttccttttc
     8581 cagtaatcat cttgtagcca acaaacatca atgaaaggat cattaatcca acgacaattg
     8641 tatttatgac tgattgataa acattattta gccctgtact ttcaatgaaa ttgaacaaat
     8701 ccaacacatc tggaatcata ttttgaatcg aatcagtcac agtaaacatg ccttttacca
     8761 cagcccatcc aagccaaatc ccgaactgac taaggaaatc tccattgtat ttttttaggt
     8821 aaggtctata aagagaatta ttcaaaaact ctgtttgact ttttgtgtca tcaaaccaat
     8881 tgaaaatgct agcttcaaca atattacttg taaaaacaac gctaatcatt aaaacaagta
     8941 cactgagtgc taaccagttt atttttctac taacgatttt ttctttaatt gctttcatag
     9001 ttttctcctt cagcccattt tcttaggctc tcagagagct gctgagccat tttttgatta
     9061 attatgatat gagttacaca attgtcattt tcagctctta aatcgagaac tactgtgttg
     9121 aagcagatac tctcgattaa ttcgagagta tctgtatttt gatcaagaca ccacatttga
     9181 tctattaagc gatttttaat catgattctt ctcctttcaa ccaattatgc atagtaattt
     9241 caggtaactc tttgatttcg aaagcttttt tgaactgatc tataggctct acattttcta
     9301 aaaattgact tttatatctt ttctgaagtc cattcaaaaa atatgttaca taatcgtata
     9361 acccttcttc agtttttctt tgaaaaaagc atgcttcatg ttgttttata atacactcag
     9421 caataagata ctgagtattt tcagataaat attcaatgat ttttctgata tcttctgtat
     9481 ctacattagc gtttctcaaa tagtgataca cttgaaataa ttttcgacca tcttcgttat
     9541 ttgcgtcata aagaacacta atcttactgt aaatcctctt tatattaata tcctcttctt
     9601 ccataacgag agtagtcttt gatatgttct gtgaagtagt ctttgtatac gtatatacaa
     9661 atttgtacat acctctacct ttttttaaag agacatatct ctttttttcg atacccctct
     9721 gacgaatttt cggcatatac tccttttttt cgcaatccaa agtaggaaaa gtaatttcta
     9781 tcaatttatg tacatttaaa acaatgtaca taacatttga taatgtaact gctcttttct
     9841 cataataaat atttctaaaa acccttttga taatcccctt tttctctaag tacacaattg
     9901 catcttttat agctcgttct gataatcctg tttttttgct taattgctta tatgattttt
     9961 gcaaaatatc ttcaagaaac tttttttgat aaccaagtag ctgaccatca tcgttaaaaa
    10021 tttttttagg tctataccaa taggtaattt ctgctaagat aactattgca ttcaaatagg
    10081 gcttctcaac accttttttc gttttttttc ttaattgatc gtaccatgtg tgtggattac
    10141 atttccgtca aaatttattt ctctaagttt gtcaactaca acatttccag aagaaaacac
    10201 cttttcacct tcttttttga tataatagct ataaataaag ttttctcagc actttattat
    10261 tgagtgttcc cccagtctgc aaacttttgg gaacactttt tttctttttt taaagcattt
    10321 ttttataatt tctacttcat catcttccaa tattttcccc tcttttcatt cgagcaaaca
    10381 taaactcatt aactgccttt ttcgaataaa attttcttgc ccctaattca actacgggca
    10441 atccctcatt tctcacagca ttttttaaaa agttttcact cacatttaag tattccgcca
    10501 tttctttttg attgagatat tcccttgtca tccccgcatt ttcttgagcc ttttcaatag
    10561 cttttgtagc aatttgataa aactgcttta gaaacatttc ctcctgttct tctgaaatca
    10621 ttaattttat tgcttccaca aaaacctctc ctttttaatt aattattcta taactcctct
    10681 acctttataa gtacaaataa atacaaattg cgtaaaaaaa tgtatttatt tgtatttata
    10741 agaataaagt atcatactcc agataaaaag taaatacttt taaatacttt tattttataa
    10801 atatgatata ctatctttaa aaataaaaag gagaaaaaag atgaagccaa atccttcaga
    10861 agcaggaaaa cgaatcaaac aattacgcct ttcatgtggt tttactatgg aagaacttgg
    10921 aaggaaaata gataattcac cacgtgcgac catatccaac tgggaacgtg ggaccaattt
    10981 accaaatcca caaaagctga aattattatc aactatcact aattcaacaa ttgactggat
    11041 taaatgggga actttagaag aatatataac ttcatacctt atcgatatag ggtatgagct
    11101 atatattaag gacttcccag aaatacctca caaagtcttt aaagacattc aagaaagata
    11161 tagtgatacg ttttcgctag ataaagacta tgagttattg aatcctataa taaaaaatat
    11221 atttacaaaa tattactcaa aagattttga aatttatcta aatgagatac ttgctaataa
    11281 tatcaatcct ctgattgaag agtacgctaa ccaaagacac tctatctcta aagagctatt
    11341 tttcaaaaga gccaacagta aacttcaaga caagataaga aatggtgatt tcaaatacgg
    11401 tgaaaaagaa agaatgattg attctttaaa acagctatta gacgaaatgg acttagccta
    11461 taaagatatt tcaaagtatt catctgtcga ggatttcttt tataaaatta ctaaaaacca
    11521 atttgaaaca gaaaaattta tttcagatct tagtaaaaaa tacaatttcc catatgagaa
    11581 aaatagtata atcgcaaaat ttttaactga taatcacgat aaatttaaaa ataattgatt
    11641 cttcaaataa cttctctacc tgcaataatg aagtaaggag agaatcaaat ggcaacattt
    11701 tttagttata aattacaaaa tggcgaaaaa tattggggat ttgaaactta tttagggaaa
    11761 gatcccaaga ctggaaaaga tatacgtaca aagcgtagaa gattcaaatc aaaagctgag
    11821 gcgcaaacag cacttaatag actacttgta gactttaaag aaaaacaaga gtttaaaatc
    11881 aatgttacaa cttttcaaga gttgtatgaa ttatggattg ataactacag aacaagagta
    11941 aaaccgtctt ctgttgccgt tgctcaacgt ttttgcaaaa accatatatt acctgctttt
    12001 ggggaattac gattggataa aataagtgtt agctattgcc aaaaacaggt gaatgaatgg
    12061 cacaaaacat taaaacaata tggtttcttg agaaaaacaa ctgctcaaat atttaaattt
    12121 ggagtagcaa tggaagtctg taaggataat cctatgtcta aaacattgct tccaagaaaa
    12181 attgaagaag aacaaccctt gaagttttac acgaaagatc aattacaact gtttttacag
    12241 aatactaaag aaaataattc agttaaactg tacacttttt tccgtttatt agcttataca
    12301 ggaatgcgta aaagcgaagc attggcgctt caatgggaag atatagacta tttcaataaa
    12361 acaataacaa ttggtaaaac tatcgctcaa gacgagttta atcaagtagt tctacaagta
    12421 cctaaaacta aaaattcatc aagaacgatt cagcttgatg attttacact taaacaactg
    12481 cgtatttggc aacaagaaca aatgaaaata atgatcttat atggctataa cacgaattca
    12541 ccaaaacagt ttcttttcac tacaaacaca aacaagttgt actaccccca agtagtaaac
    12601 gattggttag actggattta caaaaagaca ccgatggagc ctcaaattac tccacatgga
    12661 tttaggcata cccactgtag cttattgttt gaaagtggtg cgtcgataaa agaagtacag
    12721 gaacgattag gtcataaaga tattaaaact accatgaata tctatgctca tgtcacacca
    12781 caatctgtta agaaaactgg tgaccgattt gcaaaattta tgggcgaata attccaaacg
    12841 tagccaaaaa cgtagccaac cattaaaata caaataaaaa aacgctcgaa acgctttaat
    12901 atcaacgttt cgagcaatga aatagttatt aacctacaga cccttccatc tcataactga
    12961 tcaatcgatt gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa
    13021 agcccatgac gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt
    13081 agagttgttc ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt
    13141 tgtggatctc attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt
    13201 cacattcgat atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat
    13261 agtttacttc tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg
    13321 gtgcattgtg gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga
    13381 tggagagcat ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg
    13441 ttgtttttgc acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg
    13501 ctcgtttggt tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc
    13561 aatacgcatc ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat
    13621 aagtaggtgc cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca
    13681 gcgtacgttc gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa
    13741 catcgacttt gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat
    13801 tcagcgctgc taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata
    13861 attcagggta ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga
    13921 attcatcttt catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta
    13981 gataagctcg ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg
    14041 gtacgtcttc ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat
    14101 cgaaatcgat gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt
    14161 ctagagattt cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct
    14221 cacggatcac ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt
    14281 gaaacccgaa tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg
    14341 tggtaatgat ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag
    14401 gcaagtgtcg cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca
    14461 tcgcctaatt ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca
    14521 gttaaatgtt ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca
    14581 cttgccgtac tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct
    14641 tttacatcga cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg
    14701 cttgtcgagt cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact
    14761 tgacggtata aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat
    14821 gattgcttca actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg
    14881 agcagtcgct gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct
    14941 gacggctacc ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa
    15001 attgaatgca acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc
    15061 atcaattgct tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat
    15121 attgtccata ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc
    15181 gatatttggt gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa
    15241 ttcgacaaaa tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt
    15301 tcgtttacca tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc
    15361 aaagaaatca cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc
    15421 atctactacg atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt
    15481 gaagacacct aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat
    15541 ctttttcgct gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa
    15601 gacagcacct gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat
    15661 gtaagagagc actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac
    15721 ccaattcaag cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg
    15781 gatgaacgcc cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa
    15841 cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg
    15901 gagtacttgt tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc
    15961 attgacgtct tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca
    16021 ttcaacttcc cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct
    16081 gttaagacag aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat
    16141 cctctactca tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt
    16201 ccagcagtta cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta
    16261 tcagaaagca tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag
    16321 atgtggccga taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa
    16381 atatgcccga tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt
    16441 ctgcccgagc tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct
    16501 gagtcaaagt cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa
    16561 gcatctcgta aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga
    16621 tctacggctg aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta
    16681 cctgatgatt ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca
    16741 ctatgttcat ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct
    16801 tgaatgaata aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa
    16861 ataccgctgt tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact
    16921 gcttttttca tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag
    16981 tcggtaaaaa tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat
    17041 gaaccttgtt ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct
    17101 gtttcactga tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc
    17161 cgatcgatat gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt
    17221 ctcatccaat caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg
    17281 agccagtttt tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt
    17341 cctaattctt tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca
    17401 ttccctgtca tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag
    17461 ttcaataatc gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca
    17521 tttactcctt tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct
    17581 aaaattgcaa atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct
    17641 ccgccagaaa aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa
    17701 gccatttttt catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca
    17761 cgtttcgcat tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt
    17821 ggatattgca tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa
    17881 acatttttcc cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg
    17941 atagcggcag ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct
    18001 ccggttttca ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt
    18061 agatttttta tttctaagac agacatgcat tataccccca aattctcttt cgttctcact
    18121 cattttagac taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt
    18181 tatttattat taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat
    18241 aagccattac agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt
    18301 tactggttta acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg
    18361 aagcacttca atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat
    18421 aatattcacg taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa
    18481 ccctgcgccg taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta
    18541 cgtagaagct aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc
    18601 ttcaatatca tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc
    18661 agcatcttgt agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat
    18721 tgttgcacca tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg
    18781 ttcgatgtgg acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt
    18841 caaataagga atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt
    18901 cggtaataca tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa
    18961 tggtttcaca tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt
    19021 cgtgtcgtct gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa
    19081 tcctacagtc agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat
    19141 ccccctatat cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga
    19201 acgataaata cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca
    19261 cgctggtagc cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc
    19321 atcgcagtaa atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga
    19381 atactttcag gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct
    19441 gctgcttcca gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa
    19501 aatggtgctg cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct
    19561 acgattgctt tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg
    19621 aaaatattac tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg
    19681 cgtccaatag agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt
    19741 gagattaatg tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt
    19801 tgtttaaaat ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc
    19861 tactccttgt tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat
    19921 caagacagtc aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac
    19981 gttgacatcg atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct
    20041 ttcgctaaag gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa
    20101 tagttctgtc gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc
    20161 ttttttcggt tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc
    20221 catcacagcg actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac
    20281 gatggtcaaa ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt
    20341 ttgcgggtcc aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa
    20401 tgctcgtgcg ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg
    20461 tccgcgtcct tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct
    20521 ctttggtacg ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca
    20581 taacaggttg aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt
    20641 gttttttaat tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc
    20701 aagtccattc aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc
    20761 ataaatatcc cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt
    20821 tttcccattg aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc
    20881 tttctcttat aatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg
    20941 aaagcttgaa cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt
    21001 ccagtacttg gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag
    21061 taagtttgct tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac
    21121 ccattttcag caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag
    21181 ctttctcgtt tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact
    21241 tttttcaagt gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga
    21301 aacttaagat ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga
    21361 ttttatcatt aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt
    21421 tttccgcttc aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca
    21481 gattggcaaa cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt
    21541 attcttttcc attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca
    21601 ttttatatca gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc
    21661 tctcgtaaga tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct
    21721 tttgaagccg taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg
    21781 cttgtgttga acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt
    21841 gaggccttcg gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct
    21901 aaccacgctt tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat
    21961 tttcttacct ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca
    22021 acccgatacc gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg
    22081 ggatccctgt taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca
    22141 gcagcatcat gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac
    22201 acacccgaat catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga
    22261 taaagccgaa gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat
    22321 agatattgct tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc
    22381 cttgggccgg ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa
    22441 tacgtgcaaa ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag
    22501 tcgtaaccaa gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga
    22561 tgatgcgcca agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa
    22621 gcatcgttcc aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca
    22681 agagcttccc aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat
    22741 gtttagttac gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct
    22801 ggaaagtaaa cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct
    22861 gatcatagaa tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag
    22921 tcagtttcca gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa
    22981 ctaaatacca gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat
    23041 ggctaagtgc tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa
    23101 tcacgccata atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc
    23161 ccttttctta aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc
    23221 tttccataga catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca
    23281 gtacaaaagc ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt
    23341 atctaccagc tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg
    23401 atctcttcgg caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa
    23461 aaatatcgat ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac
    23521 aaaatcgagc gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg
    23581 atcatcttct tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca
    23641 ccagagtttg cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc
    23701 tggtcttcca tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa
    23761 ccaaaatcgg attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg
    23821 atctcaaaat gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga
    23881 tagacagcaa aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg
    23941 agcaattttt gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat
    24001 aagtcggctt gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata
    24061 ttcaattccc gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt
    24121 acttctgctg ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac
    24181 ttaaacatgg caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt
    24241 aaatatacaa aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta
    24301 gtaacttaga tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt
    24361 ttacaatgta ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata
    24421 tatgaagaac tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc
    24481 ttcccttgga aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg
    24541 tatctctatg attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac
    24601 gaaacaaacg ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact
    24661 catggttatt tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat
    24721 ttggctgtgc gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt
    24781 tatcaaagct gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg
    24841 atttggggtc atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt
    24901 ttggatttca ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt
    24961 cgtaagaaat atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc
    25021 tctttgccta acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc
    25081 attgttcgct ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt
    25141 caagcttcgt ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac
    25201 agctatattt actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga
    25261 tttcttaaaa gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca
    25321 ttacgtacgt ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat
    25381 cgcttaataa tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg
    25441 gccaaacaaa aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa
    25501 gcttttgaac accgtttacc aagaggtcgt aa
//
LOCUS       DACEJZ010000037        24271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEJZ010000037 DACEJZ010000000
VERSION     DACEJZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.037"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..572
                     /locus_tag="IW656_001391"
     CDS             <1..572
                     /locus_tag="IW656_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0837103.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IW656_001392"
     CDS             576..1217
                     /locus_tag="IW656_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837104.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IW656_001393"
     CDS             1214..2131
                     /locus_tag="IW656_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0837105.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IW656_001394"
     CDS             2128..2799
                     /locus_tag="IW656_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837106.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IW656_001395"
     CDS             complement(3340..3828)
                     /locus_tag="IW656_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0837107.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IW656_001396"
     CDS             complement(4029..4295)
                     /locus_tag="IW656_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0837108.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IW656_001397"
     CDS             complement(4295..4558)
                     /locus_tag="IW656_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0837109.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IW656_001398"
     CDS             complement(4551..4823)
                     /locus_tag="IW656_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837110.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IW656_001399"
     CDS             5034..5219
                     /locus_tag="IW656_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0837111.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IW656_001400"
     CDS             complement(5361..7184)
                     /locus_tag="IW656_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0837112.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IW656_001401"
     CDS             complement(7782..10487)
                     /locus_tag="IW656_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0837113.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IW656_001402"
     CDS             complement(10793..11740)
                     /locus_tag="IW656_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0837114.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IW656_001403"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IW656_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0837115.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IW656_001404"
     CDS             complement(12375..12737)
                     /locus_tag="IW656_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0837116.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IW656_001405"
     CDS             complement(12748..13869)
                     /locus_tag="IW656_001405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0837117.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IW656_001406"
     CDS             complement(13885..14235)
                     /locus_tag="IW656_001406"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0837118.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IW656_001407"
     CDS             complement(14365..15876)
                     /locus_tag="IW656_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0837119.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IW656_001408"
     CDS             complement(16198..17550)
                     /locus_tag="IW656_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0837120.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IW656_001409"
     CDS             complement(17703..18779)
                     /locus_tag="IW656_001409"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0837121.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IW656_001410"
     CDS             18975..20720
                     /locus_tag="IW656_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0837122.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IW656_001411"
     CDS             complement(21000..21437)
                     /locus_tag="IW656_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0837123.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IW656_001412"
     CDS             complement(21421..22794)
                     /locus_tag="IW656_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837124.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IW656_001413"
     CDS             complement(23116..23604)
                     /locus_tag="IW656_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0837125.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IW656_001414"
     CDS             complement(23626..24228)
                     /locus_tag="IW656_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0837126.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DACEJZ010000038        23819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEJZ010000038 DACEJZ010000000
VERSION     DACEJZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.038"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..779)
                     /locus_tag="IW656_001415"
     CDS             complement(45..779)
                     /locus_tag="IW656_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0837127.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IW656_001416"
     CDS             1106..1516
                     /locus_tag="IW656_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0837128.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IW656_001417"
     CDS             1509..2210
                     /locus_tag="IW656_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0837129.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IW656_001418"
     CDS             2210..3796
                     /locus_tag="IW656_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0837130.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IW656_001419"
     CDS             3815..4036
                     /locus_tag="IW656_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0837131.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IW656_001420"
     CDS             4033..4794
                     /locus_tag="IW656_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0837132.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IW656_001421"
     CDS             5174..5683
                     /locus_tag="IW656_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0837133.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IW656_001422"
     CDS             complement(5758..6297)
                     /locus_tag="IW656_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0837134.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IW656_001423"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IW656_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0837135.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IW656_001424"
     CDS             7364..8161
                     /locus_tag="IW656_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0837136.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IW656_001425"
     CDS             8189..8824
                     /locus_tag="IW656_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0837137.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IW656_001426"
     CDS             8844..9617
                     /locus_tag="IW656_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0837138.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IW656_001427"
     CDS             10025..10822
                     /locus_tag="IW656_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0837139.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IW656_001428"
     CDS             10800..11213
                     /locus_tag="IW656_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837140.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IW656_001429"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IW656_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0837141.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IW656_001430"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IW656_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0837142.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IW656_001431"
     CDS             15990..16550
                     /locus_tag="IW656_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0837143.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IW656_001432"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IW656_001432"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0837144.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IW656_001433"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IW656_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0837145.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IW656_001434"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IW656_001434"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0837146.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IW656_001435"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IW656_001435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0837147.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IW656_001436"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IW656_001436"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0837148.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IW656_001437"
     CDS             21897..22475
                     /locus_tag="IW656_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0837149.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IW656_001438"
     CDS             22527..23009
                     /locus_tag="IW656_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0837150.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IW656_001439"
     CDS             23126..23500
                     /locus_tag="IW656_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837151.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7774 a         4284 c         5115 g         6646 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagtt
//
LOCUS       DACEJZ010000039        23657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEJZ010000039 DACEJZ010000000
VERSION     DACEJZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.039"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..530
                     /locus_tag="IW656_001440"
     CDS             255..530
                     /locus_tag="IW656_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837152.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IW656_001441"
     CDS             543..2621
                     /locus_tag="IW656_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0837153.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="IW656_001442"
     CDS             2781..4667
                     /locus_tag="IW656_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0837154.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IW656_001443"
     CDS             4679..5626
                     /locus_tag="IW656_001443"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0837155.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IW656_001444"
     CDS             5646..6173
                     /locus_tag="IW656_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0837156.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IW656_001445"
     CDS             complement(6236..6889)
                     /locus_tag="IW656_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0837157.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IW656_001446"
     CDS             7021..7863
                     /locus_tag="IW656_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0837158.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IW656_001447"
     CDS             8021..8917
                     /locus_tag="IW656_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0837159.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IW656_001448"
     CDS             8920..9633
                     /locus_tag="IW656_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0837160.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IW656_001449"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IW656_001449"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0837161.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IW656_001450"
     CDS             10168..10395
                     /locus_tag="IW656_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0837162.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IW656_001451"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IW656_001451"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0837163.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IW656_001452"
     CDS             complement(11181..11723)
                     /locus_tag="IW656_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0837164.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IW656_001453"
     CDS             complement(11960..12358)
                     /locus_tag="IW656_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0837165.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IW656_001454"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IW656_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0837166.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IW656_001455"
     CDS             13471..14238
                     /locus_tag="IW656_001455"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0837167.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IW656_001456"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IW656_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0837168.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IW656_001457"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IW656_001457"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0837169.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IW656_001458"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IW656_001458"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0837170.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18894..19799
                     /gene="xerC"
                     /locus_tag="IW656_001459"
     CDS             18894..19799
                     /gene="xerC"
                     /locus_tag="IW656_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0837171.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19836..20384
                     /gene="hslV"
                     /locus_tag="IW656_001460"
     CDS             19836..20384
                     /gene="hslV"
                     /locus_tag="IW656_001460"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0837172.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20398..21798
                     /gene="hslU"
                     /locus_tag="IW656_001461"
     CDS             20398..21798
                     /gene="hslU"
                     /locus_tag="IW656_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0837173.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21816..22607
                     /gene="codY"
                     /locus_tag="IW656_001462"
     CDS             21816..22607
                     /gene="codY"
                     /locus_tag="IW656_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0837174.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22689..23564
                     /locus_tag="IW656_001463"
     CDS             22689..23564
                     /locus_tag="IW656_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0837175.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6403 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacaccggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt cttgcgtttt tcttaagaat ttctcaaaca aaatgtgagg
    18781 aattgcgcca attgtgaaca aacttagaaa tattggttaa attctgacaa tttatttgtc
    18841 tttttataaa cgctgtgcta aattattctc tgggacaaaa ggaaggtgag aaaatgacag
    18901 aagccacgtg ggaacaaaaa tttttgcgtt atttgattgt cgaaagagga tattcagaaa
    18961 aaacgaaaga agcatatgaa gaagacattc tccatttcaa acattttttg gaggaatctg
    19021 gagacgctga tctgctgaag gcggaatatt ttacggttcg tgtctatttg agtgcattag
    19081 ctgatcatcg tcctagctat agcagaaatt ctatcagtcg caagatagcc agcctacgtt
    19141 cgttttatca gtatcttctc aaagaagaag tgataaaaga aaatccattt tcttacgttc
    19201 atctcaaaaa gaaaaatttg agactgccac gtttttttta tgaaaatgaa atgcaagcac
    19261 tttttgacag cgtagcagga gatagtcctt tggatttgag gaaccgcgct ttactggaag
    19321 tcctttacgg gtcaggcatc cgtttaagtg agtgtagcag tttgtcggta tcagacattg
    19381 attttgattc agaggtcatg ttgatccatg gtaaagggaa caaagagaga tatgctcctc
    19441 taggttcttt tgctcaagat gctctgcaag aatatttcag ggaaggccgc caggtattga
    19501 tgacaaaata tcacgaagag catgatatcg tttttgtgaa tcatcatgga aaaccaatca
    19561 cgcctactgg aattgaatat gtcttgaatc aaatgatcaa aaaaagcagt ttggatagtg
    19621 atatccatcc tcacatgctg cgccatacat ttgctactca tttattaaat aatggggcag
    19681 atttgcggac agtccaagaa ttgctaggac atgccaattt atcgactacc cagatctatg
    19741 cccatgtaac aaaagaaagt ttacaaaaaa actatcgttc tttccatcca agagcgtaaa
    19801 aataaggaaa agaacaaatt taggaggaaa caaacatggt cgaatcacca tttcattcaa
    19861 ccactatctg tgccgtcgaa aaagacggga aatttgctat ggctggagat ggccaagtaa
    19921 caatgggtga atctgttgtc atgaaaggca ctgcaaagaa agtacgacgt atctacaatg
    19981 gagaagttgt tgtaggtttc gccggaagtg ttgcagatgc gtttacttta gaagaaaaat
    20041 ttgaaggcaa actaaacgaa tataacggga atctacaacg cgcagctgtc gaattagctc
    20101 aagaatggcg gacacagcaa tctatgcaaa aacttgaagc gatgctgatc gtaatgaatg
    20161 ataaagaaat gcttcttgtt tcaggtacag gagaagttat cacaccagat gatggtatct
    20221 tagctatcgg atcaggaggg aattttgcgt tggctgcagc ccgagcaatg aaaaactatg
    20281 ctcaaaacga catgagtgcg aaagaaattg cagaaaatgc gttaaatatt gccgcagata
    20341 tctgtatctt tactaatcat aatattatcg tagaagaact gtaagaggtg agtagatatg
    20401 tatgaattaa aacaaacacc aagacaaatc gtaagtgaat tagatgaata tatcgtagga
    20461 cagcacgcag caaaaaaatc tgttgccgtt gctttgcgta accgctatcg ccgtttgcag
    20521 ttagatgaaa aaatgcaaca agatattaca ccaaaaaata tgctgatgat cggacctaca
    20581 ggggtcggta aaacagaaat cgcacgacgt ttggcaaaaa tcgtcaatgc accatttgtc
    20641 aaagtcgaag caacaaaatt tactgaagtc ggttatgtcg gaagagatgt agaatcaatg
    20701 gttcgggatt tagtagaaaa tgcgatccaa atcgtagaaa aagagcagta cagccgtgta
    20761 tacacaagtg ctaaaaaaaa tgcagaaaaa cgtttggtaa aaatcttggt acctggtatc
    20821 aaaaaagaac agaaacaatc cactaatcaa tttgaacaaa tgatgaatct ttttaatgct
    20881 agtcagcaaa ataccgctga accgcaagaa gaagtgacag aagatattcg aatcaatcgc
    20941 cgtacagtct tggaacaact acaaaagggt ttactggata gtcgtgaagt aaccattgaa
    21001 gttgaagacc aaaagaaacc tgcaccaatg atgaacaatg gattggagca aatggggatc
    21061 gacctaaatg aaactctagg tgctttgaca ccgaagaaaa aagtggaacg gacggtaaca
    21121 gtcaaagaag ctttagagtt attgatcaag gaagaatcag caaaattagt caaggatgct
    21181 gatatccata gtgaggcaat caaattagcg gaagaaagtg gaattatttt tatcgatgaa
    21241 atcgataaga tcacttctaa atcacagcaa tctggagaag tatccagaga aggcgtccaa
    21301 agagacatat tgccaattgt cgaaggttca caagtaaaca caaaatacgg gatcatccaa
    21361 acagatcacg tcctattcat tgcttcagga gctttccatt tgtctaaacc aagtgatcta
    21421 attccagaac ttcaaggacg cttcccgata cgggtagaat tagatgattt gacagctgaa
    21481 gattttgtga agattctgac cgaaccaaat aatgctttag taaaacaata cgtcgcgttg
    21541 atcggtacag aaaatgtgtc agttacgttt acaaaagaag caattgaacg gatcgctcaa
    21601 atcgctttcg atgtcaaccg tgacacagat aatatcggcg cacgcagact gcataccatt
    21661 ttggaaaaac tactagaaga tctattattt gaagcaccag atatgcaaat gggagaaata
    21721 acgatcacag aagcatatgt ggatgacaaa cttggctcaa ttgcggcaaa tgaagattta
    21781 agtcgctata ttttataaaa aatcggagga cgacaatgac agacttgcta acgaaaaccc
    21841 gcaagataaa cacacttttg cagcaaaaga atacttttga catgcaagca gatttgcctt
    21901 acgataaaat ggctgtcaca ctaggtggta ttcttgacag caacacctat atcataggaa
    21961 gtgacggagt attactaggt tataatgaac gtcacgatgt gaacaatgaa cgaatcaaaa
    22021 caatgtttgt tgagaaacaa tttcctgaat cttatactga tacagtggca cagttgatga
    22081 aaactgaagc taatatccca attactagtg atattactgc atttccggta gagtcaagag
    22141 agaaatatcc atttggtctg acgactgtcg ttccgatctt tggagctggc gaacgtttgg
    22201 gtacgattat cttatctcgt atggaacaat cgtttgcaga tgacgatcta gtcttagcag
    22261 aatatggagc tacggttgtc ggtatgcaga tcctttatca gaagtctcga agcatcgaag
    22321 cagatgtacg gagtgcaacg gctgtccaaa tggcaatcaa cacattatct tatagcgaat
    22381 tgaaggctgt acaagctatc tttgaagcat tggatggaga tgaaggtcgt ctgactgctt
    22441 ctaatattgc cgatagtatc ggcatcactc gttctgtgat cgtcaatgct ttacgaaaat
    22501 tggaatcagc tggaatcatt gaatctcgtt cgttaggcat gaagggaact tatttaaaag
    22561 tattgaattc ccgttttaaa gacgagttag caaaacacag ttattagaag taaggggact
    22621 ggggcagata gaaatgtttg ttccagcctt cccattttaa aaagtaagtc gaagttggag
    22681 gaatggcaat gactgttatt attcaaaatg accagctgat cgccgaaatc gctgaacacg
    22741 gtgcggaatt gatcagtctg aaatcaaaag aacatggttt agagtatatc tggcaaggtg
    22801 atccagcgta ttggggcaga catgcaccgg ttttattccc ttttgttgga cgtttaaaag
    22861 atgatcagta tgtttatcaa gggaaaacat atcaaatggg gcaacacgga tttgctcggg
    22921 atatggattt tgaagtcatt gaacatggac aagaaatggc tagtttcatc ttaaggagta
    22981 cgccggagac attagaaaag tatccatttg attttgaatt agtgatcagc tatgaattag
    23041 gaggagacgg catcacggtt cattatcaag tggcaaatac cggtgaaaaa gaaatgttct
    23101 tttctatcgg tggacatcct gctttcaatg tacctcttga agaaggattg tcatttgaag
    23161 atttctatct ggcattttct ccattgaaat caagattacg cctaccattg aaaggcccat
    23221 tcatcgatat ggatcaaaag acgttaggtc agacaaatac gaatctggct ttgaatcgtg
    23281 aactttttga gcaagatgcc ttggtttttg aaacaagggg tctaaatgcg tacgccattc
    23341 gaagtgaaaa atcttcccat agcgtaactt taagctataa agatatgcct tatgtaggga
    23401 tctggtctcc ttatccaaaa gaagcgcctt tcgtttgtat cgaaccgtgg tgcggaatcg
    23461 ctgacacggt gaacagtagt ggaaacttga tggaaaaagt agggatcaat caactaggtg
    23521 cccatgaaat cttcaagaca aaatattcca tcacagtgaa gtaagaggtt gtgaccgaga
    23581 agttagcagg gaacaccgtt taccaaggtg tgtaaaaaag ctatcctgta agtaataagg
    23641 gagtgtgaca tcgattt
//
LOCUS       DACEJZ010000040        23646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEJZ010000040 DACEJZ010000000
VERSION     DACEJZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.040"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..171
                     /locus_tag="IW656_001464"
     CDS             <1..171
                     /locus_tag="IW656_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0837176.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IW656_001465"
     CDS             346..642
                     /locus_tag="IW656_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837177.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IW656_001466"
     CDS             649..2601
                     /locus_tag="IW656_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0837178.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IW656_001467"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IW656_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0837179.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3532..4572
                     /locus_tag="IW656_001468"
     CDS             3532..4572
                     /locus_tag="IW656_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0837180.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4670..4858
                     /locus_tag="IW656_001469"
     CDS             4670..4858
                     /locus_tag="IW656_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0837181.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4916..5269)
                     /locus_tag="IW656_001470"
     CDS             complement(4916..5269)
                     /locus_tag="IW656_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837182.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5800..6222
                     /locus_tag="IW656_001471"
     CDS             5800..6222
                     /locus_tag="IW656_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0837183.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6219..6449
                     /locus_tag="IW656_001472"
     CDS             6219..6449
                     /locus_tag="IW656_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837184.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6947..7147
                     /locus_tag="IW656_001473"
     CDS             6947..7147
                     /locus_tag="IW656_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0837185.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7174..8367
                     /locus_tag="IW656_001474"
     CDS             7174..8367
                     /locus_tag="IW656_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0837186.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IW656_001475"
     CDS             complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IW656_001475"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0837187.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10059..10982)
                     /locus_tag="IW656_001476"
     CDS             complement(10059..10982)
                     /locus_tag="IW656_001476"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0837188.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11178..11783
                     /locus_tag="IW656_001477"
     CDS             11178..11783
                     /locus_tag="IW656_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0837189.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12091..13107
                     /locus_tag="IW656_001478"
     CDS             12091..13107
                     /locus_tag="IW656_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0837190.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13248..14549
                     /locus_tag="IW656_001479"
     CDS             13248..14549
                     /locus_tag="IW656_001479"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0837191.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14595..15257
                     /gene="deoC"
                     /locus_tag="IW656_001480"
     CDS             14595..15257
                     /gene="deoC"
                     /locus_tag="IW656_001480"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0837192.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15278..15676
                     /locus_tag="IW656_001481"
     CDS             15278..15676
                     /locus_tag="IW656_001481"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0837193.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15742..16824
                     /locus_tag="IW656_001482"
     CDS             15742..16824
                     /locus_tag="IW656_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0837194.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17036..18598
                     /locus_tag="IW656_001483"
     CDS             17036..18598
                     /locus_tag="IW656_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837195.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18591..19724
                     /locus_tag="IW656_001484"
     CDS             18591..19724
                     /locus_tag="IW656_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837196.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19724..20680
                     /locus_tag="IW656_001485"
     CDS             19724..20680
                     /locus_tag="IW656_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837197.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20779..21945
                     /gene="deoB"
                     /locus_tag="IW656_001486"
     CDS             20779..21945
                     /gene="deoB"
                     /locus_tag="IW656_001486"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0837198.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21969..22787
                     /locus_tag="IW656_001487"
     CDS             21969..22787
                     /locus_tag="IW656_001487"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0837199.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22804..23511
                     /gene="deoD"
                     /locus_tag="IW656_001488"
     CDS             22804..23511
                     /gene="deoD"
                     /locus_tag="IW656_001488"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0837200.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7688 a         4188 c         4933 g         6837 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
     4141 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
     4201 cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta
     4261 gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac
     4321 gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt
     4381 attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt
     4441 acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa
     4501 cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat
     4561 aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga
     4621 aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta
     4681 caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac
     4741 tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt
     4801 tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt
     4861 tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc
     4921 cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt
     4981 tgtctgtaaa gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat
     5041 tatctgtagg taagaaaaat tcatcaatag atacatggag taatgataca agatcataca
     5101 gcacttgtgt acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa
     5161 tttcaatcat tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa
     5221 tcgctaaacc aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc
     5281 tctatacatt ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag
     5341 tttatgagtt cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata
     5401 gaaaggtcgc tgattttatt tacctataca actttataaa atttagtcat actcctagaa
     5461 agtagataaa attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca
     5521 tctagttctc ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat
     5581 gaccatcaat gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga
     5641 aaacaatctg tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc
     5701 atttatgcaa caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca
     5761 catgccactc cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa
     5821 gaaaccatag aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa
     5881 gattacttca aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat
     5941 aactaccttg tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt
     6001 aaattatttg gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat
     6061 ctttcagaga aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg
     6121 gagattgcgg agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga
     6181 ctagccatga ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg
     6241 ttcccttccc cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg
     6301 ttaaacatta tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg
     6361 gaaattctca tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct
     6421 ctaagatttt atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg
     6481 tatccacgct tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt
     6541 aggttttgat aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata
     6601 cgtttcggta agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg
     6661 attcatgtta gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg
     6721 tgataatgat actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt
     6781 acggctacat gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga
     6841 ttgctaagat attcttctct tgtcagtgta ttattataaa actatttgat ataattatag
     6901 gtgaatattg gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac
     6961 aaaaaaggat ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa
     7021 agcaaaagaa ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag
     7081 agtccctcgt gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc
     7141 tgagtagttt ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca
     7201 aagaatggaa cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt
     7261 caaaaatatc gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg
     7321 ctattgatga aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat
     7381 atctttgaaa ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat
     7441 cgcttaccaa ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt
     7501 gagcctattg atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat
     7561 gtaagagctg ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata
     7621 accaccacaa atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa
     7681 ttttggacaa aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat
     7741 caacactttt tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa
     7801 gctactgcca ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa
     7861 acactctatt acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc
     7921 gttcgtcata ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta
     7981 caacaaaatc taattcagac agactttgta atgagctata acggtattcc tacccaaaaa
     8041 catacactat catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga
     8101 atccatgggt tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt
     8161 atcattaaag atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat
     8221 ctttatccta acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa
     8281 gaagcaaaac aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga
     8341 aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac
     8401 ccttggtata agaggatttc tagctctcct acaactattc ccactccact gttgctggtg
     8461 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
     8521 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
     8581 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
     8641 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
     8701 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
     8761 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
     8821 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
     8881 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
     8941 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
     9001 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
     9061 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
     9121 acttgtctaa gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc
     9181 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
     9241 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
     9301 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
     9361 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
     9421 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
     9481 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
     9541 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
     9601 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
     9661 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
     9721 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
     9781 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
     9841 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
     9901 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
     9961 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    10021 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    10081 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    10141 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    10201 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    10261 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    10321 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    10381 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    10441 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    10501 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    10561 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    10621 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    10681 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    10741 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    10801 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    10861 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    10921 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    10981 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    11041 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    11101 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    11161 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    11221 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    11281 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    11341 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    11401 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    11461 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    11521 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    11581 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    11641 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    11701 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    11761 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    11821 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    11881 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    11941 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    12001 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    12061 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    12121 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    12181 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    12241 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    12301 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    12361 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    12421 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    12481 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    12541 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    12601 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    12661 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    12721 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    12781 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    12841 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    12901 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    12961 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    13021 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    13081 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    13141 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    13201 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    13261 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    13321 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    13381 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    13441 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    13501 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    13561 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    13621 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    13681 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    13741 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    13801 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    13861 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    13921 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    13981 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    14041 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    14101 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    14161 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    14221 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    14281 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    14341 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    14401 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    14461 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    14521 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    14581 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    14641 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    14701 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    14761 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    14821 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    14881 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    14941 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    15001 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    15061 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    15121 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    15181 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    15241 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    15301 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    15361 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    15421 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    15481 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    15541 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    15601 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    15661 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    15721 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    15781 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    15841 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    15901 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    15961 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    16021 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    16081 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    16141 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    16201 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    16261 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    16321 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    16381 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    16441 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    16501 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    16561 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    16621 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    16681 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    16741 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    16801 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    16861 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    16921 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    16981 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    17041 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    17101 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    17161 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    17221 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    17281 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    17341 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    17401 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    17461 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    17521 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    17581 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    17641 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    17701 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    17761 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    17821 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    17881 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    17941 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    18001 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    18061 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    18121 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    18181 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    18241 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    18301 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    18361 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    18421 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    18481 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    18541 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    18601 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    18661 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    18721 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    18781 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    18841 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    18901 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    18961 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    19021 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    19081 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    19141 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    19201 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    19261 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    19321 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    19381 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    19441 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    19501 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    19561 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    19621 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    19681 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    19741 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    19801 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    19861 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    19921 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    19981 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    20041 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    20101 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    20161 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    20221 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    20281 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    20341 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    20401 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    20461 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    20521 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    20581 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    20641 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    20701 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    20761 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    20821 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    20881 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    20941 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    21001 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    21061 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    21121 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    21181 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    21241 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    21301 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    21361 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    21421 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    21481 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    21541 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    21601 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    21661 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    21721 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    21781 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    21841 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    21901 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    21961 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    22021 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    22081 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    22141 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    22201 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    22261 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    22321 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    22381 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    22441 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    22501 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    22561 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    22621 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    22681 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    22741 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    22801 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    22861 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    22921 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    22981 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    23041 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    23101 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    23161 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    23221 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    23281 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    23341 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    23401 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    23461 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    23521 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    23581 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    23641 tgtgaa
//
LOCUS       DACEJZ010000041        23573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEJZ010000041 DACEJZ010000000
VERSION     DACEJZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.041"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IW656_001489"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IW656_001489"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0837201.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IW656_001490"
     CDS             complement(1174..1869)
                     /locus_tag="IW656_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0837202.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IW656_001491"
     CDS             complement(1882..2598)
                     /locus_tag="IW656_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0837203.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IW656_001492"
     CDS             2928..3533
                     /locus_tag="IW656_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0837204.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IW656_001493"
     CDS             3858..4181
                     /locus_tag="IW656_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0837205.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IW656_001494"
     CDS             4363..5733
                     /locus_tag="IW656_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0837206.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IW656_001495"
     CDS             complement(5863..7164)
                     /locus_tag="IW656_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0837207.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IW656_001496"
     CDS             complement(7394..9385)
                     /locus_tag="IW656_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0837208.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IW656_001497"
     CDS             complement(9529..9795)
                     /locus_tag="IW656_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0837209.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IW656_001498"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IW656_001498"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0837210.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IW656_001499"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IW656_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0837211.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IW656_001500"
     CDS             13139..13702
                     /locus_tag="IW656_001500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0837212.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IW656_001501"
     CDS             complement(13955..14476)
                     /locus_tag="IW656_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0837213.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IW656_001502"
     CDS             complement(14479..15321)
                     /locus_tag="IW656_001502"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0837214.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IW656_001503"
     CDS             complement(15314..16039)
                     /locus_tag="IW656_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0837215.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IW656_001504"
     CDS             16215..16511
                     /locus_tag="IW656_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837216.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IW656_001505"
     CDS             17079..18671
                     /locus_tag="IW656_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0837217.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IW656_001506"
     CDS             18684..19424
                     /locus_tag="IW656_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837218.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IW656_001507"
     CDS             19592..20941
                     /locus_tag="IW656_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0837219.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IW656_001508"
     CDS             complement(21069..21659)
                     /locus_tag="IW656_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0837220.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IW656_001509"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IW656_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0837221.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6751 a         4746 c         4490 g         7586 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaata
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaa
//
LOCUS       DACEJZ010000042        23067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEJZ010000042 DACEJZ010000000
VERSION     DACEJZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.042"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            493..1200
                     /locus_tag="IW656_001510"
     CDS             493..1200
                     /locus_tag="IW656_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0837222.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IW656_001511"
     CDS             complement(1259..1903)
                     /locus_tag="IW656_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0837223.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IW656_001512"
     CDS             2030..2317
                     /locus_tag="IW656_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837224.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IW656_001513"
     CDS             2425..2745
                     /locus_tag="IW656_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0837225.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IW656_001514"
     CDS             2826..4139
                     /locus_tag="IW656_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0837226.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IW656_001515"
     CDS             4136..4855
                     /locus_tag="IW656_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0837227.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IW656_001516"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IW656_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0837228.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IW656_001517"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IW656_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0837229.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IW656_001518"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IW656_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0837230.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IW656_001519"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IW656_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0837231.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IW656_001520"
     CDS             10292..11185
                     /locus_tag="IW656_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837232.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IW656_001521"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IW656_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0837233.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IW656_001522"
     CDS             13122..13979
                     /locus_tag="IW656_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0837234.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IW656_001523"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IW656_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0837235.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IW656_001524"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IW656_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0837236.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IW656_001525"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IW656_001525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837237.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IW656_001526"
     CDS             16735..17493
                     /locus_tag="IW656_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837238.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IW656_001527"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IW656_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0837239.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IW656_001528"
     CDS             18406..19437
                     /locus_tag="IW656_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0837240.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IW656_001529"
     CDS             19434..20201
                     /locus_tag="IW656_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0837241.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IW656_001530"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IW656_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0837242.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IW656_001531"
     CDS             22048..22353
                     /locus_tag="IW656_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0837243.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IW656_001532"
     CDS             22353..22703
                     /locus_tag="IW656_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0837244.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22841..>23067
                     /locus_tag="IW656_001533"
     CDS             22841..>23067
                     /locus_tag="IW656_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837245.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAH"
BASE COUNT         7597 a         3972 c         4964 g         6534 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 ttatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    22801 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    22861 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    22921 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    22981 aggtaagact gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtgggag
    23041 aaaagtcatt caattacctg ctcatga
//
LOCUS       DACEJZ010000043        23060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEJZ010000043 DACEJZ010000000
VERSION     DACEJZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.043"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..445)
                     /locus_tag="IW656_001534"
     CDS             complement(161..445)
                     /locus_tag="IW656_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837246.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IW656_001535"
     CDS             complement(463..1353)
                     /locus_tag="IW656_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0837247.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IW656_001536"
     CDS             complement(1373..1591)
                     /locus_tag="IW656_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837248.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IW656_001537"
     CDS             complement(1561..3261)
                     /locus_tag="IW656_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0837249.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IW656_001538"
     CDS             3528..4301
                     /locus_tag="IW656_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837250.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW656_001539"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW656_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0837251.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IW656_001540"
     CDS             complement(5125..6096)
                     /locus_tag="IW656_001540"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0837252.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW656_001541"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW656_001541"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0837253.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW656_001542"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW656_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0837254.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IW656_001543"
     CDS             complement(9051..9875)
                     /locus_tag="IW656_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0837255.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IW656_001544"
     CDS             complement(9848..10528)
                     /locus_tag="IW656_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837256.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IW656_001545"
     CDS             complement(10551..11489)
                     /locus_tag="IW656_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0837257.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IW656_001546"
     CDS             complement(11734..12585)
                     /locus_tag="IW656_001546"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0837258.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW656_001547"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW656_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0837259.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IW656_001548"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IW656_001548"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0837260.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW656_001549"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW656_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0837261.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW656_001550"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW656_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0837262.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IW656_001551"
     CDS             complement(19463..20704)
                     /locus_tag="IW656_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0837263.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW656_001552"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW656_001552"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0837264.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IW656_001553"
     CDS             complement(21731..22615)
                     /locus_tag="IW656_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837265.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         6562 a         5019 c         3990 g         7489 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt
//
LOCUS       DACEJZ010000044        22804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEJZ010000044 DACEJZ010000000
VERSION     DACEJZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.044"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..427
                     /locus_tag="IW656_001554"
     CDS             <1..427
                     /locus_tag="IW656_001554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837266.1"
                     /translation="FCECVVVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLD
                     KISNIDDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPT
                     TDTSGEKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            508..1128
                     /locus_tag="IW656_001555"
     CDS             508..1128
                     /locus_tag="IW656_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837267.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1458..2684)
                     /locus_tag="IW656_001556"
     CDS             complement(1458..2684)
                     /locus_tag="IW656_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0837268.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2756..2914)
                     /locus_tag="IW656_001557"
     CDS             complement(2756..2914)
                     /locus_tag="IW656_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837269.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3602..4342
                     /locus_tag="IW656_001558"
     CDS             3602..4342
                     /locus_tag="IW656_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837270.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4434..5621)
                     /locus_tag="IW656_001559"
     CDS             complement(4434..5621)
                     /locus_tag="IW656_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0837271.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5943..6785
                     /locus_tag="IW656_001560"
     CDS             5943..6785
                     /locus_tag="IW656_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0837272.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6782..7732
                     /locus_tag="IW656_001561"
     CDS             6782..7732
                     /locus_tag="IW656_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0837273.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7747..7980
                     /locus_tag="IW656_001562"
     CDS             7747..7980
                     /locus_tag="IW656_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0837274.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8311..8895
                     /locus_tag="IW656_001563"
     CDS             8311..8895
                     /locus_tag="IW656_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0837275.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            9026..9694
                     /locus_tag="IW656_001564"
     CDS             9026..9694
                     /locus_tag="IW656_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0837276.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9696..11120
                     /locus_tag="IW656_001565"
     CDS             9696..11120
                     /locus_tag="IW656_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0837277.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            11138..11707
                     /locus_tag="IW656_001566"
     CDS             11138..11707
                     /locus_tag="IW656_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0837278.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11817..13571
                     /locus_tag="IW656_001567"
     CDS             11817..13571
                     /locus_tag="IW656_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837279.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13525..15297
                     /locus_tag="IW656_001568"
     CDS             13525..15297
                     /locus_tag="IW656_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837280.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            15820..16089
                     /gene="rpsN"
                     /locus_tag="IW656_001569"
     CDS             15820..16089
                     /gene="rpsN"
                     /locus_tag="IW656_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0837281.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16104..16283
                     /gene="rpmF"
                     /locus_tag="IW656_001570"
     CDS             16104..16283
                     /gene="rpmF"
                     /locus_tag="IW656_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0837282.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16315..16692
                     /locus_tag="IW656_001571"
     CDS             16315..16692
                     /locus_tag="IW656_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837283.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16713..16862
                     /gene="rpmG"
                     /locus_tag="IW656_001572"
     CDS             16713..16862
                     /gene="rpmG"
                     /locus_tag="IW656_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0837284.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16886..17869
                     /locus_tag="IW656_001573"
     CDS             16886..17869
                     /locus_tag="IW656_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0837285.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17899..18021
                     /locus_tag="IW656_001574"
     CDS             17899..18021
                     /locus_tag="IW656_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0837286.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            18039..19562
                     /locus_tag="IW656_001575"
     CDS             18039..19562
                     /locus_tag="IW656_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0837287.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19586..19825
                     /locus_tag="IW656_001576"
     CDS             19586..19825
                     /locus_tag="IW656_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837288.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(20018..20479)
                     /locus_tag="IW656_001577"
     CDS             complement(20018..20479)
                     /locus_tag="IW656_001577"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0837289.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20674..20892)
                     /locus_tag="IW656_001578"
     CDS             complement(20674..20892)
                     /locus_tag="IW656_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837290.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            21010..22626
                     /locus_tag="IW656_001579"
     CDS             21010..22626
                     /locus_tag="IW656_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0837291.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
BASE COUNT         7892 a         3269 c         4197 g         7446 t
ORIGIN      
        1 tttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt tttctattta
       61 cacaaaatat tttacactct cgtcaaatat aaaaactagt acgaaattaa tttgtaaatt
      121 atttaagctt gataaaataa gcaatatcga tgatgtaaag tccaagatat taacaagaaa
      181 agtaagcgaa ccagtaaagc taacggcaca agataaaaaa aatggaattg ttctttatgg
      241 tgatgatgca aagtataact ataattggga tacctcctca gaagctgagg tattaagtat
      301 tcctactacc gatacttcag gggaaaaagt cacctttaaa ataaatttta taacggcaat
      361 ttctaagctg gagtatggga agcaaaaaaa gagagataac aatgatcaat atctacctaa
      421 aaaataacga attaatgatg catatgttaa agttgttttt ttgaattaaa caactcaatt
      481 tattcagtta tatgttcatc gcagcaagtg aacattaatc gagttataaa attaatagga
      541 gctaaaaata taaataaagt tcgatcagaa tttatttttc cagaagattt ttttaactgg
      601 tttttttatc aattcagtga atgcaagcga ctcttaggtg agaattatta cttaaaagga
      661 atcggcggat ttatggggaa tgtggctgat gaacaaaata ttataaaagg aatatctgaa
      721 caaacatcag agcttacagt aactaaagca tttataagta atggtgaaag tttgaaaaat
      781 gtaactgcgc gaattagaaa agaaaatgaa gtagaaattg tttttggaat tgaccatgaa
      841 tgtaattcag tgatatatgt aacacaatca gcaaatttaa ttctttttga gaatggttgc
      901 aaggacgatt ttatggtggt ctatttgtac gatatactaa ttccaaaact tcgttcctta
      961 tatatgtctt ctgaaagtat ttttgtgagt gaacaaaagg gatctttctc gaaaaaaata
     1021 ggtattgaag taattgaatc gattatgtgt cataatcata ttacgctaga agaattagaa
     1081 attttgatag ggcaaaaaaa taataacacg aaaatacaaa tttcttagaa atcatttttt
     1141 attgagatat ctagataaat gatttattct tcaatcaacc aactcgccaa gctgaggtaa
     1201 tgttttgagg taatgataac atacaacttt ttatacccta gtaaaactct ataaacaaag
     1261 acttattaat ttcgcatgaa atacattcct gtaaaatgca ataaaataga gttttgttta
     1321 accaggtctc aaaaaggctc gtaaagcatc tcaattctct aaacgttaat attatttata
     1381 tatcaatgtt tcaaggcact tttcaattgt ttgaaaagtg tctttttctt gtttaagaac
     1441 acatttaaga acacatacta atcacattta ttcaatcctc tctgaaaggc ttcaaaaaca
     1501 gttttatcta ctacatctgg tgtatttacg tatattctcg ttgtagaaat atctgagtgt
     1561 gttaaagcac tagatatttg attcatattt gctccgcctt gtctagctat agttgcatag
     1621 gtatgacgaa gcttatgtgg agataaatga gccaattcag gatgtcgtct tttaattgaa
     1681 ttgattcgat agtttaggta atcagcgtga acagggcaat ttacttctcc ggatggttta
     1741 gtataggtaa agagatactg atctggtgtt tgattaattt ttaatctcaa caattgctca
     1801 gcttgcactg atttccattt gcttagtaat gccattacaa tttcgggtac ttgaattacg
     1861 gtatcctttc gaccttttgt aaatttcttt ttttgatatt tatccaaagc atgtttaagg
     1921 cgaaccgttt gtttctcaaa gtcgatatat ttccattgca aagcatacgt ctcacttttt
     1981 ctatcaccaa gatatagggt aagcatgaaa agagtatagt catgaagagt aagcaagtga
     2041 ttattcaagt cagtgtttac tgcttctatc caagcaataa gctcttctga cgataatgct
     2101 tgattacctt cccgtatcct tctttcttct aaagcaattt ttttaggtgc agtgatacat
     2161 tgaataattt tggtagttcg gttaaattca atataattta gtatttctgc aatatcaaac
     2221 atagaacgga catatccttt gataattttt atatttgcgt attctttcga tttttttgtt
     2281 aattcatctg atattatctc agtatttgaa tttagatagt tcattgcata ctcaccaaac
     2341 attggaagga tgtgcaatcg gaatatatct ttagtattac taattgttac gtaacttgga
     2401 gctctttttg atctagtagt ctgacctagt tcatatctag gaagccattt tgattcatag
     2461 aattccttaa attttatttt tccttttaat tctagtgaat ttgcattctc ctctcttaaa
     2521 actgtttcaa ttttcctttt cattgcattt tcggctttaa tagccaattg cctagttgga
     2581 aatacttttt gatacttttc tgatgtaatt ccagtgattt ctctgaattt ttttggatac
     2641 ctcagattag ttttatattt cccatttgaa agttttgtag gcaaaattcc tcactccctt
     2701 aaaaagtaag gcagtatatt tcgctattct tagtatatgc ggcgagaaga aattttcaaa
     2761 ttgttttaaa tgtattcata aaatctttga tatcctctat gtcaaagcga actataccgt
     2821 caattctgat tactggaaaa ttatatatac gaatccactt atctaaagta ttattatcaa
     2881 ttcctagaaa ctcacaggtt tgttttttat tcaagtagac tctcaatttt ataaaatcat
     2941 ctcctaaaaa agattagaat ttttgaattg ctttgggtaa gcacatagga atttcacctc
     3001 tccccagttt acggcgagcc ttacggaagt atcattattt gctgctattg tcatggcatt
     3061 ttgttttccc agaacaaaaa gaaatctcta agtatttgtc gctaatcagg tcatggcgta
     3121 cttgagtttc tagctcgaat agcagggatc gttattcaaa aattgtattt aattgtcaat
     3181 gatcaaaaat aggttttttc ctattcacta ttaataacga taaacatgta taaaaagaca
     3241 cactacgaaa aaagttaaat ggagtttact gattttcata tttgtatatg gaacagacat
     3301 agtcttgaat aatttttata aatgttttga tgcttatcaa cttattcaac ccgctatcca
     3361 ctttaagtat aatttttcca tacgccaata aagtagtttt aggagcatgt tctcatttga
     3421 gaacgctcct tttttatata ttttgaaagc tacttactat acgcgtagtt tcaaatagtt
     3481 ttgtcattct aatcttcagc aacagaaaaa aatctaaatt ataaaattta gggttgacta
     3541 aaatataatt tttgtatacc atatgatcgt gataatcatt atcatttaga aggagaaagt
     3601 tatgttagaa gtgaaaaagc ttaatgtttg ttataacgat tttttagcat tgaacgagat
     3661 ttctttaaaa atacaagaag gtgctttaac aggtattatt ggtccaaatg gtgctggaaa
     3721 atctacattg cttaaagcta tgttaaatat tatccctcat cagggagagg tctccattaa
     3781 taatgaagat atcaataaaa aactggtgaa gattgcatat gtagagcaaa aagctgatat
     3841 tgattttact tttccaataa aagtaaaaga atgtgtatct atgggaacat atgccggaat
     3901 gaaagttttt caaagaatta aaaatgcaga atggcaaaga gtttcaaagg ctttggaaaa
     3961 ggtcgatatg ggaaaatatt ctaatcatca aataggagaa ttatctggag gacaatttca
     4021 acgtgtactt ttagcaagat gtcttgctca aaatgcggat ttcatatttt tagatgaacc
     4081 atttgtaggg atagacttag ttagtgaacg aataattatg gatacgttaa aagaattgaa
     4141 acatcaggga aaaacagtgc taattgtgca tcatgattta agcaaggtaa agaagtattt
     4201 tgataatatt attcttttga atcgaaatct aattgctcac ggaagtgtag aatctgtatt
     4261 taatgaagag aatttaaaaa aagcctatgg agatactata tttattggag agactaacta
     4321 tatagagtca agtaaaaatt agccaaatca aaaaatattt tataacagat aattccgaaa
     4381 aaagggtaca acaaataagc ttcttaccat tagttttttt gaatgttcaa tagctaatca
     4441 gaaacaatta tataaggctc gtttttagtc aaaatagcat gaatcacgta acaaagctta
     4501 cgagaaaccg cacctaaggc agttagatga tgttttcctt cggaaacttt tttctggtaa
     4561 aacgcactaa aaacaggatc acattgagaa gctactaaag ccgccgaaaa caaggctttt
     4621 cgcaaatatg gagaccctcg tttactcata acattatgcg ttgcctcata ttgccctgat
     4681 tgtgtcacgg aagcgtctat cccagcgtaa gcaacgagtt ttttcgggtt agaaaatttt
     4741 tgaatatctc cgatctcacc aagaatagtc gcaccgttta taggaccaat tccaggaatt
     4801 gtgagaataa cagaatttaa agtgtccatt atgtgcttga tttctctttc cgtttccttt
     4861 atttggcttt caatgaattt taattgttca atcattgaac gtagttggaa ggtgaacgct
     4921 tcttgagcaa attttactcc aaaggagtta gaagctgctt gcttcaattg gtccgctttt
     4981 tctgccttga ttcggttcct gcttaactca gctaagtatt gggccaaagt ttctgaggaa
     5041 atgttttcta agtcgatcgg ggcactaaac gccagcaata actctttaga agcttttccg
     5101 aaaacgccct gcttggtaaa aatcgtcgcg tattctggaa agacttgatc caatacggct
     5161 ataatttttc ttttgaagtc gctcgctgta cctactaaat aagttcggta acgagaaagt
     5221 tgtttcaaag aaaaaacatt ttcatcggat aagaccgttt cgacaaatga gccatagcgc
     5281 attaagtcag caatcaaaac agaatcaata atatcgtttt ttcgtttccg tatttctgta
     5341 cctttacgcc atccgtctgt ttgaagggga ttcacaacgt gaatcaaata atctgattcg
     5401 tgaaggtatg aaaagatgga cagccaatag tgccccgtgg cttccatccc tataacaaag
     5461 tcactgggtg ctccggaata gtcgaccatt cgctcaatta atgactctgc tccttcagtg
     5521 gtgttaggaa atgaaaaacc ttttagtaat acctttcctt cctcgttcat gatagaagca
     5581 acgtgagtgc gtttaccgat atcaatgcct aaaaagtaca tgtatatctg acctttcaaa
     5641 aaaagaattt tggattgaaa tccactcaac tgaaaagcgt cactaccttg tacgagatac
     5701 ggagtaaagt aaggactttc aacatctaac tcatacgcaa tccacttatc agagagagga
     5761 gccagtctct cgagtgcggg ttcaatggcc caagggggga acggcttagc ctctcaatcc
     5821 aataaatata ttatctcaaa agaaatgagt taataccaaa ttaaaataac gatggttcat
     5881 agggttatcc aatcaaaacc ggaaggttaa aaccctaact ctaatataca agggggaata
     5941 atatgcttca aaattttatt aatggtctgt atgactttca ctttttacag aatgctctaa
     6001 ttacatctgt agttataggg attgtttctg gaacagtagg ttgttttatt attttaagag
     6061 gaatgtctct tatgggtgat gctatttctc atgctgtttt gccaggagta gcaatttctt
     6121 atattttagg tattaatttt tttattggtg cgattgtatt tggtctatta gcttctatca
     6181 ttattacttt tataaaaagc aatagtgtaa taaaaggaga tacagcaatt ggtattactt
     6241 tcagttcttt tttagcatta ggagttatcc ttataggagt tgcaaatagc tcaacggatt
     6301 tatttcatat cttatttgga aatattttag ctgtacaaga tattgataag tggattacaa
     6361 ttggcgtttc tgttttagtg ttggttacag ttgttctatt ttttaaagaa ttattaatca
     6421 cttcttttga tccattaatg gcaaaagcaa tggggatgaa tgtcagtttt tatcattatc
     6481 tattaatgat tctattaact ttagtagcag taacagctat gcaaagtgtg ggaactatat
     6541 taattgttgc aatgttaatt acaccagctg ctacagcata tttatatgca aacagcttaa
     6601 aaactatgat atttatttcg gcgacagtcg gagcgtgctc atcattgcta ggtttgttca
     6661 ttggttatag ttttaacata gcagcaggat caagtattgt tttaacagcg gcattcttat
     6721 ttgtaattag ttttctaatc tcaccaaaac aaaaattttt aagaaagaag gagagaaagt
     6781 tatgatgaga aaatggaaag cagtactggg aagtctgggg attttgattg ctctttttat
     6841 attcggggca tgttcaacaa atagtaaaga caaagataca gtggcttcga acgaaaaatt
     6901 aaaggtagta gttactaatt cgattttagc agatattact gaaaatatag caaaagataa
     6961 aattgattta cacagtatcg tacctattgg gaaagatccc cacgaatatg aacctttgcc
     7021 tgaagatgtt caaaaaactt caaaagcaga tttgattttt tataacggtg ttaacttgga
     7081 aactggagga aatgcttggt ttacaaaatt agtaaaaaat gcgaacaaag aggaaaacaa
     7141 agactatttt gcagcaagtg atggcataga tgttatttac ttagagggtc agagtgagaa
     7201 agggaaggaa gatccccatg cttggttaaa tttagaaaac ggtattattt acgctaaaaa
     7261 tattgaaaaa cagttagcgg aaaaagatcc tgataataaa aaattctata aagaaaatct
     7321 agataagtat attgaaaaat tggattctct agacaaagaa gctaaatcta aatttgcttc
     7381 aattccgaat gataaaaaaa tgattgttac aagtgaagga tgctttaaat atttctcgaa
     7441 agcgtataat gtgccttctg cttacatttg ggaaatcaac actgaagaag aaggaacacc
     7501 agatcaaata aaacacttag ttgaaaaatt acgcacaaca aaagttccct ccttattcgt
     7561 agaaagcagt gtggacgata gaccaatgaa aacagtatca aaagatacca atattcctat
     7621 ctattcaacg atttttactg attcaattgc agaaaaagga caagatggtg atagttacta
     7681 tgcgatgatg aaatggaacc tggataaaat tgctgaaggc ctttcgaaat aatttggagg
     7741 gtaggtatgg tacaaattaa aacgactact atactaattt atttactagc aattcaggaa
     7801 ttatcaaaaa gaaaaaagaa attaaaaaat aatgatttag caaaagttct aagtgttagt
     7861 ccagcatctg taagtgagat gctggctaaa cttagatatg aggactattt agatatagga
     7921 tttcaattaa ctgataaagg gaaaaaattt attgacgatt acaaaaaagg ttttaagtaa
     7981 aagactttat ttttagggag gataagaaaa tggctattgg agtgattatg ataggactta
     8041 ccctaatagc tggatataat ttttttaaat ctttctcgga ttattttaga aaaggtgaat
     8101 gatttagatg agtctattaa ttcttgtagg attgcttttt gtatcagtta ttttgctctt
     8161 tctcattagt ttagctctaa ggaatgagtt cagtaataga cgttaacgat tgaaacttca
     8221 agtcctgtgt aagataatat agttaattca tcttattagg aggaagtacc catagaaacc
     8281 agagattata ttacaactca ccaaaaatta atagaaagtg gactaagcca ttttgaaaaa
     8341 gaaggatttc agcaaactaa gataaggcaa ctgtgcttga ccgcaggcgt aactacgggt
     8401 gctttttata aacattttac ttctaaggaa gcgttatttg aagaaattat tcagccgcat
     8461 attaatgcgc ttattaatat gtataatgaa gagtttacca aatacctctt aaacttagaa
     8521 aatatagata tagagaaaag ttggagagag aacttcaatg aattagatcc atttattaat
     8581 tatatttttg agaataaacg tatattcgat ttggttttat tccaatcagc tggaaccaaa
     8641 tatcaagata ttgttgaaaa aattacatca tttgtgacaa aaaagacagt ctgttgtcta
     8701 gataagttaa agactatcgg aatgatcaat caaaatttag tctttaatga agaagaaatc
     8761 cacttctaca tttactcatt ctacgctaca ttctatgata tattgaagca tagctattca
     8821 aaagaaaaga ctatgctatt aaccagaaga ttgtatcaat tttctgttcc tgggtggatt
     8881 gaattactga gttaattaaa aaaaagcttg cattgagaat tgttctcagt tatgcttttt
     8941 gataagatcg cactgttatc tgaataacgg tgcatttttg tcagcttaag ataacagtgc
     9001 gatcttattt tttggaggtg aaagagtgtc aaaaatcaat ccgttaacct tgattacttt
     9061 aaatattttt tttccggtca ttattttttt aggaaaggga aatatatttc aagtggggtg
     9121 tttcattatt gctacagttt tagtattgat gtttcgtaaa ataaagacat gtatttattt
     9181 catcttgatt tattcagttc tgtttttagt aatgaaaatt attgatgcta tcaagataga
     9241 gttttttact atattttttg ggacatcctt atatattgta tttcgaatgt tgccggtcgt
     9301 aatgatctca tggttgttgg ttagtgtcta tactagcaat gaattattat cctctttaga
     9361 aaaaatacat ttaccgaaaa aaataatgtt aagtattaca gtagtgatca gattctttcc
     9421 gacttatcgt tctgaaataa gaatgattaa agaatcttta aaaatgagaa acattcgctt
     9481 gtcttttttt cagcctctgc aatatttgga atttctgtta gttcccgttc ttatacgtgc
     9541 aactatgatt gctgaagaaa tgaccgctaa tgcaataaca aaggggatag aatctcctgt
     9601 gaaaagaagt tcttactata aaacaaacat gagcttatta gactatatca tcctgttttc
     9661 cgttactatt tttttcttaa gtattttgat ttgaggtgaa agaattgttt gaatttaaca
     9721 atgtttcttt ttcatataag gatgttaagg aacaagcgct tgttgacctt aatttttcaa
     9781 taaaaaaagg cagtcttaca ttgattactg gttcttctgg gagtgggaaa tccacactat
     9841 taaagttaat gaatggactt attccaagac tttatgacgg aaaattggtt ggggaaattt
     9901 tatttgaaaa taactcgcta caatccttaa cacaagaaag tatatcaaaa aatattggct
     9961 acgtaagtca aaatcctaga ggacaattct ttacaacaaa tacaacgagt gagttagtat
    10021 tttcgatgga aaactttgga tttacaaaag agcatatgga taaaagaatc aatgaattag
    10081 ctgaattatt agatctttca aatatattag ataaaaacat tttttcgcta tcaagtggtg
    10141 aacggcagaa aatttctatt ggatgtagtt tatcgctaaa ctctaaatta attatattgg
    10201 atgaaccgtc ttcaaatttg gacttcaaag cgactaagaa acttgctgac ttactttgtg
    10261 atttaaaaaa taaaggattt actattgtta tagctgaaca tcgtttttac tatttgaaag
    10321 aattaattga tcaagttcta tttgttaaaa atagaactgt aaaaactaca actattcagt
    10381 tgttaaaaac tgaaaacaat aacgcattaa ggaaatttga catttttaat agttcgattg
    10441 aagttcccta tatcgaaaag gtaagtgcag atgtattaaa agttgaagaa attacttatg
    10501 aaaatattct aaaagacata aattttgatg tcaaagttgg agatgttatt ggtttggtag
    10561 ggaaaaacgg tgtggggaaa acaaccttac taagaatact catgaagatt attcgcccta
    10621 ccaaaggaaa gataatcgaa aataaaaaaa atttgtcatc gccgttttta gtaatgcaag
    10681 aaatggatta ccaatttttc acagaatcag tgaggagcga gttagccctg gggaatgaaa
    10741 tagttagtaa agatcagcaa gaaaaaattt taaaaaaaat ggaactttat aaaatgaaag
    10801 atcaattacc gtttgattta tccggtggag aaaaacaaag attattggtt tctatagctt
    10861 cattgtcaaa aactaattta tttctatttg atgaacctac aagtgggttg gattatatca
    10921 atatggaaag gattgctgac cttattaaag acttaaaaga aaagggtgca gtagttatag
    10981 ccactcatga tccggaattt ctttataaaa catgcaatag aattatttat ttaaaaaatg
    11041 gtggaataaa aaaagatata aaacttagag tagaagattc aactttgata gaacagattt
    11101 ttaatgacat aacgaaatag aattgaggag aatatatatg aaaacaaaag attatatttt
    11161 tattggttta gctacagtta tttccctagt aatttacttt ttttcaataa tggttagtag
    11221 tattggtggg gcgtttggtc acagcatatc cccagggatt tttggattat tatctggaat
    11281 catatttgtt tatattagct ataactaccc tagaaaagga atttttacta tttacacaat
    11341 tgttttattg ttatttttta cgttaatggg cggggcgtac ttaccttggt ttatcagttc
    11401 gattagtatg gcgatcttag ctgatattat tctatctgta tttggatatg atcgagcaat
    11461 tcctcaagtt gtgtcttggg ttctaatgca actaggcagt gctgccggac agtggattcc
    11521 catatggttc tttacagatc gttttcgtca agactggata gatagaggac aatctgctgc
    11581 aacaatggat gcaatgatcc actacgcggt gggtatatgg ggaattattt cagtgttagt
    11641 tgtagcaagt ttatctatga ttggtgtatt gattggaaga aaagtcttaa aaaaatacaa
    11701 aaaatagaac gtagtttacc ttctatctgt ttgtacatct ctaatttacg ctattacgga
    11761 aagcattaac agttgtaaac gaataagaga agaaattgaa tgcgggggag gaaaaaatga
    11821 aagtatacaa aaaattattt agttatgtcc ctaaaatgaa atactacgga tggttagcca
    11881 ctttattttc tgatttttct gtcgtattaa cagtctatgg atattacagt atttacaaat
    11941 ttcttcagtc tctaattatt gtaggagatg aatatttagc taagagttat gcggttcaaa
    12001 ctgttgcttg gttaacttta ggagctttat tttatatttt ttctggctta ttttctcata
    12061 tccttgggtt tcgcttagag actaatttga gaaaaaaggg aattagcggt ttaacagaag
    12121 caagttttcg tttttttgat ttaaattctt cgggatatat tcgaaagaca attgatgaca
    12181 acgcggctaa aacacatatg gcaattgctc acttgattcc agatagtgct caagccatag
    12241 taacacctgt tttatcagtt gccttaggct ttttcattaa ctttaaaatt ggattggttc
    12301 taatcgccat gttagtaatt ggtgtattct tgttaaagaa aatgacagag aatacaaatt
    12361 ttattcaaaa atatcaagaa tcgttagatg tgttaagctc tgaaacggta gaatatatcc
    12421 gcggaattca agttataaag atatttggtg ctaaagtaac atcatttaaa gccttgaata
    12481 aagcaattaa cgattatgct aagtttgcat ataagtattc tttgagtggg aaaacacctt
    12541 ttgttctttt tcaatggcta ttttttggca tagtttgcat tttgatagtt cctataacct
    12601 tttttataac ttctcttacc actcccaaga tcttagctgt tgacttgttg atgttgtttt
    12661 ttttaagtgg aattattttt gtttctttca tgagaataat gtatgtaagt atgcacttgt
    12721 ttgaagcaag atatgcagtc gaaacactag actcccttta tttagaaatg aagcaagacc
    12781 aactaaaata tgggaacgag acacagatta aaaattgtga cattgcattt gaaaatgtca
    12841 gcttttcata caaagaagag tgtgttttag aaaatttcaa cttagtttta gaacaaaaaa
    12901 gaatttatgc attagtagga aaatcaggaa gcggaaagag tacgatagct aaactaattt
    12961 caggtttcta caatgtggat aagggacaga ttaaaattgg tggtaagcct ttaaatcact
    13021 attcaaaaga cacaattatt aacacaattg cttttgtctt tcaagatacg aaactattca
    13081 atcagagtat ttacgataac gtttctatag caaatcctca agcgccagca agtaaagtat
    13141 ttgacgcgct aagattagcc ggttgcgaat cgattttaga aaaatttccc gaaagagagc
    13201 ataccttaat tggtagtaaa ggagtttatc tgtcaggagg agaaaaacaa cgtatagcta
    13261 ttgccagagc aattttaaaa gatgcaaaga ttgttattat ggatgaagcc agtgcatcta
    13321 ttgatccaga taatgagtat gaactacaaa aggcttttca atatttgatg aaagaaaaaa
    13381 cagttattat gatagctcat cgattatcca gcattaaatc tgtagatgaa attcttgtct
    13441 tgtcagaagg aaaaatagta gaacgtggaa atcataatga attaatggct aaacatcgag
    13501 tgtataaaca attggtcact aattatgaga atgcaaatga ttggaggctt gacaatgaaa
    13561 aatttttata aaaaaaaatt tgctttaact gatcagggcg cagaagcttt aactaaagct
    13621 tctatttcta gtttttttgt atactgcatt aatatggtac cagcattcat tataatgatg
    13681 ctgatagacg aattagtttt agaaaatgca aagccccgtt ggctttattt tgcagtatcg
    13741 tttgttactt tgcttttcat gtattggttg ttagataggg agtatgaaaa cttatataat
    13801 agtacttata aagaaagtgc gcatttaaga gtgcaaattg cagacgattt gtcaaattta
    13861 ccattatcct atttttcaaa acataattta tcagatttat ctcaaactat catgtctgac
    13921 gttgaaggta ttgagcatgc gatgagtcat gcaataccta aagccggcgg tatggctctg
    13981 tttttccctt ttatttcagt gatgcttttg gttggtaatg tcaaaatggg attagctgtt
    14041 attttgccaa cgttatttag ttttgtctta atcttgttat caaagaaatc ccaaacgaaa
    14101 gccaatacta aatattacga tactttgaga gaaaactcgg aagaatttca agaaactatt
    14161 gaattgcagc aagagattaa tagctttaat ctatctaaaa aagttcaaga cagacttttc
    14221 aaaaaaatgg aagagagtga aaggattcat ttaaaggtag aattaagtac tttttcagtc
    14281 atggccttat cctctatttt ctcatatgtt agtttagcag tagtcattct agtaggtgtt
    14341 cacttactgt taacgggtga agtgactata ctctacgtcg ttggttactt actagccgca
    14401 ataaaaataa aggattcctt cgattctatg aaagaagcag tgcttgaaat attttattta
    14461 gctcctaaaa tacagcgaat tagagctatg aaggaaacca gtatccaaga gggatcagat
    14521 agtcctctga aatcttttga cattgagttg cgagatgtct ctttctcata tgataacaat
    14581 accccaattt tagatcatat ctcttttacg gctaaacaag gagaagtgac tgcgttagta
    14641 ggtgctagtg gttccgggaa aactagtatc ttgaaattag tgtctagact gtatgattat
    14701 gatgaaggct gtattttaat tgatgggtat gatattaaac gtgtatctcc agcttctttg
    14761 tttagcaaaa tcgctattgt ttttcaagag gtaactttgt ttaatacaag tattttggaa
    14821 aatatacgta ttggaaatag tcaagcgtca gatgaggaag tgaaaaaagc agctcgttta
    14881 gctaactgcg aagactttat tgaaaaacta cctgacggtt atcatacatt ggtcggagaa
    14941 aacggaagtt cactttctgg aggagagcga caacgcctat ccatagcacg ggcttttttg
    15001 aaaaatgcgc caattttaat tttagatgaa attactgcca gtttagatgc ggaaaatgaa
    15061 aaacgtattc aggaaagttt aaatcgttta atacaagata agacagtact aattatatcg
    15121 catcgcttga agtctattga aaaggtaaat aaaattgtag tgatggatca aggaaaacta
    15181 gttgatcaag gtactcataa tgagttgtat cgaaggtcag aaatatacaa aaatctcata
    15241 aagaaaacta aactatctga gaaattcgtc tatgaaaaag aagctcaatc acgatgatga
    15301 aataggcttt aaattaacct acaatttttg ggatatagtt ataattcttt ttcaaaaaga
    15361 ataattttat taaacagcat ctaattttga atacacgaaa gaatcagtat gattgaatta
    15421 gttcacttaa tgtctattct tataatataa ggaataatca aagttttaaa aaattggcga
    15481 taaaccagtt aaacataaaa gattgaataa aaagttatag tttctttcat attgccgttt
    15541 taagtagagg aaaagctact taaaacggta tttgtgtttg attggactaa taaaagtgaa
    15601 ttcttatttt tcagagccta aactgcatat tcttttaaat gatttgatta tctctatgta
    15661 ccgataaatt cgttttcaaa tagttatcta ttcatatgag ttttatttca tgttatttat
    15721 tgttgacaag agcaactctt gttggtaaag tatcagtgtc aaaacgtaac gattacgatt
    15781 tgaggtgtct aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt
    15841 gctaaagcaa agaaacaaat ggcgatgatt gaaaagtatg cagataaacg tcaggaatta
    15901 aaagccgcag gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt
    15961 ctgaggcttc gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg
    16021 tcccgtatta agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca
    16081 agttggtaaa aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga
    16141 aacggttaag aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga
    16201 atggtgatta tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag
    16261 taattaaagg agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa
    16321 attatttacc cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa
    16381 tcgctagata aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg
    16441 ggtttaccga cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa
    16501 ggcttatcat ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag
    16561 ttatttcaat gtatcgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt
    16621 tcacaattgg aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt
    16681 gaagatcgat aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg
    16741 tcgaaactgg agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt
    16801 tagaattaaa aaagtattcg ccaaaattgc gcagacgggc aatttttaaa gaagtgaaat
    16861 agctaagtgg taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt
    16921 aggggctgga aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga
    16981 agaaattatt ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt
    17041 tcatagcgag gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag
    17101 tgatttatta gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat
    17161 ttcacaaatc atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact
    17221 tttaacagcg cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga
    17281 tagtgacagt tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatggc
    17341 agatcgcatt tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca
    17401 gagagaaatc aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac
    17461 gattcaaaaa aaggattttt ataatttgaa aaaatttaat catcccttag tttttgaaag
    17521 tgattcatca gaaaatcact ataatggcaa atactatcat caacaccatc aagtacatga
    17581 gtttaaatca attctcttaa cttcagatca agacataaaa aaagattttt tactccaatg
    17641 gattgactgg ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc
    17701 tttacaagag caggatttta tagttgccat gcaagaggta aacgaacagg ttaattttca
    17761 atacaccact cgacaaaaag accaaaccac tattattcta atcggaaaaa tgttggatga
    17821 agaaaggatc aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtagt
    17881 gattgggggt aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa
    17941 agcccgaata ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat
    18001 aaaatgaaga aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac
    18061 ttattgggat agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag
    18121 tatcgaatga taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt
    18181 tcaccaaaaa catcgtcggc gatgaagcga acgtggattt aatggttcca gcgggatcag
    18241 aacctcatga ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt
    18301 ttgtatatca taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga
    18361 aagctgggcc taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag
    18421 aagaagatca tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg
    18481 tttcacccaa aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt
    18541 atccgaaaaa agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac
    18601 ggttggatgc tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacgc
    18661 aacacgcagc ctttggatat ttagctttgg attatggatt gaaacaagtt cctattgctg
    18721 gactaagtcc tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta
    18781 aaaaaaataa gatcaattat atttactttg aaaagaatgc taatgataaa attgcaaaaa
    18841 ctttggccaa cgaagctggg ataaaattgg aagtactgaa tcctcttgaa agtcttacaa
    18901 aagaacaaat ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt
    18961 tagaaaaaac aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg
    19021 aaaagacagt tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg
    19081 attatacagg cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag
    19141 ttttcgatta caaagcaaaa atcaataaag acatgacagc tgccgagtac aaagattatt
    19201 atactactgg ctataagaca gatattgata ctatcaacat taaagacaac accattgatt
    19261 ttgtagtcaa cggtgaacat catcaataca catacaaata tgttggatac aaaattttga
    19321 attatgaaaa aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg
    19381 gtcgttttaa atatattcaa tttagtgatc atggaattgc accaagtaag gcagagcatt
    19441 tccatatttt ctttggtgga gaaagtcaag aaaaactcta taatgaattg cataattggc
    19501 ctactttcta tccagcttca ttgtctaaac atgaaattgc tcaagaaatg atggctcatt
    19561 aaaattcaat ttttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa
    19621 gaatattgat gcttatcggc gttatcgatt tgcaaagcaa atgagattaa atgggagtac
    19681 attagctgat attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa
    19741 acgggatcct gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa
    19801 acggcataat agcttggcca attaattgat aataccagag tagatcattt ttcccttatt
    19861 tggatttgat ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca
    19921 aagaagactt cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt
    19981 tcaatcatct gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag
    20041 tattctcgaa tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat
    20101 ctggaaaata aaagggcata ttattcaatg cttctgtaac tgcgaaatga ttggagaatt
    20161 cttttcctct atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt
    20221 caatcatttt ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa
    20281 ggaagcatga ttttctgtct gtcaaagtca ctaaacaagt accaccagtt ttgccagcta
    20341 caatatcggc tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggacggccat
    20401 ggatcgaatg agtaaggcaa atctttccac gagactcgac atgatttttt gtatgtcttg
    20461 tttttcctcg atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg
    20521 tatcgaaaag atcattgcaa attgcacgtt agatcgtgat aaaactaatt gaaaagtcca
    20581 tatgttcatg cttcaaacga ttcgagattt cctctggcga ccactgaaaa tcgacaaata
    20641 aatgacgaac aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt
    20701 ttcttcgata agcataattt ttttccgctt tggaaggtga atagtttttt tcttgtttct
    20761 tctcagttca tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat
    20821 agaaaatcct tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag taagatgacg
    20881 atactggctc ataattattt tccttctagg agtgttctcg acaaacttat actagttgga
    20941 tatcgattat gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag
    21001 aggttcccta tgaaaaaaat acctattaca atcttaactg gctatcttgg ttcaggaaaa
    21061 acgatacttc taaatcatat tctgtacggt gatcacggaa aggaaattgc tgtcattgta
    21121 aatgacatag gggaagtaaa tgttgatggg gtattagtcg acaagaaagg ttttaaaaga
    21181 actggagaaa aacttgtttc gatgccgaat ggatgcattt gttgtacatt acgcgaagat
    21241 ttgattgttg aactagatca attagtgcaa ttttctgact tgaatcaaat tgttattgaa
    21301 gcttcaggaa tcagcaaacc aattccaatt attcaagcaa ttatattagc taaaaaccct
    21361 ttagacattg atttgaccca acgattagag attggtgcca ttattatagt agtggatgta
    21421 aaacgttttt gggataattt cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt
    21481 aatgtagttg aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt
    21541 aacatattac ttttaaataa tagtgactta gtgtcaacca agacgattta caaaattaac
    21601 ggtctaattg ctaagttaca gtccaatgcc aaaataatta ggacaattaa aggtgaagta
    21661 gacttatcga aaattctaaa tacaaatctt tttaatatag atgagacttc ctattcagtg
    21721 ggctggttca aagagttaga gcttggctat caaaatcata ttcctgaaac agaagaatat
    21781 ggtatctcaa gttttatcta tcgaaatcgg ttactccttg acgcacaaaa atttgcccat
    21841 cttacggata aaaattttcc tggcaatatt acaagagcaa aagggtttct ttggtttgcc
    21901 caacatagtg atttttcgac gatctttgaa acatcgggta gctataaaag tattgaatca
    21961 ttaaattatt gggtagctag tttacctaaa attgagtgtc aaatgattat acaaaaaaat
    22021 cccgaattga aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt
    22081 attggtatga agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat
    22141 tctcttaatg cagataattt ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg
    22201 tttagccaga tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc
    22261 ataattattg gcattttgat tattacctct tttattccct tatttgtatt ctctatagaa
    22321 aaggagaatg aaacaactga agatgctatc aaatatgcca tcgttcgaat tgatggcaag
    22381 gaaatggatc gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg
    22441 aagggacaat ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat
    22501 agtcctgatc aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt
    22561 tgtttacccc ataagttagt tattagtatt gagaaaaaag aaagcacaga ttactacatt
    22621 tactaatctg gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat
    22681 agcaggggct ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac
    22741 aggcacaagt atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt
    22801 attt
//
LOCUS       DACEJZ010000045        22480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEJZ010000045 DACEJZ010000000
VERSION     DACEJZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.045"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW656_001580"
     CDS             107..970
                     /locus_tag="IW656_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0837292.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW656_001581"
     CDS             complement(1099..2721)
                     /locus_tag="IW656_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0837293.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW656_001582"
     CDS             2970..3491
                     /locus_tag="IW656_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837294.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW656_001583"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW656_001583"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0837295.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW656_001584"
     CDS             5572..6390
                     /locus_tag="IW656_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0837296.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW656_001585"
     CDS             complement(6493..6690)
                     /locus_tag="IW656_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837297.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW656_001586"
     CDS             6859..7437
                     /locus_tag="IW656_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0837298.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IW656_001587"
     CDS             complement(8482..8985)
                     /locus_tag="IW656_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0837299.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IW656_001588"
     CDS             9344..10369
                     /locus_tag="IW656_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0837300.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10716..11594
                     /locus_tag="IW656_001589"
     CDS             10716..11594
                     /locus_tag="IW656_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837301.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12070..12186
                     /locus_tag="IW656_001590"
     CDS             12070..12186
                     /locus_tag="IW656_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0837302.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13114..13452
                     /locus_tag="IW656_001591"
     CDS             13114..13452
                     /locus_tag="IW656_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837303.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13546..13776
                     /locus_tag="IW656_001592"
     CDS             13546..13776
                     /locus_tag="IW656_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837304.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14176..14547
                     /locus_tag="IW656_001593"
     CDS             14176..14547
                     /locus_tag="IW656_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0837305.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14821..15513)
                     /locus_tag="IW656_001594"
     CDS             complement(14821..15513)
                     /locus_tag="IW656_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0837306.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15516..16301)
                     /locus_tag="IW656_001595"
     CDS             complement(15516..16301)
                     /locus_tag="IW656_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0837307.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16538..17938
                     /gene="lacG"
                     /locus_tag="IW656_001596"
     CDS             16538..17938
                     /gene="lacG"
                     /locus_tag="IW656_001596"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0837308.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17963..18280
                     /locus_tag="IW656_001597"
     CDS             17963..18280
                     /locus_tag="IW656_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0837309.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18293..19966
                     /locus_tag="IW656_001598"
     CDS             18293..19966
                     /locus_tag="IW656_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837310.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19980..21611
                     /locus_tag="IW656_001599"
     CDS             19980..21611
                     /locus_tag="IW656_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0837311.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         7036 a         3753 c         4526 g         7165 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tatatttatt ctatcaaaaa attttgttta
    10561 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
    10621 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
    10681 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
    10741 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
    10801 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
    10861 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
    10921 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
    10981 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
    11041 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
    11101 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
    11161 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
    11221 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
    11281 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
    11341 acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa
    11401 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
    11461 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
    11521 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
    11581 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
    11641 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
    11701 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    11761 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
    11821 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
    11881 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
    11941 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
    12001 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
    12061 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
    12121 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
    12181 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
    12241 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
    12301 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
    12361 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
    12421 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
    12481 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
    12541 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
    12601 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc
    12661 atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa
    12721 ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag
    12781 aataacatca aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg
    12841 gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa
    12901 gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt
    12961 gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga
    13021 ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt
    13081 attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa
    13141 catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg
    13201 gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat
    13261 atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta
    13321 gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt
    13381 gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata
    13441 ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa aatttactag
    13501 atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag
    13561 ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg
    13621 aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa
    13681 ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta
    13741 gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt
    13801 tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat
    13861 gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag taagtagcta
    13921 cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag
    13981 cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac
    14041 actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct
    14101 ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
    14161 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
    14221 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct
    14281 tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa
    14341 attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt
    14401 aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa
    14461 acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt
    14521 ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa
    14581 aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt
    14641 aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag
    14701 atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca
    14761 gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt
    14821 ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat
    14881 ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc
    14941 aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc
    15001 ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat
    15061 atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa
    15121 ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt
    15181 ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt
    15241 tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc
    15301 ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat
    15361 ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt
    15421 tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat
    15481 tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg
    15541 gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag
    15601 atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca
    15661 tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg
    15721 atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca
    15781 tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt
    15841 ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca
    15901 tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg
    15961 ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct
    16021 ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga
    16081 gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct
    16141 ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc
    16201 atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg
    16261 accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta
    16321 aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt
    16381 agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta
    16441 tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa
    16501 gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt
    16561 tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa
    16621 aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc
    16681 aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa
    16741 tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa
    16801 tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac
    16861 gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt
    16921 tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt
    16981 caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata
    17041 tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct
    17101 tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc
    17161 gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga
    17221 agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac
    17281 ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa
    17341 tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg
    17401 taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga
    17461 aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg
    17521 aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta
    17581 tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa
    17641 aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat
    17701 ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac
    17761 agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac
    17821 aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta
    17881 cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata
    17941 agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat
    18001 cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca
    18061 aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc
    18121 acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg
    18181 attcattttt atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat
    18241 tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg
    18301 aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta
    18361 tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat
    18421 tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc
    18481 cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc
    18541 aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat
    18601 caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc
    18661 gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt
    18721 cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa
    18781 aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc
    18841 tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc
    18901 atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt
    18961 aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc
    19021 gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca
    19081 attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt
    19141 aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc
    19201 taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt
    19261 caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt
    19321 tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat
    19381 gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg
    19441 aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct
    19501 gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa
    19561 agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt
    19621 ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc
    19681 tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa
    19741 agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt
    19801 tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac
    19861 cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag
    19921 agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa
    19981 tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg
    20041 gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt
    20101 cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag
    20161 atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg
    20221 atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga
    20281 ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca
    20341 gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct
    20401 ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc
    20461 agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct
    20521 ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt
    20581 tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc
    20641 acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc
    20701 gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga
    20761 atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag
    20821 ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg
    20881 aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc
    20941 tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt
    21001 gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc
    21061 tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg
    21121 gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta
    21181 aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt
    21241 ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact
    21301 acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg
    21361 gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata
    21421 ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa
    21481 caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa
    21541 ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg
    21601 tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg
    21661 ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg
    21721 atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta
    21781 tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca
    21841 gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg
    21901 agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt
    21961 ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct
    22021 tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc
    22081 cctccatatt atgttggctc tttattccca ctctgttttc aacgtaaagt gtataaaaca
    22141 gaatgcgata ctatcaaggg agttaaaaca atatacagaa cagtaaaaca ctataaataa
    22201 aaataaaagt gcaataaaag tgcaattttt gcacctaatc atactctgtt tatatatgaa
    22261 aactagattc tgaatccaat atttggttaa cgagaggcgt tcgaatttga acgtctttta
    22321 tatattactt tcaaataaat atgaggtaga atactatcta ttttcttttt aacccaattg
    22381 ttataatgga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc cttctgtaga
    22441 atagagttac cacaacacat tcacagaaga gaggacttcc
//
LOCUS       DACEJZ010000046        21924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEJZ010000046 DACEJZ010000000
VERSION     DACEJZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.046"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            283..522
                     /locus_tag="IW656_001600"
     CDS             283..522
                     /locus_tag="IW656_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837312.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="IW656_001601"
     CDS             complement(884..1144)
                     /locus_tag="IW656_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837313.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="IW656_001602"
     CDS             complement(1329..1826)
                     /locus_tag="IW656_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0837314.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IW656_001603"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="IW656_001603"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0837315.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IW656_001604"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="IW656_001604"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0837316.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="IW656_001605"
     CDS             complement(4548..5210)
                     /locus_tag="IW656_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0837317.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="IW656_001606"
     CDS             complement(5220..6035)
                     /locus_tag="IW656_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0837318.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="IW656_001607"
     CDS             complement(6297..6740)
                     /locus_tag="IW656_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0837319.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="IW656_001608"
     CDS             complement(6874..7212)
                     /locus_tag="IW656_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0837320.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="IW656_001609"
     CDS             complement(7200..7577)
                     /locus_tag="IW656_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0837321.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="IW656_001610"
     CDS             7801..8994
                     /locus_tag="IW656_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0837322.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="IW656_001611"
     CDS             complement(9160..9582)
                     /locus_tag="IW656_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0837323.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9977..10171
                     /locus_tag="IW656_001612"
     CDS             9977..10171
                     /locus_tag="IW656_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837324.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10168..10662
                     /locus_tag="IW656_001613"
     CDS             10168..10662
                     /locus_tag="IW656_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837325.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10824..11996)
                     /locus_tag="IW656_001614"
     CDS             complement(10824..11996)
                     /locus_tag="IW656_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0837326.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12202..13017)
                     /locus_tag="IW656_001615"
     CDS             complement(12202..13017)
                     /locus_tag="IW656_001615"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0837327.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13167..13613)
                     /locus_tag="IW656_001616"
     CDS             complement(13167..13613)
                     /locus_tag="IW656_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0837328.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13610..14614)
                     /locus_tag="IW656_001617"
     CDS             complement(13610..14614)
                     /locus_tag="IW656_001617"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0837329.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14654..14983)
                     /locus_tag="IW656_001618"
     CDS             complement(14654..14983)
                     /locus_tag="IW656_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0837330.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15109..17436)
                     /locus_tag="IW656_001619"
     CDS             complement(15109..17436)
                     /locus_tag="IW656_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0837331.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18279..18572)
                     /locus_tag="IW656_001620"
     CDS             complement(18279..18572)
                     /locus_tag="IW656_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0837332.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18975..20153)
                     /locus_tag="IW656_001621"
     CDS             complement(18975..20153)
                     /locus_tag="IW656_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0837333.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20257..20571)
                     /locus_tag="IW656_001622"
     CDS             complement(20257..20571)
                     /locus_tag="IW656_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837334.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20678..20893
                     /locus_tag="IW656_001623"
     CDS             20678..20893
                     /locus_tag="IW656_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837335.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21193..21435)
                     /locus_tag="IW656_001624"
     CDS             complement(21193..21435)
                     /locus_tag="IW656_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837336.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21422..21850)
                     /locus_tag="IW656_001625"
     CDS             complement(21422..21850)
                     /locus_tag="IW656_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0837337.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6454 a         4314 c         3706 g         7450 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     9781 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca acacgggatt
     9841 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     9901 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa
     9961 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
    10021 tactactcaa agcacaagtt cagatcaggc ttccagcagc agtaccacga tctccgaaag
    10081 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa
    10141 agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct
    10201 gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct
    10261 gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa
    10321 gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg
    10381 caacaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc aatcgcacag
    10441 caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca gcaggcacaa
    10501 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
    10561 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa
    10621 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
    10681 ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat agtaaaccaa
    10741 aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
    10801 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
    10861 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
    10921 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
    10981 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
    11041 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
    11101 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
    11161 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
    11221 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
    11281 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
    11341 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
    11401 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
    11461 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
    11521 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
    11581 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
    11641 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
    11701 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
    11761 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
    11821 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
    11881 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
    11941 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
    12001 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
    12061 ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
    12121 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
    12181 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaaatagat
    12241 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
    12301 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
    12361 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
    12421 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
    12481 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
    12541 ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta atttaacagt
    12601 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
    12661 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
    12721 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
    12781 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
    12841 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
    12901 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
    12961 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
    13021 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
    13081 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
    13141 aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt tggcgacgtt
    13201 tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc atcagattta
    13261 cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc gaagggtgta
    13321 gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat gtcatatacg
    13381 ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt tccgttgttt
    13441 atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt aacattttct
    13501 ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac gaaaggaagc
    13561 agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt cattttattt
    13621 ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct gttgaaagga
    13681 ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt tttggaaagc
    13741 ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc gtttttccat
    13801 tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga tgaagcggat
    13861 cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata tatcctttag
    13921 gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca gaaaatgttc
    13981 catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc caatgattta
    14041 tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga gcatctgcta
    14101 gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt tggatggcaa
    14161 gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt
    14221 tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt ccagcaaacg
    14281 aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt tgtgtgtgaa
    14341 atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct agccataact
    14401 gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc tcaggaattg
    14461 ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc ttattctcta
    14521 ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga tacaagtgga
    14581 tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca aatggtgctg
    14641 atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac
    14701 cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg tctactgttt
    14761 ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt tttctttgtg
    14821 cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag acatcatata
    14881 tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg atatttattc
    14941 cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa
    15001 atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag accagtagga
    15061 taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc atcgctgttc
    15121 acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa agatcagtcc
    15181 taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga gagtcggatt
    15241 gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta cacttccttc
    15301 gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa ctgttagggt
    15361 ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa ccgaaatcga
    15421 gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg cgttagcttt
    15481 atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact tgggatttgc
    15541 tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc cagcattaac
    15601 catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg ccacttgtac
    15661 tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg ggacacctgc
    15721 ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt gcttaatcgt
    15781 atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag ctagtttagc
    15841 tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag gcgcagttac
    15901 ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga tttgatcaat
    15961 actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg catatttaac
    16021 gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga ttgatactgt
    16081 tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg cgtcctttac
    16141 gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt ggttctttgt
    16201 ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat tagtcgtcag
    16261 gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa tcatctgtcc
    16321 aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc
    16381 gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac
    16441 tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg tagtcccgta
    16501 cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg tccgattcgc
    16561 attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc ccttaccatc
    16621 aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg ctaactgtat
    16681 accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg ttgagggtgt
    16741 aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc cagcggtagc
    16801 cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg tggagccaat
    16861 aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg ttacgccttg
    16921 taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc catctttggc
    16981 atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag tggccgcaac
    17041 agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg ctgcttttgc
    17101 tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt
    17161 ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat agggtggact
    17221 atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa cgtttacata
    17281 aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca taatgggatg
    17341 taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt gacatatacg
    17401 ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata aaaaatcatc
    17461 aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga tgtatagaaa
    17521 gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc tttaatacaa
    17581 agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa aaaagataaa
    17641 acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta
    17701 ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag
    17761 agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaactagct
    17821 gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt
    17881 ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg
    17941 tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg
    18001 aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc
    18061 atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa
    18121 ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct
    18181 ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc
    18241 atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc
    18301 tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt
    18361 tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct
    18421 gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat gtatttcttt
    18481 tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc taaaactttc
    18541 atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat tctaagaata
    18601 ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt aagaaagata
    18661 ttatcatagt gctctttttt ttactactac ttataggtag atttattcta atccattatt
    18721 ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac atgctaagaa
    18781 aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat taacgacgga
    18841 tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt ttattacctc
    18901 tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt aataccagtg
    18961 ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt taccttttca
    19021 tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc taagcgatct
    19081 ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg acgagctaaa
    19141 tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac gtgatcattt
    19201 acatactctc gacgataagg aacaccacgt ttactagaat caagatacac aaacaaatat
    19261 gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt
    19321 tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact aactgtctta
    19381 gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt atagaacaga
    19441 atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc gctgactcgg
    19501 cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc atgaacaatg
    19561 ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc aatattattt
    19621 atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctcac agacttcatt
    19681 ggattttcac ttatcaaatt ctttctttga caatatttaa aaatcatatt tagaacttga
    19741 ataagtgatt gtaaactatt tttggcatag ccaacatttg ttccattaac gccacattcc
    19801 tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca aatcagacaa ctttttatca
    19861 ccaatccatc gaacaataat ttttaattgt tcctctcgct tatcataggt tatttccttt
    19921 tgttcattta acgaacggta atattggaac cattgctcac ctacaacact aaacttgtca
    19981 ttgcttgcaa caattttttt cttaagcctt tgttgtttct ccatttccag ataagcgagt
    20041 tgcgcctcac gttttgaatt aaagccacgt cttgttgttc gatatgcttg aaagtaccat
    20101 gtgatcctac cagtagaact gatttgcttc ttgaatcgat ctgttttttc catttattga
    20161 ttcctcctat tttgaaggaa cccaacaagc agaaaccctt tggaaatctt tctatctatg
    20221 tcatcattgt accgcatctt agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa
    20281 tttttctcga tagtcattcg aatcactctt tctcatccat tctaaaacat catcacgaaa
    20341 atagtgtcct cgaatcccgt accaacaggg aggaaactca ccaagtttta ctaaacggtt
    20401 cagtgtacta tcacttatct gaaatagtcg ctttaaatca gtatttgtta ataccagagg
    20461 taaatgactg actgcaagca atttttccgt gtaatcttcc agtttttttt gtaaaattga
    20521 atagaactct atttccagag aattaacatt tgttttaact tccaactcca tcacccttct
    20581 ctctcaaaat caaaacgacc accacggtac cccttaaaat tgtaaaaaaa cactccagta
    20641 ctccatcaaa acggactaga aaagaggaat ttttaatatg aaacaaggaa ttgcagacat
    20701 aaaaataatt aaagaaattc ttgaaaaatc tacggctaat gcaatagcat tcggaacagg
    20761 aatcaacttg agtacagtaa aaaaacttaa aagtggagag agagctgaag aaaaattaaa
    20821 tcttgccgat gctattaaaa tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga
    20881 aatatggaaa tagtagtttt tctgatctat cagctgtttt gccaaagcac ataggaatct
    20941 cacctccccc cagtaatggc gggccgattt ggttacggaa gtatcattat cgtagtcgcg
    21001 attctcatat taaaatttgt cgcttaaaag ctaaccttgt catggcgtaa ctagagaatc
    21061 aaaatctaca tgtactgata gatttgctat tcatttgtca aagagcgcta ggctattttc
    21121 agcctatgga aatcctttat tttgacccga taaaaaactt ccatagaatg aaaaggaaag
    21181 ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc ttctcaatca attttgtttc
    21241 cagttgtcta cgcatatatt catctacata cataaccatt cctttttctt cttcataaac
    21301 tggtttcatg gctaaccgtg aaatataatt ttgatatttt gacagaacga tggacatggc
    21361 aattgaatca ccagaagctg cggctttgat tgtcgttagt ggtatgaatg gtatatatcg
    21421 tttattttgc ttcatatttg acacccaaat actttcctag ctcttccaaa gccatttgtc
    21481 gttggtacca aacactgccg agacttttcc caataatatc gccaatttca cgatcattaa
    21541 gaccgatgaa gaaggacata aaaatgattt cttgtctttg actaggtaat gctgcaatcg
    21601 catttgccaa ctcctcattg gaaataaaga ttttcatacc aaggacaata aaagtaccac
    21661 tactattcaa atttggttcc aaataataga actctgtacc taaccctttt tgttccaata
    21721 tttccattga tatttgccgt tggcgaaatc tagcgtactt cttttgaata tttaacgcct
    21781 cattgcagac tactttctta caaaaactgt caaacgtatg ttgaatgtgg gtttcctctg
    21841 agtcgcccat aatattctcc ccctcaaaaa attcattcaa ctcgttttac ttctatccct
    21901 ttccattatt actacaatct agga
//
LOCUS       DACEJZ010000047        21901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEJZ010000047 DACEJZ010000000
VERSION     DACEJZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.047"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(262..816)
                     /locus_tag="IW656_001626"
     CDS             complement(262..816)
                     /locus_tag="IW656_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0837338.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(942..2870)
                     /locus_tag="IW656_001627"
     CDS             complement(942..2870)
                     /locus_tag="IW656_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0837339.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3176..3892)
                     /locus_tag="IW656_001628"
     CDS             complement(3176..3892)
                     /locus_tag="IW656_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0837340.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3893..5716)
                     /gene="glpO"
                     /locus_tag="IW656_001629"
     CDS             complement(3893..5716)
                     /gene="glpO"
                     /locus_tag="IW656_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0837341.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5730..7226)
                     /gene="glpK"
                     /locus_tag="IW656_001630"
     CDS             complement(5730..7226)
                     /gene="glpK"
                     /locus_tag="IW656_001630"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0837342.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7362..8822)
                     /locus_tag="IW656_001631"
     CDS             complement(7362..8822)
                     /locus_tag="IW656_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0837343.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8812..10107)
                     /locus_tag="IW656_001632"
     CDS             complement(8812..10107)
                     /locus_tag="IW656_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0837344.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10439..11116)
                     /locus_tag="IW656_001633"
     CDS             complement(10439..11116)
                     /locus_tag="IW656_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0837345.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11146..12237)
                     /locus_tag="IW656_001634"
     CDS             complement(11146..12237)
                     /locus_tag="IW656_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0837346.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12338..12772)
                     /locus_tag="IW656_001635"
     CDS             complement(12338..12772)
                     /locus_tag="IW656_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0837347.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13356..14813)
                     /locus_tag="IW656_001636"
     CDS             complement(13356..14813)
                     /locus_tag="IW656_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0837348.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14810..16705)
                     /locus_tag="IW656_001637"
     CDS             complement(14810..16705)
                     /locus_tag="IW656_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837349.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16763..18538)
                     /locus_tag="IW656_001638"
     CDS             complement(16763..18538)
                     /locus_tag="IW656_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0837350.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18588..20084)
                     /locus_tag="IW656_001639"
     CDS             complement(18588..20084)
                     /locus_tag="IW656_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0837351.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20278..21900)
                     /locus_tag="IW656_001640"
     CDS             complement(20278..21900)
                     /locus_tag="IW656_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0837352.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         6191 a         4793 c         3607 g         7310 t
ORIGIN      
        1 cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg
       61 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc
      121 ctaccattta actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt
      181 ttggttggct tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc
      241 gacataggtc acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata
      301 caagacaaaa gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag
      361 cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc
      421 tactttgcca taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc
      481 agctacaaac ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg
      541 tcgataccat gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc
      601 aagcaccttc cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata
      661 atcagacaaa cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac
      721 aaaataaaga taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt
      781 ttgtatttct ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac
      841 tcttttcttt ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt
      901 tttctttaca gccactgata ttatgagcta atttgagata atcacttgct gttttcatcg
      961 tttactcttc tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt
     1021 tttgatcctc gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg
     1081 tctaaatcgt aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga
     1141 cgaggcacgt ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct
     1201 tcataaacta acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag
     1261 agctggcgtt cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct
     1321 tttttctgtg tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc
     1381 atcgacatat ccattttatg agcaatccac aaagcttggc tttctcctgt ttctccaacc
     1441 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
     1501 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
     1561 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
     1621 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
     1681 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
     1741 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
     1801 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
     1861 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
     1921 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
     1981 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
     2041 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
     2101 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
     2161 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
     2221 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
     2281 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
     2341 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
     2401 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
     2461 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
     2521 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
     2581 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
     2641 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
     2701 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
     2761 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
     2821 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
     2881 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
     2941 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
     3001 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
     3061 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
     3121 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
     3181 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
     3241 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
     3301 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
     3361 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
     3421 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
     3481 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
     3541 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
     3601 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
     3661 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
     3721 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
     3781 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
     3841 ggatcagtgt cccaataaac tcccccatga tttgagtcat atcactgttc atttatttct
     3901 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
     3961 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
     4021 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
     4081 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
     4141 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
     4201 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
     4261 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
     4321 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
     4381 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
     4441 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
     4501 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
     4561 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
     4621 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
     4681 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
     4741 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
     4801 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
     4861 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
     4921 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
     4981 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
     5041 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
     5101 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
     5161 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
     5221 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
     5281 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
     5341 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
     5401 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
     5461 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
     5521 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
     5581 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
     5641 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
     5701 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
     5761 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
     5821 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
     5881 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
     5941 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
     6001 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
     6061 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
     6121 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
     6181 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
     6241 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
     6301 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
     6361 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
     6421 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
     6481 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
     6541 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
     6601 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
     6661 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
     6721 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
     6781 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
     6841 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
     6901 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
     6961 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
     7021 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
     7081 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
     7141 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
     7201 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
     7261 taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt
     7321 ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc
     7381 agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga
     7441 taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta
     7501 ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat
     7561 tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt
     7621 tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta
     7681 agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc
     7741 gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca
     7801 cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt
     7861 tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac
     7921 atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa
     7981 tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac
     8041 atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt
     8101 ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt
     8161 gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc
     8221 tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac
     8281 ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg
     8341 aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg
     8401 atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa
     8461 gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat
     8521 aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc
     8581 aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact
     8641 tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct
     8701 tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca
     8761 aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc
     8821 ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa
     8881 aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttttactg caattgtcag
     8941 ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt
     9001 ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta
     9061 gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc
     9121 tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc
     9181 ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt
     9241 tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc
     9301 aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc
     9361 atcattcaat gttaattgtt cttcccacat ctgattgtcg gggcgagaat gattagccag
     9421 caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc
     9481 ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt
     9541 tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc
     9601 gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg
     9661 tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac
     9721 gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt
     9781 ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa
     9841 aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata
     9901 gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc
     9961 ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa
    10021 atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa
    10081 cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg
    10141 ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag
    10201 cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg
    10261 tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct
    10321 tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac
    10381 gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt
    10441 aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc
    10501 ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt
    10561 ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat
    10621 ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc
    10681 cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc
    10741 cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc
    10801 acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat
    10861 caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc
    10921 aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag
    10981 gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt
    11041 cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata
    11101 cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc
    11161 tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg
    11221 tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac
    11281 gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc
    11341 gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac
    11401 cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt
    11461 taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt
    11521 gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat
    11581 gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat
    11641 aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg
    11701 agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc
    11761 tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc
    11821 ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga
    11881 tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg
    11941 aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat
    12001 catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt
    12061 tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc
    12121 atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa
    12181 tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt
    12241 cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc
    12301 gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg
    12361 ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt
    12421 atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt
    12481 ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt
    12541 ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat
    12601 ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca taagttggtg
    12661 ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt
    12721 tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc
    12781 ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat
    12841 gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct
    12901 tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta
    12961 gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    13021 cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
    13081 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
    13141 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
    13201 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
    13261 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
    13321 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
    13381 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
    13441 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
    13501 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atcttcgata
    13561 gcttccttca tctgctcaat atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg
    13621 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
    13681 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
    13741 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
    13801 tggacccctt ccaaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
    13861 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
    13921 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
    13981 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
    14041 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
    14101 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
    14161 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
    14221 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
    14281 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
    14341 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
    14401 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
    14461 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
    14521 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
    14581 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
    14641 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
    14701 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
    14761 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
    14821 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
    14881 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
    14941 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
    15001 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
    15061 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
    15121 cttccccgtt tacaggactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
    15181 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
    15241 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
    15301 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
    15361 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
    15421 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
    15481 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
    15541 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
    15601 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
    15661 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
    15721 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
    15781 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
    15841 gacctacaac taataaagta attgggacca tcacgatgat acaaatgaat ggtgtcaata
    15901 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
    15961 atacagctaa aattgctgga ataaccgtac ttgtatatct tgctagaatc acgggcatcc
    16021 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
    16081 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
    16141 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
    16201 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
    16261 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
    16321 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
    16381 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
    16441 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
    16501 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
    16561 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
    16621 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
    16681 agttcttaga aaacggtttc tccattcaat tagtacccct tctaatagcg aacgaaactg
    16741 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
    16801 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
    16861 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
    16921 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
    16981 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
    17041 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
    17101 aaatctgata aatctccgaa gattcaaact catctagaac actaggtaga taaatcaaat
    17161 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
    17221 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
    17281 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
    17341 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacca
    17401 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
    17461 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
    17521 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
    17581 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
    17641 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
    17701 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
    17761 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
    17821 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
    17881 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
    17941 tcggcatgat attggaaaaa attgtactga tttgttgagg caaccttgcg tgttggatca
    18001 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
    18061 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
    18121 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
    18181 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
    18241 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
    18301 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
    18361 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
    18421 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
    18481 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
    18541 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
    18601 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
    18661 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
    18721 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
    18781 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
    18841 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
    18901 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
    18961 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
    19021 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
    19081 tttttataga cagcgctacg ataataacta aatgcaatat aatctaccgt gttagcttgc
    19141 aagtactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatatcctg
    19201 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
    19261 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
    19321 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
    19381 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatctc tttttttgtt
    19441 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
    19501 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
    19561 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
    19621 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
    19681 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
    19741 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
    19801 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
    19861 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
    19921 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
    19981 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
    20041 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
    20101 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
    20161 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
    20221 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
    20281 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
    20341 tttcagagga taatttgaca caataaccct taaagcttca cgtggaacag tgaacgtctg
    20401 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
    20461 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
    20521 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
    20581 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
    20641 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
    20701 tcctttcgca ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
    20761 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
    20821 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
    20881 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
    20941 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
    21001 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
    21061 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
    21121 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
    21181 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
    21241 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
    21301 cacgtccata cgaaagccac caataccttt atcgatccag aaattcatca tttgataaat
    21361 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
    21421 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
    21481 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
    21541 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
    21601 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
    21661 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
    21721 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
    21781 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
    21841 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
    21901 g
//
LOCUS       DACEJZ010000048        21274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEJZ010000048 DACEJZ010000000
VERSION     DACEJZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.048"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(104..1336)
                     /locus_tag="IW656_001641"
     CDS             complement(104..1336)
                     /locus_tag="IW656_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0837353.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="IW656_001642"
     CDS             1591..2160
                     /locus_tag="IW656_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0837354.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="IW656_001643"
     CDS             complement(2338..2778)
                     /locus_tag="IW656_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0837355.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="IW656_001644"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="IW656_001644"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0837356.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="IW656_001645"
     CDS             3732..4655
                     /locus_tag="IW656_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0837357.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="IW656_001646"
     CDS             4731..5870
                     /locus_tag="IW656_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0837358.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="IW656_001647"
     CDS             5863..6663
                     /locus_tag="IW656_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0837359.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="IW656_001648"
     CDS             6663..7490
                     /locus_tag="IW656_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0837360.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="IW656_001649"
     CDS             7468..8202
                     /locus_tag="IW656_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0837361.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="IW656_001650"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="IW656_001650"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0837362.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="IW656_001651"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="IW656_001651"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0837363.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="IW656_001652"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="IW656_001652"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0837364.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="IW656_001653"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="IW656_001653"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0837365.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="IW656_001654"
     CDS             11787..13820
                     /locus_tag="IW656_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837366.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="IW656_001655"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="IW656_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="IW656_001656"
     CDS             15353..16159
                     /locus_tag="IW656_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837367.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="IW656_001657"
     CDS             16171..17391
                     /locus_tag="IW656_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837368.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="IW656_001658"
     CDS             17381..18967
                     /locus_tag="IW656_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0837369.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="IW656_001659"
     CDS             19006..21144
                     /locus_tag="IW656_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0837370.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6685 a         3615 c         4472 g         6502 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctcc
//
LOCUS       DACEJZ010000049        21127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEJZ010000049 DACEJZ010000000
VERSION     DACEJZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.049"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..895)
                     /locus_tag="IW656_001660"
     CDS             complement(125..895)
                     /locus_tag="IW656_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837371.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(888..1823)
                     /locus_tag="IW656_001661"
     CDS             complement(888..1823)
                     /locus_tag="IW656_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837372.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2417..4066)
                     /locus_tag="IW656_001662"
     CDS             complement(2417..4066)
                     /locus_tag="IW656_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0837373.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(4261..5157)
                     /locus_tag="IW656_001663"
     CDS             complement(4261..5157)
                     /locus_tag="IW656_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0837374.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(5150..5830)
                     /locus_tag="IW656_001664"
     CDS             complement(5150..5830)
                     /locus_tag="IW656_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0837375.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(5831..6613)
                     /locus_tag="IW656_001665"
     CDS             complement(5831..6613)
                     /locus_tag="IW656_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0837376.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(6606..7523)
                     /locus_tag="IW656_001666"
     CDS             complement(6606..7523)
                     /locus_tag="IW656_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0837377.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(7526..7810)
                     /locus_tag="IW656_001667"
     CDS             complement(7526..7810)
                     /locus_tag="IW656_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0837378.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            8598..11825
                     /gene="fss3"
                     /locus_tag="IW656_001668"
     CDS             8598..11825
                     /gene="fss3"
                     /locus_tag="IW656_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0837379.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            11938..12252
                     /locus_tag="IW656_001669"
     CDS             11938..12252
                     /locus_tag="IW656_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0837380.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            12265..12639
                     /locus_tag="IW656_001670"
     CDS             12265..12639
                     /locus_tag="IW656_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0837381.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            12649..12984
                     /locus_tag="IW656_001671"
     CDS             12649..12984
                     /locus_tag="IW656_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0837382.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            13067..14212
                     /locus_tag="IW656_001672"
     CDS             13067..14212
                     /locus_tag="IW656_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0837383.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            14927..16786
                     /gene="ltrA"
                     /locus_tag="IW656_001673"
     CDS             14927..16786
                     /gene="ltrA"
                     /locus_tag="IW656_001673"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0837384.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            16880..17119
                     /locus_tag="IW656_001674"
     CDS             16880..17119
                     /locus_tag="IW656_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ0837385.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            17197..17871
                     /locus_tag="IW656_001675"
     CDS             17197..17871
                     /locus_tag="IW656_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0837386.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            18104..18538
                     /gene="dcm"
                     /locus_tag="IW656_001676"
     CDS             18104..18538
                     /gene="dcm"
                     /locus_tag="IW656_001676"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0837387.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            18539..19246
                     /locus_tag="IW656_001677"
     CDS             18539..19246
                     /locus_tag="IW656_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0837388.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            19236..19526
                     /locus_tag="IW656_001678"
     CDS             19236..19526
                     /locus_tag="IW656_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837389.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            19785..20969
                     /locus_tag="IW656_001679"
     CDS             19785..20969
                     /locus_tag="IW656_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0837390.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            20984..21103
                     /locus_tag="IW656_001680"
     CDS             20984..21103
                     /locus_tag="IW656_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0837391.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         6867 a         3955 c         4223 g         6082 t
ORIGIN      
        1 cactaaacgg tgttcaatca gctgaccctc ggtattcgct caaaaaacaa caaaatatga
       61 ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      121 ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa
      181 caacgatgaa taacacaagt acgatcatcg atgttgctac tggcacatcg atcgttccaa
      241 ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttt gaaacagctt
      301 gccaaaaggg cggtaacata gagatggcat agaaaacgcc acctagataa gttaacggct
      361 gcaagacaaa tgtaggcacg atcgatacat catcataaga ctgagcaaaa atcccgttga
      421 gcagtcctgc taatgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc
      481 atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga
      541 gaatactgcg acccacacca ccaatcacaa agccccaaat aatcacatgc gtaggaacag
      601 gtgctaccaa gatttcttcg atatttttct gaaatttttg agagaagaag gacgaagaaa
      661 cattggcata cgaactagtg atagcagaca tcataatcaa accaggaacg atgaattcca
      721 tatatgaaaa accacccatg tcaccgatcc gcccaccgat catttttccg aaaataacaa
      781 aatacaagga tgtcgtgatc actggcggaa ccagtgtttg gacccatata cgcaaatagc
      841 ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc
      901 tcctccgtat gattattttc ttctgtgatt ttcaagaaca gttcttctaa acgattcgac
      961 ttattcctca tagaaagaac tttgattcct tgtcttgtaa gctgatcgaa gattccgttc
     1021 accccttgat ttcgttcaac ttcgacagcg attgtctgct catcttcaaa tgtacaagca
     1081 tatccttcaa tcacaggttt ggtatcatat ggcgctaaat caaagataaa tgtttcaaat
     1141 tgcagttttg acagaagctc tttcatactt gtattctcga tcagttcacc cgattggatg
     1201 attccgatgt tgcgacaaag catttcagct tcttctagat aatgcgtcgt caaaatgatc
     1261 gttgtaccat tttcgttcaa ttctcgtaga aactcccaca tctcgcgtct caactcgatg
     1321 tcaactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta
     1381 gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt
     1441 tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag
     1501 acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa agggttaaaa
     1561 ttgaactctt gtggaaccaa tccaatctgt tgctttgctc gaactagatc cttgtccaga
     1621 tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt aataatcccg
     1681 atcgttgtag acttcccagc tccatttggc cctaatagcg catagaaatc tccttctttc
     1741 accgaaagat cgattccccg caatgcttcg accccacttc catagatctt tttcaaatta
     1801 tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga ttcttaattg
     1861 tatcatggaa tacagcagat gaaaaaacaa cttggcttcc acacaaaaac acacctgcta
     1921 acaaagagga gatgtgtttt ctagttcatg atcatcttcg atagaataac tatctaaaca
     1981 actctggaac ttttaaaatc ataaaagtac cgacagcaac tactacgatg acatatagaa
     2041 gatccttata actgaggttc ttgtcttttt tattcatgtt aagctttcct ccgtcctttt
     2101 atcgaaagct tttttctttt attttaaccg aaataaataa aatggaaata gttattttca
     2161 atttctgttt tcgctgtttg ttttacggag aactttgcca tggaacgtgc gtctcgtcgc
     2221 tgatgctaag ttttttcgat aaaagctgct agaaggaaga cagcgtggtt tattttgtat
     2281 cgtcttttgc taaatcttcc aagatcatct ggacagcaga ctcgttccca ttgaattctg
     2341 ccactttttt ggcatcaatg tacttctttt gataatcgtc tttttggacc cacgcatcat
     2401 gcttcgtttg ataaagttaa tcaaacggat accactgacg ctgttcatcg aacaacaaac
     2461 tttctttttg ggcctgttca actatggatt taggataatt caaaactttc agcaaggcaa
     2521 tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc
     2581 cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca
     2641 attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga
     2701 ctgaagcgga agaagcaatc cgttcgatcg tatttgttcc ttttaaaatc tcatctacga
     2761 agcagtagca gcgttctttg gaagccactt ggtccagcaa acgtttgatc gatttgattt
     2821 ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca
     2881 catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa
     2941 tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga
     3001 ctaatgtatt cttctgccag ttgactggat taactaccgc ttcttttaac aatggatgat
     3061 agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atcggcatat
     3121 aggtgcgata attcaaaacg gctgctgcaa cttccaattc tcccagcaat tcccacagct
     3181 tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg
     3241 gcagcatgaa catgatgttg acatattcaa aaatcaattc tgcttcactc ccgctcttca
     3301 ttcgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct
     3361 ctttttgaag tggtgtcgtc atttttgcta actgattggc ggctgaaatc gttgaaacca
     3421 aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg
     3481 tattgaatac taagccaccg attgctagat agattcctag tacttgccct aaaagaagca
     3541 aacctacacc caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct
     3601 ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac
     3661 gagcgaattg atattggatt ctttctctca gttgaggatt ttcctgataa aaagcaatta
     3721 gtttttctaa ctgataatct tctccaaaat caaagctacg taaacgctga tacaacgctt
     3781 ctgaacctac actagaatag gtactgttga tcccttcaaa tacttcgaac atatccaagt
     3841 cataccaagt aagatcgtcg atttcactat cccatgaacg gaattttttc tccgtgagcc
     3901 acgcatcctt caaactttct tctttatcaa atctcggttg ataaggaatt tttccccaat
     3961 tactacgaac tgtttccttt actttcaaac gtgaaaaaat gtctaaaaca actaataaaa
     4021 taacaactgc aataataatt aaaacgataa attctctttg gcccatatcc ctctccgttc
     4081 cttgatttgt tagttttagt atagcactgt tgcgctattt gaaaaggggg tacaaaaata
     4141 actgtcttcg ttacctaatt cgtatcaatc acaccaaaat aaaaacacct tccaaaaaag
     4201 aagatgtctt taaaacgaga tacaattttt tgtcattcac ttcaggtaaa ttagagccaa
     4261 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
     4321 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
     4381 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
     4441 ttgcgctcct ggtttgatag gattggccac tgttaatcga atgaatgaat cctcttttga
     4501 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
     4561 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
     4621 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
     4681 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
     4741 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
     4801 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
     4861 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
     4921 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaccc atgtattcag
     4981 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
     5041 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
     5101 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccact taatcaaatc
     5161 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agtttccctc
     5221 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
     5281 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
     5341 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
     5401 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
     5461 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa cagttcttcg atatcaaatg
     5521 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
     5581 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
     5641 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
     5701 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
     5761 gaacctcctc cagtaaagaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
     5821 gcaggaacac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
     5881 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
     5941 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
     6001 ctttgatttt tgcgaaaaca ccgggaacga aggccgttcc catgatataa agaaaagaaa
     6061 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
     6121 tcgctaatat cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
     6181 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaatcatg gctagccccg
     6241 taatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
     6301 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
     6361 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
     6421 ccaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
     6481 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
     6541 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
     6601 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
     6661 ccaactaggt ggataccatc cacaggaact tttgcctcta ccaatgcttg attgatggct
     6721 gctacttggt caattccttc aaaaagctgg ataacacggc tagattggac aaaataatcc
     6781 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
     6841 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
     6901 tcatgcaaaa taccgtagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
     6961 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
     7021 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
     7081 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
     7141 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
     7201 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
     7261 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
     7321 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
     7381 gacttacccg ctccattctc cccaatcaaa ccatagatca ttccacgttt gatttcgatc
     7441 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
     7501 accgtttcat ttccaaaatc catacttatc cttctttctt attgataagt gaagtatagt
     7561 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
     7621 tttcaagaaa aatcagactg tctcgcagac gtggttgagt tgtctgccaa tcaaagagcg
     7681 caccaaacag tgctaatcca agtggactgg ccaccatggt aatcgttgtt atcgttgaca
     7741 tcagacgtcc ttgtatatgc gttggaactt ctcgctgaag ccagaccata taaaaagcaa
     7801 aagaaaacat taatccttaa aaacggactt atgaaaaata atacatgccg tttgggattt
     7861 acaattagct accaaataag aggttatgac aaaagacttt tcacaatccc ttcttccgaa
     7921 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatttatc aaacatgaga
     7981 agcaattttt gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcag
     8041 aagcggataa ataatattgt ttgatgatac tttatctttt tcccgtttgt ttatagatcg
     8101 aatgatatta tagaccaaga atcctaccta ttaacatagc taaaaacaca aaaaaggcac
     8161 tttccaaatt gtggaaagtg ccagaaaagt taggattaag gtaactgcgc tcagcattgc
     8221 ttcgcccagt acgattctcg taactcgtga actcgaacga taatcgcaat taacgttttg
     8281 agaactgtga agctttacgg gctttcttaa gacctggttt tttcttcgta tcattgaata
     8341 agtttgaaaa accttgattt ataaaggttt tcccgtaaat atattttatt atatttgatt
     8401 aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta aaggcagtat atttcgtatt
     8461 ttagattaaa tttttaggct cattcattct caaatccatt ctattcagtc aataaaaatt
     8521 ttaaaaagac tttataaaca aagatgttca agcgagcatc ttattttttt gtgcttaatt
     8581 tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa attatgtgta ttaactattt
     8641 tgagtttggc tgttcaagca attgttggag caattgctcc tacgattact tttgctgatg
     8701 aaattaccca tccacaaacg gtaaccgtag aactagattt agcacatcaa tatgctgttg
     8761 aaggaacttt tagtgatggt cgtccaatgt cagaagtcac tgttccgcat tatgcagtgt
     8821 acaacggctt taaacaagat attttctgta ttgaaccagg agtcccgatt tacaatgagt
     8881 tcactcctgg ctatgagaaa aacccgttac cagatatgtc cgataaagcc aaattagttt
     8941 ccgttttatg gaaaaaagta ggtacagatg cagatactca tattgtggca cagaagatga
     9001 tctggcaaga agttaatggg tatacccttc actcaataaa acgatcagat ggtagtgccg
     9061 taaatatcgc agccattgag gcaaaaataa atcaagccat tgacgattat cagaagaaac
     9121 caaccttcca taatagtacg gctaaaacag tattagggca atcgactact gtgacggata
     9181 cgaataatct gaatttgtca gagttcgatg aggttgtgga aaatgccgca aacattgatt
     9241 atcgtgtgaa tggtaatcag ttgataatca ccccaaatgc caattcaaaa gaaagtggtg
     9301 tgctgacgct taagaaatct gctggtaccg gaacacctgt cgcctataaa aaggctggcc
     9361 aacagacttt aatggccggg gcgattgata aacctaatac ctacacaatc aaaattgatg
     9421 tggaaacgga gggttcttta aagatcaaaa aagtcgataa agaatcgggt gatgttgtac
     9481 caggaacggt tttccattta gactttggaa aaactttacc tgcaaaagac gtgactactg
     9541 acaaagaagg cattgctaca ttggatgaga ttcctcatgg tacaaaagta accattaccg
     9601 aaaaatcagt accggcgcct tatacgattg ataccacccc catgactacc accattaaag
     9661 caggcgaaac catttacgtg acttcaaaaa acgcacggga aaaagggcaa atcattctgg
     9721 ataaaagcgg agttgaaaca gggagtgatc tttggaatga caattattcc ttagctggta
     9781 atacgtttgc cattcgcaaa gacagtccaa ccggtgaaat cgttcaagaa atgaccacgg
     9841 atgaaaacgg tcatgcggaa acaccaaaag agattgctaa tgcgttggaa ttgggaacct
     9901 attatgtgac cgaaactaaa gctagtcatg ggttcgtgaa tacgttcaaa cctgtaaaag
     9961 tcgaattgaa gtatgccaat caaaccgtag cccttgttac cagtaacgta aaagggcaaa
    10021 atcaagaagt cactggcgaa accactttga cgaaagaaga caaagatact ggggataaag
    10081 cacaaggtaa ggcagtcttt gagggtgctg aatacacctt gtttactgct aaggatggaa
    10141 aagctgttaa atggagtgag gcttttaaac cagagttagc gaagggaacg aaaggttctg
    10201 atgaaacggt cactttgact ttagacgaaa agaaccaagt tgccgttaaa catttagcta
    10261 tcaacgagta ttattggcaa gaaacaaaag cgcccgaagg atatagcctt gatgaaacaa
    10321 agtatcctgt atccatcaaa aaggtggatg atagcgaaaa aaatgccgtg attacccgaa
    10381 atgttacggc aaaagaacaa gtgattcgct ttggctttga tttctttaaa tttgctggat
    10441 cagctgcggg cactaccgaa actggcttca atgatctgac ctttaaagtg tcgccattgg
    10501 aagggaccaa tgaaatcaca ggtgccgaag atgaagcgac cacagcttat aacgagcaat
    10561 tagggtttga tggttatggc aagtttgaaa atcttcctta tggggattat ttacttgaag
    10621 aagtagaagc cccagaaggt tttcaaaaga ttacgccatt agaaatccgt tctactttta
    10681 aggaaaacaa agaagacttt gtgaagagtg agtatgtctt taccattacc gaacaagacc
    10741 aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa gctgacgaac aaagcatttt
    10801 ctgttagttt gaaccgttta atgctctatg atttgcctga ggaagaagat agtttaactt
    10861 ctcttgcgac ttggaaagac ggaaacaaag aattaacttc tctggattct accgagctag
    10921 ttgataaatt gagttacaat ctacatgaaa tcaaagaaga ctggtatgtg gtagcgcaag
    10981 ccattgatgt ggatgcgaca aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg
    11041 tgattgcaga aacaagcgca acgttggcga acaaagagaa aacaggaact tggaaaattc
    11101 agcataaatt aaccgccgaa caagttttaa ataaaactat tgtcttattc aactatgtgt
    11161 atgaaaataa ggaagctttt gaagcaggcg ataagccagt agcgaaggat gttagcttga
    11221 ataaccaagc ccaaactgtc agttgtacag tggaacacca tgtttctatc caaacaaagg
    11281 cccacctaga aaacggttct cagaccttta ctcatggtga tgtggtggat atgtttgatg
    11341 atgtgtcgat tactcatgat gtactggatg gctcaaaaga agctttcgaa acaattctgt
    11401 atgctttatt accggatggt acaaacaaag aaatttggaa atctggcaag attgattatg
    11461 aagtaaatga taaagaattc accaaaactg tgcttgcgaa aaaagtagat accggaaaat
    11521 atccagaagg aaccaagttt acttttgcgg aaatcaatta cgataaagat gggaatatca
    11581 atggaaaaca caatgaagat ttgaaagaaa aatctcaaac cttaacacca aaagaagttc
    11641 caaccatact aagtacgccg aaacaaccgg aaacaccaac tgttccaagt ggctctcaag
    11701 aatctagtcc cacagtgaag accttccctc aaaccgggga gaaaaattcc aatgttttac
    11761 tgttcattgg ttttactttg atttttgcga cggcgggcta ttatttctgg aatcgccgga
    11821 actaaggtga tgagatgata caagtaaatc ctaagaacca acgtgcgcga ccgcctccta
    11881 aaaaagtagt cgcgctattt ttgtatccaa aactaacaat gaaaagagga aaagaagatg
    11941 gaattgaaat ttgtcgtgcc agatatggct gaaacctttg gaaagttaag ttatgccggt
    12001 gaaggagaaa ttataacaga aggatatgga cggaatacta cggtgattgg tcgcagttat
    12061 catttgtatt ccagtaagca acgggccgat gatattgagg tggtagtagc tgcggaagcc
    12121 ggtgaaaagg attttgatca agaccaaccg ctaaaagccg tgaatcccca tttggttgcc
    12181 aaaggctatg agattgagaa tcgtgggttt actgattacg tgttgtatgt tgatgattta
    12241 gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag gtattgtcgt agattcaggt
    12301 ttaacatttg ggaagttgcg tttttccgca ttacgccgag aagtacggaa gcaaaatgag
    12361 gatggaacaa ttggtaatga ggtaaaagaa cgaacctata atttaaaatc ctctgcacaa
    12421 gggcgaatga ttcaagttag tatcccagct aatgtaccct tacgcgagtt tgcttatgat
    12481 gcagaggtag agttggtcaa tccaatcgtg gataccgttg ccaattatgt ttttcgagaa
    12541 ggaactaccg ttaattggtt cattaaggcg gatgatttag tcttgaagcg gcagccgaat
    12601 caaggaaatc ctacgaacca aaatgaaaga aagaaatagg tgaagattat ggaaattaat
    12661 gttgaacata ttttagattg tctcgatcaa tatggaaaag gcgagctgac tgaggaacaa
    12721 ctcgttaaag cattaacctc tgatgaaaaa atgtttctga ttatacatca aggtttactt
    12781 ggagaaaata gcgacagtga agaagactct gatgtactag actggttagg agaagaagag
    12841 tctttcttta tagttgtcga agtaaatgaa aacttatgtc ggcaggcaga atccattttg
    12901 gaagaaattg gtgtggagat gccggatgct attgaaggct ttttaaatca attggttgaa
    12961 acgaaacaac tcccctcctc gtagcagtaa atgactagca taactttttg aaattgccta
    13021 atgaaatcct attgggcttt ttcgattgga ggtggaaacc atcaaaatgt acaaaggaca
    13081 tcgcatacgc gcaggagatc agcacttggt ttatcacttt gttcttggct ggctactcgc
    13141 tctgttcatc ggttggatga gcgtctttta ttttcaagag tttagacaat ttgatatttc
    13201 taagctatca ctttctacta ttgaaatcgt ttggtctata aaagatttag tatgtctgct
    13261 tggaagtctc gctttttcag gagctatgat attgctttat attcactttt ttcttgatca
    13321 ttggcgaagc ttgtggcatc gacaaaagct agctcggatg attctagaga accattggta
    13381 tgaagtgaaa caaactcaaa gtgaaggctt tttcaaagat ttgaatagta gccgaaccag
    13441 agagactatc agttacttcc ccaaaatcta ttatcgaatg aaagatggat tactgtctat
    13501 tcgcgttcaa atttcacttg ggaaatacca agatcagctt ttaaaattgg aaaagaagtt
    13561 agaaagcgga ctgtattgtg agttagtaga aaaggaactc aaagactcct atgtggagta
    13621 tactttactg tatgatatga tcgccaatcg aattgggata gacgaagtag tggcagaaaa
    13681 tgggactctg cgattgatga aaaatcaagt atgggcttat gattctttac cccatatgtt
    13741 aatcgctggt ggcacgggtg gagggaagac ctatttcctc ctcaccatta tcgaagcact
    13801 attgaagtca gatgcagaat tgtttatcct cgatcccaaa aatgcagatt tagctgattt
    13861 aggtacggtg atgcctcatg tttattcaca aaaggaagaa atttctgctt gtgtggaaga
    13921 tttctatgaa cgtatgatgg ctcgtagcaa ggcaatgaaa gaaatgtcta actacaaaac
    13981 aggagagaat tatgcctatc ttggacttcc acccaacttt ttaatctttg atgaatacgt
    14041 ggcttatatg gagatgttaa cgacgaaaga aagtgcggtg attttgaata agctcaaaca
    14101 aatcgtgatg ttgggtcgtc aatctggttt ctttctgatt ttggcttgcc aaaggccgga
    14161 tgcgaaatat ttaggagacg gaatccgcga tcaatgtgcg acatgtttct aactgaaaag
    14221 gttaggcact taaaagaaga aaacataagt ttccttttaa ggagaactga tattttgagc
    14281 agtggaatga tggggtaacg ccctgaaacg ctcccttaat actccgactg gctgtttaac
    14341 atatagctgt tagatggtca gaagctcggt gaagtcggct gaaagttacc gtaaatatga
    14401 taaattcata tacgaaagtt gttcagaggt gaatgatgta acctataggt cggaggtcta
    14461 taaaatactc acggtgagaa tgtaagtaat actactgacg aacttgcgaa tgaaagaatc
    14521 tatacacgga ctgtatagaa atgtacagga ttctaccgta tcaacggaac agaatgtatg
    14581 ttgtggttta gtaagattat tggttatgaa caaccatgca atgttacagg cattggaaat
    14641 atacaggttc aaagcaagca cctaaaagta tatataaaga taaaaatatc gaaacatggg
    14701 aagctggcaa tgtggaggaa actatattcc attgaaacat ttgtgacgaa gaataataag
    14761 cacatttatg ccagtgaaag tggtggcaca gtaccaatga ggatatagaa atatatcgga
    14821 ggaatagcca ctagtcaaaa gaaatcgtta attaaatgat gtatttcata gaatcgagta
    14881 agacaaaata aacactacaa cctaagtaaa caaaggtagt ggtgctatga ataaagtaaa
    14941 aacaaataaa acacttcgtc atagtgaata ctatggcatg acacaaatat ttgatgaact
    15001 gtatgtaaaa agtgataaaa aagaagtttt tacagagctc atgacaatta ttatgagtga
    15061 tgaaaatatt cgactagcat acagaaatat caagaataac aagggcagtt atacagcgag
    15121 taccgataag aaaagtattc gagacattaa aacactgtca gaagatacct ttctaactga
    15181 ggttaaagtt ctttttcgct gttacaaacc acgagcagtt agacgtgtag aaattcccaa
    15241 accaaatgga aaaacacgcc ctctgggcat tccatcaatc tgggataggt taatacaaca
    15301 gtgtatttta caagtcttag aaccgatatg cgaagctaaa tttcacaatt atagttatgg
    15361 atttcgacca aaccgttcca cacatcatgc gattgctacc tgcaatcgac tggcaaatag
    15421 ggggaagcta cactttgtag tagatgttga tattaaagga ttctttgata atgtcaatca
    15481 ctctaaactt ttgcgtcaaa tgtggacgat tggaatccgt gataaatggc tattaggtat
    15541 tattcgatcc atgctaaatg ctcctatagt cctaccaacc ggtaaagtcg aacaccctaa
    15601 aaaaggtgta cctcaaggtg gtgtcctatc ccctttacta tctaatatca tattgaatga
    15661 attagattgg tgggttgctt cacaatggca aaataccacg attgagacag aaaagattat
    15721 ggataggcga gataaaggca aaggcattga taagactaat cagtatcggg cattaagacg
    15781 aacaaactta aaagaaatat acattgttcg gtatgctgac gacttcaaaa tcttttgtag
    15841 aaaacgaagt catgcagata aaatatttca cgcaacgaaa atgtggctta aagaacggtt
    15901 atcacttgaa attagcgaag aaaaatccaa aattgtcaat ctcaaaaaag gtaaatcaga
    15961 atatctagga tttgaattaa agctaatcaa gaaaggtaaa aaatacgtag tcgaaagtca
    16021 tatgagcaaa aaatcaatga caagagttaa aatccaatta aagaaacaat tacgtaaagt
    16081 tgccagaccg aagaatcatt gcgaacaagc aaaagaaatt ggattatata attctatggt
    16141 gattggaatc catcaatatt atggaattgc aacctgtgta aatctcgatt gcagtaaaat
    16201 agcctataca ctaaaaccgt tcatcaatca caaactgccg acaaagaaac atggaaagat
    16261 attaaataca tttatcaaat cgaagtatgg aaaaagtaaa gaaatgcgat ggttgaataa
    16321 tgtgccaatt gtaccgctca actatgttca gtttagactt gctgtccccc tatcagaggg
    16381 aacttgcaag tacactgaaa gtggacgaag caagatacac agtaaattaa aattagacct
    16441 agccctttta cttcacatga tgagaaatag taattatgaa agaagtattg aatttgtgga
    16501 taacagaata tctaaatatt ctgctcaaaa gggaaaatgt gctatcacgg gtaaatttct
    16561 cgaatacgaa gaaatccatt gccaccatat cgtaccagta aaacaacagg gtacagacaa
    16621 atacagcaac ctcattatta ttcacaaaaa tgttcacgca ttagtccatg ctatcgaaga
    16681 aaaaatcata cacaaatacc tgaatgtatt aaatctaacc aatgaacaaa tagaaaaact
    16741 aaatgaatta cgtgtcaaag ctggtaatag tgttttaaca gtttaacttg aaaaatatat
    16801 catacttaac attcttttga tggaacgccg tatgaagtga aagtttcatg tacggtgtga
    16861 agcgggggaa aatctggaga tgacatcaaa ggattaccta tcgctattca actttcgcgt
    16921 ggctttgggt cgtatgagtg aactcggata ttccatgatg tttggggagg tcgaaaaaaa
    16981 cttctttatg aagcacatca aaggtcgagg ttatgtggat acaggtggca gtgtgattag
    17041 tgagttttat acgccacttg taccaaaagg gtatgatttc ttagattcaa ttagtaaatt
    17101 agaaaagatg aatgattgag tgatcttcaa tactgacttt atacttcaag taacgtataa
    17161 tacttttata tagtaggaag aaaggggata attaatatgg aaagtaatga ttcttattta
    17221 gagtatgcaa aagcaaataa ggataagttt atttcttata ttatcaaagg gaaatccccc
    17281 gaagttggac gagacgcagt ttttatggca gggagtcctg gggctggaaa atcagaggtt
    17341 gccattggat tagctgagaa ttatgataac catgtgatta ttgatgcgga ttactttcgc
    17401 acccagtttc ctgattataa tggaaaaaac tctagtctct ttcaaaaagc atcttcttgg
    17461 ttggtagagc aggcatttag atatgtctta gatcatggct attcttttat tctggatgca
    17521 acttttgcaa tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa acattttcga
    17581 gtgacgattt tctatgttta tcaagaccct aaaatcgctt ggaaatttac acaagaacgt
    17641 gaaattgcag agggacgtca tgtgccgaaa aaggcattta ttaacgcttt tttcaaatca
    17701 agagagaaca ttgcaaaagt aaagagtaaa catccagatg tattgcttca tatcataata
    17761 aaagattata cgaataatat ttccgaagtt cattatgcta cggataatct agaattattt
    17821 ttaccactag ggtatagtag gaaagatttg gaggttcaat tacatgactg agaaagaaca
    17881 agtaacgaaa attgtaaaga aatacaataa gagcattgcg gatttatcgg aaaatgctac
    17941 tgcaaaagag ttcaaaacgg tgattaaata tgtagccgat caggccaatg aaaaacaacg
    18001 gaaattagtt ggattaaata aaaaatagta ggacaaatta ataatgtatg agccaagagc
    18061 aaaatgttct tggctttttt ctttgcaatg aatgaggtaa tgtatgagat ttttagattt
    18121 attcgccggt attggtggct ttcgtttagg tatggaacag gcgagccatc attgtatcgg
    18181 tttttgtgaa attgataaat ttgctagacg tagttataaa gcaattcatg atacaagtaa
    18241 ggaggtggaa atgcatgaca tcacaagtgt atcagatgag tttattcaat ccctcggacc
    18301 agtggacatc ctttgcggcg gatttccgtg ccaagctttt tcaattgcgg gaaagcggca
    18361 aagattttcc gatactcgag gtactttatt ctttgaaatc gctcggttca ccactcttct
    18421 ccgacctaag cttttattcc ttgagaacgt ccgaggattg ctcaatcacg aaggaggggc
    18481 tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgacgtgg agtggtaggt
    18541 gcttaactca aaggcctaca ttccccaaag ccgggagcgg atattcctta tcggacattc
    18601 tcgagacgca tgtaccgaac aagtatttcc tatcattgga ccgtcttcaa catctgatca
    18661 aaacatcaga aatttggtaa atatcaatcc ttccaatcga ggaatgggtg gacaagtcta
    18721 tggttcagat ggcgtagcgc caaccttaac aggtgatgaa ggaatcaaaa tcgcactgcc
    18781 agcaagggat ggaatctctg tagcaggact attaccggga aagtttgaac aaggtaatcg
    18841 agtttatgaa gttactggaa ccgcccccac cttgtcaacg aaacaaggtg gtacaaaaat
    18901 catgattcga aaaaatgaaa cagaatatca agaggtgaag ccagaagaca gtgtgaatct
    18961 ggcttttcct aattccacaa gtcgccgagg tcgtctggga aaacagagtg ttcatacctt
    19021 actaacagga gaccaacaag cgatcgtcac ggatcagtat caaattcgga agctaacacc
    19081 tagagagtgt tggcgacttc agggctttcc cgattgggct tttgatcgag attctcaagt
    19141 caattcagat agtcagttgt ataagcaagc cggaaactct gtaacagttc cggtcatttt
    19201 tgatattgcc agaagattaa aagagttgga tgtacatggt ttatgatgag ttgattggag
    19261 aaatttattg ggtgattgga aagattcagt cagacccaga attagaagaa caattgcgac
    19321 ggttaaaatt tgatattcgc aagaatggtg tcaaagtcgc tggcgatcca tatttaatga
    19381 atgaggagac ggatgctcga attgaaataa atcaagtaat tgcagaattt gagcggattg
    19441 cggaccaagc aaaagaacca gatattcgtc aatacttgtt agaaatgaaa gcagagttgg
    19501 aagtaaatgg tattacagat gagtaaccta aaccaacaca actgactgtt aggcgctgac
    19561 cgcggaggat tcgcgaagca gaaatcctcc gtggtgctgt gcctgttcat ggacggcgtt
    19621 agccgaccat gatgtctcac ccccgtatct aacagggggg tacaaaaaca caaaaaataa
    19681 gtaagcaacc attaagaatg cttgttgtat caaagatagt aagtaatttg tataggtgtt
    19741 aactcatttg aaaaagttaa caccttgaat ttggaggtga gaacttggca caacggaatt
    19801 tagattatcg gctattaaaa gaccgacgca acgaatacgg cgtttcacaa aataaattgg
    19861 ctacggcttg tggacttagt cggccgtact taaatcagat agaaaatggt ggcgtaaccg
    19921 catctataaa aaccatgagg aaaatttttg accaactgga aagctttaat cccgatttgc
    19981 ctttgacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat gcacgaaaaa
    20041 ttattcaaga aattctccat ttgaaatttg agtatatgct ccatgaagat tatgcctttt
    20101 actcctatca agaacagtat gtcatgggtg atattgtggt gatgttgtct catgaagaag
    20161 ataaaggtgt gcttttagaa ttaaaggggc gaggttgccg acaatttgaa acttttttac
    20221 tcgctcaaaa gcgtagctgg tacgactttt tcgaggattg tctaaaggcc ggtggcgtga
    20281 tgaaacgctt ggacttggca atcaatgatc gagtggggct attggatata cctgagctaa
    20341 cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg tacctttaaa agttatcgtt
    20401 ccggggaact tttaaaggct gatgaaaagg acggcatggg caatacctta tatatcggca
    20461 gtctcaagag tgaggtttat ttctgtttgt atgaaaaaga ctatgaacaa tatatcaagt
    20521 tagggattcc attagaccaa actgagacga aaaatcgttt tgagattcga ctgaaaaatg
    20581 accgtgcttt tcatgcgatt caagatttgt taaaaggtcg tagcattgag agcaccacgt
    20641 tttccattat caatcgttac ttgcgatttg cggataaagt agaaggtaaa aggcgaacca
    20701 actggccctt gaatgaacaa tgggggcgtt ttattggacg gaatcggaaa gagattcaat
    20761 taacttccga gccaaaaccg tataccattg aacgaacctt gaattggcta ggccgacaag
    20821 tcgcccctac gtggaaaatg gctaaggagt tggatcgatt gaatcggaca acctatatcc
    20881 aggatatggt acagaatgca cgcttatcag accgacataa aaagattttg gagcaacaaa
    20941 gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtcatgggaa ttgtaaaaga
    21001 tatttttttg gtatttggtg gcagtatgct tggtgttact gtgatgtgct tgatgaatgc
    21061 aagtgcagca gcggatcgag caatggaaaa gaaaggaaag tgacaagatg aatttcggac
    21121 aaaattt
//
LOCUS       DACEJZ010000050        21030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEJZ010000050 DACEJZ010000000
VERSION     DACEJZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.050"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..529
                     /locus_tag="IW656_001681"
     CDS             <1..529
                     /locus_tag="IW656_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0837392.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPV
                     TFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGV
                     LLVFISIKFRKKRNI"
     gene            complement(953..1123)
                     /locus_tag="IW656_001682"
     CDS             complement(953..1123)
                     /locus_tag="IW656_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0837393.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1317..1496)
                     /locus_tag="IW656_001683"
     CDS             complement(1317..1496)
                     /locus_tag="IW656_001683"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837394.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1883..2995
                     /locus_tag="IW656_001684"
     CDS             1883..2995
                     /locus_tag="IW656_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0837395.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3073..3366)
                     /locus_tag="IW656_001685"
     CDS             complement(3073..3366)
                     /locus_tag="IW656_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0837396.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3534..3848
                     /locus_tag="IW656_001686"
     CDS             3534..3848
                     /locus_tag="IW656_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0837397.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3869..5848
                     /locus_tag="IW656_001687"
     CDS             3869..5848
                     /locus_tag="IW656_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0837398.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5861..6187
                     /locus_tag="IW656_001688"
     CDS             5861..6187
                     /locus_tag="IW656_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0837399.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6203..6721
                     /locus_tag="IW656_001689"
     CDS             6203..6721
                     /locus_tag="IW656_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837400.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6721..8073
                     /gene="celB"
                     /locus_tag="IW656_001690"
     CDS             6721..8073
                     /gene="celB"
                     /locus_tag="IW656_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0837401.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8157..9662)
                     /locus_tag="IW656_001691"
     CDS             complement(8157..9662)
                     /locus_tag="IW656_001691"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0837402.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9754..10344)
                     /locus_tag="IW656_001692"
     CDS             complement(9754..10344)
                     /locus_tag="IW656_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0837403.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10523..12169
                     /locus_tag="IW656_001693"
     CDS             10523..12169
                     /locus_tag="IW656_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0837404.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12218..12937
                     /locus_tag="IW656_001694"
     CDS             12218..12937
                     /locus_tag="IW656_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0837405.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13416..14363
                     /locus_tag="IW656_001695"
     CDS             13416..14363
                     /locus_tag="IW656_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0837406.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14375..15295
                     /locus_tag="IW656_001696"
     CDS             14375..15295
                     /locus_tag="IW656_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0837407.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15336..16793
                     /locus_tag="IW656_001697"
     CDS             15336..16793
                     /locus_tag="IW656_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0837408.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17138..17752
                     /locus_tag="IW656_001698"
     CDS             17138..17752
                     /locus_tag="IW656_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0837409.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17745..19469
                     /locus_tag="IW656_001699"
     CDS             17745..19469
                     /locus_tag="IW656_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0837410.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19466..20917
                     /locus_tag="IW656_001700"
     CDS             19466..20917
                     /locus_tag="IW656_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0837411.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6780 a         3553 c         4236 g         6461 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag atggcttagc
      241 accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat tggatgcgac
      301 accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa caaaagagaa
      361 tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca gtggaaataa
      421 gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa tattgggtgt
      481 gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag ctaaaataaa
      541 aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc
      601 gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt tgaacccaac
      661 cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa acctttgtag
      721 taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg agtttcgcag
      781 tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt tctacaaata
      841 atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa taaggagaaa
      901 aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa ggttacgcct
      961 ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca tttggtagaa
     1021 catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat tctatttctt
     1081 taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt gtaggtaaat
     1141 tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt tatcctcaac
     1201 ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct tttggaagac
     1261 tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa agatacctaa
     1321 caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt gattttatgt
     1381 ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc gttccaatgg
     1441 cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat tggcatatga
     1501 ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac cattaatttc
     1561 ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc agggaagaga
     1621 actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc
     1681 atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat aaagcatatc
     1741 gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat atgttttttt
     1801 ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata tgatgttcca
     1861 tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga aaggacgaca
     1921 acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac aaaaaatacg
     1981 agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt tacgatcatt
     2041 attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat ttgaatcgaa
     2101 gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt ttgttgactt
     2161 tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct cactattagt
     2221 agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc attttatttt
     2281 actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg cttctttgta
     2341 cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga acaagaattt
     2401 ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag ttctccttta
     2461 cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt ttttgaaagg
     2521 catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta gtcttttgat
     2581 cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg cattttcttc
     2641 tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat
     2701 cattggctca ttgattggat gtgttttatt tattggtatc agccaattta ttccttttaa
     2761 ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata gatataaaac
     2821 agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg ttccaggagc
     2881 agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca tgtatattgg
     2941 ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc attagatgag
     3001 atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg ctatcattcc
     3061 atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta atttatactt
     3121 tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt cgattgcttc
     3181 ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg gaaagtaata
     3241 gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc tttcgaccat
     3301 ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat tcactgtgat
     3361 actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg ccagaaatta
     3421 ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata ttttgaatag
     3481 aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga tttatgaaaa
     3541 aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca aagacaacgg
     3601 atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct acagaaggcg
     3661 ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa acgacgatgt
     3721 ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt agtatccctt
     3781 tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg atcgaagaga
     3841 acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc tgttgagata
     3901 tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg aattgttatt
     3961 atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag aaaaaaacgg
     4021 aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac aaagactgac
     4081 atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag aaccgcaagg
     4141 attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt tggaagatgc
     4201 cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca gtttgacaaa
     4261 ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg tttctaagca
     4321 tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta tcatggatac
     4381 tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa gccgtttttt
     4441 cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa gagaagctaa
     4501 gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt tggggatcaa
     4561 acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag tttctaagcg
     4621 agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc
     4681 gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt cgaatcacaa
     4741 agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga aaagattgtc
     4801 tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac tactcgatca
     4861 tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc cattgactga
     4921 ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat
     4981 gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg ctttgcatct
     5041 catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga ttatctgtgc
     5101 aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa
     5161 tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt taaaaggaat
     5221 cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc cagttttaca
     5281 tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc
     5341 tataccctat cattcctcag atagaccgat accagcattt tctctacagc aaaaaaagca
     5401 gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa
     5461 ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag aaggatatat
     5521 aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca tattcagtga
     5581 tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg tagccgttgc
     5641 attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg tttttttagt
     5701 ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt
     5761 agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg agaatttcag
     5821 cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa aaataaacag
     5881 tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg cacggacact
     5941 gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag aagagaagtt
     6001 gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac ttctgaaaga
     6061 atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac aagatcatct
     6121 gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt atcaaaaaaa
     6181 taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa
     6241 aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac gttacctgac
     6301 tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag ctaaaagttt
     6361 aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag gagaacaagt
     6421 aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt ttttgttcca
     6481 gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta cattcttgta
     6541 tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg ttgtaagcgg
     6601 tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca accaagacca
     6661 acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg agaataacta
     6721 atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc gcaacttaaa
     6781 atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat tgtaggctcg
     6841 atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg attttttaat
     6901 gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac gatggggatt
     6961 ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga acgagatgaa
     7021 caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc attctttatg
     7081 tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc tatcaatgag
     7141 acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt aggaacttca
     7201 ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg tatgtgtgtc
     7261 aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc tcgttctttc
     7321 actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa tggattatta
     7381 gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt tgttacgcag
     7441 ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc attgtggatc
     7501 gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt attagctaat
     7561 atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa cagttcttat
     7621 gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc attcctcgcg
     7681 aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt attcaatatc
     7741 aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc ggttccgttt
     7801 ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct ggaaatcgtc
     7861 aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc atttgttggt
     7921 agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat cgcattcatt
     7981 atctggttcc catttatcaa attctatgat aataaattat taaatgaaga aagagaaaag
     8041 gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga actgtgacaa
     8101 aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa ataccattat
     8161 tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc tacaccattg
     8221 attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg cggagcgctt
     8281 tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac gatcaaatct
     8341 ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc aaatgctggg
     8401 tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc aaaatcatgt
     8461 cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg gccatctgga
     8521 tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg tgcatagtcc
     8581 acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg tacacgagct
     8641 tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc ggcaatgatc
     8701 gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata agcagtatcg
     8761 cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc tttatctgaa
     8821 cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg aatctggaat
     8881 agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa aacaactgtt
     8941 tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt cggatgttcg
     9001 atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa agtcaagccg
     9061 tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg agtcatacca
     9121 ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt tgtcaaatgg
     9181 gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa tgctgttttt
     9241 ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt tttacctttt
     9301 gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa agccatagca
     9361 agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga attttcgtat
     9421 ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa atattcttct
     9481 ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct tgaatcataa
     9541 cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct tgttgaaaca
     9601 aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa agagaagctc
     9661 attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact ctttttaata
     9721 tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg acacagggga
     9781 caagcggatg agtattttca taaacaatga actgactgta atagatagga gaacgaatag
     9841 tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat tggtaagtaa
     9901 aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg gtatcgttat
     9961 accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga acaaaagact
    10021 tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa caatctctaa
    10081 aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt ttcctaatag
    10141 gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg agcgttggat
    10201 agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag ctatatcgtg
    10261 agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa atataacggg
    10321 tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat aaaaggctgt
    10381 atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt gttcttataa
    10441 aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata gtaatgtttt
    10501 gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag atcaattaac
    10561 accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata tcatttcaag
    10621 attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa atgctaaggc
    10681 tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac tgatctctac
    10741 agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat tgaatgaata
    10801 tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt taggcctgtt
    10861 gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg gtggcggaga
    10921 agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc cttgtatgag
    10981 tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat
    11041 tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca tcatgaaagt
    11101 catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat ttatcggtat
    11161 gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt atacatcggc
    11221 gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt tgttagatac
    11281 cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct tcaaattagt
    11341 cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca atgcacagtt
    11401 actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg tagttattgc
    11461 attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg ttacgttgaa
    11521 agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt cattttttat
    11581 gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat tattttatac
    11641 accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac tcttcctagg
    11701 actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg cggcaattaa
    11761 ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca ttcgtttatt
    11821 tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt gtaccttgat
    11881 cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc gcaggacatt
    11941 gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa agatgatttt
    12001 tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc ttgtagtagc
    12061 aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg cagaaagact
    12121 actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag tgaatcagaa
    12181 gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata agtttttagc
    12241 agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag gaacgatcga
    12301 agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg aagatgcggt
    12361 tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt tgcataaacc
    12421 tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg atttattgga
    12481 agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag acacagaggg
    12541 cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac ctaaaaaaca
    12601 tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag atatccgttt
    12661 atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa aactccaaat
    12721 tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc atgaaggaaa
    12781 gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag
    12841 aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat atcgacccat
    12901 gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc taaaagaata
    12961 agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt tcggatggct
    13021 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tttgttcgga
    13081 ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga ctcagagtta
    13141 gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca caacctctga
    13201 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
    13261 actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca caacctctcc
    13321 tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg aaagcgctat
    13381 aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc
    13441 atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta ccgggtatgc
    13501 tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt
    13561 ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg gtgggttggg
    13621 aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa aatacaattt
    13681 tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt gcaatcatta
    13741 tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc ttactacctt
    13801 attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt cctgacaaag
    13861 gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg tataatgaac
    13921 cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt ggttataaca
    13981 gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa gctgctatgg
    14041 ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa cttattccac
    14101 taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat tttggcctgt
    14161 tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt ttggatacgt
    14221 atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct gcaggacttt
    14281 accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg
    14341 atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt
    14401 ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga ttgcactctt
    14461 tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta ccgatgagtc
    14521 catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat ttggttatac
    14581 gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca ctgtacttgt
    14641 tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt atgcgatttc
    14701 acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta cgatgttgtt
    14761 ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt taaaagatac
    14821 gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg tgatgcgcac
    14881 attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg atggagctag
    14941 cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta ttgcgacaat
    15001 cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac tatacatcca
    15061 agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta atattcaata
    15121 tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct cagttccagg
    15181 agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga tcagctatcc
    15241 atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa
    15301 caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag aaactagcct
    15361 ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt tctggttcta
    15421 aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc ggagataaac
    15481 cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa gagatcggtg
    15541 taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg aacgtcatca
    15601 tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa
    15661 aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa gcttataatg
    15721 atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat gctttcccag
    15781 tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta ctagataaat
    15841 acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc ttgaaggaat
    15901 tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc aaagtccaag
    15961 ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg gatggagatg
    16021 attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta cgtacaatgc
    16081 atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat aaggattatc
    16141 cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat gattatggcg
    16201 atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt actgttccac
    16261 taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact tcaaaaaata
    16321 aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta ttgaatgggc
    16381 ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat gataaaatca
    16441 aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca ggaaacaaca
    16501 agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt gatcaatcga
    16561 ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac agtgtcaaaa
    16621 cagaattaag taatatttct aatgtcatga atcaatattt agatggattg aatacaggga
    16681 ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag gctggctatg
    16741 ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa taataatgaa
    16801 taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga ataaacggtg
    16861 ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga aagccatatt
    16921 cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct tggtaaacgg
    16981 tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga ggaactattt
    17041 ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt tttttgtata
    17101 atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg ttttggaaga
    17161 gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga tatatacatt
    17221 tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg aactatttta
    17281 tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata tatataaaag
    17341 aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg ctttattgag
    17401 ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta ttgattttat
    17461 tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga ttttagatag
    17521 tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg ttttttccag
    17581 cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg tgacttggca
    17641 attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga atggtacgat
    17701 gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat aagtttcgga
    17761 ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg gttatcatga
    17821 tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa caaaatcaaa
    17881 aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg ctgaatgata
    17941 aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa
    18001 atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta tacgaacagc
    18061 aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc tacgcaaatt
    18121 atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat tatgaatctt
    18181 ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta gacgcctttt
    18241 atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt attggttttc
    18301 aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga ctctctttat
    18361 acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa atagctaaag
    18421 aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat tcacaattgc
    18481 cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct gatgattatc
    18541 gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc
    18601 cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt tataaaacat
    18661 tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa acagcagcag
    18721 ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag gaactttcaa
    18781 tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc tacaaattag
    18841 aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat ccacactttt
    18901 tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt gaagaattag
    18961 cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga
    19021 tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt
    19081 atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag atcgaagtac
    19141 ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt gattttactc
    19201 gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta aggatcatca
    19261 tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa gaaaaactgt
    19321 ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat gaacgtttac
    19381 gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca ggtggactga
    19441 ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg tagatgatga
    19501 atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag gctttcaaat
    19561 agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc gacagattga
    19621 tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta ttgaagcggc
    19681 tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag aattcgatta
    19741 tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg tgaataagtt
    19801 cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa atgaacaaaa
    19861 aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg aagaattgga
    19921 tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata ggatagtatt
    19981 gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag aacggcaaca
    20041 aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt tgttggaggc
    20101 agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc aggaatggct
    20161 gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat tccaacaagc
    20221 gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg ttttttacgc
    20281 agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac gcgtgctgca
    20341 aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac agtttcagtt
    20401 agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta tggagattca
    20461 acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa aaatccatca
    20521 ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa agcatcagag
    20581 acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc agcattatca
    20641 agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca tgtatctagg
    20701 acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc atttacgtat
    20761 ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga tagcaagtga
    20821 gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg tcggcagatc
    20881 ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa tgtaatgaaa
    20941 taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac ttgaggcaag
    21001 gcagcttttt catgacctct cgataaacgg
//
LOCUS       DACEJZ010000051        20772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEJZ010000051 DACEJZ010000000
VERSION     DACEJZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.052"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(436..1749)
                     /locus_tag="IW656_001701"
     CDS             complement(436..1749)
                     /locus_tag="IW656_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0837412.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1994..2218)
                     /locus_tag="IW656_001702"
     CDS             complement(1994..2218)
                     /locus_tag="IW656_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837413.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2238..2606)
                     /locus_tag="IW656_001703"
     CDS             complement(2238..2606)
                     /locus_tag="IW656_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0837414.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(2802..3512)
                     /locus_tag="IW656_001704"
     CDS             complement(2802..3512)
                     /locus_tag="IW656_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837415.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3530..4026)
                     /locus_tag="IW656_001705"
                     /pseudo
     CDS             complement(3530..4026)
                     /locus_tag="IW656_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4063..4515)
                     /locus_tag="IW656_001706"
     CDS             complement(4063..4515)
                     /locus_tag="IW656_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0837416.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4650..5255)
                     /locus_tag="IW656_001707"
     CDS             complement(4650..5255)
                     /locus_tag="IW656_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0837417.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5705..6325)
                     /locus_tag="IW656_001708"
     CDS             complement(5705..6325)
                     /locus_tag="IW656_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0837418.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6327..7196)
                     /locus_tag="IW656_001709"
     CDS             complement(6327..7196)
                     /locus_tag="IW656_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0837419.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(7219..7626)
                     /locus_tag="IW656_001710"
     CDS             complement(7219..7626)
                     /locus_tag="IW656_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0837420.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7645..8934)
                     /locus_tag="IW656_001711"
     CDS             complement(7645..8934)
                     /locus_tag="IW656_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0837421.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8961..9263)
                     /locus_tag="IW656_001712"
     CDS             complement(8961..9263)
                     /locus_tag="IW656_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0837422.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(9288..11381)
                     /locus_tag="IW656_001713"
     CDS             complement(9288..11381)
                     /locus_tag="IW656_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0837423.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11870..12598)
                     /locus_tag="IW656_001714"
     CDS             complement(11870..12598)
                     /locus_tag="IW656_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0837424.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12744..15365)
                     /locus_tag="IW656_001715"
                     /pseudo
     CDS             complement(12744..15365)
                     /locus_tag="IW656_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(15479..16810)
                     /locus_tag="IW656_001716"
     CDS             complement(15479..16810)
                     /locus_tag="IW656_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0837425.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16776..18254)
                     /locus_tag="IW656_001717"
     CDS             complement(16776..18254)
                     /locus_tag="IW656_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0837426.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18559..20394)
                     /gene="tdc"
                     /locus_tag="IW656_001718"
     CDS             complement(18559..20394)
                     /gene="tdc"
                     /locus_tag="IW656_001718"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0837427.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            20621..>20772
                     /locus_tag="IW656_001719"
     CDS             20621..>20772
                     /locus_tag="IW656_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0837428.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTA"
BASE COUNT         6035 a         4449 c         3484 g         6804 t
ORIGIN      
        1 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
       61 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
      121 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt attcagggaa gaatgctgat
      181 ttcacaactt ctttataaac ggtgttcact cagctgtccc tcggtattag ctcaaaaaac
      241 ggcaaaatat aagaaactat ttttttcgtt tttttctcta atactcaggc cagaacactg
      301 atttcacaac ctctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa
      361 aaggcaaaat atgaggaact attttttcgt ttttttctct aatactcagg ccagaacact
      421 gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccatga atacaaagaa
      481 cagaccatac atgatgggag atacttcttt tcctctttta gctgctaaca tggtaattgg
      541 atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat
      601 caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga
      661 ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac
      721 tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt
      781 gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg
      841 agaagtacct aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc
      901 tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt
      961 tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag
     1021 agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc
     1081 tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt
     1141 agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac
     1201 gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa
     1261 tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag caataaatag
     1321 accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc
     1381 ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa
     1441 tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat
     1501 cccaagagca ggtgccgttc caattggata acgggctaat acccccatta aaatacagcc
     1561 gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc
     1621 taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc
     1681 cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt
     1741 ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg
     1801 atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa
     1861 attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt
     1921 catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt
     1981 ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga
     2041 tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca
     2101 tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct
     2161 cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg
     2221 tttctcctcc tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct
     2281 tcagggtccg aaaaattgaa agtcaagacg ccattgttgt ctgtgatttc tcctggttct
     2341 tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt
     2401 cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat
     2461 tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa
     2521 atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa
     2581 agcatcacac gtgttttttg tatcatcgtt tgacccttct ttctatttga ttttctttcc
     2641 tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt
     2701 caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa
     2761 aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat
     2821 tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc
     2881 ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg
     2941 accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg
     3001 agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga
     3061 gcatcggaat ctgcacctaa aggcaggaat gattcgtcac cttgggcttt tcctaaatgc
     3121 agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa
     3181 atactattat agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca
     3241 cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat
     3301 gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt
     3361 cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt
     3421 acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc
     3481 cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat tagaataatt
     3541 gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt
     3601 atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac
     3661 cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa
     3721 atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa
     3781 aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag
     3841 gctcttgatc gatactcact aggaaaaaat ctgtttcttt atcttttagc tcttctgtaa
     3901 gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt aattatcttt
     3961 tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta catctgaagg
     4021 cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga tccttttatt
     4081 gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat ttaaaagata
     4141 ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat tggagtagtt
     4201 gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat agagtttctt
     4261 gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt ctaatttctt
     4321 gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt tttcttggat
     4381 gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg ttaaatcgac
     4441 ttctttgaat tcgatattgc taatagagtc tagcgctcgg gcaatcattc cgatgtctcg
     4501 aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt tacctctttt
     4561 tgttgcaaat gcaactattt ttaataaaaa aatcaagttc ttttttaata actagtaaag
     4621 tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt tttgttttct
     4681 aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa ctcttctata
     4741 ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc ttcaccgtgg
     4801 atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc tttttgttta
     4861 ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt ttcttcatag
     4921 acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc accaggaagt
     4981 gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt tttgatatag
     5041 gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag cgcttttatt
     5101 tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt ccggatctcc
     5161 tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg agaaagtgcg
     5221 agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt tttagcaaat
     5281 gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa atataatgaa
     5341 atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc gacttctctg
     5401 gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc catcaaacga
     5461 aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg acagcttttt
     5521 cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag tcacaatatt
     5581 catcaaacat gagaagctgt ttctttgaaa ttgctccttg tgactcggag ctgaacgaca
     5641 ggctcacgac ctctcggtaa acggtgttca aaagccaatt tttcgatatt aagccatttc
     5701 tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga gtgtaatcca
     5761 cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat ttgtcatatt
     5821 ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc cctttcgttc
     5881 gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa tgacgatctg
     5941 attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct gttaggcgaa
     6001 tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct ttttgcttat
     6061 cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac aaatcaagtg
     6121 ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct tccaaattgt
     6181 taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg gcatacatat
     6241 ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca ggactaccag
     6301 ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg actttccaag
     6361 ttcataaaca gtccaatctt atgcttgaca atttctttta ctgctttatt tgctggaatc
     6421 acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt agctactttc
     6481 gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt attgatcaat
     6541 tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg gacatcaact
     6601 actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt gaattgtcca
     6661 tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac aaatttcact
     6721 gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc ttcttctgta
     6781 ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc atattctact
     6841 actgcttttg tcttcaagat attttctttg aaaggtagat gggacccgtc atacatgaca
     6901 gaagtatatc cgctatcgat tgcttctttg atatcatcaa agtctcgggc gtggtctaag
     6961 tgtaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt gactaacaca
     7021 tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgattgg cgaacccatt
     7081 tcttttgctg cgcctagcat ggcaggaagc atttctaggt tatgcgtatt aaaagctcca
     7141 actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt atacatcttt
     7201 tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg caatttgtcg
     7261 catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag cacgagcagc
     7321 atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc cgatcgtctg
     7381 tttattttcc aagctctttt ctaaatattg gatggcttga tcgttctcat gcaattcaga
     7441 aaatttaagt ccaacttgtt cataaagttc cgctaattta tgcctatctt gggtagtgga
     7501 aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt ctggcgatag
     7561 ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct taaaaaatcc
     7621 aaacatcatt ttccctactt tcaattaaaa cgctttcaaa actgggctta agatccaggc
     7681 atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattga tgaatcccat
     7741 cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac catgagcaat
     7801 accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc
     7861 tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga acatgggcaa
     7921 agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa tgacggcatt
     7981 tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag gaactaattt
     8041 catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta caccagctag
     8101 caatatatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt
     8161 ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag tgatgatata
     8221 tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt
     8281 cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa aaagcttagc
     8341 aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa tatcatttga
     8401 tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg cgaaacaagc
     8461 tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa aatagccaaa
     8521 gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa tgtacttcca
     8581 ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg tcagtgcaat
     8641 atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa tagaagggat
     8701 gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta aacttccaac
     8761 gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta acgtgccaga
     8821 gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa tcaatgcaat
     8881 ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc tcatgttttt
     8941 accctctttt ctttttgtat ttagataact cccttatctt gcagaactgg ggtgattttt
     9001 tcttcaattt cattttttga aacaatattt ttcaaataga tcatcgccgt tttaggatca
     9061 actgcgaact tttcgaactg cgaacgaaaa ttttctgctg tgatgatcac atctggtttc
     9121 atggaaactg cggaagaaat atcacaatgg tccagtttgg cttctactcc taatttgttc
     9181 aaaacatcct cagcagacat ctgtgctgca aaactacttc ctaaacctgc gccgcatacg
     9241 aataaaatgt tcaatttgtt catctttgtt ctcccccgta tcatttatta tttctcaact
     9301 actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc attcaatcaa tcgatccaac
     9361 ttctcttcgt tgttgatcaa ttcaattagt tctttcatga tgttcagatg ggagtaggag
     9421 tctactgctg ccaaacaaaa aataatcttt actggatcca tttcaggatt accgaaatcg
     9481 attggttgct caatcgtagc tacgctgact cctaatttat tgaccccatc ttctggtcgg
     9541 gcatgggcta gtgccatgtg tttgccaatc acgatatacg gtccgtaatc attcactgca
     9601 cgaatcatcg cttcgacata gcgctcttcg acaatgtttt ctttaaccaa tggtctagca
     9661 gctagttcaa tcgattcctc ccaagtagtg gctttttctt tgatcatgat attgctgtcc
     9721 gtcaaaatat cttttaacat aggctggatc tcccttttgt tgatttctaa attgtttgtt
     9781 tcgaataatt tttctaactt gccatagatg attcgatcta ctcggccacc actttctttc
     9841 atgacatcta ttatttgagt gaataactcc gtcgattcat gactgttagg ctctaaccgc
     9901 tgtagttctt catttctttt caaaaagtct tctataacca agatattcgt ctcagttaaa
     9961 atcggatcga caactaaaag cggcttattt cgataattca attgaaaggt gctgaaaacc
    10021 agattgacat ccaatttatc tactaattcg atttcttttg aacttaaaac tgcagtaatc
    10081 tcgatctgag gaaacttttc tttcagattc tctgctaaca gatttccagt agccatccca
    10141 tggttacaga caacgactga tttataaaca taattcaagt ctcgcttgat tacactggcg
    10201 atcgtcgaaa agtggatgac aagaaaagct atctcgtcct ccagtatctt gccaccaatt
    10261 acttcttcga tcgtaggaac aaaatgagtg actgcgcggt agattgggaa ataatttgtt
    10321 cggatgtttt ctttcaatgg attcatgact tggatatgat tcttgattct tacaatcagt
    10381 gcagccatgt gcttatagag attttcgaat aaggcttctt cctttagatg gaaaggtacg
    10441 tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta gtaattgagc ttgtacccac
    10501 tcgattgaat tggagatatc tctagcattg aacgtctcaa tagtgaaacg aagataattt
    10561 ctttctactt gcgggacatt gtccaattga aattcctcga tcatgtggtc aataaatgca
    10621 gaaaagctac tgtcttttat ttctgtattt ttccaattaa tggcagagat aaattcatgt
    10681 ttcctgattc gctggatcca gatcaatgta aaaagcagga tttgtttttt ataaatatca
    10741 tcttcttgtt tcaacaactt attggaatag atctcctgta tttttttgat ttcctccaaa
    10801 ggaaaataat ggagaaagat tttttgagcc ggtgttaatt gtgcatcttg attcataaag
    10861 aagtcggtca ttcccagatt ctgattgata aaatcataga tcatcgtacg gatagatcgt
    10921 tctgaacctt tataaaccag tccttgcttt ccataactga cgatctctat cccataactg
    10981 accagtgttg ctcgtaaccg tctcatatct tcatctaatg tacttttaga gatttgaaat
    11041 tcttcttctt ttttgtttag atggatcggg attcggctgt aagcaacggc taaaactaga
    11101 tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca catcttttat ttcagtttct
    11161 aatcgagctt gcttttcctt ttccagcttc aacaaaaaac ctttgttgcg aattgtacgg
    11221 atctccggaa gctttttact gctcaaccag atattcgctt caagaacttc ctgacgaatc
    11281 gtccgctgac tgacattgaa ctcacttgct agatgttttg cttttactgg atattgggct
    11341 gtcatgattc tgtgaagtat ttcaattgtt cttgttttca tagaaatgat ctccttctga
    11401 tttcaagtat atggttccgc ttacataata aaaagtagat atttctgcca cttgaagtgg
    11461 aaagaaagta agcaaagcaa acaaaaaata agccatatgc tttttcatct cctcaacgaa
    11521 aaagacatac gcctattatt acaaaaattt tgcccatcta tacaaaaaag aggttgtgaa
    11581 aaagctgtcc tgcatcaagt aataagaaca actaaactaa aatagtgctt cctatttttt
    11641 gtttggttgt tcttattatc gcagatgcaa gcttttaaac accatttagt ggagaagctg
    11701 tgacagagat gttcagcctt gagcgataag aagcgatttt gaaaaatcgc ttcccatatt
    11761 ttacaaaatc gtgccttatt gccgataggc tatctctgga acaccgttta ttcagaaaaa
    11821 ggagctgcga caaggctttt gtcacaactc cgttcataaa tatttcctgt caatccagca
    11881 taatcggttg aataaaatcg gcttgccatc ttttttcttt ctcagaaaaa tcaaaatacc
    11941 atatctgaga aaaatactgt cgttgtgtct tacttcctga gatcaatcgc ccatcttttc
    12001 gatcgactga ataatcacag catctgaaat caattcggat ggcaaaacta ttttcgtgga
    12061 tctgtcgata atttcctact tcctgaatag ttattttttt agtatgattt cttacaccgt
    12121 ctgctttgtt tttttgcaga actttctgat gttcggcaaa cagcttgtct gtataacagc
    12181 ttctggcttt ttcaggagtt tcttcttccc aagcggattg gatcttcatg aatatctttt
    12241 ggatttccaa aaactgtgct ttcttttctt tagccgttcc tgacatgcgg gacaatatct
    12301 ccgatttcag ccttttcttt tgtcttttat tcttgacgat agaaaaagca gaaacccctc
    12361 ctactgacaa aacagctaat gcaagaaacc ccgtttgctc ataactagaa ggacctgtac
    12421 ttggaccata atacccataa gaatctctgc cgtaagaacg actgccaaaa taactgcccc
    12481 catatgaacg accgccaaat gaacctctgc ccgaacttcg accgctagat gtacctccac
    12541 catgaccacc cgcgacagcg aatccttggg tcggcagagc aaacaaacat aggcccacta
    12601 acaaaatgat tgctaaataa ttgatttttt cattgtcttc ctcgttatct tttattacaa
    12661 aaaactattt ttatcatagc cttgctaaaa ggaagagtca ataaccagat gcataaactg
    12721 tttttttagc aattttgtct tatttagtca ttgagtaaca tcggacagat gtatgtgtcg
    12781 gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag atctgcgctt
    12841 cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa tggacgattt
    12901 ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa cgttttctga
    12961 tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa caacatatgc
    13021 atttgtttct gtacatatga atcattatct agctccttag ctgcttggat tgcaagcctg
    13081 atatgcggat accctaatat cgcttcttca tatgcaccat ttacgccata tttttgcttc
    13141 accctatgac catgagtggt ttttttatga cgatattttg tatcaggaaa agcagcaagc
    13201 ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact gattttttgt
    13261 gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt attgatgcca
    13321 tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat tctttctctt
    13381 aactcctgat caatagcata atgccatgct atttgagcca tttcttcaaa aaaaggagtt
    13441 agcgtcttag cagacttatc caaaatgcgc cgaccttctt ttacggcttt tgcgatcgta
    13501 tggaaattat catctcctaa aaccatatca gctgcctgtt tgcttacttc actacctttg
    13561 attcccatag cgattcctat atcggctctt ttcaaagcag gtgcatcatt tacgccgtct
    13621 ccggtcattc cgacgatttc tccatttttc tgcaaagctt tcacgattcg cagtttgtgt
    13681 tctggtgtcg ttcgagcaaa gacgtcgact tttggtactt gtcgaatcaa ctcttcttca
    13741 gacataagat ccaaatcgat cccttctaaa actttagcag tatgttttaa gccaatttgt
    13801 tccgcgatcg cttttgctgt atctttgtga tcccccgtaa tcattttcac tgagattcct
    13861 gcttgttggc attcctttac agctttgact gcactttctt ttggcggatc aatcaaccca
    13921 gcaatccctg caaaagttaa ttgggttaaa gactcatgcg tgagttcctg tttcgaatct
    13981 acttttttgt aagcaaatcc taaaacacgc tgaccttttt tcgcaaacgc tgtggctgtt
    14041 tgaccccatg cttcttattc tgaagaagaa agagttgatt tagccaataa tacttctggc
    14101 gcacctttga cgtaaatcac ccctcctttt tcctccgcat gcatcgttgc catatatttg
    14161 tattcagagc tgaacggaat ttttgcttct atttttttca aaggcagatc ggctgtttcg
    14221 gtgtggcgaa tcagtgcaag ttctgtcgga ttgccactta aattttctgt tttctgttct
    14281 tctttcgtct ttaattcctg acaatttttc ataataaaca gtacgcgagt ttcttccttt
    14341 gctgctacat ccacaactgt catttcattt tttgtcaatg tccctgtctt atctgaacag
    14401 atcactgtca tagaacctaa tgtttctaca gaaggcagtc ctttgatgat cgccttttct
    14461 tttgccattt catgtactcc gacagatagg atcatggtca acacagctgg caatccttcg
    14521 ggtaccattg acaccaccaa agcgatcata gctgaaagaa ggatatgcca atccatgcca
    14581 tagcggaatg ttgtaaaaac aactaagaac aagatcaaac aaagaatgcc ttgaaaaatt
    14641 tgtttgttca atcggttgat cttcttgatc agaggcgtcg tttgttgttc gaccgattgc
    14701 agtgcttgat tgattttacc gatttctgtc gtgtttcctg tttctacaac gacaccgact
    14761 gcagaaccag attgtacaag cgttcctgaa aaagccatat ttttttggtc accgattgat
    14821 tcttctgctg ccaaaacgga atgatttttt tcagatgctt ctgattctcc cgtcaaaatc
    14881 gattcttcta ctagtagatc atgtacttcg atcaaccgaa catctgctgg aacgacgtct
    14941 ccggcacgta agacaatgac atctcctttt actagttctt tcgcagggat tttttcttgc
    15001 atcccgttcg atagtactat cgcttcttgt cccatcagct gcttcaaacc attcaacgat
    15061 tcttctgctt ttctttcttg ccaataacta acgagtccgt tgacgatcac tacgaataaa
    15121 ataatacttc cttcgatgta ttcgctcgaa gcaaatttca aaaaagaggc aatgatcaac
    15181 acgatcatca gcagatcagt aaagtgtttt gctatttttt tccatcgttt tgtctgctgt
    15241 cttgcttcta tctcattata tccttgatca gtcaggcgtt tctgccgttc ttcttgcgac
    15301 aagccttctg ttgatgtttg atagtttttc aatacatctt ctaccgtcaa tctgtaccag
    15361 ttcatcctat cccctcctgt ttaattgata ctgttattat acgcagggaa gttttttcag
    15421 gcgtatcgat tatttcaggg gggtctgaaa taattcgttt tgcagaatgt atctgggcct
    15481 aactgatatt tctattgctt attcttttat atacaaaatt taagaataca aaccagataa
    15541 ccaataaaaa aggaataaag cgtgtcgtgc tttcgaataa caagctgaaa gcaattgaag
    15601 taaaaaacac caacaaagca agatctttat atctttgtgt agtgttccgt ttcttttctt
    15661 taataaattg aacatgtgca cacaagatcg aagaccacac aaatagaaaa caactcgttg
    15721 tcatgcctgc caaaagctga aaaaacaatc gactatcagc tacaaaaaaa tgtagacaaa
    15781 gaccgacaaa aaataacatc cctgtaatta aaataccgtg taaaggtacg cctttcttag
    15841 aaagcgcaca aaaccaatgg ggcaaacttt tctcttttgc tgtttgatat aaaatcctgc
    15901 tagtactgta gactgcacta tttgctgagg acaaagaagc aaccaaaatc attagattga
    15961 caattgctgc tccgctttgg caatggatca cttgcagcat agtcactaaa ggactttctt
    16021 ttaaatctaa acaatgccat ggaacaacaa ataaaatcga caaaatcgtt cctatataga
    16081 aaaagaggat acgccaaggc agacagttga ttgcattttt caaattcttt tgtgggtcac
    16141 tcgtctcacc aactgttaat ccgatcattt caattccagc aaaactgaat aaaaccatag
    16201 gaaatgaagc aatcattcca gcaaagccaa acgggactaa ttcattgagc tgtccaaaat
    16261 ttgctttgat tccaatagac tgggtatgaa aatctgagac actcaatcct atacccaata
    16321 agataaaaag gaaaatcccc aagatcttga tgattgagaa aaaagattcc aattcaccaa
    16381 accacttgac tgcaacaagg ttgactgcca ttaagatcat tagtgcgcta cctccgctta
    16441 tcaaagctgg tacatgtaca aaccagaaat ggatatacaa tcctattgct gttaattccg
    16501 caagtccagc tccaagccag caaagccaat aagtccaatt gacaaaaaat gcccactttt
    16561 tgcctaaata atagtcaacg aaatcactga tagagttaaa tgtactattt gctaaaaata
    16621 aatcacccat gacccgcatc aaaccataaa tagccaaacc gcatatcaaa taaacgagaa
    16681 taacagctgg ccctgctgat gcaattgcct ttgctgaacc taaaaataat cctgttccaa
    16741 ttgcgccacc taatgcgatt aactggatat ttcgattagt tagagtccgt ttcattgccg
    16801 gtttttccat caagttcacc tttttctttt tcaagcagca tggcgacttc ctgttcccaa
    16861 gaaggtttct ttattttttc aatacagagt ggtactacaa acaaaatgac actgatggct
    16921 gtgactgcaa ttagattgat ccatgtccca gctttcatcg tcagtccgac agaaaatccg
    16981 attgccgcaa ataaaacaat aacgacagtc caaactccaa gattgctctt tttctctttt
    17041 tttccaattc taaatggtcg attcaacttt gcatgtgtat aacgcaatct caaaactgcg
    17101 atccccatga tgacatagac gatacagtag ataacagtag ttgaatttac gagcatcaaa
    17161 aatgcttgat tgacgccagg aatcagcaaa taaaaaagtg caaagattga gataatgaca
    17221 gactgagtca aaataacgga tttggatacg tcaaattgat tcgtcttcca aaaattgaat
    17281 tttggtggat acgcaccttt tctagcactt tctgtcactg tctttgctgg tcctgaggcc
    17341 caagcagata attggactgc tacaccaatg aagacaagta aactaaacag attcacaata
    17401 agatgtggca aaccaagaat ttggcaataa atcgaaatag attgcgcgat attattcaat
    17461 tccatttgtc cttttggtac gacatttgct acaactaaag cattcaaaat attgacgaaa
    17521 aatataaaaa ttaacgcggc aaatactccg cgaatatacg ttttaactgg atttttcaac
    17581 cgagtgatat acacagagga catttcgata ccagtaaaaa taaacatgat cggcgcaaaa
    17641 taaacaaatg aggatgcagt cgtagtatct ggtattaatt tgtcccatga aaaactgcct
    17701 aatgttccgg ttggtacgat cccaactttg atccacgcag caaaccctaa taagagcatc
    17761 atcgtcaaag gcacgtacaa tcctaaccaa acaccgactt ttccgccgat ttttgccata
    17821 tcaaatttca aattcaaaaa agtgatgatc caataaacga ctaaaatgac aactaatgta
    17881 aatttgctgt tcaaaccaag cggcacatta tcgataatat taccaaacaa cggagcaagt
    17941 gctgaagcga ccatcaccat cccaggaaac atttggaccc ataacagcca tgagacaacg
    18001 aacccccatt tttttcctag agcattcgat acccaaagtt ctggaccacc tttttgggga
    18061 aacattccgg caaattcacc agagatcaat gagattggaa aagcgtacaa taatactgca
    18121 accagcatgt aaaaaatcat cgtccagctc gttgctgcta catctggaat atttcgtaca
    18181 cttgctacaa gggcgcaggt cattccgata aaggtcatgg tcgtgatttg ctttgatgaa
    18241 ccagtatctt tcatctttat tccttctttc aatagcgaca tatgtttttg ttttctttta
    18301 tttttgttca agaacagaaa tgacaaggtt tcatcgattc agtacaaaac ccagtatcac
    18361 cgaaacatcc attgaacgat cttagtgaac tgggtttgat acatgaaacg aacgatccaa
    18421 cttatagaac gttttataaa aaagaatatc ttggcgagga atatatgaaa aacaggggga
    18481 aatacaattg ttttgatgaa gcaaataatt gtctgttctc catatgtctc ttgttattct
    18541 ttcacacaaa aatcagttct attggatgat ttcgtttagt ttcttttcca ttgctttggc
    18601 gatttctttt gtataaaaat tgaaaattcg atcatcattc aaataaggag tcatgatagc
    18661 cgcacgtagc agagtgattt tctgttcttt tttccattct tctttcgtta gacccattct
    18721 ttcaacaaaa cgaatcggag aatcaccgta atcttcttgt gtaaatgtag tatgagaagt
    18781 aataaaccgt tcaccataaa catcgccatc catataagaa gaccgatcga acattttttc
    18841 atttaattca ttgatagcgt tcaaatctgt acagccttgt tctttgaata cccaattgac
    18901 catattaaaa tcaggatgat tcaatggata gacttcgatt gtttttcctt taacagtaaa
    18961 agtcagatga tcgagaaatt ctctgaatct ttgagctgcc tcgattgaag ccccaatcaa
    19021 ttgaccatat ccggtcacat tcaatggcaa tacacgatgt gctgtccaga ctgcggcggc
    19081 ggttgctcca gcttttgatc cttccaaaat ataagctcct aacatatctg gagcctttac
    19141 tgatttttca aacacatatg gcgcgaaata agaaatgata tttctcatat ttttatgttt
    19201 gattacgatt cctccagcag cataagggac atagcccatc ttatgaggat ctatcgtgac
    19261 agaatccgct tctgaaatag ctctgaaccc ttcatatacc tctttgtcaa tcgttacgca
    19321 gtggtggaaa acatgatgtt cttcaaagaa ttcagctaag gaagcatacg gcacaaattc
    19381 tcctgcttcg tttagaaaca gtgaacgcgc atagccgcca tatgcggcat caacatgtaa
    19441 atagaaatag atcccttcat cttttaacct ttctcttaat tggacgattt tatctacact
    19501 gtctacttga ccttcttctg tcgttcctac gacagcaacc acacctagaa ttgggatttt
    19561 ttgttcagct aattcacgaa ttgttttttc caaagcatta atatccattc gataatcctc
    19621 ttgaactggg atagcaacca tctgatctaa gcctactccg caaatatcta acgccttcat
    19681 ccaagaatag tgttttgttt gaggcaccag ccatttccct aatctttgga tattttttcc
    19741 gctacgggaa gaagctgctt tgacctcatc catttcttcg tccgtaaagc tttcggtcat
    19801 ttccaaaatt tcttcaacag ataaattcaa aagttcccac tcagacattt ttttcacttg
    19861 ttcaggcagc acttccttga cagctaaagg aatcgatttg atgcagcggg cgtaccataa
    19921 tccttcaaga ttagcaatag aaccatctgc tgcaatgtgt ccccatccgt ctgtcatatt
    19981 gaataatgaa gcaaaatcct gtcctacttc tgcttccatt tgcgaagttg ccatagaaga
    20041 ttccaatgca acattatttg ggttccacag catcgcatag ttgtaagcta ataaagcagg
    20101 catcaatgtt tcagcattca tttgtcccca gtaacggcct gctgaatgcc atgggatcga
    20161 tcctgcgcgt aatcgctgac tgacttcatc caagacctct atcatatggt cgcgggtggc
    20221 tagataatct ggagtgagct tatctccttc gctaattgca ggcatatctg aaggaatata
    20281 attttctctc catccaagat gttcatctac catcttgttc aacaacattt tataaactgg
    20341 tccattttca gctttgtcgc ctataaagac ggccttgata tccatatctt tcatttttct
    20401 tccctccata ttttgttggt caatgtctcg cagatacatg tgtttatctg taacacctct
    20461 tcactatagc gccaaaaatg agaaattttt ttttataaca tcaaatccac gtagattttt
    20521 acaacgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    20581 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    20641 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    20701 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    20761 aggcaagact gc
//
LOCUS       DACEJZ010000052        20261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEJZ010000052 DACEJZ010000000
VERSION     DACEJZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.053"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            293..1432
                     /locus_tag="IW656_001720"
     CDS             293..1432
                     /locus_tag="IW656_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0837429.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1499..2074)
                     /locus_tag="IW656_001721"
     CDS             complement(1499..2074)
                     /locus_tag="IW656_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0837430.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2079..3053)
                     /locus_tag="IW656_001722"
     CDS             complement(2079..3053)
                     /locus_tag="IW656_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0837431.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3077..3121)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3336..4304
                     /locus_tag="IW656_001723"
     CDS             3336..4304
                     /locus_tag="IW656_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0837432.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4417..4695
                     /locus_tag="IW656_001724"
     CDS             4417..4695
                     /locus_tag="IW656_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0837433.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4813..5049
                     /gene="secG"
                     /locus_tag="IW656_001725"
     CDS             4813..5049
                     /gene="secG"
                     /locus_tag="IW656_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0837434.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5161..5907
                     /locus_tag="IW656_001726"
     CDS             5161..5907
                     /locus_tag="IW656_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0837435.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5910..8267
                     /gene="rnr"
                     /locus_tag="IW656_001727"
     CDS             5910..8267
                     /gene="rnr"
                     /locus_tag="IW656_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0837436.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8289..8753
                     /gene="smpB"
                     /locus_tag="IW656_001728"
     CDS             8289..8753
                     /gene="smpB"
                     /locus_tag="IW656_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0837437.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9055..9774
                     /gene="atpB"
                     /locus_tag="IW656_001729"
     CDS             9055..9774
                     /gene="atpB"
                     /locus_tag="IW656_001729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0837438.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9827..10042
                     /gene="atpE"
                     /locus_tag="IW656_001730"
     CDS             9827..10042
                     /gene="atpE"
                     /locus_tag="IW656_001730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0837439.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10133..10657
                     /gene="atpF"
                     /locus_tag="IW656_001731"
     CDS             10133..10657
                     /gene="atpF"
                     /locus_tag="IW656_001731"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0837440.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10644..11186
                     /locus_tag="IW656_001732"
     CDS             10644..11186
                     /locus_tag="IW656_001732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0837441.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11209..12765
                     /locus_tag="IW656_001733"
     CDS             11209..12765
                     /locus_tag="IW656_001733"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0837442.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12779..13681
                     /locus_tag="IW656_001734"
     CDS             12779..13681
                     /locus_tag="IW656_001734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0837443.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13704..15110
                     /gene="atpD"
                     /locus_tag="IW656_001735"
     CDS             13704..15110
                     /gene="atpD"
                     /locus_tag="IW656_001735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0837444.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15124..15546
                     /locus_tag="IW656_001736"
     CDS             15124..15546
                     /locus_tag="IW656_001736"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0837445.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16015..16245
                     /locus_tag="IW656_001737"
     CDS             16015..16245
                     /locus_tag="IW656_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0837446.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16342..17643
                     /gene="murA"
                     /locus_tag="IW656_001738"
     CDS             16342..17643
                     /gene="murA"
                     /locus_tag="IW656_001738"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0837447.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17656..17832
                     /locus_tag="IW656_001739"
     CDS             17656..17832
                     /locus_tag="IW656_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0837448.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17967..18263
                     /gene="yidD"
                     /locus_tag="IW656_001740"
     CDS             17967..18263
                     /gene="yidD"
                     /locus_tag="IW656_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0837449.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18327..18710
                     /locus_tag="IW656_001741"
     CDS             18327..18710
                     /locus_tag="IW656_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0837450.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18879..19763
                     /locus_tag="IW656_001742"
     CDS             18879..19763
                     /locus_tag="IW656_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0837451.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19880..20113
                     /locus_tag="IW656_001743"
     CDS             19880..20113
                     /locus_tag="IW656_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0837452.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         6737 a         3537 c         4404 g         5583 t
ORIGIN      
        1 gtttttttgc aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca
       61 tccttgaaaa aggaaagcta acaataacta gcatcatata agagctttta aggatagaac
      121 agtagaatag gaatctatgg gcaagtcctc tcgtataaat aaaataggag ggaagtagca
      181 gcatattttt cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa
      241 aattcgagca cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa
      301 agaagaaaaa atcgcgattt tacaagagat tatccgaatt aaatcagtga atggaaacga
      361 aggtgaagta gcagcctatt tgaataagct tttggcaagg catgacataa caggagagat
      421 tgtttcttac agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg
      481 aaaagtgtta ggcctatctg gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg
      541 gacctatgca ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac
      601 agatatgaag agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg
      661 aaaaccattt aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact
      721 aggtggggaa cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg
      781 agagccaacc aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac
      841 ctcgcacgga aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca
      901 cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga
      961 gaatcctgtc ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt
     1021 caacagcatc ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc
     1081 aaatgataaa atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga
     1141 ctatcatttg gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc
     1201 tcctttgatt cattccattc agcaacaatt cagccagcca ttacctttag ttggagcggc
     1261 agcaacaacg gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt
     1321 tgggcctggt gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta
     1381 tttggatatg atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt
     1441 taataaatga aaaaacagag ccagattagc attattttgc taatccagct ctgtttattc
     1501 agcctatttt tctttttgaa tatatcaagc gttggggcaa catagtgatg actaatgaaa
     1561 caacgacgat agacaacaag cgatcaaggt agtctgtacc cacctgtacc aagatcacac
     1621 ttgttgtctg acttaaacct aacccatgta atagctgaac gatcatactg gatcctgagg
     1681 aggtgatccc gccaaataac tgaacggtaa tgatcgaaga aacgatcgtt ccaggtaccg
     1741 acaaaatcaa agcgagtaat aacagtttca agctgttttt atctttgccg agatatcttg
     1801 gcaaaatcaa tccagcaagc aagcctgtga gaatttggat tggcgaataa tacagagaaa
     1861 aaatatctgt agtcactcca cttaaaatac cgctggacaa tcctgtcagc attccaggta
     1921 caggcccaag caatgcacca gcaaaaatcg ttccgataga atcgagatag attggcaaac
     1981 gaagccataa agcaatcgtt cctccaatat aattgatccc aatacacaaa gccatcactg
     2041 taagtacgcg agttgccatt ttgtttttat tcattttgtt agcccatctt taacctttcc
     2101 aaatctttca taatcgtttc ttcttgtgca tttaaaagaa cggttaaaaa ctttcggaaa
     2161 aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg ccaaaattca
     2221 taacgatcaa ccaatgtttg tccgcgactt attccttgag tttctacagc agtgaagctt
     2281 ttaaagcctt cacagatgga ctcatcgata aaataagcaa cagctaatgg atcattgatg
     2341 acacatccta agattctttc ttgtttccag tgaaagtcaa aataaaaacg agtgatcgct
     2401 tttagatact ctccctcttc aggattcatt tggcagcaat attcaaggat agtcggtgtc
     2461 aagacgattt ctcttgtcac atctaaccca accatttcta tcggttgttt gagcttttcg
     2521 aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac tggtgaacag
     2581 ttaccatggc ttttgtaagt tccgcccatt gagacaaaac gatccatatg tttcccaaca
     2641 gatgggctgt ttttcaaagc agaagcgata ttggtcaaag gccccaaagc aatgactgat
     2701 gtatctgttt tttctttgaa ataatcagtc agaaaatcaa ctgcatggag cgattctgcc
     2761 tgtttggcta agaccatcgg gaaatttgtg tcccctaatc catccaaccc atgagtgtct
     2821 tgcgcactaa tgaaaggttg cttcaaaggt ttttccattc cttgataaac agggatatct
     2881 aaacgatcac agcgctccaa acatttcagc acattctgcg taccgatatc tacagggaca
     2941 ttcccgcaaa cgacggtaat agcaactact tcgatatcag gggatttgac agctagtgat
     3001 aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttactttacg cattatttcc
     3061 aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga accagtccaa
     3121 gacgagtgtc ttgtttataa tgcacgaaat tagtatactg gcttttattg tgaatgtaaa
     3181 tgctctctta caagtaaatc tattgagaag aactttaagt aaatgataca atgaaaaaga
     3241 aatggaattt acgtgtgccg aagaaaaaga atcctattga tcggtatatt gtataataaa
     3301 tagattatag tttggcagaa agcggcgggt gtacgatggt tattttttat ttcattctta
     3361 ttctgatatt tataggaatc agttaccatt ttctttctta tttttatgca aagaaattag
     3421 taagagcggg ggtacagaga ggctatgact ggtatgaaga aactgggcac cgcttgatgg
     3481 atcctgctgc agtcacgtca gtagaaaaaa agcgacaaaa attagaagaa gagtgtgaaa
     3541 gcttctggga acaaggaata ccgaaaacga tcaaaagtta tgacgggttg aaactagctg
     3601 gacagatgtt cctccaacca actcagcaga ataaatgggt catttgtgta catgattatc
     3661 gaagtacagg gaaaagagat atgtctcata taggtaaaag atatgcagag aaaggattca
     3721 acgttcttat acccgattta cgtgcgcatg gagaaagtga aggagaaatc atcggaatgg
     3781 gatggctaga ccggctagat ttaattgcat ggatccaact catcttagat gaacaaccag
     3841 atgcatccat tattttgcac ggcagttcga tgggcgcttc tactattatg atggcaagtg
     3901 gtgaaaagct tccatctgca gtcaaaggat ttatcttaga cagcggatat gtttctgttt
     3961 atgcggaatt tcggtatatg ttaagtaaaa tcacggtctt tcctaaaaaa atgatcatga
     4021 gatatgcgaa tcactatgct cagaaatacg caggttattc tttaaagcaa gcatcagcta
     4081 caagacagct tggtagtaac catttgcctt tgttagtcat ccatggtgaa agagatcatt
     4141 ttgttcctac agaagcagca tacacgattc aaaacgctac ggcaggggat aaagcattac
     4201 tgcttgttcc agaagcagaa catttggaag catcgatgaa agatccgaaa acctattgga
     4261 cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttgattttct cttccttttt
     4321 gtaagcaaca cgaggataaa taattgttta tagaccatga gactgttaaa ataaggtagg
     4381 aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga ggaaaaaaat
     4441 cgtcttgctt tattcaatga tgagcaacaa gaaatcggag aaatgacatg gtcggatgcg
     4501 gggccagaca tcatgatcat tgaccataca tttgtcgatc cagcatatcg tggtcaaaaa
     4561 ttagctgaaa agctagtgta tactggagta gaactagcaa gacgagaggg gaaaaagatt
     4621 atcccttcat gcccttacgc aaaaaaagag tttgagaaga aaccagagta tcatgacgtc
     4681 ttacgccaat cataataata acagctgaaa gatttgcact ttcagctgtt tttccattta
     4741 tatagagttg tcacggagtt ttatctatga taaagttaaa gagacgatat cttttgggaa
     4801 ggaggctgct atatgtataa tttaattttg ggaatcgtga tcgttttatc gattgttatg
     4861 gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc atttaccggc
     4921 ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt catgcagcga
     4981 tcaactgcag tcatgggagc tgtctggatg atcttattat ttgtcctagc atttttatct
     5041 tcgaagtagt cgaataagcc tctcgcacaa cggcgggcgg ctttttttat tgcaaagaga
     5101 acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga ggtgaaacga
     5161 gtgaaacaat taccaaaacc cctttatgca aaacatggaa aacgagcagt tttgctgctt
     5221 catgcgtact caggaagtcc aaatgatgtc cggatgctcg cacggttttt agaaaaagca
     5281 gattatacag tctatgcgcc gctatttaaa ggacatggaa cgatggagcc atatgatatc
     5341 ttgcaagaaa aagcagaaag ctggtgggca gatactaaaa aagctatcca ttttttacag
     5401 tctgaacagt tttctgatat tgccgtattg ggattatcga tgggtggcat ctttgctgta
     5461 cgggcactag aagaagaatc agtaattggc ggcggttttt tctgttcgcc cctgtctcct
     5521 gtagaaacaa acgtgcctga aaattttgaa aaatatgtac gtcaagtgct gaagatagca
     5581 ggcaaatcag aaaaagaaat caatgaaaaa gctgtgtctt accgctcgtt agctgaacat
     5641 caattaatgg atatacaaga ccaagcagca atcgcagaaa gtagactatc agatatccaa
     5701 cagccaatat ttttagctca agctgggaaa gatgaaatga tcgacccaaa tggtgtattt
     5761 gaaacggcac gcaaattgag ccgccaacga gtgacacttc aatggtatcc tgaaagcggt
     5821 catgtgatta cagttggcac agcaagacgc gaattagaaa aagacgtgtt ggaattttta
     5881 gaaaaattac cttggaatga ggaataaata tgacaaaaga aacaataaaa gagaaaattc
     5941 tctttttcat ggaaagcagt cgcaaacgta gtttttctat ggaagaaatc gctgaaggtc
     6001 taggctttca aaaaagcgat gattttaaat tactagtaca aacgattgct caaatggaaa
     6061 gagaacagag tatcgttttc aataaaaaag gaaaagtcaa acttccttta aaaccgatct
     6121 tggtcgaagg gacatttcgg gcaaatgaac gaggatttgg atttgtaacg attgatcccg
     6181 aagaagatga tatctatatc ccgaaagaag cgactggctt tgcaatggat ggggataccg
     6241 tagcaattga tatcatgaag cgagcaaata cagatatgga tcgcggagca gagggaaaag
     6301 tagtggagat tcgagaccga gcgactactc agttagccgg agaatttgtt gcttattcag
     6361 acgaagagat acaggaaacg gatctttatg gtgtggtcat cccgaaagat agaaagctta
     6421 accaattcaa agtgtatgca gcggcagagg gagttcgtcc agttgacgga agtattgtat
     6481 tgatcgaact gacccattac cctgaaaaag ggtatgcaac tagtttagaa gggataatca
     6541 agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcgatt gtcgtggcta
     6601 acggtattcc tacaaaattt cctgatgatg tgttatctga ggcagatgaa gttccagacc
     6661 agatcagcga aaaggatatt gttggacgca gagatttaag agatcagctc atcgttacga
     6721 ttgatggaga agatgcaaaa gatctagatg atgcagtgac ggtcaaaaaa ttagaaaatg
     6781 gtaattattt tcttggagta cacattgcag atgtatctta ctatgtgacg caaggcagtc
     6841 aactggacag agaagcatac gaaagaggaa caagtgtcta tttgaccgat cgagtgattc
     6901 cgatgatccc acaacgttta tccaatggta tctgttcatt gaatccccat gttccacgac
     6961 tcaccatgag ttgtgaaatg gagatcgatc caaatggaca agttgtctct catgagattt
     7021 ttccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat caaatcttag
     7081 aagatcaaga tgaagaaacg atgcaacgtt atgctcagct cgtgcctatg ttccaagaaa
     7141 tgggtgaatt gcatcgaatc cttgaaatga tgcgaatcaa acgtggagcg atttcttttg
     7201 aggatcgtga agcgaagatc cttgttgatg ctgaaggaca cccaaatgat atccagctaa
     7261 gaagcagagg gataggtgaa cgcttgatcg aatcctttat gcttgctgcc aacgaaacgg
     7321 tagctgaaca ttataataaa ttaaatcttc cgtttattta tcgaatccat gaacagccaa
     7381 aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt ctggtccgag
     7441 gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga gtagaagaca
     7501 aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa gcacgctatt
     7561 cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac tttacatctc
     7621 ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac agtgaagata
     7681 aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca gatcatagtt
     7741 ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg aagaaagcag
     7801 aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct gttgtgaaat
     7861 tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc aatgaattga
     7921 aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa agaactggtc
     7981 tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat ccagatgaac
     8041 gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa cgtccaaaag
     8101 cacgcaatgg aaaatctgga aaacgagaag gaaaacaagc aggcaaacgc tcagaaaaaa
     8161 atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag aaaaaaggga
     8221 aaaagccatt ttacaaaggt gtcaaaaaga agaaaaagaa aaaataaaat gagttggagg
     8281 aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa gctcgccatg
     8341 attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg gagatcaagt
     8401 cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc aacggggaag
     8461 ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt aatcatgatc
     8521 cgttgcgcac gagaaagtta ttattacaca agaaacaaat cgttcgtctt gaaaacgaat
     8581 tgaaaaacac tggaatcact gttgttcctt taaaagtcta tattcgtaac ggctatgcca
     8641 aggtattgat tggtctggca aaagggaaaa aatcgtatga taaacgggaa gatttgaaac
     8701 gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga taatttcatt
     8761 atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt gttagcattt
     8821 aatctgataa aacatgatta tcacaggttt tttcttagat caggagcacc gtgcatgggg
     8881 aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg aaaatgtttt
     8941 gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg gaaaaaacca
     9001 gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa tggattggat
     9061 gaacgctcgc taacgtttca cattggacca atttggtttg atggtacagt ttgtataatg
     9121 gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag aaatcttaag
     9181 ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag tcgaggaatc
     9241 gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc atttacattg
     9301 tttttatttg tttttgttgc aaacaacata ggtttagtaa caaaaatcgt cttacctaac
     9361 gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac tttggcattt
     9421 ataatgatct tacttactca cttattcggg gtgaaaaagt taggcttcaa aggatatttc
     9481 gtaaattcgt tcttgaaacc ctatagtttt atgtttccga tgaaactgat tgaagaattt
     9541 acaaacttat taacacttgc tttacgtctt tatgggaata tttatgcggg ggaagttttg
     9601 ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc attagcaatt
     9661 ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca agcatttgtc
     9721 tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga ataatactcg
     9781 gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga attatatcgc
     9841 agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg gtcaagttat
     9901 ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat tccgtacaac
     9961 aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag ttatcgcctt
    10021 gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca tcgtttgtta
    10081 ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc ctatgctgaa
    10141 tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg ttgtcagcgg
    10201 ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa tcagcgatat
    10261 tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac aatctcgcat
    10321 caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc gttctgatgc
    10381 agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa atattttgaa
    10441 ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata tcactgtaga
    10501 acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc ttcaaatcgc
    10561 ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa tcaatcaata
    10621 cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga agacgttacg
    10681 ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc tatcaagatc
    10741 tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg ttaagtgatg
    10801 ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc ggttatgatg
    10861 gaatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat gaccttttgt
    10921 tcatgattga agaatatgaa cgtcgatatg atgaacatca agggttacta ttgggaagtg
    10981 tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag aatgttgcaa
    11041 aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct attattggtg
    11101 gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg caattagaaa
    11161 aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat ggccatcaaa
    11221 gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa cgtgctttca
    11281 gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc acatggttta
    11341 gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg aatggcgcaa
    11401 aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc cattcgtgaa
    11461 ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga ggccttgatc
    11521 ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat cgttactgac
    11581 aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc tgttaacgaa
    11641 ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg cggacaacgt
    11701 gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgattga tacgatcatc
    11761 aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa agattctaca
    11821 gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac aatcgttgtg
    11881 aatgccggtg cgtctcaacc agcaccatta ctttatatcg caccatatgc tggtactgca
    11941 atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga tgatctttca
    12001 aaacaagctg ttgcttatcg tgaactttcc ttgctacttc gccgtccacc aggtcgtgaa
    12061 gcttatcctg gggatgtttt ctacttgcac tcacgtttat tagaacgtgc agcaaaatta
    12121 agtgatgatc taggcggcgg ttcaatgact gctttgccat ttgttgaaac acaagctgga
    12181 gatatctctg cttatatccc aacaaacgtt atctcaatca ctgacggaca aatcttctta
    12241 gaaagcgact tgttctacgc gggtactcgt ccagcggttg atgccggact atctgtctct
    12301 cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac tttacgtcta
    12361 gacttagcaa gttatcgtga attagaagca tttacacaat tcggttctga tttggatgcg
    12421 gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa acaaaaactg
    12481 catgcaccgc tagctgttga gaaacaagta gtgattttgt atgccttgac acatggattc
    12541 ttggacagta tccctgtaga cagtatttta gacttcgaac atgaattatt cgaatatctg
    12601 gataccaatc attcagatat ttttgaaaca atccgtacaa caaaagatct tccagaagaa
    12661 gaaacgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac aaaaggaaat
    12721 aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg tgagatgaat
    12781 gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa cgagccaaat
    12841 cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag cttcttcaca
    12901 gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga ctgctactca
    12961 gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca gcatgctgct
    13021 cagccgtcct gtgaagaaaa ccggttatat cgtcatcact gcagatggcg gtctggtcgg
    13081 tggatataac agttcgatat tgaaacaaac gatgtctatt ttagaagaag accaccaatc
    13141 acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct ttaaagcacg
    13201 cggtatcaac ctagcatatg agctgcgcaa tttatctgat cagccaagct ttgatgaagt
    13261 ccgcaaaatc gtaaatatgg caacaactat gtaccaaaac gaagtattcg atgaattata
    13321 cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag aaaaaatgct
    13381 gccaatctca gacttagatc cagaagaagc aacgactttc gaacaagaat atatttttga
    13441 accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa gtttgatcta
    13501 cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag caatgaaaac
    13561 agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttata accgtgcaag
    13621 acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg cactagaata
    13681 gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta atcggtcccg
    13741 ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac gcattagttg
    13801 tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta gaattagggg
    13861 acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga ggaatggaag
    13921 tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta ggtcgcgtat
    13981 tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat gcggaaagaa
    14041 gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca gaaattttgg
    14101 aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg aaagtcggac
    14161 tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc cataatatcg
    14221 cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact cgtgaaggta
    14281 atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc atggtgttcg
    14341 gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt ttgacgattg
    14401 cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac aacattttcc
    14461 gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct tctgcggttg
    14521 gttatcaacc aacattggca acagaaatgg gtcaattgca agaacgtatc acatctacga
    14581 aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac tacactgacc
    14641 cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa cgtaaattaa
    14701 cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt gctttagcac
    14761 ctgaaatcgt gggagaagaa cattataaag tagcaacaga agtccaacac gtattgcaac
    14821 gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta tccgatcaag
    14881 aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa aactttaacg
    14941 tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa acagtgaaag
    15001 gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct ttccgaagtg
    15061 ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag aaaggggtgc
    15121 cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt ctatgatcac
    15181 catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt gcctaaacat
    15241 gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac agattcagat
    15301 acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga taatgttgtt
    15361 tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg tgcggaacgt
    15421 gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga tacgaacgaa
    15481 ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt atctaagcac
    15541 tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga ggttatgaca
    15601 gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc aaatttttgt
    15661 gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc agaggttgtg
    15721 aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc tcatattttg
    15781 ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt ggaataaggg
    15841 actgcgacaa gacttttgtc tcagtccctt tttttgcata tttttagcat ttatttccta
    15901 aaaaatgtaa ggggattcct atatttttaa gtatacttgt ttttcctttt atacatttta
    15961 tggtattatc attttattag ttattataaa acaatcgtta gaaaggacgt cattatgcaa
    16021 gtatacggaa ttgatgcggt gcttcgaatc gtgagtcatt tggcgtttat ctacttagcc
    16081 ttctggtctt tacgttcgtt acgcattgaa acttttttca gaccaatgca taatacgcaa
    16141 attagaatgg caatcgtact tttttcaatt tttttagggt acactgcaag taattttttc
    16201 ttagaagtta tcgctttatg ccgaaatctt ttcgttagcc tgtaaatttt aaaaaaaggt
    16261 actattttaa aagcaaggat gttataatag aaaagatttt gagtaagtgt atgctcattg
    16321 gaattttttg gagggaacaa catggaagag atcatcgtaa gaggtggcaa tcaattaaat
    16381 ggaacagtaa gaattgaagg agctaaaaat gctgttttgc ctattttagc tgctagtttg
    16441 ttagcagaag aaggtattac gacattagac aacgttccga ttctttctga tgtcttcact
    16501 atgaatcaag tgattcgtca cttaaacgta gatgtagatt ttgatgaaca aaagaatcaa
    16561 gtaacaatcg acgcttcaag acaattagaa atcgaagcgc cttatgagta tgttagtcaa
    16621 atgcgtgctt caatcgttgt gatgggtccg ctacttgcca gaaatggaca tgcaaaagta
    16681 gctatgcctg gtggatgtgc gattggtaaa cgaccaatcg atttgcattt aaaaggattt
    16741 caagctttag gagcaaaaat tattcaaaaa aacggctata tcgaagcaat tgcggatgaa
    16801 ttgatcggta acacgattta tttagatttc cctagtgtgg gtgctactca aaatatcatg
    16861 atggctgccg taaaagcaaa aggaacaacc atcattgaaa acgtagctcg cgaaccagaa
    16921 atcgttgatt tagcaaacat tttgaataaa atgggcgctc aagtctatgg agctggtact
    16981 gaaacaatgc gtattgaagg cgtggatcat ttacatgcag tcaaccactc aatcgtacaa
    17041 gatcgtatcg aagcaggtac ctttatggtt gctgctgcaa tgacacaagg caatgtgctg
    17101 attgcagatg ctatctctga acataactgc ccattgattt ctaaattgat cgaaatgggt
    17161 gcagaaatca ttgaagaaga aggcggagtc cgtgtcatcg gaccaaaaca tatcttacct
    17221 acagatgtaa aaacgatgcc tcatcctggt ttccctacag atatgcaagc tcaaatgaca
    17281 gctatccaat tagttgcaga aggtacaagt gtggtgactg aaacagtgtt tgaaaatcgt
    17341 ttccaacatt tagaagagat gcgccgtatg aacgctcatg tcaaaatcga cggaaatgta
    17401 gcaatcatgg atggaaacca tgaattgcaa ggtgcagaag tatatgcaac agacttgcgg
    17461 gcagcagcag cattagtttt agctggcttg aaagcaaatg gaatcacacg tgtgcgtaac
    17521 ttgaattatc tagaccgtgg atattataat ttccatatca aactacagca attaggtgca
    17581 gacgtcgaac gtgtagacat ggatcaaact tctgctgaaa aaacagcaca aacgattgct
    17641 taaggaagtg gctgaatgag taaccaacga tatatcttat taactttgat aaagatttta
    17701 gtggtgatct tattgcttat ccttcttttt gtggccggaa cgatgatcgg atatggtgtc
    17761 atcggtggcg gcaatccatt taaagtattc cagccaagtt tgtggattca tattcgagac
    17821 ttttttcatt gataaataag aatcacagtg gttctagtcg atatgaagtg agccatgaag
    17881 cagaacatga taaaagaaaa cagaagggaa cgttgacgtt agtcgcgatc ccttttttta
    17941 gtaaaagaaa agaaggaatc taattgatga agaagatatt gatttattta gttagaggat
    18001 atcagcgctt tatttcgcct gcatttccac ctagctgccg ttattaccca acctgttcca
    18061 attatatgat aaaagcaatt caaacccatg gtgcgctaaa agggacggta atggggactg
    18121 cacggatctt acgctgtcat ccttttgtca aaggcggtat cgattatgta ccattgacat
    18181 tccgattaac gcgaaataag aaagataaaa aagaaccaga ttataaaaag gttgtgaaaa
    18241 atacgggtag cactgagcga taaagaaaaa gaggaaattt attgtcaaag taacaactcc
    18301 gcaactactt atatggggga caaaaaatga tcgaagaact aaacaagaaa gatttaccat
    18361 ggtccttgtt attagatgca gatcctgata aagaaaaagt tcaagtatat gtttccagag
    18421 ggagtggctg gatatggaaa gaaaaagaaa aaacaatagg tgtactgatc tatgtggcaa
    18481 gggaaaaaga atacgaaatc gtcaatgtag cagtaacccc aacgtatcaa ggaaagggga
    18541 ttggcggaaa attacttgag acagcttttc agaagctttc acagctagct tcaaaccaaa
    18601 ctcggatcat tatacgtact ggcactacta gtagcgctgc gttacacctc tatcaaaaaa
    18661 tgggctatgt ggagattggt cgagtcaaag actattttat ccatcattaa tctgagccca
    18721 tttttgaaaa tggagatcaa ttaagggatc aagtgatttt agcaattcac ttacctaaaa
    18781 atagctttaa ctaataaact ttcaattcga gaaaatgctt tcaaatgcta aatagataca
    18841 ttacaatagc tttagcaaac aaaaaggaga atatttttat gggaatatta gtttttgatt
    18901 ttggtggatc ggctgtcaaa tatggttgtt gggatggcaa agagattaaa ggtaagggac
    18961 aatttgcaac gcctgaatct tgggaagaaa tgaaaacgca attatttcaa gtttataaaa
    19021 aaatactcgt ttcttttgaa gtcgaaggaa ttggaatcag ctctcctgga gtagtgaata
    19081 atgagaaaca ggtgattgag ggaatcagtg cgattcctta tattcatgga tttaatattt
    19141 tccgagattt ggaaactctg tttcaactcc cagtaacgat tgaaaatgac gccaactgtg
    19201 ccggaatggc tgaattttat gaaggtgctg cccgtgatta tcaagatgtt gcttttgttg
    19261 tggcaggaac tgggattggc ggggcgttgt tcacgaaagg acaaataaat aaaggggcac
    19321 atctttacgg tggagaattt gggttgatgt tcttagatgg cgataagaca ttcagtaaac
    19381 ttggtacagc ggtacagatg gcttggcgtt attgtgaacg caaaggtctc gataaaaatg
    19441 cttataccgg aaaagatgtc tttgaattag cagaaactgg agatgcaata gcaaaagaag
    19501 aagttgagag tttttatacg tatcttacaa agggattatt cagtattcaa ttttcgtttg
    19561 atccagaagt gatcgtatta ggcggcgggg tttctgcaaa agaagggctc attgatgaga
    19621 tcaaaagccg aatgaagaag ctgacagaac aattcgatct gcatgatttc gaaccgcaga
    19681 ttttattatg tgagtacaga aacgatgcta atcttgtagg tgcagcagca aactatctcg
    19741 ctgtacgtca aggagagaat taagagcttt agagaaggtg cagcttgcat aaaagaagag
    19801 aatgaaatga ataggagatt tttacctaag gaatgtttta tttgctgcag tatgataaaa
    19861 tatctgagag gtgaaaatga tgtcaaaaaa aaataaagat attgaagtgc gtatagaaga
    19921 agtgaaaaaa gatatcaaag gtaagaatta tgaagtcact cagttattta tagggaaaaa
    19981 aatgattggt gagatcttag cctatgggcc aaaagaatat gaaattttct ttggtgaaga
    20041 agattttgga aaagaaaaaa gtcttgaaaa tgcgatagaa acagtgattc gacactggaa
    20101 tctgcacgaa taaaaaaata tcaaaaaaac tttcaataaa gggttgcaaa aatctggaaa
    20161 attaggtatt ataactaagt caggttgata caaacctttc ggaggagtag cgaagtggct
    20221 aaacgcgacg gactgtaaat ccgttcctta gggttcagtg g
//
LOCUS       DACEJZ010000053        19925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEJZ010000053 DACEJZ010000000
VERSION     DACEJZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.055"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(122..1489)
                     /gene="nhaC"
                     /locus_tag="IW656_001744"
     CDS             complement(122..1489)
                     /gene="nhaC"
                     /locus_tag="IW656_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0837453.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1598..3019)
                     /locus_tag="IW656_001745"
     CDS             complement(1598..3019)
                     /locus_tag="IW656_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0837454.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3262..5139)
                     /gene="tdc"
                     /locus_tag="IW656_001746"
     CDS             complement(3262..5139)
                     /gene="tdc"
                     /locus_tag="IW656_001746"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0837455.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5430..6686)
                     /locus_tag="IW656_001747"
     CDS             complement(5430..6686)
                     /locus_tag="IW656_001747"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0837456.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7266..8567
                     /locus_tag="IW656_001748"
     CDS             7266..8567
                     /locus_tag="IW656_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0837457.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8636..9235)
                     /locus_tag="IW656_001749"
     CDS             complement(8636..9235)
                     /locus_tag="IW656_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0837458.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9489..10370
                     /locus_tag="IW656_001750"
     CDS             9489..10370
                     /locus_tag="IW656_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837459.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10383..11993
                     /locus_tag="IW656_001751"
     CDS             10383..11993
                     /locus_tag="IW656_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0837460.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12070..12729)
                     /locus_tag="IW656_001752"
     CDS             complement(12070..12729)
                     /locus_tag="IW656_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0837461.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13012..13677)
                     /locus_tag="IW656_001753"
     CDS             complement(13012..13677)
                     /locus_tag="IW656_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837462.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13824..14429)
                     /gene="dhaL"
                     /locus_tag="IW656_001754"
     CDS             complement(13824..14429)
                     /gene="dhaL"
                     /locus_tag="IW656_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0837463.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14457..15440)
                     /gene="dhaK"
                     /locus_tag="IW656_001755"
     CDS             complement(14457..15440)
                     /gene="dhaK"
                     /locus_tag="IW656_001755"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0837464.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15442..15837)
                     /gene="dhaM"
                     /locus_tag="IW656_001756"
     CDS             complement(15442..15837)
                     /gene="dhaM"
                     /locus_tag="IW656_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0837465.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(15927..16886)
                     /locus_tag="IW656_001757"
     CDS             complement(15927..16886)
                     /locus_tag="IW656_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0837466.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            17056..17376
                     /locus_tag="IW656_001758"
     CDS             17056..17376
                     /locus_tag="IW656_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0837467.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(17488..18357)
                     /locus_tag="IW656_001759"
     CDS             complement(17488..18357)
                     /locus_tag="IW656_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0837468.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(18453..19757)
                     /locus_tag="IW656_001760"
     CDS             complement(18453..19757)
                     /locus_tag="IW656_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0837469.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         5672 a         4119 c         3638 g         6496 t
ORIGIN      
        1 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc
       61 ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc
      121 ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg
      181 caagaatcga gaaaaaggcg aatggcaagt aatgcaacgc gctaactttc atcgttccca
      241 taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg
      301 ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg
      361 tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg attccgatac
      421 agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt
      481 gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta
      541 aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta
      601 gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga
      661 ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta
      721 atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca
      781 atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag
      841 ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga
      901 atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa
      961 tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac
     1021 cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca
     1081 aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa
     1141 ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat
     1201 aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca
     1261 aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga
     1321 tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa
     1381 ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca
     1441 acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc
     1501 tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta
     1561 aatcagcgac aaaacgtcgg ctgaaagact tattttttta tttttcttct aaactttttt
     1621 ctacctcaat cccccaagaa tctttcttga agtgattgat gatcaatgga ataacgaaca
     1681 tgaccactgt gatagctgct actaataatg catcactcaa tgtcgctgta cctaatgtag
     1741 caaatacgac aacgatgata gagaagatca gcatgcagct gactacccaa gctaatccgt
     1801 ttccgttttt accaattcgg taaggacgtt cgaattctgg atgtttctta cgcattttaa
     1861 gcaatgaaac agcaatcaaa atgtacacga tacaataaat aacagttgta gcatttgtaa
     1921 gtgtcaagaa tacagcactt acgtcatcca ttacaccata aagcaaagca aataatgaaa
     1981 taacgattga ttgtgttaga acgacatttc tagatacgcc gtatttgttt tctttatgga
     2041 atccgaattt tggtggaagg aatccttcac gagctacttg gatgattgtt tttgatggtc
     2101 cagtaaccca agctgataat tgtaaaagca caccaataaa taccatgaag ctgaagatat
     2161 ttccgatcac ttctggtaag cctaggattt gacagtcaat caagataggc tgcgtgatat
     2221 ttgctaattc catttttcca tctggtacga catttgctac aagcatcgca ttgatgacat
     2281 tcaataaaac taatccgatc aaagcgataa atacgccttt tgtataattt tttgttgcgt
     2341 ctttcaaacg aggcatgtaa acagaactca tttcgatccc aacaaaaata aatgtgatac
     2401 cagctaagta tttcaatgaa tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa
     2461 agtcacctag atagccgttt gagactagtc cgactttgaa ggctgccaaa acacctaaaa
     2521 cgaacatgat gactactggg atatataccc ctagccatac accgatgttc ccgccgactt
     2581 ttgccatatc gaatttcaag ttcaagatcg tgatgatcca gtagataact aaaatacaac
     2641 ctaaaacgaa ccagtgatta ttgccaagtt ctacattacc aaatgtgttt cctaacaatg
     2701 gtcctaaagt agaagcgacc attaccatac ctgggaacat ttgcacccat aatagccatg
     2761 caacgacaaa tccccatttg ctgcccagtg ctgtttttac ccataactga ggaccgcctt
     2821 cttgaggaag catcgtggaa agctcaccgg aaaccattga tatcggaccg gcaaaaaaca
     2881 aaactgcaaa aattgaataa aaaatcaatg tccaaccgac tgcggctaac gtaggaatac
     2941 tccgaaccgt tgcacacagt gccattgtca gaccgataaa ggtcccaaga cccattttac
     3001 ttacttcaga tttactcacc gttgacactc tccttgattt tttggataaa ataacggtgt
     3061 ctttgaagta gtttcagcaa taaacgaaac aattcagcga atatttgagg ggattctttg
     3121 agtttattgc ttgaagggtt tcttcaaaga caacctcaaa acaaagggat cgaagagctt
     3181 aggtctcccc ttgctcttca gatccaaaca tgcgtcagaa acagttagta ttcaagctgt
     3241 tttctaacat gattaccttg cttattttgc ttcgcttgcc aataattggt ctgcgtagat
     3301 tttttccaat ttttcttgaa gagctgcttt gattttttca gcatattctt caaagtgttc
     3361 ttctttgttc atgtatggtg tcattacgct ggcacgcaat acagttactt ttccggcacg
     3421 gttccattct tcatctgaga atcctagttg gttcacaaat tgtaatgggc tgttgccata
     3481 gtctgggata gcaaagtcag tatgtgaagt taagaactca ttaccgtaaa tgcttccttt
     3541 cacgtatgaa gaataatcat atacgtcatg gttcaattta ttcattgcaa ctaagtcatc
     3601 gttgcctttt tctttgaata catagtctac catattgaaa tctggataag ttaatggatg
     3661 aacttcgatt tctttgtctc ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg
     3721 atgtgcacct tcgattgatg cacccattaa tttaccatat cctgtgacat tcaaaggtaa
     3781 gacatggtgt gctgcccaaa cgctggctgc tgttgcccct gcttttgaac cttccaagat
     3841 ataagcccca agtaatgctg ggatatctgc gcctttttca aatacatatg tcgcaaagta
     3901 agagataacg tctctcatgc ggatatcttt gatgacgata ccaccggcag aatatggaac
     3961 atagcccatt ttatgtgggt caatcgttac agattctgct tcttcgattg ctttatatgc
     4021 actgtgtact tcttctaaga tataatcttt gttttctgta aagacattgt atttgtaatg
     4081 aacatctttt aaatcttcaa atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg
     4141 gccgtatccg ccataagcag catctacgtg taagtagaag taaataccgt ctttttctaa
     4201 tacgcgacgc aatgcaacga ttttatcgat accgtcgatt gctccttctt ctgttgatcc
     4261 tacaacaccg actacaccta ggattggtgt tttttcagct gctagaccac gaacgatttt
     4321 ttcaagttcg ttgatgtcca tgcgatagtt gtggtcaact ggcactggaa tcacttggtc
     4381 taaaccgata ccgatgatgt cggcagcttt tagccatgaa tagtgtttag tttgtggaac
     4441 caaccatttt cctaattttt ctaaatgttt tccgctacgt gctgagtgag ctttgatttc
     4501 gtcgattttt tctgggacgc tgtctaacag attcatgatt tcttctgttg acaagttcat
     4561 taattcccaa tcgctttttc cagcaactag ttcaggtgtc acttctttca ttgcaagcgg
     4621 caatgatttg atgttgcggg cataccaaag tccttctaag tttgctaaag aaccatcagc
     4681 aacgatgtgg ccccagccat ctttatagct cattaatttt gcaaattcca ttcctacttc
     4741 ttcttccatt tggcttgttg caggagaaga ttcataggca acgttattac cattccatag
     4801 catagcaaaa ttgtacgcta gtaaagatgg catcaatgtt tcagagttca tatgacccca
     4861 gtaacgtccg gcattatgcc aaggaacgga atgggtacgc atccgcgctg aaatttctga
     4921 tagaacatcc tttgttttat tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg
     4981 cgtaataata ggcatgtctt gaggcatgta gttttgacgc cagcctaaat gttcatccac
     5041 caattcattc aacaaagctt tatagatttg gccgttctca gctttgtccc caatgaatag
     5101 ggcatttagg tttagatctt tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa
     5161 tatttaaatt ttgtcgagcc ctacattaca cctgttcttc tattctgtaa actaagtttt
     5221 ggcctgattt ttaatcatat tttaatatgt atcaattatc cgaattgttc acacttttga
     5281 aataattaaa aaaaacgagg gaatttacaa atgtaaactc cctcgcaaag attgtattaa
     5341 cgacaaccag cgttctattt taggatgggt ttcaaaattg gaatgttgta atagtatttc
     5401 cctttttcaa ggtaaaaact tactaaattc taatcaatta ctttggccaa aaagtaattt
     5461 ttcttacctt ttcgaataac cacaaactta ccatcgaatt catcggatgg atttatgaca
     5521 aaatttaaat cggtaacgcg gtcaccgtta atacttatag ctccgtttga aacatcttca
     5581 cgtgcttgac gtttagatgg ttcaattttt gtggaaacaa gcagttccac gatattttca
     5641 ggtgtgcttg aaatttcaac gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca
     5701 tttagttctt taatatttcc agagaacaat gcttgcgtta ttttttgcgc ttctttcaag
     5761 tcttcttcac tatgcacaaa tcttgtaact tcttctgcca aacggcgttg tgcttcacgt
     5821 ttctcaggtt ctgtctctac ttttttcgct aagtcttcga tttcttcttg agacaagaaa
     5881 gtaaagaatt tcaagtattt cactacatcg cgatcatctt gattcaacca gaattggtag
     5941 aactcaaatg gtgaagtttt cttcggatca agccaaacgg caccgcctgc agtttttccg
     6001 aatttcgttc cgtcagcctt cagcatcaat gggatcgtta aaccgaacgc ttctgcttct
     6061 ggtccttcta attttcggat taggtctaat ccagctgtga tatttcccca ttggtctgct
     6121 ccaccgattt gcaattgcac atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc
     6181 aggatttggt aagtaaactc tgtaaatgag attcctactt ccaaacggct ggcaacgata
     6241 tcttttgcta acatcgtgtt gatgttgaag tttttaccgt agtctctcaa aaattcaagt
     6301 aaagagattt ttgacaacca gtcgtagttg ttcacaaatg tgatatttgc atctttacca
     6361 aacaattttc tcatttgatt ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt
     6421 tgcaacacac gttctgtttt gcggccgctt gggtcgccga tcgttcctgt tccgccgcca
     6481 atcagaatat atggatgatg gcctgccaat tggaatcttt tcatcatcat aaatggaatc
     6541 aaatgtccga tatgcatcga atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc
     6601 ttttcttcaa cgagctcgcg aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc
     6661 caagttaatt catcaataat attcatttta ctgacctccg tgtagttttt ttatgaaagc
     6721 aggaacctgc tagcatgtgt aattaattat ttcttcagga taggatagga aataaaaaaa
     6781 gtcccttggc atataagcca agggacgaaa tctcgcggta ccacccaatt tgtatgtcta
     6841 gatatccaac atacctctcg aacatttaac gctgtcgcgt ggcaatcact gccaaagact
     6901 cctgaaatgt aattcgcttt aaagcatgta ctggtttgca gcgaccacca gctttctgaa
     6961 acattggctt taacaactac ttcgtttcga tcttcgtctt gttattatta atatgtgtct
     7021 atcatagagc gcaacttttt ctttgtcaac agaaaaagga tatttttttt aaattcctca
     7081 ttttttcttt tttgaatccg ctctaattcc gaacatttta ttttcaatta ttgttaaaag
     7141 aaataacttt ttctgtattt tttctgcaat ttatcgagaa gtcactttac tttgcctctt
     7201 attctttctt cgttacaata aggaatggga caagtaaaaa aatgaaaaag caagggacga
     7261 ggtatatgga aaaaaagcaa aaaattattt taggagccac ttctgcttta actatcgtta
     7321 ttttaattct aagtttgttt tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc
     7381 ctgaaaaaaa aataacagaa aaagcaaaag aaccgacaga tgataagcaa caactagtta
     7441 ctcaagcaaa aaatctggct caaagctacg attatgacca agcgattgcg ttattggaaa
     7501 aagatagtaa gaacggatct actgaaacac aacagcttct tcaaacacta aaaaaggaga
     7561 aagaacaact gatcgactgg tctgatccta ctcagatctc tcatgtcttt ttccacagct
     7621 tgatcgtgga cccgcaaaaa gccttccact ctcaacaggc acaaggatac aaagattata
     7681 tggtgactgt tgaagaattc aaccgttcga tcgaacaact ttatcaaaat gattttgttt
     7741 tagttaattt gaatgatttg atccaaaaag acgaacaagg aaatctttcc tttaacgggc
     7801 ttgcgctacc tgaaggaaaa aaaccattgg ttctttcaca agatgatgtc agctattatg
     7861 aatatatgga taattccggt tttcctagcc gattggtcgt atcaaataac aatgaaatca
     7921 aaaatgtcta taaagagaaa gataaagaag aaatcggtga ctttgacatg gttcctctga
     7981 tcgatgcctt tgtcaagaaa catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg
     8041 ccctgacagg ttacaacgga gttttaggtt atcgaacatc aaaatctcaa tatggagaca
     8101 atgaaaaaac aaaaaaagag attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag
     8161 acggctggag ctttgcttcc catacatggg gtcacttgaa tatgactcaa gcttcactag
     8221 aagatgtaaa aaaagacaat gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa
     8281 caaatattct gatttatcca tttggtgcag acatcagcga ctggcagcct tattcagcag
     8341 aaaatcaaaa atttgcttac ttgaagagtc aaggtttcga tattttctgt aatgtcgatg
     8401 cttctacacc agcttgggga caattaggta cggatttcta tcgaaatgct cggatcaata
     8461 tcgatggcat tcgttttcaa tctgatttag acggaaaaaa tcctattctt gatccattca
     8521 tcaatgtcca tgatgtctat gacaaagaag cacgcgaatc tgcttgattt tacgtcattt
     8581 atgaaccaaa aaagccgaac tgacatttta tcgtcagttt cggctttttt atgctttatt
     8641 tcttcttcgg tgcgattccc ttgatcagga cttctgtcca ttctgctgta tatggaagca
     8701 gttttgcgta tatctccggc ttagaaattc cttctaccat tgcttgaaaa tagaggatca
     8761 agaactcatc cgaatatttc aaatccactt ttccttcttt ccgccctcga tggaaaagat
     8821 caagcatgat actgaaactt tcttgcgtat acgcatccat cgcgtcagaa aattcttttt
     8881 tttctttttt tgtatagtat gtcatcaaat cgatataaaa ttgtttgttc actcgattga
     8941 gataacggat tttgttttca ctcatggcaa tcagtgtttc ttcaaatgga agattttccg
     9001 caatcaacgc tttcgcttct tgccctaaat cattcataaa tgcaaaaaat acttcataaa
     9061 tcagcccttc cttgctgcca aaatatttaa aaatcgtcgc tttacttacc tgagcgttct
     9121 cagctacctt atccattgtc actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag
     9181 ctgcttgtaa aatctctttc tttttctctt gtgctctctt ttcaaaaccg ttcatttgta
     9241 gagcctcccg taattttaaa cttttataat atatatagtt caaatttatt ctgaggttta
     9301 ttataactat ctattgagaa agtatcaagc tagcaattcc ttgtttatga attcattgac
     9361 agaaatttat aaaaagaata ttatgtacta aaataataaa aaaagtttta taaaaaggta
     9421 taaaaccttt ttattaaact atttaatctt gtttagtata tatttttaga aaaaggagtg
     9481 gggtttttat ggcagaaatc gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg
     9541 aagctttaaa agatgtctct ttcaccgtac attcaggtga agtcgttgga tttatcggac
     9601 caaatggttc tggaaaatcc acgacgattc ggacactttt agggattata aaaaaagacg
     9661 gcggaacggc aacgattttt gggcaagatg tatgggaaga tagtttatct atccatgaac
     9721 gtctttctta tgttccaggc gatatctctt tgtggggaaa tttgactgga ggagcgatca
     9781 tcgatctctt catgaagctt catggaaaag ggagtgctca aaaaaaagat gaactgattc
     9841 aacgtttctc tcttgatcct aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa
     9901 aagtgggctt gattgctgct ttggctgttg attcggatct ctatttattt gatgagccta
     9961 cttctggttt agatcctttg atggaaacga ttttccaaga agaagttgaa aaattgaaag
    10021 aacagggcaa atccatccta ctttcttctc atattctcag tgaagtcgag cgactagcgg
    10081 ataaagtcgt gatcatacgc gaaggaacac tcgatgaaga aggaacactc gatgaattgc
    10141 gtcatttgac tcgttcgacc atcactatag aaacagaagc agagaatagt cacttagcac
    10201 aattgaacgg tgtccatgac tttgtgcaaa aaggtcacct cgctcaattt gctgctgata
    10261 acgaagcttt cgatgtgatt ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat
    10321 ctattccacc gactcttgag gatttattca tgagacatta tgaaggatga ggaggaagcg
    10381 aaatgactga taaattttca cgctggtcca cccttttttc tcaaaacctt agccgtgact
    10441 ggaaaaagat cctgatatgg ctagtcggtg tcactgcttt ttctggtggt tttgtccctg
    10501 cgtttgaaga attgacttcc ggacaaggat tagctggatt atacgaaacc ttgcaaaacc
    10561 cggcaatgat tgcaatggtc ggaccaactc cagcttcgaa tgctgctgag tatactttag
    10621 gagcgatgta tgcaaatgaa atgttgcttt tttgcagttt gtttgctgct gtgatctctg
    10681 ttttacacgt ggtgagccaa acgagaaaaa aagaagaact tggtctgatt gaacttattc
    10741 gttcctttcg agtcggacgt caagcaaact ctttagctgt catcgctgaa agtattctga
    10801 tcaatttgct tttagcaata tcgatcagct gtacgatgat ttttttccaa gcggattcga
    10861 tcactgcaga aggtgcttgg ctttttgggc tatctgtagg tagtatgggg attttgggga
    10921 gtgccgttgc actgtttgct gcacaactga tgccaagttc agccagttcg aatggtctgt
    10981 ctttagcgtt tttaggtgga ctttaccttc tacgagcaat aacagatgta gctgattcct
    11041 ccctttcgat gtggaatcct ttaggctggt cttatttgac ctatccattt acaactaaca
    11101 actggattcc tttgctttat ttgtttttgt tcagtctaat cttgataatc agttcatttg
    11161 ttttagagga acatcgagat ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt
    11221 atgctaaacg ttctctttta tccttgcgtg gtttattatt ctatctaaat aaagggatga
    11281 caatcagttg gcttatcact tttggtttga tgggggcggc atacggctct atctacggga
    11341 atatgcaaca gtttttagaa agtaacgact taatgaaaca gatgtttatc caatctggcg
    11401 tctcaattga aaaaaacttt acaagcacga tcatggtcgt cttgatttgt ctagctgcta
    11461 ttttaccaat tgctagcatc aatcgtttat ttacagaaga aacccacgga cgtatgaatc
    11521 agctgttcgc aacaaaaatt tctaggaacc aactttattg gaccaatatg ttactagcta
    11581 ttctatttgc tatattggca atcctgatta gttctggtgc actagggctt tctgctttgg
    11641 cttcgatgaa ggatgcgaaa gacatggatc tattgatgtt tcttaaagcc gggttcaatt
    11701 tcctgcctgc cgttttagtg tttatcggaa ttagtggctt tctattcgga tggcttcctc
    11761 gaatcggtaa acttgcttat atctacttag cttactcctt tgcactcaat tactttggcg
    11821 gaattttgga tttaccagaa tggttttcca aaacagctgt ccctagctgg gttccacgga
    11881 tgccaaacga ttcttttgat ctttccgtgt tccttacgtt atcatgtatc agccttgcac
    11941 tgttcgtcct tggttctatt ggttataggc gaagagactt actagaagga taataaaaaa
    12001 gcatgtacaa tttcatgcta gcctccaaat ctgggtcaaa aatccaacat ttggaggtca
    12061 tttttttatt tacaagtctt ctaactgctc tttatcaatg atccagactt ctcgattctt
    12121 tctttttaca taaccattct cctctaataa tttaaatttt ctagataaag tttcgggggt
    12181 cgtaccgata aatgcagcaa gttctttcat tttcattggc aggaaaaagt gatccttccc
    12241 tgtcgcttta gctaagtcaa gcagatagac agcgactcgt tcttctactt tttccatcat
    12301 caaaaaagtt gtctgctgct gtacttttgt taatttctca acgttgattg ctaaaagttt
    12361 cagactaagg gcgggataat gcagtaagag ttccgagaaa tctttttgtc tcaatatgca
    12421 aatttccgtt ttttctagtg cttcgccaaa taaagactca tttggaacgc ctagcaactg
    12481 gctctctccc tcatatccgc ccggctctat tactcgtaaa agctgttcct ttccgctagc
    12541 agataattga tacactttca tactgccgga ggccacgatc actaattgag gttctccatt
    12601 cggcgcaaga atctgttctc ctttttccac atagtgatgt ttgacaagtg cgtgtacttt
    12661 ttcttgatct tgctgtacta agtgattgaa caaaggaaca aatgaaacac ataaatgatc
    12721 atgactcata atctctcctc cttctaaaca gctatttcta ataaagaaac ataacttccc
    12781 ttattccaac tgataatttt cttttatcat agcataaacg tcaagcgaac cagataccag
    12841 caaatagagt ggatttcttt ttgtcccttg ctttattttc cttcttttga gaccttaagt
    12901 aattgattcc tatcaatttt ttaaatattt actttttgaa taatcaaaaa caaaccttca
    12961 ctcgcaagcc gagagtaagg tttgtttttc gcattgctta tttcgtgttg tctattgaaa
    13021 tttttccgca cctagctttt gatatagtgg tgctagttct tgtaaggttt cagcggttat
    13081 ttgctcaacg tgttcgattt cttgcggata gaagaatagt ttacccattt gaaaggttcg
    13141 gtctctcatc gcttttttca actcttcttg tttctttgta tcggacaaat agtcagacaa
    13201 agctaagtgg tcgtcaagtt catgttctgg ttgcggccat atgtaataat ccttgatatc
    13261 gactgttttg ctccactctt tcagtatcgc aagcaactgg ttatacgctg caatgctacc
    13321 atatcgttct tcgcttttat aatgttggta catcaaatag atttggtatt gtttttgatt
    13381 caacaaaact gctagacagg cattctcgtc ttgacgagat tcgcttcggt acgcgctcca
    13441 aaaatgactg cgtaaattcc agccattcgt ccagctttcg attttaggtt tgctcatgcc
    13501 gtacatatca ggcaaaagtg cagctgtctg gagctgaatc tctttccatt tttgccagtg
    13561 ttctttatat tctttcttga tttgttcaat cttatctgtt ggatggctct ctttgagctg
    13621 tttgaatgcg tagattggtc gatcaaacaa ctgataatct gattttccaa gttccattgt
    13681 ttcgcctctt tccgttatct tttgtattct acctgaattt tgcgatatgg acaaatagag
    13741 gttgtgaaaa agctgttctg catcaagtca taagaacagc caaacaaaaa actgcgatga
    13801 gcatcaccgc agttttattg aacttaaggt tgattgatca catctaaaag cgttgaaaat
    13861 aaaatggcac tagaagcagc tcctgggtcg atatgtccga ttgcacgctc tcctagatag
    13921 gaagctcgtc cttttttagc taccaagact ttggtgtgtt cttttgcctg gtcaatgatt
    13981 tcttttgtta aattccctgc cttcaatgcc tctgtgactg gcagccaaac atctaacatc
    14041 gttttatcgt cagcttctgc ctttcctcgg ctttgaatac ccgctgttcc ctgctcgatc
    14101 acttcgccta atttttcttg agaatcaata gaatcgacct cttttgtcgc tttcatcatg
    14161 ttcatgaatg cagatccgta taacggacca gaagccccac cgactttcga tatcatcgcc
    14221 atagagaata ctttgaatgt atcgctgatt gtttcaggat gatcggcttg aaaagcttct
    14281 tcataagctg aaacccctct tcccatgtta tttccatggt ctccatcgcc aatcggggta
    14341 tctagatcac tgagatattg tttgttctga ttgattgcct ctgcaaattg cgtgagccat
    14401 tcttggattt cttgaacggt tagtttcata ttatcaccct tttatcttga gattttttac
    14461 catgaaatcg tctgtacatt gctttctaaa gaatctttcc attgtgtctc cgtcaagtcg
    14521 atcagtgtta gagaaagacc ttgcatatct agtgaggtca taaaattacc gacttttcgg
    14581 aaaactacat tgacgcctct ttcttccaac agagctaaaa catcattcat gaacacgaac
    14641 tgttccatca aaggcgtccc gcccattccg ttgactagta cacctgcttc ttttggtaaa
    14701 ttcgagtaat catctaaaat cttagtaaca agttcctttg ccagtacttt agaaggctgc
    14761 atcttttctc gacgatagcc tggctcaccg tggataccaa caccatactc gatctcatct
    14821 tctcctagtt cgaatcctgg tttccctact tctggtacag ttgctgctcg aagcgctaga
    14881 ccgatcgtct tagttgcctg tacgaccttt tctcccagtt ctttcaactc agctagggaa
    14941 gcaccgtttc ttgctgcatc gcccacgatt ttgtgtacaa ggacagtacc tgcaactccg
    15001 cgttttccag cagtatacgt actattttca acggcgatat catcatctac gatcacactt
    15061 tctacttcaa tatcgtccat agcagctaat tcctttgcca tatcgaagtt caatgcatcc
    15121 cctgtataat tcttaacgat cagcaaaact ccttttccac tatcggcttc cttaatagcc
    15181 gtctgaatct gatctggagt tggagaagta aacacatcac caagcaccgc tgcacttaac
    15241 atcccttccc ctacaaaccc ggcatgggat ggttcatgtc cgcttcctcc accagaaacc
    15301 aaaccaactt gttccgcttt ctgattcttg gctacaacac gggaagattc tacacgatga
    15361 acaagttctg gatagctttt tactaatcct tccagcattt cttcgacaat attgcttgga
    15421 tcattcatga tttttttcaa tttattccca tcctttcttt ccggcatttt gtgcttctgt
    15481 taagatttgc tgagtatcat ctgttatacc ggcagtgatg gctgctgcaa aagctccttc
    15541 cactaaaggc gcatccacca gatgataacg ttgctgctgc tcttcttcca acatgtcttt
    15601 tgccagttcc gagttcaata ccgcgctacc gatatccgca aaaacaaaat aactagctgc
    15661 atcgcttgat tcattgactg cttctacgat tttcatcggg tctgatccga tctcgccatt
    15721 tattccgccg agagaaaaaa tttgcacctc ttcggatttt tgcatctgct caatcatttc
    15781 tttaatacca tctgttactt tttgactatg agaaataagc aaaatacttt ttttcacaag
    15841 aatcttcctt tcttttacat agttgctaac gattggtttc gctttcatta atgaacaatt
    15901 caagtatata ataaattttt cgtttgtcaa attaagctag acaaatcatc tttacctacg
    15961 taactcaaaa atacttccaa tggtgtgcga tagtgtaatg attttctagg aatatgattt
    16021 cgttttgatg ccaccgattg gataaaggtt tcggatacct tattaaaatc catagcttta
    16081 tataagccat ctttacgtaa taagccattc gaatgttcat ttagtcctcg ttgagaaggt
    16141 gttcctggat cagcgaaata aatcgaaata tcattggtat tactaatctt tttccaattc
    16201 gagaactctt ttccacaatc aaatgtgatg gatttaaaca agtgtttcgg tacagattga
    16261 aaccattgat tcagtctatt ctcaatatct atagcttgcc taccagcggg tttcaaggtg
    16321 atgatcactt tagataatcg ctcaactaac gtgatcaccg cacttttatg ttttccaccg
    16381 acaatggtgt caccttctag gtgaccaaac tcatgattga actgtatata atcagttgtt
    16441 ctatcctgaa tcgaacggcg aaaagcttgt tttcctcttt tttctttatg tccgtttggt
    16501 tttcttttcc ctttcattgg taaatcgtga gggtcaaagg ttttatcttt aaacatccga
    16561 tatagtgtac gtaccgaaca agcaatagga aactctgctc gaccaataag gacatctgga
    16621 gtccaccctt gagccacctt tttttgaatg tattcttgtt gcccatctgg taacacaatc
    16681 ggacgtcttc cgcagttacg tttgtttttc ttatattgtt catagtagct tattgcgctt
    16741 ccaccagatt taagaaaacg atagacatta taaatggttt gtcgagaacg ttgtaccaga
    16801 tgggctactt ttgaaacaga gtgtgattga ttgaaataag cttctatcat tacgagttca
    16861 gttggtgtaa gatgggtata ggtcatttgt actcactcct tataattctt tcgacggaac
    16921 tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta caatcggcga
    16981 tttctaattt tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa
    17041 gagaggagtg atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta
    17101 gcttataaag ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg
    17161 gaggcggggt tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg
    17221 ggtgcaagag aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat
    17281 gcgatcattg gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg
    17341 gtcacatgta gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac
    17401 taaagagaga tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag
    17461 gtactctacc ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc
    17521 tgcgactaac acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt
    17581 ttttggcaaa gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt
    17641 cgtgcttcca gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg
    17701 gaaactagct gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc
    17761 gctgatcttt ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag
    17821 ctttccagaa gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg
    17881 gatcttcccg tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt
    17941 tttctcaagt tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc
    18001 gattgctgct tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc
    18061 ttttctttta ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt
    18121 tattgatgta tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga
    18181 agtcatttct gtttccgagg caccgttttc agcaccagaa gagcttatgg ttgttgaagg
    18241 ttctgtttga gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact
    18301 ggaaacagca attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct
    18361 tactactttt taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca
    18421 acaggctgtt tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc
    18481 catgtcccgc ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc
    18541 gttatcgcgt tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc
    18601 aattttcttc gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc
    18661 atttcaaagc agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt
    18721 ccttgatata cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat
    18781 attgaccaga caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta
    18841 aaaggatgga gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc
    18901 caacgttttc tgtgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata
    18961 atctcaggag caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg
    19021 tcttctgtcg ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac
    19081 atcgtatttg ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta
    19141 atgatgccca cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga
    19201 ttttggacat ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa
    19261 taattcacat acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa
    19321 atcaatttcc cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt
    19381 ttttcgatcc gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct
    19441 ggtgtttggt ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat
    19501 ttcgtcatca aatactcgat tgtatcttcg atcacaattt cttcattatg ggctggcacc
    19561 atgatcgtga tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt
    19621 ttgtataaaa acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga
    19681 taagaaacga gaaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta
    19741 tgggtgattg agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg
    19801 tcgtatctag atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt
    19861 ttgttccaaa acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt
    19921 catct
//
LOCUS       DACEJZ010000054        19652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEJZ010000054 DACEJZ010000000
VERSION     DACEJZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.056"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            48..365
                     /locus_tag="IW656_001761"
     CDS             48..365
                     /locus_tag="IW656_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0837470.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(523..687)
                     /locus_tag="IW656_001762"
     CDS             complement(523..687)
                     /locus_tag="IW656_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAQ0837471.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            789..1310
                     /locus_tag="IW656_001763"
     CDS             789..1310
                     /locus_tag="IW656_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0837472.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1297..3087
                     /locus_tag="IW656_001764"
     CDS             1297..3087
                     /locus_tag="IW656_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0837473.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKTSLISGLSTYMVA
                     YDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFGKPKRSSINYAPAFSKME
                     PRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTRKQPLIVYITTAGYVLDG
                     PLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPRLWIKANPNICLMNFVGM
                     LDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKRNNKTIDIETLKGKKCVG
                     GFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDNNQERIKAWEKVGDLTII
                     PGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELENAGFETNEIRQGFLSLG
                     GPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWMPSKQSKSRKIDGFAASL
                     NSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            3103..4323
                     /locus_tag="IW656_001765"
     CDS             3103..4323
                     /locus_tag="IW656_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0837474.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            4358..5035
                     /locus_tag="IW656_001766"
     CDS             4358..5035
                     /locus_tag="IW656_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0837475.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            5032..6225
                     /locus_tag="IW656_001767"
     CDS             5032..6225
                     /locus_tag="IW656_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0837476.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            6240..6566
                     /locus_tag="IW656_001768"
     CDS             6240..6566
                     /locus_tag="IW656_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0837477.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            6570..6905
                     /locus_tag="IW656_001769"
     CDS             6570..6905
                     /locus_tag="IW656_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0837478.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            6902..7297
                     /locus_tag="IW656_001770"
     CDS             6902..7297
                     /locus_tag="IW656_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837479.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            7294..7665
                     /locus_tag="IW656_001771"
     CDS             7294..7665
                     /locus_tag="IW656_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837480.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            7681..8241
                     /locus_tag="IW656_001772"
     CDS             7681..8241
                     /locus_tag="IW656_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0837481.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQPDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            8366..8677
                     /locus_tag="IW656_001773"
     CDS             8366..8677
                     /locus_tag="IW656_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837482.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            8701..8892
                     /locus_tag="IW656_001774"
     CDS             8701..8892
                     /locus_tag="IW656_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837483.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            8892..12740
                     /locus_tag="IW656_001775"
     CDS             8892..12740
                     /locus_tag="IW656_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0837484.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETIRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQSATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            12741..13613
                     /locus_tag="IW656_001776"
     CDS             12741..13613
                     /locus_tag="IW656_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837485.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            13610..14659
                     /locus_tag="IW656_001777"
     CDS             13610..14659
                     /locus_tag="IW656_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837486.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            14663..15487
                     /locus_tag="IW656_001778"
     CDS             14663..15487
                     /locus_tag="IW656_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837487.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15505..17565
                     /locus_tag="IW656_001779"
     CDS             15505..17565
                     /locus_tag="IW656_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0837488.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYGTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            17728..18174
                     /locus_tag="IW656_001780"
     CDS             17728..18174
                     /locus_tag="IW656_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837489.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            18176..18313
                     /locus_tag="IW656_001781"
     CDS             18176..18313
                     /locus_tag="IW656_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0837490.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            18348..18590
                     /locus_tag="IW656_001782"
     CDS             18348..18590
                     /locus_tag="IW656_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0837491.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            18606..18803
                     /locus_tag="IW656_001783"
     CDS             18606..18803
                     /locus_tag="IW656_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0837492.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            18814..>19652
                     /locus_tag="IW656_001784"
     CDS             18814..>19652
                     /locus_tag="IW656_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0837493.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGISKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANP
                     TLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYR
                     PDV"
BASE COUNT         6766 a         3400 c         4413 g         5073 t
ORIGIN      
        1 ataagtatcc agatataaaa acaatcggac acaagagaga tatgcaaatg aaagaagcta
       61 gacctagaga cgagatagac aaactataca agaccaaacg atggcgagac ctaaggcaag
      121 tagtaatagc tagggacttc ggcatgtgcc aagagtgcaa gcgtcgaggg cggaacacaa
      181 ggggcacgat catccatcac atagtcgagg cgagggaaga cctgtcactg ttctggtccg
      241 tagataacct tgaatgtatc tgtgtagctt gtcacaacag agagcatcca gagaggtcag
      301 gcgggaagaa gaaaccaaaa cctaaatcac atatcgttaa aatgtattca actcctgaaa
      361 gataagtttg cagtgaaatg aaggcagccc cccctactct aaaagattaa agagtaaggc
      421 ttgagaagaa cggtgctgtc cttccttcgt aaaaagaccg cttttcaagt tttttggaga
      481 aaaaggaaaa agccgaccaa tttaagccgg ctttggacga agctatttct tagtccattt
      541 gtttcctttt tgagaagtag ggggtaatcg gtcgcctgga tcaattttta cttcttgtcc
      601 gccttggaca tttccaccac gaggacccac ttctttgtag gttccttttg gtttgttgtc
      661 ttcgccgggt ttatagagtt ctcccatagg aatccctcct taacaaattt cggcacagca
      721 ctgccgataa cctaattata aggattgtga taacgatttc aatctatctt ttgaaagaag
      781 gtgatattat gccgcaacca gcgaagagtg caaaattaca attattaaac ggaaacccaa
      841 ataagaagaa taccgaagaa ctccgcaagc gagcggccgc agaagacaaa ttaaaaatgg
      901 ctactgacaa aatcaaaccg ccgttatggc tagattcgct aggaaaggat acctttgagt
      961 ttatcgccga tgaattgctg tctgtggatt taatcagtaa tccggacgtc catacaatgg
     1021 ctctatactc caattggtat tcgcaatacg tttctttaga aaaacagctt cgaaaactac
     1081 aacgagagta caagttgaac tatgcgcttg cgaaaaagga ggcagaggcg agaggtgagc
     1141 cgtttaatga acctaatgaa ttaattggta atccgctctc tcggcagatg gatacagcgt
     1201 ctcggaatct ccgttctttt ggcgctgatt taggactatc accagcagcc agagctaagt
     1261 tagctattaa gatggctgat gatggtggtg atgacgatga cgacttctaa tattttggat
     1321 atgtcttaca cagaacgtgt ggactattgg caaagctatc ttgaggagca agcttcttgg
     1381 ggtggctttt taaaatgtcc atatccggaa ttgttaacta cttggtatgc ggaacgatta
     1441 atcgatggaa gcataccagc cagcaaagaa aatattcaag ctgctaaacg gcatatgcgt
     1501 gatttgcagc gccaaggaac agatgatttt ccttggatct ttgacgaaga aaaaggtcac
     1561 cggcctatta gatatatcga aaaaaaatgt aaaccaactg aaggcgactt tggttcgttt
     1621 gttttgcaac cttggcagca tttcataatt ggatccatgt acggatgggt acatcgtgat
     1681 acaggagagc gtcgcttccg cgaggctctt atttttgttg gacgtaaaaa cgggaagaca
     1741 agtcttatct cgggcctttc cacatacatg gtcgcttatg atgatgaaca aggcgccaac
     1801 gtttacgtat tggcaaatgc tcgtgatcaa gcaagcttgt tgtttgataa ggccgcagaa
     1861 atggtcaaac aatcgccggc gctctttaag aaatttggta agcctaaacg atcaagtatt
     1921 aattatgctc ccgccttttc taaaatggaa ccacgcgcct cagatagccg gaaattggat
     1981 gggctaaaca ctcactttgg tatttttgac gagatccacg agtttacgaa ttacaatctg
     2041 atcaacgtta tcaagaaatc aagaggaacc agaaaacagc ctctgatagt ttatatcaca
     2101 actgctggat atgtattaga tggtccgttg atgtcttatt ttgagcaagg tgtggactgt
     2161 ttggaacatt tggaagatga catcgatgaa cggactttct attatctggc aaaacttgac
     2221 agtgcggaag aggctgatga cccaagatta tggatcaaag ccaatccgaa tatttgtcta
     2281 atgaattttg ttggcatgct agatgactat gttaaggata aaaaagatcc aaaagaatat
     2341 gctgactgga ttaccaagca atttaacttg ttttccgata tcgatgagct gtcatttgtc
     2401 gatatgccta ccattaaacg aaacaataaa accatcgata ttgaaacgct caaaggtaag
     2461 aagtgtgtcg gtggttttga cttgtccgaa acggaagact ttaccgcagc cgttttagaa
     2521 tttccgcttg aaacaggcga ggtattcatt ttgcaacaca catggatccc acaagctaga
     2581 tttgatcgag ataacaatca agagcgtatc aaagcgtggg agaaggtggg agatctaacg
     2641 attattcctg gtgattacgt caattatgaa tacgtcttaa attggtttgt agaaaattcg
     2701 aaaatctatg acattgtaaa aatcaattat gacaaggcca aggcgctacg attaaataaa
     2761 gaactagaaa atgcaggatt tgaaaccaac gagattcggc aagggtttct atcattaggt
     2821 gggccaatgc aaaacttcaa ggaaatgcta ttggacggta aggtgatttt caacaattcc
     2881 aagctttacc gatggtatct atccaacgtc aagctggtga tggatcgcaa ctcaaactgg
     2941 atgccgtcta agcagtccaa gagtagaaaa atagatggtt ttgcagcaag tttgaacagc
     3001 cacgccgaag tgttgaatat gttggttaat cctgtcggaa ccgggaaagt aacctattac
     3061 tcgatttccg atttaatgaa tatgtaagaa aggtgtggag gaatgagtat tttagatcgt
     3121 ttgcgttctt ttggccgagc gaagccgaaa gcgagcaaac aagagtattt tttgaatgac
     3181 ccgggattga taccgtattt agtcggaaaa gatgaaatat cagaagggat tttttccgta
     3241 attagccgtg tatcgaacgt ttttgcgtct ctccctctca aaatgataga tgtggagttt
     3301 ggccaaccgg acgactgtcc tgcatacaac ttgttgagcg aaggccctcg atattttaca
     3361 aagtttgatt ttttccggga cgtggaagtt ttgagaaact accaagggaa tgcgtatgtg
     3421 cagattttcc gaaatatcaa tggagaagta gcagatatgg cgttagtaaa acctggtgct
     3481 tgccatccag tgattgatat ggatagcggg gagctttact accaagtaac tgcgactgac
     3541 aaaggcagtt acaagcaagt tatctatgta cattacatgg aaatgcttca ctttaaacaa
     3601 ccgaggtttg gcggcttgga aggtacagac cccacaaaag tattaacgaa taccctcgga
     3661 tatgatcgag aagtccgaaa aatctcttta agtcagctta aaggaagtaa tgaagggcta
     3721 aaagttaagt ttgctagcaa tatggatgaa gaagctaaaa aagctacagt taaaaacatt
     3781 gctgattttt atcgacaaaa cggtggacta cttgtggaag aaaacggtgt agaaatcgaa
     3841 cgtttacaac gagagctggt agacagcaag cttttagata ctgataaagt atctcgctcc
     3901 agaatcgcta tggtctacaa tgtgccggag catttcatcg ggaataacca gtcgagttac
     3961 tcctctcagg aacagctcaa tatggaattt ttgacgtaca atctagtacc gaccgttaat
     4021 caatatgaag cggaactaaa taagaaaaca ctatcgagaa ccgaaaaagc aaaaggttat
     4081 cgatataagt ttaatgtcgc aagtttgcta agagctgaca ctcaggcaag gggacaattc
     4141 tatcaaatca tgcgacgagg cggagcatac tcagcgaatg atgtccgaag atttgaggac
     4201 ttgcaaccga taaataaaac cggtatggat gattaccata tttccggaga cctatatcca
     4261 atcgatatgg atccaacatt aagaaaaaca acctcgtcta aaagcgtagc cgaaaacggt
     4321 taggtttttt tagtttgcac cgaagggagg tggaaaaatg aaaaaagtga cgttaagcgg
     4381 cgatgtcgtg gataacgata ccgcgtggct ttatgactgg tttgggatca attgtatctc
     4441 accagggaaa atttctgccg ctcttacaga agcagcgggg gatgaagtag aacttgatat
     4501 ctcatcgaac ggtggggatg tcctagcggc aagcgaaata tataccgcta tccgtgccta
     4561 tccgggaaag gtatccggaa atgttgtgag tattgcagca agcgctgcaa gtgtaatcgc
     4621 ttgcgcttgc gaaccgctta gaatctcacc tacggcacac atcatgattc ataacgcatg
     4681 ggtgaccact agtggcaacg ctgaggaatt aaaagccaat gcagaaatgt taagcagtgt
     4741 ggatgagtct attgttaatg cttacgagat caaaacagga ctcgatcgga aaaaacttgc
     4801 tgatttaatg gcgaaagata cttggttaaa tgctcaaaca gcagtagcag aagggtttgc
     4861 agatgaaatt atgtttgcag aagcaccagt aacggtactc aatgcctctc aaccggttat
     4921 tccaaaaaac gcagtaacta agttgaaaaa tttaatactc aaagcggaag caccgcaaga
     4981 agaaacactc ttacagaaaa aactaaaagc cttaaatgga gggaaaaacg aatgaattta
     5041 gaacaattaa aaaatgcgtg ggtcgaggcg ggaagtaaag tctctgactt aaatgcacaa
     5101 ctcaatgcag cattggttga cgatgaaaaa acagaagaag atgtagtaag tttgcaagca
     5161 caagtaaaag cagcacgggc taaacgggac ggattgaaag agcaagtggc aaatatggaa
     5221 gccgaacaag tcttaaacgt caaaaaagaa ccattagata aaaaagatga aaacttgaaa
     5281 aacaagttta tcaaagactt taaagcgatg gtcaatggtg atcctgctat tatggctact
     5341 ttgacatcta atacggatga atctggtaat gctatcggat tgactattcc tgtagatgtg
     5401 caaacgacta ttcatacttt ggttcgtcgg tttgactctt tgcaagaata cgtaaacgtt
     5461 gaaaaagtga ccactaccag cggttctcgc gtttatgaaa aatggtctga tattacaccg
     5521 ttgactgctt tggatactga agacggtgaa atcccagcaa atgatgatcc tgcactttac
     5581 ttgatcaaat acttgatcaa acgctatgca ggtatttcca cagtaactaa cagcttgcta
     5641 aaagatactg ccgaaaacat tttggcatgg ttgtctaaat ggatcgcgaa aaaagtagtt
     5701 gttactcgca atacaaaaat cttggcagct attgatggaa tcaaagcggc acaaaagaaa
     5761 gatgttacag atgttgatgg aattaaagat atcgtaaacg tccaacttga tccagctatc
     5821 gaagctacat ctatgtttat tacaaaccaa gatggcttca atgttttaga taaagtgaaa
     5881 cgtgctgatg gatcttactt gttacaaaaa gacgtagctt ctgcaactgg atataccttc
     5941 ttaggtaaac caatcaagaa aattgcttct cgtttcttgc caaacaaagg tacgcaagct
     6001 actcctaaat atccactgta cattggtgat ctgaaagaag ccgttacatt gtatgatcgc
     6061 gaaaacatga gcttgctgac aacgaatatt ggtggtggag cttttgaaac agacaccact
     6121 aaagtacgcg tcatcgatcg cttcgatgtg caactagttg atgatgaagc ggttgttttg
     6181 gctactttta caactattgc gaacgagaca ccggcgaaag tttaaggagc tgattcctta
     6241 tgattcttga tcctaaaatg gatttaggcg aaatcaaaaa cgcactaaag attgataccg
     6301 atgatgacga tgtggaagta agccgtgcgg cacaagctgc aattgcatac attagagggg
     6361 ctatcgggaa tgataagccc tctttttata agcaagaaaa tgaaacggtt gatctgatta
     6421 atttagctat cctgcaatta gcagatcatt attacaacgc tcgatcagca accgtgagtg
     6481 ggaacttgcg agagtacgat ttaggtttta caagcctaat cttgcaactc aaagcaagtt
     6541 atttgctttt tgtggaggag gagtagcgta tgccccttat ccaaacagga aatttaaatc
     6601 aacgcatcaa gtttgtccga gatacgacgg ttaaggatga ggacgggcaa gttgtcccga
     6661 cttctacaac cattcttact tgctgggcaa gtgtgcagac acaacgcctg aacgatatta
     6721 agacgtcgat tggtacggct ttggaaggaa cactgacgtt cattatccgc taccaacaaa
     6781 aatcagagct gaccaatgat atgaaagtgc gttggaatgg aaaaacgttt gaaatcatta
     6841 cgattacgaa aggcgagttt gcgaaggact tcacgacaat cattgcaaaa gaggtttcaa
     6901 aatgagtgta gaagtcgatg caaccgaagt gtacaaagcg cttagggaag taaaagcaaa
     6961 cgttcaacga gtggaaagcc cagcacttag aaaagctggg gagtacgctc aagaagagtt
     7021 acgacaaaac acaccttact gggatggaac gaagtcaaac ggtaaacgtg gttcgtatat
     7081 gcaagaacat gctaagaacc atgtggttac aagctcgata aaaaacggat tggtagaagt
     7141 cggctatgac aaagatgttt cttggcggat gcactttatc gagttcggaa caatcaaaca
     7201 acgtccaaaa ggtttcgtac aaaaaacaca aaagcaaatc gaaaaacaag taacacaaat
     7261 cattgctgac gaagtaaaaa ggaggctagg actttgaaaa cggcagtatc acaagtctat
     7321 tcaattctga atagcaatga aaaaacaaag aacattgatt tttacaccaa tagtgttccg
     7381 gaatcagctc aaacagtacc tagccttcca gttggcagaa ttacagagat atccggcaac
     7441 tatgaagatt ttgcaagcaa caatcctttg accattcaat ttaacgtaca ggtagatgta
     7501 tgggtgtcaa ccatgaaaga ggttgatgcc ttttattttg cccttgatga ggttatgagg
     7561 gggaatggtt ggcaatgcgc atacacggaa caaacagatg acgaggactt ggaaggtgca
     7621 aagcggatta tcaaacgata tgtagcaaat atttcactaa attaaaagga gagaaaatag
     7681 atggcaacag taggattcga gagcgtcatt tttggcgtaa aaacaggtgc aggcggcact
     7741 ctaaaagaat tagtagcaga taagtcgaaa ggcggagcga tcgaagctaa aattactgga
     7801 ttaggcgcaa cttctaacac aacatacgct tcaaacgtac cgttcttcat tgcaagtaaa
     7861 ggggtttcgt cgccaaaagt tacgcttgac gtggcagact taatggataa cggcatttac
     7921 agcgaaatca ttggtgctaa aaccgtggat ggtgcaaatg taattggttc agaaactgaa
     7981 gcgccttacg tgtcggtagt catggttaca gcgaacaaag aaggaaaacg cttgttcatg
     8041 ggattgacaa aaggaaaatt cagtcatcca gatatcgaca tgaaaacagc tgaagacaaa
     8101 ggggtagaat tgcaacccga ttccatcgaa ggggaattca tttctgatga acgtggctat
     8161 gtatacttaa cagccgtaga atcagaagga atgaccttac aaaaattcaa ggacttggta
     8221 aataacaaag agggggagta gttaaccctg catctacacc aacgacagat acagggacac
     8281 caaaagaacc agaaccaaaa attgatacac aaggttagcc atttttggct agccttattt
     8341 tttgtaaaaa caaggaggaa aacaaatgat tgaattgcaa ttgaaacttg acggaaagaa
     8401 aaaaacattc aaacaacaag atatttccgc acgtgcaatg cgtgagtgta tcaaatttta
     8461 cgagaaagcg gaaaaagcag acctaactga tttagaagcg attgattcaa tgattgcaat
     8521 tacagcagat attttccaag atccagcagt tacatttgat gctattttag acggtttgac
     8581 tgcgagcgag ttagtaccgg cattagaaag tgtttttgaa caaataaatg aactgggaaa
     8641 caatgaaaaa aagcagacgg cgagcaaaaa gaaataagtt tttctgaagc taggaaagca
     8701 atggatcaaa tctacaaaga tttaatcgaa gcaggttgga cgatgagaga tgtggacgaa
     8761 gccgactatc attatttgtt acaccttttt ggagaagtgg agagtggcga agaatatgta
     8821 gatggtgctg atttcatcaa acaattttta tcggctgaag acttagtaaa acttgaggaa
     8881 ggaggtaaat aatggcagga aaaggacaac cggcaggaaa tatcaagcta gggattagtt
     8941 tagatagcac tagttttggt aacacgctgg acgaaatcaa tgcgaaagtc aaacaagctg
     9001 agtcgaatat gcgtgccaat ctaaaggctt atgattcagc aggacgttca tacgaagcac
     9061 ttagtcaaaa gacgaaagac ttgtctacgg ttatggaagg gcaaaacgcc aaagtaagag
     9121 aattaacaaa gcgccgtgat gaagcgatta gcaagtatgg cgaggaatcg aaacaagttg
     9181 ctaaccttaa cacacagata aacaatgcta ccgcaaaata taatgcttac agtcgccagt
     9241 tgaacgacac aaaaaaagaa ttggtgtatt ccaaaacagc cgtcaatgat ttatctaatg
     9301 aaatcaaaga aaatgaacga caaatgaacg ccgaagtaaa agcgttgaaa gccgctggtg
     9361 atgaatctgg tgcttttgaa gcaaaacaaa aagggctagc caaacaaacg gaattatccg
     9421 agaaagctat cgaagaacag cgcaaagttg tgaaactgat ggctgatgag tttggcgatt
     9481 cagcaaatga aaccgaagat gcaaaaaggg cattagaaaa gttagaacga caaagccaaa
     9541 tatctagcag gcaattagaa gcactcaaaa gctccagcga tcaatcagga aaagaaatag
     9601 aagattttgg cgacaagtcc acaaggtcag ctaggaaact ggacggacta aaagacaaat
     9661 taagctcgct aaaaagcgca ttttcgtttg gtgcagttgc tggattagcg cataacgcta
     9721 ttagcagtgt agtaagtggc gtgcaaggtt tggttggcga agcagtaaac gcatcggatt
     9781 cattgatgaa gttttccaaa accatggagt ttgctaactt tgggaagtca cagatagaaa
     9841 gctcgaaaaa agaaatgaaa gactacgccg ataagacggt ttatggttta gaagaaattc
     9901 tgaacacaac cgcacaattg gcatctaatg ggattcctaa ctatacagaa ctaaccaagg
     9961 cggcaggtaa cttgaatgcc gttgcaggcg gttctagtga tacattcaaa tccgttgcca
    10021 tgatgctaac gcagacggca ggagctggga aactaacaac tgaaaactgg aatcaattag
    10081 cagatgcgat accgggtgct tcaggattgt tacaagacgc tatgttgaaa aacggagctt
    10141 atacaggaaa cttccgtgat gcaatggcgc aaggtcaaat cacttccgac gagttcaacc
    10201 aagcaattgt acagttaggt atgaatgacg gagcagttaa ggcagccact tctacagaca
    10261 cattgagcgg ttcttgggag cagatgaaat ccactgtaat aaatgggcta caaagtatta
    10321 tagaaaaaat aggcgttgaa aatatcactg gtttcatcaa cagagtaaca aaagggattg
    10381 aaaattctat tcctaaaatt actcaattta taggttggtt gagagatatt ggaacgtgga
    10441 tcgttgaaaa tagagagcca ctaacatgga ttgtcggaat cataggcgga attacattag
    10501 cagtaaaagc attgaacgta gcaagtatgt tgctggcaat tactggcgga acattggcag
    10561 ccccttttgt ggcgattggt gtagcattag gcgcactagc aggtgctttg gtgatagctt
    10621 atacaaaatc tgaaacaatt agaaatatag tcaatgcggc ttttacagct gtgaaaaacg
    10681 tagttatgag cgttatcaat aatttggtgg aatactacaa aatgttgtgg agcgtgttgc
    10741 agtggctttg ggaaaaaata aaagaatggg cttcatggat tggcaataaa ttcattgaaa
    10801 tgaagaacag cgttgtgaat acggttcaga acctatggaa tggcgtgaaa aacttcttca
    10861 gcaatggcgt tggagatacg tggaataagg tagtcggttg ggtaaaaaac attttcaaca
    10921 aagcaactga attgaagaac aacgtttctg atgtaatcgg taacctgtgg aatggtatca
    10981 aagacacatt ccgtagaggt atcgatacgg tattcaattg gttttcagaa ctaccaacga
    11041 agatgaagaa tgccattatt ggcggtaaaa acgccattgt tgatgcgttc aaaagtattt
    11101 tcaacgcagc acttaaagcg ataggtaaac cagttaacgc aatcatccat ggagcttcat
    11161 gggtactaga aaaattgggc gcagaacctc tacaagaatg ggatgtacca caatacgcta
    11221 caggtacacc agcaggtggg cacccaatca atggtccaat gatggttaat gatggacgtg
    11281 gagcagaaac agttatcaca ccagatggta gagccttcat tcctaaagga cgcaatgttg
    11341 ttctaaacgc accaaaagga acgcatgtct tgacagcaga agagaccgcg caacttcaag
    11401 gttctaaagc tcctaagtat cgttacaaaa aaggtactaa cttctttggt aacatgtggg
    11461 atagtgtgaa aaaggttgct ggtaatgtag gtcacacaat taaaaacgta gtaggtgacg
    11521 tgtgggactt tatttcagac ccgggagcgt tagctagaaa agtacttggg gggttagatg
    11581 tattaggtgg attgacaaag tatccattag aagtaggtaa aggcatccta tctaaagcaa
    11641 caagtgcact gactgaaaaa atcactgggt tgttctcatc tggtaactta gatacctccg
    11701 taggaacaaa tggcgtctat aaatatttgg ctgatgttgc taagtctgtg atgaagaaat
    11761 tcccaggatt tgtggcaact agtgggtata gaccaggtga cccttattca catggtaaac
    11821 gtaatgccat tgatattgca ttaccaggcg tcacaggagg ctcacctaga tacacagaag
    11881 cagcaaacta cgcttttgac aaattcgctt ccaaaattgg ttacgtaatc actaatggga
    11941 aagttcgtga ccgttcagga caatcaggtc aaccagcaac tggtgcatgg gagccatggc
    12001 ccgatggaga tcactatgat catgtgcatt taaacggtgt gaaagaccca caaaacactc
    12061 aaatttcagg agatagcgtg ggaggcagtg gggtagaaag atggcgcaat gtagcaatta
    12121 gagcgttgaa aatgaccggt caatacagta ctgcaaactt aaatgcatta ctaaatcaaa
    12181 tgcgtacaga gtcaaatggt aatcctaatg cagttaacaa ttgggatatt aacgccaaaa
    12241 atggaacacc atcaaaaggg ttgctccaag tgattgaccc aacattcaga cagtatgcaa
    12301 tgccaggatt caacagcaat atttttgacc cactatctaa catcttagct tcaatcagat
    12361 acgcactatc aagatatggc tcactaacaa atgcctatcg tggagttggt tacgcaaacg
    12421 gtggaattgt aaaccaacat caaattgcgg aaatcgcaga aggaaacaag ccagaaatta
    12481 ttattccgtt agataaggct aaacgatcaa gagcgatgca attattgtct atcgcaatgg
    12541 acaaactagg agtttctcct aaaacttatg gaaatgcgac aactgtttcc agtgattacg
    12601 aaatcctaga agccattgaa aaacagtcgg caacaacgaa tcagctgtta tcgttattgc
    12661 tggcattttt caaaggaaat agccgaactg atagagattt agctttagat attcagaaga
    12721 tcttggttag gaggatgtag atgcgaactg tattactgag aaatagaata aacgaagaaa
    12781 ttgatttgtc cacagaagac tattttgcta ctgggttgag taatatgggg tttgaagtaa
    12841 aaaaagaaca tgtgggacag tgggggaatt tcagagaaag cagtgagagc gttgaaatat
    12901 ctgaatttca gtcatctgta atcatttctg tgcatgggtt tcgagaaaaa gaactgtaca
    12961 attcacttgt gcagttccta tcggaaggtc cgtttgaact ggaatttgct tttgacggcg
    13021 aaacaatggt aagaagatgc agtctaaaat ctttaagtaa gactgaaatc gatccgaaaa
    13081 catcgttact aacagacact ttagaactat atttcacatc aaactggtat tccgtaaaac
    13141 gcgaaaaatt aattcagcga ccgaatgtag tgaaaacacg tggtaaggtt tttccgtata
    13201 aaagatctta tatctacact caaaacttgt gggagaaaaa aggtgtattc aaattcaata
    13261 ataattctgt atacctaacg aatagcaagg aacggatgtc cccgctaaaa attcgtgtga
    13321 ttgggaagtg ttcaaatccg tattgggaag taatccaaaa ctcacaaatc atcgcaacgg
    13381 atggatactt catagatatg actgaaacac agactctaga agtatcaagc ctttttgaag
    13441 atacaacggc gattttaaaa gatattgcag gggtagaatc ttctgtctac caacaacagg
    13501 attatacaaa aactaatttt gttcaggccc caactggaga attcagcatt gtgtttcatg
    13561 ttgggggggc agacgtagag attgagttat acgaggagcg tgatctgttt tgattttagc
    13621 agtcacgctt tttcatcgtg atttaatgct ttataacgaa catttattct ctaacagttt
    13681 tgaatttggt gtggacgaga tcaacgaaga ggctagtagc tttacgatgg ataagtacgt
    13741 tcctgtaaaa acaggcgatt ttctactagc aaaatatatt cctagtggaa aatttgcata
    13801 ttttggtgta attacgtcgc aggaagacga aaaaatcagt tgtaaaagct tacttagttt
    13861 agctgatagt gagataccga ctgcacgtgt gtcaggagat aactacgaag agcatatccg
    13921 gcggttgatt gaatactatt tgctgaatga tccaacgaaa caactaaaag atattttaga
    13981 cgtcaaagca gaaagtgcga cctctcattc gtatcaagct accgatacga ataaacacaa
    14041 gttaagtgcg tatattctca atggtttcaa aaaatacaac gtaaaatggt atttcaaggg
    14101 aattcaaaac agaaaaattt atacaggcat acgtgctgta aatgaatcaa tttacattaa
    14161 agacaattct tctgaattta gcgattggga tgtgtttgtt caggcgccgg gcgctggaaa
    14221 cgagaacaag ctattaatcg ttgataaagc aatgaaagat atagagaaac cgataatact
    14281 gtcaacatgg tatttggacg aagaaaacaa tttgacacaa gatggatcga aagaaaatat
    14341 cacgaaacca actgtcaatt tggttaatat ctacgatcaa accgcagaag ataaagcatc
    14401 ctacgaagat gtggcaaaat cagagttgaa aggcaatacg tattcgcatg aaatcaaagt
    14461 gaacgttgta agaaatgcaa aaaatttgaa tgtcgaaacg attgaaacag ggatgtttgc
    14521 cacgatttct tataaaggaa agatatacaa gtcggttttg acagcttggc gaatatcaag
    14581 tgataaggag tttgtggaat tgactttcgg aaacataaga agtcgtttta tggattattt
    14641 tgaagataat gggggataaa aaatggttag caatgtggat ggatatcaat ttgaaaacgt
    14701 gaaagtaagc gcagaaaatg atgctagact ttatcacgtt ttatataatc ggaaaaatca
    14761 ggttattgat ggttacgatc agtctatgaa tttatcttca agcggattaa cagtaaaagt
    14821 tgctgcagga gcagcgatta ttcaaggtcg tatggtcgtt gttcgacaag aagaaagtat
    14881 aacagttcca gcaaactcaa gtggttatat agcattaaca gtggatttga cacaagaagt
    14941 tatacctgga tctattcttc cagaatcgga agaatatgaa tggactaata atcaagtcaa
    15001 gctagaattt ataacaaaag ttataaaagg taatttaaac aatggtgata aggtctataa
    15061 tttaccacta tgctcagtta attctactgg atcaactgtt tcaatctcaa agatatcgga
    15121 tagttacgag ctgactctct ctaaaggaga aattttgtgg agggggactg cgttaatgca
    15181 tgatactcaa actgtccaac cttcaaaaaa aatttggcag acagtttcag gttttttgtt
    15241 agtatggctc ccatacgaaa acgggcaagc aattgaggat agatatgtaa ctacgccttt
    15301 ttataaggag cgtgtaactt ttactaatgt tttaggagaa attgtttcag gatttgatga
    15361 ctatcacaaa aagtggttta gtaaacgaat aaactataat tcgaatacta atatatttac
    15421 aggtgcagca agcaatgcaa gtggagataa cgcaaatatg gtgcttaggt atatagtttc
    15481 tttttagtta ggaggtatgg aagagtggca aatttagaaa ttaaattatc tgcaaataag
    15541 agacagcctt atctacgtca ccgtgttgtt ggtagagttg gtgatggggg gctcacaaca
    15601 atcaatgtac aacttcttga ggaagatgaa attacacctt ttgtaattaa tctaaacggt
    15661 actttgaaat ttgtgggcga agtttcaaac ggtaactata ccgagggaga accagaaata
    15721 atcgattcga ctaatgggtt aattagttac acgttcacta agtcaaattt cagcacgagt
    15781 catcaattca aacaagcata ttttgaatat gtagatccta acggcaaaaa agtaactttt
    15841 cagaacttca tcatagacgt gttagaacga gtggatatta attcggagca agcgaaatac
    15901 tatatttctt cattggaaaa attacagagt gaaatgcaaa ccactttcaa tcagttcatt
    15961 agtgataaac aggtccaata cgatcaaatc tactcgaaat ataacgaatt agtaagattg
    16021 ataaatgaat cagataagca agtaaatgat cgtattgatc aaaccaatca gcaaatcggc
    16081 gatctcggca agctgaaaaa gatgtacagt aacagcatcg actttggggg ctatgattat
    16141 tcggggaatc cgaatttgtt aagtaagcta tcatacgact taattgaaaa tcaaaatact
    16201 tcagctggaa cactttctaa gggtgaaaac tcgtttaaat ataataagat atcagctgaa
    16261 gtggaaggtg gagtagagtt atattataaa cgaagaggta tagctaactg gttaccctct
    16321 aataaaacgc ttgtaatgac tgttaagctt agagctggag cggactatag tccagttgac
    16381 ggaaaactta tactgattag atataggtat gttgacagtg gaactggcaa gattgtttta
    16441 gacttaccta ttaacagtaa ttcgataact caggaatgga aagagtttag tattactgga
    16501 actactccaa catttagccc acaagcatac catccttgga tacaatttag ggctcaagat
    16561 ggggtacttg gggaaataga aatgagctat gacatcaaaa tcgaagaagg ctctacagct
    16621 acaccatatc agcctaactt acttgatgac tcttactggc taggtaaaac accgttgggt
    16681 gagaatttga taacgaatgc caagttccca attataacac gaagcaatcc tatttctggt
    16741 tttgatattt cagaagaatt aataataggt gaaacttata cggtatcttt aaaagggact
    16801 aagccagcta ctaaagaatt ccacttatac tacggagaag caaattacca gcagttgcag
    16861 tatcaggcaa ctttacttcc tgtggaagga ttaacagatg tgtggagtgc cacttttaca
    16921 gccagaaata ctagtgaaac gacaaattta cttagagtag cgttatggca aaaacctaat
    16981 tctgcaacat atggtactgt tcaaattgac tggctcaaga ttgaaaaagg cgacacacgc
    17041 acaccaaata tcagtcagtt taaatacttt ggtgaaggct tgaaagacag taacaatccg
    17101 aacgactaca gctgggatgt cacaccagag tatactgaaa aaagcttgaa taatacggtt
    17161 agtctgactg aaccgcaatc catcgaagga ttgaaaaatt tcgaagatgg tattcaatca
    17221 aaaggaaaat ctgtattgac atcagacgac aacaaatatg aagtcgtaac cttaacagtt
    17281 acaaacggga ataccggatc agcaaagctt tatcgtgaag gaaaaaccgt cactatttat
    17341 ttttttgcgt tgaatgggaa aagcagtggt ggaaatgatt caacgatact aacaattcca
    17401 gaaggctatc ggccaccaat tagttttgag caactggttg gctcgataga ccgttctact
    17461 ttgaacagtg ctcagttatc tattggtgca gatggagcca ttaaatggcg aagaaactca
    17521 agttatggat cggattatac ctttgcaatt acttacacga tttagaaagg cgtgaatcga
    17581 tatgaaggca gcatatagac caattgaacc ttacggattc gagcaaatca ttgtgaatga
    17641 tgaagaacat ttaccggaag aatgcacaga agtcgaacca ccgattccaa attggaaacc
    17701 gaaattcaat tactgggagg gaaataaatg aaaaacattt ggaaatatgg ccgtactggc
    17761 ggagagtatg caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag
    17821 ccaccgctcg aaggggttcg ttcagatggc gaaccgctaa cgattgctga tcagatgttt
    17881 gatcctaaat tgaaccaatg gattatttta gcgaacgcgt tagatcacaa cgatttaaac
    17941 aatctcaaag cgatgtacga ggctcttgaa catgaaaacg gcaacctaaa acagctaaat
    18001 gccaaactca tgctaagcga tgtagcgatt aaacaggaaa atactgcatt gaaagaaaaa
    18061 gctgacagtt tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa
    18121 gacatttcag aaattaaaga gcaactaaat ccagcttcaa agggaggtga gtagtatgtt
    18181 tagttttagc gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg
    18241 tctattcgtt ccactatgta tcacagatga agaagcagat agaattatta gcaaagaaga
    18301 gagcgcatct taagtgatgc gtttttttgt tggaaagttg gtggaacatg aaagaagaag
    18361 cgctccaaga cgttgtggag agattagtaa gaattgaaac aaaattagac aactacgaat
    18421 cacttagaga aaaagctgat agtgcaaaag atttggcaga caaagcctac tcagtagcgc
    18481 taaacaacgc agaagatatc aaagaaatga aagccaataa taaatggtcg tggggttaca
    18541 tgatcggttt aggcattacg atcattggct atttcttgac taaattgtaa aggagttaag
    18601 aagaaatgat tttacccgat aagtattatc aagtcattaa atggacggtt ttaacagtat
    18661 taccagctgc atctgtttta gtagccacgt taggaaaagc gtatggatgg aatggaacag
    18721 atatgacagt actcactatc aatgcagtag caacattttt aggcgttatc actggtgtgt
    18781 ctgcatataa cttaaaaaaa taggaggaaa aaaatgaaga agaaaatcat tttatcattg
    18841 agcctaataa tggctctttt tttattgcca attaacgggt tcgcctatac gatcaacaat
    18901 gaatttaatt taggtgtaaa tgaaggtagc tcacaagtag caaataatca gtatatttta
    18961 ctgcatgaaa cggctaatga aacagcgaca ggacgcaatg aagcacagta tatgcaacgt
    19021 tcatggacta gtgcttatac tgcttacatt gtgggagacg gcggaattgt ttatcaagtc
    19081 ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa tagtcctgtg
    19141 cagattgagt tacaacacac acatgataaa gcaacttttg aaaagaacta taaagcatac
    19201 gttgaattgg ctagagattc agcaatgaaa tatggtattc cattaacatt agacactcct
    19261 tataaccaac caggaatcaa atcgcattta tgggtaacgc aaaatatttg gggcgatcat
    19321 acagatcctt acggttatct ttctgaaatg ggcataagta aagaaaaatt agcatatgat
    19381 ttagctcatg gatttaccga tgaaaatcca actacttcag atgataaacc agtcattgat
    19441 ccaactagag caggtgctgc aaatcctacg ctgacagatg gaacaaatta cgcccacatt
    19501 gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag caggatggca tattgctaac
    19561 tataaatacg agtatatttt cattatggat tacaatactg gaaaagaatt agctcgagta
    19621 aaagctgatg ggatttatag accagacgta aa
//
LOCUS       DACEJZ010000055        19452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEJZ010000055 DACEJZ010000000
VERSION     DACEJZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.057"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..3660
                     /locus_tag="IW656_001785"
     CDS             133..3660
                     /locus_tag="IW656_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0837494.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IW656_001786"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IW656_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0837495.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IW656_001787"
     CDS             7448..7789
                     /locus_tag="IW656_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837496.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IW656_001788"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IW656_001788"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0837497.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IW656_001789"
     CDS             9146..11566
                     /locus_tag="IW656_001789"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0837498.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IW656_001790"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IW656_001790"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0837499.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IW656_001791"
     CDS             13000..13737
                     /locus_tag="IW656_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837500.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IW656_001792"
     CDS             13751..14566
                     /locus_tag="IW656_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0837501.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IW656_001793"
     CDS             14579..15226
                     /locus_tag="IW656_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0837502.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IW656_001794"
     CDS             15242..15898
                     /locus_tag="IW656_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0837503.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IW656_001795"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IW656_001795"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0837504.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IW656_001796"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IW656_001796"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0837505.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IW656_001797"
     CDS             18240..18758
                     /locus_tag="IW656_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0837506.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IW656_001798"
     CDS             18865..19365
                     /locus_tag="IW656_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0837507.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4177 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACEJZ010000056        19408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEJZ010000056 DACEJZ010000000
VERSION     DACEJZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.058"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            165..359
                     /locus_tag="IW656_001799"
     CDS             165..359
                     /locus_tag="IW656_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837508.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            628..927
                     /locus_tag="IW656_001800"
     CDS             628..927
                     /locus_tag="IW656_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837509.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1024..1458)
                     /locus_tag="IW656_001801"
     CDS             complement(1024..1458)
                     /locus_tag="IW656_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0837510.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1690..2400)
                     /locus_tag="IW656_001802"
     CDS             complement(1690..2400)
                     /locus_tag="IW656_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0837511.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2390..3250)
                     /locus_tag="IW656_001803"
     CDS             complement(2390..3250)
                     /locus_tag="IW656_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0837512.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3255..3893)
                     /locus_tag="IW656_001804"
     CDS             complement(3255..3893)
                     /locus_tag="IW656_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0837513.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3908..4207)
                     /locus_tag="IW656_001805"
     CDS             complement(3908..4207)
                     /locus_tag="IW656_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0837514.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4250..5734)
                     /locus_tag="IW656_001806"
     CDS             complement(4250..5734)
                     /locus_tag="IW656_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0837515.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5754..6215)
                     /locus_tag="IW656_001807"
     CDS             complement(5754..6215)
                     /locus_tag="IW656_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0837516.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="IW656_001808"
     CDS             complement(6233..7300)
                     /gene="ulaG"
                     /locus_tag="IW656_001808"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0837517.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7518..8288
                     /locus_tag="IW656_001809"
     CDS             7518..8288
                     /locus_tag="IW656_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0837518.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8561..9388)
                     /locus_tag="IW656_001810"
     CDS             complement(8561..9388)
                     /locus_tag="IW656_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837519.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9956..11191
                     /locus_tag="IW656_001811"
     CDS             9956..11191
                     /locus_tag="IW656_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0837520.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12190..12450)
                     /locus_tag="IW656_001812"
     CDS             complement(12190..12450)
                     /locus_tag="IW656_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0837521.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12477..13778)
                     /locus_tag="IW656_001813"
     CDS             complement(12477..13778)
                     /locus_tag="IW656_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0837522.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14070..14528)
                     /locus_tag="IW656_001814"
     CDS             complement(14070..14528)
                     /locus_tag="IW656_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0837523.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14541..15866)
                     /locus_tag="IW656_001815"
     CDS             complement(14541..15866)
                     /locus_tag="IW656_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0837524.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15883..17295)
                     /locus_tag="IW656_001816"
     CDS             complement(15883..17295)
                     /locus_tag="IW656_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837525.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17312..18076)
                     /locus_tag="IW656_001817"
     CDS             complement(17312..18076)
                     /locus_tag="IW656_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0837526.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18180..19055)
                     /locus_tag="IW656_001818"
     CDS             complement(18180..19055)
                     /locus_tag="IW656_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0837527.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19310..19394)
                     /locus_tag="IW656_001819"
     tRNA            complement(19310..19394)
                     /locus_tag="IW656_001819"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19358..19360),aa:Leu,seq:caa)
BASE COUNT         6176 a         3990 c         2986 g         6256 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      541 caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt atttttataa
      601 tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga cagtgaacta
      661 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
      721 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      781 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      841 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      901 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      961 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
     1021 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
     1081 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
     1141 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
     1201 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
     1261 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
     1321 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
     1381 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
     1441 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
     1501 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
     1561 aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt tgtgataaat
     1621 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1681 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1741 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1801 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1861 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1921 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1981 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     2041 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     2101 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     2161 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     2221 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     2281 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     2341 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     2401 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     2461 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     2521 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     2581 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     2641 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2701 tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg atttctaaaa
     2761 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2821 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2881 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2941 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     3001 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     3061 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     3121 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     3181 caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca taaatcccaa
     3241 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     3301 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     3361 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     3421 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     3481 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     3541 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     3601 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3661 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3721 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3781 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3841 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3901 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3961 tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct gcctgagtaa
     4021 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     4081 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     4141 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     4201 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     4261 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca ggatttttcc
     4321 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     4381 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     4441 tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     4501 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     4561 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     4621 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4681 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4741 ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga gttagttcat
     4801 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4861 tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct tgaatcaaat
     4921 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4981 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcaatttttt
     5041 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     5101 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     5161 tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa caacctaaaa
     5221 tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     5281 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     5341 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     5401 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     5461 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     5521 ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     5581 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     5641 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5701 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5761 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5821 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5881 tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc cccttcactt
     5941 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     6001 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     6061 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     6121 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     6181 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     6241 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     6301 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     6361 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     6421 ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     6481 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     6541 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     6601 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6661 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6721 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6781 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6841 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6901 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6961 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     7021 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     7081 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     7141 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     7201 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     7261 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     7321 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa cccttttata
     7381 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     7441 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     7501 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     7561 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     7621 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7681 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7741 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7801 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7861 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7921 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7981 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     8041 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     8101 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     8161 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     8221 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     8281 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     8341 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtatcc
     8401 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     8461 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     8521 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     8581 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     8641 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8701 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8761 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8821 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8881 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8941 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     9001 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     9061 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     9121 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     9181 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     9241 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     9301 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     9361 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct
     9421 cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa
     9481 aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct
     9541 ttttgtgtta tctgaataat tatcgtttac agttataaca catagtgaaa attgtcatgg
     9601 cgagaaagtc taagaatatt tttaaacata actatttctt tcttacgtgt gtataatgac
     9661 gacagcacac ctatcaatta atataattat tttatttttt tatgttctca tgtttatttt
     9721 taatgacaca acttctcgta tttatctggc acaacatata aatcgatcaa aaaattgttg
     9781 cgtgtttttt tgaaaattgg agtttcaact tgattacaaa atttaaattt agaagttgtg
     9841 aaaacagcga gagacagttt aatattcaaa atatacatag gctactcgaa cgctgtgttt
     9901 tttagtatac tagtaagaaa cgaagggata gactcattcc aataaggagg atttcatgaa
     9961 gacaattctt tttgctcctg cagtattcaa tttagctgag acaacacgaa tgattgaggt
    10021 tgccaaagct atgaaagacc gttattactg tgtattcttt ggcttttcta aaacttttgc
    10081 tcctttgatc acagaaagtg gctttgagta tcatttacta gcgccgatcc ttacgaaaaa
    10141 acaagaagaa caaatcataa aatttgacca gttgcgtaca cttaaaaacc cttttaccaa
    10201 agaaatggtt gcctctcgta tcactagtga acagagtctg attgctaagt ttgagcctcg
    10261 actgattgtg acaggttcga atgtgacaat ttttttatcg gcccgcaata aaaagattcc
    10321 tttagtatat gttaaaccct atgctttgag tgaacctttt tttcaatcag agcatgcgct
    10381 gatgccccac gagttacgtt tcttcggccc tttaagcaat cccctttggc actggacaaa
    10441 aaagcgtata gtaaataccc gatggatccc caatgccttc aaacacgctg cacaaaaaga
    10501 aatgcttcct ttaccaaaaa caagtttaca aatgatggat gctgatctaa atctcatcac
    10561 aacgcctaaa ctatttttgg aagaagaaca gctgccggaa aattatcgaa tcgttggtcc
    10621 aatttttgct aagctcagta cttccttgcc tgaagaagtg ctaactttta tacagcgaaa
    10681 gcaacaagaa agaaaacgta ttatctattt cgcaatgggt agttcaggca atccgcgtat
    10741 gttacgcaaa attttgttct tcttacaaaa acaaccggat ctagcggtta ttgcacctat
    10801 tgactatctg cttgatgaca ttcaatctga ctcagaagat cttttaataa cagaatatct
    10861 gtctagtcat cttttgcatg aattcattga tttttctttt atccacggcg gggaagggac
    10921 cgtccaaact gcctgtgaat ccggcaaacc gtttatcggt gtggggatgc aatttgagca
    10981 aagctgtaat attgactatt gtgtaaaata cggaaacgca atcgccctca caaaaccagt
    11041 gactgaaaga aaactgcaga ttgctctgcg cgaagtttct tcacccaaga ttcgtcagca
    11101 ggcacgcacg ctgcaagccg tttttccaat aaatggtcca caacaagcaa ttcaagaaat
    11161 cgaaaccttc ctgagcaacc acccaatata gtttcaagta gaaaaagctt ttttaataga
    11221 gactcatcag taatttcgct attcgagacc cagacagtga aaatacaaaa aaagtaaata
    11281 gtggattgaa aagttagagt tacttaaatt acggtgtcta aaaaacacaa aatcatcctt
    11341 aaaataaaag atctgacgct atccaaatga gcataggtag cgtctgccat ttattcttag
    11401 tcaaattctt tgacaccttt ctctggttgg atcatctcaa cgtttcttca aaagaaagac
    11461 cataataatt aggcaaatac attttcttaa aatgtgtcat atagatctag aagcatcgct
    11521 ttgtagagat ttgtgccgct agtcccctca acaattacta aatcaaatgt aggatttacc
    11581 actttatctt tgataatcaa cctccctctt ctgttgagac aatgatctcg caatccgatg
    11641 catactccta tataaactgc cagcaatccc cacatattga gtagatcatg tcagaatgtt
    11701 ctatgtcgtc agcaacgata cactaaaagt cacgttcacc atgactgatt gctattccca
    11761 gcacaattat ttctatacac gtttcatgat ttgaataaac actcacatca tacatattac
    11821 cattcataca ccatactgca gcccctacta tatggttgta agctcccaaa tctatatttg
    11881 acttgatcac tttggtagta gatctactaa ttctaaatcc attgctgata aaatttccat
    11941 tttttcctcc ttctttctgc caaaacagtt aaaacaataa taatctttat gtaaaaaagg
    12001 gctgtgacaa aagccttgtc acagtcccat attccgaata aacggtggtc aaaagcttgc
    12061 atctgcggta ataagttcaa ccaaacgaaa aataagccaa catttcacaa aaatttaaga
    12121 agcaattttc gtaaatttct tcttatttct tgaagctaac cacttctgtt acaaccactt
    12181 ttagaacatt taaagcttcg atgtgacaaa ccatctatgc tgctcgatca taaaattgat
    12241 agcaaaaatt ccacaaagta catatgtccc ccatgaaaac aaaacattca tatgcggtac
    12301 aatgatcttt ggaagaatca cgatcaaaaa tgatgccagt gcccaaatcc atcgattgat
    12361 ttgaatatga tatgtagcta gcatattcaa tataaggtga ctagccgtta ataaaaagat
    12421 aaaaacaaaa aataaaatca gccaatacat tgtttcccac cttctaaaaa ccaaacttag
    12481 acagtctgga cttcgttttc tccacgactt tgttcatctg acagcatctt tttatcataa
    12541 gcttttacaa atggataata gataataaat gctatcaatg tgttgatcat gatcaagata
    12601 acaacacgcc agtcatttcc tgctgaaaga taaccgccga ttggagctgg taatgtccat
    12661 gttggcaaaa cagtaaatct attgaccatt tctaatttga ctgcaaaata actgataata
    12721 gtcaaaacta aaggaactaa gttgaacggg atgaacatgt aaggattcaa catcatagga
    12781 gctccaaaaa ttatgggctc attgatattg aagataccag gaataataga aaaacgacca
    12841 acctctttca agaattttga tttacagaag aatataaaca aaacagatac cacgatcgtt
    12901 gctcctgcac caccaattgt taccgtccat tgataaaact gttcaggagc gatatagggt
    12961 aattgggtgg cagctgtacc atctgccaat gcctttgcat tctcatccaa catgatcagc
    13021 cacattggac gaacaacagc ccctacaata ctcatgccat ggataccaaa tgaccacaac
    13081 aaatggatca aaaacattgg tagtaaaaca ccaagcaaat tattccctaa aatattggtt
    13141 aaaggctcga ataacgataa taaggcagcg tttacatcaa agcccgcgac gcttcgaatc
    13201 agccaagtaa ttgttaaaat gactgcacct ggaatcaatg cctcaaaact acgggcaaca
    13261 gcaggtggca cttgttctgg cattttgata gtaatatttc gatttttaaa gaaacgatat
    13321 acttccactg caaaaatcat agataagatc cccgcaaaca tcccagatgc tcccaaagaa
    13381 ctcataggta agacccagcc aagaggtgcc tcttcaggag ttaaaacgtt tacaggaact
    13441 tgtaacataa agaacgttcc taaagataat ataccacctg tcacactatc taatccatat
    13501 gatttagcta gatccgaacc cataacaaaa gttgcataaa tagccatcaa tcccatcgtc
    13561 atcctaaagg gtaaaacaat cgttgccgaa taaggggcaa taaattcatt ccatccagga
    13621 aacggtaaat tgataaagac cataaaaaat gtcccaatca aaatcaatga caatgttgaa
    13681 acaaccccat tacgaattgc taataaatga cgctgtgtac caatttttgc cattggtggg
    13741 acaagtttct cttcgaccca tttcatcagc ttttccacta aaaccactcc ttttcaataa
    13801 aatatgaacc attcattgca tgacaacgca aagaatgttc ataactaatt atataattaa
    13861 gaaattttat ttcaatatat tctcttaaat aatcaaattt atttttaaga atcctgaaaa
    13921 taagttttct aaatcatagg agcaacaaaa aaattcgata attaacaaaa atcaaaatga
    13981 ttaaatctag tgattgaaac atatcatttc aaaaaaattt tgtaaattca cccataaata
    14041 tcaccctatt taactttttt catcgtacac taaaattcaa aacaactatc tttaatcttt
    14101 acttcttggt tttgttttaa taaatttcct tcaaaaatgc ctggagaaac tacaatgatc
    14161 accgtatctt tttgtgcttg ctccaacttc ttcaaatcta taatagctaa cagtgtccct
    14221 tcttttacct cctggttact ttcaacccgt atctgaaacg gacttccttc gagttcaact
    14281 gtatctaaac ccatatggat cagtattttt ctgccagatt cactttcgat cgttatggca
    14341 tgtcgggtag gaaatatatt atcgattatt cctttaactg gcgcatggat atatccatca
    14401 tgattcgtta tagcgtatcc aactcctatc atttttgaag aaaagacctc gtcttttact
    14461 gctgttaatg gaataatttg accactagct acagctttta gaatgtttct tgttttaaag
    14521 aaatgcactt tttatcacct ctactttaat gctggccaat attttttgtt tactttgatc
    14581 atttcatcta gtattttttt tgcgactctg gcatttggta cggtttgatt caatgtaaat
    14641 gcttgtaaag ctttttgata agaaccttca aagtaagcat ctactagtaa cttctctact
    14701 gcaacttgtg cctccatcaa tcctttatga aaatcactga ttggttttct tagtgtaatt
    14761 ggctccacac cagttgcacc aacatatgcc ggtatttcaa caactgcatc tgaccgtaga
    14821 ttcggaatcg ccccttgatt aggaacgatt agcataaagc gacgacgatc atcattcaac
    14881 aaggaaatag ctatatcgac gatatattgt ccatgctccc caaaattaaa gttcaatact
    14941 tcttccacaa taccgcggcg tattttatca gccatttctt tggtattttt ctctcgtcca
    15001 tccatgacca tattagcacg tgtatactct ttgtcagtat gctcaacgac catatcagga
    15061 tataagtaat actctaagta attattcggg atattacttg gaaaattttt cgtgatgact
    15121 gacatcattt gaaaggtttt ttgccaagtt ttatctccta tattaaaatc ggcgacttgc
    15181 atttcttttg ttattagttt ttcaataatc tctggcataa tttctcgttg caattcttta
    15241 tcataaatcg atgtatacca gccaaagtgg tttaaaccat aatacgtaac gatccaattc
    15301 ttacgatcat aaccgtaatt cactgcaatc gtttcttcaa ttgaaatagt catatcacag
    15361 gcattaatca tttttacatt cggatattta cgacgaacag cttcagaaac gatcgactct
    15421 ggatttgtat aattcaaaat ccatgctttg ggggcatagt cttgtatatg atcgacaatt
    15481 tccaataaac ctttgattga gcgcatccca taagcaaatc ctccgagtcc acacgtttct
    15541 tgtcctacta atccatgttt aagtggaatc ttttcgtcct tttctcgcat ctccaatcct
    15601 ccaacacgaa tttgtgagaa aacaaaatcg catcctgtaa aagcaagctt tggatcatcc
    15661 gttgcacgaa tgcgaatatt tgaaaaacct tttcttttca gcataaattt aacaatcaag
    15721 tacatatcca tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac
    15781 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
    15841 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
    15901 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
    15961 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
    16021 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
    16081 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
    16141 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
    16201 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa
    16261 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
    16321 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
    16381 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
    16441 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
    16501 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
    16561 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
    16621 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
    16681 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
    16741 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
    16801 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
    16861 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
    16921 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
    16981 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
    17041 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
    17101 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
    17161 tccagctgca ccaattactg ggatgttcag catatcgtca gctgataaac gcatgatcaa
    17221 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
    17281 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
    17341 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
    17401 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
    17461 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
    17521 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
    17581 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
    17641 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
    17701 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
    17761 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
    17821 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
    17881 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
    17941 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
    18001 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
    18061 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
    18121 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
    18181 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
    18241 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
    18301 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
    18361 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
    18421 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
    18481 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
    18541 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
    18601 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
    18661 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
    18721 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
    18781 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
    18841 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
    18901 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
    18961 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
    19021 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
    19081 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
    19141 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
    19201 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
    19261 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
    19321 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
    19381 tccgccacgc cggcaaattt aaaaataa
//
LOCUS       DACEJZ010000057        19379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEJZ010000057 DACEJZ010000000
VERSION     DACEJZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.059"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(375..1328)
                     /locus_tag="IW656_001820"
     CDS             complement(375..1328)
                     /locus_tag="IW656_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0837528.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="IW656_001821"
     CDS             complement(1351..2106)
                     /locus_tag="IW656_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0837529.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="IW656_001822"
     CDS             complement(2103..3062)
                     /locus_tag="IW656_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0837530.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="IW656_001823"
     CDS             complement(3055..4005)
                     /locus_tag="IW656_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0837531.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="IW656_001824"
     CDS             4155..4607
                     /locus_tag="IW656_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0837532.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="IW656_001825"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="IW656_001825"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0837533.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="IW656_001826"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="IW656_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0837534.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="IW656_001827"
     CDS             complement(8159..8968)
                     /locus_tag="IW656_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0837535.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="IW656_001828"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="IW656_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0837536.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /locus_tag="IW656_001829"
     CDS             complement(12578..13264)
                     /locus_tag="IW656_001829"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0837537.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="IW656_001830"
     CDS             complement(13612..15393)
                     /locus_tag="IW656_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0837538.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="IW656_001831"
     CDS             complement(15419..16333)
                     /locus_tag="IW656_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837539.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /locus_tag="IW656_001832"
     CDS             complement(16360..17322)
                     /locus_tag="IW656_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837540.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="IW656_001833"
     CDS             complement(17322..18266)
                     /locus_tag="IW656_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837541.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="IW656_001834"
     CDS             complement(18267..19271)
                     /locus_tag="IW656_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837542.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5338 a         4193 c         3432 g         6416 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 aattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa
    19321 ggacattaca cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatga
//
LOCUS       DACEJZ010000058        19252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEJZ010000058 DACEJZ010000000
VERSION     DACEJZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.060"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            912..2162
                     /locus_tag="IW656_001835"
     CDS             912..2162
                     /locus_tag="IW656_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0837543.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2181..3191
                     /locus_tag="IW656_001836"
     CDS             2181..3191
                     /locus_tag="IW656_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0837544.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3201..3854
                     /locus_tag="IW656_001837"
     CDS             3201..3854
                     /locus_tag="IW656_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0837545.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3873..4871
                     /locus_tag="IW656_001838"
     CDS             3873..4871
                     /locus_tag="IW656_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0837546.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            4947..5708
                     /locus_tag="IW656_001839"
     CDS             4947..5708
                     /locus_tag="IW656_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0837547.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5726..6832
                     /locus_tag="IW656_001840"
     CDS             5726..6832
                     /locus_tag="IW656_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0837548.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6822..7055
                     /locus_tag="IW656_001841"
     CDS             6822..7055
                     /locus_tag="IW656_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837549.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7254..7709
                     /locus_tag="IW656_001842"
     CDS             7254..7709
                     /locus_tag="IW656_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ0837550.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7723..8496
                     /locus_tag="IW656_001843"
     CDS             7723..8496
                     /locus_tag="IW656_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0837551.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8483..9613
                     /locus_tag="IW656_001844"
     CDS             8483..9613
                     /locus_tag="IW656_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0837552.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9644..10879
                     /locus_tag="IW656_001845"
     CDS             9644..10879
                     /locus_tag="IW656_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837553.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10904..12013
                     /locus_tag="IW656_001846"
     CDS             10904..12013
                     /locus_tag="IW656_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837554.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12055..13389
                     /gene="gmhB"
                     /locus_tag="IW656_001847"
     CDS             12055..13389
                     /gene="gmhB"
                     /locus_tag="IW656_001847"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ0837555.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13424..15061
                     /locus_tag="IW656_001848"
     CDS             13424..15061
                     /locus_tag="IW656_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0837556.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15163..15558
                     /locus_tag="IW656_001849"
     CDS             15163..15558
                     /locus_tag="IW656_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0837557.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15576..16307
                     /locus_tag="IW656_001850"
     CDS             15576..16307
                     /locus_tag="IW656_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837558.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16331..17782
                     /locus_tag="IW656_001851"
     CDS             16331..17782
                     /locus_tag="IW656_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0837559.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17808..18419
                     /locus_tag="IW656_001852"
     CDS             17808..18419
                     /locus_tag="IW656_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0837560.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18601..19116
                     /locus_tag="IW656_001853"
     CDS             18601..19116
                     /locus_tag="IW656_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ0837561.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6204 a         2687 c         4208 g         6153 t
ORIGIN      
        1 atatatatgt atatggtgac tgaagacaaa aaaacagaac taatgaaact gaataaaaat
       61 atagagtgaa ctagtttcta agagggattt taaactagat ttcattggta ataaaatttg
      121 gagagatgga aaaacagcac ttaaggaagc agattttgtt gtcatttttg ttcctacaaa
      181 ctatggtagt gaatgtgtta ttgaattagt gatgtctgtt atttcagagg cattggggat
      241 aattaaatcc actatacctg taggatatac aaaaagtgga agaaaaaaat attatcaata
      301 gattttattt agttcagaat ttttacgtga aagtagatta ttaatgataa tctttatcca
      361 attagaatta ttatcgtagg atgtgatgat gaaagccgtg aaattgttca agtatttacc
      421 aatgtctccc tgccagtaaa tccgcttaga atttgagcct tgaaaatgtg caatgataaa
      481 aaacaaaaac tcgctcaaaa tagacagttt ggggtgggaa tagaccaata atgtaccaga
      541 caaacttcca tttggggtga aaataggtca ataaagcgat gttaaacata cctttggagt
      601 aagaatagag ggtaataagt gaatttataa actttttggt gactggttga gtggaaaatg
      661 taggtacatt tcaatgcgaa aatgtattat aaccccaaac gaggtcaata aaaatgactc
      721 aaacttgcaa tataacccta aacgggtagg ggatttatga aaaattgaat ttgccggcgt
      781 aggagctggc tgttttaagt atgaatgaac acagctttcg ggctgtttta tataaatagc
      841 aggttactgt tcacaaacag cattttgtta agaaagtagt gaccacagca gtggtggaaa
      901 gaggaaatat tatgaagaaa cagtataaca ttgcagtcgc cggcaccggt tatgttggtt
      961 tgagtattgc cactctgttg gcacagcacc ataaggttac agcggtggat atcattccag
     1021 agaaggttga actgattaat aaccgtaaat ctccaatcca ggatgattat attgaaaagt
     1081 acctggctga gaaagaactg aatctgaccg caacattgga tgcaaaggca gcataccagt
     1141 ctgcggattt tgttgttatt gcggctccta ctaactacaa tagccgcact caacattttg
     1201 acacatccgc tgttgaggct gttattaggc tggtcatgga agtaaatcct gatgccatta
     1261 tggttattaa gtccaccatt cctgttggat acaccgcttc tgttcgtgta aagactggta
     1321 gcaagaatat tattttcagc cctgagttcc tgcgtgaaag taaggcactg tatgacaatc
     1381 tgtatccttc gcgtattatt gtgggtaccg atatggatga tgagcgtttg gttgaggatg
     1441 cacacacttt tgctacactg ctacaggaag gcgcaatcaa agagaatgtg gacacattgt
     1501 tcatgggctt tactgaagca gaagctgtta agctgtttgc caatacatat ctggctcttc
     1561 gtgtcagcta cttcaatgag ctggacactt atgcagagat gaaaggcttg aacactcagc
     1621 agatcattaa tggagtttgc cttgatcctc gtattggtac tcattacaac aacccttcct
     1681 ttggttacgg tggctattgc ctgccgaagg ataccaagca gcttctggcc aactacgcag
     1741 atgttcctga aaatctgatc gaggcaatcg ttgaatctaa ccgtactcgt aaggacttta
     1801 ttgcggatcg tgtacttcag atggctggtt attacaatta cgcagaagca aatcagtatg
     1861 atgctggcaa agaaaagcct gttgtgattg gtgtttaccg tttgaccatg aaatccaatt
     1921 ctgataactt ccgccagagt tctatccagg gcgttatgaa gcgtgtaaaa gctaaaggtg
     1981 caactgttgt gatttatgag ccgaccttga aagatggaga gactttcttt ggcagtaagg
     2041 ttgtgaatga tttggaacag tttaaggcaa tgtgtgattc tgttatagca aatcgctacg
     2101 atgcttgcct tgatgacatt aaggaaaaag tatatacccg tgatatcttc cagagagact
     2161 aagattagga gagagtattt atgattatta caaaaacacc gtttcgtatg tctttttttg
     2221 gcggtggaac ggatatggag gattatttca aagagaatgg cggtgcagtt ctttctacta
     2281 cttttgataa gtactgttat gtgaatgtga gacatctgcc tagatttttt gattacagcg
     2341 ctgagttatc ttattctcgt actgagagag taactagcat tgaagatatt gagcatcctg
     2401 cgattcgtaa tgcgatgaaa atgctcgatg tgcatgaaat tcggcttaca tacgaagctg
     2461 atttacctgc tcgctctggt ctgggtacat ccagttcttt tgcagttggt atgttgaatg
     2521 ctttttatgc actggagggt aagtatgctg acaagaagaa acttgctgat gaagccattt
     2581 atcttgaaag agttctgtgt aatgaagccg gtggatggca ggatcagatt gccgcatctt
     2641 atggtgggtt caatcgaatt aatttcaatg tggatggtta tgaggtgctg cctgtcatta
     2701 tttcgcctga acgtaaaaag cagcttaaca acaatctgat gatgttcttt accggattca
     2761 ctagattttc ttctgatgtt caaaaggcta acaacgtcag tggcacagag gataagagag
     2821 taagactgaa aaagatgtat gagcttgtag atgaagctga ggcaattctt actgataaga
     2881 acagaaattt agatgatttc ggtagacagc ttgatgttac ttggagattg aaaaaaggta
     2941 ctggtggagc aattagtaca ggaagcattg atgaactata tgaaaagggt atggctgcag
     3001 gtgctttagg cggaaagttg ctcggtgcag gtggtggagg tttcttggtt ttctatgtac
     3061 aacctgagaa acaggatgca gttcgttggg ctatgcgtga tttgatgtat attccgtttg
     3121 aatttgaaga tggtggaact agagttattc actacacgcc ggaaacttat attgaaaaac
     3181 agaatggata aggagtaaca atgttagcgg aaaaacttaa taaacatatt gacttattga
     3241 ttaaacgata tcctacactt gagacgatta aacaggacat tatcgatgga tacctaatta
     3301 tggaggaatg ctatttgaat ggtggtaaac ttcttgtggc aggaaacgga ggatcagccg
     3361 ctgactcgga acatattgct ggcgagttga tgaagcggtt taagacgcca cgtcctgtaa
     3421 gtaaggaatt tgccgataag ttagttgcaa ttgaccctgt aagaggtccg gaattagcca
     3481 aaaatctcga gtgtggttta acggcaattc ctttggtcgc acacgaagca ttgtctactg
     3541 cttatattaa tgatgtcgat ggtttgggtg tcttcgcaca gcagctgttt ggctttggta
     3601 gatctggtga cgtattcctt ggtatttcga cgtccggaaa tagcaagaat attatgagcg
     3661 caactgttgt tgctagggct ctcggtataa aagttattgg cttgacgggc gcaaagggtg
     3721 gggaacttgc cacggtagca gatgtttgtg ttaaggctcc tgaagttgaa acatacatga
     3781 ttcaagagta tcacttgccg atatatcatt gttggtgttt gatgctggaa gataaattct
     3841 ttggaaacga ataaaatttg gaggaatgtt aaatgaaaaa gacaattatg gttaccggtg
     3901 gttgcggtat gattggctcc aatcttgtta agagactggt gaaggaaggc tgctgggatg
     3961 tttatgtagc tgataatctg tggagaggaa agttggagta cctgaacgac gaagatgggc
     4021 atcctgtcat cgacctggat actcatttct tcaatgctga tctgactgat tatgagcagg
     4081 ctaaaaaggt tattggtacg actgagtatg tagttcatct ggctgatgtt gtggctggca
     4141 ttgactatgt gtttaagaac cagggcgaat tgttccgaat caataatctg atcaattcta
     4201 atgtttttga ttgttgccgt aaggctggca aggagaaaat caagggtgtt atctatgttg
     4261 gtaccgtatg ctccttccct ctgaccagac agaatacttt gaatcctgag cctctccggg
     4321 aagaggagtt gttccctgct cttcctgagt ctgcttatgg ctggagcaaa ctgatgggcc
     4381 agttggagat ggggtatttg gaaaaagaaa ccggaattcc gtgctgtatc ctgatgcttc
     4441 ataatgttta tggcacacct actgattttg gcgaacgtag tcaggttatt cctgctctga
     4501 ttcgtaaagc aatcaataat cctgaagagc cttttaatgt gtggggtagc ggagagcagg
     4561 gacgtgcttt cattcatgtc aatgatatcg taaatgcatt ggtgctcgcc ctggataagg
     4621 gttggggtca tggtcatatt cagattggcc cttctcactg tacttccatc aaggaaattg
     4681 catataagat cattgaaatt tctggcaaag acatcaagcc tttctttgat acttctaagc
     4741 ctgaaggtga taaggctcgt tgcgcagatt attctaaggc gaaagagatt ttggattgga
     4801 agcctactgt ttctttggaa gagggtcttc gtgaatccta caattggatt gaaaagcaga
     4861 ttaagggtta ataagtatat aagctgttct tcctccgtgt aattagagga tgcggaggaa
     4921 gaacatatga gtaaggatgt actgagatgg aaaagaatat gtataaaaga aaacgtgatt
     4981 tcactgtgtg tggaatagta ggtgtttgtg ctactacaaa gaacgagttt gtaagggaag
     5041 ttttttccat gccaacatct ccggtaagtg cgactgttga tctcgtggga gtgccggcca
     5101 ttatttcggc aaaggaaaat cccaaaattg ctgagatgta tagccaggct acaatggctg
     5161 caatagatgg tatgcctatt gttaaaattg gacggcgcaa aggatttacc tgtgaacgat
     5221 gtgctgcacc agatattatg ggaatggttt ttgaagaagg cgttagacag ggaaaaacac
     5281 attatttcta tggtggaaaa gataacgaag tgttaaataa gcttcgtgca aacctagaaa
     5341 gagattaccc gggaatcaaa attgttggaa tgtattcccc accatttcgt ccgttaaatg
     5401 aagaagagga tagcaagatt tgcgaggaaa tcaacaactt aaatccagat tttctgtggg
     5461 ttggtattgg tgcgcctaag caagaaatgt ggatgtggga acaccagaag aagatccata
     5521 acacagtaat gttcggtgta ggcgccggct ttaacttttt tgcgggcact ttggaaaaag
     5581 ctcctgaatg gatggaaaaa gctagtctag agtggttatt tagactggta aaggaacctc
     5641 gtagactatg gaggcggtat atcttgggag gtataaagta tatctactat actattgaat
     5701 ctaagtaggg gggcaataaa aatagatgaa aatacttata gtagttacaa tgacgaataa
     5761 ctttggtaag aaggggtcct ataattctca ggaaatagga ttagctcgag ggttagctga
     5821 attaggccac gaagttattg tatataaaag tgtaaaaagt gatgaggaag ttggaacaga
     5881 gcaattaagc aatacggcga tgattcacta tatcaagtgt cgttatgtag gatcaaatgg
     5941 attcttatca acaaagaatt ttgataagaa tgcggatgtt ctaatacaat ttgcggacct
     6001 tcaactatca gtacctcatg tatactcatg gactgtaaaa aatggaataa aatatatacc
     6061 ttataatggg ataacgcgaa gtcaaagtgg caataaatat ataaagacaa ttattgaaat
     6121 attggcgcat cgaaattata aaatttataa gaaaaatggg tgtgttgcca aaaatgatcg
     6181 agtttctgaa aaattagtac aattaggagt aaataaagtg aaacttgctc ctgtttgtgt
     6241 ggattttgaa cagttacata agaattacga agcaatagat tttactactc tcagagaaaa
     6301 atggaatatt cctcgcgatg ttcgtgttat tttatatgta ggacggtttg aaaaggataa
     6361 aagaccgtta gatatgatta gtttatttaa taaacttgat gagcataatt atttgctgat
     6421 ggtaggaact ggtgctttac tcgagcaaac gaagcagcag ataaatttac ttggactgca
     6481 gaatagagta aaaataattg agcgtattcc taacaaagat atttgggagt tatattgctt
     6541 atcggatgta tttgtaaatc taaactatac tgaaatttgg ggtatggttt tgctagaggc
     6601 aatgtactat cgcctcggaa ttattgctgt taatgcacct ggcccacaga atattatttt
     6661 ggataataga tatggaatgt tggctaaatc tgacgatgaa attttgacat tcttaaaaga
     6721 cggacgattt accaacgata tcatagatgc agcatatcag cgtgtaacta cagaatttag
     6781 atggtcaaag tcgggagaaa ttatgcttag catgttggaa ggtgataaat gatatggaag
     6841 taacgaaatt aatggcattg aggaatcgat atgctttaaa catagttgat aattgcacga
     6901 gaaaaatagc caaaattctt ggttgttgta ttggtaaagg tgcacagata gggaactctg
     6961 ttgaatttgt tcataattct gtaggcactg tgattcattc tgacacaatc cttgaagatg
     7021 gtgttaaagt ttatcagaat gtgacgtgtg gttgagcaga tagtaatgaa cgtatcatca
     7081 ttcgagtaaa aaagaacgct gttttatgtg tgggtgctaa aattctttgt aaatctaaca
     7141 cagaattatg cgttggggaa ggtgctgtag ttgctgcaaa tacagtggta ttgaggtcta
     7201 ttccacccta cgaagtgtgg ggggtacctg ctaaaaagat ggggagataa aacatgaatg
     7261 catataaatt atacaaagta gaacgatggt tttacttaca caaaatggag ttttttgcca
     7321 agattgtaaa ggcactaatt tatgtacttc ataatagctc aatttcatat caaacagaaa
     7381 ttggagataa ttgtaagttc ttatacggtg gcataggttg tgtaattgga aaagatacaa
     7441 aaataggtaa tcacgttata atcggaacga atgtactgac tggtggtaga tccaataagc
     7501 ctggtatgcc tacaattgga aacaatgtat atatcgctac tggcgcaaaa attttaggta
     7561 atatcacaat aggggacaat gtaatagtag gggctaatgc tgtggtcatt aatgatgttg
     7621 aacctaattg ttcggtagga ggcgttccgg ctagagtatt gaaaaaagat attgatgtaa
     7681 atgattattg tagtttgaat cgttactaaa aggagagatt gtatgttgta ttttgttatt
     7741 cctttaatga gtgcgaaggc agcaaacaat tgggagatgg ttagtacctt atttaaccga
     7801 acattatggt cgtgttataa tcagactgat cctgatttca aaatcattgt tgcttgccat
     7861 gaaattccta aactcacgca tgaatatgat gacagagttg aatttattca agttagcgaa
     7921 aaggatgccc ctattcccac aaatcaagac gaaaaaatga ttgataaggg ttataaaaca
     7981 catgctctcg caatgcgttt gcgagagttg ggcggcggtt tcgctatgat ggttgacgca
     8041 gatgatcttg taagttctca tttggcggaa tatgtgaatt cccatcccca ggaaaatgga
     8101 ttttatgtca aaacgggata cgtgtacttt gttggagacg attatatgaa ggttctacca
     8161 aagttctcat caggaagtgc gtgtatagta aaatatgcag tggaagacct gcctgaggca
     8221 tatccggaag ttatgacagc aaattgtgat gataatccat ggatcattag aaaacgacat
     8281 gggggcgttg ttccagcatg tgaagagcaa gggcgtccat taaaacctct tccattcaaa
     8341 gcagcagtat atgtattagg aacaggggac aatcactcgc tttatggtaa aacgacaaaa
     8401 tatcagacga agatgcgaga gatactggaa atgtttatag caaaacgtaa aattaaagga
     8461 aaactgaaag cagaattttc tgttgattgg ttataaggtg agtattatga aggtattgat
     8521 gtgtgaaagc caaatgccag agaattggta ttcgtttgat ctttctaaag aacttaatca
     8581 atatgttgat ttaacattgc tgtgtaagcg tgatgttcct cctttagatc ctaaaattaa
     8641 gagcaaacat attttgtatc ataaaagtac aaataaagtg ttttcggcac ttgtttttct
     8701 gaaaagcttg ttcttaattt ggaaggaaat aagatttggt gattatgata ttgttcattt
     8761 gcaattgatg aaaagtctca aatatgaagg cggattgtac gttttatgta agagacacta
     8821 taaaaaatta attatgacag ttcataatgt gttaccacat gaggttcgtg aaaaggatcg
     8881 ccaagagcac tttaatatat attcaaattg tgatggtttc attgttcaca atcgagtaac
     8941 agaacagcat ttatatgctg aattcccaga attaaatgca aagtcatatg tttgtccaca
     9001 tgctatctat ggagatgtta aacatatatc tagagcgtat gataaagata agtttgaatt
     9061 tatcttgttt ggacaaattc gtccatataa gggaattgat gtactgctta atgcaatcac
     9121 agaacttcca atggatgtaa gaaagaaagt tcattttacc atagcaggtc aacaacatcc
     9181 taaacaggat agtactgact ataaaaaaat gattgaagat ttaaaggttt cagattgtgt
     9241 tctatataag ccagagagaa tttctgatga agaaatggac agtatgttca gaaatgcaga
     9301 tgccgcaata tttccatata aagaaatcta tggaagtgga gcgcttctta tggcatacac
     9361 ttattgctta ccaactattg ttagtaatgt acctgctttt atggaagaaa ccgaggaagg
     9421 aaagataggg ttacttttcg aaaatttgaa ttctaagagc ctagcagaca caattgtaaa
     9481 gtatgtgaac cttagcccag ctgagaaaag caaaatagag gatgatatat atgagcttgt
     9541 aaaccgcaag tatacctggg cggcatctgc aaaactcaca tacgaggcat acaaacaaat
     9601 tttgaatgag tagtaaaaca attagaaagg aataataatt aacatgcaga ttagtaaatg
     9661 gaaaatcata cagtttctaa tggtctttct aatgattatc tcatcaagca tattgaagcc
     9721 tttgggcggc tggttgggag aaacgatgaa cgttagactt gtgacagtca ttctagcaat
     9781 agtatgtttg ttaatagcct cattaccagc atttagaaaa aatagatatt taaatttctg
     9841 cactttactg atttgtgttg ttataatagc agcttacatc tactcagata agacaatatg
     9901 ggttaaatct agggaaagta tgagtttata ctatgtatta ttggcgtatc cattgttctg
     9961 tactttaatt aaccaagcgt ggaatttgga ttcaatgcta aatacgatat gtggtgtaac
    10021 atttctttca tacattctta gaacatctat ttcacttatt cagaaatttt caggagtact
    10081 tctctatgaa aacatatatc gtgagtgtgc cccggaaaat tggtatcgtg gaggcttttt
    10141 aagaattaat ccaccatgtt ttacaattat aataataccg attgctttgt atatgattga
    10201 gcgacaagtt gtagctagaa gaaaagtaaa atattattta ctgattgcta gcgcactggc
    10261 atattctgct gttattcatg gttctagatc tgtccttgtc tatcaaatta ttgtacttgg
    10321 ttgctatatc ctttttaaga aaggttcttc gaaaagaaaa attgcaatgt gggttttagc
    10381 tgggttgctt gtagtcatat ttattaattc gaccatgttc tcaacatttg ttgaaacttt
    10441 cacaaacagt acaggtgaat atgcaggatc tgctgaatct cgatttgctt cagtttggtt
    10501 ttttgttcct aaatttttgc aatcgccttt gttcggtact ggtattctta ctggtgagga
    10561 atttacgttt atattaccag gaggagttag aggacaccta tctgatattg gatttttcta
    10621 tacaattact caatatggtg taggtatact tatatttgat attctgttta ttataaaagg
    10681 gttcaaaaca gcattacttg catcaaaagt gggtatgact ggctatcaat atttagcatt
    10741 tggaattaca ttaagtgttt tgctaacagg gattaatata gactggtttt atccgatatt
    10801 tactccagct attcctgtat gcttagcggt tatagaatac atctatcaag aatgtagaag
    10861 tgtggctaac atagaatagg aaatattaat aggagtgcat ctcatgaata agaaagagat
    10921 tttatataac atattgaaga agtacgaaaa tttaccatat gtgtttagga acatgtttgg
    10981 tcataatata agtaaactcg tatcttggaa attgactgct gattgtgcaa cagtagaaga
    11041 aaattctgaa gtttatgcag cgtttcaatt gcgctcaaag gacatgtctg atgttccagt
    11101 gatgggttta gttaattgtt gcaaagatgt agcaattgta atgcaggggc cactatgttt
    11161 aaaggatgat ttctcttata atactctggc ctactataag aaatgttatc caaatgcttt
    11221 aattattctt tcgacatgga aggacgaaga tattaaggca ttgaaaagga tagaggatct
    11281 tggagttata gttatttgta gtgagaaacc tgaaaagcct ggacacttaa atattaatta
    11341 tcaagttgga aacactttgc ctggaatagt cagagcaaag cagcttggtg caaagtatgt
    11401 ttgtaaaact agaacagatc aaagaatata ccatccaaat gcaatggcgt ttttctgcag
    11461 tttacttgaa cagtatcctg ttaataacga agatgattgg aaattaatac aaaatcagcg
    11521 attactgtta ctatcaatgc cgtatggaga catgtttttc ccgtattgtt tgtcagactt
    11581 cctgtatttt ggtgatgttg atgatatgat aaacctcttc tcgattccta gtgatgtgag
    11641 agaaaaggga gctgtgtcta gaggagtctc aagaagggag atatctgaaa ataatttagc
    11701 tccagaagtt cagcttcttc gaagctatat ttcacgaatg gggggcaatg aggagtgctc
    11761 tattagggca tattgggagt ttgtaaaaaa ccatgttatt actattaaca aaaatcaaat
    11821 tgatttgtac tggcataaat atgtaggtcg ttattcaaat aatactattt atggtacgta
    11881 ttatattgat gatagccaag atgcacttca ctgctataat tttgatttta taaattggtt
    11941 gaatttgtac actggaaaat acgaatacaa agcaaaatat gaaaaatata tggactttgt
    12001 ttgggaagag taattaactt ttatagtaaa aagtacttta gggaagggca tattatgaaa
    12061 acagtaatta tggcgggcgg acgaggaagc cgaatttcgg aattgtttcc ggatattcct
    12121 aagccaatga ttcctataga tggcattcca gttttagaac gagaattgtg ttcgcttaga
    12181 gatcaaggct ttaatgatat tattttgact gtttcctatt tgcatgaaaa aatagaggag
    12241 catttcggag atggttcaaa attaggaatt aaaatcaaat attttattga aaaaactcca
    12301 cttggcaatg caggtgcatt gtattttttg ttggatgaac tgggtgatga accgttttta
    12361 cttttgaatg ctgatgcagt atttgatgtt gatttcaggc agatgattag atttcatgtt
    12421 gagaataacg cagatgttac tttgtttacg catcctaact cacatccgta tgatagtgga
    12481 ttaattatat ccgatgacaa taagaaagtg cttcaatggt tagcgaagga agatcaaaga
    12541 cctgaatatt ataaaaaccg agtaaatgca ggtcttcatg ttataaatcc gaaggtgttg
    12601 agagaaaatc cattaatgga aattgtaaat aaatcaatag ttgaaggtgg ggggctacca
    12661 tacaaaattg atttagatag aatgattctg aaaccattgg ctaacacagg gcgtatgtat
    12721 tgctacgatt ctccagagta tgttaaagat atgggaacac cggaccgata tagagctgta
    12781 attaaggact ttcaaaaggg aattattgtt ggtaaaaatc tatccaatcc acaaaaagca
    12841 atctttttag atagagatgg aacgattaat aaatacgttg gttttttgag aaatgcagaa
    12901 gaatttgagt taattgatgg agtatcggag gcaattaaga ttattaattc tctaggtttt
    12961 ctgtgtattg ttataacaaa tcagcctgta attgcaagag gtgaagttac tgttgatgag
    13021 cttaatagta ttcacaataa gcttgaaaca atgctagggc atgctggtgg atttttaaac
    13081 ggtatttatt actgtccgca ccatccccat aaaggatatg caggagaggt tatagaatta
    13141 aaaattgatt gcaattgtcg caagccaaaa cctggactgt tattagaagc atcaaaggat
    13201 tttaacataa acctaagtca atcatttatg gttggagata gtgaaagtga tgtaattgct
    13261 ggaaaaaatg ctggttgtaa gacagtactg attacagaag acatcacctt gcctgctaat
    13321 tatggccaag actatagtgt ctgttcattg ttagaatttg ttaaaaatca tttatgcact
    13381 atgcaataat tggtttagtg aaacttgtaa aaggagtaat tagatgaagg tattaattcc
    13441 agcagcaaaa gttgttgaaa aagaattgca gagtatagga atgttaccag caattatcta
    13501 tccaattaat aaaaaaattg tctttgatta tttgtataat cagtataatg caatctgcga
    13561 ttcattggat attatctgtt atgaaaaagc ggataaggtt catagaagac tgaaaaacta
    13621 tcatgattcc agaattcata tatgtgacct tcctgaatta ggggatctag ggcaaaccgt
    13681 gtattttggg ttatcagatg aagatgagcc tgttctaatt aacttcgcag atacaattgt
    13741 tatggagagt atgtctgata tagaaatgga tacattctat tattctgaag attttgtgtc
    13801 gagtacttgg acatacttta aaattaatga aggaataatt actgatatcc acgataaaga
    13861 ggaagcttac gtatccgata aacgtgaaaa attatttatt ggcgtattta tgttttcttc
    13921 gtcaaaactt ctgaagagtt gcttaagaga ggcagttgag agtcggaaac aggggggaat
    13981 aaactcattt tatcgggctt tacagctata cagcgaacgt tgtccgctaa aggccgtaag
    14041 aacagaaaag tggtttgata ttggacacat cgataaatat tacaatacaa agttggaagt
    14101 taaagcacga gagtttaatc atatctctat agataaagat cgagggatat tgaagaaaac
    14161 tagcgatgac aaggataaat ttattggaga aataaagtgg tatttaaaac ttcctgctga
    14221 tattgaatat gtaagaccta gaatttttga ttattctaca tcttacgtag ctccctatgt
    14281 tgatatggaa tattatgcat atcatacggt tcatgagtta tacttatatg gtgatttaaa
    14341 atataaccag tggcatgatc tgtttagccg tataaagttt atctgtagcg attttaagcg
    14401 atacacctta aaagatgaaa agattagaga gtcattggaa gatatgtatt tgcgaaaaac
    14461 catgcagaga tttcaaaaat tgtctgaaga taaaagattt tcagcttttt ttgagaaaca
    14521 aattcaaatt aatggtcgta agtttatttc tttaaccggt gtaattgaac ttctaaagaa
    14581 tcttatccca agtatgctct atgatgtaaa tgaattttgt attatacatg gagatttgtg
    14641 ttttgctaat atgatggtag attccaattg ttcctttgtt aaggttattg atcctagggg
    14701 aaaatttgga aaatatgata tttatgggga cagcagatat gaattagcaa aactatttca
    14761 tagtttagat ggtaaatatg attatatcat caaggatcta tttactgtag aatatgatac
    14821 cgagtgtgca gtgatagatt atacaattaa tacgcgtgta agagattacg atttatttga
    14881 attgtttaag tcggtctttt cggacgagat taaggacgat tttgataaga ttgtattgat
    14941 agaagcgttg ctatttctta gtatgattcc attacacggt gaaagttttg atcaccaaat
    15001 agtgatgctt gctacaggta tggaacttct tgcaagagta agtaataaag ttatactcta
    15061 atataaattg tgccttaatg aataacggtt acaatactgt tttagcaatg taggcattaa
    15121 ggcacgatta tattaattaa tacaatagaa gggaagagaa aaatgtatag tgaatattct
    15181 tttgtgtttg atatcgatgg cactttgtgt ccgataaaga agaaggatga gcgatatgag
    15241 gatttggtgc cgtatgagaa tatggttgaa aaactgagat tctataagga gaatggtgct
    15301 aaaattacat tgttcacctc acgcaatgtt aattcctata aaggaaattt gggaatgata
    15361 aatgctaata ctgcaaaagt gctcttggcg tggttggata aatgggacat tccgtatgat
    15421 gaaatcctgt atggtaagcc ttggccgggg cataaaggct tctatgtaga tgatcgaacg
    15481 gttagacctg atgagttttt gagatgctct gtagatgaat taaatgaaat ctgcaacagt
    15541 tcaaaaaaaa gattctaaat aaggaggtta gttctatgga aaaaaagatg acagttatca
    15601 ttacgatggc aggtttggga agtcgttttc gaaaaattgg ttatgcggtt cctaaatata
    15661 tgattaatgc caaaggaaga actttatttg attgggccat ggatagtttg gttggataca
    15721 ataaaaatgt tgataagtat atttttgttg ttcgtcagga agacaacgct tcagctttta
    15781 taaaggagca ttgtgcttac tttggtattg aaaacgtaga agtgattgaa atagactata
    15841 tgacagatgg tcaagcaact acatgtatgt tggctattcc ttattgcaat caagacaatg
    15901 agattatggt ttacaatatt gatacatacg tggaatcaaa tgagatgaaa tacgaagata
    15961 tttgtggaga tggacatatt ccatgctttc atgctgatgg cgatcattgg agttttgcaa
    16021 agcttaacga tcgaaatgaa gtaatagaag tccgcgaaaa agttcgcatt tctgataatt
    16081 gtactcttgg tgcgtattat ttctcatcgg caaagctgta taaagcttta tatgaagagt
    16141 attatgcaga ctcctctaat atggagaaga atgaaaaata tatagcacca ttatataact
    16201 ttatgataaa aaaagggtta aaggtgacta ttagcatagt agatgcgaaa aaggttcatg
    16261 tgcttggtac tccagaagag ctcaatgtgt ttttgaatca agaataaata gtgtttagga
    16321 gaaaatagca gtgaccagta ataatctaat tgtaaaaaca ttaagcagat ataagaatac
    16381 tccacttcct gttaaggctg cattatggtt tatggtatgt ggttttttac agagaggggt
    16441 ttcaatgatt acaaccccaa tttttactag agttttgtct acagaacaat acggtttata
    16501 cagcacttat ctatcgtggg aatccttatt gctcatgatt acatcgctta gtttgttcaa
    16561 agccatgatg aatctttatg taaaatatga taatcaagaa catattcttt cgacagtatc
    16621 cggattgact ttagtactga ctattttttg gtttgttatt gctattttta ttaaggatat
    16681 attggcagaa attttaggat tgacaccagt tctagttgtc tgtatgttct catattttat
    16741 atttcaggca gaaatacaat gttggtcttt acacaaacga tatatatatg attataagac
    16801 tttattagta gctacccttg cagttactgt tggctcatct gtagttggct gtttagcggt
    16861 tctgtttata tctcctaccg ctgaggcgag agccttaatg aatgtcctag tgactggtgt
    16921 tgttggcgtg gctgtgtata tatcggtatt tagaagatgt aaggatttta aggataatga
    16981 agtgtggaat ttcgcagtag tattttgtgt atcgttgatg ccacactatt tatctgaatt
    17041 tattctgaat ggttctgata aaattatgat aaattactta tgtggttcgt cagatgttgc
    17101 tatttacagc gtggcatatt ctattggtgc tctaattaac cttgttacaa attctgtcaa
    17161 ttccactttt gcaccttatc agtatcaaaa aatcaagtca ggagaatatg atttactatc
    17221 aaaaagagct aatgaagttc ttggtttggt agcggctatg ctttttgcat taatgctatt
    17281 tagtaaagaa attgtgttga tatttggtgg gcagaaatat atcgacagtg ttaatttaat
    17341 tgtacctata tgtatcggta tttatttcaa ctatatgttt cagctgtttg ctagagtaca
    17401 ggaatacttt gaaaagaaac tcactgtcgt tattccatcg gttttgtgtg caattatgaa
    17461 tattgtacta aattatgttt ttataaagtt atatgggtat caggcggtag catacacaac
    17521 tgtattctca tatatgtttt tctgcgtcat ccattatatt ttttatagaa aagtttgcaa
    17581 agaactattg gatggaaaaa acatatatga tgtcagagga atacttttga tttctgcaat
    17641 cacgatagct ggtggcatcc taatttcaat aattaataaa tttgatttca taaaatattt
    17701 aattatagca attgcttgtg tcgctgcaat tatatttaga aaacgattgc tacttttagt
    17761 aaggagaatt atgagcaaat aaacatttgc tcatagagag atagagaatg atagatatta
    17821 tttctgctat aatggtaaat gtattaacag cattatatca gccgttctta tttgccgtat
    17881 taacagcgta tctgtttatg tactggtatc tatttatagt tgataactct cgagatccta
    17941 aaaacttaaa agattctgta ttaatctggt tgagatattt taaagcatct agtaaatttc
    18001 gcagactctt ttttcttgcg ttttatttta ctttgatatt atttaaaacc ttgcttaatc
    18061 gaaatatgtg gatgaacccg ctttcaaacg ttattggaca gtggtcatta tatattatag
    18121 ataatgctac aggagagagc agattgacaa cagaatgcgt ggagaacacg atgctgttta
    18181 tccctttttg catgttattg ttatggtggc gtgacattaa atgtgatatt atacgcacca
    18241 tctatgttgg gattaagtat gtgtttttat tctcattaag tatagaattt acacaactgt
    18301 tttttaggct tggtacattt caactatcgg atttgtttta taacacccta gggggcttaa
    18361 ttggtgcttt gctatattgg ctattctacc gattaaataa gtatatagac ttaaaataga
    18421 actgctttga attttaatat ggttaatatt tttctgcaga caggattgtg taatctcatc
    18481 aagaatactt tacatgccat ctatttttgc taaaacattg ttgttatatc ttcaagaggt
    18541 agggaatttt attgctttaa atgaggtcga gcgaattaaa aaattggtaa aatatataac
    18601 atggtggtag ttatggtaaa agtgtttgat ataattaagt tttcggattt cagaggatat
    18661 actgctgttg cattggataa tgagactctt ttctcgattg gtattgagtt tcacattaag
    18721 caaataaatc aaggatttag tcataataaa tatactatgc gagggctaca ttatcagagt
    18781 ggagagcatg ctcaaggaaa gatagtgatg tgcctacatg gtgaaattat gaatatcgca
    18841 cttgatattc gacaaggatc ttccacttat ggatgtgtgt atgtcaacac attgagtgaa
    18901 agcaatggta aatgtgcgta tatcccacgt ggatatgctc atggctatct tactatggag
    18961 gacaatacgc tagttcagtg gtgcgtagat aatgatttca attctaatgc tgccaaatgt
    19021 ataagttatg aaagcgtaga tattgaatgg ccggtcagtc ggcagtgttt cgttataagt
    19081 gagaaagaca aaaatgggga aagattaagc ttttgattaa aatgagctgt ggagaaaaga
    19141 aggatactgg aaaataagaa gaacaataac cgagcaaatg ctcggttatt tctatgagga
    19201 atacataaaa ctaaatttag atatagatat ttattcacga aaaactaata tt
//
LOCUS       DACEJZ010000059        18939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEJZ010000059 DACEJZ010000000
VERSION     DACEJZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.061"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..1040)
                     /locus_tag="IW656_001854"
     CDS             complement(21..1040)
                     /locus_tag="IW656_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0837562.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1062..2069)
                     /locus_tag="IW656_001855"
     CDS             complement(1062..2069)
                     /locus_tag="IW656_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0837563.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2062..2727)
                     /gene="pgmB"
                     /locus_tag="IW656_001856"
     CDS             complement(2062..2727)
                     /gene="pgmB"
                     /locus_tag="IW656_001856"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0837564.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2720..5014)
                     /locus_tag="IW656_001857"
     CDS             complement(2720..5014)
                     /locus_tag="IW656_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0837565.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5274..7439
                     /locus_tag="IW656_001858"
     CDS             5274..7439
                     /locus_tag="IW656_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837566.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7743..8576
                     /locus_tag="IW656_001859"
     CDS             7743..8576
                     /locus_tag="IW656_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0837567.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8678..10378)
                     /locus_tag="IW656_001860"
     CDS             complement(8678..10378)
                     /locus_tag="IW656_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837568.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10885..12045
                     /locus_tag="IW656_001861"
     CDS             10885..12045
                     /locus_tag="IW656_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0837569.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12061..12504
                     /locus_tag="IW656_001862"
     CDS             12061..12504
                     /locus_tag="IW656_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0837570.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(12987..13517)
                     /locus_tag="IW656_001863"
     CDS             complement(12987..13517)
                     /locus_tag="IW656_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0837571.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13622..14173
                     /locus_tag="IW656_001864"
     CDS             13622..14173
                     /locus_tag="IW656_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0837572.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14326..14799)
                     /locus_tag="IW656_001865"
     CDS             complement(14326..14799)
                     /locus_tag="IW656_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0837573.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            14937..16409
                     /locus_tag="IW656_001866"
     CDS             14937..16409
                     /locus_tag="IW656_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0837574.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16406..17116
                     /locus_tag="IW656_001867"
     CDS             16406..17116
                     /locus_tag="IW656_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0837575.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(17395..18687)
                     /locus_tag="IW656_001868"
     CDS             complement(17395..18687)
                     /locus_tag="IW656_001868"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0837576.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         5842 a         3457 c         3844 g         5796 t
ORIGIN      
        1 gtatttagtg agtcagctat ttagtatggg ttttctaaag gaacgactgt ctctcttttg
       61 atcaagcgat gagggatgac caaacgtacg ccactgctca attcttcatg gatcaattcg
      121 cgcagctttt gcatagcgac acgtcctaat tcggagatat cgatatcgat acttgtaagg
      181 taagggtgtg ttaatgttga gaagatcgag ttgttgaagc taatgacgga gagtgtctct
      241 ggaatgacat agccatacat ttgcgccaac tgcatcgtgc gaagggcgaa gatatcgtca
      301 atgaccacca atgctgtggc ttcgttttct ttcaacgttt tctcaaagga cagataatcc
      361 tccggatgct ctagtactac actggataac gcaggaagac cagccatcat cattgctttt
      421 tgataaccga aatatcgttc gaaatacaaa ctttcatgag tcgtgtttgt cacaaacagg
      481 atattcttat gtccattctg gatcaaaaat tctgttgctt gtttacctag aagctggtta
      541 tcattatcta cataaacgat ttcgttctca tgatgataag gctgtccgat caaagtgaaa
      601 gggatgtggt tttggtataa ataatcaatg acagtatctt gactatctga atacactaga
      661 ataaaaccat ctacttgttt ttgtaaatgc atacgctgga cgttctctaa cagcaattca
      721 aaatttttag cagtagcgat tgctgtagtc atatcataat tccgcgcttc ttcgttgatt
      781 gcttccatga tctcaagata aaatgggtta ccgatccgct cttttgaatc aagagggggt
      841 aagattacgc cgatggcact ggaaatgcgt tttcctagat tgcgggcagt catgttagga
      901 acataaccta gttcatccat aatttttcgg actttcttct ttgttgtctc ggaaatacta
      961 ggatgatcgt tgatgacacg agagacggtc gaggtggcaa caccggcttt tttcgccaca
     1021 tcttttacgg taatagccat gataaaaaca cctttctctt gttattgtgc agagaaccga
     1081 taaatcgtct gactgatccg ttcttgacca ggatggaggt tgatcgatcc aaatttgggg
     1141 aaatgaacga tatcagggat ttcttgcggt tcgattgcta atccgtagtt tgaatgcatc
     1201 tgttttccat tgacagagaa atcagcttcg aatccagtcg tagaaaataa taccatactt
     1261 ttattagaag ttgctatagt catttggcga ccagaatgct tctcgaataa ttgtaaggaa
     1321 gttttggaaa gcctaggatg atgcaaaaga aatacatcat ctagtccatc tggataacat
     1381 gcaaggatct cttgcagtgt tggggatttt ctaaagtcga ttggcagttt ttcttttttt
     1441 gtccaatttc cagtaggcag tttttcttga tctaattcaa gcacaccatc taaaaagacc
     1501 gttaactgat gttcatagat gtctcttgtt ccatttcctg aaaggttgaa gtaggcatgg
     1561 ttggtcggat tgcagatggt ttcaacaagg ctactagcag aggttgtcat ctctaattcg
     1621 ttttttgtta aacggtaagt gatcgtagca gtgattggtc ctggataggt agaaaattca
     1681 tcttttaagt aaaagaccac acctattgat gatggagttt tgaaaacttc tacttcccaa
     1741 aattggaaag accagccttt cgtgccaccg tggatatggt gtgatccgca gtttttctcc
     1801 aatctatgct caccccaaga agcatcccgg atccttccgg ctacaggtcc gacagttgct
     1861 ccaaaaaagc ttttgtctgt tagaacatct tcgaaacgat cgtaagacaa taaaacattt
     1921 tctgattttc cttctttgtc tggtgcgaag atttggtgca ggcgggcacc gtaattgaga
     1981 agaacgactt ctaagtagtc gttcttcaat gtgattttct ttatttcagt ctctggaact
     2041 ggtacgacgg agatctctgg attatccatg gtcatgccac ttttctttca agtaatcgat
     2101 cgtaagaaaa gtcgtatcag ggacaagcgg gatctcctct ccaaggtctt cagcacgtcc
     2161 cacgccaatt ggttgagcgc cacttgagag gatggcttgg ataccggctt gcgcgtcttc
     2221 gatcccgata cattcttctg cttttagtcc gacagctttt gctgcaagga ggaagatatc
     2281 tggcgcaggt tttccgtttg ttgcttctgc cgggtcggca atagcatcaa agtaaggtgt
     2341 aagacccatt ttttcaagta ggaatggccc gtttttactt gcagaagcaa gagcaatctt
     2401 gattttttct tcttttaaag aatccagtaa aggcacgacc ccggggaaga catcttttgg
     2461 ttcgatcgtc tggatcatct ctaaataata atcgtttttt cttttcgcta attctgcaaa
     2521 ttcttccttt gaaaaagagt gttcttttcc gccataagct aaaagcaaag agagtgagtc
     2581 ttctcggctg acgcctttta gctgttcgtt gaatgtgtgg tcgatcgtaa tgccgatttc
     2641 ctgaccaagc tttcgccaag cacgataatg gaattcagca gtatcggtaa tgacaccgtc
     2701 taaatcaaac aatacccctt taaacatttg cttcctcctt tcttagcgtt atttctagac
     2761 gatccgctaa agaataagac tgttcataga cgtaaatttc tagaggagaa ccttctaata
     2821 actcgaaagt aacttgttca tctgtgactg agatgaacaa taaacgtccg cgataattga
     2881 tatggaaaga ataagctttc caagtttttg gcagaaatgg tgcgaatctc aactgatcat
     2941 gggcagtttt catttgagca aatccttgga caatcgacaa ccagctacca gtcatggaag
     3001 tgatgtgcaa gccgtcttct gtgtcgttat tgtaattatc caaatccaag cgagctgttc
     3061 gctggtacat ttctacagct ttgctttcca ttcctaattc tgctgctaaa atcgaatgga
     3121 tacttggcga aagggaagac tcatggacag tcattggttc atagaaatca aagttttttt
     3181 gcttttcttc ttttgtaaaa gtatcattga agaaataaat cccctgtagt acatctgcct
     3241 gcttgatgaa gcaagatcta aggattttgt cccaagacca atgctgattc agaggaagat
     3301 cgcttgtggg aatatcggat acaggcatga gatctttatc taagaatgtg tcatgctgga
     3361 caaagattcc taattcttcg tcaactggga agtacatgtt ctctgcgata tctttccatt
     3421 tggctagttc ttcttcgctg atagtgatag cagcttcttt ttgataagtt aaataatttt
     3481 ctcgcgtgta acgcaagacc caagctgcga tcgtatttgt ataccaattg ttattgatat
     3541 tgttctcata ttcgtttggg ccggtcacac cgtggatcat gtatttattt gctcgttttg
     3601 aaaaatggac acggtctgcc cagaagcgtg cgatttctgc taaaacttcc agtccttcat
     3661 ttttcaaata ttccgtatcg ccggtgtaat tcgtataatt gtagattgca taggcaatcg
     3721 cgccatttcg gtgaatctcc tcgaatgtga tttcccactc gttatggcat tcgacaccag
     3781 tgaaggtgac cataggataa agcgcgcctt ttaatccttg ttgctgagcg ttgtggattg
     3841 cttgcggcag ttgattatgc cgatatttca gcagattctt agttacttca ggtttggcta
     3901 aagcaagata aagagggaca gcatacgctt ctgtatccca gtaagtggct ccgccgtatt
     3961 tttctcctgt gaatcctttg gggccgatat tcaatcgttc atcttcgccg tagtaagtag
     4021 agaatagttg gaacaaattg aatcgaatac cttgttgtgc ttcttcgtct ccttcgatga
     4081 ccacatcggc aagcttccat cgttctgccc aagccgctgt ttgctcttct tttgcttgga
     4141 tataattgtt tgtctggttc gttaattctg agacagcttt ttctgcttgc tgcgcttctg
     4201 gaatatctct actagtaaga acgataattt ctttttccac tgaccatgtc tcaccttgag
     4261 ataattctgc agacaagcaa ccatgtaatg ctaaagtatc ttcgtgatag tcagggatga
     4321 ctgattttcc attaacgaaa tggcgcattg ctgcagtaac agtaaattgt tcaatcccaa
     4381 agttgtttgg aatagtttta gtcgtcaaat agccgatttc ttctatagtt ccacgatcaa
     4441 tctcattcca aaaatgttca tcatagttgc tgtcttcgtt ttgaacattt ccatccaatt
     4501 ttgatacgag tttgactgtt ccgctacctt caagcatttc tatagttaaa tagatataag
     4561 ctgattcttt tttgacaatg ctcagaaaac gttcgaagga aatgcgtact ttattagtag
     4621 aagtagtgat tgtgaatgta cgagataata caccattttt catatctaat tcccagtaaa
     4681 agtcttctgg ttccaaagaa gccaagtcga tcagctgatc gttgatcgaa atatctatcg
     4741 cgataaagtt caaggcgttg atcacttttc caaagtattc tggataacca tttttccacc
     4801 agccgacacg tgtcttatcg ggataccaga cacccgccaa ataagtacct tgatggtggt
     4861 cccctgagta agtttcttca aagttcccgc gcatccccat ataaccgttt ccaatacttg
     4921 ttaaagattc ttgcagtcga agattttttt tatctaattg tgtcgtgctg attttccatg
     4981 gatcaatagc aaatagtcgt ttgatctgtt tcatttttct cctcctaatg agctgtttta
     5041 tacgttcata ataaacgcaa ccgattgcac aagtcaatag acaaaagaaa atatttcata
     5101 gaaaaaagct taaaaaaata aatttttagg agaaaattat agaagggaac acacgtatta
     5161 taggttttat agaatggtat agacagaaaa ataaaaaata aagcatttac aaaaaagaaa
     5221 acgcacgcta taatacgttt aacgaaaccg attgcgtaaa ggagctaaac ggaatgaaaa
     5281 agatatttag ttttgagttt tggcaaaaat ttggaaaggc tttaatggtt gtggtagcag
     5341 ttatgccggc agctgggttg atgatcagta tagggaaatc aattcccttg atcaatcctg
     5401 attggacgcc gcttgtcaca acgggcggag taattgaaaa tattggttgg gcaattattg
     5461 gtaatcttca tattttattt gctttagcaa taggaggaag ttgggcaaaa gaaagagcag
     5521 gcggagcatt tgcagctggg atctcattta ttttaatcaa tcggatcact ggggctatct
     5581 ttggtgtcac aagtgacatg cttgcgaatg aagatgcatt tacccacact ctgttcggta
     5641 cgaagatcat ggttaaagga ttttttacaa gtgtattgga agcaccagct ttgaatatgg
     5701 gagtattcgt agggatcatc gctggatttg tcggggcaat ggcttataat aagtactata
     5761 attatagaaa attgccggat gctttgtcat ttttcaatgg aaagcgcttt gttccgtttg
     5821 ttgttatctt atggtcaaca atcgtggcat tgattcttgc tgtggtgtgg ccaaacgtac
     5881 aggctggaat caataatttt ggtttatgga ttgcagaatc acaagaaagc gcaccgattc
     5941 tagcaccgtt tttatttgga acattagaac gtttactatt gccatttgga ttgcatcata
     6001 tgctgacgat tcctatcaac tatacacagc ttggcggatc gtatgagatt ttatcaggcg
     6061 cacaggcggg gacacaagta tttgggcaag atccgttatg gttggcttgg gccactgatt
     6121 tagtgaatct taaaggtgca ggggatatgt cgcaatatga gtttgtgttg cataactgga
     6181 cgccggctcg tttcaaagta ggacaaatga ttggttcttc aggtatcttg atgggaatgg
     6241 cttttgcgat gtatcgtaat gtcgatcctg ataagaaagc aagatataaa tcaatgtatt
     6301 tttcagcagc gttggctgtc tttctaacag gtgtaacaga accattggag ttcatgttta
     6361 tgtttgctgc tgtgccatta tatgtcattt attcagtgat ccaaggggct gcatttgcga
     6421 tggcagatat cttgccattg cgggtccatt catttgggaa tatcgaattg ctgactcgga
     6481 caccgttagc tatcaaagcg ggtctaggtg gagatcttat caacttcgtg ttgtgtgtga
     6541 tcgcttttgg tgttgttacg tatttcttag caaatttctt gatcaaaaaa ttcaactttg
     6601 ctacaccggg acgtaacgga aattacgaca atgacagtga agaaaccgtt gtatctaaca
     6661 gcggaacagg aacagcagat cagcaagtgg tccagatcat tcatttgctg ggaggaaaag
     6721 aaaatatcaa agatgtcgat gcttgtatga ctcggttacg cgtaagtgta aatgatcgag
     6781 aaaaagtagg gactgaagaa gcctggaaac gagcaggggc gatgggattg atcgtcaaag
     6841 acaatggagt ccaagcagtt tatggaccaa aagcagatgt cttgaaatca gatatcgaag
     6901 atctgctgca atcaggagca gagattccag agccagaaat ggtttctaca ggaaatggac
     6961 aagcaggtag taagcaatac ttaggtatag aacaagaact agtttccgca gcttctggag
     7021 aagtgattcc tttatctgaa gtaaaagatc cggtattctc acaaaaaatg atgggtgacg
     7081 gctttgctgt tattccaaca tcaagagaag ttgttgcgcc gatcgctgga aaagtgacaa
     7141 gtattttccc tagcaaacat gcaattgggc tggaaacgaa agatggtatc gaagttttga
     7201 tccatatggg aatcgataca gtccaaatga atcagccagc ttttgagatt gctgtcaaag
     7261 aaggccaaga agtaggaaca ggaacgaaat tagctgagat ggatcttgat gtgatccaaa
     7321 acgaagaaaa agatccaacg atcatgatcg tatttacaaa cgataaagta gaagaagtag
     7381 tgatcaagca attaggtaca gtgacagctg gtaaagtgat cggctccatt aaaatgtaag
     7441 gagaggttgt gaaaaagctg tcctgcatca agtcataaga acagccaaac aaaaatagct
     7501 cctcctattt ttcgtttggt tgggcttatt accgagaagc agcagtttga atactgttta
     7561 gtggagaggt cgtgaaaaag ctgccctgca ccaagtgata aaaacagcca aacaaaaaat
     7621 agctcctggt ccattgagct tattactgag gagttagcag gagaacacta tttgctggag
     7681 aggttgtgac aaaaattttg tcacaaccct tttgtttcga aatgaagaca aggagaaaac
     7741 caatgaaatt catgacactc aacacgcata gctggcttga gccagaacca gagaaaaaat
     7801 tgcaagaatt agcagataag atcctgttag aagattatga agtgattgct ttacaagaaa
     7861 tcaaccaatt actggaatct gaagaagtag aacctgcgaa gctaatgaag ttctgtccgg
     7921 taaagaatca agtacctatc cgtaaagata attttgccta ccgtttagtt cagcttttaa
     7981 aggaacacgg aaaagagtat ttttggagtt gggaaatgag ccacatcggt tatgataaat
     8041 atgaagaagg aaatgcactg cttacgaaaa aaacattgga aagtcaggtc cttaccgtct
     8101 cccaatttca aaagaaagaa gattatcaga cacgcaagat cttgattggg aaaacaaaga
     8161 tagatgatca ggacattttt gtctcgagct gccacttttc ttggtggaca gataaaaaaa
     8221 gtggctttta ttttgagtgg aaaaacttgg aaaactactt tttggaaact cgcgtaccac
     8281 tttttttcct aggtgatttc aataatccag tagactctca aggttattat actgtaagag
     8341 aaagttgcct tcttcttcaa gattcgtatg ttgtcgcgaa tgaaaaagga aaagcagcta
     8401 ctgtggaaaa gaaaatagat ggttgggaac aaaatacaga aaaattgcgt atagatttta
     8461 ttttcgtccc tgaaggaatg caggttaaaa aatatcaacg gatttttgat gggatcgata
     8521 gtccaatcat cagtgatcac tacggggtgg aaatcgaatt agatgtgaac gaataaaaaa
     8581 tataacatat aacataaaaa aactgattaa aataaaactc tccaaacgat tataccgttt
     8641 ggagagtttt ttgatcggat gtatttatcc cttttgccta agcacgtaaa agcttgtatc
     8701 tatgataaat aaataaacat agcaaggtaa accagccaat caaagtaaga gaaagaatga
     8761 aatagaaata gattggcgtt tgataatgaa gcgtgagctc gttgattcct ttttgctgct
     8821 tcacgattgg ggtcccgata tctgttactt cataatcagt caatgttttt ccattttgct
     8881 gtagcacagt ccgatcgtag acgataacag ggatatgaac agttttgtcc ccagtgtttt
     8941 tccacttgat tgtcaaaaga ccgcctggat gtgtttttat aaaagttgtt tcgttttcta
     9001 gtatttcttc gttataccgg tcgtatttat tgtcttttgt ctccttatag attggcagat
     9061 agtcaggtgt ggttttttgg aaaacgaaca agctggtaga catatctttg ctgaagaatg
     9121 ttttccttgc ttcttccggc gtatcgaata gataagtatg tcgactgaca aatgtttcat
     9181 tttcccactt ttccagatgc tggtataagt tctggatcgt ctgtgctgaa aaaataagaa
     9241 tgccaaatat acttaaaaga cggaaccact tttgttcaga ccactggttc agtgaaaggg
     9301 caaggtaaag tagaagcaat agcgtaaatg gcacgaaaaa tcgaaatgga aactggatca
     9361 aactaataaa aggcacattt ttttcgatca atgtcgtcca tggaatcaag ctagtagaaa
     9421 gaaaaaagaa aacaagactt gtgtacaaca cgattttttg gaataaggtg ttcttttgtt
     9481 gaaagaaaat aaaaagcaaa aaacctgata atatcaatat aggaaaggcc ttagggtaat
     9541 ggagccaata actgccatcc agatctatcg ccttttgact catttctctg ttaacaaagg
     9601 gaggaagaat cttattccca ctgtaaacaa tagctaatcc tgcccaaata tttgctgtca
     9661 gaacaaaata aagcaagacc gacttaatca gttgaagcag tgcttttcct ttttcagtct
     9721 ggttaaaaaa gtaataaaga taaaaaggga tatacatcaa agctaacatg atcgaagaca
     9781 gtaaatggat ctgtgtcata agtcccactg agacagcgac tttgataaca gggaactttt
     9841 tatgaaggac gaaatcagca atagggatca tacataaagg ataaaaacaa gtcccccagc
     9901 tagtgaatcc ttggttgata gaccagtatt gaatagaata cgtactcatg aataaaacgg
     9961 ctactggtac acttacccaa tttcgaacat tgacatatct cagtaaaatc agcatagaaa
    10021 atcctgaaat aagataaaga atgaaatttg aaagaatctg ataatgaaac cagctacctg
    10081 caagcaacac gatcaatcct tgtaaataag cgaaaaatgg gccgtaaacg gcattcacga
    10141 tcctgccact ttgctggaat ccataaaggg aaggaaaata atggaaatcc ccatatttaa
    10201 tttgttgcgc ggcatcatag aaccgattgt aatggaatgg ggcatcagca cccaaaatta
    10261 gtttatgtgt gaataaatga ggcatgagaa atgccaaagc aatgaaaaaa attgctaaaa
    10321 atgggccata ttttttcaat tgtcgaactc ctttgcaaac tttagataaa ttcatcatat
    10381 atcctatgcg agaggtaggc aagaaattag aaaattcatg aaaatattta tgaattttac
    10441 aagaaaaaca taagtgaaag ttctaaggag agaggatgcg acataagttt tgttgcatct
    10501 ccttgttttg aaacagaatt tttcttaata ctcgaaacaa agtagctttt tcatgacctc
    10561 tcggtaaacg gtgttcaatc agctgcttct cggtaataag ctcactagcc tagaaaatat
    10621 gagaagctat tttctgacta gcgctcttat tactcaaagc ataacgcagt tttcacaacc
    10681 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac ccaacaaaaa
    10741 atatgagtag ctattttttt gggctgtact tattacttga tgcaggacag ctttttcaca
    10801 acctcatcgg ctttcatttt ctattttttt cctgaaccca tataataaaa gaaagaacaa
    10861 aaaaacttgc aagggaggac acatgtgaag cagagtaagc aaaaacagtc tcgtcgactt
    10921 ttgtttcgtt tgtttttatt aatatgtatc gtcgtagggt tactgatcgc tttatatcca
    10981 ttttatgtgg attctttaaa tagttttatt gatcaaaaac gtatcgaaca agtacaaaaa
    11041 cggaccgcag ctgaaattga agtgcagcga aagaaaatgg aggagaaaaa ccaacgatta
    11101 actgaccaag gattcaatcc tggtactgat cctttcaatg aaaagaacag aaatgaaagt
    11161 acaacatcca gccaattaga ggaatggctg atcggaagta tcaatatacc taagatccaa
    11221 ttaaatattt cactttatga tcggctcaat ggtaggatcc ttgaaaatgg agcaggcgtc
    11281 ttgcaaggaa cctctttccc gcttggtgga aattcgacgc attcagtgat ctctgctcac
    11341 agcggtttac ctaatcgacg gttatttacc gaattagatc gtttagaaaa cggagacacg
    11401 tttattttga ctgttttggg ggagaaattg gcttatcaag ttaaaaatat acaagtggta
    11461 ctgccagatg acacgtctgt tctaacgatt tcagaaggag aagacttagt taccttgctt
    11521 acatgtacgc cgtatatgat caacacgcat cgtttgctag tgacaggaca tcggattcct
    11581 tataacgaat cggtaaaaaa agaagaagga aaaggaaatc aggaaagaac catgcggcaa
    11641 tggtttattc ttgcaggaac gataatagct gtagtcatac tgttagtgtt tatcggacga
    11701 ctaatctatc agtatagatt aagtaaaaaa gtgcttgatt tttcatttat tattagtgat
    11761 tcgtcaggga aaccggtaaa tggtagttca ttcattttga aacataagaa aaaaacactt
    11821 actagaaatg gtgtgccgtt ttcagtacaa agcgaccatc atgggaaagt caagctggat
    11881 caattgcctg gtggaactta tcgaatcgtt tcagctgatg atcctaaagt tgctgtttct
    11941 tttggtatta gaaaagtaaa acaagagaag atatactttt ttgaggggcg aaaacttgta
    12001 aaagagcttc aaaaaaatgg tttttatttt aaaatcaatg aataagtaag gagagaaaaa
    12061 atgatccgca caacaacaaa attgtctgcc aaagaactgg aatctgttct atcgatttgg
    12121 ctgcatgcta acttggattc tcatgaattc attccatcaa cttattggga gaatcaagta
    12181 gaatctgtta ggaaagcttt accagatgca gtgatttact tagcagaaat agatcaagaa
    12241 attattggtt ttgctggggt gattgatcag tttatcgcag gaatctttgt gaaaagggaa
    12301 taccggcaac aaagaatcgg tacgcaacta atgaaaaaaa taaaagaaac caataaaaca
    12361 ctgactttga cagtctatca aaaaaatcaa tcagctattc atttctatct ttcacaaggt
    12421 tttacaattc aaacacagca aaaagatgaa gcagtaaatg aggaggaact tgtgatgaaa
    12481 tggaatcaaa acgctgattt ttgatgaaca gaataacgaa ataatgctcc cagcctaatt
    12541 ttttgctggg agcattattt cattttgaag tcactaattt aactgtgtga atatgcagtt
    12601 gttacaatct ttgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    12661 gcaaaatatg aggaactatt tttccatttg tttctctaat actcaggtca aaacgctgat
    12721 ttcacaacct cttgataaac ggtgttcaaa cggctgcttc tcggtaataa gcccactagc
    12781 ctagaaaata tgaaaagcta ttttctaact agcgttctta ttactcaaag cataacgctg
    12841 atttcacaac ctcttgataa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    12901 acggcaaaac atgaggaact atttttccgt ttctttctct aatactcagg tcagaacgct
    12961 gatttcacaa cctctctttc acaaccttat tttactcgtt tataattttc atcgaagtat
    13021 tttccgctac ggatatcatc tggcattcca tcgtatggtt cttcgctaat aacatcttca
    13081 ttgtctatac ctgcatcacc catataagtg atcgtagcaa attcaggaac aagtaatttt
    13141 ggataagttt catatttttc acgagctgct tccatttctg cgatatgttc gttttggaaa
    13201 gtaacagtga tttctggatg ttcttcaacc catttaggga aaaagatcat tccgtgaagt
    13261 ttcttttcat ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc tgtccaagtg
    13321 attttgaata cgccgtccgt caatttaacg atatcagctt cttggtcttt tacccaacgg
    13381 cctttgacca ttccgccatg gatacggtaa tcaacagtat ggtcattttt ggcataccat
    13441 tcatattccc aaccattgtc atacgtatag ataaagtgag ttccaataaa atcttccaat
    13501 tctttaaatt ctttcatgtt taagcatctc cttataaatt aaaacatgta ataaactaca
    13561 tgttttcgat tactcattta tgatagaatg gatttaaaga tttgtcaagg agggaaaact
    13621 gatggcaaga aaaaatacac ttcgatatat tttgttaggg ttattaagta agaagaagat
    13681 gacaggatat gaattgaatc aatcgttcaa aaatgaaata ggagaatttt ggcaagcaaa
    13741 acatagtcag atttatcctg aacttgcaaa aatggaagag caaggaatca ttcagcatca
    13801 agtagagatc acaggtgaaa aattggaaaa gaaagtttat gaattaacag aagaagggaa
    13861 ggaacaattg gcagagtgga ttcatacacc caccaatgaa ctaccggtta atcgagacga
    13921 atttgttctg aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag tagaaattat
    13981 tcagcagcag cgtgaccttc atgaagaaaa acgacttcat ctattagagc gaaaaaaaac
    14041 gatctttcct actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca
    14101 tgcgatcaat cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa
    14161 ccgaaaaaaa taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa
    14221 cgcagatcac cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta
    14281 gcatctactt attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa
    14341 caactgttcg tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt
    14401 ctcgttgtca tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa
    14461 aatcgtatag cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg
    14521 gacatggtgc attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa
    14581 tccgtcatct aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt
    14641 tctaggacaa ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct
    14701 taattgacgt ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg
    14761 atcattttcc aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct
    14821 cttgcttccc ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga
    14881 atttaggact aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga
    14941 gtaacaacca acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg
    15001 tcttattaat tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc
    15061 ctacattgat gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt
    15121 tcttgctagt caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt
    15181 caagaaattt atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta
    15241 cagcacctaa ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag
    15301 tatcgatgcc attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat
    15361 cagcaatggg gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat
    15421 ctggttatat cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg
    15481 ttgttcttgt cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc
    15541 ctagcattga tattttatcc gctattttat caacgattgg attcggtagt ttactttacg
    15601 gattttctag tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg
    15661 ttggtgtgat tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct
    15721 tggaacttcg tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa
    15781 caaatatggc gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg
    15841 gagaatcagc attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta
    15901 tgatgcctgt gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag
    15961 ggatgttcat tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag
    16021 ttttatatat cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc
    16081 cggtaacgac ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg
    16141 taaataatac attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt
    16201 tgacgaatgt gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag
    16261 cttataaaga tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag
    16321 cgatcatctt tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg
    16381 ttctaatgaa agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc
    16441 ttttgcttta acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt
    16501 cggactaatc ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg
    16561 aatggaaaaa gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg
    16621 agcagatatg cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg
    16681 aacaaatgaa aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt
    16741 agaaaagaca gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa
    16801 cgacatgtat aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt
    16861 gaatacattc aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt
    16921 agctgaatta gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca
    16981 agacggtatg gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca
    17041 acgtacgaaa gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga
    17101 agcaaaaggc gaataatatt ttgaacacca tgtaggaggt tgtgaaaaag ctgctctgca
    17161 tcaagcagta agaacaatca gataaaaata gcactccata tttttcatct gattgggctt
    17221 agtgccgtag atgcaagctt ttgaacaccg tttgttcgga aaaagggact gtgacacggt
    17281 ttttgtcaca gtcccttatt ctgaataaac agtgttcaag ttattattat cgtatagata
    17341 aacacttcga ctcgctctgg taagaacgtc ctgattcagt cgtcgttttt atccttagat
    17401 ttgcgcccag acgctttcta agatgtttgt ttgtgttcta tcaggtccga cagaaaatgt
    17461 tgagatacgt acaccaacaa gctctgagat acgacgtaca tagttacgtg cgttttctgg
    17521 cagttctgca agtgttttgc atcctgtaat gtcttctgac cagcctggca attcttcata
    17581 gattggtttg cagcggttca actctttcaa gcttgctgga tagtggtaga ttagttcgcc
    17641 atctaattca taagccgtac aaatttttac tgtttccaat ccacttaata catcgatcga
    17701 gtttaaagat aggttggtaa tacctgaaac tcgtttagaa tggcgcataa ctacagaatc
    17761 aaaccagcct acacgacgcg gacgaccagt tgtcgtaccg tattctcgtc ctacttcgcg
    17821 gatttgttct cctgtttcat caaataattc agttggaaat ggtccatcac cgacacgtga
    17881 cgtataggct ttgcagacgc cgacaacttt attgattttt gaaggcccta caccgcttcc
    17941 gattgttacc ccaccggcta ctgggttaga agaagtaaca aaaggataag ttccctggtc
    18001 gatatccagc attacacctt gtgcgccttc aaacaacacg cgtttgcctg catccaaagc
    18061 atcattcaag ataacggaag tatctgtgac gtattgtttg atttgttgtc cgtattcata
    18121 atattcttca aaaatatcgt caaaatcaag tggttctgaa tcaaacattt ttacaaattg
    18181 acggtttttg tcatctagat tcaaacgaag acgttcttca aaaatttctt tatctaataa
    18241 gtctgcaata cggataccta cacgagctgc tttatccata taggcaggtc cgattccttt
    18301 aatcgttgta ccaatcttat tttcgccctt agcatcttct tgtaattggt ccaatttgat
    18361 atgataaggc aagattacgt gcgcgcggtc agaaatacgc aaattacttg tttctacacc
    18421 tttttcatgt aaataagcta attctttaac caatgattta ggattgacta caacgccgtt
    18481 tccgatcaca ctgatcttgt ctttataaaa aatcccagaa ggaatcaaat gcagtttata
    18541 agtcacaccg ttaaacttaa ttgtgtgccc tgcattgtcg ccaccttggt accgtgcaat
    18601 cacttcagca ttttcactga gaaagtctgt gatttttcct ttcccttcat cgccccattg
    18661 cgtacctact actactacag atgacatgtg aacacctcgt tctatattat ttaatcctga
    18721 accattgtat caataataga tacatatttc aaataaaatc gaatgatttt tatttttatt
    18781 ttataaaaac gcttaaaaaa cgaactttat ctttttagaa tggttattaa aaaagaggtt
    18841 gtaacagaag tggtcagctt caagaaataa gaaggaatgt acgaaaattg cttctcaaat
    18901 ttttgtgaaa tttcgactta tttttcgaag aagctactt
//
LOCUS       DACEJZ010000060        18798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEJZ010000060 DACEJZ010000000
VERSION     DACEJZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.062"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            99..746
                     /locus_tag="IW656_001869"
     CDS             99..746
                     /locus_tag="IW656_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0837577.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IW656_001870"
     CDS             1139..3784
                     /locus_tag="IW656_001870"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0837578.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IW656_001871"
     CDS             4094..5407
                     /locus_tag="IW656_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0837579.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IW656_001872"
     CDS             5397..6050
                     /locus_tag="IW656_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0837580.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IW656_001873"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IW656_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ0837581.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IW656_001874"
     CDS             complement(6671..8023)
                     /locus_tag="IW656_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0837582.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IW656_001875"
     CDS             complement(8089..9378)
                     /locus_tag="IW656_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0837583.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IW656_001876"
     CDS             complement(9465..10169)
                     /locus_tag="IW656_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ0837584.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IW656_001877"
     CDS             10369..10635
                     /locus_tag="IW656_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837585.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IW656_001878"
     CDS             complement(10803..11033)
                     /locus_tag="IW656_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0837586.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IW656_001879"
     CDS             11105..11260
                     /locus_tag="IW656_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837587.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IW656_001880"
     CDS             complement(11569..11793)
                     /locus_tag="IW656_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0837588.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSPGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IW656_001881"
     CDS             12060..12395
                     /locus_tag="IW656_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837589.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IW656_001882"
     CDS             12628..13569
                     /locus_tag="IW656_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ0837590.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKGIK"
     gene            13571..14461
                     /locus_tag="IW656_001883"
     CDS             13571..14461
                     /locus_tag="IW656_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ0837591.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IW656_001884"
     CDS             14504..15304
                     /locus_tag="IW656_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0837592.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IW656_001885"
     CDS             15319..16170
                     /locus_tag="IW656_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0837593.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16167..16526
                     /locus_tag="IW656_001886"
     CDS             16167..16526
                     /locus_tag="IW656_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0837594.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16541..16702
                     /locus_tag="IW656_001887"
     CDS             16541..16702
                     /locus_tag="IW656_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0837595.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16699..17025
                     /locus_tag="IW656_001888"
     CDS             16699..17025
                     /locus_tag="IW656_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837596.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17022..17837
                     /locus_tag="IW656_001889"
     CDS             17022..17837
                     /locus_tag="IW656_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ0837597.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17976..18287
                     /locus_tag="IW656_001890"
     CDS             17976..18287
                     /locus_tag="IW656_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837598.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18284..18514
                     /locus_tag="IW656_001891"
     CDS             18284..18514
                     /locus_tag="IW656_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837599.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18521..18724
                     /locus_tag="IW656_001892"
     CDS             18521..18724
                     /locus_tag="IW656_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ0837600.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
BASE COUNT         6337 a         3115 c         3822 g         5524 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ccgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgg gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaaac ggacgaaaaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
    13381 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
    13441 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaagggg
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
    16201 gaggtatcaa gtttgattct atcgcggaag cagagtatta cgatctagcc ttgtggcaag
    16261 ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt tgagctgatg ccgaagtttg
    16321 aactagaggg caagaagtat cgcaagatcg agtatattcc cgacttcaca ttttacaaaa
    16381 gcggcaagct ggtcaaagtc gtagatgtta aggggatgca gacaaaagac tttaagatca
    16441 aggcaaagtt gttctgtcat caatatcaag tgccgttgat attagccaaa aaatatcgga
    16501 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
    16561 tcaaatgcga attcgaagcc ttcggcgtga gattgacgat ctggaacgaa caaaggcagt
    16621 gatggtcaat gaaacggcta gaaaggcaat cgatttgcac atagagaatt taagaaggga
    16681 aatccgtaga ttggaggaat gagcgtggat aaggaagcgg cttggcgaaa attaatgttg
    16741 ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt tcagcgtcta
    16801 ggaaagattg ctaacggacg tatacgaaaa aagccagaca aaagaaaaca gcgcaaaggg
    16861 aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc aaggaacagc tagtgagctg
    16921 tctgcagaaa ctggatatac gcgtgggact attcgaacgt acgcttggcg aaatcacacc
    16981 gatcgaaaag ggcacgaata taagtatttg gaggaagaaa aatgaacgaa aacaaattaa
    17041 tcaaattggg tgtagcagga gcagtaatcg taggtattgg agttatcgga ggatttaagt
    17101 tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta ttcaatgaac ggcggtatca
    17161 aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat tgacaaagtg atccaatatc
    17221 caattcgctt gcaaactatc caatcaaaaa atatttcagt atctacaagc gacggcaaaa
    17281 agacaacgat tgatatcaaa tatgactaca aagttgattc aactaaagca gcaaaaatgt
    17341 acaaggaatt tgggaatatc acttcggaag atatcgaaag tggatggtta aaatctaagc
    17401 ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct gcttgatgtc ctttcaggag
    17461 attcctctaa agttgaagct gaggtattaa cgaactttgc taaatcagtt gaatctaaag
    17521 ggtttgaagt cgaagacgta acacttggtg ttccagatgt cgataaagaa acacaaaaat
    17581 caatcgatgc gatcattcga gctggtcaag aaaatgaaaa agcgaagcta gacgcagaaa
    17641 ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac aaaagctgca gaagcagagg
    17701 cagaatctaa tcgcaaagtc gccgaatcag taacagacaa tttgattcgt tatgaagaag
    17761 ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg agcagatact gtagttacgg
    17821 atgaagcagg caaataatat gggattcttt atggctaaaa ttctcttgtt cttaggttta
    17881 gtaggagcag catatctcgt gtatgcgctc ctttcccaaa ctgatgacaa agaagatgac
    17941 aacaacgatg aaatgaaatt ttaggaggag aaataatgga cgaactaatc acaaaagtag
    18001 agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag tttttgaaag
    18061 tagctgagga attcggggaa atagcttcgg cgatggcaag aagtaatgat gaactattta
    18121 aagatagcgt aggagacgtt atcgtcacgc tgattatcct ttccatgcaa aaagggacaa
    18181 acgtacaaga gtgtttagaa atggcataca acgaaatcaa aggacgcaca gggaaaatgg
    18241 tagatggtgt attcgtgaag tcgagtgatt tggaggatgt gaaatgaaat acgaaatacc
    18301 actaagtgaa gcgggcattc aagcaattat caatggtcgg gaggttaaca tagaacttcc
    18361 tgatggtact gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt
    18421 aattgatcgt tttaacgtga ctgattctgt ggtagagaac cacttaaaag aatttcgatc
    18481 aagtatagac aacactttca gattagggag ttgattgaca atgaacacca gacaccgcag
    18541 agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag acaaagtttg aaaaagaata
    18601 tggaatttca gcagaagaaa catataaagt ggcaagtcag tttgttgctg atgcaagtga
    18661 tgctattcgt aagtttggga tttcgatatt aaatgatgat cgtaaatggg aggaaatgag
    18721 atgaaactaa aagacggatt ttacgctagt agtcacggca tcggcggttt aatgctagat
    18781 atgccgacaa agaaccct
//
LOCUS       DACEJZ010000061        18605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEJZ010000061 DACEJZ010000000
VERSION     DACEJZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.063"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(397..963)
                     /locus_tag="IW656_001893"
     CDS             complement(397..963)
                     /locus_tag="IW656_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0837601.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(963..1919)
                     /locus_tag="IW656_001894"
     CDS             complement(963..1919)
                     /locus_tag="IW656_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0837602.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1919..2575)
                     /locus_tag="IW656_001895"
     CDS             complement(1919..2575)
                     /locus_tag="IW656_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0837603.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2945..4963
                     /locus_tag="IW656_001896"
     CDS             2945..4963
                     /locus_tag="IW656_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0837604.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5390..7804
                     /locus_tag="IW656_001897"
     CDS             5390..7804
                     /locus_tag="IW656_001897"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0837605.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8152..9705
                     /locus_tag="IW656_001898"
     CDS             8152..9705
                     /locus_tag="IW656_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0837606.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10086..11870)
                     /locus_tag="IW656_001899"
     CDS             complement(10086..11870)
                     /locus_tag="IW656_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837607.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11885..13606)
                     /locus_tag="IW656_001900"
     CDS             complement(11885..13606)
                     /locus_tag="IW656_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837608.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13706..14467
                     /locus_tag="IW656_001901"
     CDS             13706..14467
                     /locus_tag="IW656_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0837609.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQFLLTNTDENNIRTEYYHAWIEMKK"
     gene            15434..15702
                     /locus_tag="IW656_001902"
                     /pseudo
     CDS             15434..15702
                     /locus_tag="IW656_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16374..>18605
                     /locus_tag="IW656_001903"
     CDS             16374..>18605
                     /locus_tag="IW656_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837610.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6114 a         3302 c         3755 g         5434 t
ORIGIN      
        1 gttgtaaagc gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc
       61 tattgatata tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat
      121 gtttttgcca ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt
      181 gtatttgaat aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
      241 caaaaacagc ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt
      301 gagcaccgtg aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa
      361 acgcaaaaca tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata
      421 taaaataggc ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc
      481 ctgtggcagt ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg
      541 accatagtag acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc
      601 catagctgtg cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata
      661 ccctagattg aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt
      721 ttcgtggcct tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc
      781 acgagtagca tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc
      841 gatgtgttct gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg
      901 atcccctggt tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa
      961 catcagacgc tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga
     1021 agttcttcat caattgcatt caagacttcc cattttttca agctccacat tggtccaatc
     1081 aaggaatccc acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt
     1141 tccagctggt cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct
     1201 tcatgatagt cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt
     1261 ccttggatat ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt
     1321 tcgcctggta atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta
     1381 gccacgccat ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct
     1441 tcaaaagttg tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca
     1501 aggtaatcaa ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg
     1561 acacctggta aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg
     1621 gtgtttgtaa aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc
     1681 atcatgtcga tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg
     1741 tctcccgaac cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga
     1801 ttcggacagt caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt
     1861 cgtaaagcgt aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt
     1921 aaaagtcctt ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat
     1981 aagtcaggca cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat
     2041 gaacgcccag ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt
     2101 gaagcaatgt gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac
     2161 cgtaaagaat catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta
     2221 ctaagtgcgc caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga
     2281 ttccagcaat tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat
     2341 accacatcca taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt
     2401 tagtcataaa gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa
     2461 accactgatc gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga
     2521 aaaacaggac aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat
     2581 tcctcctttg aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag
     2641 agataaaacc aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact
     2701 gtaatcggaa agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt
     2761 ttttcgaaat ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag
     2821 ttgttcatta cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt
     2881 ttaataaata ttaagagaag ttataattat taataataga taaattgaag ttggaggata
     2941 gaagatggaa aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg
     3001 aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc
     3061 cccattagta cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag
     3121 aatcaataca gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc
     3181 caacgatcta tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag
     3241 tagtgcgtta tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg
     3301 acaaaccgtc tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga
     3361 gcctttccga caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag
     3421 aaatacttca tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc
     3481 ctatacagat gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg
     3541 caaattaaat aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg
     3601 aaacgcaggc ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg
     3661 cggttcaagc aattctggta atacaaactc aggttcagca acttcgacca cttatactgt
     3721 ccaatctggt gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt
     3781 gatccaatgg aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa
     3841 aggtagccag gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg
     3901 aaatgctggt acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg
     3961 tgattctgtt tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg
     4021 gaacaatatc aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg
     4081 ttcttctaat gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc
     4141 gtccagctca agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga
     4201 ctctgtttgg agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa
     4261 caatatccaa aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc
     4321 aacaaacagc acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt
     4381 taatactggc agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg
     4441 gagcgtggca aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa
     4501 aaacaacttc atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac
     4561 aaatacaggt tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac
     4621 aagtacatca tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag
     4681 cgtagcgaac aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     4741 taatttcatc tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa
     4801 ccaacaatca gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag
     4861 tgttgctgac agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa
     4921 caactttatt tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa
     4981 ataacgattg aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac
     5041 ttgaaaagga agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg
     5101 cacggatgct tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg
     5161 gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg
     5221 cgtagattac gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg
     5281 aagtttgaga aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt
     5341 tatatttata atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa
     5401 tcataaagaa attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac
     5461 ccatgatgat cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg
     5521 tcaagggcta catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt
     5581 gaaaagaagt caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc
     5641 tgctgaacaa tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat
     5701 cgaaacattc cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt
     5761 gaatacaact gatccggagt attataaatg gacacaatgg atctttacga agctttatga
     5821 aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt
     5881 tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag
     5941 aaaaccaatg cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga
     6001 tctagaatta gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg
     6061 ttcagtagga gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt
     6121 tacgacacga ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga
     6181 tcttgttaga gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga
     6241 gacagctaaa aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt
     6301 tactggtgct tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga
     6361 ttatgtatta tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg
     6421 tgactttgaa ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga
     6481 tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg
     6541 catgaacaaa caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg
     6601 gaaaaaagaa gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg
     6661 tgaaccaatc ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga
     6721 gctaccatta cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc
     6781 attagcaaat atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg
     6841 tcgcgaaacg aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat
     6901 cgaccctcat aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt
     6961 tgacatctac attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg
     7021 gcataaattc ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata
     7081 caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt
     7141 agtcaatcct gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat
     7201 gttcatgggg ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg
     7261 taaattcttg gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg
     7321 tatcactaca ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt
     7381 gacagaagac tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat
     7441 caatgaagcc tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact
     7501 actcgcaccg attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg
     7561 tggtatttct tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt
     7621 agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt
     7681 aggaaaagat gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga
     7741 aggcaaaaca gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa
     7801 ttaattggct ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa
     7861 tgaacttcat tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa
     7921 aggttttgat tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt
     7981 aagagatctc tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa
     8041 actacctgaa attgagagta cataccaaat tcataaattt tttattagtg ggactagtga
     8101 ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa
     8161 gaactattta ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa
     8221 agagcgaaaa gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt
     8281 ttcggagctg tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg
     8341 atgcaaaaat taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt
     8401 gtgcttattg tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc
     8461 gatcaatggc ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat
     8521 gatgactatc tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt
     8581 gattgccagt tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa
     8641 tcaaaattcg gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa
     8701 aaaagccagt cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt
     8761 tttttcctca ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg
     8821 tcttctcata taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata
     8881 caaaagacaa atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa
     8941 aagataaatg atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt
     9001 ggtgctaatt gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc
     9061 ctgacaacgg ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc
     9121 gatttctcat ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat
     9181 aatggtaaaa catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga
     9241 aaagcggtat tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct
     9301 gcagaaactt tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga
     9361 agtacaatga tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt
     9421 gtttcgacac gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca
     9481 gttctgtcga ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat
     9541 gaagttactc aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga
     9601 ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca
     9661 tggcaattcg ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg
     9721 agcctgacgt tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg
     9781 ttgaatagtg taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta
     9841 tagcttctcg tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg
     9901 tttattcgta aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa
     9961 ggagctatcc aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta
    10021 catttaaaaa agctatcgat catggactaa tcatacatga tcgatagctt tttagataat
    10081 gtttgttacg ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg
    10141 agtagttcag aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg
    10201 tctgcatttt caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc
    10261 acagcttcca ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct
    10321 tcatctaaaa taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt
    10381 tgctgtcctt gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc
    10441 agtgttcgaa taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt
    10501 gtggcatcca ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta
    10561 ttttgtaaaa ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca
    10621 agaccgtcga attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact
    10681 aaagtcgttt ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact
    10741 gaaaaatcga cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg
    10801 aaagctattt ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca
    10861 tctgtttcat ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag
    10921 ttgatcacat aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa
    10981 gcttgtaaga agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca
    11041 cttatgatga aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata
    11101 aattgagcac gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct
    11161 gcatgagctt gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt
    11221 cgattattta aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc
    11281 gcaatcttag ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc
    11341 ttgctttcga tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa
    11401 aattgattga tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga
    11461 ctaatgatgt cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt
    11521 tttgtgattt cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt
    11581 tcctgaataa atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg
    11641 aacaacaatg cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt
    11701 tctgataaga gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg
    11761 ttcccgaata aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt
    11821 ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt
    11881 ctttctatat tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt
    11941 cttcatgagt accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt
    12001 gttggattgt actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat
    12061 gaagggctgc tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga
    12121 atacatagat ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc
    12181 cccctgaata attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt
    12241 ccactagttc ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat
    12301 attcatttcc catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag
    12361 ggacataact gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac
    12421 caagggttac ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac
    12481 tttttccaga tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat
    12541 caatatcttc tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag
    12601 cagctgcatt tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa
    12661 tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga
    12721 taatgaccaa taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga
    12781 ctacagcagt cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc
    12841 acaaaataga agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa
    12901 atattcggtt cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct
    12961 tttcttcata tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc
    13021 tcaatgtgat ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc
    13081 gtttcatcag tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg
    13141 ttagtaatag agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg
    13201 gtaagatcat ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca
    13261 ttcttgtcaa aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga
    13321 atttacggaa atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa
    13381 agtaactaga ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc
    13441 caattttttt tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta
    13501 ctaattttgg tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga
    13561 gagcgagtcc tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt
    13621 tttcctgata cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa
    13681 taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga
    13741 actatttgat ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc
    13801 aagtagacaa gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta
    13861 tgatatcgtt ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg
    13921 aaaaacattg acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga
    13981 actggtggtg atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt
    14041 gatgatcgat acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat
    14101 ctcaacggag tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt
    14161 tgggatgatg agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc
    14221 tagtgaagta catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta
    14281 tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat
    14341 cagagaaaga atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtttctatt
    14401 gacgaatacg gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa
    14461 aaaataggaa atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa
    14521 aaattttctg gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa
    14581 ttttagtttt ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata
    14641 tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt
    14701 ataattcatg gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt
    14761 gtatcagatg agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt
    14821 gttgcttaca ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac
    14881 atatttttta atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga
    14941 tagcattttt ttgcttataa aataattata agtatttaga gatttttctt ctaacattct
    15001 tcaaacaaga aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt
    15061 gtttttttat agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat
    15121 ttggtaatat tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt
    15181 tattttttgg aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa
    15241 aaagcagtat gactaatata aatactattg tcatttatgc aattttttat ggatagtaat
    15301 tttagagggg aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt
    15361 agcaaaaata gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga
    15421 atcaactaca acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga
    15481 aaaggtagtt attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac
    15541 cctttgcaat atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat
    15601 ttaatacaga ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct
    15661 aagaagatga ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc
    15721 aaataaagag caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg
    15781 gtggacagtt tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc
    15841 ctaaatgcgt ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc
    15901 gatgaatccc aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt
    15961 tttgggattg atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac
    16021 cttggtatca acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca
    16081 ttaacaaaat aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc
    16141 gtgaaatcat aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa
    16201 tcaatcaaca gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct
    16261 tgagttgaac cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt
    16321 agaaaattat cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga
    16381 acaaatttca taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg
    16441 ggaacataac ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg
    16501 aagttgatca agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac
    16561 cagacctgct aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga
    16621 agcaactcaa tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg
    16681 aattgaccgc aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac
    16741 cagaagaaag cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta
    16801 atataccaga agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta
    16861 taacgcagga atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct
    16921 ctgctgagcg ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag
    16981 gaggcgctgg cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat
    17041 cgattggctt aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac
    17101 tggcatttta ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat
    17161 taaatgaagc cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat
    17221 caggcggtac tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag
    17281 caaacacgga attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat
    17341 actatggctc tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc
    17401 aagacgtgac caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag
    17461 acggtgtagt ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta
    17521 catggagttc cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa
    17581 cgaatccagg tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag
    17641 tatcttttaa taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc
    17701 aatcgtattt tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa
    17761 gacaagagga tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga
    17821 gccgagacta ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa
    17881 gtgtggacaa aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct
    17941 cgatgttttc tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg
    18001 aatttgtgtc atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa
    18061 tagttcgttt cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta
    18121 aggtcaatgt agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga
    18181 atagtgacaa tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta
    18241 aagtaattaa aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat
    18301 caagcgattc acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg
    18361 aaccgcttcc tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt
    18421 tagatgcgac ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa
    18481 ccattgaaaa catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg
    18541 gtgaagtcct tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa
    18601 ccggt
//
LOCUS       DACEJZ010000062        18165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEJZ010000062 DACEJZ010000000
VERSION     DACEJZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.064"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..726)
                     /locus_tag="IW656_001904"
     CDS             complement(28..726)
                     /locus_tag="IW656_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0837611.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="IW656_001905"
     CDS             complement(719..2071)
                     /locus_tag="IW656_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0837612.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="IW656_001906"
     CDS             complement(2294..3694)
                     /locus_tag="IW656_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0837613.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="IW656_001907"
     CDS             3855..4592
                     /locus_tag="IW656_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0837614.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="IW656_001908"
     CDS             4717..5196
                     /locus_tag="IW656_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0837615.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="IW656_001909"
     CDS             5193..5960
                     /locus_tag="IW656_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837616.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="IW656_001910"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="IW656_001910"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0837617.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="IW656_001911"
     CDS             complement(6863..7465)
                     /locus_tag="IW656_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0837618.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="IW656_001912"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="IW656_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="IW656_001913"
     CDS             8146..8730
                     /locus_tag="IW656_001913"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0837619.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="IW656_001914"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="IW656_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0837620.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="IW656_001915"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="IW656_001915"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0837621.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="IW656_001916"
     CDS             10631..10810
                     /locus_tag="IW656_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837622.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="IW656_001917"
     CDS             10832..11857
                     /locus_tag="IW656_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0837623.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="IW656_001918"
     CDS             12032..13276
                     /locus_tag="IW656_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0837624.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="IW656_001919"
     CDS             13429..14376
                     /locus_tag="IW656_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0837625.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="IW656_001920"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="IW656_001920"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0837626.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="IW656_001921"
     CDS             complement(15278..15520)
                     /locus_tag="IW656_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837627.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="IW656_001922"
     tRNA            15641..15713
                     /locus_tag="IW656_001922"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="IW656_001923"
     CDS             complement(15832..16470)
                     /locus_tag="IW656_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0837628.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="IW656_001924"
     CDS             complement(16593..17225)
                     /locus_tag="IW656_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0837629.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="IW656_001925"
     CDS             17576..17875
                     /locus_tag="IW656_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0837630.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6238 a         3163 c         3577 g         5187 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatga
//
LOCUS       DACEJZ010000063        17639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEJZ010000063 DACEJZ010000000
VERSION     DACEJZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.066"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..172
                     /locus_tag="IW656_001926"
     CDS             <1..172
                     /locus_tag="IW656_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0837631.1"
                     /translation="ISRQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFA
                     EAYKHGRIIAYK"
     gene            185..571
                     /locus_tag="IW656_001927"
     CDS             185..571
                     /locus_tag="IW656_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837632.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1122..1346
                     /locus_tag="IW656_001928"
     CDS             1122..1346
                     /locus_tag="IW656_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837633.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1364..1792
                     /locus_tag="IW656_001929"
     CDS             1364..1792
                     /locus_tag="IW656_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837634.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1770..3188
                     /gene="terL"
                     /locus_tag="IW656_001930"
     CDS             1770..3188
                     /gene="terL"
                     /locus_tag="IW656_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ0837635.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3200..4735
                     /locus_tag="IW656_001931"
     CDS             3200..4735
                     /locus_tag="IW656_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0837636.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4815..5693
                     /locus_tag="IW656_001932"
     CDS             4815..5693
                     /locus_tag="IW656_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ0837637.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5695..6012
                     /locus_tag="IW656_001933"
     CDS             5695..6012
                     /locus_tag="IW656_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837638.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6061..6762
                     /locus_tag="IW656_001934"
     CDS             6061..6762
                     /locus_tag="IW656_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ0837639.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6777..7667
                     /locus_tag="IW656_001935"
     CDS             6777..7667
                     /locus_tag="IW656_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ0837640.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7690..7905
                     /locus_tag="IW656_001936"
     CDS             7690..7905
                     /locus_tag="IW656_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837641.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7917..8258
                     /locus_tag="IW656_001937"
     CDS             7917..8258
                     /locus_tag="IW656_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837642.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8258..8629
                     /locus_tag="IW656_001938"
     CDS             8258..8629
                     /locus_tag="IW656_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837643.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8622..9017
                     /locus_tag="IW656_001939"
     CDS             8622..9017
                     /locus_tag="IW656_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ0837644.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSIGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            9019..9396
                     /locus_tag="IW656_001940"
     CDS             9019..9396
                     /locus_tag="IW656_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837645.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9397..10005
                     /locus_tag="IW656_001941"
     CDS             9397..10005
                     /locus_tag="IW656_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0837646.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            10005..10346
                     /locus_tag="IW656_001942"
     CDS             10005..10346
                     /locus_tag="IW656_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837647.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10588..13377
                     /locus_tag="IW656_001943"
     CDS             10588..13377
                     /locus_tag="IW656_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ0837648.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            13367..14107
                     /locus_tag="IW656_001944"
     CDS             13367..14107
                     /locus_tag="IW656_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0837649.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            14104..16815
                     /locus_tag="IW656_001945"
     CDS             14104..16815
                     /locus_tag="IW656_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0837650.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            16828..>17639
                     /locus_tag="IW656_001946"
     CDS             16828..>17639
                     /locus_tag="IW656_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ0837651.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGT"
BASE COUNT         6275 a         2506 c         3704 g         5154 t
ORIGIN      
        1 gattagtcgt cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag
       61 gattagccgt gaagtgggtt attcagaaag aagcatacaa aggatgaagt cggaagctct
      121 aatagagttt gcagaagcgt ataaacacgg aagaataatt gcttataaat agggggacca
      181 tataatgtgg aaagattatg tatcgttgaa agagttgaaa aaagatcttg ttttcaaaag
      241 aattgtagaa tggtcagaga gtgaattaat tttagaagat ggaaccaaaa tggaagttgt
      301 atgtagcgaa tcagattgct gcgcatgggc cgaaggtgaa ttcaaaaatg taaagcttga
      361 tgcagtgatt acagatataa agatttttga taaaggtaac cgtctctata atggtgacgg
      421 acatacctct tatgctgaag tcgttgttta tcataatcgt aatgagatcg ctaaagcaga
      481 atgcacagcg aatgatggga atggcggcta ctattatagt gtttgtgctt taaaggtcaa
      541 agacaaactt tgtatagtga ctgacgcata aaaaaatggc ggttttttgg cggaatgatg
      601 gcggttttta gccatttatc agtgatatta tggtagtgtc gaaagattag tgataggtct
      661 gagacaaaat aataataaaa ggaacatcgt tttattattg tttcacaatt aagcttcgat
      721 agacagcaac ggaaatatta agaataagga tgtgaatttt aactccttct aaattgttct
      781 tattatctat catccgttgc tgtctattgt tacatacaga tcgcttaggc ggtcttttta
      841 ttttggccga aaacctacat tttcgatggc cgattattgg aggaggaata gcatgttcaa
      901 actatctgaa atcattaaga agtccgacgt tgagaaatta gagatgttaa aaaagaagtt
      961 gaagaaatag cagtctcttc gtgaggctgt tttattttgc tcacaaaaat agaccactat
     1021 cgggtaatag tggtcaggaa ttaaatgaaa aagatgttag ggttgttagc taagtataac
     1081 atcataacgc ttacaaaaca atgcaagaag gataaattat tatgagaact tactggtatg
     1141 tgtcattaaa caataaatat ccgaagccaa tgaaaggaca gcataggcgt gtagtcatgt
     1201 ctgttcaaat gaaggcgaag tattcgattg tagaaatgat cagagaggca acgccagtag
     1261 agattgatta ttgcaagcta gtctattgtg ggtgcggccg ttggaaagag gatcatgtac
     1321 aaaaaaatat cagcaagtac atatagagag gcggtgatta aggatggaaa aaagcgatac
     1381 gaaagcgaac agatacaaac ctactgctgc agagaaaaaa ctcttggaag tattgataaa
     1441 tccagataac ttaggcaaac cggtgcaaga gttgtgttca ctggcgaaaa taggtaggac
     1501 caaatattac gacgccatga gcaaagatgg ctttgttgat ctagttaacg aaacaacaat
     1561 ggatctgatc aaaggaaaag cttctgatgt ccttaatgcc acttacaagt atgcattgac
     1621 tgaaaaaggt catcaggata ggaaaatgat tctgactatt gctggaatct atgcagacaa
     1681 acaagaactc aaacattctg gcggcgtcga tatccgaaaa cagtatgaga aaatgtccga
     1741 tgaagaatta gaagagttgg tgaaacggta tgaaaaaatc aacgactctt aatcgtgttg
     1801 agtatgccgt tgttcaaaga gagctagcca ttcgaaaagc taggaaaagt ttttggctat
     1861 tctgccagct tctttatcca gagttttaca aagatgatcg gttgtatttg aaagatttat
     1921 gtcatacgct acaagatttt tacgaagata aaatcgacaa aaaaattttg gttattaaca
     1981 tgccgcctcg acatgggaag acttttacag cgcggctatt cgttttatgg atgtttggac
     2041 aaaatccaaa gacgaaaatt attaccggat cttataacca aatcctgtca tctttatttg
     2101 cgcaacaaac acgtgatgga atcatggttg aaaacgaaaa tgttaaggct gaatacttta
     2161 aagatatttt tccagaggtg caaatcaaat atggagatgc tgcaaagggt ttctggtctt
     2221 tagatggttc agaagaaaag aattatttag cgacttctcc tggtggaaca tccactggta
     2281 ttggcgcgaa ttatgtaatt gttgatgaca tcattaaaac cgcagaagaa gcctataacg
     2341 agcgtgtttt agattctcac tgggaatggt ataacaacac gttggcccaa cgaatggaac
     2401 ggccacgtaa acagatattg attatgacac gttggtcaag taatgattta gccggaaaaa
     2461 tgttgacgag acgaaaaaat aatgttcatc agatctgtta taaagcagta caagaagacg
     2521 gctcgatgtt gtgcaacgag attatgaccc acgatgagta tttagatgtg gtacaagaaa
     2581 tgaatgtaga tattgcagaa gccaactatc agcaagaacc aattgaccaa aaaggcaggc
     2641 tatatcagaa gtttcttact tatgatacgt tgcctgataa cattatcaaa atatggaact
     2701 acaccgacac cgccgacaag ggtgccgatt actttgcgtc tcctgttttt gctgagacgt
     2761 ctgatcatga tgcttatatt atcgatgtca tgtatacaaa agagccgatg gaaacaactg
     2821 aaatagccca cgctaatatg attattcgca atcgagttaa taaggtacgg attgaaggaa
     2881 acaatggcgg gcgtggattc agaagaaatt cagaaagaat tacaaaagag cgaggctatc
     2941 gaggtgcagt atttgaagac tttcatcaat cggataataa actgtctaga atactggcga
     3001 atagtgcttg ggtggaaaat catgtttact atccagatga ttgggcgaca cgttggccgg
     3061 acttttatga ggctatggtt agttatcaac gtgaaggtaa gaacgctcat gatgatgctc
     3121 cggatgcagt tacaggaatt gcagaaacga ttatagaagc agatgacttc gagtttattg
     3181 cttattagga ggtgggtctt tgttttattc tggaatcaca ttgaaaaaat acaaacgttt
     3241 aagaacgaaa tattcaacgc aaatcgaaga aggattattc gatcctaacg cttttatgga
     3301 agatatgaaa ccctttttcg atgatcgaga aagaaagtat cttgcttata ctagcgagaa
     3361 aaacgagatc gacagtcgtg agaaaccaaa aacaaaaatc attaaggtca ataacaagat
     3421 tcatgcggga atgtattcag tggtcgttga tcaagcagtt aaccatttca caggaatacc
     3481 aattaaatgg gattatgatg tttcggaaca gaaaaggaca ttcattcaac gaatgagaga
     3541 caaattactg ggtaatgata tcagcgcccc ttctacccca gaagaatttg aaacactcac
     3601 gagacaatta agtactatgc ggtttggaat gttagacccc gatactgcaa catttcaagg
     3661 agcgacaggg cttgctttca gattactgga gcctgttgag acaaatgatg gttgggaact
     3721 aagagcaagc aacattgagc cgtggaaagc tgaaaagtac gaaaacggcg cagtgtacat
     3781 taaagagaaa tacgatccat atcaaaagaa gttttatgaa gagatgaagg ttgtcacgcc
     3841 gaaatcaata tttacttatg ctcgatatac tgattaccct ggcacggtac aaggagcatt
     3901 taaaacgctg ggtaatgttc ctaatccttt aggcgttatc aatcttacgg agtttaaaaa
     3961 caatacaaac cgttactgtg actttgaagt tgcagaggag attagtgatg ctattgatcg
     4021 tgttttatct gatcagcaga acgagattga acaattcaag ttagcttata tgctgatcac
     4081 tggggcagat ttgtccgagg ataaagccaa gaagatgatg gagcaactag ggatcatcaa
     4141 tctgaaagac cctaatgcga aagccggcta cgtgacaaag gatctacaaa aggatttcaa
     4201 cgagtatcat tttgatcttc ttaaaaagca attctacacg gtgtgtaaag caattgattt
     4261 caatgacgaa gtctttaagt cgaatagttc tggggaagcc cgaaagtggc aaatcattag
     4321 tttagaagct aagacaaata ccaaagaaca gtactttcga gaaggattga aagagtgtgc
     4381 cgaggttatg gctgcttatt tggattttgc cgagaaagtt acaattgatc cggaaaagat
     4441 cattttcacc ttctctcgtt cgcttccaac agatttaagc tacctagctg aggcattgcc
     4501 taagttggca ccatacgttt ctaagaggac gattcaaagt cagatcccgt tcgtcacaga
     4561 tgttgattat gaaaacgaaa tgattgacgt cgaaagcaga caatcttacc cagacggcga
     4621 gtatggaggt ggcatcgttg agtcaaatag aaacgaagta ctggaccaaa cgtcgagaac
     4681 tggaagattt agcaagactc aaaatggaaa atcaaacgct aaagaaacta agtagtgtct
     4741 ttcctgatgc gttgaaagaa attcaagcgg agttattatc ccaagctgat ttacacgata
     4801 ttacctatgc tgagatgatg gaattttata gtacaagtga tcagaaaaaa tatcgtgaat
     4861 acgttgaaaa gaactacaaa tcactgaaga tgtatgatgc aaaatacaaa gagttcatcg
     4921 aagaatactt tccttctttc gactacgcca aagtgaatcg gctgcttcaa atgaggtcag
     4981 atatttttcg aattcttgca gagagtgcaa tagatgcaga tgtgaataaa tatttttcgg
     5041 atcggttaga agatatcttg caacgttcgt atgtatcaaa cgcaaacgta tttgcccaga
     5101 ttttgaacgt agatttgccg aattacttat caaaggaaga acttgaacgt tacctgaact
     5161 atccttggtc aggtaagaca ttctcgcgtc gtttatgggg caatatttca tctctagaac
     5221 aaaagctatc taacgccatt acaactagtg tagcaagtgg cgaaggagtt ctcgtggcat
     5281 tacgaacaat gcgagaagac ccagaaatat gcgatatgtt caaaagggag gaatcgaagt
     5341 acaacaaggc gatcgaaaat cttgtgagaa cagaatacgc taaattcgct caagatggta
     5401 ttgagaaatc ttatattgaa actggcgtag acgaatacaa tgtcttaact gcaaaagatg
     5461 aaaaggtatg ttcaatatgc ggtggcaaag caaagaacaa cccatacaag ttgagtgaag
     5521 cgatcattgg tgagaaccgt gcgccttttc atggccgatg cagatgtaca gatgttccta
     5581 atatgccgaa attaggaaaa gatattgacg aagagtatga gcgtttattt ggcgatttat
     5641 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
     5701 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
     5761 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
     5821 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
     5881 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
     5941 gttgattggt atgaaaatat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
     6001 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
     6061 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
     6121 tcgtggggcg aaaggagaat gattatgaaa cacaaatcat taatgccaat gaatttgcaa
     6181 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
     6241 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
     6301 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
     6361 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
     6421 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
     6481 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
     6541 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtt
     6601 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
     6661 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
     6721 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
     6781 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
     6841 aagtaccaaa tacacctttt ttaagctatt tacttggtgc tggaaaaaca gagccagcaa
     6901 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
     6961 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
     7021 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
     7081 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
     7141 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
     7201 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
     7261 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
     7321 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
     7381 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
     7441 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
     7501 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
     7561 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
     7621 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
     7681 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
     7741 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
     7801 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
     7861 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
     7921 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
     7981 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
     8041 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
     8101 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
     8161 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
     8221 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
     8281 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
     8341 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
     8401 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
     8461 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
     8521 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
     8581 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
     8641 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
     8701 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
     8761 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
     8821 caatcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag
     8881 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
     8941 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
     9001 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
     9061 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
     9121 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
     9181 gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat
     9241 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
     9301 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
     9361 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
     9421 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttc
     9481 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
     9541 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
     9601 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
     9661 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
     9721 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
     9781 ggacagtttg ctctagattc agatgaaacg tcaacgattg aaggaagtaa agtaagtttt
     9841 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
     9901 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
     9961 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    10021 caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat
    10081 ctgctgaatt tttaccagga acagttgtag aagatgctgc aggagttatg gaagaaatgc
    10141 aaacagcgac tgataaacaa tcagttaaaa aagctttaag tcgtgcttat tcatttattg
    10201 cagatacttt atttgaggga caatttactg gtgaagatta ttgcaaagga attgatgctc
    10261 gtgagattgc ctcattgaca ggaaaattat tgaagtctgt tactgcaggt tttgatgaaa
    10321 cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat ttaagtattc
    10381 gattacttac cgagaattag atataaagac gcaattactc gaagcaggtt ggacgttacc
    10441 agaaattgaa cgtactgact tggatgagtt aatgcgtctt tttgctttta gagatgcggt
    10501 aaaagagcat gaagatgttg agtactatga taatttcact caattttagg aggtgatact
    10561 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    10621 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    10681 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    10741 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    10801 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    10861 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    10921 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    10981 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    11041 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    11101 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    11161 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt atctcaaggt
    11221 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    11281 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    11341 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    11401 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    11461 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    11521 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    11581 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    11641 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    11701 atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca
    11761 atagctgttt tcggttactt tatggcaacc aatgaagagt ttagaaacaa ggttatatct
    11821 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    11881 attgatactt ggaattctgc taaagaaatg gcatcaaatg ctgttgagag tgtcaaggat
    11941 gcttggtctg gaataaagga atggttttca aatacttggc aaggcattaa aaatggagca
    12001 acaggtttat ttgataaaac agtagaaaca tctatgaatg cggttgatag tgtaaaaaac
    12061 gcatggtcaa atacaaagca atggttttct gatatttggc agagcataaa agattcagca
    12121 actgaaaaat ggaatgaaat taaaggttct atcatggaag ttgctggtcc attgattaca
    12181 ggtttaaaga acgcattctt gcatgtcact ttttatttag agaccttatg gaataatcta
    12241 gttgagattg gtaaaaatgt ttttgagatt ttaaaaaatg ttatacttac gccagttttg
    12301 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaacaacat gattggtgta
    12361 tggaataata ttaaagaaag tgctctaaat atttgggagt ctataaaaaa tatttttgtt
    12421 agttacatta caaatattta ttttgctgcg cttaatattt ggacagggtt caaacttact
    12481 ttgataaaca tttggaatga agtagtaagt caagctaaat caatttggat taatgtaaaa
    12541 tactttttta ttaatctttg gattgacatt aagtattttg ctattcaaaa atggattgaa
    12601 ttaaaattcg ggattattca aacttggatt gatttaaaat ataatgctat tactacttgg
    12661 aacaatatta aacagttctt caaagatact tggcagaaca ttaaagatat agcatacaat
    12721 acatggattt ctataaagaa ttccatgatc aatacttgga ataacatcaa ggaatctttc
    12781 tggaatattg ttactggaat tgttaactcc gctgaaaatg catggactaa tctaaaaaat
    12841 ggtgtttcaa aagcaattaa tcgggtgaaa gaaatctttg attcattaag ggaaatcaat
    12901 ttattcgaaa tcggtaagaa tatcattgat ggacttatca atggtgtagt agaaaaatgg
    12961 aatgcattga aaaagactat taaaggaata gctggaagta tcaaagattc tattaagggt
    13021 gcattaggta ttcattctcc atctagatgg atgagggata tggtaggtaa aaatattgtt
    13081 caaggtatta tcgttggtat tgataaagaa aaaagtaaat tggatcagac aatgactgat
    13141 ttagtgaaaa ctccgtcggt tcaaccagtt attacagggt ctaatagtca accagtggta
    13201 caagctaaac aaaatacatc ttcaaatgca gtgaatgaaa ttcacttgca tttaaatgtt
    13261 tatggagatc tacctgattc gatgattaaa caaatcgcca agaagatgaa aacagaattg
    13321 acgagacaaa tgaaacgaga tgctgatgca gtaggaggga cattatatgc aacttaaaag
    13381 aggacaattt tttatcaatc aacattattc ttctgagttt aacgtatata ttcaaaatag
    13441 gcctgcctct gtttcagcta gtcgtgtaat tgaattgaga gaacgtgaag gcaatgactc
    13501 aattattatc gataaagcct attacaaaaa cgttactaga aaaattgaat gttattacaa
    13561 agcaccatcg attgatttag tgcaggaatg ggaagatcga attactgaat ggttagatat
    13621 gaactcttac agtgatttta ttctttatta cgatcaacaa tatatctatc aagctatagt
    13681 aaccgaagcg ccagaattta aaggaacaag aaaaacaggg aacatagttc catttgaatt
    13741 tacagttagt cttcgaccat tcaaagaaaa ttatagtggt cgttttgcta ttcaacagat
    13801 aaaagctttc gagttattta atccagagaa gtatgcttca aaaccgctta ttaaattgag
    13861 tggttctgga gatgcttctt tttatattaa taatgataaa tatgatttga aactattaga
    13921 tagagaatta tatatagatt ctaaactaga agagtcttat cgaaagctag atgacaattt
    13981 agaacatcaa gatcatgtca ctttattttt agattttcca tttctatacc ctggaaaaaa
    14041 tgaaatcaaa tggacgaata acatacattc gtttgagata ataccaaggt ggtggagaaa
    14101 agtatgatac caagaatata tagtcctacc gagacagatt tttctacgaa tggtttaggg
    14161 attttgaagg acaccacaaa atgtgagata tatgaagtag caaatggaaa atatgaatta
    14221 gagttggagt atccattagg tactcgattt gatgaatatt ttgaaaatga ctatcaaata
    14281 aaagcaaagt caaatgatca agaagagtat catatctttt ttattgatga taaagatatt
    14341 gatacctttt tagataccgt aactatttat gcccagagtc gtacaaatcg gcttggaaga
    14401 cgagcggtca cttttgctgg agtcgactct aaaactggtc gagaagcaat ggcaattatt
    14461 gaaaacaata tggataaaaa atcagatata cgcttatatt ctgatattac aactgtatca
    14521 agtacaactt ttgaagcaag aaatgtttta aattgtattg ctggtgaaca aggatcttta
    14581 cttcagtatt ggggcggaga aattaaacgt gagcctttta agctatcttt attaaagcga
    14641 agaggtcgtg ataatattgg cacaatccga tatggaaaag atttatctgg gttaaaagtt
    14701 aagttagatt ggacaggcgt gaaaacaaga attattccgt atgctgatcc tcaaagtgat
    14761 gcaggtacaa ctagccgaat ttatggctcg ccagtagata gcgaatatat taataattat
    14821 cctgatgtat atacagaaca cgttcaattc acagaagaac agggagttaa agacgttaat
    14881 agcttaaata aaatagctaa gaattatttc aaaacaatta atccaggctg tgataaaccg
    14941 aagatttcta ttaccgttga gttcgataaa ttaacggaca ccgaagaagg aaaagaattc
    15001 gctaagataa gaaattatgg gttatttgat acgtttaaaa tatatcatcg aaaatataaa
    15061 ctatattttg aatcgaaagt tagcggtgtt caatatgatt cattatctga aaaggttttg
    15121 aaattggaag ctggggatgc tcaagttgct ttttatcaac aacaagctgt tactattcaa
    15181 gataaattaa aagattatgc tacgaataat tatatgagta gttttaatga ttatgtgtcc
    15241 tctatgattg caggtcaagg aaatgcaggc ggttatgtag ttttatggcc aaaagaaaaa
    15301 ccttcaaata tttttataat ggatagccct gatttaaaca aggcaaaaga agtattaaga
    15361 atgaacaaaa atggaattgc cttttctaaa aatggttgga atggtccttt taattccgct
    15421 tggacattgg atagtatatt caatgctaat tttattcaaa caggattaat aaaggcagat
    15481 atctttcaaa actcttttaa taaaactgga gatgtattga aattagttaa tggactactt
    15541 caaatttgga ataacaaaaa gaaaataatg gagttaacca aaaaaggaat ggagttttgg
    15601 aattctaata gttcaattgg aacgattgga acaactgatt ctgctggtaa tccttttcct
    15661 ggagcttcta ctcccacacc tattgaagat aattctttag ttattcgtac aaatggagac
    15721 ggcaaatata ttttgatttc tcctaaagtt ggtaaaggat tagttttatt aggaaatggt
    15781 aaagcaattt attttggaga cttagatgta caaggcaaac tcacagttaa cggaaaagaa
    15841 atcacaggga acaacagtgg aggaagtgat cctggaacta tccctcctca attgacgacg
    15901 gaagctgaaa aaagagcatg gaaaatttgg acaatgctga aagctcgtgg ctattctgaa
    15961 tatgcagctg caggtattct aggaaatatt cagggggagg ttggagcaag tatgaaccct
    16021 gatacagaac aacttggtgg tccagcttat gggattgttc aatgggatgg ttctgcttat
    16081 ccgttagtcg gatcaccaac ttggaacgga cgagagtatg ttcagcgttt gatgaacacc
    16141 gcagggattc aagaagatta tcgaagtatt gaagcccaag taaaattatt agattggtgt
    16201 atgttcaacg gtcaatggct cggaaaagta aatccaacca cagtatcagg atttaaatcg
    16261 atcaatgatg ccaaaagtgc agcgtatgct tttgaaatga acttcgaacg cccagcttct
    16321 gcacatccag aacgccaaaa ttatgcccaa tcttggtata acaaattaca tggattaact
    16381 agtccagaac ctggagggaa tttcatttgt ccaattcaaa aaccagtgac agttacttca
    16441 gaatgtggat ggagaactag tccaataaat ggcggccaag aatttcataa tggaattgat
    16501 cttgtaaatg gaaatcctaa tacacctgtt tttgcagcat tagatgggga agttgttcag
    16561 gctggtgcta attattatga ctggtatggt aattacgtgg ttattaaaca taataatggg
    16621 aagtggacag gttatgctca tttgtctcgt attgatgttt ctgttggaca aaaagtccag
    16681 aaaggtgctc aaataggctt gatgggaaca actggtccat ctacaggaga acatctacat
    16741 tttcaaatta tgaaaaatta ttggccacag ccagttgttg attttgagaa tccaagaaat
    16801 tatatccaat tttaaggtgg tgattctatg agtaaatgga atgtcgtttt aagtacaaca
    16861 gaaccatata attatgtggg gatgattcaa gttcgacaag gcaataagaa tacagaggtt
    16921 atggaagcga ctatagttga aaatggtctt ccctacgatt tatcagaatg taaggtatat
    16981 tttgaatcag ttgtaggtgg gaaatatcca gtccaattag aaacaaaaat tgtggatgct
    17041 aaaaaaggga aaattaacta tatttttgat aaatattcca tgcagtgttt gcatcgacaa
    17101 acagccaatt tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc
    17161 tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca
    17221 attgaagatt taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg
    17281 gactggatga atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt
    17341 ttccaagatt ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag
    17401 gatattttga agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt
    17461 gttgatattc aaggagttcg tcatgcgtct atttcagagc gcttgctggc agatatggat
    17521 tatctatatc agaaattacg agcaacactt ttcacgattg aatacggtga gattgaagtg
    17581 actgatattt tgcaggatga tctcttttca gataatcacg aagtcgaaaa agttggaac
//
LOCUS       DACEJZ010000064        17428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEJZ010000064 DACEJZ010000000
VERSION     DACEJZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.067"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            90..308
                     /locus_tag="IW656_001947"
     CDS             90..308
                     /locus_tag="IW656_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837652.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(597..1109)
                     /locus_tag="IW656_001948"
     CDS             complement(597..1109)
                     /locus_tag="IW656_001948"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0837653.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IW656_001949"
     CDS             complement(1123..3420)
                     /gene="recJ"
                     /locus_tag="IW656_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0837654.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3562..3996)
                     /locus_tag="IW656_001950"
     CDS             complement(3562..3996)
                     /locus_tag="IW656_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0837655.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4006..4794)
                     /locus_tag="IW656_001951"
     CDS             complement(4006..4794)
                     /locus_tag="IW656_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0837656.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4791..5732)
                     /locus_tag="IW656_001952"
     CDS             complement(4791..5732)
                     /locus_tag="IW656_001952"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0837657.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5909..6496)
                     /locus_tag="IW656_001953"
     CDS             complement(5909..6496)
                     /locus_tag="IW656_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0837658.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IW656_001954"
     CDS             complement(6717..8030)
                     /gene="obgE"
                     /locus_tag="IW656_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0837659.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8318..9328
                     /gene="gap"
                     /locus_tag="IW656_001955"
     CDS             8318..9328
                     /gene="gap"
                     /locus_tag="IW656_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0837660.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9413..10348)
                     /locus_tag="IW656_001956"
     CDS             complement(9413..10348)
                     /locus_tag="IW656_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0837661.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10534..11493
                     /locus_tag="IW656_001957"
     CDS             10534..11493
                     /locus_tag="IW656_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0837662.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11579..12673)
                     /locus_tag="IW656_001958"
     CDS             complement(11579..12673)
                     /locus_tag="IW656_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0837663.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12862..13623
                     /locus_tag="IW656_001959"
     CDS             12862..13623
                     /locus_tag="IW656_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0837664.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13738..14178)
                     /gene="fabZ"
                     /locus_tag="IW656_001960"
     CDS             complement(13738..14178)
                     /gene="fabZ"
                     /locus_tag="IW656_001960"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0837665.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            14398..15153
                     /gene="fabI"
                     /locus_tag="IW656_001961"
     CDS             14398..15153
                     /gene="fabI"
                     /locus_tag="IW656_001961"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0837666.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(15263..16276)
                     /locus_tag="IW656_001962"
     CDS             complement(15263..16276)
                     /locus_tag="IW656_001962"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0837667.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            16441..17361
                     /locus_tag="IW656_001963"
     CDS             16441..17361
                     /locus_tag="IW656_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0837668.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         5081 a         3728 c         3059 g         5560 t
ORIGIN      
        1 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
       61 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
      121 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
      181 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
      241 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
      301 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
      361 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
      421 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
      481 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
      541 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
      601 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
      661 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
      721 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
      781 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
      841 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
      901 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
      961 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     1021 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     1081 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     1141 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     1201 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     1261 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     1321 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     1381 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     1441 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     1501 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     1561 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     1621 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     1681 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     1741 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     1801 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     1861 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     1921 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     1981 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     2041 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     2101 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     2161 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     2221 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     2281 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     2341 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     2401 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     2461 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     2521 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     2581 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     2641 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     2701 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     2761 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     2821 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     2881 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     2941 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     3001 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     3061 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     3121 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     3181 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     3241 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     3301 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     3361 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     3421 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     3481 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     3541 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     3601 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     3661 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     3721 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     3781 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     3841 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     3901 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     3961 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     4021 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     4081 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     4141 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     4201 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     4261 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     4321 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     4381 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     4441 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     4501 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     4561 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     4621 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     4681 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     4741 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     4801 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     4861 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     4921 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     4981 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     5041 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     5101 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     5161 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     5221 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     5281 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     5341 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     5401 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     5461 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     5521 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     5581 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     5641 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     5701 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     5761 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     5821 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     5881 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     5941 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     6001 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     6061 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     6121 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     6181 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     6241 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     6301 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     6361 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     6421 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     6481 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
     6541 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
     6601 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
     6661 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
     6721 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
     6781 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
     6841 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
     6901 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
     6961 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
     7021 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
     7081 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
     7141 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
     7201 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
     7261 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
     7321 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
     7381 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
     7441 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
     7501 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
     7561 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
     7621 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
     7681 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
     7741 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
     7801 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
     7861 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
     7921 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
     7981 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
     8041 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
     8101 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
     8161 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
     8221 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
     8281 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
     8341 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
     8401 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
     8461 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
     8521 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
     8581 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
     8641 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
     8701 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
     8761 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
     8821 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
     8881 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
     8941 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
     9001 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
     9061 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
     9121 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
     9181 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
     9241 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
     9301 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
     9361 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
     9421 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
     9481 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
     9541 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
     9601 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
     9661 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
     9721 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
     9781 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
     9841 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
     9901 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
     9961 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    10021 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    10081 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    10141 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    10201 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    10261 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    10321 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    10381 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc
    10441 gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt
    10501 ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac
    10561 gaactgacaa tgatagaatc atattatctt caacataata agccggttga aatcgctaac
    10621 cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa gcaaggcaag
    10681 actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc
    10741 attcaattac ctgctcatga agtagattac attaaagaga aagtcactct tggttggacg
    10801 cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat gcggtatgag aacactttat
    10861 cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg taaaagaaaa
    10921 cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat ccatgatcga
    10981 cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga tacgatcgtt
    11041 ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa agtcattatc
    11101 acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa tcaatggttt
    11161 tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa agaattttct
    11221 aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga ccctggaaca
    11281 ccttctcaac gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg
    11341 aaatcaatgg attttagaaa agtgaatcag acatttattt ccagtgtcag caatcaacgt
    11401 aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat
    11461 gtacaagaag cattttattc tagcttaatt tgacaaatca taacctataa aaaaacaaaa
    11521 taaaaaagca gaaataactg gctatttcta gcggattatt tctgcaataa ctaattatct
    11581 attttttatt ttcaattaat ttaaagcttg accatggttt caaaaagtca aggaggaata
    11641 attcgtcatc ggagattctt cctacaacat tgacacgtcc gtcgtttttc atatccttca
    11701 aggcgatttg tgtttctcct ttgtattgac cgtacccttc attgtcaatc agtacatccc
    11761 catgatgcat atctcgcgta ttatgaggcg ggaaatcttt atctttgtag tatacacgag
    11821 tcatcgttga acgaaggata tattctgagc gatcccctcg ataactatga aggttatcaa
    11881 acaagcagat tcgttcgttt tctgtaatac cttcttccgt atctactttc aatgttggat
    11941 aatccgcgtt aaatgcttct gccatttctt tcaattcttc ttcacttgcg tacgcatttc
    12001 caatagaaat atcatcaatc aatcctgtaa ggattaaatg cttcacttgt gtcgcaattt
    12061 caagatctcg gtggtcttcc agcgtgcaca agccatcttg cgtaggccaa gggccaaact
    12121 cagcagattg cgaattgacg aatgccatcg tattcaaatt atattttctg aatttttctg
    12181 aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg atgaggataa aaattatgag
    12241 aacctaataa attatccgtg tttggcgaat aactcatgat gttgtcaacg taattcgtgc
    12301 ccgaactcat attgatctcg attttgatac caaaaggatt gcgcgtcatt tttgcttctt
    12361 ctgctcctgt gaatccgata tctaagcgga ccccgtaggc acccatctta tggaaaaaag
    12421 ataagtcatc gtaagaaatc tccaactgtt caaataacgc tggattgata tcaaccatta
    12481 cttccatgcc taggctattc gcgtaatcaa ctacttcttt aaattctgaa agaacttttt
    12541 ctttatcatc attgatctgc agcaaactgg taaaaacccg tttataccca tacttatgag
    12601 ctaaatccaa gtacgctttg tccttttcaa atgttgaccg ttctggataa atcgagattc
    12661 ctaattttcc catattacca tttcctcctg catcttttcg tttttgtatc cacttacatt
    12721 atagcaaaca caaagtaaat ggtctacacc ttttcttttt cttttaaaat aaatgataaa
    12781 ttccaataat ttaagtaggt attttccctc tattttgtta tcattaaaga taacaaaata
    12841 gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca
    12901 gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct
    12961 acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg
    13021 aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa
    13081 atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg
    13141 gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc
    13201 gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa
    13261 agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt
    13321 gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt
    13381 caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc
    13441 gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa
    13501 gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac
    13561 aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa
    13621 taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt
    13681 ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca
    13741 agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat catctacata
    13801 tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt
    13861 cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata
    13921 agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag
    13981 tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa
    14041 atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc
    14101 tacataatca atataacaaa taggataacg attaggaatc aattccatta cttcagtagc
    14161 agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt
    14221 tgactatcaa agtatcatat tcctaaatat ttagcaatct ttttttaatt taagaaaaaa
    14281 tagtttgaca atcaaaacat tttgactaat aattaagaaa tcaagaagtt attttagcta
    14341 ttattctcta tttttttacg actatcaaat ttttatcaga aacaaaggag agtcattatg
    14401 tcatttctag aaggaaaaaa aatcgtcatt atgggcgtcg ctaacaaaaa aagcattgct
    14461 tggggttgtg ctgaagcaat gttgaaacaa ggggcagaaa ttatctatac ttaccaaaat
    14521 gaacgaatga aaaaatcatt agtcaaattg gtcggcgaag aagcattcac tgtagaatgt
    14581 gatgtttcta gcgacgaaag catcgaaaaa gcaatgaatg ccatcggtga atatgctggg
    14641 gagatccacg gccttgtcca tgcagttgct tacgccaaca aagaagaatt gtccggaaat
    14701 gtgtctgata tttccagaga cggctatcaa ttagctcaag atatcagcag ctactcttta
    14761 attgctgtat caaaatacgc acaaccgttt cttgcaccaa acagcgggat cgttagcatg
    14821 acttatctag gttcagaacg cgcaatcccg aattataaca tgatgggtat tgcgaaagct
    14881 tctcttgaag ctgctatccg ttatttggca gctgagttct cgcctaaagg aattcgggtc
    14941 aatggaatct cagctggggc aatcaaaaca ttagctgtca caggtgttaa agactatcaa
    15001 aaactgatcc agttatctga atctcgtacg cctgatcagc aaggtgtgac gatcgaagaa
    15061 gtcggtaatg cttgtgcttt cttaatcagc ccactttcca gcggaatcat cggtgatatt
    15121 atctacgttg acaaaggtgt ccatctttca taaacttttg ttgcattctt taaagtaaaa
    15181 catataaaaa tcaggaaaaa actttttaaa gtctcttcct gattttttct ttttacgttt
    15241 ttttcaactc attactttga aattattcca cgacttcaat ccagccttct ggtgcttcta
    15301 tgtctccaaa ttgaatacct gtcaattcat catacaaccg ttttgttact ggtcctactt
    15361 ccgtttcact ataaaataca tgaagattgt tattgtgata aatcccaccg attggagaaa
    15421 taacagccgc tgtaccacat gcaccagctt ctgcaaattg atctagatca tcaatgaaaa
    15481 tatctccttc aattgcttgc agtcctagtc gttcttcagc taactgcaac aaggaatatt
    15541 tcgtgatact tggtaagata gagggcgatt gcggagtgat gaattttccg tcttttgtaa
    15601 ttccaaagaa atttgctgca cctacttctt cgattttcgt atgtgtggct ggatcaagat
    15661 aaacacaatc tgagtaatcc agttcttttg cttctgttcc tggaagtaaa ctcgctgcgt
    15721 aatttccgcc gacttttgct gcaccagtcc ccttaggagc agcacgatcg taatcagata
    15781 caataaaatt cgttggtttt agtccacctt tgaaatacgc tcctactggc gtacagaaga
    15841 cgacaaataa atattctgga gctggatgga caccaatgtt ttcaccgacc ccaatcagta
    15901 aagggcgcaa gtataatgta gcgcctgttc catagggcgg cacaaaatct tcatttgctt
    15961 ttaccacttg cttgacagca tcaacgaaca tcttttcagg gaccattggc atcagcaatc
    16021 gtctagcact ttgattcatc cgtcgactat tttgttcagg tcgaaagaga ttgaccttgc
    16081 cgtcttttcg acgataagct tttagacctt caaaacattg ctgaccataa tgaagcgctg
    16141 gagagccttc atgaatcgtc aggtaattat cttctgtcaa ttctccttct tcccattgtc
    16201 cgtctttcca tttggcaata aaacgccaag gagttttgat ataagaaaaa cctagattgt
    16261 tccaatcgat ttgcatatac ttaccacctt cgttattttc tccacatttt atcattatcc
    16321 atattatttg tcattttatt ttcagctttt ttccttcatt atagttattt ttgctatttc
    16381 cttttttgaa aacttcttgg tacaatacag aaagaatcaa attttagaaa agaggatttt
    16441 atgttttcat tattagcaac tgccggtacg acagactctt ctggcgagac attgacagat
    16501 caagctgtca gcaaagtaaa tgcgtttcag cggttttggg acgggattga ttgggacgcc
    16561 attttggctg cctttatcca aaaaagttta tatctgattt ttttgatcgt ccttttttgg
    16621 attcttcacc gtgtgggaaa atatctgatt gataagtctt ttaatcagta ttctaaaaaa
    16681 gccattctaa acgagagccg cttaaagact ttacatagtc ttttaaatac tatttttcat
    16741 tatacccttg gtttcttttt tatctatgca attttatctg ctatcggtgt gccgatcgga
    16801 tcacttttgg ctggtgccgg aattgcaggt gtggcaatcg gtcttggtgc ccaagggttc
    16861 atgagtgatg ttattaccgg attttttatt atcatggaac aacagatgga tgttggggac
    16921 tacatcaagc tagcaaactt aacgattgaa ggaaccgtcg tatctgttgg tatccgtacc
    16981 cttcagctga aagcaacgga tggtactgtc catttcatcc cgaatcgaaa catcaccacg
    17041 atcagcaatc tctctcgtgc aaatatgcaa gtacttttag atattcggat tgttcctgaa
    17101 gaaggatatg acaaaatcta tgagatcatt gatcgagtaa atcgtacatt agctgaaaaa
    17161 taccaagaag agctccaaac tgaaccttct atctttggat tagtggatct tggaaacagc
    17221 aattttgcga ttcgtacggt ctgctacgca ttgaacggaa aacaatatgc cttaaaagaa
    17281 gagttcttga gctcctatgt caaagaattg acagcttctg gtttcacgat tccgaacagt
    17341 ccaattgctg tcggaaaata atttggacaa acgatgcaac gaatctcact aaaaagagcg
    17401 tgggacaaaa gtaaagaaca cttttgtc
//
LOCUS       DACEJZ010000065        17006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEJZ010000065 DACEJZ010000000
VERSION     DACEJZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.068"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..125)
                     /locus_tag="IW656_001964"
     CDS             complement(<1..125)
                     /locus_tag="IW656_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0837669.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="IW656_001965"
     CDS             complement(229..540)
                     /locus_tag="IW656_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0837670.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="IW656_001966"
     CDS             complement(542..739)
                     /locus_tag="IW656_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0837671.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="IW656_001967"
     CDS             complement(1133..2860)
                     /locus_tag="IW656_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0837672.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="IW656_001968"
     CDS             complement(2853..4046)
                     /locus_tag="IW656_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0837673.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IW656_001969"
     CDS             complement(4233..4877)
                     /locus_tag="IW656_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0837674.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IW656_001970"
     CDS             complement(4971..5105)
                     /locus_tag="IW656_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0837675.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IW656_001971"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IW656_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0837676.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IW656_001972"
     CDS             complement(7236..7709)
                     /locus_tag="IW656_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0837677.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IW656_001973"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IW656_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0837678.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IW656_001974"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IW656_001974"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0837679.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IW656_001975"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IW656_001975"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0837680.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IW656_001976"
     CDS             complement(11756..12895)
                     /locus_tag="IW656_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0837681.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IW656_001977"
     CDS             complement(13204..13917)
                     /locus_tag="IW656_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0837682.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IW656_001978"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IW656_001978"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0837683.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="IW656_001979"
     CDS             15106..16638
                     /locus_tag="IW656_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0837684.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="IW656_001980"
     CDS             complement(16829..>17006)
                     /locus_tag="IW656_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0837685.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DACEJZ010000066        16527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEJZ010000066 DACEJZ010000000
VERSION     DACEJZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.069"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(141..416)
                     /locus_tag="IW656_001981"
     CDS             complement(141..416)
                     /locus_tag="IW656_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0837686.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /locus_tag="IW656_001982"
     CDS             complement(659..1969)
                     /locus_tag="IW656_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0837687.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW656_001983"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW656_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0837688.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="IW656_001984"
     CDS             complement(3515..4195)
                     /locus_tag="IW656_001984"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0837689.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="IW656_001985"
     CDS             complement(4244..4879)
                     /locus_tag="IW656_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0837690.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="IW656_001986"
     CDS             complement(4945..6348)
                     /locus_tag="IW656_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0837691.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="IW656_001987"
     CDS             complement(6335..7366)
                     /locus_tag="IW656_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837692.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="IW656_001988"
     CDS             7397..7618
                     /locus_tag="IW656_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0837693.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="IW656_001989"
     CDS             complement(7783..8520)
                     /locus_tag="IW656_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0837694.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="IW656_001990"
     CDS             complement(8673..10013)
                     /locus_tag="IW656_001990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0837695.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="IW656_001991"
     CDS             complement(10250..11773)
                     /locus_tag="IW656_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0837696.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="IW656_001992"
     CDS             complement(12151..12762)
                     /locus_tag="IW656_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0837697.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="IW656_001993"
     CDS             complement(13092..13808)
                     /locus_tag="IW656_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0837698.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IW656_001994"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IW656_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0837699.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="IW656_001995"
     CDS             complement(14367..15164)
                     /locus_tag="IW656_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0837700.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="IW656_001996"
     CDS             complement(15134..15505)
                     /locus_tag="IW656_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0837701.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IW656_001997"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IW656_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0837702.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5507 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DACEJZ010000067        16488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEJZ010000067 DACEJZ010000000
VERSION     DACEJZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.070"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..1065
                     /locus_tag="IW656_001998"
     CDS             127..1065
                     /locus_tag="IW656_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0837703.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1080..1910
                     /locus_tag="IW656_001999"
     CDS             1080..1910
                     /locus_tag="IW656_001999"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0837704.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            1954..2979
                     /locus_tag="IW656_002000"
     CDS             1954..2979
                     /locus_tag="IW656_002000"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0837705.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            2994..3932
                     /gene="galU"
                     /locus_tag="IW656_002001"
     CDS             2994..3932
                     /gene="galU"
                     /locus_tag="IW656_002001"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0837706.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4058..4498
                     /locus_tag="IW656_002002"
     CDS             4058..4498
                     /locus_tag="IW656_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0837707.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4501..5103
                     /locus_tag="IW656_002003"
     CDS             4501..5103
                     /locus_tag="IW656_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0837708.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5165..6268)
                     /locus_tag="IW656_002004"
     CDS             complement(5165..6268)
                     /locus_tag="IW656_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0837709.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6478..7479
                     /gene="ccpA"
                     /locus_tag="IW656_002005"
     CDS             6478..7479
                     /gene="ccpA"
                     /locus_tag="IW656_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0837710.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            7833..8228
                     /locus_tag="IW656_002006"
     CDS             7833..8228
                     /locus_tag="IW656_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837711.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            8225..8479
                     /locus_tag="IW656_002007"
     CDS             8225..8479
                     /locus_tag="IW656_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837712.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            8522..8917
                     /locus_tag="IW656_002008"
     CDS             8522..8917
                     /locus_tag="IW656_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837713.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            8917..9972
                     /locus_tag="IW656_002009"
     CDS             8917..9972
                     /locus_tag="IW656_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ0837714.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            9969..10175
                     /locus_tag="IW656_002010"
     CDS             9969..10175
                     /locus_tag="IW656_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837715.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            10187..10510
                     /locus_tag="IW656_002011"
     CDS             10187..10510
                     /locus_tag="IW656_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837716.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            10503..10769
                     /locus_tag="IW656_002012"
     CDS             10503..10769
                     /locus_tag="IW656_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837717.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(10932..13277)
                     /locus_tag="IW656_002013"
     CDS             complement(10932..13277)
                     /locus_tag="IW656_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0837718.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            13556..14458
                     /locus_tag="IW656_002014"
     CDS             13556..14458
                     /locus_tag="IW656_002014"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0837719.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            14669..16444
                     /locus_tag="IW656_002015"
     CDS             14669..16444
                     /locus_tag="IW656_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0837720.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         5441 a         2955 c         3455 g         4637 t
ORIGIN      
        1 ataattaaga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
       61 gtagtacttt tggctagaca atgataaaat ggtagagaac actctaatag aaatgaggta
      121 gcatgaatgt cagaagttgt aaaagttagc caattagtaa aaaaattatc tttggaaatc
      181 gtcacaggcg acgagaaaag tttagaccgc gtcatcgtga ctggggacat ctcacgccca
      241 ggactagagt tgaccggtta ttttaattat tattctcata atcgtttaca gctatttggc
      301 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
      361 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattgcc ggctccggaa
      421 gaaatgatcc aagcagccaa tgaaaatggg atagctgtgc tgcgttcgcc tatctcaacg
      481 tctcggctgc taggtgaatt atctagttat ttagatgggc gcttagcccc tcgaacaagt
      541 gtccacggtg tgttagtcga tgtctacgga ttaggtgtac taattcaagg ggacagtggg
      601 atagggaaaa gtgaaacggc gttggaactg attaagcgcg gacatcgatt gatcgcagac
      661 gatcgagtag atgtctatca gcaagatgaa ctgacggtaa tcggagaacc tcctaaaatc
      721 ctggaacatc tgattgagat tcgagggatc gggatcatag atgtaatgaa tcttttcggt
      781 gcgagtgcgg tacgaggctc tatgcaagtc cagttagctg tttatctaga agcatgggcg
      841 aaagataaga aatacgaccg tttgggttct gaagacacga ccgttgaaat cgcggaagtg
      901 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
      961 gtagccgcga tgaatttccg cgcgcgtaca atgggctttg acgcaacgaa aacatttgaa
     1021 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaatg actagcaagg agtgacaata
     1081 tgttagcaca aatcaatcga gttgcttttg agcttgtagg aattcctatc tactggtatg
     1141 cattgattat cgtatcagga atcattattg ccatgtggct cagcagcaga gaagctgttc
     1201 gggtaggatt aaaagcagat gacgtgacag attttatgtt gtggggattg ccgatttcca
     1261 ttattggtgc ccgtttgtat tatgttttat tcgatcttcc ccaatatata gctgatccaa
     1321 tccaaatatt caatatccgt tcaggaggat tagcgattta cgggggattg attgcaggtg
     1381 caatcacact gtactttttt accaaatatc attttatttc tttgtggacc tttttagata
     1441 tcgctgcacc aagtgtatta ctggcgcagg caatcggacg atggggaaat ttcatgaacc
     1501 acgaagcgta tggtccagac acgacacgaa gtttcttaga aagtctgcat ttaccgcaat
     1561 tcatcattga taacatgtat atcgaaggga tctatcgaca accaaccttt ctttatgaat
     1621 cactttggag tttgattgga tttattctgc ttttggtttt gcggaagaaa ccgcatcttt
     1681 tgaaaaaagg agaagtcctt ttatgttatc taatgtggta tagtttcgga cgtttcttta
     1741 tcgaaggatt acgcacagac agcctttact ttttaggtgt gatccgcatt tctcaattat
     1801 tgtctgctgt tttacttgtc ggcacattga ttctgtttat ttggcgtaga aaaaaaggaa
     1861 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatttga tttttagagc
     1921 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
     1981 ccaggctctt gggggactgc tttagcacaa gtcctcgctg aaaacggaca tgacgttcgg
     2041 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac caatcaacgt
     2101 tatttaccag atcatcatct acccgattcc atccgagcat tcaccgatat ggaagatgca
     2161 gtaaaagatg cagatgcggt actattcgtt atcccgacga aagcgatacg ttctgttgca
     2221 aaagaactag ttgccaaatt gaatacgaag ccagtgatca tccacgcaag taagggatta
     2281 gaacagggaa cgcataaaag aatctctgaa atcttgttag aggaaatccc gacagaaaaa
     2341 agaaaagctg ttgctgttct ttctgggcca agccatgcgg aagaagtagc aaagcatgac
     2401 attacgacga tcacagcggc aagcacagat gcaaaagcag caaaatatgt acaagagctg
     2461 ttcatgaacc attattttcg aatctatact aaccctgatg tggttggtgt agagatgggg
     2521 gcagctttga aaaatattat tgccattgga gctggtgcta ttcacggctt agggtttggc
     2581 gataatgcaa aagcggcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
     2641 gcaatgggtg ccaacccgct gacctttatt ggcttaagcg gagtaggcga tctagtcgtt
     2701 acttgcacaa gtgttcattc cagaaactgg cgcgcaggca acttgttagg acaaggacag
     2761 cctctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactactcaa
     2821 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
     2881 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
     2941 gggaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
     3001 taaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaagg
     3061 cgatggcaaa ggaaatgttg ccaatcgtgg acaaaccaac gatccaattc atcgttgaag
     3121 aagcgttgaa atcaggaatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
     3181 tcgaagatca ctttgattca aatatcgaat tagaaacaaa cctaagtgaa aaaggaaaaa
     3241 tcgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
     3301 ctcatccaaa ggggttagga cacgccgttc ttcaagcaag agcctttatt ggaaatgaac
     3361 cgtttgtcgt tatgctaggc gatgatatca tggaagacga agtaccattg acacagcaat
     3421 tgatcgatga ctatgaacgt acacacgctt ctacgattgc cgttatgcaa gttcctcacg
     3481 atgaaacttc aaaatacgga attatcaacc caggggaagt actggaagat gggttataca
     3541 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
     3601 ttgggcgtta cttattgact ccagaaatct ttgatgtatt atcagaacaa caaccaggtg
     3661 ctggaaatga gatccaacta actgacgcaa tcgatacgtt gaataaaaca caacgcgtat
     3721 ttgcccgtaa attcacaggt aaacgttatg acgttggtga caaatttggt tttatgaaaa
     3781 caagtattga atatggtctg acacatccag aagtaggggc acctttgcgt gaatacatca
     3841 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
     3901 taacagaaat caaagaagaa aagaaaaaat aatcattttt ttctaacgag cttcagctgc
     3961 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
     4021 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
     4081 attgatcgca gcaatcgcct ttgcgatact tgtcgttttc atcattcgcg tattgttgca
     4141 agtttccaaa actttagaaa aagtagacca gactgtcgca gaagcaaata cgacaattga
     4201 gatcgtcaca aaagacgtag atcttttatc tagacaagta gaaggcttgc tggtcaaaag
     4261 taatgagctg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
     4321 agttgctgat ttaagtgaaa gtgtatcgga acttaatcat tctagtaaaa acatcgtgac
     4381 gaaagtcggc ggaatcggaa aaacgacagc taaagcgact gttgccggaa aagtcggtgg
     4441 aacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaga
     4501 atggctaaaa aaggtggatt tttattaggt gcagtagttg gaggtacagc cgcagcaatc
     4561 gccgcattat tgttagcacc caaatctggt aaagaacttc gggatgaatt ggctgctcaa
     4621 gcagatgatt tgatggacat ggcttcagat tacacagata tggcaaaaga aaaatcagct
     4681 gagttaacag gcattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggggatcta
     4741 gcaggtaatg ttaagcaaaa ggtaagtgaa tcagcagagg aagcaggaag tgaatcagat
     4801 gatatcttgt taaacctcaa agaacaatca gctgatttat ctaatcgttt caaaaaaaca
     4861 gcagaagatc tgaaaaaacc agcagacgaa ttaggtagta ttgctaaaga tacttcagaa
     4921 gatatcatga tcgatgtaaa agatgctgca gataaagcag gagagacggt caatgaagga
     4981 atgtcagaag caaaaccagt tgcagatgac acaaaagaaa tgttgaaaat ggcaaaagaa
     5041 gatgcaaaag aagctaaaaa agaaatgaaa gatacaatga cagattcgac taccaatcac
     5101 taagaatact catttctaga ataaaaaggt gagagcggac cagctcccac cttttttatt
     5161 ttcattactc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca caaccatcac
     5221 ttgtcacata cacacaatct tcgatacgta cccctacttt ttcaggaata tagattcctg
     5281 gttcaagaga gaagcacatc ccttcttcga tcactaagtc gtttccttct acaagagaag
     5341 ggaattcgtg gacggttgtc ccaattccgt gacctaaacg gtgattgaaa tattcgccgt
     5401 agccatagct gttgattacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
     5461 ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
     5521 ggaattctgt tggtttttga taagcaactg tacgagtagc atcactgcaa tagccattcc
     5581 aaacaacgcc aagatcgaat aacactagtt catttggtga aaccttcgtg cttcctggtg
     5641 taccatgagg gcttgcagca tttgctcctg caaggactaa ggtgtcgaaa gacatttgtg
     5701 aaacaccttg tttttttaat tggtattcga tttctgcgat aattgcttgt tcagtcacgc
     5761 cttcttttac tgttttgaag ccaatttcaa aggcaacatc ggcccattta ccagcttcga
     5821 tcagtttgtc tttttcttct accgtcttga tcagttgcag acgctggata atagaagtta
     5881 gatcagtgta agaatctgca cctgtcaaga tatcagagat agcatcgaag cgatcgacag
     5941 tcagagcatt cttttcaatc cctacattac caggttgccc atagcgtgcg cgaagctctt
     6001 ttccgatgat ctcccaaggg ttttcgctgt ctaagtagcc acgtacatcg tacgcccagc
     6061 tgctgttttg agcatcttca acttccagag caggagtgaa aaggaaagga tcttgttcaa
     6121 gagggataaa aagagctagt acacgctcgt gaggatcact ttcatatccg gaaaagtaag
     6181 cgatgtggcc agggtcgctg atataagcca attctacttg gtgttgtgcc atccattgac
     6241 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
     6301 tcaataattg taccactgat tgattaaaag accaacgttc ttaaaactga aaaaagtaaa
     6361 aattttcatg aaaacagaga taaaacggtt gcaattgttg agattatttg atatcctatt
     6421 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
     6481 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
     6541 gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag caacaagaaa aaaagtcttg
     6601 gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg cacgtggctt ggcaagtaag
     6661 aaaacaacaa ctgttggggt aatcattcct gatgtcagca atatgttctt tgcctcttta
     6721 gcacgtggta tcgacgatgt agcaacaatg tacaaataca atatcatctt agcaaactca
     6781 gatggcaacg atcaaaaaga agtcaatgta ttgaataatt tattggctaa gcaagtggac
     6841 ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
     6901 aaaacacctg ttgtactagc tggttccatt gatccagatg aacaagtagg tagcgtaaac
     6961 attgattatg tatcagcaac aaaagatgca gtaaataaat tagcaaaaag cggcaataaa
     7021 aaaattgctt ttgtcagcgg tgcgctgatt gatcctatca atggtcaaaa ccgtctaaaa
     7081 ggctataaag aagcattaag cgaaaacaat ctaccatata gcgaaggatt aatctttgag
     7141 gcacaataca actttaaaga tggtctgtct ctagttgacc ggatccataa tagcggtgca
     7201 acagctgtct atgtttcaga cgatgaatta gccattggta tcttggatgg tttattagac
     7261 cgtggcgtaa aagtaccaga agaattcgaa atcatcacaa gcaacaattc tttactgaca
     7321 gaagtagctc gtcctcgttt gacaagtatc acacaaccat tatacgattt aggagcggta
     7381 tccatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
     7441 ttgccttatg gaatcgaaga aaaaggatca actaaataat aaaagatcaa tcatttagag
     7501 gttgttccaa aagtggccaa cttctggaac aacgtttatt cgagaaaagg ggttatgaca
     7561 aaattttgtc ataacccctt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
     7621 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
     7681 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agaaaagtaa
     7741 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
     7801 ccatcataca taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
     7861 tcatcatttt gaccattctt gctataggaa tcggaggaaa attttacatg gacaaaagag
     7921 aagcacaaag acagaaagat ttactcgcag tagaaaaaca atccgtcaaa gttctgaaaa
     7981 atacctttgc agatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttactg
     8041 gatgttatgg tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt
     8101 attttttttc aaaacaaatg aatgaaatta gcagttatgg tgtagaaaat gaagaaatac
     8161 aaaacgaagg ggttactact cctaaaataa gagttattta ttctaatgga gaggcggagg
     8221 aagtgtgaca gtaaaagacg aagagtatag atatataagt gaacatgttt attggctaga
     8281 tccaaaacat cctcaatata gtaaaagtta caaagaaggc gccattaaaa actttggaga
     8341 aacaaaattc caaatcctaa aaatcaaaga ctcattggat ggcatgcagg cgatggctgc
     8401 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctacaaa
     8461 ctttcgcgta cttaaataat gaaggtatca atacttacat aaaaagaaag gaggcacgtt
     8521 cttgtctcgg aaaataagaa aactcagctt agccatttta ttaatcgtca tattaggaat
     8581 cggagggaaa gtctatatgg acaaaagaga agcacaaaga cagaaagatt tactcgcagt
     8641 agaaaaacag tcagtcaaag ttttgaaaaa tacttttgcg gatatcaagg aagtgaagat
     8701 tgaaaaatct gctcgaaatg aaatgacggg agcatatcgt atgtttgtga caatgataaa
     8761 taatcacgat gagtctgttt attttacgtt tggattttgg aaagaacgaa atgaattagg
     8821 agattatggc gtagaaaata aaagtataca aaaagagggg attacagtaa ataaaatcag
     8881 agtaattttt tctaataata aggaggaata tatttaatga tggcagacaa acaatacagt
     8941 gttattagtg aggctgttta ctggctagat cctaaacaca gagactatga tcatacttat
     9001 aaagaaaatt ctattaaaaa aattgatagt tataatttta aaatcctaaa aatcaaagac
     9061 tcattggacg gcatgcaggc gatgggtgta gcgccaatca agaacggtaa agtggatctt
     9121 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
     9181 gatgcaagga gtatcatgaa tctctatggg aatagaccag gagcaagttt ttctgaaaaa
     9241 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
     9301 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
     9361 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
     9421 gtcggcttaa acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg
     9481 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
     9541 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
     9601 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
     9661 gaaaatgaaa ataatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
     9721 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
     9781 cggctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
     9841 aagttatcag caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
     9901 atttttgagc tagagaaaaa atggcatggc aagaaggctt gcggcaagca cgtgttgtcg
     9961 ccccttcgtt gactgaacag gaaatcatag aagcattagc tagtgttgtc gccacaaaag
    10021 agaacttagt cgattctgcg aaagaagtct atctgctgcg aatcaataag gcaagacgta
    10081 tgggggaggc ctttgataca ttggtgaagg agatccaaga caaaatcaat gaaatcgtga
    10141 cacgagatcg agaattagca cagcaattca attaaaggag aagtagatga aactagaaga
    10201 attggaaaga gattatcgaa gtgaacgaag acaattagaa gctcaagaag atgaattgat
    10261 ccactttaag aatcaaggat tacgattgat ggagcaagag gagcagcgac tgcatcatgt
    10321 attaaagaac ttagctttag ataatgagcc tctgtttcaa gcaagacgaa gttattcgaa
    10381 ttatcaggag cattatcaac aagccctaca agtagaacga cgaaaactct atcagcagca
    10441 agacgaacta gaacgttcct atcgtcaaaa aatcagacaa ataactatgg agaaatcatc
    10501 ttatgaatga atttttaatg caattactaa aagaattaga gaaaaatcta catcgatcaa
    10561 atgtacgttc agttggtatg gggatatctc agcaaatttg ggcagaaaaa cgattgacag
    10621 atcaaaacca ccaaatgact gcagaagaag ctgctcaaga attttctaac cgacatgtac
    10681 acttgaaaga atcagtaaaa gggcaatttg cagaaaaact aaccgaatta ttccaaagac
    10741 aagaaaagaa attaaaacag tttcgttagt aaagttatat agaaaaattt tgtgaaaatg
    10801 gcttttcaca tttttacata aatttgtctt aatatcgtag atgcaagctt ttgaacacca
    10861 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
    10921 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
    10981 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc aacgccgaat
    11041 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg attccactgt ttttcctggc
    11101 gtttggatag attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
    11161 acttttttct tcatcacatc agatgataag tcaaatttct catcagtgcc gaaaatctca
    11221 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtatcctt
    11281 gcttggctgt ccattccagt attgtcatcg tgtcctgccc agctactgat agtgatagac
    11341 ggagtagaga cgatcagcca tgaatctctg tattcgttgg tagaaccagt ttttccgacc
    11401 caatctgctt ttcctagatt tccgtttaag cttgaaatga catctttgaa tggtgtagtg
    11461 attttttcat tgatcacact gcgcatcatg tcattcatga tcgaggccgt tgcttcggaa
    11521 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
    11581 gaatcaatga tatagccttc ttcgtagacg ccaccattag ctaaggcctg gaatccattt
    11641 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag cagattcaac gccccaatta
    11701 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcatattg tttcgatcca
    11761 cccgaattca atacgtcttg gtataagtga taagctggga tattgttgga ccattccagt
    11821 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctcg
    11881 ccagcatttt ctccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
    11941 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
    12001 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg gccacccacg
    12061 aatccataga tacgtccagt cctattgtcc atcaatacat ttccagtctc cacttggcta
    12121 ccagcaccat catctaacat atagccgtaa ttttctacgc cggtttgcaa tgcagcatag
    12181 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
    12241 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
    12301 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
    12361 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
    12421 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
    12481 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtagggacta
    12541 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatct
    12601 tttgcatcta caccaaagat tcctcttgca gcttcctgga ctccagcaat attttgcccc
    12661 ttattattcc gaccaaatgg cgagacattc aaatacgtag caatgatctc atcttttgaa
    12721 aagtattttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
    12781 tcatcggtta ggatctgctg tttgactaat tgctgtgtca aagtggatcc tccagacgag
    12841 ccgatgccag tagcttctga aactaatgct cgtaaaacag ctttagggac ataaccttgg
    12901 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
    12961 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
    13021 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
    13081 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
    13141 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
    13201 ctaaaaataa ccttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
    13261 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
    13321 aataataaga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
    13381 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagagaaa
    13441 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
    13501 ttaccaatat ataggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
    13561 attaggctca cacgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
    13621 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
    13681 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
    13741 aacaaatatc gtcgttcatg cgccctatat cattaatttg ggaaatacga tcaaaccaga
    13801 aatgtttcca tttgctgtac aatttctgag agaagagatt gttcgtgcgg aagctttagg
    13861 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aaggtgggat
    13921 tgctagaatt attgagggtt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
    13981 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aactagcgca
    14041 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
    14101 gaatgatgcc ggatataata taaaagaaga tttctcaggt gtgatggaag agtttgataa
    14161 ggtcatcgga ttagatcggc ttaaagtact ccatatcaac gattctaaaa atccacaagg
    14221 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
    14281 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atcttagaaa ctccatatgt
    14341 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaattgcca tgttcaaagc
    14401 gcaagcattc gatcccgaag tgtttcctga gttattagca gaagaagtca cactttaagt
    14461 tttctatctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
    14521 aaacaaagga gtgctttttg cagtcgtttt cgccatgctc ctttcgcccc ctctttttaa
    14581 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagattc acaagcaagt
    14641 agcaaaacgt gattgaaagg acgaaaagat gctgaacact ttaaaaccct tgatcaacca
    14701 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
    14761 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcaacct atgatgatca
    14821 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtacacgac
    14881 tacgacactt tcaaaaggga tctcctacag cttgttttta gtacttgcac ataaactgca
    14941 tctttcttat gcagttttgt tcggcatgtt cagtattctt gccagcatat tggttgcact
    15001 agcgtttaaa ccgatcgtga agaacaagta tcttctttta agtatctatt tttatttttt
    15061 gttttctccg attagtttta cacatgagta ttccactcga acgtatcgta atgctctcgt
    15121 gatcccggct gttgagatca ttatcgcttg tttactagct atttattacc gtaaatatag
    15181 ttcccgcaga aaattgctgc cgtggttttt agggttagct gtttcttttc ctttcttttg
    15241 gttcattcgc gaagattcca tctggcttct tccttttact gtagtcgcgt taggcatcag
    15301 tttcgttcag atcttgctag gacaagactt ttctttcaaa gaagcaaaga agttaaaaga
    15361 acgaccattt ctcctttcga aacaagaatg gctgaaactt tcattgtttt tactccctct
    15421 agtcggtttc tccatcgtct atcagtggat cgaatataaa aacgagacaa catatggtat
    15481 tgccaccatc aatgaccgtt cgagtggtga atttggacgt ttgatgaaac agctgatccg
    15541 aatagatgac gaaacgaact taaataaaga aaacagtgag atatgggtct cgcatgctgc
    15601 attaaataaa gcattagacg catctcctac atttcatact gtttctactc gtattgacga
    15661 attgtatacg actcatgcgt ggacccaagg cggaaaagta aaagaattac caggagatat
    15721 cattttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
    15781 agaaaccaat gatttttgga aaaaagtcaa tcaagaatta gaagaagcct atcaaaaggg
    15841 aactatcaaa aagaaaaaag aattctatct gatgggatca ggggatggaa agacgatgaa
    15901 tgatctgcca atcgtatttg cttttctgaa atcaggatat gcctatgcat ggcgatacaa
    15961 agatttccat caaggaggga attatagttt cgggacgcaa gaacaagtcg aacaggccca
    16021 agaattattg aatcttccac taatggataa ttggatcgat agcagtgatc cgagaacacc
    16081 tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc atgataaaaa tttaccaagt
    16141 tctggccatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
    16201 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtag tcaccggatt
    16261 aatactgaca caattggttt ttcttttcgg tgtctcgtgg ttttgttctt atgcgccaga
    16321 aaaaaaggat tattttttaa gtgtctacac tggcgcaggc gtgccattaa cacaattcat
    16381 cgtagtaaca agcatgctag ttttcagtaa attgaatctg aaaaaagctt tatcttctaa
    16441 ataaaaaggg aactgtgaca aaagtcttgt cgcagttcct ttttccga
//
LOCUS       DACEJZ010000068        16064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEJZ010000068 DACEJZ010000000
VERSION     DACEJZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.071"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            49..164
                     /gene="rrf"
                     /locus_tag="IW656_002016"
     rRNA            49..164
                     /gene="rrf"
                     /locus_tag="IW656_002016"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(217..687)
                     /locus_tag="IW656_002017"
     CDS             complement(217..687)
                     /locus_tag="IW656_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0837721.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(846..1304)
                     /locus_tag="IW656_002018"
     CDS             complement(846..1304)
                     /locus_tag="IW656_002018"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0837722.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1475..2619)
                     /locus_tag="IW656_002019"
                     /pseudo
     CDS             complement(1475..2619)
                     /locus_tag="IW656_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2632..3549)
                     /locus_tag="IW656_002020"
     CDS             complement(2632..3549)
                     /locus_tag="IW656_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0837723.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3977..4540
                     /gene="efp"
                     /locus_tag="IW656_002021"
     CDS             3977..4540
                     /gene="efp"
                     /locus_tag="IW656_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0837724.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5026..6285
                     /gene="lysA"
                     /locus_tag="IW656_002022"
     CDS             5026..6285
                     /gene="lysA"
                     /locus_tag="IW656_002022"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0837725.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6272..7072
                     /gene="dapF"
                     /locus_tag="IW656_002023"
     CDS             6272..7072
                     /gene="dapF"
                     /locus_tag="IW656_002023"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0837726.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7075..7956
                     /locus_tag="IW656_002024"
     CDS             7075..7956
                     /locus_tag="IW656_002024"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0837727.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7953..8669
                     /locus_tag="IW656_002025"
     CDS             7953..8669
                     /locus_tag="IW656_002025"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0837728.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8675..9370
                     /gene="dapD"
                     /locus_tag="IW656_002026"
     CDS             8675..9370
                     /gene="dapD"
                     /locus_tag="IW656_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0837729.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9386..10387
                     /locus_tag="IW656_002027"
     CDS             9386..10387
                     /locus_tag="IW656_002027"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0837730.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10384..11745
                     /locus_tag="IW656_002028"
     CDS             10384..11745
                     /locus_tag="IW656_002028"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0837731.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11705..12856
                     /locus_tag="IW656_002029"
     CDS             11705..12856
                     /locus_tag="IW656_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0837732.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12831..14000
                     /locus_tag="IW656_002030"
     CDS             12831..14000
                     /locus_tag="IW656_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0837733.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14175..15554
                     /locus_tag="IW656_002031"
     CDS             14175..15554
                     /locus_tag="IW656_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0837734.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15835..16064
                     /locus_tag="IW656_002032"
                     /pseudo
     CDS             15835..16064
                     /locus_tag="IW656_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5252 a         2837 c         3427 g         4548 t
ORIGIN      
        1 tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg tggtggcgat
       61 agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct tagcgccgat
      121 tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc aaaaagatga
      181 actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt caattgcagc
      241 ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc catttctatc
      301 aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag gagcttcatt
      361 tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca attgatgcat
      421 agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg ctgaatctag
      481 ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg tctgataagc
      541 ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg ttgcagtgtt
      601 aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc cgtcttctaa
      661 agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta tataataaaa
      721 agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt tcagacaata
      781 aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt acgttctttt
      841 agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc acgagccaaa
      901 gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc attgcttgca
      961 ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc taaaatgtct
     1021 tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc cattggagac
     1081 cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg ctcaagtgaa
     1141 tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac gtcgtattta
     1201 atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata aggtgctttg
     1261 acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa gactcctatc
     1321 tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa tttacaaaaa
     1381 cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct gaaatatttt
     1441 ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg tcaaatgcct
     1501 gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga cggatcaagc
     1561 cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga gtcatgacag
     1621 aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta aatagttgga
     1681 gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa atcattccgc
     1741 taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt aatcctggat
     1801 agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca aaagcatttt
     1861 tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt aaccagctat
     1921 cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga tcggctagat
     1981 cttttggtta gaagtcacta aaccagctac tacatcgctg tgtccgccta gatatttggt
     2041 accgctatgg acaacgatat cagaaccgag atctaaaggt ctttgaaaat aaggagtagc
     2101 aaatgtattg tcagcaataa ctatcaatcc atgttgatga gccagagtgg cacttttttc
     2161 taaatcagta atctttagaa gcggattgct aggtgtttca agataaagtg cttttgtatt
     2221 gggtttgatg gattgttcta ttttatcaag attactcgta tctatgatcg tgtactcgag
     2281 accattttta gtaagaactt tgtcaaatag acggaatgtt cctccataga catcatctcc
     2341 taataagata tgatcgccag cttgaaaaag agaaaaaacg gcatgaatcc cagaaagacc
     2401 agaagaaaaa gcgaatcctc gaacaccgcc ttccaaatca gcaatcagtt cttccaatgc
     2461 aaaacgagta ggattaccag atcttgaata ttcatattgt ttaggctggc cgacgccatc
     2521 ttgccgataa gtggatgttt gataaatagg tacactgaca gctcctgttg tgggatcttc
     2581 actgatgcct ccgtggatca attttgtttg aatgtgcatg tttctttcct cctataagta
     2641 aatgtttttt gataaatagc ggtctgctgc gtctggaaaa atcgtaacga cacgatgacc
     2701 aggtggtaaa cgacgaattt ctcttaatgc tgctgcaaaa gccgcaccac tcgaacttcc
     2761 aactaatagt ccttgttctc gtgctaactg cctagtatag ttaaaccctt ctacatcaga
     2821 gattgtctcg atctgattga ttgaaagggg agaaagaaac ggcggaatga attctacacc
     2881 gataccctca atttcgtgag gggctggatc accaccattt aacacagagc cctctggttc
     2941 tacaccgatt attcgaatgt cgggatattt ttctttcaaa aaggtagaag ttccagcaaa
     3001 ggttccacca ctcccaattc ctgctacaaa actatggata ttttctttga tttcatgaaa
     3061 gatttctggc cctaacgtat gatagtaagc agcaggatta tctttatttt caaattgaag
     3121 aggcagatag ctgtttgata tctcctctgc aagtttttta cttttgctga tggcaccgat
     3181 gattccttgc tcactaggag aatggatgat ctttgctccc aatgctttca tcaattgttg
     3241 tttttctaag ctaaacttct ctggaacaac aaaaaccgtt cttaatttgt atgtgagtgc
     3301 ggccagagcg atcccaattc ctgtatttcc ggcagttggt tcgatgatcg tcgttttttc
     3361 attgattttt ccttgggcaa atccttcttt gatcaaataa ctgcccaatc tatctttgat
     3421 acttcctcca ggatttaaat attctagttt tgcataaata gcagaacctt taggcataga
     3481 atagtgctga ttgtcaaagc gataaagcgg tgtggaacca atagcttctg taattgtcga
     3541 gataatcatg tgggcctcct aaaaaaagat aataaaaaaa ggagctgact attaaatcag
     3601 ctccttgaaa gacatagaga aaaattgagt agataaaaag ataagttacc tggtactcat
     3661 tctatgaagg gcaattaata gacatgacaa atagtatgct gcttttatgc accatacaac
     3721 agcaacgatt caattgtttt tggttgctga ctgtcatgtg tatccctcca agtaaaattt
     3781 ttattaacga tagcatgaaa aaaaataaaa gtcaaaggct tgaaagaaaa aaatatttcg
     3841 atagcatcgt ttacccgtgt ttatttttct gacaaatagg catgagacct taaaagatag
     3901 agaatactct agttttctgg ccggttgtct gatacaataa agttgattat gataaagaaa
     3961 agaaggagca aaaataatga tctcagcaag tgatttaaga gccggtatga ctttcgtaca
     4021 agacggcaaa ctgattaaag ttctggatgc aagccaccat aaacctggta aaggaaatac
     4081 agttatgcgt atgaaactga aagatgtccg ttcaggtgca acatacgaca caacgttcag
     4141 accagaagaa aaatttgaaa aagcacatat cgacacaaaa cctgtacaat atttgtacac
     4201 gatggatgat acagctttct tcatggattt ggaaacatat gagcaatatg aaattcctgt
     4261 agaaacagta gaagaagaaa tgaaatatat cctagaaaac atggaagtaa aaatccaatt
     4321 tttcggtagc gaagtcatcg gcgttcaatt accaacaact gtcgtattac gtgttgtgga
     4381 aacacaacct tccatcaaag gtgcaacagt cactggttca ggtaagccag cgacaatgga
     4441 aactggctta gtagtcaatg ttcctgactt catcgaaaca gatgaattgt tggaaatcaa
     4501 tacagcagaa ggttcttatg taaaacgtgc gtcaaaataa ttcgctaacg gattgataag
     4561 aatttgaaaa cagagcttga cctcttgaaa ggcagatcat cggatctgcc tttcaagagg
     4621 ttttttgtgg aatcagcaaa ttgaaaaata aaaaagtatt tgattttatt tatattttga
     4681 aagatgaatg gcaaaagcta gagaaagaaa aaactgcact tatttatgaa gaaattgaaa
     4741 tgattcataa aattgaaaat atccgaacta ttccgaacta tattgaagcg aaaatatttt
     4801 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
     4861 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
     4921 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
     4981 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
     5041 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
     5101 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
     5161 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
     5221 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
     5281 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
     5341 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
     5401 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg
     5461 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
     5521 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
     5581 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
     5641 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
     5701 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
     5761 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     5821 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     5881 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     5941 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     6001 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     6061 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     6121 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     6181 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     6241 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     6301 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     6361 tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt
     6421 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     6481 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     6541 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     6601 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     6661 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     6721 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     6781 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     6841 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     6901 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     6961 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     7021 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     7081 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     7141 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     7201 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     7261 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     7321 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     7381 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     7441 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     7501 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     7561 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     7621 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     7681 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     7741 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     7801 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     7861 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     7921 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     7981 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     8041 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     8101 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     8161 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     8221 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     8281 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     8341 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     8401 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     8461 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     8521 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     8581 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     8641 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     8701 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     8761 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     8821 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     8881 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     8941 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     9001 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     9061 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     9121 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     9181 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     9241 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     9301 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     9361 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     9421 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     9481 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     9541 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     9601 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     9661 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     9721 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     9781 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     9841 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     9901 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     9961 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagaaccg
    10021 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
    10081 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
    10141 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
    10201 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
    10261 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
    10321 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
    10381 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
    10441 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
    10501 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
    10561 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
    10621 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
    10681 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
    10741 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
    10801 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
    10861 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
    10921 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
    10981 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
    11041 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
    11101 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
    11161 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg
    11221 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
    11281 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
    11341 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
    11401 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
    11461 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
    11521 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
    11581 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
    11641 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
    11701 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
    11761 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga
    11821 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
    11881 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
    11941 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
    12001 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
    12061 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
    12121 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
    12181 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
    12241 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
    12301 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
    12361 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
    12421 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
    12481 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
    12541 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
    12601 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
    12661 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
    12721 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
    12781 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
    12841 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
    12901 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
    12961 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
    13021 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
    13081 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
    13141 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
    13201 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
    13261 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
    13321 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
    13381 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
    13441 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
    13501 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
    13561 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
    13621 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
    13681 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
    13741 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
    13801 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
    13861 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
    13921 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
    13981 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
    14041 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
    14101 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
    14161 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
    14221 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
    14281 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
    14341 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
    14401 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
    14461 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
    14521 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
    14581 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
    14641 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
    14701 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
    14761 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    14821 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    14881 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    14941 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    15001 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    15061 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    15121 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    15181 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    15241 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    15301 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    15361 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    15421 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    15481 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    15541 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    15601 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    15661 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    15721 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    15781 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    15841 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    15901 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    15961 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    16021 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DACEJZ010000069        15595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEJZ010000069 DACEJZ010000000
VERSION     DACEJZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.072"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1218
                     /locus_tag="IW656_002033"
     CDS             <1..1218
                     /locus_tag="IW656_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0837735.1"
                     /translation="NSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSM
                     FVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPY
                     EIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLF
                     SLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDILI
                     ILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNY
                     PSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIA
                     INLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYP
                     TQLLDKIEFDYNI"
     gene            1418..1732
                     /locus_tag="IW656_002034"
     CDS             1418..1732
                     /locus_tag="IW656_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0837736.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1753..2130
                     /locus_tag="IW656_002035"
     CDS             1753..2130
                     /locus_tag="IW656_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0837737.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            2140..2913
                     /locus_tag="IW656_002036"
     CDS             2140..2913
                     /locus_tag="IW656_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837738.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2935..4338
                     /locus_tag="IW656_002037"
     CDS             2935..4338
                     /locus_tag="IW656_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0837739.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            4520..5704
                     /locus_tag="IW656_002038"
     CDS             4520..5704
                     /locus_tag="IW656_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0837740.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5701..5991
                     /locus_tag="IW656_002039"
     CDS             5701..5991
                     /locus_tag="IW656_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837741.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5988..6209
                     /locus_tag="IW656_002040"
     CDS             5988..6209
                     /locus_tag="IW656_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837742.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            6251..7030
                     /locus_tag="IW656_002041"
     CDS             6251..7030
                     /locus_tag="IW656_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0837743.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            7097..7591
                     /locus_tag="IW656_002042"
     CDS             7097..7591
                     /locus_tag="IW656_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0837744.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            7647..8285
                     /locus_tag="IW656_002043"
     CDS             7647..8285
                     /locus_tag="IW656_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837745.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            8357..8752
                     /locus_tag="IW656_002044"
     CDS             8357..8752
                     /locus_tag="IW656_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0837746.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8736..11189
                     /locus_tag="IW656_002045"
     CDS             8736..11189
                     /locus_tag="IW656_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0837747.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            11186..13213
                     /locus_tag="IW656_002046"
     CDS             11186..13213
                     /locus_tag="IW656_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837748.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            13210..14232
                     /locus_tag="IW656_002047"
     CDS             13210..14232
                     /locus_tag="IW656_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0837749.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            14249..15178
                     /locus_tag="IW656_002048"
     CDS             14249..15178
                     /locus_tag="IW656_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0837750.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            15423..15539
                     /locus_tag="IW656_002049"
     CDS             15423..15539
                     /locus_tag="IW656_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0837751.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5317 a         2576 c         3069 g         4633 t
ORIGIN      
        1 aacagtaacg ttgcacgtaa tattgctagt aatataaggg cgatttatgt ggatgaattt
       61 caagatacta gagaattaca atacaatgta atagctaaat tacttcaaaa caaccctcaa
      121 attcagtcaa tgtttgttgg agatattgac caagcaattt atggatcatt agatggtata
      181 gctaaatcag ttgaagaact agaaatgcta actagtcaca cttttcaatc aaagacgcta
      241 catggctgtt acaggtctaa tcagagatta gtagacttct actctaattt tcagagttgt
      301 ccttacgaga ttgaaagcag agggaataat aaaaacgacc gtggttttat aagttatgat
      361 tgcaaagtta ctaaggagga tttacccaac ataataaaaa atataataaa ggaaaagatt
      421 gaaagcggca ttcatgaaaa agatatttgt gtacttgctc ctcaacacta tcctctgttt
      481 tcactgggtg agtatttaaa gaaagaatta ccgtattgca attttgatgc tataaatata
      541 tcaccaatta aggtaaataa tcatagttta tttttcaagt tagcaatttt gtactttact
      601 gaatcaggtg aaaaggttcg aagaagaaag ctaattgcaa acgatatttt aattatatta
      661 aaaaatgaat ttggaataga agttaaagat tattttattg acttagattt attacagcta
      721 ataaacagtg tgaaaagaat gtatagtgga gacgacaacg gtgttgattt atacaaattt
      781 gttaccaaac atatatttgt taatcttaat attgacgaga aaaattaccc gtctattttt
      841 aagcactatc tattttttat taacgaagta gaagatcgga taaaaaataa ttcattatct
      901 agtagtgtat ctagttttaa gaagatgttt aaatctcgag atggtatcac acttactaca
      961 gcacataaag ttaaaggaaa tgagtatgat actgttatcg ctattaatct tctaaacggg
     1021 aaaattcctc attggaatga aatttttaat gtttctgata aaggaattag ttcagcgaga
     1081 aaactactat acgtaatttg ttcaagagca aaaaataatt tatttctttt ctcagaggtg
     1141 ggttatttta cgagtaaagg agacttgctt tatcccactc aattattaga taaaatagaa
     1201 tttgattata atatttaaaa tacctaggta gtcgttgttt taaatatagt aggaatcgga
     1261 cgctcagtac ggagcgtcta tttttttacc caattttgaa aggaagtgaa cttatgaaaa
     1321 taaaaaatca agaatcaaaa ggtcgctctc ccccctttaa aaccatcaca caaataagat
     1381 tcattcaaac ataaaaacag aaaggatagg taaaaatatg gaacttaaat atgtcattcc
     1441 caatatggaa aaaacatttg gacaattaga atatgctggt gaagggaaca ttgaacaacg
     1501 tcgaataaat ggcagaccaa ccattctctc tcgtagttac aatttatact caacgattca
     1561 acgagcagat gatattgttg tcattcttcc ctccgaagct ggcgagaaac attttgaatc
     1621 cgacgaaaaa gtcaaactca tcaacccaca tattaccgca gaaggttata aaattggcaa
     1681 tcgaggattt accaattaca ttatgcacgc agacgatatg gtgaaagcat aagaaaggaa
     1741 aggtgaaaaa atatgagatt agcagaaggt attgtgattg aaaaagacca gacatttggc
     1801 acattgaaat tttccgcctt gcgtcgtgag gttcgtatcc agaatgaaga tggtacggtt
     1861 tccgaagaaa ttaaagaacg tacctatgac ttaaaatcaa aaggacaagg acgcatgata
     1921 caagtcagta tccccgcttc tgttcctttg aaagaatttg actacaacgc agaagtggaa
     1981 ttaatcaatc ctgtggcaga taccgtagcg acggctactt ttcgaggggc agacgttgat
     2041 tggtacgtga aagcagacga tcttgtatta aaaggacaga caaaacctca tcagaaatat
     2101 aaaaaagaag cgtccgaaag tgaaaaatag gtgttgctta tgacaaacac accattgatt
     2161 ttgaaagaaa ttccgaagga tgaagccatt tcttttattc ggcaatacca ttattcaaag
     2221 atactcccta gattgtgtaa atactttcta gggatttttt cagaagaaaa attattaggc
     2281 gttgtggaat taggctgggg tacgcaaccc ctacaaacca tacgcaaact ctttcccgac
     2341 agttcccttc aaaccacaga ttatttagaa attggaaaga tgtgttttct tccagaaatg
     2401 aatcaaacca attattttgg cagtcaagct ctttcagctc taattaagtg gttgaaagaa
     2461 catacggact gtcatttttt atatacactg gcagatggaa ttgagggaaa atgtggctat
     2521 gtctatcaag cgtccaactt cttttactgc gggtatttca agacaagtgt ctatcgtgat
     2581 aagcagtcat gggaaaagat acaccctcga agtgctcgcc ttttattaga ggaaaatgct
     2641 cgctttgaac aggtagagaa aaagcattgg ctttcccagg cattttgcga atacaaaggg
     2701 attgagaaaa tcaatggacg catgtttcgc tatctctatc cgttgacaaa agaagcgaaa
     2761 aaactgttag gacacactct ctatcgacgt cattattatc cgaaagaaaa aaatttacgc
     2821 tttgaaaagc gaatcgctta tcagaaatat gaagccattt ctcaaccaac ttttgataaa
     2881 caggctcgta tctacaatac acaattattt tgataggtgg aaaggaggat ttttatgcca
     2941 ttgcttcatg acagaggaga gcgaatcaaa gccaacgata agcacttggt ctatcatttt
     3001 gtactaattt cattgctggt agtttttcta gtcgtcctac tcccttttca ttggagagcg
     3061 ttacaatcgg tgaactggca agcagttcag ttagatacat ttttctatca acttcatacg
     3121 ccatactttt ggagcagtat tctcactgct ctttttgtat gcggacttat cagctggctt
     3181 tattatcact atcgtataga ttctataaaa cagttagagc accgtcaaaa gttagctcgt
     3241 atgattttag aaaatcattg gtatgaagcc gaagaaactc aatcagaggg attctttaaa
     3301 gatttaccca gcaagaaaac caaacaacga atccgccatt ttcctaaaat atattatcga
     3361 atgaaagatg gactaatcca tattcatgta gaaatcacct tagggaaata tcaagatcag
     3421 cttttgcacc tagaaaacaa actagaaaca ggattgtatt gtgaattagt ggaaaaaacg
     3481 ttgaaagatt cttatgtaga gtatgtccta ctttatgata ctattggcaa tcgaattggc
     3541 attgaagacg tggtagctca agatggacgt gtcaaattga tggaatcggt ctattgggag
     3601 ttcgattctc tccctcacat gttaattgct gggggcactg gtagtggaaa atcctatttt
     3661 attctcacat tgattgaatc tctcctgcat acaaatgcaa agctttatat tcttgaccct
     3721 aaaaatgctg atttggcaga cttaggggcg gtcatgaata atgtctatta ccgtaaagaa
     3781 gatatgttgg catgtatcaa tcaattttac gaagatatga ttgatcgcag tgaaaccatg
     3841 aaacaacacc ctaactataa aacaggagaa aattatgctt atcttggttt gtctgccaat
     3901 tttcttattt ttgatgaata tgtggcaatg atggatatgc taggtcgtga aagtaccacc
     3961 gttattcata aactcaaaca aattgtaatg ttaggacgac aagcaggctt ctttttaatt
     4021 cttgcctgtc aacgtccaga cgcaaaatat tttagtgatg ggattcggga caactttaac
     4081 ttccgtgtgg ctttgggtcg tatgtctgaa atgggctttg gtatgatgtt cggaagcgat
     4141 acacaaaaac aatttttctt aaaacccatc aagggaagag gttacgtgga tacaggaaaa
     4201 agtgtgatta gtgagtttta tacacccctt gtaccaaaaa ggtacgattt cttaggagaa
     4261 ataggaaaag tcattcaaaa aaagcaatct gagcctgtac cgcacgaagt gaaaggtaca
     4321 ggttcagact tgctttgatt ggtgtggcgt tagccacgcc aatcctttag ccccctctat
     4381 ctaacagggg ggcacaaata caactgaaaa caggcaattc tcatcaacca atgttgatgg
     4441 tacaaaggat tgcggaaaat atgaaaggtg taaactcaag ggtcatagtt tacatctttt
     4501 ttagattgga ggtttgcgaa tggaaaacga aaaaatttgg attaaggaat tgaaagaaaa
     4561 gcgactggct tatggcgttt ctcaaaataa actggcggtt gcttctcata ttaccagacc
     4621 ttacctatcg gatattgaaa caggcaaggc agtccctact caacaggtga aagaagattt
     4681 actgaacgct ttggaacgct tcaatcccga taacccattg gagatgttat tcgactatgt
     4741 acggattcga ttccctacca atgatgtggc tcaaattatc ggtgaggttt tacgtttgaa
     4801 tatggattat atgctacacg aagactttgg ctattattct tatcccgaac actatcgttt
     4861 cggtgatatt gttgtcctag tctctcatga tgtaagtaaa ggtgttttat tagaactgaa
     4921 aggaaaaggc tgtcggcaat ttgaaaactt tctattggct caacatcgga gctggtatga
     4981 tttctttcaa gattgcatgg aacataaagg tattttcaaa cgcttggatt tagctatcaa
     5041 cgataaaaca gggattttga atattccaga tttggcaaag aaatgtaaac aggaagaatg
     5101 catttccgtc tttcgaagct ttaaaaatta tcgcagtggt gaattggttc accgtgatga
     5161 aaaaccatac atgggaaaca cactttatat tggctcatta aaaagtgaag tctatttctg
     5221 tatatatgag aaagattatg agcaatacgt gaaaaatgat gttcccttag aagacgcaga
     5281 agtaaagaat cggtttgaaa ttcggttaaa aaatgaccga gccacccaag ccatgaaaga
     5341 tttattggca catcaacagg cggaaaaaac agcctttgaa attatcaatc gctatatccg
     5401 ctttgcagat aaagacgaca ctaaacgtcg gagtgattgg aaaactaatg aacgttggga
     5461 atggtttatc ggtaaaaatc gtggagcatt acgcctaacc acacagccag agccttattc
     5521 ttttgaacga acattgaact ggttacatca tcaagtcgcc cctacattaa aaatcgcaag
     5581 cattttagat gttctcaacg gtacaaccat tatctccacc atgattcagg aagccaaact
     5641 gacagaaaaa catgagaaat taattgaaca acaacatcta gccatggagg atttgattac
     5701 atgagccttt taacaaaaga attaaagaag ttagggtttc agtgtggcat tgagtttcaa
     5761 gcgtatatac aaaatacagg gaaatatacg tctctcatta ttgagggaaa acggcaagct
     5821 ggtgatacca tctataccta tgatttttac aaggtgagat tttataataa ttataccaat
     5881 cgagtaaccg tctatggaga gcatttaacc ccttttcaat tattaaggcg tgtaaaaagc
     5941 tatatctatt atcgagaaaa atatcttaag gaaaggagga caatcacatg aattttgggc
     6001 agaatttgta caactggttc ttggataatg cacagtcatt ggtattgtta gcgattgttg
     6061 tcattggact gtttttaggt tttaaacgag aattttcaaa actcattggc ttcttgatta
     6121 ttgctctaat cgccgtcgga cttgtgttca atgcagctgg cgtgaaagat gtgttattaa
     6181 acttatttaa ccgaatcatt ggtgcttaac agatattttt catgataaaa taggaagtat
     6241 atattttgtt atgaaaggat ttatgaatat gtttacaaat gatcaattta aatctctttt
     6301 aattaaaaca ttagaaagaa agagctcttc aaattattat gaaggaggta acgagattgt
     6361 ttctaaaatg aaaatatcag aggtaacttt agatgagact gacgatttta cttattataa
     6421 aggatataaa aagggctgga atacgctttg cacatatctt aaaataagag ttccttttga
     6481 tgatttagac ttttttgaaa gtcataaaga tttgattacc cagacagcaa gctccatata
     6541 tgataaacaa ggtgataatg tattagtgga tactatttta gtaccacttc cagagaatta
     6601 cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag ctattgaaga
     6661 cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag ttcacactgc
     6721 ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc ctagagacga
     6781 aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa ttcaacctac
     6841 tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga ttagctctct
     6901 taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta ttggaaagag
     6961 agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta gcggatacct
     7021 tgataagtag cgtcattaac tgattgctac tttttttatg actattttta taaaaaggat
     7081 gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata atgaaggtga
     7141 attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg
     7201 tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt ttgatgtgga
     7261 cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg
     7321 tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta gcttggaaga
     7381 attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga tggaagatgt
     7441 cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca atttacaaaa
     7501 ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt acctagttac
     7561 ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat aattaaatta
     7621 ataaatagca cggagaggta taaattatgg taaatgtatt tgaaaatctt aaactattaa
     7681 aactagacat gaaagataaa ggctgggtga tagattcctt ttactttcgt tacaaacagc
     7741 aaaattatat tgtacttgtt aagctatttg aaaaagagga aaaagttcca gaatatgctc
     7801 tgttaaaact agaattttta aaggaaaacg atttttcaga tatgctagct gtctatgcta
     7861 attcggtaaa attatataca gacacgaaaa caataagaga atattttggt atcgaataca
     7921 gtagtaactt gggagatgtt ttatttcaat ttagccaaac tcttgctcga tttattccga
     7981 cagaagttag tgagaagaaa aatgaagacc aaaaagaagc catgtgtttc tcattaagtc
     8041 aaagtgattc agaagatccc agaaaaaaat attgtttttc agttagaaga aatcctttaa
     8101 agggaaatgg tgagctggga aaacgaagtc cttttaatga caataaaacg agattgtata
     8161 gacccagctt atatgaacga ttagggtcag ataccaattt aagtttcaga tattcaatga
     8221 atccagatga tgaagaaact gatgaaggta ttattgcaaa atggacaaaa aataaaattg
     8281 aatagttaaa gcattgaaag agcaatcacc tattttgatt gttctttttc tatttttacg
     8341 gaaaggacgt gaagacatga aaaaaataag aagttacacc agtatttggt cagtagaaaa
     8401 agtgatttat gcgattaatg atttacaact ccctttccca attaccttta cgcaaatgac
     8461 atggtttatc gtttctttgt ttgtcgtcat actctttggc agtttaccac cactttctct
     8521 gattgacgga gcactactca aatacttagg gattcccatt gttctcactg ggttcatgtg
     8581 ccaaaagacc tttgacggaa agaaacccta tggatttcta aagtctatca ttacctatgc
     8641 tctacgccct aaagtaacct acgctggcaa acaaatacga ttaaaaaaag aaacaatgaa
     8701 tgaatcgatt acgattgtaa ggagtgaaca actagatggc atatccgatt aaatatatgg
     8761 aagataatct cgtatttaat catgatggcg aagtattcgc ttattatgaa ttaattccct
     8821 ataattacag ttttttaagt tccgatgaaa aaattcaagt tcatgataat ttctgtcaat
     8881 tgattgccca aaatcgtgat ggcaagattc atgctctgca actagcaacc gaatcaagta
     8941 ttcgagccac acaggaacaa tcgaaaaaag aaatcactgg acgattaaaa gagatagctt
     9001 acgaaaaaat agatgaacag accgaagcgt taatttctat gattggtgac aatcaagtag
     9061 actatcgctt ttttattggt tttaaactct acctcaatga acaggaagta tccgtaaaaa
     9121 atgttgggaa agaaattcaa aaagcatttg cggaattctt tcatggtgtg aatcacaaat
     9181 taatgggcga ttttgtttct atgcctatgg cggaaatcga acgcttttct aagatggaac
     9241 agttgttaga aagtaaatta tccagacgct ttcagattcg taaattggag aaagatgatt
     9301 ttggttatat tattgaacat ctttacgggc aaacaggtgt cccttttgat gactacgact
     9361 accatttacc gaaacgatac gaagataaaa aagtgttggt aaagaaatac gatattctca
     9421 aacccactcg ctgtttaatc gaagaaaatc aacgctattt acggattgaa caggaagata
     9481 ccactactta tgtggcgtat tttacaatta atagtattgt tggtgaattg gatttcccta
     9541 atagtgaaat tttttattac cagcaacagc agtttacttt ccccgttgat accagtatga
     9601 atgttgaaat tgtcgccaat aaaaaagcct tatctaccgt tcgcaataaa aagaaagaac
     9661 tgaaagattt agatagccac gcatgggaaa gcgacaacga aaccagtact aatgtggtgg
     9721 acgctttaga atctgtcgat gaattagaaa gtaatttaga ccaaaccaaa gaatctatgt
     9781 acaaactaag ctatgtggta cgggtatcag caaagaattt ggacgagctg aaacaacgtt
     9841 gtgatgaagt aaaagatttt tatgatgatc tgtctatcaa attagtacga ccttttggcg
     9901 atatgctagg cttgcataat gaatttattc cctcaagcaa gcgatatatc aatgactata
     9961 ttcaatatgt cacgtcggat tttttggctg gtcttggttt cggtgctaca caaatgattg
    10021 gtgaaaattc tggtatttac gtcggttaca atctcgatac aggcagaaat atttatctcc
    10081 aacctagcct tgcaagtcaa ggaattaaag gctctgtaac aaatgcatta gcgtccgctt
    10141 ttattggttc attaggtggc ggaaaatcat tctctaacaa tatgctggtc tattattcag
    10201 tactctttgg cggacaggca accattgttg acccaaaagc tgaacgtggt gaatggaaag
    10261 aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt gagaacgaca
    10321 ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca gaaagccttg
    10381 ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag aagtttcctg
    10441 tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg ttattggtga
    10501 ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac catatcgaaa
    10561 gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc acccaatcca
    10621 ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta cccgattcca
    10681 atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg ctgattgtca
    10741 ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa attgttgacc
    10801 ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct atgcgattgg
    10861 ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac gcggacgatt
    10921 tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt tccactgacc
    10981 tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat gaaaacaatc
    11041 agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc tatggtcgtg
    11101 taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt gataccagac
    11161 cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc ttcactatag
    11221 ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc tgggcaatgc
    11281 agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat actccaagta
    11341 tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct ggcttccatg
    11401 gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca ttactaattt
    11461 tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga aagaagccta
    11521 tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc agacgattgc
    11581 aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc tccttctttt
    11641 tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag aaacgacgaa
    11701 agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt cctttattgc
    11761 gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca gtaccgccag
    11821 tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga aagacagtgt
    11881 ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc tattgcttca
    11941 atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt tattgtctac
    12001 cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag aaattgaaga
    12061 tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa cagtcttctt
    12121 tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag gaatcatgat
    12181 tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt tcctattaag
    12241 tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat ttaatgtgat
    12301 tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt ccactatgct
    12361 ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa ttgtgacctt
    12421 tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac aaagcaacga
    12481 ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga atcgacaaac
    12541 cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt caacgaagca
    12601 aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc gtcaaaatga
    12661 acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac aaggcggtat
    12721 cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc gtctagtcaa
    12781 taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca aatatgccct
    12841 tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat ctatggcaga
    12901 taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac gaaagaacat
    12961 tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag aaaacccatc
    13021 atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg tcacgcctgt
    13081 tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag agcaaaaaca
    13141 aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg ataagcgagg
    13201 acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc tctcttcttt
    13261 tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg cgaaaacaac
    13321 aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt ggcacaccgt
    13381 ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc ttatatctta
    13441 gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag ttccgaatct
    13501 ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg ggttaaatat
    13561 ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc tgttatccaa
    13621 tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa aaaatatacc
    13681 tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc agactatccc
    13741 aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa tcagttttat
    13801 gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga tgatgaaacc
    13861 gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt ctttggaggg
    13921 gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta tgacaaagct
    13981 ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga aatctctatg
    14041 gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc aggcacatat
    14101 gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg cgacccaatc
    14161 ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc tagacgtgtt
    14221 attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa aagaaaagaa
    14281 ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga tgattaaagt
    14341 tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta gtgtcggatt
    14401 tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa cagaaatcat
    14461 taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct ttgcgaaaga
    14521 ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa aattgaccca
    14581 ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg atattcctac
    14641 tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg agaacacctt
    14701 tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg aaactatatc
    14761 gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta ttatcaaaaa
    14821 tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc tagaaagtga
    14881 ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa cgttcttcca
    14941 gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc atgtgttacc
    15001 tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta ccagaaaaga
    15061 taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa aggcaaccca
    15121 aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg taaaataaca
    15181 aatattggta catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg
    15241 agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt
    15301 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg gaacaaataa
    15361 ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc
    15421 atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata
    15481 aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa
    15541 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgc
//
LOCUS       DACEJZ010000070        15314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEJZ010000070 DACEJZ010000000
VERSION     DACEJZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.073"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            278..787
                     /locus_tag="IW656_002050"
     CDS             278..787
                     /locus_tag="IW656_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0837752.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IW656_002051"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IW656_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0837753.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IW656_002052"
     CDS             2322..3302
                     /locus_tag="IW656_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0837754.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IW656_002053"
     CDS             3424..4842
                     /locus_tag="IW656_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0837755.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IW656_002054"
     CDS             4913..6382
                     /locus_tag="IW656_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837756.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IW656_002055"
     CDS             6392..6859
                     /locus_tag="IW656_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0837757.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IW656_002056"
     CDS             6895..7767
                     /locus_tag="IW656_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0837758.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IW656_002057"
     CDS             7849..10383
                     /locus_tag="IW656_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0837759.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IW656_002058"
     CDS             10539..11312
                     /locus_tag="IW656_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0837760.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IW656_002059"
     CDS             11305..12096
                     /locus_tag="IW656_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0837761.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IW656_002060"
     CDS             12106..12597
                     /locus_tag="IW656_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0837762.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IW656_002061"
     CDS             12615..13025
                     /locus_tag="IW656_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0837763.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IW656_002062"
     CDS             13029..14429
                     /locus_tag="IW656_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0837764.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IW656_002063"
     CDS             14452..14826
                     /locus_tag="IW656_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0837765.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DACEJZ010000071        15015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEJZ010000071 DACEJZ010000000
VERSION     DACEJZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.074"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..1398
                     /locus_tag="IW656_002064"
     CDS             7..1398
                     /locus_tag="IW656_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0837766.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="IW656_002065"
     CDS             1414..2454
                     /locus_tag="IW656_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0837767.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="IW656_002066"
     CDS             2473..3558
                     /locus_tag="IW656_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0837768.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="IW656_002067"
     CDS             3591..4553
                     /locus_tag="IW656_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0837769.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="IW656_002068"
     CDS             4546..5979
                     /locus_tag="IW656_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0837770.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="IW656_002069"
     CDS             5976..7046
                     /locus_tag="IW656_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837771.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="IW656_002070"
     CDS             7033..8454
                     /locus_tag="IW656_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0837772.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFITIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="IW656_002071"
     CDS             8467..9474
                     /locus_tag="IW656_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0837773.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="IW656_002072"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="IW656_002072"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0837774.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="IW656_002073"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="IW656_002073"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0837775.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="IW656_002074"
     CDS             11608..12285
                     /locus_tag="IW656_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0837776.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="IW656_002075"
     CDS             12275..13417
                     /locus_tag="IW656_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0837777.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="IW656_002076"
     CDS             13430..14407
                     /locus_tag="IW656_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0837778.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..>15015
                     /locus_tag="IW656_002077"
     CDS             14407..>15015
                     /locus_tag="IW656_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0837779.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSN"
BASE COUNT         5427 a         1916 c         2680 g         4992 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattacgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaat
//
LOCUS       DACEJZ010000072        14841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEJZ010000072 DACEJZ010000000
VERSION     DACEJZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.075"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            419..1033
                     /locus_tag="IW656_002078"
     CDS             419..1033
                     /locus_tag="IW656_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0837780.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1806..3545
                     /gene="yfcC"
                     /locus_tag="IW656_002079"
     CDS             1806..3545
                     /gene="yfcC"
                     /locus_tag="IW656_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0837781.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3702..4886
                     /gene="iadA"
                     /locus_tag="IW656_002080"
     CDS             3702..4886
                     /gene="iadA"
                     /locus_tag="IW656_002080"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0837782.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4997..5200
                     /locus_tag="IW656_002081"
     CDS             4997..5200
                     /locus_tag="IW656_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0837783.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5301..5963)
                     /locus_tag="IW656_002082"
     CDS             complement(5301..5963)
                     /locus_tag="IW656_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837784.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5976..7478)
                     /locus_tag="IW656_002083"
     CDS             complement(5976..7478)
                     /locus_tag="IW656_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837785.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8026..9705
                     /locus_tag="IW656_002084"
     CDS             8026..9705
                     /locus_tag="IW656_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0837786.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9832..10311
                     /locus_tag="IW656_002085"
     CDS             9832..10311
                     /locus_tag="IW656_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0837787.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10530..11210
                     /locus_tag="IW656_002086"
     CDS             10530..11210
                     /locus_tag="IW656_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0837788.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11218..12549
                     /locus_tag="IW656_002087"
     CDS             11218..12549
                     /locus_tag="IW656_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0837789.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12564..13526
                     /locus_tag="IW656_002088"
     CDS             12564..13526
                     /locus_tag="IW656_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0837790.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13637..13975
                     /locus_tag="IW656_002089"
     CDS             13637..13975
                     /locus_tag="IW656_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0837791.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14054..14761
                     /locus_tag="IW656_002090"
     CDS             14054..14761
                     /locus_tag="IW656_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0837792.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3193 g         4308 t
ORIGIN      
        1 gtttttttgt attttcttgc ttgcacttgg ataagagatt acttacaata aattcagata
       61 gaaacagctg aataaaaaaa ggctccctga cccttagttg gcgctaagaa tcagggaatg
      121 accagtcaca cacatacacc ctgaccgatc attttgccat agtcaatgca gtgcatcgac
      181 ctaatttatt gtacctaatc tagggtgaaa attctacccc tatgtagata ttttccatgg
      241 tctgcacgtt gttttatttg ctatgtcaag gtattgggcg tattcgttca atgccttttt
      301 tgtgtttgaa acagcaaaga ccacatgtgt tactgtctaa ctactgtttt tttatgtact
      361 tccttagatt aggcagctgt ttctattcat gtcaagtata gctaggcggg agagataaat
      421 gagtttatgt tattttgact attatcacca attggaagag aaattaagga tagaagagcc
      481 gctgatgctt caagctggca cttggatcgc gaaacaaatt aatcaaggtg gtagattgca
      541 gatatatgct agccgcacac taagtggtgt agcatttgaa ttttgggatc aaaccccgga
      601 tcttgtacca agtatattga ttgaaaatcc agccggaggt gtctatgaaa cgctagaagg
      661 tgcaggacag gcaattattg atcagctttc tgtcaaatca gaagacattt ttcttcttct
      721 ttcgaatgaa ggaagaaacc cggcaatcat cgaactagct cagtggatca aagaaaatgg
      781 tcatccatta atcattgtta ctggatttga tttgtcacga agtatcaaat cgcaacatag
      841 cagtggtttg cgattatttg aatttgctga tttggttttg gacaaccacg ccaatttaca
      901 tgatgcagtc ttgactttgc caaatctaga tccagcgatc tgcggaacag cctctcttgc
      961 aacgatcttt ttgctgcagc aagttttgta ttttacagca aaacagctag tccaggacac
     1021 aagtggaaaa tagttatcca catgttgaca aagttcctta aaaaaataat gttccacata
     1081 aaaatggagc gtttgaaaaa aagaaaacga ttattaatga ggattaacaa cgactacttt
     1141 ttttgtttta gttattttaa gatggatcat ctttgatttt aagcttgttt tgtttaaagt
     1201 ttgttatttt tataagttgt ttcacagtta tgagaaatga gttataagaa tatgtagtgt
     1261 cagaaaaata aaaagtagtg gaaaaagaga caagaaagaa tccatatgga ttctttcttg
     1321 tctcttaata cacgttgatg aaagagtctt ccccaaaact ctttacaaag taattatagt
     1381 acatttgtta tcattattgc aatgaagttt acctgatata acaaaattgt ccaacaggat
     1441 acctttttct aatagaaata aaatacacaa aaattgaagg atagataatg gatatttata
     1501 agtgtttatt attttttttg caatgaaatt caatttttca atgattgtaa aatgaaaaaa
     1561 acaggacgag attttctcta atctaaaaat aaaaaaagaa ttttaaggaa aaacagagaa
     1621 ataaaatact taatataaat ggaaagagaa aaaaataatc atacatagga gaaaaaaaac
     1681 aaaatagaaa aaagtgattt tctaataaat tattcatgtg gaaatgtcat ttttactgta
     1741 taattcattt gattatccat tttttattca tcaaaattac tgaaaaaaga aaggaagaaa
     1801 taaatttgaa gaaaacaaat tcttcaggaa aaccgaaacg cgacttcact aaattatcga
     1861 caccccatac gtatgtcatt atttttggtg tagtcatctt tgcatggata ctgacttttg
     1921 tcgttccagc aggaaagttc agcacgcaag atattgaata caaagatgca aatggcgaaa
     1981 caagtactcg tactgtttta cgtcaagatt ccttccgtta tgcttacgag ctggataagt
     2041 cgtatgtgtt tgatcaatta gaagaactgc aagatcatcc agcagaacgt gaaaaactgg
     2101 atgttccgga aaaaggattg gaaaaagtca ttgctgacgg agaaaaaaat cttacgcagg
     2161 aaaaactaga tgaaatctcc ttgacagatg atgtgcttta cgatcagtac ggcgaaaata
     2221 tctatgatac ttctaaaaaa ttgcataaaa cagccaaaat atggggaaca gatgattttg
     2281 gcggctttgg tttcttaaat tttgtttttg aaggactagt atctggagat aagtatggat
     2341 ctgctgtagg gatcgctgca ttgatccttg ttgttggagg tgcttttggt attattatgc
     2401 gtaccggagc gatcgacgca ggtatctatg cgtttatcag taaaactaag ggactggagc
     2461 gtttagcttt accgctatta ttctttgctt tttcattcgg tggcgctaca ttcgggatgg
     2521 cagaagaggt aattccattc tcaatggtga tggttccatt tgtcattgcg ttaggatatg
     2581 actcgatcgt agcagtaacc gtgacttatg tcgcatctca agtcggaaat gcgacctctt
     2641 ggatgagccc gtttagcgta gcggtggctc aagggattgc tggaatcccc gtgttgtctg
     2701 gtgcgacatt ccgattgatt atgtgggtag ttgttactgc attagccgct ggttatatga
     2761 tgatatatgc agaaaaaatc cgtaaaaaac cagaaagttc gttgacttac aaatcagatg
     2821 attatttccg ttctcatatc aaaaagacat ctgatgaaaa taaaaaattc atgcttgggc
     2881 ataaattgat cttattagag atgttagttg tccttgtctg gatcgtttgg ggcgtgactc
     2941 aaaaaggcta ttacattcca gaaatcgctt ctcaattctt tgtgatggga ttagttgctg
     3001 gaatcattgc tgttcttttc aaattagatg gaatgaaagt caatgacatc gcttcttctt
     3061 tccaatctgg tgcagctgat ttagctggta cagcaatcgt agtaggtatg gccaaaggga
     3121 ttcttcttgt cttgggagga tctgatgcat ctgttccttc agctttgaat acgattttgc
     3181 atgggatcgg gacggcgttg accggtgttc cagctgtcat tggtgcttgg gcgatgtaca
     3241 tcttccaaag tttgttcaac ttggttgtta catcaaattc aggacaagca gctttgacta
     3301 tgccaatcat ggctccactg gcagatttag taggtgtatc cagacaagta gcagttcttg
     3361 cttatcagct tggttcaggt tttatggacg cttttacacc cgtatcagcc agcttgattg
     3421 gtgtgctagg tgttgcacgg atcgaatggg caaaatgggc aagattccaa atcaagatgc
     3481 aaggattcct gtttgtttta ggaactattt tcatcatgat tgccattgca ataggtctgc
     3541 agtagtactg gagacctgta aataaaaaag taatccaaac agtagataag gactccaaga
     3601 accgtcattt tacggtaaaa tcaaaggagt aaagatccta tcgtttggat tattttttaa
     3661 gatcctatca tcagttatac ttaaagaaag aggaaaaatg tatgaaaatc attcgacaaa
     3721 ttgaagtgtt tgcaccagat tacttaggaa aaatggatgt actgattgcc ggtgggaaga
     3781 ttttggctgt agaagaacag ctagaaggcg gctacgaagg agtggaagta gaagaacttt
     3841 caggtgaggg aaaagtacta accccgggtt tcattgactg tcatttccat attttaggtg
     3901 gaggaggaga aggcggttat caaaatcgaa cgccagaagt cacattgagc caattgacta
     3961 cagctggtgt aacgacagtt gtcggttgtt tgggaacaga cggagaaggt agagatatga
     4021 ctgcgttgat cagcaaagcg aaaggtctgg aagcagaagg aatctccact tatgtatacg
     4081 aaggttcgta ccgattgccg gtaaaaacag taacagattc tatcatcaaa gactttttga
     4141 caattgataa aattatcgga atcggagaaa tcgcagtatc cgatcaccgt tcttctcagc
     4201 caagttttga agagttcgct cgtgcagtag cagatgctcg tgtaggaggc atgttatccg
     4261 gaaaagctgg gatcatcaat gtccacttag gcggaggaaa aagaaagcta gagctattga
     4321 cacgaatcgt agaagaaaca gaaattccga tcacacaatt tttgcctact catgcaaatc
     4381 gcacaccaga attatttgaa gcttgcgtgg catttgccaa acgtggtggc acgattgact
     4441 tcactgctag cgaagatcct gatttttggg agaagacaga tggtgaagtt cgcttcagca
     4501 aagcactgaa acgattgatc gaggaagatg tttctttaga taatttcacg atgacctcag
     4561 atggacaagg aagcttgcct tattttgatg aaaacaatca tttcctaggg ttaggagtag
     4621 gaagtgccaa ggcattgcta gtagggatca aagaagctgt gcaaaaagaa agtatccctt
     4681 tggaaatcgc cttacgagct ataaccagta atccagcacg tatattgaaa ttggataaaa
     4741 aaggaaaaat cgaaattggt gctgatgcag atctgtgtat cttggataaa gaaacgcttg
     4801 atattgatac agttatcgct aaaggagaaa taatggtcca agaaaaagaa gtcaaagtat
     4861 gggggacttt cgaaaaaagt ttttaatcaa cagctctaac tatctttttt agaaaacgag
     4921 cgttctgttt gtctgtatct gttgttgatg ttaccctgaa ggtagatcaa taaacaaact
     4981 aagaggagga agcacaatgg cagatttaaa aggacgttct aaagatatga aagataaagt
     5041 agtcggtgaa ggaaaagaag catacggcaa agcaacagac gacaagagca aagaaacaga
     5101 agggaaagca caatcactag gtgctgacat caaagaaaaa gctcgaaatt taggcgacga
     5161 agtcaaagac aagttttctc gaaaagacga aaaaaaataa aaaccactta ttactaattg
     5221 aatcaagcaa ataaaaaatc cgaaccatat ctgcgagaca gatatggttc ggatttttta
     5281 tttgatcatt atcctgtttt ttaaacttcg attttttgaa attttttatt ttttagttca
     5341 aagacttcat cacagctttt tgctacttca atctcatgag tcacgatgat aatacagcga
     5401 ttttgttcat gggcgatttc ttggaaaagt ttgacgatat cttgtgaagt tgtttcatct
     5461 agatttccag taggctcatc cgctacgatt agttgatgtt cacagcagat agcacgaacg
     5521 atagcgactc gctgctgctg acctcccgaa agtttggtca cctgtttttt tgctaatatt
     5581 tcatcaattc ctacacgagc cagtcgatct aaagcaactt gtcgataatt ctcttgcttg
     5641 gtcttagtga tttccattgc tgctatgacg ttgtctagag ccgataaata aggaagcaag
     5701 ttgtaagctt ggaaaataat cgagacatca tttttacgat aacttcttaa cccgattttg
     5761 ttcagtgaac gatcttgata gtataccttt ccttcttttg gtgcatctag tccagaaata
     5821 agcgaaagaa aagtcgtttt tccagaacca cttgctccta aaatagcgta catttttcca
     5881 gcttcgaaat tcagttctac atctttataa agcgcatctt gcggatcttg ataccaaaat
     5941 cccaattgtt ctgtccgtaa catcaaattc ctcctttagt ttaataggat ttttcttggg
     6001 tttaatctta atatccctat agaagctaaa aagacagaaa acagactgat tccaaatgct
     6061 actccggcta atagtcccaa ttgtgcaggt ttgacattga tttccaattc gctgacttga
     6121 tcagaaacag caaatggatt attcgtggtt ccttgttgac catcgcgttg accgccttgt
     6181 tcttgtctgt tacctggcat ttgtccaccg ccaggagctt gctggttttg tgtcgtggtt
     6241 tctgtctgtt ggtcaagtag ctgctgtccc acggcattcg cgacgacgtt tccgctaagc
     6301 gaagcaactc ctaatgcaag aatcatacaa atagccactt cggtaaacaa ttggagaatg
     6361 attttacttc tggattctcc taaagatagc aagacgccta actcatgccg acgctcccga
     6421 atggtaatca taatgatcag agtaagtatg ataataccgg ctacagccac tagtaaaaca
     6481 acatttttag aaaaacttgc cacgttgttt aaaggttcca acattgactg atacatggaa
     6541 tcatttgatt gtaagctaaa tgtatctgtg tcgatcagct tctcagcttc tttgagaaac
     6601 tcatccattt tatctggatc agatagagtg taaatagcag aatctatcgt gttatcgtca
     6661 gatgtcccat ttagttcatt tgcaaaagtg taagaagtgt aaagtgtatt cgacggattc
     6721 atgaagttga atttcatacc catactgccg cttgtttcgc tggattcata aattcctttg
     6781 atcgtcattt cataagtggt atcttcatct tcagtactcg tgattgtgaa agtatctccg
     6841 actgccaggt catttgtttc tgctagagta gaatcaatga ctacattgtt tgtgcctttg
     6901 tcgctgtcat ctattccttc accatccgtg atttttgctg ttccatcaga aaaattacta
     6961 ttttgtgcag ttgctgacac accactgatt tgaaagtctg cttgagtcat ttgcggattc
     7021 ccgcccattc ctccagcagg ttcaccgcct gcatcagtag tttctgaact gtcttcactt
     7081 gaatcagaac tagtgatggc agttattcca tcttttgcca tagctgaggc ggaactttca
     7141 aaggaataac tctttacatt atctaattca gctatttttt ccgcgtccgt taaggaaact
     7201 ggtgtaagag aaaaactgcc tggatctgga cgttcttcag tggtagaacc gcttgaagaa
     7261 gtttgttcct gttttttaaa tgaagcttct cgattggcac ttaaagtcac tgttgctcca
     7321 acttcttttt gtgcttgatt cgctgcttct tctgcagcgc ttcgaatcgt gaggccagct
     7381 agcacaaaaa taagaatggc tgtgaacacg gcaaacaata acaatgaacg tccccatttt
     7441 acttttgtac ttttccatgc acgtttcata aaattcatat tttgcatccc tcctagtaga
     7501 tgcatttgat aatagacaac ctatcttaaa gctagcttaa atcttatgct gtttgcacac
     7561 catcaacatg aggttctgta tatataaaaa gtcactatag ttattgcaaa aaatgtggta
     7621 aaatgaaaaa agtatgtttc aaaccgacga agaagaaatg aaagaggaat agcaaaagaa
     7681 atcctagatg ctacagaggg atttttacgt gcatagttgt tctaacaatt gctgatacta
     7741 gcgatttagt ttatttgttg tataataatg gggaacagct gaatacttat ataagtattc
     7801 tggctatagc gtttcgcaaa agatccattt tgttaatgaa gtatatccaa aataaaatgc
     7861 ccatccaact gtcagaacgc ggatgtgctg gtaattttcc tgctgattct ccataagttg
     7921 agaaatagtg attttgcata tgcttcattg gaatggctca tatatttaga taaagaagaa
     7981 aatcgacatc tttaaacttg gatgtcataa gaagaggtga agtttgtgag taccattaaa
     8041 attatcccat taggcggcgt tcgtgaaaat ggaaaaaaca tgtatgtcgc agaagtgggc
     8101 gaggatattt ttattttaga ttgtgggcta aaatatcctg aaaatgagtt gttggggatc
     8161 gacgtcgtaa tccctgattt tacgtattta gaagaaaaca ttgatcgtgt agctggtgtc
     8221 tttttaacac atggacatgc agatgcgatc ggggcacttc cttacttgtt gtcaaaaatt
     8281 tctgtaccag tattcgggac agagttgacg atcgagttag caaaattgaa tgtcagtcgt
     8341 aatgaagcag ccaaaggatt taaggatttc catgtggtag atgagcatac agaaatagac
     8401 tttggtgaaa cagtagtgag ctttttccgt accactcata cgataccaga ttcagtgggg
     8461 atcaatctga aaacaaaaga aggtagtatc gtttatacgg gagattttaa atttgaccaa
     8521 acggctatcc caatgtatca aacagattat gcacgattgg cggaaatcgg caaagaagga
     8581 gtcttagcct tactaagtga atcggcaaat gcagaaaatc ctcagcctgt agcttctgaa
     8641 gcccaaatag cagatgaagt atttgatacg atcaaatatt gggaagggcg tatcatcgtc
     8701 gcttgtgtag caagtaattt gcaacgagtg caacaagtat tggatgcagc agaaaaagca
     8761 caacgaaaag ttgtcttgac tggtcaagat ttcgaacgaa ttattcgcac agcgatgcgt
     8821 ttagaaaaac ttcaattacc aagtgaagat ctgcttgtta cgttgaaaga catgaaaaac
     8881 tatgatccag aagaactgat cattttagaa actggccgaa tgggtgaacc catcaagtct
     8941 ctgcaaaaaa tggcaaacgg tacacaccgt aatgtcaaga ttgaagaagg agatttggtt
     9001 tatatcacta ctacgccaag catcgcgatg gaaaccatcg tggcgaaaac ggaagatatc
     9061 atttatcgag ctggtggaac agtgaagttg atctctgaga atatgcgcgt ttctggccat
     9121 gccaatccta atgacttgca attaatgatc aatttgatga aaccaacata ttttgtgcca
     9181 gttcaaggag agtatcgtga attagctgct catgcagatt tggctcatgc agtagggatg
     9241 ccatacaaaa atatctatat tactggacgc ggagacgtgc tggaatataa gaacaagcgt
     9301 atgagtgttg caggaactac aagtgcagaa aatgtaatga ttgatggatt aggcataggt
     9361 gatatcggaa acattgtttt acgtgatcga aaaatcttgt cagaagacgg gattttcgta
     9421 gcggttgtca ctatcagtcg gcgagaaaag aaaatcattt caaaacctca aattacttca
     9481 cggggattcg tttacgtaaa agctagccgt gatctgatga aagaaagcag caatatgatt
     9541 gaaacaatcg ttgagaaaca tttagaaagc aatgattttg agtggagcaa attaaagcaa
     9601 gacatccgag atcaattagg ccgttactta tttgaacaaa caaaacgccg cccagtgatc
     9661 ttgccagtca ttatggaagc tactcaacgt cgaaataaaa aataaacaaa aagagtaaga
     9721 tgaacctctc tatagagtgc tcgtcttgct ctttttttgc gaaaaacaaa taatttttga
     9781 atacagcatc cctcgcttta taataaacga tagaataaag ggagtgaatc aatgatgagt
     9841 caaacaaaag tcgaacgtta tttagaagaa caaaagatca gctatcaacc acttgatttt
     9901 agtcatgtgg tagaagaatc tttcacagaa gaactacaaa aaagagggat tgatccagct
     9961 ttgatatgta aaactctcgt agtgaaagga aacaaaactg gggtgatcat cgcattgatt
    10021 cctttgaacg atcacttaga ttacaaaaaa acgagacaag tgacgaaaga tcgtaaagtt
    10081 ggttttcctg gaatggattt tgtattaatg catacaggat atccccacgg agcaaacacc
    10141 cctattggaa taaaactcgc acatcctgat tattgttttt tagcagacag ttctttaaaa
    10201 gaaaaagaag aagtaattgt gtcaagcggt gaaatcggga gaagccttcg gataaaagta
    10261 gccgatttag aaaagatcgt ccatcctatt tacgctgatt taattagtta agggacgaga
    10321 agagaagcta agcagagcag agacctcaaa cttgagatta tatctgcttg gtacttggtg
    10381 catcctgtat tatctgttac aattgctttg tacatactgt actatcaatc caatgaaaat
    10441 atatttagca gctatatttt ttcattgatg tgcaagtgat gaaatcgttt ttaataaaaa
    10501 agatacagag aaaacgcgag gtgtaaaata tgaaaattac gtatcatgga cattcctgtt
    10561 taacaattga aatggaaaat ggccaacgcg tgatgattga cccgtttatt acaggaaatc
    10621 cattgacaga cttaactgca gaagaagtaa agacagattg gttgctgatt actcatggtc
    10681 atagtgacca cataggagat atgattccta ttgcaaaaga aaatcaagcc acaattatca
    10741 gtattgttga gattgctgat tatgcgaaca gcagaggagt agattcattc ggtatgaata
    10801 tcggtggaaa acatgcgttt ccattcggcg tggtaaaatt cgtacatgca cagcatagtt
    10861 caagctatga agtagacggt atcgttcagt atatgggcga gcctagcgga attattattc
    10921 aagcagaagg aaaaacgatt tatcatgcag gggatactgc ttatttcagt gatctaggat
    10981 tgcttgcaga agaattcgat attgatgtcg ctttcttgcc gattggcgat aactatacga
    11041 tggggccaga agatgcagca aaggctgcgc aaacaattcg tgccaagaaa gtagttccaa
    11101 tccattacaa tactttccct gtcattgaac aagatcccga atcttttgta gagttactgg
    11161 aagatagtga aggaaaaata atgagcgtct cggaaacgct ggaagtgtag gcagaaaatg
    11221 gcgacaaaac atgatttgat tttagaatat attgaaagtt tacctgttgg caatcgaatt
    11281 tctgtaagaa gcatcgcaaa agaattgaat gtcagtgaag gaacagcgta ccgtgcaatc
    11341 aaagatgcag aaaatgtcgg actagtttca acaattcaac gagtagggac catccgtatc
    11401 gaacgcaaat taaaaaaaca tattgaacgt ttgacatttg gcgaggtcgt caggatcatt
    11461 gaaggggacg ttttaggcgg ggcggcagga ttagacaagg tcttgaataa attcgtcatc
    11521 ggcgcgatga ctaaacatgc catggaacgc tatattacgc caggatcact aatgatcgtc
    11581 ggcaaccggg cggaagtcca aaaattagca ttggaagatg gcgccgcagt attgatcaca
    11641 ggaggattca atacgactgc tgagattgca aagctagctg atgatcttga aatgccgatc
    11701 ttacgcacct cttatgatac atttacagtc gcaaccatga tcaaccgagc gttaagcgac
    11761 caattgatca aaaaagatat catgcttgtc agtgatatct atatgccagc tgaaaagact
    11821 cattatttgc agcagacaga tacgatttct gattatcagc gtttgtcaga agaaacgcag
    11881 cattcgcgtt ttccagtcgt caaccgccat catcgtttga tggggattgt tacggcaaaa
    11941 gatgttttag ggaaatcgcc aaaccagatc atcgatcggg tgatgacgaa agaaccaatc
    12001 agtgtaaaga aaacaatgag tattgcctct gttagccatc aaatgatctg ggatgggcta
    12061 gaagtcatgc cggtcgtttc cgatgacttg acattggaag gcttgattac ccggcaagat
    12121 gtaatgaaag cgatgcagct tgtccagcga caaccgcaga ttgcagatac gatttcggat
    12181 cagatatctg gaaatatcca tacgattgat actgaccgtg aaggaaatcg tttggaaaac
    12241 cctaagttca aattcgtagt agcaccacaa atggtcaatg gcgttggaac catttctttt
    12301 ggcgtactta gcgaaatcat ttctgatgtc gcacaaaaaa caatggtgat gaaccaaaaa
    12361 cgaaatatct tgattgaaca agtgaaccta cattatttgc ggctgatcca attggaaagc
    12421 gaattagaca ttcgtccacg tgtattggaa atcggcagac gttccgctaa actggatatc
    12481 gaagtgttta ttgaaaacac catcgtttcc aaagcaatcg ttgtttgtca agtaatggaa
    12541 cgttcttaga aagcaggagt gaaatgacag tacagcaaga aatagtagat acaataaaaa
    12601 aatacgataa aatcattatc cacaggcatt tgcgccctga tcctgatgca ttaggatcac
    12661 aagtaggatt agccgaacta ttacgggaaa gtttcccaga aaaagaaata cgtcaagtag
    12721 gtgaaacgat tgaagggctt cgctttttag cagaaatgca agaagttgaa gatgtcttct
    12781 accaaggagc gctagtcatc gtgactgata cagctaattc acccagaatc agtgatcaaa
    12841 gatatcagtt ggcagaaaaa ctgatcaaaa tcgaccatca tcccaatgat gacccttatg
    12901 gagatcttgt gtgggtaaat acaaaagcaa gtagctgtag tgagatgatt gcagaatttg
    12961 ctttgatgtt tcctgaatct ttaaagatga acgttaaggc tgcacggtta ctttatgcag
    13021 gaatcgtcgg agatacaggg cgctttttgt atccctccac gacgtcccgt acattagaga
    13081 ttgcttcgat attgagaaat tatccatttg atgcaagtgc tttgaacaga gagattgaac
    13141 aaatgccgat gaaagtcgct aaactttccg gttatcttta tcaaaatatc caggtggatg
    13201 aaaatggtgc aggaagaata atccttccgc aagaaattct tgataaatat ggaatcgatg
    13261 attcagagac agcttcgatc gtttcacttc ctggagtgat tgacgaagtg ctggcttggg
    13321 ggatttttgt tcagcagcct tcagggtatt accgcgtacg tttacgttct aaggggccga
    13381 tcatcaatga attggctaaa cgccatcatg gaggaggcca tccattagca agtggtgcga
    13441 atgcaaaaga tttagcagaa gtagaggaaa tctataaaga gatccaagat atatgccaat
    13501 cgtataagag cagaaaaaca gaatgatact tcgttcgaaa acgtgccttt tgtagtaaaa
    13561 atcacaaatg tatcttttca agatcttctt tttccgttac aatagtgctc actaaagatt
    13621 ttaaaggaga aagaagatga cattaccaaa ttgcccagaa tgtggctcag agtacacata
    13681 tgaagatcga ggcttgttga tttgcccaga atgcgggaac gaatggaaca aagaagaaca
    13741 agaaactgtc atagaagacg gattagtggt attagatgca aatggaaacc gtctgttaga
    13801 gggcgatagc gtgacagtga ttaaagactt gaaagtaaaa ggtgcgtcag gtgccattaa
    13861 acaaggaaca aaagtcaaga acatccgttt agtagaaggc gatcataata tcgattgcaa
    13921 ggtagaagga tttggaccga tgaaactgaa atcggaattt gtgaaaaaga attaaagaat
    13981 attcaaggtc ttttcacatt cttttcctac tactaatgat aaaataaagt aaacgaaaag
    14041 gagtgaaacg aaaatgtatg gttatggaat gtatggtttt ttagatccga catttctttt
    14101 ggtcattctt ggattagtta tttccggaat tgcgtcagcg tacgtcaata gtactttccg
    14161 aaaatacgat caagtgagaa gcaaaaacaa tgtgactggt acacaagcag cacaatatat
    14221 attacaaagt caacagataa ataatgtcgg tgttcaacaa attgccggag atctcacaga
    14281 taactacaat tcaggtaata agatgttgag tttgtctcaa gcaactgcac agtctacttc
    14341 agttgctgcg ataggtgtag cagctcatga atgcggacat gcggtacaag atgcttcggg
    14401 ttatgtccca ttgaagattc gtgcagcact tgttccagca gctaactttg gttcgatgat
    14461 ttcgttcccc ttgattatgg taggtgtatt gcttagctgg aactctacgt taatcaatat
    14521 cgggattttc gctttttctt tagcattact tttccaattg gtgacattac cagtcgaatt
    14581 taacgcgtct cggcgagcaa tccaaatttt gagtgaaggc ggactactga cggaagaaga
    14641 agtaccgatg gcaagacacg tattatttgc agcagcactg acttatgttg cagcagcgct
    14701 ttcgacattc ctgcagctgt tgcgcttgat cttattgttc ggcggcaacc gaagagatta
    14761 aaataaagaa gttgtgaaat cagcgctttg atctgagcta ataccgagag ttagctgatt
    14821 gaataccatt tatttggaat a
//
LOCUS       DACEJZ010000073        14549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEJZ010000073 DACEJZ010000000
VERSION     DACEJZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.076"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW656_002091"
     CDS             4..816
                     /locus_tag="IW656_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0837793.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW656_002092"
     CDS             813..1613
                     /locus_tag="IW656_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0837794.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW656_002093"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW656_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0837795.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW656_002094"
     CDS             3110..4819
                     /locus_tag="IW656_002094"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0837796.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IW656_002095"
     CDS             5031..9389
                     /locus_tag="IW656_002095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0837797.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IW656_002096"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IW656_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0837798.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IW656_002097"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IW656_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0837799.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IW656_002098"
     CDS             11227..11520
                     /locus_tag="IW656_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0837800.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IW656_002099"
     CDS             11517..11828
                     /locus_tag="IW656_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0837801.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IW656_002100"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IW656_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0837802.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IW656_002101"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IW656_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0837803.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DACEJZ010000074        14499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEJZ010000074 DACEJZ010000000
VERSION     DACEJZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.077"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(308..670)
                     /locus_tag="IW656_002102"
     CDS             complement(308..670)
                     /locus_tag="IW656_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837804.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="IW656_002103"
     CDS             complement(744..1124)
                     /locus_tag="IW656_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0837805.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1206..2381
                     /locus_tag="IW656_002104"
     CDS             1206..2381
                     /locus_tag="IW656_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0837806.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2458..3042)
                     /locus_tag="IW656_002105"
     CDS             complement(2458..3042)
                     /locus_tag="IW656_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0837807.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="IW656_002106"
     CDS             3243..4241
                     /locus_tag="IW656_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0837808.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="IW656_002107"
     CDS             4353..4862
                     /locus_tag="IW656_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0837809.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="IW656_002108"
     CDS             complement(4892..5551)
                     /locus_tag="IW656_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0837810.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="IW656_002109"
     CDS             complement(5553..6317)
                     /locus_tag="IW656_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0837811.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="IW656_002110"
     CDS             complement(6325..7002)
                     /locus_tag="IW656_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0837812.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="IW656_002111"
     CDS             complement(7039..8424)
                     /locus_tag="IW656_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0837813.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="IW656_002112"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW656_002112"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="IW656_002113"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="IW656_002113"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0837814.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9363..10220
                     /locus_tag="IW656_002114"
     CDS             9363..10220
                     /locus_tag="IW656_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0837815.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10301..10636)
                     /locus_tag="IW656_002115"
     CDS             complement(10301..10636)
                     /locus_tag="IW656_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837816.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10703..12073)
                     /locus_tag="IW656_002116"
     CDS             complement(10703..12073)
                     /locus_tag="IW656_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0837817.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12151..12687)
                     /locus_tag="IW656_002117"
     CDS             complement(12151..12687)
                     /locus_tag="IW656_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0837818.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12996..14477
                     /locus_tag="IW656_002118"
     CDS             12996..14477
                     /locus_tag="IW656_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0837819.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4469 a         2886 c         2355 g         4789 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca
     9361 agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag
     9421 catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag
     9481 attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc
     9541 aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa
     9601 gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc
     9661 aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag
     9721 atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta
     9781 tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc
     9841 attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag
     9901 agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt
     9961 actatacaaa agatactgta aaagatagta agttgatcaa aagttacata gaactgatca
    10021 aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct
    10081 ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc
    10141 aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat
    10201 tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac
    10261 cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa
    10321 aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag
    10381 agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac
    10441 tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga
    10501 cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg
    10561 atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt
    10621 ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa
    10681 aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta
    10741 ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc
    10801 atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt
    10861 gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc
    10921 taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta
    10981 aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa
    11041 gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct
    11101 cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt
    11161 gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata
    11221 tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg
    11281 attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga
    11341 tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt
    11401 ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag
    11461 attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac
    11521 aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg
    11581 gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa
    11641 tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag
    11701 cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg
    11761 gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc
    11821 cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg
    11881 gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta
    11941 gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg
    12001 atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg
    12061 cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa
    12121 ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc
    12181 caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc
    12241 ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga
    12301 cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag
    12361 cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa
    12421 aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta
    12481 gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt
    12541 gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt
    12601 aattgacttc attccatatt cttccacgta taccatactt ttgaacagca aatattcttt
    12661 actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa
    12721 taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata
    12781 tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg
    12841 aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa
    12901 ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta
    12961 actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag
    13021 agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca
    13081 atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac
    13141 tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa
    13201 ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc
    13261 taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca caaactgctg
    13321 taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga
    13381 caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct gtaaaaaatt
    13441 gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag
    13501 atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga
    13561 acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt
    13621 taactttatt catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa
    13681 tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt
    13741 tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt aattattttt
    13801 tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt ggttttttat
    13861 tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact tacttaactc
    13921 aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat tttcttgtgt
    13981 tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct acactttgct
    14041 ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa ttaagagaaa
    14101 aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa taccttagtc
    14161 aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc catttgcaga
    14221 taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag ttatttcgca
    14281 aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt acagatcagc
    14341 atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac aatacagtgt
    14401 tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt tatgagaaca
    14461 aaacaaagag gatgtgacaa aattcttgtc acatcctct
//
LOCUS       DACEJZ010000075        14449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEJZ010000075 DACEJZ010000000
VERSION     DACEJZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.078"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            215..596
                     /locus_tag="IW656_002119"
                     /pseudo
     CDS             215..596
                     /locus_tag="IW656_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            727..1677
                     /locus_tag="IW656_002120"
     CDS             727..1677
                     /locus_tag="IW656_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0837820.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2195..2650
                     /locus_tag="IW656_002121"
     CDS             2195..2650
                     /locus_tag="IW656_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0837821.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2961..3317
                     /locus_tag="IW656_002122"
                     /pseudo
     CDS             2961..3317
                     /locus_tag="IW656_002122"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3330..3740
                     /locus_tag="IW656_002123"
                     /pseudo
     CDS             3330..3740
                     /locus_tag="IW656_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            3855..4019
                     /locus_tag="IW656_002124"
     CDS             3855..4019
                     /locus_tag="IW656_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837822.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4170..5149)
                     /locus_tag="IW656_002125"
                     /pseudo
     CDS             complement(4170..5149)
                     /locus_tag="IW656_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5154..5921)
                     /locus_tag="IW656_002126"
     CDS             complement(5154..5921)
                     /locus_tag="IW656_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0837823.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6411..6836
                     /gene="lacA"
                     /locus_tag="IW656_002127"
     CDS             6411..6836
                     /gene="lacA"
                     /locus_tag="IW656_002127"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0837824.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6853..7368
                     /gene="lacB"
                     /locus_tag="IW656_002128"
     CDS             6853..7368
                     /gene="lacB"
                     /locus_tag="IW656_002128"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0837825.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7379..8311
                     /gene="lacC"
                     /locus_tag="IW656_002129"
     CDS             7379..8311
                     /gene="lacC"
                     /locus_tag="IW656_002129"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0837826.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8314..9294
                     /gene="lacD"
                     /locus_tag="IW656_002130"
     CDS             8314..9294
                     /gene="lacD"
                     /locus_tag="IW656_002130"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0837827.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9322..9639
                     /locus_tag="IW656_002131"
     CDS             9322..9639
                     /locus_tag="IW656_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0837828.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9639..11345
                     /locus_tag="IW656_002132"
     CDS             9639..11345
                     /locus_tag="IW656_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837829.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11487..12893
                     /gene="lacG"
                     /locus_tag="IW656_002133"
     CDS             11487..12893
                     /gene="lacG"
                     /locus_tag="IW656_002133"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0837830.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12916..13188
                     /locus_tag="IW656_002134"
     CDS             12916..13188
                     /locus_tag="IW656_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ0837831.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13209..14108
                     /locus_tag="IW656_002135"
     CDS             13209..14108
                     /locus_tag="IW656_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0837832.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4717 a         2380 c         2792 g         4560 t
ORIGIN      
        1 gcttttcggt gtctactgaa atcaaggtat taatgggaat aacagcctaa gatatttgga
       61 gttcagagag ggtgcgtttg attttcaaac ttcgcaacag aaccgatgac actatcgttc
      121 ttcatcaacg taagttagat ttgttgaaag agcagaaaaa aggatttcta caaaaaatgt
      181 ttgtttgata ttttaaatag aaaagagaga taaagtgaag tttttaaatt ttttgaacta
      241 taaatattta aactgggtat tatatggtat tacattattt tttattattt attacttttt
      301 taccgatgat gatatttttt taattttagc aatacttttt cttgtttcat ctaattttag
      361 tgataactta attgaagttc ttacagaacc agaaattcgg tctatgacgg atgaagaaca
      421 acagattatt aaagctatga tttcggaaaa taagaaagac gtagtagtta tcaaaaagat
      481 tagagagtat acaaatttag gtgttttaca agccaaaaaa gtatatgaaa aaaaaaaaaa
      541 gggtctataa ttacaaatta gataatacat tagaagctgg agacaagatt gtctagataa
      601 tgtgtgagat aaaatttttg gcaggttatc atacataaga caaaatgttt ttaaaactga
      661 taacgtattt attccacaac aattacgcat ttatttctga gaaaaatata aagcaggagg
      721 attacaatgc tgaaaactta ttccttaaca aaagatacat tattagtttc gtcagaatca
      781 aatatcaaaa attcttctgt tgtttatgtt tatggtgacg aacgagaata tatcgaatct
      841 tctgaacata aatatgactt taaaataggc aatatactaa cgtttgatga tcgagttgca
      901 tatgacacga tgatagataa gaacgatgaa aaaagtttgc tcgtaagttt tttgtttccc
      961 gaggtacatg aaggtgggca tctagatacc ctgacgacat ctgttgtttt cttggtattt
     1021 aaaaataagt tgataatttt tacaaaacaa aaattaaaat tcattcccga actcttatcc
     1081 aatatggttt tacgtgatgt caatcatttc gtggaggaag tacttttaaa gatcatgcaa
     1141 aaagatttct atatgatgct taacgacctt cgtggagtca aggaaaaaat tgatgattta
     1201 gactcagaaa tatcaacgtc cggttcactc aaaccaacgt ttggtgattt attgactctt
     1261 caaaaataca tgatagcttt gtcaacaact tataaagcta accataaagc gcttgatttc
     1321 attaaaaaaa atttcacaac tattgacgat attgatttta gtactaaaaa aataattgat
     1381 gatttatacg atacaagtga tacaatggat agaatcattt taagttacag ccaatatttg
     1441 gacaaattgg aaaatatgat aaacaatatg atttcgtatc agctaaacat gattatgaaa
     1501 accctaaccg agatatcaat tgttttgact attccagcaa ttatttttgg cttttgggga
     1561 atcaatgtcc atgtcccgtt tgaaaagtct tcttatggtg ttttagccgt attgattatc
     1621 tctgctatac taagtctaat ttgctggctt ttgatgcgac gcaaaacata tttgtaaagt
     1681 atttgtgata ccctaatatg atttaaagca gaatttcttt gttgactttt taaaaggctg
     1741 attcctttca ataatttaat agcttcagcg gctgctcaga agtcaataaa ttggactatt
     1801 tttattcagt tacttaggca acatttgaga tacgattgcg attataaaaa ttgatatacc
     1861 aaccaatggc tgcttggaag actgacatat tagcctcgat atcatcgaat aactaaatac
     1921 agaaaaatac aataaatatt atcaaactag ggactatact agaaaacatc agtacattcc
     1981 ttaactcagg gtctataatt agaaaatcgc ccctttgaaa actgattaaa acagccccca
     2041 ttatctagtg actagataat gggggctgtt tttttgttaa gtgatataat aaaccataaa
     2101 agaagtttag aaatacggtc ctggtcatca ataaataaac aatttaatta attatttatt
     2161 taattattta atttcagaaa ggtatacatg aatcatggtg caacaaattg tcctacccat
     2221 caaagactcc agcgtcttaa agatggtaca agatacactt ctcgatagtt tccgtgctgg
     2281 tcgccgaaat tacaccgttt ttcaagttgg taaggccacg ctactacgtg tgagtgatgt
     2341 catgacatta aaaaaatcag atgtctataa tccagacggc tctgtcaaaa atgcaacctt
     2401 tattcatgat aaaaagaccg gtaaagcaaa cacgttatat ttaaagcctg ttcagcaaga
     2461 cttgttgcaa tatcatgatt ggctggccca agaaaatatc aattctgagt ggttatttcc
     2521 ctcgacagcc cattatgatc ttcatatcac tgagaagcag tttcataagg tgatggcgca
     2581 tgttggtgat ctcttaggaa tcaacttgga agacaaagag aaaaaattgc gagtcagaaa
     2641 accgttttga gatatgaaca gcatttgcct tcacgcctta gcgattttaa ataatagcct
     2701 tgaaagtgat tatggaaaag ccctctacgc tcaatgcaaa attgacgtag aaactatttt
     2761 tgatcgattg aaaggtgtat ttggtatgtg aagaacccat gtcagaggca agaacgttgc
     2821 acctactgac attggaatga tgcttatgag catgaatcta accaaattat cccttgaggc
     2881 tagaagaaaa gcggaagctt ttcaacacaa agcagccaaa aataaaaaac gcgacgaagc
     2941 aatcacattt atgattattc atcgcgtttt ttatatttag agctagttat ttgctagacc
     3001 cactatttac agatgaaacg tttatcacct ttggttttgg gcatttagtg gaattagcag
     3061 aaccaggtca ttatgacgaa aagtggcaaa attggaaact tgaatctttg ccgatttttc
     3121 ctgatcgata cgattttgaa gttgcaaaag ataagggaaa gcagtttaaa attgttgcag
     3181 aacttctcaa aaaggcaaat acaattattg ttgcaacaga tagcgacaga gaaggtgaaa
     3241 atatcgcctg gtcgattatc cataatgcaa atgccttttc aaaagataaa acatttaaaa
     3301 gactatggat caatagctta gaaaaagatg tgatccgaag cggttttcaa aatttgcaac
     3361 caggaatgaa ttactatccc ttttatcaag aagcgcaaac acgccaaatt gccgattggt
     3421 tgatcggcat gaacgcaagc cctttgtata cgttaaattt acaacagaag ggcgtacaag
     3481 gtacattttc actaggacgt gttcaaacgc ccaccttata ccttattttt cagcgccagg
     3541 aagccataga gaattttaaa aaagaacctt ttttcgaggt ggaagctaag taaaacaagg
     3601 gtcatttaag ggcgtgctaa gccccacaca gcgttttaaa acccaagagg agctttttag
     3661 cttttgtttc ttctaaacaa gctaaaatag gcaatcaaga ggggataatt gctgatgttc
     3721 aaaccaaaga gaagaaaacg aatagtccga gttcgttttc tttaagtagt ttgcaatcaa
     3781 aagtcaatca gctttataaa gcgacagcga gccaaacttt aaaagctatg caaggactgt
     3841 atgaagcaaa attattgagt tatccaagaa cagatacacc atttattaca gagaacgaat
     3901 ttgcttattt aaaagcgaat tttggcaaat atagcggttt tttaggactt gatcttgaaa
     3961 tgttgttatc attgataatg atacgttcaa acaacagcac ccaaattttg acgaattagt
     4021 gaaactctat gaaaaagacg tagtaaaaca tgcaacatcc tattctaatc agctggtaaa
     4081 gcttaattag gttatataag cttgaaaata aagggtttgt ccaatatgga caagcctttt
     4141 tcttatgttt tgccacagat ttgccacact tattgtgtaa gagtatcaag gataggtata
     4201 atttgatact ttgttttttt agtgatgtgc aaataaattt ctgttacacg actacctcta
     4261 gaatgcccta cacggtcttg aattgcttct aggggtattc cttactctgc taagaaacta
     4321 atatgcgtat gacggaatat atgggtcgta atatgtttat taaaactaag tttattatca
     4381 atatcaattc ctgctcgttt tggattacct tttcgattta attttccatt ttcaggtgag
     4441 ttgaggaaat tgttaaggac actataatga agaggtgttc cattatagtc gggtttatga
     4501 cgacttttgg aagtaaaaat ccatccatta tctttataat caggattgaa tctcatatgt
     4561 tggtgattta actcaatttc ctctttaaga atttctatag cccttttaga taattggaca
     4621 tttcggtaac tttgtaagtt ttttacatta ccttcatagc tattttctgc agctcctccg
     4681 tgagtggact gactaatgat gatattttgc ttttcaaaat attgataggc accaatattt
     4741 tggagtttcg actctgtaag tcaacagggc cattagttag aatacctatt gggatgcggt
     4801 ttttatgtag agccagcagt agttgatgta tctcaggaat catcgtaatc tgatcgagtt
     4861 catattcata gtcagcttgg aagtctagag cttctgtcct agaaatacta tcgatgccaa
     4921 attcttccat agtcttctga cagcgaaaaa ttctaagctg ctcaatactg atgagttttt
     4981 tcgtgtattt aggaaaagca acatcagacc aatatctgaa acgtttgtaa atgttatcta
     5041 tttgttggat atcaatcgtt ggaaaacatt tctcaacgct acgtttaaag gggagttgat
     5101 ggtcataaag agtgtcatca aggtcaaaaa caatattttt ataagtcata attctagtcc
     5161 gcttttaaaa tagtagtgta ctggctaaat tcctctaagt gttgagggct aatctggtta
     5221 tctgtaatga tggtatcgag ttgatctaga ttatagaagt taaagaaatc gtatcgatcg
     5281 aatttagtac tgtctactaa taagaatttt tctacagcat tgtttagggc aagttgttga
     5341 atcacacctt ccttgtcact atatgtagca atagaattat gggtaacagc atttgcacta
     5401 acaaaagctt tggcaaatct aattgctttt aaatttgtcg aagccattga acctacaaaa
     5461 gctccagtta tttctctata ttcaccgcca agaagcaata aatcaatggt ttcgctatca
     5521 tttagaatca agaacacagg gagactattt gtaatgacac gaattttata acctttacga
     5581 cctttcaatt ctaatgccag ttgttcaagt gtagttccag gtccaataaa aatagtatcg
     5641 ccatctttga ttaaagagca ggctttttta gcaatgtcaa ttttttcttt tgtatttaga
     5701 cttttcttct cgatatgtgt cttttcaagt ggctttttag aggaaagcaa ttgtgcacca
     5761 ccatgagtac gtgttaaaag tccatcagct tctagagcat caaggtctct acgggctgtc
     5821 atatcggaaa tatctagttc atctattatt tcttttatgg ttatggtccc atcaatcttt
     5881 aacatatcta aaattttttc taatcgtcgt tttttgttca tatgaagacc cttctttcat
     5941 aaagtaattt ttttccaaag ataattctct tttaattgta tcataaaaga caatattttc
     6001 aaggtaaaac aaacaatttc aaacaaaaac aaatgttaga tgatgaaata agaacagagg
     6061 attgacgtat attagcttag gtcagatttt gtataagacg aaaataaagt aggacctctt
     6121 aatcagtaag ttatagaaag taaaagactt ttgtaatact tgaatagata tttcacgtcc
     6181 attttgtgat ggattaaatg aacaaaaatg aacaataatt taacggtgtt atctattttt
     6241 taaaaaaaca aataaaaaaa acaaaaaata aacaaaaaaa gtgttgcttt ttgtttgaat
     6301 gcttgatatt atataaacaa agaaatgatg aaaacgtttt ttcgaacatt ttgcaaaatg
     6361 ttttctactt ctacgtagca tttctttcta aaatttagga ggtggtcaaa atggctattg
     6421 ttgttggtgc agatctcaaa ggtacgagat taaaagatgt tgtgaaaaat tttcttgtag
     6481 aagaaggttt tgaggtaatt gatgtgacta aggacggaca agattttgtt gatgttacct
     6541 tggcagttgc ctctgaagta aataaagatg agcaaaactt aggtattgta attgatgctt
     6601 atggagctgg tccattcatg gtagcaacta aaattaaagg tatggttgca gcagaagttt
     6661 cagatgaacg ctcagcttat atgacacgtg gacataacaa ttcacgaatg attacggttg
     6721 gcgctgaaat cgtcggtgat gagcttgcta aaaacatcgc taaaggtttc gtcaatggta
     6781 aatatgatgg cggacgtcat caaatccgag tagatatgct taacaaaatg tgttaagaaa
     6841 aggagttaag gaatgagaat cgcaattgga tgtgaccaca ttgtcacaga tgaaaaaatg
     6901 gcagtatcag aattcttgaa atcaaaagga catgaggtcc ttgatttcgg gacatatgac
     6961 cacgcacgta ctcactaccc tatctatggt aaaaaagtcg gtgaagcagt agtaagtggt
     7021 caagcagact tgggagtatg tatctgtggt acaggtgtcg gcattaacaa tgcagtcaat
     7081 aaagttccag gtgttcgctc agcgttggtt cgtgatatga catcagctat atatgcaaaa
     7141 gaagagttaa atgcgaatgt tatcggtttc ggtggaaaga ttactggtgg tcttcttatg
     7201 aatgacatca ttgaagcttt cattgaagct gagtacaaac caacagaaga aaataaaaaa
     7261 ttgattgcaa aaatcgaaca cgttgaaaca cataatgcac atcaagcaga tgaagatttc
     7321 tttacagaat tccttgaaag atgggaccgt ggtgactacc acgattaagg tataacaaat
     7381 gattctgaca gtcacactaa atccttcagt agacatctct tatcctctag aaacattgaa
     7441 aattgataca gtgaataggg tgaaagatgt tagtaagaca gctggtggaa aaggcttaaa
     7501 tgttactaga gtcttgtatg aatctggtga taaagttact gctacaggtt tcttaggtgg
     7561 taaaattggt gaatttattg aaagcgaatt agaacaatct cctgttagtc cagcttttta
     7621 taaaatctca ggaaacacta gaaactgtat tgctatctta cacgagggta accaaacaga
     7681 aattttggaa caaggaccaa caatttctca tgaagaagca gaaggctttc ttgaccacta
     7741 tagcaatctt attaaacaat cagaagttgt gacaatttca ggaagtttac cttcaggact
     7801 tcccaacgat tattatgaaa aacttatcca acttgcttcg gatgaaggag tagcagtcgt
     7861 tttggattgt tcaggtgcac ctcttgaaac agttctaaaa tcgtcggcta aacctactgc
     7921 catcaaacct aataatgagg aactttcaca gttgcttgga aaagaagtaa caaaagatat
     7981 tgaagaactc aaagacgttc ttaaagagtc actttttagt ggtatcgaat ggatagtggt
     8041 ctcattaggt cgaaacggtg cctttgcaaa acatggtgat gttttctata aggtagatat
     8101 tcctgatatc ccagttgtta atccagtcgg atcaggggac tcaacggtag ctggtattgc
     8161 atcagcttta aatagtaaaa aaagtgacgc tgacttatta aaacatgcga tgacattggg
     8221 tatgttaaat gctcaagaaa caatgacagg gcatgtgaat atgactaatt acgaaacact
     8281 aaactcacaa attggagtaa aagaggtata aaaatggtac ttacagaaca gaaacgcaaa
     8341 tctttggaaa aaatttcaga taaaaacggt gttatctcag ctttggcatt tgaccaacgt
     8401 ggtgctttga aacgtttgat ggcacagtat caagatacag aaccaactgt agctcaaatg
     8461 gaagaactaa aggttttggt tgctgatgaa cttacaaaat acgcttcttc aatgttgctt
     8521 gaccctgaat atggacttcc agcaacaaaa gcattggata aagaagcagg tcttcttctt
     8581 gcctatgaaa aaacaggtta tgatacatca agtacaaaac gtttgcctga ctgcttggat
     8641 gtttggtctg caaaacgtat caaagagcaa ggcgctgatg ccgttaagtt cttgctttac
     8701 tatgatgtag acagctcaga tgaacttaac caacaaaaac aagcttatat tgaacgtgtt
     8761 ggttctgaat gtgtcgctga agatattcct ttcttcttgg aaatccttgc ttatgatgaa
     8821 gaaatctcag atgcaggttc tgttgaatat gcaaaagtga aacctcgcaa agtcattgaa
     8881 gcgatgaaag ttttctctga tccacgcttt aacattgatg ttcttaaagt agaagtacca
     8941 gttaacgtta aatatgtcga aggttttgct gatggtgaag tagtttacag taaagctgaa
     9001 gctgctgact tctttaaagc acaagaagaa gcgactaacc ttccatacat ctacctaagt
     9061 gcaggtgtat ctgctaaatt gttccaagaa acattgcaat ttgctcacga ctcaggtgcc
     9121 aagtttaatg gtgtgctttg tggacgtgct acttgggcag gatctgtaga accttacatc
     9181 aaagaaggtg aaaaagcagc acgtgaatgg ttgcgtacta ctggattcga aaatattgat
     9241 gaacttaaca aagttcttgt taaaacagct agtccatgga ctgataaagt atagtaaaaa
     9301 cataaaacgg aggatattgt tatgaacaga gaagagatga ctctcttagg gtttgaaatt
     9361 gttgcttatg ctggagatgc tcgctctaaa cttttagaag cgcttaaagc ggctgaaaat
     9421 ggtgatttcg ctaaggcaga tagtcttgta gtagaagcag gaagctgtat tgcagaggct
     9481 cacaattctc agacagctat gttggctcga gaagcttctg gggaggaact tccatacagc
     9541 gttaccatga tgcatggtca ggaccactta atgactactg tcttattaaa agatgtgatt
     9601 catcacctca tcgaacttta taaaagagga gcaaaataat gcataaactc attgaactta
     9661 ttgagaaagg gaaaccgttc tttgagaaaa tctctcgaaa catctatctt cgtgctattc
     9721 gtgatggatt tatcgctggt atgccagtca tccttttctc atctatcttt atccttattg
     9781 cctatgtccc aaatgcttgg ggattccact ggtcaaaaga cattgagacc ttcttgatga
     9841 ctccttatag ctattcgatg ggtatcctag cattctttgt tggtggtact accgctaaag
     9901 ctttgacgga ctcaatgaac cgtgacctac ctgcgaccaa tcagattaac ttcttatcta
     9961 cgatgctagc gtctatggta gggttccttt tgatggcagc tgagcctgca aaagaaggag
    10021 gcttcttgac agccttcacg ggaacaaaag gtcttttgac agcatttatt gcagcctttg
    10081 tgacagttaa tgtttacaag gtctgtgtga aaaataatgt tactattcgt atgcctgaag
    10141 aggttccacc aaatatctct caagtattta aagacttgat tccattcact gtagcggttg
    10201 ttctccttta tggatttgaa ctcattgtta agggaactct tggtgtaacc gttgcagaat
    10261 caatcggtac ccttcttgct cctctcttct cagctgcaga tggttacctt ggaattacac
    10321 ttatctttgg tgcttatgcc ttcttctggt ttgttggtat ccacggtcct tcaattgtcg
    10381 aaccagcaat cgctgctatc acttatgcca atatcgatgc caacttgcat cttatccaag
    10441 ctggacaaca tgcagacaaa gttatcactt ctggtactca aatgtttatt gttaccatgg
    10501 gtggaacagg agctacattg attgttccat tcttgttcat gtggatttgt aaatcagaac
    10561 gtaaccgtgc catcggacgt gcctcagttg ttccaacatt ctttggggtt aatgagccaa
    10621 tcttatttgg tgcaccaatc gtattgaatc cgattttctt tgtaccgttc attttcgctc
    10681 cgattgtcaa cgtttggatc tttaaattct ttgttgacac attgaacatg aactcattct
    10741 ctgccaacct tccatgggtt actcctggtc cgttgggaat tgtactcggt actaacttcc
    10801 aagttctatc atttatctta gccggactct tggtagttgt tgatactatc atttattacc
    10861 catttgttaa ggtatacgat gaacaaattc ttgaagaaga acgttctggt aaaacaaacg
    10921 atgctcttaa agaaaaagta gctgcaaact tcaatactgc taaagcagat gccgttcttg
    10981 gaaaagcagg cgttgctaaa gaagatgtag cagcaaacaa taatatcaca aaagaaacta
    11041 atgtccttgt tctttgtgca ggtggaggta caagtggctt gcttgccaat gccttgaaca
    11101 aagcagcagc agaatacaat gttccagtca aagcggcagc gggtagttac ggtgctcacc
    11161 gtgaaatgtt gccagagttt gacttggtta tcttggctcc acaggtcgct tcaaacttcg
    11221 atgatatgaa ggctgaaacg gataaattgg gcattaaact tgcaaaaact gaaggagctc
    11281 aatatatcaa attgacacgt gatggacaag gtgctcttgc atttgttcaa caacagtttg
    11341 attaataaat ttgtaaaagt tatttgagac gagattattc tttcaattcc ttataatatt
    11401 gttaaccata tcattgcatt tcatttcctt ggtttccgga actcagaata tcgtctcaaa
    11461 tgcttttttt gaaaggattt atagttatga ctaaaacact tcctaaagat tttatttttg
    11521 gtggtgctac agctgcttat caagcagaag gtgcgactca cacggatggt aaaggcccag
    11581 ttgcatggga taagtatctt gaagacaatt attggtacac agcagagcct gcaagtgatt
    11641 tttaccataa atatcctgtt gatttagaat tagctgaaaa atatggcgtt aatggtattc
    11701 gtatttcaat tgcctggtct cgtattttcc caactggcta tggcgaagta aatgaaaaag
    11761 gtgttgaatt ctaccataat ctttttgccg agtgccacaa acgtcatgtt gagccattcg
    11821 tgactttgca ccactttgac acaccagaag ttcttcactc agatggagat ttcttaaacc
    11881 gcgaaaatat agaacacttt gtagattatg ccgctttctg ttttgaagag ttcccagaag
    11941 taaactactg gacaactttc aatgaaattg gcccaattgg agatggtcaa tacttggttg
    12001 gtaaattccc tccaggcatt caatatgatc ttgccaaagt cttccaatca caccacaata
    12061 tgatggtttc tcatgcacgt gctgtgaaat tgtataaaga taaaggttat aaaggcgaaa
    12121 ttggtgtagt acatgcgttg ccaaccaaat acccttatga tccagaaaat ccagcagatg
    12181 ttcgtgccgc tgagttagaa gatatcatcc ataacaaatt tatcttggat gcaacctacc
    12241 ttggacacta ttcagataaa acgatggaag gggtcaacca catcctagct gaaaatggtg
    12301 gagaacttga tcttcgtgat gaagacttcc aagcacttga agcagctaaa gatttgaacg
    12361 atttccttgg tatcaactac tacatgagtg attggatgca agcctttgat ggtgagactg
    12421 aaatcattca caatggtaag ggtgaaaaag gaagttctaa gtaccagatt aaaggtgttg
    12481 gtcgccgaat cgcaccagat tatgtgccta gaacggattg ggactggatt atttatcctg
    12541 aaggcttgta tgaccaaatc atgcgagtga aaaatgatta tccaaactac aagaagattt
    12601 acatcactga aaacggtctt ggatacaaag atgagtttgt agataatacc gtttatgatg
    12661 atggtcgtat tgattacgtg aagaaacact tggaagtttt atcagacgca attgcggatg
    12721 gtgcaaatgt taaaggttac ttcatctggt cacttatgga tgtcttctca tggtcaaacg
    12781 gatacgaaaa acgttatgga ctattctatg tagactttga cacgcaagaa cgctatccta
    12841 agaaatcagc tcattggtat aagaaagtag cagaaactca agtgatagag taatattact
    12901 tgaaaatgta aaaaattgtt atccgatact ttagaaaaag tacaacactt atttagacca
    12961 ggctttgaaa aggtatatat tgttagtttc gaagattgtc ctatgattcc tgagcttgaa
    13021 gctaccccgt tgctaaaatg tggtaaatgg tatgtttcaa ctggaaaaga gtggatttgc
    13081 cattctgatt tagagttatc agcatttgaa tgggaatttt tacaatcact cgatgtagaa
    13141 atacgtgaga ctattcactt cgaggtaaat tatttgcctt tccaataata aaaagaaagg
    13201 aattagttgt gactatcgaa ttaaaaaacg agtatcttac tgttcagttt aaaacacttg
    13261 gtggtcaact gacttcgatt aaagacaagg atgggataga gtatctctgg caagctgatc
    13321 caaattattg gaatggacag gcgcctattc tatttcctat ctgtggtagt ttacgaaatg
    13381 attgggctat ttatagacct caagaaagac ccttttttac aggtcttatt cgtagacatg
    13441 gttttgttcg aaaagaagaa tttattctgg aagaagtcaa tgataatagc gtgaccttta
    13501 gtattaaacc aaatgctgag atgcttgata attacctata tcagtttgaa ctgagagttg
    13561 tttatacctt gaatggtaaa tcaattagaa ccgaatttca ggtaacgaac ttggaaatgg
    13621 aaaaaacaat gccttacttt attggagccc atccagcatt caatagtcct ttagtagaag
    13681 gtgaaaacta tgaggattat tatattgaat ttagtgaggt tgagtcttgc tcgataccaa
    13741 agagttttcc tgagacagga ttattagatt tgcaagatcg gacacctttt ttggaaaatc
    13801 aaaaaattct cgatttggac tactcacttt tttcccatga tgccattaca cttgaccagc
    13861 tcaagtctcg tagcgtaacg cttcggtcaa ggaagtctgg taaaggtctt cgagtagatt
    13921 ttgatgattt tccgaacttg attctgtggt ctacaactaa taaaagtcca ttcattgctt
    13981 tggaaccttg gagtggtctc tccacttcac ttgaagaagg taatttctta gaagataaaa
    14041 gacaggtaac gaaaatttca cctcaagaaa cttctagaaa aatctatgat attactatcc
    14101 ttaattaaaa tttgttatta tttgagtctt caataagatt tgaatactct taccttgtga
    14161 tgatagattg gtcttctccg tgccagtctt acatcgcaag gtttttaagt tttttgtggg
    14221 tctatgtcaa ctgtagtggg tgacgaaaag ctaatatcta aagacagtct ggtagtaaaa
    14281 aataagatta tttactactt cttgatttaa aataatagtg gagccattaa tttgtggcaa
    14341 atttaattta atattttgcc tcaaattagc cacacttttt atagaactaa gaggaaaaat
    14401 gaaattttct tcctattaat atagttgttt gaaatcttaa atatagcgt
//
LOCUS       DACEJZ010000076        14374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEJZ010000076 DACEJZ010000000
VERSION     DACEJZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.079"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..407
                     /locus_tag="IW656_002136"
                     /pseudo
     CDS             1..407
                     /locus_tag="IW656_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(309..1025)
                     /locus_tag="IW656_002137"
     CDS             complement(309..1025)
                     /locus_tag="IW656_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ0837833.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1149..1334)
                     /locus_tag="IW656_002138"
     CDS             complement(1149..1334)
                     /locus_tag="IW656_002138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837834.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1561..2834
                     /locus_tag="IW656_002139"
                     /pseudo
     CDS             1561..2834
                     /locus_tag="IW656_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2941..3972)
                     /locus_tag="IW656_002140"
     CDS             complement(2941..3972)
                     /locus_tag="IW656_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837835.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3979..4809)
                     /locus_tag="IW656_002141"
     CDS             complement(3979..4809)
                     /locus_tag="IW656_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837836.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4806..5612)
                     /locus_tag="IW656_002142"
     CDS             complement(4806..5612)
                     /locus_tag="IW656_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0837837.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5618..6442)
                     /locus_tag="IW656_002143"
     CDS             complement(5618..6442)
                     /locus_tag="IW656_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ0837838.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6432..7532)
                     /locus_tag="IW656_002144"
     CDS             complement(6432..7532)
                     /locus_tag="IW656_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0837839.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7710..8495
                     /locus_tag="IW656_002145"
     CDS             7710..8495
                     /locus_tag="IW656_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ0837840.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8528..8911
                     /locus_tag="IW656_002146"
     CDS             8528..8911
                     /locus_tag="IW656_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0837841.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8987..9118
                     /locus_tag="IW656_002147"
     CDS             8987..9118
                     /locus_tag="IW656_002147"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0837842.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9087..9323
                     /locus_tag="IW656_002148"
     CDS             9087..9323
                     /locus_tag="IW656_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0837843.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9401..10372)
                     /locus_tag="IW656_002149"
     CDS             complement(9401..10372)
                     /locus_tag="IW656_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0837844.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(11214..14363)
                     /locus_tag="IW656_002150"
     CDS             complement(11214..14363)
                     /locus_tag="IW656_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0837845.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         4177 a         3269 c         2667 g         4261 t
ORIGIN      
        1 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
       61 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      121 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      241 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      301 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      361 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      421 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      481 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      541 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      601 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      661 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      721 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      781 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
      841 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
      901 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
      961 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1021 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1081 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1141 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1201 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1261 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1321 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1381 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1441 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1501 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1561 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1621 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1681 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1741 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1801 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     1861 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     1921 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     1981 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2041 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2101 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2161 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2221 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2281 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2341 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2401 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2461 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2521 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2581 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2641 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2701 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2761 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     2821 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     2881 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     2941 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3001 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3061 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3121 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3181 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3241 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3301 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3361 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3421 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3481 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3541 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3601 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3661 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3721 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3781 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     3841 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     3901 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     3961 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4021 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4081 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4141 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4201 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4261 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4321 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4381 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4441 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4501 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4561 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4621 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4681 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4741 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4801 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     4861 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     4921 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     4981 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5041 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5101 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5161 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5221 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5281 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5341 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5401 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5461 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5521 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5581 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5641 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5701 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5761 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     5821 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     5881 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     5941 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6001 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6061 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6121 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6181 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6241 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6301 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6361 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6421 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6481 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6541 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6601 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6661 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6721 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6781 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     6841 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     6901 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     6961 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7021 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7081 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7141 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7201 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7261 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7321 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7381 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7441 tcacctgaga cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7501 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7561 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7621 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7681 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7741 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7801 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     7861 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     7921 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     7981 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8041 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8101 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8161 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8221 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8281 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8341 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8401 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8461 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8521 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8581 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8641 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8701 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8761 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     8821 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     8881 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     8941 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9001 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9061 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9121 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9181 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9241 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9301 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9361 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9421 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9481 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9541 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9601 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9661 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9721 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9781 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
     9841 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
     9901 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
     9961 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10021 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10081 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10141 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10201 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10261 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10321 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10381 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10441 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10501 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10561 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10621 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10681 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10741 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatgattcc
    10801 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
    10861 ataaactatt ttgggttaca tggtatctga aacagctaaa accttgtcat tacagcaaaa
    10921 aatcacttta tctccgtttc atttcagata catttcactt aagcggattc caaaaaaaca
    10981 aaagaaaaag gaacccctag ttagcctatg ctttttattg gttcctctca aaacacggtc
    11041 atataacaac ggggtggggt aaatgaatga aaaaggtgga ggaaacatta ttcaatagct
    11101 gtcttaaata cttcgttata aaatgtgaac aaataaaaga catctatccc tattttctac
    11161 acttccactt tactaccatc tttgaatcgt acaattttct taactcttct cgtttacctc
    11221 aattcatttt caagtggaat cagctcatca tctaacattt tacgccatgc tcgcttcatt
    11281 tcttgtgggt atttaaatgg cttcgcttct ggatggttgg ctttatatcc ttcgtaatcc
    11341 ttactgttga taattgcttt catattagga attggatctt cacctggcat atattgcaga
    11401 ttagaagcat gtaattcttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa
    11461 tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attactatct
    11521 aaagtcccca catcaaacgc atttaaaatt tgtcggtaaa cttctttgac tccttctgtt
    11581 ttattcaatt tagccaaagc tttttcaatt tcttctcgtg catctgaact attcgccgca
    11641 taagttccta gtaattgatc aatataagta gaatcaatat catacaactt cgtggtattt
    11701 tcactataaa attcgatcgt ggaaaaatct tctccctctt tttctggttc ttcctctatt
    11761 aatgaaccct tcacggtctc gtacacgcca atttgttctt ccaaaagttg aatttgattg
    11821 ttcaaggttg aagatttcac catgtcatca ttgtaatcgt cataagtcac taacgattca
    11881 taagcacgat tcaattcttg gaatgctttg acgtactcga tacggatttc aagtggcgta
    11941 tgttcatcaa tggattccat ggatgaaacc tcttttaact taccatatgc ttgcttgaat
    12001 ctttttttgg attcctgata acttggataa atcaaacccg attcttcttt tgcttctgaa
    12061 tacagttttg tcgcatcctc gacattcttc tccattgtgg ctggctttcg gaaagtaaca
    12121 attagtcctt ttgttttttc tggatacgta cggttcgtac gtgaaaaagc ttggatcaat
    12181 cctgcatact ctagattacg atccacaaat agggtttgaa tcgtaggtgc gtcaaaacct
    12241 gtcaatagac gatccactac gatcactaaa tcaatttgtt tcccaaattg tttgaattcc
    12301 gctctttttc gtgccaaacg attattgatg tctccgttat aacgctcaat gtctgctaat
    12361 gaccaggaag tatcataata ctgattatat tcctcaataa ttttttgcat ttcttcttgt
    12421 tggacacttg aatctcggct gttttcttct aatgaatagg taatagcgat tcgtggaaaa
    12481 tcaggatcct ccatcgttct tccttcacga atgggctgat taggaaactc ttttgttaac
    12541 cagtcaggtt ctttcgtcat ttctttgatc gctcgataat actttttggc catgtcaatg
    12601 gaactcgtgg ttaagatcgc agacttcgtt ggacggccat ttcgaaaatc aaattttgta
    12661 taggcattgt ctggacggaa cattttatga atgacttttt ggatatgttc atctgcttca
    12721 aaaacagtag ggtccaaata actttctttt ttgacaggct ccatttgatc aatcatacga
    12781 ttgatctgtt ccggtgaata ctctgcatat gtttctactt cttttaattt ccgatagatt
    12841 ttattctcta atgaagtcgg ctccacggtg ttttcatgct ctacttgaaa tcctaaaacc
    12901 gagccatctt ctaatgcatt cttgatggta taggtatgca agacctctcc atactgatca
    12961 tgggtcgtac gtgccaattg ccctttggct tgtttccgat tctcagggaa aatcggtgta
    13021 cctgtaaagc caaaccaagt ggatttcgga aacatttttt tgatttcttc catattttca
    13081 gcacttaacg ctcgatgaca ctcatctact acaaacacga tatgttgccc cattaatttt
    13141 tggaagcgat tggttccttt tttctcttct tgtttttttg catatttcaa cgccgcatct
    13201 agcttttgac gggttgtaat aatcacaaca tttgcattgg catctgccaa cagagtttca
    13261 cttaactctt tggcacttcc tgttcccacg atcaagctat tcgctttggc gttcccggaa
    13321 gaaatcccag tattaaattc cgaagcaaat ttcgtaaact ctgtggtggt ttgattatct
    13381 aaatctttgc ggtccactaa catgatcgtc cgatcgatcc ccgattttcg tgctaaaagc
    13441 ttagtagata caaaactagt caacgttttt cctgaaccag tcgcatgcca cacatatcct
    13501 gattgatgtt tatttgcagc aataaacaag gcttgaatcg catgtacttg ataaggatgt
    13561 aacaccatga ggattttatt gtcttgatct tcactgacaa tcgtataatc cgcaatcaag
    13621 cgatgagcat ccgggatatt tagcacttgt ttgcaaaact catataagtt ttctaccttt
    13681 ttattatctt tcgttcgcca actaaataaa aacttaggat gcaaatcctt tggtaaggca
    13741 tttgcaaaat accgagtggt ttgttcattg gaaaccacaa acaactgtaa ggtcgaaaag
    13801 atattgttcc gaaacatacc ttcttctgca tattttttga tttggttaaa cgcctgataa
    13861 aaactatctt ttgcggttac ttgtttcagc tcgatttgga caatcggtaa tccattgatc
    13921 agtaaggtga cgtcaaaacg tcgatcccgt gcttctatgt tacttccacg ttttgcaatt
    13981 tgatggacca cttcataggt agagattcct ccaccaatgt cttgattaga ataaagaatg
    14041 agcgagacag aacccagttg cggatcttcc cgttcaatcg tgatccgagc cattccattt
    14101 tcacctttta accatttcgc agcatcaaag ggggttttcg ttcgtagtaa caattctgtt
    14161 ttgatcgttt caaattcttt atcggtcaaa ggaaaatcat tcagttctgc ttgattattt
    14221 gagatgattt tttggcgtaa attgacccaa agatcttctt ctgattttag atcgggacga
    14281 tacgtccact gattatgccc ttcccccaat acacggatca actgttcttc gacttccgct
    14341 tcctcacggt gactaatttt catttatctt ccct
//
LOCUS       DACEJZ010000077        14255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEJZ010000077 DACEJZ010000000
VERSION     DACEJZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.080"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            214..1500
                     /gene="efmA"
                     /locus_tag="IW656_002151"
     CDS             214..1500
                     /gene="efmA"
                     /locus_tag="IW656_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0837846.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1638..2237)
                     /locus_tag="IW656_002152"
     CDS             complement(1638..2237)
                     /locus_tag="IW656_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0837847.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2641..3762
                     /gene="mnmA"
                     /locus_tag="IW656_002153"
     CDS             2641..3762
                     /gene="mnmA"
                     /locus_tag="IW656_002153"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0837848.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            4008..5429
                     /gene="arcD"
                     /locus_tag="IW656_002154"
     CDS             4008..5429
                     /gene="arcD"
                     /locus_tag="IW656_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0837849.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5443..6561
                     /locus_tag="IW656_002155"
     CDS             5443..6561
                     /locus_tag="IW656_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0837850.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6621..7913)
                     /locus_tag="IW656_002156"
     CDS             complement(6621..7913)
                     /locus_tag="IW656_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0837851.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8063..8623
                     /locus_tag="IW656_002157"
     CDS             8063..8623
                     /locus_tag="IW656_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0837852.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8752..9129
                     /locus_tag="IW656_002158"
     CDS             8752..9129
                     /locus_tag="IW656_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0837853.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9062..11134
                     /locus_tag="IW656_002159"
     CDS             9062..11134
                     /locus_tag="IW656_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0837854.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11345..12292)
                     /locus_tag="IW656_002160"
     CDS             complement(11345..12292)
                     /locus_tag="IW656_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0837855.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12538..13002
                     /locus_tag="IW656_002161"
     CDS             12538..13002
                     /locus_tag="IW656_002161"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0837856.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(13064..14107)
                     /locus_tag="IW656_002162"
     CDS             complement(13064..14107)
                     /locus_tag="IW656_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0837857.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         4617 a         2602 c         3003 g         4033 t
ORIGIN      
        1 ttgtaaggac gacgcaaatt agttgtcttt tttattttta aatttaacac atgttgttat
       61 ttttaatatt ttgctgattt attgtttcta agtaaagaaa tttaaattga aacgattgtt
      121 atttagggaa aaaggtattt tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg
      181 ctatacaaaa tgaaaaagaa aagagggatg tttatggaaa atgaacagtc ggtcgtttta
      241 acgaattgga agagaaatta tttgttcttt ttatcgggac agtttttgtc aggaatcact
      301 agtatggtcg tccaatatgc gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca
      361 attttgagtt ttgctacttt actaggaatg attcccatgg ttttactaag cccatttgta
      421 ggaccgctag tggatcgctg ggataaaaaa gctcttttga ttgtcacgga tattattgta
      481 gcgattttcg cgcttatttt agcagtagtc ggaacgattt cagaatcctt cccgatttgg
      541 ctagtttttg tgtctttgtt tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc
      601 cagtcgatta tgccgacaat cgttccatct tctcacataa caaggacgaa tggacagtta
      661 ggaatggtcc aatcagcgaa tttcatcatt gcgccagctc taggtgcggc tttgttttca
      721 gtcgttcctg tcaattacct tattttatta gatgtattgg gggcagtttt tggtgtcggc
      781 ttattgattt ttgtgaagat cccaaaagtt tctcctgaaa tattggaagt tccgcttact
      841 atttttaaag atgcaaaatt tgggctgcag caattgatgg ataataaagg gttatggtac
      901 ataacgatca atggtgcatt tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca
      961 ttgatgacgc tagattactt tggtggttcc gttggacagg cgggagcagt ggaagtagtc
     1021 tacgcagtcg gaatgctctt aggcggtgca ctgatcagtt ttataggaac atggaaagac
     1081 cgaatgaaac ctattattat agcctacatc attatgggcc tgacgatcgg tgcaagcgga
     1141 ttggttccaa acgatagtca agggttcttg tactttctta tcttgaatgc aggagcaggt
     1201 tgtgcaacgc catatttcaa tacactgctg atggcaatga tccagcagag ttatgaatcg
     1261 aatgttttag gtcgtgtctt agggaacttc aactctttga tgaacctggc aggtccaatt
     1321 ggattattat ttgcaggacc attagctgat cgtttagggg tagaaaaaat gttcttgttt
     1381 tccggaatcg ggattttatt atgcggaatc gttttatttt taacgtcagc tgcccgaaat
     1441 tatgataaag aattacagaa aaaactggta aaagaacacc atgagcaaaa agacgaatag
     1501 tatggaatgg agttgtgaca gaagtcttgt tacaactcca tttctgatcc taatgttcct
     1561 gtggcaataa gcctactcaa atgaaaaata tgagtaatta tctttatttg tttaatactt
     1621 gatatagggc agttttttca cgacctatcg tacttcctct ttgcttcata gatttctttg
     1681 atttgttgct cagataacgg agtgccaaat ggtagttttt cctttaagag ttctctttcc
     1741 ggtgcgtcta cgcctacatt gacatcgttc tcgataggta cgctgtaatg atggatatgt
     1801 ccatgaagat tgcggatagt gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa
     1861 tattgttgat ggtcgaattt aacgatggca cctacatcat aaaaataaaa tttgggactt
     1921 ccgtcagtaa gcctaggatc atttttttct aaataacgga aaaaagcgcg actgtcatgg
     1981 ttccctttga tcaagtggat tgttccttgc agttgagtga ccagttccaa aatttctgca
     2041 tttgcttttt catattgcgg atgcatagca aggtcgccta gatgataaac atggtcggtt
     2101 tcttttatta ctgcattcca gctagtgatc aactgttggt tcatttcatc cacatttgca
     2161 aagagccgcg gagcaaagtc gttttttcct aataagcgtt catggaaaaa gtgcatatca
     2221 ctgataaagt aattcatgta tcttttgcct cccttttttc ttagagtaaa caaaatcact
     2281 tatctgagca actcataaca tctaaaaaga aaaggttgta aatgagacgt gcagctttaa
     2341 gcgataagaa gcaattttga aaaatagttt ttcacaattt ataaaattat tccttattgc
     2401 cgaaaggctg gcccttttta tgagtatgcc aattttttat atcgaatgct caaaagatga
     2461 gtgatttttt ggttgactat catgtttgtg tactgaatag gtccagtaga acagtatttt
     2521 tacgacttga taattaaggg atgaaatttt cagaaatatg acgtataatg taaaatactg
     2581 aaaagttgcg accttcaact cgtagatgtt tcagaatcta attgaaatga tggtgatatc
     2641 atgacagaca acagcaaaac acgcgttgtc gtagggatga gcggcggtgt cgactcatct
     2701 gtaacagcac ttttgttaaa ggaacaagga tacgacgtga tcggaatatt catgaaaaat
     2761 tgggatgaca cggacgaaaa cggtgtatgc acggccacgg aagattataa ggatgtagcg
     2821 aaagtagctg ctcaaatcgg gattccttat tactctgtga attttgaaaa agaatattgg
     2881 gatcgtgtat ttgaatattt cctagccgaa taccgcgcag gacgtacacc taacccagac
     2941 gtgatgtgca acaaagaaat caaattcaaa gcattcttgg attatgcaat ggatcttggc
     3001 gccgattatg tagctactgg acattatgcg caagtcactc gcgatgaaaa cggcgtggtc
     3061 cacatgcttc gtggagtaga caataataaa gatcaaactt acttccttag ccaattatca
     3121 caagaacagc tttcaaaaac gatgttccct ctaggcggca tggaaaaatc agaagtacgg
     3181 gcaattgctg aacgcgcagg ccttgcgact gctaagaaaa aagattcaac gggaatctgc
     3241 tttattggtg aaaagaactt caaacaattt ttgagcaact atctgccagc aaaaaaagga
     3301 aatatggtca ctcttgatgg tgaagtcaaa ggacaacatg ctggcttgat gtattatacg
     3361 attggtcaac gtcaaggact agggatcggt ggcggaggag attcgcaaga accctggttt
     3421 gttgtaggaa aagatctagc gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct
     3481 gcattgtatg ccacaagctt ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg
     3541 ccaaaagaat tcaagtgtac agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca
     3601 gttcgcttat tggatgataa tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc
     3661 actcctggac aagcagttgt attttatgat ggaatggaat gtctgggtgg tggtttgatc
     3721 gaccacgcat atcaagaaac aaaagtttta caatacgttt aagcgatatt aaatgaatca
     3781 aaacagctat ggctaacaaa agaaacacct tttcttttgg atagcatagc tgtttttttg
     3841 caataaaatt aatttttatt cattttatac ttgaaatact tgtgtaaaaa gttcataata
     3901 tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa atagaaggga
     3961 atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg gaaaagaaat
     4021 cgacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca atcggaagcg
     4081 gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca gcgatccttt
     4141 catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat catttaggag
     4201 aaaagcgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca tttggtaaat
     4261 taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga aatgttgctt
     4321 ttgcaacgat gctgatgagc gccatgggag aatttttccc gatattcaaa ggtggacaaa
     4381 atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta ttagtaaaca
     4441 atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa ttagtgccat
     4501 tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc actgctgatt
     4561 tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga acgatctggt
     4621 tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc gaaggcgcaa
     4681 gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg atcattggtc
     4741 tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc gtcttatctc
     4801 aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg
     4861 gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt ggttcttggt
     4921 tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc
     4981 cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta gttgttactg
     5041 caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc tatcaatttg
     5101 cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg atctatcaag
     5161 tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg attgctgtag
     5221 tttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta tgtagtattg
     5281 cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta
     5341 cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt tcaatcgggt
     5401 tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat cgcaaatttc
     5461 ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat tgcacaaagc
     5521 tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag cgtaagtaaa
     5581 tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg atcagatgtt
     5641 ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat cttacaaacg
     5701 aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc aggagaccat
     5761 gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc
     5821 gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt agaacagcta
     5881 gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa caatcctaca
     5941 ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg tacagtagat
     6001 gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga attcttgtcg
     6061 atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac ctactccatt
     6121 ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt ttttcgaaag
     6181 tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca
     6241 ttgaagaata aagaaaaaat attggcgcga aacaaaaaga tcattacaga gaaccttgtg
     6301 attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc caattacgtc
     6361 tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg tatcaattta
     6421 ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc gaagcatgcc
     6481 cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct cttatctgag
     6541 ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa
     6601 gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga agctatggag
     6661 actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt
     6721 acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca
     6781 aatgcactgg aaacactgga gccgatcata ggccctacta cgttaccagt tgcttggaaa
     6841 gattggttat aactgaagat ccgtccagca gcttgatgag gcgaatattt tgtgatcaat
     6901 gcttggacag cagggagcag acaagcatca gagataccaa tcaaaaaacg taaggcagct
     6961 aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc aaaacccaaa
     7021 ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa tcttggagcg
     7081 gctatcaaag tggcaatccc aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc
     7141 ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat ggaattgttg
     7201 gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg atatttcaag
     7261 tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac aaatttttca
     7321 tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt aatgaaaaag
     7381 gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt
     7441 cctgtaacag aaccagttgc taaggttcca agaacttgac cacttttttc ttttggcgtc
     7501 ccagttgcga caagggcagt ggcattactg atataaccag aaaaaatccc ttgaagcaga
     7561 cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat gacgattgcc
     7621 attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtcggc taattttccc
     7681 caccaaggag agataagcgt agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa
     7741 aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga cataaaaggc
     7801 atgactaaac taaatccgat accagccata aatgttccaa accataagac aaacaagttt
     7861 tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc caattagact
     7921 ccttctttct gaaaattttc atctacagct ctttattata aactacaata tgtagtttat
     7981 caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt gaaggattta
     8041 ttttatgaga ggaaacatgg aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga
     8101 ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc agcatttagc
     8161 caaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt
     8221 caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc gacgggctct
     8281 tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc gatcctttgc
     8341 tttttcaaat caagcagtgt atgagttact gatcagtgta ccgcatacca atgaagctgt
     8401 tttattagat ggaatccatg aaacgaatca gatcattttg cagatatttg aggcttttca
     8461 attaacgaaa gaagaaaaag ttcatcgaag cagggaactt cgtagtatga tccacggtta
     8521 tctttcctta cgttttttag gatactttac aaaagaacca acaatcgatc cagaagacag
     8581 ctatttttgg atgatcaata actttatcgc tactttgcct taaacaatca gattcatttg
     8641 taacaatttt aagatgttca ttaaatgacc cttttataag agaaaattct tgcaactttc
     8701 ttgaaaagtg actaagatga gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa
     8761 gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg ggaagaagat
     8821 attgaagaag aagaaacatt cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa
     8881 tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc
     8941 ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt acagcaatac
     9001 aagggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcag aaaggaattt
     9061 tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct
     9121 tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg ttacttgggc
     9181 ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat ggcagagctg
     9241 gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac ttcctaaaac
     9301 aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct cagaagcagt
     9361 cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct tagagtatgt
     9421 gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata aagcaatcaa
     9481 aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg aatctgctct
     9541 tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct taactgtgaa
     9601 gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat tagtgagaga
     9661 ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca cgttgcaagc
     9721 tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga
     9781 agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag aagtccaaga
     9841 aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc aaagagaagc
     9901 caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga
     9961 gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa atgacatgaa
    10021 aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg tcaagcagta
    10081 tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta aagtgaccga
    10141 tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga
    10201 gcgaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc aaccccataa
    10261 agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa
    10321 tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa cagtcaaatg
    10381 ggaaaaagga atgagaatca atcccgattt cgccatcaaa aaagaactat ctgcaccgcc
    10441 taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc
    10501 agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg aacgtacccc
    10561 tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa atccctcttt
    10621 agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg ctgcaggaaa
    10681 gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga
    10741 gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga aatgcccaga
    10801 atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg tctgcaccaa
    10861 ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca
    10921 atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc gttgtaaata
    10981 cgatggaacg acagaaaaat tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca
    11041 tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag aaagcccatt
    11101 agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg ttgtgataga
    11161 agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    11221 aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg aaaaaaggac
    11281 tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact tcatttgacg
    11341 tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct cttatgatat
    11401 aaacaggacg ctgtttcgtt tctaagaaga ttttcccaat atatttcccg atgattccaa
    11461 gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa ggccatcctg
    11521 atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca ataccagaac
    11581 cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc gagaagttca
    11641 cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt tcgccagcca
    11701 cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag ctaaataatc
    11761 cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt tgacgagtca
    11821 tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta ttgatcaact
    11881 ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga gtcgaacgcc
    11941 gggtgccgac acaatcgata tcagaagtag tgatccggtt gatcatctca ggaagcagtt
    12001 cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta gaagcttgca
    12061 atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca aagcggacat
    12121 aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt gatccgtcgt
    12181 caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca tcaaaaaaaa
    12241 gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc atcgtttcta
    12301 tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac cttaaataac
    12361 acctttaaaa tttgatttta cgtaaaagaa caggtattct ttttgtaaac taacgtattt
    12421 agtttaccat gtttcctcgg tttatcaaac ttttaagaac aatttttatt agttggcaag
    12481 tagttttgga tagagaaaaa ggaggaagtt ccagtctatt gactaggagc aaacaaaatg
    12541 aaaatacaca cacctatcgg ctttttatgg ttagatgcag atgaatccgg attgactgct
    12601 gtatcttttc agccaatttc agaaagcgaa tcagcgaatc agcgaatttt aacgcgcaca
    12661 caagaacagt ttgaagaata ttttgcggga aaaagaaaag aatttgatgt tcctttatct
    12721 ataaagagag gtacgccttt tcagcaaaat gtctggaaag ctttgtcgac cattccttat
    12781 ggagaaatcc gaacgtataa agaagtagcc gttgctgttg atagtccgaa agctgtgcga
    12841 gcgattgggc aggcaaatcg aaccaatcca ttgccaatcg ttattccttg tcatcgagtg
    12901 atcgggcaaa atggaaaact gacaggatat atgggaaacg caatagatgg ggttgaaatc
    12961 aaacgccaat tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga
    13021 cattgttcca tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc
    13081 aaataaatag taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga
    13141 agcgtgtacc ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag
    13201 cagtgatctc aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag
    13261 aaaacaagct gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta
    13321 attttcgttt tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca
    13381 tgaatttttc aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata
    13441 gcatggcgaa ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa
    13501 cagattggag tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact
    13561 gtaatccaag aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga
    13621 tgatctgtcc tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag
    13681 aagaaccaca aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca
    13741 gttttttacc aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa
    13801 caattccttt tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa
    13861 aaacagagaa ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct
    13921 gtttaaaaaa agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta
    13981 actgtggcag ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta
    14041 aaccgggtaa gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg
    14101 atttcatttg atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc
    14161 actaaaccag tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct
    14221 tgtttcgaat aaacggtgtt ccagaagtag cttct
//
LOCUS       DACEJZ010000078        14116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEJZ010000078 DACEJZ010000000
VERSION     DACEJZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.081"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..554)
                     /locus_tag="IW656_002163"
     CDS             complement(<1..554)
                     /locus_tag="IW656_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0837858.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(570..1049)
                     /locus_tag="IW656_002164"
     CDS             complement(570..1049)
                     /locus_tag="IW656_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0837859.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1055..2079)
                     /locus_tag="IW656_002165"
                     /pseudo
     CDS             complement(1055..2079)
                     /locus_tag="IW656_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2096..3202)
                     /locus_tag="IW656_002166"
     CDS             complement(2096..3202)
                     /locus_tag="IW656_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0837860.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="IW656_002167"
                     /gene_synonym="adhC"
     CDS             complement(3431..6028)
                     /gene="adhE"
                     /locus_tag="IW656_002167"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0837861.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="IW656_002168"
     CDS             complement(6333..6713)
                     /gene="yajC"
                     /locus_tag="IW656_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0837862.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="IW656_002169"
     CDS             complement(6810..7955)
                     /gene="tgt"
                     /locus_tag="IW656_002169"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0837863.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8173..8871
                     /locus_tag="IW656_002170"
     CDS             8173..8871
                     /locus_tag="IW656_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0837864.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9039..11678)
                     /locus_tag="IW656_002171"
     CDS             complement(9039..11678)
                     /locus_tag="IW656_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0837865.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12297..13328)
                     /gene="queA"
                     /locus_tag="IW656_002172"
     CDS             complement(12297..13328)
                     /gene="queA"
                     /locus_tag="IW656_002172"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0837866.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13521..>14116
                     /locus_tag="IW656_002173"
     CDS             13521..>14116
                     /locus_tag="IW656_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0837867.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3957 a         3096 c         2543 g         4520 t
ORIGIN      
        1 gcgcctacga gataacctcc cataaacaaa gcagagatcg aagcaccacc agcaacaagt
       61 gaatacatga taaaagcagt acttggagga atgaccatac ctgtcggagc tgacgcgata
      121 ttgaccgctg tcgcaaactt ccggtcatat ccttcttcca cttgctgagg gatcaacact
      181 ccaccaatcg cggtagaagc ggcaatggcc gagctagaga tggaaccaaa taaagcattc
      241 cctaacacat tcgtatgagc caaagccccc ggcacgcgcc cgacgaacaa catcgcaaaa
      301 tccactagtt tcttagctat ccctccatta ttcatgataa tgcccgagag aacgaaaaac
      361 ggaatagcga tcagcgaaaa actattgata cttcccacca ttttttgagc tgaactaaaa
      421 gcagaaacat caaacggtat aactaacaat aaagtcatca tcgatgagaa tgcaatactt
      481 atagcaatcg gcatccctaa tacaagcaac acaacaaata caaaaaataa cactaatccg
      541 gcttctattg ccattttatt cctccatttt tatatttttt cttgaattgc tggcttgttg
      601 cttaatgtat cgattagatt gatcaggcta tagataatga tgagtacccc cgatacaggt
      661 actgacagat aaacatatcc cataggaata cttaaagaag gcgagatctg ccccattgtc
      721 aatgtgacag cattccatcc gccatacacc atgatcatcg cagcaaatac cagaaataaa
      781 ctttgaacaa caacttctaa aattttactt tttttgccag ttaatttttc acttaaaacc
      841 gttacggcta aacgtgcttt ttgtcccaca acatacgcgg aagacaacat ggcaaaccag
      901 acaagaccga atcgaacaag ctcttccgtg accgtatttg gatttcctaa aaccgttctg
      961 gaaaatactt gccataaaac aacagcgacc atcccaacaa gcaaaacagc tcccaggatc
     1021 tctaatacct tgtttaaaat cattttcatt acagctacgc tccttgctct tggatctttt
     1081 gataaaggtc tttgtaggta ggatcgtttt tgaatatatc atgaagtggg gcaactgcct
     1141 ctcgaaacgg tgtattatct acatcattga attggactcc atgttcttct tgtgctaaag
     1201 ctttttcttc ttcaattgct tttgcaaaag cttcaatttc aaaaaccgtt gattctttcg
     1261 ctgcttcttc aatggctttt tcctgttcat cagttagctg ttctttgatg gcatcattca
     1321 tgatgatgat atctggcaca cgtgtatgct catcatagga atagtattta gctaccccgc
     1381 catgtccagc tgtatacaag acaaattcat tgttttctga tccgttgatc aaattggatt
     1441 gaagagacgt gtacacttca tcacttccca taggtacggg cgaaccgcct aaaagctcta
     1501 ccattttgat agcggtttcg ctttgcatca cacggatctt ctttcccttc aaatcttccg
     1561 gcgtatggat cggaccgtct accatataaa agctgcgctg accagaatca taataggtca
     1621 atccggtaaa tcctaaattt ttggtcgatt ggtagattgg ctccattatt tcttcattat
     1681 ccatcacctt aaagaaatgt tcttcatcat tgaatagata aggaacggaa aaaatcgaat
     1741 aatctttaga gaaactttcc aacgctgagg cacttacttt tgtgaaatct actgctcctg
     1801 actgtgtcaa ttcgatcagt tcacgttctc cacccagttg tccatctggg aaatactgga
     1861 tctgtacgga accatctgtt ttttctttta ctaaacgccc aaattcattc attgcatcaa
     1921 ttactggctg ttgcttgcat aagcaaagcg caaggtaact atctcattta cgtctgcatc
     1981 tgcgacagtt gccacactag aattatttcc gcagccgctt agacctagca gacctaggct
     2041 aagcgaactt gctgcaaaca gcagtgatgt ttttttcata tgggtttcct ttcttttaac
     2101 aataagagac gagcaattct gtcaaacaaa gcaccgttaa cgactgtcca taaggcattg
     2161 ctgtgattcc gatattccga taaaaatcta aactgtcccc cattcctgtc ccgattgaaa
     2221 cgttttgaac ttctcctttt tcatcgattt gttcaagcaa tccttggata gcacgataag
     2281 cgacttcttt atattcttgt ggtaaataac gtttatggac cgctttcaaa ataccgtaag
     2341 caaatcctgc agttgcagaa gattctacgt aagaagaagg atcatccaac aacgtatgcc
     2401 ataaaccgct ttcagactga taagttttta atgcacgcac ttgtgcttca agtgtttcga
     2461 tcaataattt gcgcaatgca tcttctttcg ataattctaa gatttcaatg atctctggaa
     2521 tagcaatcgt caaccagcag tttccacgag cccaaaatgc ttcggcatag ttatgatttc
     2581 cttcaaacgt ccagccgtga taccacaatc ctgttcgttt atccatcaag tatttgatat
     2641 ggatcataaa ctggtaacga gcttcttcta aatattctgg gcgattcaac agtttcccga
     2701 tttttgccaa aggaagcact gtcatcatca atgtatcgtc ccacaactga ttcttatttt
     2761 ccggtccata tgtggcatgc tgtaacccgt cttcatttgt ccgaggcatg tcattcatga
     2821 cccattctgc ccattgttct aaataaggca aatagcgtga atctttcgtg tcttcataca
     2881 agtaagccat agtcaatagc ggtgccattg tattgacgtt tttgggcgga gcgccttctt
     2941 ccatacgatc ctcaaaccat tcattgacca cttgataagc gcgaggattt tttgtcatct
     3001 ggtaattttt ataaatacca tatagaccga caccttgtgg ccaattccac actgtccagc
     3061 ttttgtcatc tactttcagt ccatcaaagt ccaacaaaaa ttcaccatta tcgtctttga
     3121 tcgttgtcag gttttcaacg agcaggtcaa ttttttcttt cactaattct ttaggtacaa
     3181 aaatctcttc tttaataatc atctttacgc caccttttta aacgctttca ttttatatcc
     3241 ttaatatagc agtaataccg tccactataa atatcgcttt ttgaacaaaa actgtctaaa
     3301 attgacctaa atgttaaata acaaaagtaa ctttcagtta cttggaaatt aaaaaaagaa
     3361 cttggagaaa caacgttttc caagttcttt ttcatctatt ttcttatctt ttttctacgc
     3421 tttcttttgc ttatccgttg taagcatcaa tgatgatctg tttcaattca gagatcaatg
     3481 gttctttcgg attagctgtc gtacattggt cttcgtaagc taattcagcc atacggtcta
     3541 cggttgtatc tagtgtttct tgtgtgactc cttgagcttt tagactcatg ttgatgccga
     3601 cagattttcc taattcataa acagccgttg ctaaagcttc gactaattcc tctgttgttt
     3661 cgcctttcag acctaagaat tttgcgatat ctgcgtaatc tgtatctgca cggaaatagt
     3721 catatttagg gaacatcgca tgtttttgtg gatcttttgc attatagcgg ataatatgcg
     3781 gtaaaagaat cgcatttgtc cgtccatgag ggatcccgta ttccccgccg attttatgtg
     3841 cgactgagtg acaaatacct aggaatgcgt tagcaaacgc cataccagcc attgtagaag
     3901 cgttgtgcat tttttcacgt gattccatat caggtgtttt tactgatttt tctaaatagt
     3961 caaagactaa cttgattgct tgtaggctca atccacgtgt ataatctgaa gccatgactg
     4021 aaacatagga ttcgattgca tgtgttaaca catccatccc ggtatctgct gtgacagatg
     4081 ctggaactga catgacgaat tgaggatcga cgattgcgac atctggcgtc aatgcataat
     4141 ctgctaacgg atatttcaca tgtgtttcac tgtcagtgat gaccgcaaat ggcgttactt
     4201 cagaaccagt ccctgatgtt gtcgggatac atacgaattg tgttttttct ggtttgtcga
     4261 tcttatatgt ccgtttacgg atatccaaga atttttgttt tgcaccaaag aaagatgtgt
     4321 ctgggtgttc atagaacatc cacattccct tagctgcgtc cattgcagaa ccgcctccaa
     4381 gagcgattac tgtatctggc ttgaagtctg ccatcagttt cgttcctgcg tatacggtat
     4441 ttgtcgatgg gttcggttca acatctgaga agacttcgat cttcacatca tttttacgtc
     4501 gtgccaatac ttcacgaact gtatcgcagt aaccgaattg gaccatacca gggtcacaaa
     4561 cgatcatgac acgttcaacg ttttccattt tctctaagta caatagagag ttcttttcaa
     4621 agaagatttt tggcggtaat ttgaaccatt gcatgttatt tctccgtttc gctacagttt
     4681 tgacattgat taagtttaca gcagagacgt tacgagaaac agagttctta ccataagaac
     4741 cgcatcccaa agtcaatgac ggaatcattt cgttatagat atcaccgatg ccgccttctg
     4801 cagatggtgt attcactaaa atacgacaag ctttcattcg aagaccgaat ttgacatgca
     4861 actcttcgtt ttctgaatgg ataactgctg tatgtcctaa tcctccaaga ttcaacatac
     4921 cttcacacaa atcaaacgca tggtccgtgt tgttagcttt gatcatggca agaactggag
     4981 aaagtttttc tcttgatagc ggataatctg ggcctacgcc ttccaattca gcaatcagca
     5041 ttttcgttcc ttttggtaca ctgattcctg ccaattttgc gatttccatc gcagaatgtc
     5101 caacgattga tggattgacc gcatattttc cttcgttcat cactgcatct tctaattttt
     5161 gcaattcatc tggttttacg atataaactt ggtgtgcttg gaattctgct tttactgctg
     5221 catagatttc tttatccacg atcacagctt gttcagaagc acaaatcatt ccattatcaa
     5281 atgttttcga aacgatcaaa tcgttcactg cacgtttaat ttttgctgtt ttttcaatat
     5341 aagctggtac gttacctgga cctacaccta aagctggttt tcctgtagag taagcagact
     5401 tgaccattcc agctccacct gtcgctaaga cgatcgcgat acctggatgg ttcattagca
     5461 tagaagtcgc ttcgatcgaa ggatgttcga tccattggat acaattttca ggagctccgg
     5521 cagcgatagc agcatcacgt accactcgtg ctgcttctgc agaagatttt tgggcacttg
     5581 gatggaaagc aaaaacgatt ggatttcttg ttttcaacgc gatcattgat ttgaaaatcg
     5641 ttgtggaagt cggattcgtt gttggagtta ctccgcaaac cacaccgact ggttcagcaa
     5701 tttcaatcaa ccctttttgg acatcttcat tgatcacacc aactgtttta tcatgtttga
     5761 tattatgcca aatatattcg gacgcataca tatttttgat tgctttatct tcgtaaacac
     5821 cgcggcctgt ttcctctaca gccatcttcg ctaatggcat atgttgatcc aaagctgcca
     5881 ttgccatttc atgcacgata tggtctactt tttcttgatc aaagctttcc atttctttca
     5941 atgctgcatt tgctttttct gccaaatcat caatcattgc ttgtacgtca attgtcttcg
     6001 ttttttcttt ttctattact ttagccatca ttatcctcct aatgggtcat ggttatttgt
     6061 taaataattc acatgtatat catacaatat ttatttttct atgtaaacac ttttttgtga
     6121 tttttttcac agttattttt aaatataaaa ttgctttatt tacaggtgtt tgtatagaaa
     6181 aatatataaa gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa
     6241 tatatcccat gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct
     6301 gccctacctc gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct
     6361 tccagcgttt ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt
     6421 tcaacagtag tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct
     6481 gcacgatcgt actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg
     6541 tcgatttctg aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc
     6601 atgctatcta acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa
     6661 aaccacattg ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc
     6721 acctcactaa ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt
     6781 tttttactta atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac
     6841 ccgtattctt caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga
     6901 cgtacttgtt tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc
     6961 ccaaatgttt catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat
     7021 gtataacaat cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc
     7081 actactagac ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag
     7141 acacagtcaa acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg
     7201 acacccatca agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca
     7261 cgattcattt ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa
     7321 tccatcccga ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg
     7381 atgattccaa ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc
     7441 catcgagacg tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga
     7501 cattcgtcaa aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt
     7561 tctggtgata aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct
     7621 tcgatattcc gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc
     7681 ggttgatccc agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca
     7741 gggcgtagcc ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac
     7801 tcttctgggg acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg
     7861 gggaaagttc cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc
     7921 ttgattaaac gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa
     7981 tatcttgaca actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct
     8041 tccttctact ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag
     8101 aaaaacatta tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag
     8161 gagggaaaaa tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa
     8221 ggacgttggg gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact
     8281 attcttctta ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg
     8341 agcgtctcag aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt
     8401 tccagcatca ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta
     8461 cgtggacaaa aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg
     8521 tttatcggcg gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt
     8581 ttgctattag ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt
     8641 tactacgata tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca
     8701 agtcgaaaat tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc
     8761 ggctggcata tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac
     8821 atttctgcaa caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg
     8881 tgacttgtga ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac
     8941 attcagccca ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat
     9001 agttccagta aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc
     9061 ggtaatcgat ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct
     9121 accaagaacc agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc
     9181 agcaaagcaa cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta
     9241 ttggtcgcta aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga
     9301 atcgatgaat aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct
     9361 acccatgggc tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg
     9421 gtgaagaccc ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca
     9481 tttttcggaa tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca
     9541 aatcctggaa gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt
     9601 gctccccatc ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt
     9661 agcaaaaagt tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg
     9721 tctacgatcg tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca
     9781 tctgttcctg tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca
     9841 ttcacaccat ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc
     9901 cggatcttgt cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct
     9961 aattcttcgt ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc
    10021 aagataccta attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg
    10081 actgttttga tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt
    10141 ggatcgatca ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa
    10201 atatcttgcg gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt
    10261 ttaccaaagc gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga
    10321 gcaagaacat cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact
    10381 gttgtcatca tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct
    10441 tctaattctg cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt
    10501 ggattacctt tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt
    10561 gcagccattt tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact
    10621 tcatcttctt tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt
    10681 ttgtctgagc agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg
    10741 atcgcatgtt tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca
    10801 gtcaaggttt ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg
    10861 agtggttctc cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc
    10921 atactgattc gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta
    10981 ttttcgttca aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg
    11041 atcgctttcc cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca
    11101 cctaatgact catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct
    11161 ccagtcaacg ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca
    11221 gaaatagcat caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca
    11281 acagtggtca ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag
    11341 gattccagtg ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca
    11401 acgatagcga tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta
    11461 taaaaggaaa tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga
    11521 aagaattttt tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt
    11581 tttcgccgtt catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc
    11641 ttctttactt cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt
    11701 cgtacaaaaa aaggtactta ttttactata acatcctttt tttatttact tcaaataaag
    11761 ggcttatcga ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct
    11821 accaaaggat gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa
    11881 tttctcgata gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca
    11941 tgcgtgtaga gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta
    12001 tccgaaacaa gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga
    12061 ataggaacaa gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac
    12121 atgaagaaat tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt
    12181 atttgaatcc ttccgaggta caagaaagcc gagcttatcg agagctcggc tttttctttg
    12241 tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc ataaccttat
    12301 ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat cgcatgctgg
    12361 taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac taaagtcgat
    12421 tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata gccaggcgta
    12481 atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt cgttccaatc
    12541 gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc atttttacgt
    12601 acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact atgcatatgg
    12661 tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac atgtagtgtc
    12721 agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc
    12781 aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt ttgatagcga
    12841 tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc caatgattca
    12901 agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc gtgttccaat
    12961 tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt cccaactttg
    13021 gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt cgttagaaga
    13081 aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc aggcagaact
    13141 cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat ttcatggaaa
    13201 tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga atgatcgcgg
    13261 tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa atcttctgtt
    13321 gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat gtttttcgtt
    13381 ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat aaaaaaacta
    13441 ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc ttcattgaaa
    13501 atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg
    13561 gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc atttgtttta
    13621 tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga atgactgatc
    13681 caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat ccagtggaat
    13741 tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac atgacgatcc
    13801 gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa
    13861 cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat cgctacccac
    13921 atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc
    13981 aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact cgtgattcct
    14041 tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc
    14101 atgacatgga tgaagc
//
LOCUS       DACEJZ010000079        13990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEJZ010000079 DACEJZ010000000
VERSION     DACEJZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.082"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..777)
                     /locus_tag="IW656_002174"
     CDS             complement(121..777)
                     /locus_tag="IW656_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0837868.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            970..1248
                     /locus_tag="IW656_002175"
     CDS             970..1248
                     /locus_tag="IW656_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837869.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1267..2475
                     /locus_tag="IW656_002176"
     CDS             1267..2475
                     /locus_tag="IW656_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0837870.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2623..2988)
                     /locus_tag="IW656_002177"
     CDS             complement(2623..2988)
                     /locus_tag="IW656_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0837871.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3135..3909)
                     /locus_tag="IW656_002178"
                     /pseudo
     CDS             complement(3135..3909)
                     /locus_tag="IW656_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(4083..4943)
                     /locus_tag="IW656_002179"
     CDS             complement(4083..4943)
                     /locus_tag="IW656_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0837872.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4943..6361)
                     /locus_tag="IW656_002180"
     CDS             complement(4943..6361)
                     /locus_tag="IW656_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837873.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6418..6867)
                     /locus_tag="IW656_002181"
     CDS             complement(6418..6867)
                     /locus_tag="IW656_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0837874.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7312..7575)
                     /locus_tag="IW656_002182"
     CDS             complement(7312..7575)
                     /locus_tag="IW656_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0837875.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7674..8969)
                     /locus_tag="IW656_002183"
     CDS             complement(7674..8969)
                     /locus_tag="IW656_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0837876.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8966..10237)
                     /locus_tag="IW656_002184"
     CDS             complement(8966..10237)
                     /locus_tag="IW656_002184"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0837877.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10402..11271)
                     /locus_tag="IW656_002185"
     CDS             complement(10402..11271)
                     /locus_tag="IW656_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0837878.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11563..13173)
                     /locus_tag="IW656_002186"
     CDS             complement(11563..13173)
                     /locus_tag="IW656_002186"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0837879.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3982 a         2947 c         2387 g         4674 t
ORIGIN      
        1 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
       61 ttttccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttgttca
      121 tcaaaagata atattgtctt ctaacaagtc catattcaaa ataacgatat gatggtcttt
      181 aacggtgata ataccatctt ttttcagttg ttttaaaatt ctgtttacac tgctagcagt
      241 tgagatgcca caaaatttgg cgatttcttc attcgtgatc gaaaaatcga tcagcatccc
      301 ttcttctgtc cgcacaccga ataaatcata taattcgtaa agttgcgtac aaacagctcc
      361 cagctttccg tttaatagca tttgctgcat ttttttcatc gaataaagca gacgatagcg
      421 gtaatactct tttacatagt gttgtagctt tgggtcttgg ttgatatttt gccaaaagcg
      481 cacacgattg attcgataaa actctgcata ttctgattca actcgaatat tatacggcga
      541 atcgatgaat tgtgaatatt cgtcttttaa aagagagacg atttcaagat ctttgatata
      601 gcgaaggtta aattctctcc catcatttga aataacactt gcttttaccg ttccctcttt
      661 caagatatac gtatgggaat cttccatccc ttcgaatgtc aaatatttat gatatccttt
      721 gcgtataatc ggaaaatcat ttttatccaa atattccttt aagactctac tatccaatga
      781 tgctccccca tccaaacttt catctaaaaa gatcatacag tactttaaaa ctttttaaag
      841 tccaaaggta ttttactaat gatattaaca tttgttaagt acctttttcc atcacttatg
      901 cgattttttt cctttttttt attcgttata ctgcagaagt aaaatttttt aagaatagag
      961 ggaaaaaaca tgaattactg gatgaacaca atcattaatc gattagaaac agcatatcaa
     1021 acaagatttg atatgaaagc ttctttagtc tttttaaatg atgcgtatca aaactcgatc
     1081 gaattgatca aagcagtgga tgagaatcca acaaacgaat gcgaagaatt tttaaatctg
     1141 tttatgtcta caagagattt atttattcgc caacttgttg atcgctatcc atccaattat
     1201 cacgatgtag aagttcaaat acaaaaatta aaagcatatt cagcttaagg ggaatgtata
     1261 acatacatgg aaaagaaatt atttaataaa tattttattg gaatcacatt gatcaatttt
     1321 attgtctacc tgatttatta tctgttgatg gtcatcatgg cagtcattgc gcaagacacc
     1381 ttacacgcca gcttgagcca agcaggtttt gcttctggga tctatatcat cggaacattg
     1441 ctggcacgct tattcatggg gaaacagtta gaactatttg gtcggaagtt taccttaaga
     1501 ggcggcgcga tcttctatct cctttcaaca ttagcatatc tttatacgcc aactatcggg
     1561 gcattgtatt tagttcgatt cttgaatgga ttcggctacg gtacagtttc tacagctaca
     1621 aatgctatcg ttactgcata tatccctgca tctaaaaaag gggaaggcat caattattat
     1681 ggattaagta ctagtttagc tgccgctgtt gggccatttt taggaatgat tttgctgaat
     1741 atgactgatt ttcgtttcat tatctggttt tcaattgtac tggtcttctt tgttacgatt
     1801 gcctgccttg tctttcctgt aaaaaatatt ccattatctg aagaacaaaa aatatctctt
     1861 cgctcatgga atatcaatag ttttattgaa aagaaagcac ttttcattac aggaattgcc
     1921 tttttgatgg gtctttctta ttcaagtgtt ttatcatttt tatcttctta tacaaaagta
     1981 atccacttag ttgctgctgg gtcatttttc ttcgtcgtat atgctttggt catcacattt
     2041 acacgtccat tgactggaag gattttcgat gtcaaaggag aacaatacgt gatgtatcct
     2101 agctatttat tcttaacagc aggattattt ttactgagtg taacgacgaa tagtgtgaca
     2161 ttgcttatct ctggagcatt agtcggccta ggttatggta cgtttatgtc aaatggacag
     2221 gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta ttgctttatc tacctacttt
     2281 attggtttag acctaggctt aggcgttggt ccttatttga tggggatgct aaaatctctt
     2341 acttcattta gaggattgta tgtgattgcc ggagtcattc cattgatatg tacagcactc
     2401 tatcttgtta atggccgaaa acatagtcat gaagcagagg gcgaattgat tgaatcattg
     2461 aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc gttcagatct gaaaataatc
     2521 tgagcggttt tttgagtaaa aatagtttat gattattctt aattaaacat ctatttattt
     2581 ttaaagcttt catttatcga taagaatagt ttaactgttg atttattcca ttttccgttc
     2641 atctggtacg atgccttgac ttagcaacca cttgtggaag aaccattctg ccacaccgat
     2701 caatagagca ggaatcaata ctttcatcca aatatcgact gtatcattca taaaatatag
     2761 atagagccac aagataactg cagctgaacc agcatctgcg atcgtcgctg cgatgttccc
     2821 tactttacgt aagatcaaca gatcccctag cacatagatc atgactacaa taattacccc
     2881 aacaatcgtt gaatccataa agctaacacc gtataaacct gtcagcacga cccacagaat
     2941 cgcccagatc atgattgcct tcactaccaa cgccttcaca tgtttcattt tctgcactcc
     3001 tttctcctgg ataaaaaagt aagattatct aacaagataa ccttttcatt attataccat
     3061 cggctgagaa aagcgagcaa agataaggtc ctatctaaaa atgaggaagc cacttagaac
     3121 attcacgtaa taatttatcc gtacagacat tcgatatggt gttcctttac tttcgcatac
     3181 tgttcttttg tcagttcttt acttgtcacc agataatcga tttcatgaaa agcaatggct
     3241 tgatgcatga atttatgtgc aattttttgc tgtgtaacta aaacggcaag ccggtctgat
     3301 tgttttctaa aagctttttt tgaccattgc ctctccttca tgagtttctg ttactccttg
     3361 gctgaaatct agcccgcgac aactcattat cgctaaatcc gcataatatg acaatgcatc
     3421 gttataggct tttgaaccgt tgattgttgc tcgtttcgga acaattgccc ctcccaatac
     3481 agatactttt gccgttgtat attcactcaa catagcagct gtgacgatgc cattcgtaat
     3541 gatctgcaaa ccatcaaatc ttgctaggtt tctgactaag tgtaaagagg ttgtactgga
     3601 atccaagaat aagaccatat catctcgaat caaagaagaa gctgtatctg caatcttacg
     3661 tttttctgtt tcttcttcta atttggtgat cttcatcagg taaggcacgt caatttttcg
     3721 attgtcaata agcatggcgc caccgcgaac tctacggatc aatccacttt tttccagaaa
     3781 aacaaggtca cgtttaatcg tggcttcgct ataatttact aactcgatca aatcggtgac
     3841 tcttgcaaat tggttgtttt ttagataatt cacaatctcc tgctgtctat tctccacttc
     3901 attcaacata aaatcacccc atttacttta tatagtaaca ggaaaaaaga ggttgtgaca
     3961 gaagtgattg gcttcgagaa ataagaagaa atttttcgaa aaaactactt ctgaacatcg
     4021 tgtgtggtta agagggtatg acaagactat tgtcataccc tcttgaccac acatttttat
     4081 ttttagtata aactagcctt gcctactgtg ttcaaaattt ccattttatg tttgaccact
     4141 tcttgtacct ttttattagc tgctggataa attgcattcg gctcgtacat cgatggattt
     4201 tcactcaata tttctctcaa agcattgaaa aagacgcttt taagatctga gctcaaattc
     4261 actttggcaa ctccgtattt tactgcttcg ctgacttctg cgtccggatt acctgaaccg
     4321 ccatgtaaaa caaatggaag atcgatttgt tggttcaatt ctttcagtaa gtcgatattt
     4381 aattttggag ttctgttttt cggatacaag ccatgcgctg ttccaattgc tacagcaagt
     4441 gtatcgattc ctgtttcttt cacaaaaatc ttcgcttgat caggatctgt ataaatgatt
     4501 gtttcagcac ctccttcagc agaaccatta ttcccaatcg ttccaagctc agcctccact
     4561 gagacattta ccgcatgagc taacgcaacc acttttttcg taatctgtac attgtcgtcg
     4621 aatgatttca aagacgcatc gatcatcacg gaagaatagc cgtttcgaat cgcccgtaag
     4681 acatcttcta tcgtaccgcc atgatccaag tggataacga tcggcagttt acttgtataa
     4741 gcataggctc ttactgccgc aacaaactca tcacttaaat aggcgatttc atcaggatga
     4801 atttctaaga ttactggtgc ttctaatctc tctgcttctt cgatgattgc tttcatcata
     4861 tcatagctgc aaatattgaa ggctggtact gcaaattgat tctctttagc cactttcaat
     4921 aactctttca ttgtatataa cattacgctt ccacctcatt tgttagattt tcatttttcg
     4981 tccacttgtc ttgttttttc tctttcactg tttttttcaa tagtccaagc ataaaggctg
     5041 tagtgactgt acctgatgca attgccaaga catacatagg taaattgttc ataacatttg
     5101 gaattagcat cacccaaatc cctccatgag gtgccattga agtaactcct actgccatgg
     5161 aaatccctgc agcgactgct gaacctgtca tcaaactagg tatcacgcgg atcgggtcag
     5221 ataccgcaaa cggtatagca ccttctgtaa tatacgaagc gcctaaaatc cagcatgatt
     5281 gacctgaagt ccgttcttct ttggtaaatt tatgtttgaa taataaagtg gctaaagcaa
     5341 tacctagggg aggtgtcatt cctgcagcca tacaagcagc aatcggtgca tttactccag
     5401 ctgacatcaa accgatcgag aaagtcgaag tcgatttatt gatcggccca cccatatcag
     5461 ctgccatcat gatcccgata agtaatccga acaatagccc ggatgtattt cccaagctat
     5521 tcagccaatt tgtcaaagca tccaacaccc atgtgatcgg ttgttcaatc acaaatacca
     5581 tcaaaagccc aataattaag atcgataaaa aggggacgac aatcagctga taaatagcag
     5641 aaaattcttt ttttacgcta attttatttg ccagaaaatc acagatatac cctgctagca
     5701 cgccaccaat cattccgcca agaaaacctg aaccacctgt agaggccagt aatccggcaa
     5761 caattcctgg ggcaaaacct gcttttccag caatcgatgt agcaatcccc gcgcctaacg
     5821 caggaaccat catggcaaac gctgtatctc ctccgatcct tgaaaatgca gctgctaaca
     5881 cattatagtc tgctgagttg ggatcagccg ctgtaatccc aaaggcaaaa ctcagtgcga
     5941 tgatgattcc gcctgcgatc acaaatggaa gcatgtaatt cacaccattc atgaaatgat
     6001 tatagattgt cggctttttc tcatttgcag ctggttcttt ttcattagac acaacgaata
     6061 cgccaatatc tctcaatgct tcgtctatta ctacttgcgg ttctttgatt gctcggcttg
     6121 tagagacacg ctttactttt ttcccggcaa atcggtccaa ttcgatttct ttatctactg
     6181 caagaatgat tgcatctgcc tctttgattt cttgttcaga taactgattt tctgttttta
     6241 caccttgtgt ttcgaatttt gcttgatggt ttagatttct tgctgttact tctaattttt
     6301 ctgccgacat ataagtatga gcaattccag tcgggcaagc agttacacca acgattttca
     6361 tgattatttt cctccttttt cttcactatc gatactcgga caaagactct actattttca
     6421 ttcttgacta atctgtctga tgatatctgc tggcgtagtg gcttctttta ttccctttct
     6481 aaaatctgca ttcatcaatc gtctggagag tgtgctgaga atctctagat gcatatctgc
     6541 tgatttttca ggaactacga tggcaaaaat atattcaatt ggcatttcat ctatcccgct
     6601 atagtccgtt aatttggctg tatgaaaaaa acacagtgct ggttttgtca catatttgct
     6661 ttttgcatgg ggaatcccaa atccatcttc tattcctgtg gagatctcct cctctcgttt
     6721 gaatagtgcc ttaacgactt tttgaacatt tcttacctta tttgtttttt tcaacacatc
     6781 ccctattcgc tcaaataatg atccttttga atctatcgat ggatcgaaaa caatcagttc
     6841 ttctgcgatc aattgatcaa acctcataaa aaacctctcc cccacaaaaa atgttaacgt
     6901 tttcttggaa ctgaggtcag tatagcatcg ctagctgtac cgttcaatat tatgaatgaa
     6961 cagaggaaaa agacccatca tccgcgtgta taagtcatta aaatgaacga tctagctcac
     7021 aaacaatcgc aagtctataa caaaacaaaa agccttcctt gaaaggaaag cttagcttct
     7081 aattttaggt aaatggtttt ttacttcgaa ataacccctc ttcatttcgt taatataagg
     7141 tgtttttatc aagtggataa ttaaaactat atactagttt ttcattgttt tgtgcattat
     7201 gatcctacga ctttttcagc atcaaacgaa gttttaaatt ggcaaaaaac ataaaaaaac
     7261 agactatccc tatcagatag tctgctaaac gttgagttat cagcgttttt attattccgc
     7321 agcgtttgcg tctttgagac cgtatttttt gttgaaacgg tccacacgtc cgtctgcttg
     7381 tgtgaatttt tgacgtccag tgtagaatgg atgtgagtca gaagttactt cgacacggat
     7441 gactgggtat gtattaccgt cttcccattc aactgtttct tgtgaagttt ttgttgaacc
     7501 tgacaagaat ttgaaaccag ttgttgaatc catgaatact actgggtggt aatctggatg
     7561 gatgttttct ttcatgattt tttcgctcct ttgccctgct ccatttgctg aaccagagtt
     7621 gatttgtcga tttacaacat taacatctta acatgtttca gtcaaggttg caattatctt
     7681 ttcagatttc tggtcggatt ttttttacca aaggagacat cttgaaattc tttgaaaaat
     7741 tgctgattgt tttttgtttt acgcaaaaag cggatcagtt gttccgtata ttctaatgaa
     7801 tctccagtca tattttttcg aagtttccaa atttcttcta attcttcact tggcatcaat
     7861 aattcttctt ttcgggtacc tgattttttg atatcgatgg ctgggaagat ccgacgttca
     7921 gataattcac gtgaaagttg taattctaga tttccagttc ctttgaattc ttcatagatc
     7981 acgtcatcca tacggcttcc tgtatcgact aaagcggtcg ctaaaatcgt caaactgcca
     8041 ccttcttcga tgtttcttgc tgccccgaaa aatttcttag gtttatacaa tgcggctggg
     8101 tcaatcccgc cactcaatgt acgtccgctt ggtggaacga ctaagttata ggcccgagca
     8161 agtctagtaa tactatccat caagataacg acatctcgtt tatcttcgac taaacgcatt
     8221 gcgcgttcca atactaattc agaaactcga gtatggtttt gcggctgcag atcaaatgta
     8281 gaagatacca cgtcgccttt cacgcttcgt tctagatccg ttacttcttc cggtcgttca
     8341 tcaatcagta gaacgattaa ttctacttcc ggatagtttt cggtgatacc atttgcgatt
     8401 tcttttaata cagaagtttt ccctgctttt ggcggtgcga cgatcaatcc acgctgacca
     8461 aaaccgattg gagaaaagat atcgatcatt cgggtagata gtctttgcgg tgttgtttcc
     8521 agttgaagct gtctttgcgg ataaagaggt gttaaggctg gaaaatgcgg acgttctttt
     8581 gcttcttccg gatcttttcc attaacgctt tccacgtgca tcaaaccata ataacgttcc
     8641 gattctttag gtggacgggc ttttcctgcc actttgtccc catttctcaa actgaatcgt
     8701 ctgatttgtg aggcagaaat atagatatct tctgcacttg gtccataatt gattggccgt
     8761 aaaaaaccgt aaccatcttg accaacgata tccaatattc cttccatata ataaaatcct
     8821 tgtttctctg cttgtgcacg aatcacagct agagataatt cctttttatt catcttactg
     8881 taataaggaa ttttaaattc tttggcataa ctataaactt cttttaacgt tttgttctct
     8941 agttctgcca ttgtcaaata atcgctcatt cagctcccac ttcctctatc atttcaattg
     9001 atgcccctaa ggctgttagt ttctcgataa tatgatcata accgcgtaag atataatcta
     9061 cattgtaaat cgtcgttgtc ccttctgcca atagtccagc aatcactaaa caagcacctg
     9121 cccgaagatc agaagccacg acttctgtgc cgtgaagttt atttggacca ttcacgatga
     9181 tcatatttcc ttccacggta atatcagcgc ccattcgtgc tagttccggc acatggttca
     9241 cacgtttcgc atagatcgta tcgatgattt ctgaagtgcc tgtagtcatc aatagtaacg
     9301 cagtaagtgg ctgctgcaga tctgtggcaa atcctggata aggataggtc atgatgtttg
     9361 cgggttttaa ttctttcgag gggtaaactt cgatttcatc ttcgctgatt ttcatatgga
     9421 caccaatttc ctgcaatttt gctataaagc tttccaaatg ttcaaagatc acatttttca
     9481 ctttgatacc atttcctact gcagctgcta atgctagata ggttcctgct tcgatgcggt
     9541 caggaatcat gaaatgccga cagccatgta atgtttcgac tccttcgata cggatcacat
     9601 ctgtacccgc tcctcgaact tttgccccca tattattcag taaagttgcc acatctatga
     9661 tttccggttc tcgtgcagcg ttttcaatta ctgtctgtcc ttttgctttt acagcagcta
     9721 acatcacatt gatcgttgct ccgactgaaa ccacgtccat gaaaatccga ttgccacgca
     9781 atcctttatt ttctgtccgt aaatacatgg caccatgttc atttgtcact tcagcaccta
     9841 gagcttcaaa tcctttgacg tgtaggtcaa tcggtctagg gcctaaataa cagccacctg
     9901 gcaggcctac taccgcttcg ccaaatttac caagcagtgt tcccataaaa taataagaag
     9961 cacgcaaact attgattttt ccgtgcggca tcggaatcga tacgacttct cgaggatcga
    10021 tttctagtat gttgttggaa aaatggacag ttgcgcccat gatctctaaa atttcaatta
    10081 atgaatgaac atcttgtatg tccggcacac cttccaagat cactggggaa tcagctaaga
    10141 ttgcagctgg aatcaatgcg actgcactat tttttgcacc gctgatcgtt acttcaccag
    10201 ctaaaggtcg gtttcctttg attacgattt ttttcatctg tttccacctt gtttattaat
    10261 cttttttcat ggatatgctt gtattttatc atagataaaa aaaagaaacg agcatggtct
    10321 gccgttttaa gtaaaaaaga atggagagcg acttcaaaag tcgttctcca ttcctatata
    10381 tcgtatctgt tatgtggatg cttacgcttt gttagctgaa ccgaaccagt cgatatgaga
    10441 tttagcatct tcaacgattg ctgctttacc tggagctaat aatttacgag ggtcgaatcc
    10501 tttaccttct ttatctttac cagcttcgat atatttacga gtagctgcag caaatgataa
    10561 ttggaattca gtgttaacat ttacttttga gatacccatt gagatagctt tttcgatttg
    10621 atcttgagga atacctgatc cgccatgtaa tactaaaggc atgtctggac ctacagcatc
    10681 agcaattgct tgtaagtggt caaaagctaa tcctttccag ttttctggat attgaccatg
    10741 gatgttaccg ataccgcaag ctaagtagtc gatacctgta gcaaccattt gtttgcattc
    10801 ttcaatatca gctaattcgc cgttaccgat gattccgtct tcttctccgc cgattgaacc
    10861 aacttcacat tcaactgaga taccttttgc atgtgctttt tcaacaacgt ctttagctaa
    10921 tttcaagttt tcttcaaatg gtagatgtga accatcgaac attactgaag tatagcctaa
    10981 ttcgatacat tccaaagcag cttcgtaatc accatggtct aagtggatag caactggaac
    11041 agtgatgttc attgcttcaa ctaaatcagt gattaaatct ttacagattt tgtaaccacc
    11101 catgtattta gctgcaccca ttgatgtttg gataagtact ggtgcttgtt ttgcttcagc
    11161 cgcttcaagg attgcttgag tccattccaa gttgtttgtg ttgaatccgc cgacagcgta
    11221 accgccttta cgagcagctt ttaaaaattc agctcctgat actactggca taataaaatt
    11281 cctcctaagt tttgtaaaac taaatttgtt aagacaattc ttaaccaacg accctatttt
    11341 accaaacatt tcggtagttt tctactaaaa tcaatgaaaa tgaaaatttt caagaagttt
    11401 tttgcaaaaa ataaaatatc caatagcgct ttaatcgaaa aaagtctttt gctttctatc
    11461 ttttttccta caaataataa caaatcagaa aaagagaaaa gtgacaagaa aattgcttat
    11521 ccccttctct tgctactttt ctctttctat caagttcaaa aattattttg tttcgtgatc
    11581 tgctaatgat gctccgacaa atgctttgat taatttttgc gggcgatttg gacgagaaat
    11641 caattctggg tggaattggc atcccacaaa gaattttttc tctggcagct ctacgatttc
    11701 tactaaacgg ttatctggtg agacacctga aaagactagc ccttgttctt caaacaattg
    11761 acgatatttg ttattgaact catagcggtg acggtgacgt tcttgcacaa catcggctcc
    11821 atcatatgct tcagccgtct tcgttccttt tttgatttta catgggtata atccaaggcg
    11881 taaagttcca cccaaattct cgatgttttc ctgatcagcc attaaatcaa taatattgtt
    11941 tgctgtatct ggattcgttt cagctgatga cgcatcttct aatccaacga cgttacgtgc
    12001 aaattcgaca caagccatct gcataccaag acaaatacct aaaaacggaa cgtcattttc
    12061 gcgagcataa cgaatcgctt cgattttccc ttctactccg cgatcaccaa atccaccagg
    12121 aaccaagatt ccatctgcgt ctttcaaacg ttcttctaca ttttctctcg ttaattcttg
    12181 tgctttgatc caatcgatct cgatgtctga attataggcg aaaccagaat gtttcaatgc
    12241 ttcaactact gagatatatg catctggtaa ctcgacatat ttaccgacaa gcgcaatccg
    12301 tacttttttc ttcaagttca agacacgttc ttctaattct ttccattctg tcatgtctgc
    12361 ttctggtgta tctagtttta aatggtcgca aacaatttga tccattcctt gtgcttgtaa
    12421 aactaatggg atggagtaaa gtgtatctac atctggcgac tcgataactg cttctggttc
    12481 gacatcacaa aattgtgcca atttgttttt cacgctttga gaaacaggtt gctctgtacg
    12541 taccaccaaa atattcggtt ggatacctaa accacgtagc tcttttacac tgtgctgcgt
    12601 tggtttagtt ttcatttctc cagcagcttt caagtaagga atcaatgttg tatggatata
    12661 cataacatta tctgtaccga catctgcttt catttgacga agtgcttcaa ggaatggcaa
    12721 agattctata tcccctactg ttccccctac ttctgtaatg atcacatctg aatcagtcat
    12781 tttagctgct cgcatgattt tttctttgat ctcatttgta atatgaggaa taacttgaac
    12841 agttgctccc aagtattcgc ccttacgttc tttacgcaaa acttcagaat agatttttcc
    12901 tgtcgtcacg ttggagtatt tgttcaaatt gatgtcaata aaacgttcgt agtggcccag
    12961 atccaaatct gtttcagcac catcatctgt tacgaatact tctccgtgct gatatgggct
    13021 catcgtcccc ggatccacgt tgatgtatgg atcaaatttt tggatagtta cttttaagcc
    13081 gcggtttttc aataagcgtc ctaaagaagc tgcaacaatc ccttttccga ttgatgaaac
    13141 gacaccgccg gtcacaaaaa tatattttgt cataagttca aaaactcctt tttaatttat
    13201 gtgcaggaaa agtctcagac aataaacaaa gctccctatt cttcaataag aatagggagc
    13261 cgaaatttct gtaaacttct gaccctctaa aagggtgccc aaggtgtata atacaagctg
    13321 actaagggaa agtcaagtac tcaaatgaat actatccgct tacaattttt tctttttcga
    13381 tatcctttga gaaattattt cttaagtcct ttatgagtgc aaaaaaaatt taacccacat
    13441 aataagcttg atataaacat agctaacgtt aaacctaagc aaatcagaaa atcgttactg
    13501 aaaaatttta agtgaaataa tagataaaaa aataaaacaa acattatgat agataatatc
    13561 ccacctaaca aagaactact ttttcctact tgcagcaaca gtttatatag cttatcgtag
    13621 aaacaatctt tttctttcac ccttatttac ctcctttacg taaaaaacgt agtccacagt
    13681 tctcggattt ttggcatatc ttttctatta aataatatca aaaacaaaac aattccacta
    13741 attttttcaa agatgacctt tttttcaaca aaagtgcctt gtagggattc attgaaggta
    13801 acgaatcaca taagtaaaaa ataaggagat gcgacaaaag tcgtgcctcc ttatttctaa
    13861 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat caaacatgag
    13921 aagctgtttt tgaaattatt tcttgtgatt cggagttaaa tggtaggctc acaacctctg
    13981 gataaacggt
//
LOCUS       DACEJZ010000080        13694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEJZ010000080 DACEJZ010000000
VERSION     DACEJZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.083"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW656_002187"
     CDS             complement(221..1177)
                     /locus_tag="IW656_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0837880.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW656_002188"
     CDS             1281..1883
                     /locus_tag="IW656_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0837881.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW656_002189"
     CDS             complement(1971..3470)
                     /locus_tag="IW656_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0837882.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW656_002190"
     CDS             complement(3486..4010)
                     /locus_tag="IW656_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837883.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW656_002191"
     CDS             4308..5057
                     /locus_tag="IW656_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0837884.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW656_002192"
     CDS             5048..5833
                     /locus_tag="IW656_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0837885.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW656_002193"
     CDS             5802..6518
                     /locus_tag="IW656_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0837886.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW656_002194"
     CDS             complement(6620..7639)
                     /locus_tag="IW656_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0837887.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW656_002195"
     CDS             complement(7658..8716)
                     /locus_tag="IW656_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0837888.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMIGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8938..10086)
                     /gene="nagA"
                     /locus_tag="IW656_002196"
     CDS             complement(8938..10086)
                     /gene="nagA"
                     /locus_tag="IW656_002196"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0837889.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10162..10998)
                     /locus_tag="IW656_002197"
     CDS             complement(10162..10998)
                     /locus_tag="IW656_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837890.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11182..12399)
                     /locus_tag="IW656_002198"
     CDS             complement(11182..12399)
                     /locus_tag="IW656_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0837891.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12782..12865
                     /locus_tag="IW656_002199"
     tRNA            12782..12865
                     /locus_tag="IW656_002199"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12816..12818,aa:Leu,seq:gag)
     gene            complement(13105..>13694)
                     /locus_tag="IW656_002200"
     CDS             complement(13105..>13694)
                     /locus_tag="IW656_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837892.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4142 a         2793 c         2365 g         4394 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccaatcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaaag
     8761 cttttatttt gctaatacgt attaacattt ttaattttat ctttacattg ttacttattg
     8821 aacaatagga aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca
     8881 aaaaaaacct taaaaccaaa cttaagaagt ttagttttaa ggttaactta aattatatta
     8941 tgcgtggaaa cgttccacgc catctagata agttgcttcc aatgtcatat cagggttcaa
     9001 tacgataaag tctgcatcgc gtccttgagc gatttttcca catgtatcat cgattttaca
     9061 gctgattgct ggaatcagac tagccatcat gactgcttgt tctggtgttg ctaatcccca
     9121 gtctacaaca tttttaattg cttctttcaa tttcaatata cttccagcta agttgccaga
     9181 ttctagacgt gctgttcctt cttttactac tactggaaat tcgcctaaat tgtaatttcc
     9241 atcaggcatc ccaccggcca tcatacagtc tgtaattaat gcgacatgat catgtcctgc
     9301 tttttccatt aggattcccg cagcatttgg atgtacatga tggccatcac aaatcaattc
     9361 agaaaagaca tgttctaaag ataataacgc acccaccata cctggttcgc gatgattcaa
     9421 tccacgcata ccattgaaag catggacaaa tacacttgcg cctgcttcaa cagctttttg
     9481 cgcttcttct agtgtagcat tactgtgtcc tagagaaaca actactcctt cttcagtcac
     9541 ttgtttgaca aactcttcta caccttgacg ttccggtgct aaagcaattt ttttgattaa
     9601 accacctgaa gcttcttgcc attcatggaa agtatccaaa tcaggatccc cgaaataact
     9661 tgggttttgt gcccctttgt gttcttctgt gaagaaagga ccttcaaaat agattccttt
     9721 gattttagct cctggtgctt cctgatacat tttgccgatt gtctcagcta catcttttaa
     9781 acgttctttg cttgacgtca atgttgttgg taaaaatgaa gtcactccac aagataacag
     9841 accttcagac atcactttga tgccttctgc atcattatcc attacatcat gattcatata
     9901 gccgtgaata tgtgtatcta ccaaaccagg agcaatccat ttgccgcttt ggtcaatgat
     9961 gttgacttct ccctcaggta gttctttggt gtattcacca aaaagtccat cggtaatttc
    10021 aagatagcct gcatgtttga cacctgacgc taaaaagaat ttgtcagcat agataaaagt
    10081 tctcattcta ttctctccct ttgtttttct acctctaaag tactcttatt tagagaagaa
    10141 gtcaagaatg gtctacacca tttacaaatt ttttgaaatt tgtttcacag cttgtgctaa
    10201 gccttcatag aatgctgctc tttctggttc ttctttacgc ttgtatatta agcttttaag
    10261 cacaaataaa tcattgaggt aatagtagct attcttctct tgagcccaat agattccttg
    10321 attcacctgt tctaacgctt cgtcgatttc attttgatgg aataagaaat ttccataatt
    10381 ataaaaaatt tgcgaaaatt cactttttga tcgttcatca gctgattgat gcagcagttg
    10441 gatgcttcga ctcaaatagt agcgtgcttc agccgttttt cctgccacac catatatttt
    10501 tccgatacaa ctaatcagtt gtatttctgt gtcagaaaca tggattcttt ctttttggct
    10561 agagtagctt aatgcttctt tcaaataata aagggactga gagatatttt gacgcaacgt
    10621 gaattcgcaa acaccgagat aataataata gcgttgaaag tcagttgctt gatacagatt
    10681 ttcagaaata tcagaagatt ttagtacttg ttctaacttc cgataatcac gtttttggaa
    10741 acaactatcc aacagttcaa ataatttatc gtttgtccga gtagtttttc gagagagatt
    10801 catcacatga tcaatcgtta cccctagccg ttgacaaatc tgcatcatca ccaatacatt
    10861 cggcagttcc tcattatttt cgatccgact taatacactt tgtgaacaaa tatcatgcga
    10921 aagcattttt tgcgtcattt tttgttcttt tcgaatctct ttaatgatcg ttccaaagga
    10981 ctcgattaac gtttccataa gtatcctccc caaaatatgc gtattcgcat tctattcgta
    11041 ttttacttta ggaagtcgca aacttcaaga aaagacttgc aactaaataa tatactttag
    11101 ttatttaaga aaactccact agaaagagag cctcagtgtg tttgtctgtg gccctcctac
    11161 tgaaaaattt ggtttttaaa atcatttttg gcgataggtt gctaggaatt ctctcatttt
    11221 cttagctgtt tgtttcaaat catccatttt aggaagataa acgattcgaa aatgatctgg
    11281 ctggttccaa ttaaatccac caccatgtac aagtagaata tgatgttcat gtaaaaagtc
    11341 caaaacgaat ttttcatcat ccaaaatatt gaatcgcttt gtatcaattt ttgggaatat
    11401 ataaaaagca gcttttggtt ttacggctga aagaccagga atatcattga ttgcattata
    11461 gatatattct ctttgttcat agatccttcc gcctggcagg agcagctcat cgacactttg
    11521 ataaccgcct agcgcggttt ggataatttg ctgagacaat acattggaac acaaacgcat
    11581 ggaggacaac atattcagcc cttctatata gcctttgacg tgtgacttat ctccacttag
    11641 gaccatccag cctactcgaa atcctgctac acgatgagat ttggatagac cgttcagcgt
    11701 aacaacgaac aaatcaggcg ctaatgtagc aatcggaata tgttctagac catccatcac
    11761 taagcgatca tagatttcgt cggaataaat aatcaggtta ttttgtcgag ctacttcaac
    11821 gatttcttct aataattctt ttggataaag tgccccagta ggattattag gattaatgat
    11881 aacaatagct tttgtccgac tcgttatttt tgatttgata tcatcaatat caggatacca
    11941 ttctgcctct tcatcgcaaa tataatgaac aggtgtgcct ccagctaatg caatagaagc
    12001 cgtccacaac ggatagtccg gcattggaac cagtacttca tctccattgt ccagcaaccc
    12061 ctgcatacac atcgtgatca attcgctcac tccgttgccg gtgtagatat cattgatcgt
    12121 cacattcgga aaatgtttca gttggcaata ttgctcaatt gcttttctag cagaaaagat
    12181 ccccttggaa tctgagtagc cttctgagtc acgggcattc atgatcaaat cacgaatgac
    12241 ttcgtttggt gcttcaaatc caaaaggtgc gggatttccc gtgttcaatt ttaaaatact
    12301 aatgccctct tcttgcatcc gctccgcttc ttctagaacg ggaccgcgaa catcgtaact
    12361 gacaccttct aatttgttgg atttttcaaa ttctctcatc acttagccaa tccctcattt
    12421 caaaaagtta gaactttcca aattttttgt tatttactat ttcaatatca tactccatcc
    12481 tagaaaattt tgccacttat ttaggaaaaa cgtaagtatt aaaaaaacac aaacaagcgt
    12541 tgagaactta attgtcatga tagtcattcc atagattcgc tgcccaatcc attgcaattc
    12601 cacaaatgat tcctagcata aaaccaagca aaaaacgtcc gaatgcaagt ccaatcaata
    12661 cacctaagat cattcccagt cctgaaaatt ctccgtcata tttctccatc ttaataagtc
    12721 ctcccatttt tgagcttgac aactatgtaa aacctcgcta tcattagtgt gttattatta
    12781 cgcagatgtg ttggaattgg cagacaagca tgattgaggg tcatgtgagc gttagctcgt
    12841 gtgggttcga gtcccatcat ctgcactaca actcgtgaag ttaattttgc ttcacgagtt
    12901 tttttgtttg aacgtttctt tatgccatta tatacatatc actgtatcct ttacaaaaag
    12961 aacgtacaat catgtaaaca gcctgaggtg agtatatgaa ggtcggaaac aagctactcg
    13021 tagaaacttg caagtgttaa aacaattaat cgaaaaacga gtaaaatccc tcacctaata
    13081 ctaaagcgag ggattttttg tatattatct gtttatcacc gttattataa aggtacaaat
    13141 accaagcacg gttaatccaa attgcataaa ttcatttttt tgtacactca tgcttttcat
    13201 tagctcttta actgccatta gtactaagat ggcgataaca acataaagta tatagtacgt
    13261 ccgcattccg gaagcccata ctgtgggaga aaatcccata attaaccttg aacaaaatcc
    13321 cattatcatc aaaataacta aaaaaataga agttttctta ttgtcaaaac ttaaataaat
    13381 tccaacaatt aaacatagta ataatgccaa gactactaaa gtagctatcc aagtacctgg
    13441 ataaaaaagt gacagtttcg ttcctaactc tgtaaagaga ttattcaggt tactactact
    13501 ccaaatcatt gcccttttat ttccattgac gtaagtaaag ctttgaccca ttgtattacc
    13561 aaaataaata atcaggttta agaaaaacgg taatgcagtc aatatacgca cataatagtt
    13621 ttgacatttc ctatatgtta gaaggaaaat taagaaaaat agcaataaaa ataagatatt
    13681 catgtctaaa aaaa
//
LOCUS       DACEJZ010000081        13212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEJZ010000081 DACEJZ010000000
VERSION     DACEJZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.084"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW656_002201"
     CDS             147..1016
                     /locus_tag="IW656_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0837893.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW656_002202"
     CDS             1225..2250
                     /locus_tag="IW656_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0837894.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW656_002203"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW656_002203"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0837895.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IW656_002204"
     CDS             3333..5036
                     /locus_tag="IW656_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837896.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW656_002205"
     CDS             5038..7071
                     /locus_tag="IW656_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0837897.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW656_002206"
     CDS             7082..7522
                     /locus_tag="IW656_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0837898.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW656_002207"
     CDS             complement(7722..8849)
                     /locus_tag="IW656_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0837899.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW656_002208"
     CDS             complement(8852..9529)
                     /locus_tag="IW656_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0837900.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW656_002209"
     CDS             9793..11559
                     /locus_tag="IW656_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0837901.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW656_002210"
     CDS             11574..12011
                     /locus_tag="IW656_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0837902.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW656_002211"
     CDS             12024..13175
                     /locus_tag="IW656_002211"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0837903.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DACEJZ010000082        13145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEJZ010000082 DACEJZ010000000
VERSION     DACEJZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.085"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..149)
                     /locus_tag="IW656_002212"
     tRNA            complement(77..149)
                     /locus_tag="IW656_002212"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IW656_002213"
     CDS             530..1099
                     /locus_tag="IW656_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0837904.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IW656_002214"
     CDS             1086..1946
                     /locus_tag="IW656_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0837905.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IW656_002215"
     CDS             2136..2840
                     /locus_tag="IW656_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0837906.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IW656_002216"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IW656_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0837907.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IW656_002217"
     CDS             4677..5993
                     /locus_tag="IW656_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837908.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IW656_002218"
     CDS             5994..6863
                     /locus_tag="IW656_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837909.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IW656_002219"
     CDS             6918..7727
                     /locus_tag="IW656_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0837910.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IW656_002220"
     CDS             complement(7830..8459)
                     /locus_tag="IW656_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ0837911.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IW656_002221"
     CDS             8629..9921
                     /locus_tag="IW656_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0837912.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IW656_002222"
     CDS             complement(9997..10659)
                     /locus_tag="IW656_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0837913.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IW656_002223"
     CDS             10884..11168
                     /locus_tag="IW656_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0837914.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IW656_002224"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IW656_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0837915.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DACEJZ010000083        13006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEJZ010000083 DACEJZ010000000
VERSION     DACEJZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.086"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW656_002225"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW656_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0837916.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="IW656_002226"
     CDS             complement(1258..3099)
                     /locus_tag="IW656_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0837917.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5674)
                     /locus_tag="IW656_002227"
     CDS             complement(3281..5674)
                     /locus_tag="IW656_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0837918.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="IW656_002228"
     CDS             complement(5781..6641)
                     /locus_tag="IW656_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0837919.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="IW656_002229"
     CDS             complement(6700..7221)
                     /locus_tag="IW656_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0837920.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7541..8260
                     /locus_tag="IW656_002230"
     CDS             7541..8260
                     /locus_tag="IW656_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0837921.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8286..10877
                     /gene="mprF"
                     /locus_tag="IW656_002231"
     CDS             8286..10877
                     /gene="mprF"
                     /locus_tag="IW656_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0837922.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW656_002232"
     CDS             complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW656_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0837923.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12620..12976
                     /locus_tag="IW656_002233"
     CDS             12620..12976
                     /locus_tag="IW656_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0837924.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3738 a         2718 c         2310 g         4240 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8581 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8641 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8701 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8761 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8821 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8881 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8941 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     9001 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9061 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9121 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9181 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9241 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9301 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9361 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9421 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9481 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9541 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9601 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9661 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9721 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9781 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9841 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9901 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9961 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    10021 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10081 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10141 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10201 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10261 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10321 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10381 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10441 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10501 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10561 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10621 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10681 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10741 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10801 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10861 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10921 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10981 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    11041 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11101 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11161 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11221 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11281 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11341 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11401 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11461 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11521 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11581 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11641 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11701 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11761 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11821 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11881 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11941 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    12001 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12061 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12121 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12181 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12241 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12301 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12361 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12421 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12481 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12541 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12601 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12661 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12721 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12781 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12841 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12901 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12961 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaa
//
LOCUS       DACEJZ010000084        12705 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEJZ010000084 DACEJZ010000000
VERSION     DACEJZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.087"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW656_002234"
     CDS             6..305
                     /locus_tag="IW656_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0837925.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW656_002235"
     CDS             378..1739
                     /locus_tag="IW656_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0837926.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW656_002236"
     CDS             1761..2021
                     /locus_tag="IW656_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837927.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW656_002237"
     CDS             2035..2787
                     /locus_tag="IW656_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0837928.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW656_002238"
     CDS             complement(2881..3486)
                     /locus_tag="IW656_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0837929.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW656_002239"
     CDS             complement(3539..4513)
                     /locus_tag="IW656_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0837930.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW656_002240"
     CDS             complement(4891..5670)
                     /locus_tag="IW656_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0837931.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW656_002241"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW656_002241"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0837932.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW656_002242"
     CDS             7161..8558
                     /locus_tag="IW656_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0837933.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW656_002243"
     CDS             8639..9079
                     /locus_tag="IW656_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0837934.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW656_002244"
     CDS             9227..9553
                     /locus_tag="IW656_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0837935.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW656_002245"
     CDS             9554..10303
                     /locus_tag="IW656_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837936.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW656_002246"
     CDS             complement(10383..10838)
                     /locus_tag="IW656_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0837937.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW656_002247"
     CDS             complement(11265..11540)
                     /locus_tag="IW656_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837938.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW656_002248"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW656_002248"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0837939.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4069 a         2184 c         2592 g         3860 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaa
//
LOCUS       DACEJZ010000085        12246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEJZ010000085 DACEJZ010000000
VERSION     DACEJZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.088"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW656_002249"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW656_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0837940.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW656_002250"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW656_002250"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0837941.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW656_002251"
     CDS             complement(2169..2906)
                     /locus_tag="IW656_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0837942.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW656_002252"
     CDS             complement(2997..3524)
                     /locus_tag="IW656_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0837943.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW656_002253"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW656_002253"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0837944.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW656_002254"
     CDS             complement(4401..4886)
                     /locus_tag="IW656_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0837945.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW656_002255"
     CDS             complement(5039..5236)
                     /locus_tag="IW656_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837946.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW656_002256"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW656_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0837947.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW656_002257"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW656_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0837948.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW656_002258"
     CDS             9211..9423
                     /locus_tag="IW656_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0837949.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW656_002259"
     CDS             9425..11104
                     /locus_tag="IW656_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0837950.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW656_002260"
     CDS             complement(11358..11558)
                     /locus_tag="IW656_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0837951.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW656_002261"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW656_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3575 a         2636 c         2117 g         3918 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaat
//
LOCUS       DACEJZ010000086        12187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEJZ010000086 DACEJZ010000000
VERSION     DACEJZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.089"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            597..1925
                     /locus_tag="IW656_002262"
     CDS             597..1925
                     /locus_tag="IW656_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0837952.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="IW656_002263"
     CDS             2127..2960
                     /locus_tag="IW656_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0837953.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="IW656_002264"
     CDS             2976..3713
                     /locus_tag="IW656_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0837954.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4836)
                     /locus_tag="IW656_002265"
     CDS             complement(3868..4836)
                     /locus_tag="IW656_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0837955.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5068..5901
                     /locus_tag="IW656_002266"
     CDS             5068..5901
                     /locus_tag="IW656_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0837956.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5935..6774
                     /locus_tag="IW656_002267"
     CDS             5935..6774
                     /locus_tag="IW656_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0837957.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6800..7360
                     /locus_tag="IW656_002268"
     CDS             6800..7360
                     /locus_tag="IW656_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0837958.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7559..9280
                     /locus_tag="IW656_002269"
     CDS             7559..9280
                     /locus_tag="IW656_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0837959.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9445..10587
                     /locus_tag="IW656_002270"
     CDS             9445..10587
                     /locus_tag="IW656_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0837960.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10603..11814
                     /gene="thiI"
                     /locus_tag="IW656_002271"
     CDS             10603..11814
                     /gene="thiI"
                     /locus_tag="IW656_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0837961.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4156 a         2043 c         2530 g         3458 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4861 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4921 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     4981 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5041 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5101 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5161 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5221 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5281 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5341 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5401 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5461 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5521 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5581 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5641 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5701 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5761 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5821 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5881 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     5941 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6001 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6061 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6121 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6181 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6241 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6301 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6361 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6421 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6481 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6541 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6601 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6661 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6721 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6781 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6841 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6901 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     6961 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7021 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7081 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7141 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7201 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7261 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7321 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7381 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7441 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7501 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7561 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7621 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7681 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7741 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7801 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7861 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7921 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     7981 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8041 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8101 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8161 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8221 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8281 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8341 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8401 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8461 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8521 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8581 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8641 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8701 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8761 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8821 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8881 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     8941 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9001 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9061 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9121 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9181 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9241 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9301 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9361 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9421 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9481 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9541 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9601 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9661 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9721 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9781 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9841 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9901 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
     9961 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10021 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10081 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10141 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10201 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10261 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10321 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10381 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10441 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10501 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10561 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10621 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10681 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10741 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10801 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10861 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10921 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    10981 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11041 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11101 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11161 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11221 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11281 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11341 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11401 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11461 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11521 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11581 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11641 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11701 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11761 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11821 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11881 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    11941 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12001 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12061 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    12121 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgtgaa
    12181 aaagctg
//
LOCUS       DACEJZ010000087        11814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEJZ010000087 DACEJZ010000000
VERSION     DACEJZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.090"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(218..1015)
                     /locus_tag="IW656_002272"
     CDS             complement(218..1015)
                     /locus_tag="IW656_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0837962.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IW656_002273"
     CDS             complement(1012..2196)
                     /locus_tag="IW656_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0837963.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IW656_002274"
     CDS             complement(2221..3468)
                     /locus_tag="IW656_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0837964.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IW656_002275"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IW656_002275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0837965.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IW656_002276"
     CDS             5448..5762
                     /locus_tag="IW656_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0837966.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IW656_002277"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IW656_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0837967.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IW656_002278"
     CDS             6447..7091
                     /locus_tag="IW656_002278"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0837968.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IW656_002279"
     CDS             7106..7435
                     /locus_tag="IW656_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837969.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IW656_002280"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IW656_002280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0837970.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IW656_002281"
     CDS             8423..9247
                     /locus_tag="IW656_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0837971.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IW656_002282"
     CDS             9240..9587
                     /locus_tag="IW656_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0837972.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IW656_002283"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IW656_002283"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0837973.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IW656_002284"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IW656_002284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0837974.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
BASE COUNT         3777 a         2211 c         2556 g         3270 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 ttttaaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt ttta
//
LOCUS       DACEJZ010000088        11605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEJZ010000088 DACEJZ010000000
VERSION     DACEJZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.091"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            418..624
                     /locus_tag="IW656_002285"
     CDS             418..624
                     /locus_tag="IW656_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0837975.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(826..1827)
                     /locus_tag="IW656_002286"
     CDS             complement(826..1827)
                     /locus_tag="IW656_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0837976.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1832..3745)
                     /locus_tag="IW656_002287"
     CDS             complement(1832..3745)
                     /locus_tag="IW656_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0837977.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3913..4419)
                     /locus_tag="IW656_002288"
     CDS             complement(3913..4419)
                     /locus_tag="IW656_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0837978.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4579..5019
                     /locus_tag="IW656_002289"
     CDS             4579..5019
                     /locus_tag="IW656_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0837979.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5045..6202)
                     /locus_tag="IW656_002290"
     CDS             complement(5045..6202)
                     /locus_tag="IW656_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837980.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6205..6561)
                     /locus_tag="IW656_002291"
     CDS             complement(6205..6561)
                     /locus_tag="IW656_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837981.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6859..7833)
                     /gene="sgrA"
                     /locus_tag="IW656_002292"
     CDS             complement(6859..7833)
                     /gene="sgrA"
                     /locus_tag="IW656_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0837982.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8029..8868)
                     /locus_tag="IW656_002293"
     CDS             complement(8029..8868)
                     /locus_tag="IW656_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0837983.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9053..9940
                     /locus_tag="IW656_002294"
     CDS             9053..9940
                     /locus_tag="IW656_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0837984.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10524..11219)
                     /locus_tag="IW656_002295"
     CDS             complement(10524..11219)
                     /locus_tag="IW656_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0837985.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11203..11601)
                     /locus_tag="IW656_002296"
     CDS             complement(11203..11601)
                     /locus_tag="IW656_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0837986.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3457 a         2307 c         1735 g         4106 t
ORIGIN      
        1 tttattattt ttgtttctta ttttctaaaa aattaaaaaa aattttggtg aattaagtat
       61 ttccttgata acactggctt ttggcctcat tttaggttat catttgtaat tttatttcac
      121 agttgatatg attgttatgt aaaataaaaa agtttaggat acatcgaatt tttggggaaa
      181 ttcttttggg ggtatcctaa atcttttcta ttatattccc atattttcta ttattggtct
      241 agacgtatga tggtaaactt tgtacctatc tgattgctga atcattaatt gcttatcatt
      301 agccatcttt gtttcaagtt cgctagatga tcattttatt cacatcctaa caaacttgtc
      361 acatttatag attcttaatc tatttatcat acaattataa aaaaggaggt gctgcttatg
      421 aaagctatcc atatttcttt gctttttttc gctagtgcat tgattttatt tgctattcat
      481 ctttgttccc agccttatat caataacaac ggaatattag tggaaccagc attcttttgc
      541 ttaccactcg gctttttgtc ccttcttgcc tccgcaggct tcggatttgt tgcttttttt
      601 aaacgatcaa aacaacaaat ctgacttata aaatttacgt aaaagagatc gtgacaataa
      661 tcgtgtgata ttcccaaatt tcgaataaaa ggtgcccctt gccatctcta actcctcagt
      721 cacaagttac aatattcata aaccatgaaa agcttgttta ttgaaattgc tccttgtgac
      781 ttggagctaa gtgacagatt acaacctctt ttgcatcata agactttact ccttgaaaaa
      841 cattactaac ttttgatatg tttcttgcgc ttgtttattg ctgaaatcaa actgatattc
      901 atggttcaaa acagcatttt tctccgcata atctactttt gtgactttca cccccgcttt
      961 ctcaaataca gaagttactt cttccatttg tttcgtaaac gtatttgtat ttccatccgt
     1021 taagaatgtg ttttgcgtcc aacgatccgc atgtctgacc aaggctaaac ttttgacctt
     1081 ctctgattgt tcaaagtcct tattatcaaa gtaagcccat ccacattttt cataaagaaa
     1141 gctagagatt ttatcactta cttgagtaag tgcagaagga tccaacagac cagacaaaga
     1201 aataaacttc gtaatctgct tgttctccaa agtcggatca agatgatcag cttttttaat
     1261 ttcttcattt tgaagtgcta acaaatattc tccactaatt tgtgcacctg ccgaatttcc
     1321 accaatcgaa atattcgacc aatctgcttg atcttttatc tcttttttag ctgcttgaat
     1381 ggcttcgttc aattggataa ctggtgtagg atgttttgct tccggtgatc gttcataatc
     1441 tatatttacg atgatttgct gcgtgtctga acatagatat tccaaataat cacgacaatc
     1501 atttttatcc cctcctacat atgcgcctcc atgcatccag aaaagaatcg gtaatttttt
     1561 cttttccact ttcggtgtgt agatatccat attattattt ttgtactttg aaggatagga
     1621 tatttctttt tttatatcga tttgttcgat tttttgctgg aaatcatctg gtcgggcata
     1681 tacttgttct ttttcacttg tgtcaaattg ttttctaaca agcaagctaa tcggctttgg
     1741 tgtgatttga ttaatcaaaa atacagtgac taaaataact acgatgataa ctggtactgc
     1801 aatcactatt cgcttgaacc atttcattca tttaccctct ctttcttcgc caaatccatg
     1861 tgataatcgt gctaacagat aaaaatccac ctgaataaat gagaagcgaa tgtttctttt
     1921 ctccagtttt cggcaacatt cttttttttg catctgtctt tttttgtact ttcactgtca
     1981 tctgaatcgg ttgcgtatca tcacttgatt gatcctctcg aggttcattc tttcccttat
     2041 tgtctgcatt ctctgtcgga tcttgctttt ttggtggagc tactgggatt tttttgtaat
     2101 agaaaataat agttggtatc gtctcattaa aaatcccttc agtcggtcct tcaacatttg
     2161 ctaattcgta tccttcgaat gtcttttgct ctgtcatata attttctcca taagctccgt
     2221 aaagctggat ttcgtcctgg tctttgagta gacttttatt gttttcatcc acatacttta
     2281 cgtttatctg atcatggaac actactttca aaaagtccgt tacatgtttc gtgtaggcag
     2341 catggtcaac ttcctgtgcc tttccatgcc ctgctccttc tactaataaa agatctttat
     2401 accctcctga taactcatat aatttttgcg cgttagacac aggtactaca tcatctgctg
     2461 ttccatggat aatcaattta ggcaatgaat ctgacattcc aatcgtaggt agagcagctt
     2521 ttgtatccaa atgcatctcc tgatcaaaat agtattgttt caaaaggccc ataaataaat
     2581 cttcatgctc ttgatcccca atgatgtcta actgattttt tatggcatcc ggtagtgcgg
     2641 gggctactgt ctggctgtac gtaaaacgta gttgttcatc aattgatgca aatccgcaat
     2701 tttcaataat tcctttgaca ttgacaggaa tatctttcgc taatacagac atcatcgtag
     2761 ctgctcccat cgaaccacca taaagtaaaa tcttttgaga tggccgctct cgaacttctt
     2821 gatttatcca gtaaaggacg tcatctgact catattgtcc ataggtcaca taatcgcctc
     2881 cgctattacc agtcgctctg gaatcaacaa ataataaatt atacccggcc ttataaaaat
     2941 cattgtattc ttctgtaacg atcccgttat tccaattccc tcgaaatccg ccatggatca
     3001 tgactgtttt atctgaacct tgatcgacat aatatgcata tacatccatc attttgccat
     3061 cttgtttgtt tagtacagtt tgatgaagtt gttttgtatt tggatagaag cctgtgtttt
     3121 ctgtaggcgt tgtcccatca tctgtacctc caagcatcga catcaacaca ctgttttttg
     3181 caaaagcgat atcgtacaat agataagtag caatcagatg tttgataatc tctttatcta
     3241 caccataatc attaacagca agctcagtga ctaataactc tattaatttt gacttgatgt
     3301 cttctgttgg tccattaagt atttctgtca tctgtttacc gaagtaattc aaatattctt
     3361 taccattgaa attttcacct ttaccgatat catcattttc tgtcccattc tctttgtcac
     3421 ttactttagc agaagaggat tcgtcgtttg acatttttgt agctgttgct tcttctgttt
     3481 gttggttatc tgtggtagcg gtcgtttctt gatctgtgct agtaacagat gtctcaaccg
     3541 ttgtttcagt atttgtttct gtttgtgaag tactcgattt cgtttcattt ccagcatcag
     3601 tagtttctgt ggtttctacc tcccgagatt ccgattcgga agaaatgctc tctccatata
     3661 ctgtccattg cgggacgctt gtacttacag caaataagat gaccaacgct ccctttaata
     3721 cttgttttgt tctcaagcta ttcactcctt tttatgtttc taggagaatt atatgtaaac
     3781 gcaaacattt tttatacaaa aaataaatgg tttatcccat aaaaatagaa caggattatt
     3841 ttctcatttt acgtaataaa gcagaatgga taaaccagta tccattctgc tttttttaaa
     3901 cgaagaaaca acttattgct ccaaaaatgt cagaaatcgt ttatcccaaa ttttctcgta
     3961 aaaagcgatt gtttcctttg ctttcataaa aggattatca taagttgtcg ttgctcctct
     4021 acacatcata agctggtatc ctaatccatg agcaaacttc acagtcgtat ctatacaata
     4081 ctctgcttgt gccccacata attcgatcga atgacagtcc aattcagtta ataaaacact
     4141 caaattagtc ttataaaatg aattcgcatg ggtttttctc acaaaataac tgtgctcagg
     4201 aatagataat tccgacaata gctcccatgg ttttgtttgt ggcactaatt gagcatcttc
     4261 gtgttgaacg aaaataactg gtttcccaat cgtttcgtat gttttgattc ttttattcac
     4321 taatgctgtc actgtttcta attcgtgtat cggcgaagaa tcaaaacata cccctttttg
     4381 taaatcgatc actactattg cgtcggctgt gtttgacata cttgtgcctt ctttcttttt
     4441 tcttgattta atagtatttc ttatctagct agttccatta tacgtgacaa cattttctta
     4501 ttttacacct ctaaatggca cttttcgact atttcttcat gttattattt cataaatact
     4561 tagctattgg aggaatgtat gatgattaaa ttgtatacag gttctggctg ctcgtcttca
     4621 agacgcgcaa aacgttggct tatcgtccat gatataccgt ttatcgaaca gaacttaatt
     4681 cgtcaaccac taaccaaaaa tgaatttatc cacttactaa aattgacaaa caacggcgtg
     4741 accgaaatgc tctctactca ttctattagt tataaaaatc ttgttcacag aatagatgaa
     4801 ctcagtttga gtgaactata tattgagatt cgtacatccc cttctttatt gaaaaaaccg
     4861 attatctacg accaatctgc aaaacttttg actggattta gcgaagataa aatcaggatg
     4921 ttcctttcta aaactaaaag aaagcaagag ctcaacgaac tcttgctttc tggtcatctt
     4981 gtcgatttta aagaaaataa tctttttgca atgtcttaat tttggcataa acaatctgct
     5041 tttcttaatt aacgaaatcc aatttttcca atatggatct atttttctca ttcctgattt
     5101 ttatgatttc agctttcaga cgcgcccaat cattttttgt aggttgtcca ttccagtaaa
     5161 atatctgtgt attcgtttga tcatatgcct ttttgtcgat aggataatcg ctaatgacaa
     5221 tatcaggtaa ctgatcagcg gatgcactat attttattgg gcgttcagtc atagataata
     5281 tccttttctt tgcccataat tcctgtgttt taccatactt tgattgaata tagaatatga
     5341 caggttgttc gtgttttata aaaatatccc tgactaacat acagtattga aaaatcaacc
     5401 gtctgttctt ttcataataa gtattaagct ctggaattct gcgagctacc ccactaaaaa
     5461 actgttgcat gattggatac atcactggaa atatatgctg aaaatctctg tcttctgctg
     5521 attttccaaa agcatccacc ttcttttttg tcccaaaaac ccttgaatac aaatggatca
     5581 ttgccaaatt gacatataga taattttttt ctgaagttgt aaaacaaatc ttgaagtttt
     5641 tttcaaaatt ttctacaata gacgattcaa tccagcccca actttcttgt actttatctg
     5701 gagtatccaa caaatcttct tccaaatagg tgttcattac accaaacata aaataaaggt
     5761 aagcttgctc tgttggagtg atgtgattca tcgaccagaa gtttgcttca aaaaaaggct
     5821 ctagtatgta atgcgtaaac tcttcttttg ttatcaatgg atgcacaaat ttgttcatct
     5881 cttctgggac atacgtaatc gagtggcctt gtttcattcg ttgcaacgaa attcttagta
     5941 ggctgatcca tttctttcta tctatactac gcgtatatgg cgcatgtttc gcaaattttt
     6001 ctaatagttg acgtttattt tttatacttg ataatatatc agaatgattt tcgtcgaatg
     6061 actgccaata catcaagtga aaaaagtatc ggatacggta ttcttctcct tctatcggat
     6121 tacctttttt caagctgcaa gataaatgat agtttttcaa aatagtatta agttctctta
     6181 attttctttg aatcgtacgc aaacttatat aattttttga ctaagatttt ctaaggattg
     6241 ataagtacct ttgtaaagtg aatccagtaa aatataacca atcgttccct ctaaaagtat
     6301 agaataaact ttactcagta aaaactgaga agaaaactcg acttttatca tcttgttttc
     6361 tgaaagaaaa gataatttat attctggata cctttcgcaa aacgattctg cttgttgaat
     6421 caccgaattc atcttagcca gggtgtagtt tagatctaaa gcgatatcct tcaccacata
     6481 taatttctcg tctctttcat aacaatagtt caaaaattga tatacttgcc tttcattgat
     6541 atcaataaat tcttcaaaca aagtacatac tcctttttat ctattattta gacttattcc
     6601 gacagctaca tgaataacgt acaaatagat tcgttactga tttttcagag atactaccat
     6661 gcgcagtgtg cattgtcaat tattcttgct cttattttcc atttgtgttc ctgttatttc
     6721 ctgtttctca aatatgttag taaacaaaaa tagatctggc aaagctacaa cttcgccagg
     6781 caatacagca ctctcgaatg aaatattcac tcaaaaatct atcgttttat tcaattttag
     6841 atctgttgct tttattcttt atttcactcg atggaagaga gaacgaaaat agcttgctcc
     6901 tacaatcaaa atgagtaaca cacccatcca tgtaattagc ttgttcaagg ttctactacc
     6961 agtttctggt aaatcgcttt ttctattatc tgtttctttc gctttttctt gatgatcaat
     7021 tccacgagaa acaaaatcag gctgttttag cacacttttt aggttgctcc ctgcatttct
     7081 cttttgttcc ttagttggta tgataggtac caaatcaagt tcattattta cttttcctgt
     7141 gctgctttcg ctgctcgtgc ttcctgtgct actctcacta ctcatgcttc ctgtgctact
     7201 ttcactactc gtactctccg tactgctttc gctgctcgtg cttcctgtgc tactttcact
     7261 actcgtactc tccgtactgc tttcgctgct cgtgttttct gtgctacttt cactgctcgt
     7321 actctctgta ctgctttcac cgctcgtgtt ttctgtactg ctttcactac ttgtgctatc
     7381 cgtactgctt ggcggtccaa caactgtcca actgcttggt ttcaaattct ccgtgatcgt
     7441 catctgtcca tcataaaaaa caatcaaatc aactttatct ttactcttga tctcatcaat
     7501 cgatagttct gcagaaatca ttattgtata ctcaatacca gcggtccatt gtacgtcttt
     7561 taaataaact tccccaataa ctttttctga acttccatta ccatcccaac catatgttag
     7621 aacagcatgt acatcactag cattcacgct tttgtttggt ttgactggaa tagtaatgac
     7681 ttgttgtgtc atgtcataaa caatccgatc agttttcaca gtaaatgaaa gattctcatt
     7741 tgcccgttca tttgcagaag tactgattcc aatcattgct gcaattatac tcagcccaag
     7801 aataacaaat aatacggtcg ctgttttctt catttctatc actctccata taattacctt
     7861 ctatttgata aaggaattat ataaaatgaa aatgatgaga acacgacagt cagttgtgaa
     7921 aatctaacaa aagatgtcgt gtattcgctt gaatttgttt actttttcgt ttaatcaatg
     7981 acagacttaa aaaagcaaga ccgatatcag ccttgctttc ttcatctttt attgataaac
     8041 agcagtgtat ttcttcatcc gctttgtttt atatgcatag agtaacaaac caacaatcaa
     8101 gactaacaag aataatgtag ttgatataga tgcttcagcc ccaaagtttc ccccagtaat
     8161 tattgtattt tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg ttccgctgac
     8221 tggtataccc aaaatagctc cttgtgtcca attccaagta atatgagcac caatagccgt
     8281 aaataaactt cctgtactgt agataagtgc gctgaaaaag caacctgcta aaaagatatt
     8341 gaaaactgcc agaaatgtca tgttaggatt caatccgtgc aaaagagcaa ataatgcagc
     8401 agatccaatc attgctatta acggaccaaa tctaagcatc tgatgctgaa aaaagccacg
     8461 gaataaaagc tcttctgatg cccccactgc aataaataaa ataaaactcg ttatcagcgg
     8521 cacaatgttt gcgtttcctg aatctaaccg atacgccccc gtcaaatata atggactagt
     8581 aataagcaga aacactccaa ttccaacaac gagacctccc gctaaatacc cccagtcttt
     8641 tcctcgaaca gaaacaccat aatcttctat tctttttcct tcgaccttac tagcaagaaa
     8701 taaagctagt aaaaaaacag ctaaatgtcc gaccgtaccg gcaattgctc caatccattc
     8761 actatttcca ataaacatat ccgcaagtgc attattgaca agaattccta aaacaaaacc
     8821 aactaagact attaaaacag atccaatcaa ccgaataatc ctgatcatct ttcatccctc
     8881 ctttatatta agtttaacaa cccttttctt tcttgtctcg tgaaaactat gtaaatgttc
     8941 tccatcatag agagaataga tttttacgaa ctaagaatat ctacagcaac cttacgaaaa
     9001 tgaaagactg tctccctaat tcttctgtta gactgttttt aataaggaaa ggatgggaaa
     9061 ttttttgaaa aaacaactac taattatgtg cctttcagca tgtgcaattt tactattctc
     9121 aggttgtagc aagtccaatg aaacgattgc aacgtttaaa ggtggaacac ttacagttca
     9181 ggatttttat gataacccaa aagttaaatc aaataatcaa acactattaa aaaaaatgat
     9241 tgtcttcaaa gcatttgatg atgtatacgg aaaagagatt tcttcaaagg aagtaacaaa
     9301 agaatataat gaacaaatca aaaaactcgg tccaaactac aaggaacaat taaaagcagt
     9361 tggccaaaca gaagagacat ataagaaact ctttaaacag atgctggctt tccaatacgg
     9421 tttgaaagcc aatgtaaaat taacagataa agatctagac actgcttgga aagaattcta
     9481 tccagaagtt agtacacaga tcattctatt ttcgactaaa gaagaagcag ataaagcaaa
     9541 aaaagaagca aatgaagggg aaaatttctc caagcttgtt caagcttatg gaaaaaataa
     9601 aacattaaag gaaactgatg gaaaaatgaa ttttgattca accaatccag agattcctac
     9661 tgaagtaaaa aaagcagcat ttaaactaaa aaacggggaa gtttctgata tcattcctgt
     9721 aacagatcca acgacttatc agcaaagcta ttatctagta aaaatggtaa aaaaacagga
     9781 taaaggttct aataaagaca aatacaaatc tgagctagaa aaaattgcta ccgaagcaaa
     9841 aactcttgat acggaattca tggataaaac aatccgtaaa gtgatgaaaa aagacaatgt
     9901 aactattaaa gatccttatg taaaaaatat attcaaataa acaaatttga cccaattggg
     9961 gatgtggcaa aatttgagaa gtgatttttg gaaattcctt cttatttttg aggcaaacca
    10021 cttttgccac aacttcatcg gggccttttt ttatttgtac caaaacaaat cactggattt
    10081 gttacaatca tgaggatata atttaaaaaa gttttttatt tctatcacat gaatcaatgc
    10141 gccattaagt agattctttc atgcttccct attttttcac ttgccgtatc gcttcaaaaa
    10201 caactatcat aaactcaaac tcgactgaaa agttatttta aaaaagacta aaaatatccc
    10261 ttctggactt gttcctctta ttctgttata ctagagaaac acgaaagata tttttatgcg
    10321 acacttttca tttgttttta tgaaatttat tcaaaactgg cgtaacttca gagttacaag
    10381 gatgtattag aggttaggga atacatcgtt taaatgagaa ataagtaata gtcacccaaa
    10441 ctttaaatta atgaatattg aatcgtcaga tagcctcctc gagttttcga gaaggctatc
    10501 gtttatttta tggagaaatt gattcagaag taatcagcga ccttttttag ttcaccaatc
    10561 gtttctgctt ggaaagtttg cacccaatct tcttggaagc ccctcactgc tttatcaatc
    10621 gagggcatct ctaatacttg atctagtagc tctttaccta acgttatcgc atgagctcca
    10681 gatcgaatgg ctgtagtgac ttgttcacaa tttttgaaac tagctgctaa tatttttgtt
    10741 ggtctttgcg tacgttcgat ctcagtccgc aacgaatcaa tcactttcat agcatcaatg
    10801 ccttgatttt gcatcctgtt ataataaggt gcaatataat ctgctccatt attaatagct
    10861 aaataccctt gtaattctgt gtaaatagct gtaccggtaa tcaaataacc ttcttttttc
    10921 aactttttga tcgcttgaag gcctgcttga ttcaccggca ccttgataaa cgtatctttt
    10981 cccagttctt ctatgataac tcgagcatcc tctatcattt cttcttccgt tcttcctatt
    11041 gtctgaatat ggataggaat ctgtccgatt atttttttga tttctttcat atgcgagaag
    11101 aagtttgacg ctccttcttt tttcagaatt gtaggatttg ttgttacgcc tgttaaattc
    11161 aaaaactgtt gacaagaata aatacttttt gtatttgctg tatcaagcat gatttccata
    11221 tagctttccc ccattctgat cctcttgatc gattttaatg attgtcccct ttcttaaatg
    11281 cggcatttcc ttatcttcta catacattgt tcctggaagt tccggctcat gagatccatc
    11341 aaattttatc gtgatatgtc ctaattcatt caaattagtt tggacaacat ctcctactgc
    11401 tgtgattaca tacatttgtt tatcaaaaca gagcttcata tcacaagtta tttcttttct
    11461 tacctgattc aattcaatct tataacaata atctgttaat gtttccggtg cttcatctcc
    11521 aaataaaatc attatttttt ctttcctgaa tacttctgct tctataccaa catctgttac
    11581 aaccgtttca aagatcgtca tctct
//
LOCUS       DACEJZ010000089        11393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEJZ010000089 DACEJZ010000000
VERSION     DACEJZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.092"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..1420)
                     /locus_tag="IW656_002297"
     CDS             complement(41..1420)
                     /locus_tag="IW656_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ0837987.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1441..2988)
                     /locus_tag="IW656_002298"
     CDS             complement(1441..2988)
                     /locus_tag="IW656_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0837988.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3237..4016)
                     /locus_tag="IW656_002299"
     CDS             complement(3237..4016)
                     /locus_tag="IW656_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0837989.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4091..4549)
                     /locus_tag="IW656_002300"
     CDS             complement(4091..4549)
                     /locus_tag="IW656_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0837990.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4561..5820)
                     /locus_tag="IW656_002301"
     CDS             complement(4561..5820)
                     /locus_tag="IW656_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ0837991.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5936..6250)
                     /locus_tag="IW656_002302"
     CDS             complement(5936..6250)
                     /locus_tag="IW656_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0837992.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6396..7892)
                     /locus_tag="IW656_002303"
     CDS             complement(6396..7892)
                     /locus_tag="IW656_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ0837993.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8070..8549)
                     /locus_tag="IW656_002304"
     CDS             complement(8070..8549)
                     /locus_tag="IW656_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ0837994.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8925..9170)
                     /locus_tag="IW656_002305"
     CDS             complement(8925..9170)
                     /locus_tag="IW656_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0837995.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9275..10048
                     /locus_tag="IW656_002306"
     CDS             9275..10048
                     /locus_tag="IW656_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837996.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10277..10525)
                     /locus_tag="IW656_002307"
     CDS             complement(10277..10525)
                     /locus_tag="IW656_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0837997.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10624..10938)
                     /locus_tag="IW656_002308"
     CDS             complement(10624..10938)
                     /locus_tag="IW656_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0837998.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3542 a         2344 c         1707 g         3800 t
ORIGIN      
        1 atcatttctt ccgttttaaa gaagaaatga ttttcataga tcaaccttta actaaagcta
       61 gattcttttc taaaaagaat ggtgtgtagt agcccacacc gctggacaac ggtaaaactt
      121 catccggcaa ctcgatgccc agttcgttac ggatgtactc ttggcgagaa atgattcgcg
      181 accaaacatt tggatattct ttggctagtt gatcacgaag cgactcatca gctagcgcaa
      241 tcccactctc acagcttgcg ccggcatagc cagcaactga tggaataata tcaatttgta
      301 gtaacattcc ggacttaaga gtaatttctg agtctttttt cattggcgaa gacatccact
      361 cttcatcagc cgtaagatgc cccggattta agtgccattt gtattccgat ttaggcaaga
      421 ctttgcctat tactgaatat agctctttac cttgcattcc tattttgata ttttccaacc
      481 atgcgaccac ggcgtcgtag tatggagctg caactttttc aatgtaatcc ttttgccctt
      541 ctggtaggtc atctgtcgtt gcaactgcat agccacttcg acttgctaat ccgcctttaa
      601 agcccgtagt aattgacatt ttatcgccta gtttaacttg tttatcagtt ggatatatat
      661 aggcgttctc aaaccgttcc ccagttgcag caattggaat gacattattt ggttggccgt
      721 acttaactaa gtggttacca atttccattt ctgtttttcc tacctctact tcatcaatag
      781 cgttcatcac acaatctgaa gccaaggatg agccaaattc ataatatgcc acttcattag
      841 cagtatttaa tgagcgtact ccgtaatcac tgtgaatgaa caggtcggtg cgattgctaa
      901 cggcatcttc ccctactaat tctacaatcg aacttacaat gaatgccggc aaatcttgga
      961 tttggtagtt atcctcttct gaagtaaaca tcttccaacc aacaagccca actttcttac
     1021 ctttactgat tttagcattg gtcaatactt cagttagtgg tttctcgtta tccatcggtt
     1081 gatccggtaa tgagaagagt ggcgtgtgga ttaattctgc ctcaattctt gaatagtcca
     1141 ccattttagt attttcattt cctagaacta agaaagcttc acccgtctga tgtaatacca
     1201 acaagctttc ttcaaagcga gtaacaaaac ctgttaaata agagaagttc ccgccatgtt
     1261 ccaaatcaca atagattacg agggtatcta tctctgactt ttccatcgct gcaagtacct
     1321 tttctttacg gaacttcatt gtttcatcag tcaattgata tattttagct tcattatctt
     1381 ttttaggtgg gttaactttt tttagagtta ccttagtcat attgtcacta ctttctgttt
     1441 ttatttacgc ttcatttcat ttttcagaga tcgagttgcg ttttccactt gtcctccata
     1501 taccacttgt aaattatttt cgttaatttt aacgattccc attgagccgg tctttttcaa
     1561 gatatgttca tcaattgttg ctgtatcttt aagaactagt ctcagccgag agatacagtt
     1621 atcgatttct tgaatattac cagcaccacc aagtccttgc aagattagtt tgccaagttc
     1681 gttttctgtc atttcttcgt cctgatcttc caacacatca tcgttggatc gaccaggagt
     1741 tttcacatca aatcgctcaa tgacatactt aaatacaaag taatatgcaa ctgcataaac
     1801 aaggcccaat gctacaacta agtaccaacg agtataagta ccttgtaaaa ttccgaagat
     1861 agccaaatca ataaatccac caccggcatt cccaactgcg actccgaata gattcattaa
     1921 cataaaggat aaaccagcca tgaatgcatg gaagacccat agaatcggtg agataaagat
     1981 aaaggcgaat tcaattggct cagtaatccc ggttacaaaa caagtagccg ctgctgcaat
     2041 caacatccct ttaatctttg gacgttgcgc caaatatgaa gttttgtaca ttgcatagca
     2101 tgctgccggc aatccgaaga ccataaatgg atgagtacct tgtgtcaaga agcgagtagc
     2161 acttctcaaa atatccatgt cttttactgg caatgctaat tcagtattga agatggttaa
     2221 tgctccagaa actgtttcac cattgatagt tgccgtaccg ccgattggtg taaatcgaat
     2281 caactggttt agtacatggt gaagacctgt tggaatcagc aggcgctctg agaagccata
     2341 taaaaaagtt ccaattacgc ctgacttacc aatcaaaccg cctaaagcat tgatagctgt
     2401 attgacaaaa ggccagatga aaaatgaagc gattgagatg attggaatag ttaggatgat
     2461 cataattgga acaaaacgat tgccactaaa gaaactgaaa gctgctggta attgttttgt
     2521 gtgatagcga ttgtggatca tggctgtcca taatcctgca atgataccgc cgaaaacacc
     2581 catacgatat gtgaatgtgc caagtaaagt ttcatacata ttggaagttt ctactgcttt
     2641 gatttgactc attccgtcag acatcaatgt ttcaacagaa gtgctttccg ctgtgatgcc
     2701 tctaagctga agcagataat tgataactac atggaacaga ataaatgcaa taaccactga
     2761 aaatgctgca atttccttat cttctctaga catcccgatt gctactgaaa tagcaaatag
     2821 aataggaagt agtccaaaca tcgtaccaac aatagcctgt actccaccaa caaatccttg
     2881 gatcacggtt gcattagcga agctttctcc caccaaagta ggattttgta aaattgaaac
     2941 gattcctagt agtagtcccg atgcgacaat aatagacaac ggacccatta atgatttccc
     3001 taacttctgg atataagttc tcaatataaa caccttcttc tttttagttg atataaacgt
     3061 ttacatccct tgtacaacga aagtatagca ccctgaaaaa gcgctaacaa tacgataggt
     3121 ctgataaaaa tcaacattca gcacttggtt gttcattcaa aacgtgaatc ttcaatcaca
     3181 gaaaaatacg cgctatcaag cacgtatttt aggtgtaaat atatgcatca actttattaa
     3241 tcaacgctac tttgtttgta atagttaata tacccttcat acagtaagct aagagcaaga
     3301 ttcaaggtgc agaatgatga gttgttcaac tcattcacat cattaatgtt ggtcacttga
     3361 tagtataggc tatagtctga cagactggct aagttatttt gaccaaatcg tgtaactgag
     3421 accaactgtg tccctttcat atggattctt cttaaagtat catcaatccg atcaacacgt
     3481 ccggacaatg atatgacaaa tagtaaatca ttcgaggtca aagcttttga aatcaggtct
     3541 aattcattct tttccggtac aacgataatg tagattccca agttccacat gcagcgtgca
     3601 aactcattga gcatcattct ttgtccatat cctgttgcgt aggcaaatat cttatctgcc
     3661 tttctaatcg tttcatatat atcctcaaca cttttatcat cttcaaacaa tttggttgtc
     3721 gcttgaacat ctccgagtaa tacttccggt aaaaacaaac tacttttatc gactctggtt
     3781 ttttgcatgc gtgcaaattc ctctttaagg taatatcgaa attcgccata tcctttgaat
     3841 ccgagtttct tagttagtct aataatcgtt gagtcagaca catttgcttg ttttgctaat
     3901 tccgatacag tttgactgat gctgatttcc atattttgag tgataaaact taatacataa
     3961 tcatcattct cattcaattt actgtgatgt tcatttacta attgctccaa tgtcataatc
     4021 agacctccaa aaataataaa aaagattgct ctctcatttt aatacgaaag cacaatcttt
     4081 ttaagtacta ttaaattttg aaaagaagtt ctccttcaga aacctttttt tcagagactt
     4141 ctagtttaat acccttatct tcgggaaata gaaccataac aacatctgac agtttttccg
     4201 ccttgatttg gtcacaatca aaaatcacca atgactcgcc ggatgatact cgttgacctt
     4261 cttcaacgag cacttgaaac ccgactccct ttaactgaac agtatcaatt ccaatatgaa
     4321 tcaataaatc tctaccttga ttactagtca atgtgagtgc atgctttgtt ggaaatatcg
     4381 atttaatgac accgtcaatc ggtgctacca ccttagcgtt tgttggttca acagcgaatc
     4441 cttcgcccat catcccttgc gaaaatacag gatcgcttac ttggctaagg ggaatcagat
     4501 ttcctgatac tggagcaact aatttttctt ttcgactaaa aaatcccatt gacttactcc
     4561 ttaatctttt gaaatgtcca tcacaaggtt taaaagaaca tccgcccctt ttttgacatc
     4621 ctcaaaatca gtccactcat caggatgatg tgtaatgcca cctttggatg gaatataaat
     4681 catatttgtt gggacaaagt cggtcatcat catcgcatca tgtccagtac cattgttaat
     4741 gacttgatag ctatagccca actgatcgca aatatgtttc atcttctcaa cattaccttg
     4801 atccataaca cttggatttg taggattact gtagttaatt cgagccattt cctccagctc
     4861 agttctaact ccagtttccc cttgaatcac tgataactca ttcaacaact tttctttaat
     4921 ctcttgagga ttagttgtct ttgtatcaaa ggttctgatt tcgattaaag cttcgacaaa
     4981 gtcagggaca aattgatttg agttcggttt gattgtaagt ttaccgacgg ttccggcaat
     5041 cccttcacca agattcttca tccactgttc aacaaaaaga acaaatttag aagcagcaac
     5101 taaagcatct ttccgtttat tcattggagc agtcgagtcc tcagtttctc cataaaaacg
     5161 tgctttaaaa cggccgatgc caggaataaa ttcaatcaat ccgatatctt tgccttcatc
     5221 ttccaggact ggtccttgtt cgatatgaag ttcaataaaa tttttgattg tttttggatc
     5281 tcgcttagcg gcctctaaat ctggttccac tccataagca cgaagagctt ccaattttgt
     5341 ttgtccatgg cgatttttta ctgtttccaa ctcactcatg gttaatgttc ccatcatagc
     5401 tcgtgaattt gagacacccg tacttggccc aaatgtctcg ccctcctctg cattcatcag
     5461 aattaggtca attgggtaat cattttcaaa attattttct tccagaatcc gcattgtttc
     5521 cagtgcagta acaacgccag ccactccgtc aaatctgcct ccattaacaa ctgaatcata
     5581 atgagagcca atcatcactg atggtgcatc agccttttta ccggctctac gaccaaataa
     5641 tgtagagaag ccgtcttccc aaatctctaa acccagttgt tccatttgat tgatcaaata
     5701 atttcttgct tcacgatctt ctttagagta agcagagcgg gtcactcctt cacctggagt
     5761 tgcattgtac tcggctaaat tttcgatatc cttttttata cgctcaaaat ttgtcttcat
     5821 aattaaatcc tccatttcct acaagtgaat gttagcaccc atagaaagcg gctacaatat
     5881 aatcaatgaa gattgaatcg atattcgaaa actgaatatt gcttcggtta atgacttaga
     5941 aatcattatc gtagattcca ccataagaac tttttctcgt ctcataacac aactcgagag
     6001 tggattggtt taaaaaactc tcgataaatg cgtccacttt attgacacag atccatggat
     6061 cgatcgcacc ttgaaattta aacccagaaa tagtcccatc atcttctata ccgattacca
     6121 gcttacctcc atcggcattt gcaaacgcta ctatgtgttt tataatatct ctaggcttaa
     6181 tccgtgcact ttttctatca aaatactgat cttctttttc acttataaac ttttgaatct
     6241 cataatccat ttaaaatttc ccttcttaaa tttttttatt atagagccgt atattcaatt
     6301 tccacaaaaa aataatctaa aacaaaacag acaccttcat tttgatatgt tctcccacat
     6361 atgaatcggt cgctatacag cattacatat agtaattatt catactgcga aataaatagc
     6421 tccaaatctt tagctggcgt agttaagctt aaattcgaaa tgaaagtttc attgactatt
     6481 ccactttcat caaatccctt cttcaaccaa tcagagttcc acaacgtaaa tcctttagga
     6541 ttagaaactt tcttacataa ttttgcacta taggatttct ctcttgaatg aattaaagaa
     6601 agtctttgta accaaatttc aactatatcc gtattaggag tattaacaaa ttttcttaag
     6661 attttatcca agtataagtt gatttcttct acagcaataa actcaaaaag cttacctaaa
     6721 atcacaatac aagcctccaa agttctagga ttcttttgca ttatattaac aagaatacta
     6781 atcaattggt agatatcatc tggtcttttg tttaaagtag aaactctatg tgtatagact
     6841 tcacttagcg ctttacttaa actcccgcaa tttggatatt tgtctcctaa aagctttacc
     6901 tgtagcaaat gcttttgaag gctaagctta aacttccact tattattact tttctctgta
     6961 aaacttgcat gtttaagatc ccagtaaatt ttatcaggct taattgcatc taaaattata
     7021 ttatccgata agaatgtttt atttgaattt agcttcaaat ttagatccga taatacttca
     7081 gataacaatc tgataatcac ttcagcttga tcttttcgaa ccgaaaaaat tctgtaatca
     7141 tctctgtaac gaagtatttt gaactcttta atctctgttt tttctatctt gttcagtaac
     7201 attttatcag catacccaag tactatttca gctacaaaat ccatcaatac atttccttga
     7261 ggtataccgt tcgtttgtcc attttgtaaa tatcgtattt tagaatctat taagtttcca
     7321 acgccttgag tatcttttct atgtgtctta gcccattctt ttgaatgtac tgcccacggg
     7381 atagtatgtg tgtacattga gctataacaa tcggtaatat ctgaatgaat gcagtagtca
     7441 tactccaaag cataactaat ctgtgcttgt tcaaggttct cccaccaatt taaaatagtt
     7501 tccgcagtat cgctctttga agaaatagat tccacaggaa gtgatataca ttcaattcgc
     7561 ttgtcggctt ggaattcttc aaacttattt ttgatgtgat tccaattatt tttctgtgta
     7621 atcccgttta ctaagtctac atatactaat ggatgtaata tttgaagggg tctccaagca
     7681 taattaccat ctttattagc taatattcga tgattaacat caactgcctc agagagcgat
     7741 ctcttatgtc taactagttc agtaaatggt ttgtttttta ataaatccac tgctttctca
     7801 atgcctactg ataaatcaat atagttgggc aacgatgtac taaaaaaact tgatgttttt
     7861 aaaaagaatt gctttgcttc ttgatgattc atctctaaaa tactttttct agtcctagtc
     7921 aaattcaaaa tctcctagat caattaagtt gtaatgtaag aggataccct aaggttacac
     7981 accctattta ctattatacc agtattcatt cgtgatacat aagggtgtaa cattaagaac
     8041 tctatctaaa agactacaag tagtcacctt taattgtgag taattttctt tatccaatcc
     8101 ttgtgcaacc ttgtcgtcac cccttgtata tccccgtacc ccggatgttt cggaatatca
     8161 aaaatgtgct caaattcctg attttcatca gtctcccact catgattcaa cggttcatgc
     8221 cactctataa accttgacca taaaatgttc tttctataaa actcaatagg aatttcaaaa
     8281 cagtaaaaat tgtatttgct aaaatctgta aaattatctt tacgagcata ctttaaatgg
     8341 caggtatctc gccacaacca aattaaatct cgatccaatg gtaactgaat tcgtttcgta
     8401 tactcttctt caagccaatg aagtgcacca actgcattcg gattatcgaa aaagacagat
     8461 aattcagtat agcgtttctt tggataaata aatccttctg aatcaacaga ttgcaaaaaa
     8521 tctactggtt gaaccgtgta cactttcatt tattcgccac ctttttttga ctacttatag
     8581 tcatttacca atttgaaatc tgcatacttt ttcattcaat tgagcccagc attaattgta
     8641 cacgagtttt cttcactaac ggatagtatc aggatatact aatactatcc tgaatagcga
     8701 aatgaggtag ccaaaatgag aaaagtcgat gaatatatca aaaagaaatc ccaaaaaagt
     8761 aaagtatggc aaagaacatt tgagttagag tataagaaac tctcagatag cctcaaaaag
     8821 gacacttata atccaaatga acaaagtaaa gcagataacc aataaagatt accctcccat
     8881 atttaacatt gcttcacgaa tcagtaatca atgactacaa gtagtcattg aagcaaatct
     8941 tcataagata cctgtagaat atccctaata gctctcaatt gatccagctt aatatgttgc
     9001 cggccacctt caatcttaac caatgtttct cttgtcatag cgattttact gagctggagt
     9061 tctcgcacca gttctgtctg tcccattccc ttttctaatc ggattcgtct gatattgcta
     9121 ccgattacat tttctttatc tattaacttt tcttccttca aaaatagcac gccttttgga
     9181 ctaaaattag tcctttttct tattaatctt aaagatttgg tatgctacaa tgggactagt
     9241 attagtccat attcaatttt aaggaggaat ttttttgaag aaatttgcaa tcattctaac
     9301 ctgtgcttta ctcacgggag ctataagtgg gtgcgccact tctgacacaa attctacaag
     9361 tactgaaaca agtacaacaa cctcagaaaa gaattatcct atacattttt ttccagttcc
     9421 agatggattt gaagcttatt atcatgataa aaaactagat gttccattgt cagaaagtaa
     9481 ctctattgaa aaagttttgc caattgaaat tgaaaaaaat caagagaata taaaactcaa
     9541 ggccgagctt cccatactga acagcacaga aattgattgg cacgagaata ttaaagtaac
     9601 tattgaaaaa ggagcgttag cacaacttta tactgaaaaa gttgaacaat tttttgagtc
     9661 tggtgccaat aaagactttt caaaaataag cgattattcg gataactttt ttaatgagac
     9721 ccaagatgaa atcacgaact caacttttat ggctgaagat atgtacaata tgacgttaaa
     9781 aaaaataaaa gcttacgacc atccattaaa tctgacacta aataatggat tactctcttt
     9841 tgtattagca ggttacgttg actatgaata ttcaccagat tatttggaga cacctcttca
     9901 acacgagaac aatgatatta attttagatt tgtctacgag tcaaaaagta aaacatggtt
     9961 agttgatcaa ttaagcacta taaacacaag cttccatgaa ttacctaaac ctgaaaatga
    10021 agctgatatg gtcattaaaa tcttctaaaa aagaagcgtt gaaggagaaa tattcctttc
    10081 aacgctcttt tttatatgct tagaaacata aataaattgt atactttctt tttagcccct
    10141 attacttttc acgaaaaata tcttttttcg acactagcgt ttctctactt ataatattgt
    10201 acacagtttt tgtattattg aaaaattact cattatttag cattaatcca catcgtttta
    10261 tcaatcgacc ttcatactaa actactttaa agtcatcttc cttctcattt ttcattaaat
    10321 cagatacata agcccatgta cccgttcgtt gtaaataaag ttcttcttgt agcagctccc
    10381 actttctctt agaaatcata atgacgccat cgttcaatcc atttttagaa tccctagtaa
    10441 tttctatttc agaatttgaa tgaactactt cttgcaacac tttacctata ttttttttga
    10501 agtttgttgg agtaatagta tccaaaatga acacctctct tctctatacc tctattatga
    10561 agcgcccatc aaataaagca agcgttgagg aataattctt tcaacgcttg ctttattaag
    10621 ctttcatttt gtagtttgac taataccaat aatctgatct ctaaacaatc gaaaaacttc
    10681 ttgtggttga taataatctg tatctatttc aaatgtttct gagaacaatt cattgaattt
    10741 ttctactgaa atgatgattt taccattctc ctctcgactg ttttctatta tttctgcttc
    10801 taattcaaat aaatctagtg acgaactaaa atcataaatc ctgtcatatt gaacaaaaaa
    10861 atcaagaaaa agttgctcaa actcttcctt atttatcact agtgtttcaa gcggaacaac
    10921 tttattttca tcagccattc ttccaatttc ctcactttca ttttcaaaaa atcgtaaaat
    10981 tgatatagat tctttgtctc taatcactcg tgatattatc ttgacagtcc tacatctaat
    11041 tcaattaaag tataagccac tttcaaataa aagattacaa agtaaatggc aagccaacaa
    11101 aataaccatc tgcttcacgt attacgattt gataattctg gatttcgctc ggaagttcat
    11161 aataataatt aacagtttct gctttgccac tttcaatata ttctggatta ttcaatatca
    11221 tagcatactc ataaagttct tcaccatttt tatttggttc tataatattt tgtgttggtg
    11281 gaaaatcgaa ggcgattttc tactaacact ttttcttcgt ttcaactcaa aaagagactc
    11341 acttttggta aaatttagtc gaccaaaacg tcatcctacc aaaggagtct ctc
//
LOCUS       DACEJZ010000090        11237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEJZ010000090 DACEJZ010000000
VERSION     DACEJZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.093"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..1129
                     /gene="gmd"
                     /locus_tag="IW656_002309"
     CDS             83..1129
                     /gene="gmd"
                     /locus_tag="IW656_002309"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ0837999.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1209..2222
                     /locus_tag="IW656_002310"
     CDS             1209..2222
                     /locus_tag="IW656_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838000.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2259..3203
                     /locus_tag="IW656_002311"
     CDS             2259..3203
                     /locus_tag="IW656_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ0838001.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3271..4584
                     /locus_tag="IW656_002312"
     CDS             3271..4584
                     /locus_tag="IW656_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0838002.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4668..5570
                     /locus_tag="IW656_002313"
     CDS             4668..5570
                     /locus_tag="IW656_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ0838003.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5575..7227
                     /locus_tag="IW656_002314"
     CDS             5575..7227
                     /locus_tag="IW656_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0838004.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7251..8117
                     /locus_tag="IW656_002315"
     CDS             7251..8117
                     /locus_tag="IW656_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0838005.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8159..8350)
                     /locus_tag="IW656_002316"
     CDS             complement(8159..8350)
                     /locus_tag="IW656_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838006.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8380..8919
                     /locus_tag="IW656_002317"
     CDS             8380..8919
                     /locus_tag="IW656_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838007.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9047..9400
                     /locus_tag="IW656_002318"
     CDS             9047..9400
                     /locus_tag="IW656_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838008.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9867..10343
                     /locus_tag="IW656_002319"
                     /pseudo
     CDS             9867..10343
                     /locus_tag="IW656_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10343..10537
                     /locus_tag="IW656_002320"
     CDS             10343..10537
                     /locus_tag="IW656_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838009.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10543..10828
                     /locus_tag="IW656_002321"
                     /pseudo
     CDS             10543..10828
                     /locus_tag="IW656_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10842..11208
                     /locus_tag="IW656_002322"
                     /pseudo
     CDS             10842..11208
                     /locus_tag="IW656_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3153 a         2303 c         2854 g         2927 t
ORIGIN      
        1 ttatcgtgta ttcttgaaaa gtattagaaa ttacgagcca tttatatggt cttatcactt
       61 acataggaaa ggataatgta tcatgaaaaa agcgctgatt actggtatta ctggtcagga
      121 cggttcctat ctggcagatt tcctgctaga gaagggctac gaagttcacg gtatcactcg
      181 ccgtgcttct atttccaata ccgctcgcat tgaccatttg atgggcaaga tcacccttca
      241 tgacggtgac ctgtccgact catcttctct gattcgtatc atcagcattg ttcagcctga
      301 tgaaatctat aacctcgctg cacagagcca tgttcaggtt tcttttgatg ttcctgagta
      361 ctccggcgat gttgatgctt tgggcgttat gcgtattctg gaagcttgcc gtattctggg
      421 cctgacgaag aagactaaga tttatcaggc atctacttct gagctgtatg gcaaggttga
      481 agaagttcct cagcgtgaaa ccactccgtt tcatccttac agcccttatg ctgttgcaaa
      541 gcagtatggc ttctggatca ctaaggaata tcgtgaagct tatggcatgt tcgctgtcaa
      601 cggtattctg ttcaaccatg aatccgaacg tcgcggtgag aatttcgtca cccgtaagat
      661 caccctcgct gccggccgca ttgcagaagg cctgcaggac catctggaac tgggcaacat
      721 ggactccctg cgtgactggg gctatgcaaa ggactatgtt gagtgcatgt ggatgattct
      781 ccagcatgag actcccgagg acttcgtgat cgctaccggc gagcagcaca ctgttcgtga
      841 cttcaccgag aaggcattcg cagcaaatgg catgaccatt cgttgggaag gcactggcat
      901 tgatgaaaag ggctacgatg ttgaaaccgg caagatgctc gtttgtgtaa atccacagtg
      961 gttccgtccg actgacgttg acaacctgtg gggcgacccg actaaggcaa agaccgttct
     1021 cggttggaat ccgcagaaga cctcttacga ggaactggtt aacatcatgg caaagcatga
     1081 ccgtgaattg gcaaagcgcg aagctgcaat gaagaacgca gtcaagtaag ctgacattga
     1141 ataaacatat ctgtttctat ctttccagtc acggcttcgg acatatcgcg cggaatctgc
     1201 ccattattat ggcaacactc tgccgatgcg aagcgaagat tacactggtt tgcggcgcaa
     1261 atcatctgga ttttgcaaag cgcaatttgg agcaggaact gcccaaagag ctgatagacc
     1321 gcgtgcgcta tcgtccaata aacacagata tcggtctgat taaccgtgag ggcaccctac
     1381 tggtggacac gggtaagctg accgagatct gtgaatattt tttggcaaag gttccttctt
     1441 tggcaaagac agaagccgaa tggctcaagc gagaagctgt cagcgcagtg ctttgcgata
     1501 tgccaatctg ggcaatggat gcctgcgata tggcaggagt acctttcctg tatgtaggaa
     1561 acttcacttg gacagagctg tatcgcgaat ttttacccga acatatttgg aaagaatatg
     1621 ccaagcgtta cggtaaaatt cagcacacca tgctttatgc gctaaataac gaagaaatgt
     1681 tggaatttct ccaaaacagc gaaaaaacac cgacttccgt tgtggcgcgt ccgattcata
     1741 acgacgaagt gcaacgcatt aaatcacagc atgaaaaacc gcctgtattt gtcgcactcg
     1801 gtatgtccgc gcggttcaaa cagccaattg atgtcagtca cgcgccgtat gatttcatta
     1861 caacgctagg tgttccgctt gtgggagaaa acgtaaccgt tatttctaac agcaccccca
     1921 atacacagga ctatatagcg gcggcagatt acgttattac aaaggctgga tggggaactg
     1981 ttgcggagtg tattctcgca cagcgtccga tggcactgtt cgcccgcgat acagttttgg
     2041 aagatagaac aactatttgt aaattgcaaa aactgggtct ggcaatttcc gtcacaccgg
     2101 aaaacctcca ccgcgtagag cgggtgattg aggacctcaa acagctagac acctcaaatt
     2161 ttgatacatt ttatgatgct gcgagcgaaa tcgcagacaa attgatttct ctcatgcaat
     2221 gagagatgct ctaaatttag aaaggagcaa gcattattat gatggaaaaa gacgcaaaaa
     2281 tctacgtagc cggtcatcgt ggcatggctg gttccgcaat cgtgcgcgag ctgaaccgtc
     2341 aggactacaa caatattatc acccgcaccc ataaggagct tgacctgtgc cgtcaggacg
     2401 ctgttgaagc atttttcgca caggaaaagc cggattatgt atttctcgct gctgcaaagg
     2461 ttggcggtat tatcgcaaac cagaacgctc tggcagactt catgtatgaa aacatgattc
     2521 tggaaatgaa cgtcatcaac tccgcatgga agaatggctg taagaagtta cagttcctcg
     2581 gttcttcctg tatctatccg cgtatggcac cgcagccgat gccggaaagc tgtctgctga
     2641 caagtgagct ggaaaagacc aacgaggctt atgcactggc aaagatttct ggcctgaagt
     2701 actgtgagtt tctaaacaag cagtacggta ctgactatat ctctgttatg cctactaacc
     2761 tgtatggacc taacgacaac tatcatccta ctcacagtca tgtgctcccc gctttgattc
     2821 gtcgcttcca tgaggctaag gaagcaggtc tgcctactgt tacctgctgg ggtgatggtt
     2881 ctcctctgcg cgaattccta tatgttgacg atctggcaaa cctgtgtgtg ttcctgatga
     2941 ataactatag cggtgacgag actgttaatg caggtactgg caaggaactg tctatcaagg
     3001 aactgaccga gatggtagca aaggttattg gttacgaagg cgagattctg tgggatacca
     3061 gcaagccgaa cggtacacct cgtaagctgc tggatgtttc caaggcaacc aagctgggat
     3121 ggacatataa gactgagttg gaagatggca tccgtctgtc ctacgaggac ttcctgaata
     3181 acccgatgcg tgctgaacgc taatttaccg aggttgctgt gatttttgag gattgcagca
     3241 gcctctttct tataaaaagg agagatcatt atgaatattg ttttactgtc tggcggttct
     3301 ggtaagcgcc tttggccgct gtctaatgac atccgttcca agcagttcat taagattttc
     3361 aaaactgaag atggatatga atccatggtt cagcgtgtct accgccagat taagaaggta
     3421 gataacgacg caactgttac gattgcaacc tctaagacac aggtttctgc tatccataac
     3481 caacttggtg aggatgtggg tgtatctgta gagccttgtc gccgcgatac cttcccggca
     3541 atcgcattgg ctaccgctta tctggtagat gtgatgggca ttgatccaga agagtccgta
     3601 gttgtttgcc cggttgaccc ttacgtagaa gatgactatt ttgaggccct gaaatctctg
     3661 tccgatcagg ctgacaaagg cgaagctaat ctggtgctca tgggtattga accgacttat
     3721 ccttccgaga agtacggcta catcattcct gagagcgcag agcagactgc tttcgtaaat
     3781 accttcaagg aaaaaccttc tgaggctgtg gcaaaggaat atatcgcaca gggtgcactg
     3841 tggaacggtg gtgtgtttgt ctacaagctg aagtatgtca tggagcgtgc acatcagctg
     3901 attgactttg tagattatca ggatctgttc aacaaatacg atactctgac taagatttcc
     3961 ttcgactatg ccgttgttga gcatgaggac aagattcagg tacagcgttt tgctggtatg
     4021 tggaaggatc tggggacttg gaatactctg accgaaggta tggaggaatc cattatcggt
     4081 aaaggtgtta tgaatgagaa ttgcgatggc gtacacatcc tgaacgaact ggatgttcct
     4141 gtgttggcta tgggtctgca tgatgtggtc atctctgcat ctcgggaagg tattctggtt
     4201 tccgataagg aacagtccag ctatattaag cctttcgttg acaagttcga gcagcagatc
     4261 atgttcgcag agaagtcttg gggtagttat aaggttatgg atgtagaagc agagagcatg
     4321 accattaagg ttactctgaa tgctagtcac tccatgaatt atcacagcca caagaaccgt
     4381 gacgaggtgt gggtggttct ggccggtact ggtaagacca tcgtggatgg atatgagcag
     4441 gttgttaagg ctggcgatgt gatcaccatg agtgctggct gtcggcatac cattatggca
     4501 gatactgagc tgaagctgat tgaagtacag cttggcaagg atatcaatgt taaagataaa
     4561 cagaaatttg agttagagta ttaaaaaacg gaggagggct cgttatacag aaaccgtttt
     4621 tattgaagcc ttccgcaaag gattatctgt gggggggaac cgcctgaatg atgaatttgc
     4681 aaaaggaatt gagatgtctc ctcttgcgga aacttggaat gctctacgca cccggatgga
     4741 ccgagctttg ttgtgtctgg tcagagtatc aggggatgac cttgacggag gtgttgcaag
     4801 aacacccaga gtttctggga acgcatccgc agaccaaagg agaattgccg atcctgatca
     4861 aattcattga tgcgaaaaag gatttgtctg ttcaggtaca tcccacagat gaatatgcaa
     4921 ttgaaaaaga aaacgggcag cttggaaaga cagagatgtg gtatgtactg gatgccagct
     4981 taatctatgg gctgcagaaa ataacagaag aagctgtgat ccgtgaagcg attgctcagg
     5041 gaactgttga gcgttatctt cagaaggttc cggtcaaaaa gaatgatgtg ttctttattg
     5101 aggcgggaac gattcatgcg attggtgcgg gtgcactaat tgcagaaatt caggaaaatt
     5161 ccaatctgac ttatcgtctc tacgattatg accgtgttga caaaaatggt aaaaagagag
     5221 aacttcatgt agacaaagcg ctagaagtag cgaacttgaa agggttgtca gaaccgaaac
     5281 agccgcttcg tgttctaaaa tatcgtgctg gtgtagctgc ggaattgctt tgcagatgca
     5341 agcatttcga ggtctctcgt atgattgtca acactgagag aagacagacg gtacactatc
     5401 gtgcggattc agtcagcttc cgtgtgttgc tgtgtgtcaa tggctgtggt accgttcgat
     5461 ttgataatga atcgttggac atctataagg gggactgcct ttttgtgccg gccgattcta
     5521 ctgaaatcag cattcacggg tcgattcagt ttctggatgt gagaggataa taatatgaat
     5581 tataaagtag aatatgagcg ttggctgaat catgccaatg cagatattat agaagaactg
     5641 cgttcctatt ctgagaagga cattgaggac tctttctata aaaatttgtc tttcggtact
     5701 ggtggtctgc gtggtaccat cggtgcaggc actaatcgca tgaacgtaca caccgtaggt
     5761 aaggcaagcc aaggtctggc agactatctg aataagaact atgaagccgc ttccgtggtg
     5821 gttggctatg attcccgtat caaatctgat gtgtttgcaa agactgccgc agacatcttt
     5881 gctgcaaatg gtattcaggt gcacttgtgg ccagaactgc ttcctgttcc tacagtatct
     5941 tttgcgactc gttatctgaa ggcagatgcc ggtgttatga ttacggcatc tcataaccct
     6001 tctaaatata acggctacaa ggtatacggt gcagacggct gtcagattac taccgaggct
     6061 gctactgcaa ttctggcaga aatcgaaaag ctggacatct ttgctgatgt aaagatggat
     6121 ggcgatgctt ctaagattgc atccattcag tcggaagttc tgactgcttt catcgaagaa
     6181 gtgaaggggc agtccgtcct gtttggcgaa aaagtggaca agaatgttgc tatcgtgtat
     6241 tctcctctga atggtaccgg tctggtgcct gttacccgca ccctggcaga gatgggatac
     6301 agcaatgtga ccgttgtgga agaacagcgt ctgccggacg gtaatttccc gacttgtcct
     6361 tatccgaacc ctgaaattaa ggaagctatg tctctgggta tggagtatgc caagaagtac
     6421 aatgcggatc tgctgttggc cactgaccct gactgcgacc gtgttggtat cgcagttaag
     6481 agcgcacatg gtgactatgt tctgttgtct ggtaatgaga ctggtctgct acttctggac
     6541 tacatctgtt ctcagagaat taagcatgga aaaatgcctg ctgaccctgt gatggtcaag
     6601 accatcgtca ccatggattt gggtgaacgt atcgcatcca actatggtgt tcgtaccatc
     6661 gatgtgctga ccggctttaa gtttatcggt gagcagattg gtatgttgga aaaggctggt
     6721 aaggctgaca gctatatctt cggctttgag gaatcttacg gctatctgtc cggtagctat
     6781 gtccgtgaca aggacggtgt taacgctgct tatctaatct gtgaaatgtt ctcttactac
     6841 aagactcagg gtatcagcct gctggataag ctgaatgaga tttatacaaa gtatggctat
     6901 tgtttgaaca ccctgcattc cttcgagttt gacggtgcgg caggttttac caagatgcag
     6961 gaaattatgg ttgaattcca caagggtctc gatataattg gtggtaagaa ggttattgaa
     7021 actttagact acagtaaggg cctgaatggt ctgccgaagt ctgatgtttt gaagtttatg
     7081 ctggaagata actgctctgt tgtggttcgt ccttctggta ctgagccgaa gctgaagacc
     7141 tatatcagcg tcagcgcaga gaacaaggaa gccgccgaag ctatggaagc tattatggct
     7201 gctgaactgg cggaaatgtt cgagtaagaa aagaaaggag aaatcttgta atggaggaac
     7261 tgttttctaa cgctttgaaa tcggtgaagt caaatcgtgt gctgtacacg gcatcgttct
     7321 ttgcaagatc ctctcttttg tatttgcagg aaattggaca gctgactgct ctgaaggcac
     7381 atacatctag gcgtacagga ttgtcatctt acctttgttt cctggtagaa gttggttcag
     7441 gcacattgga gtataagggg cagaaatata cgcttcaagt aggagatatg gtgtttattg
     7501 actgtttgaa gccgtatgtc catgagtcat cagaagatga tttgtggact ttgcggtggt
     7561 gccattttaa tggcattggt atgacggaag tctacaaaaa ataccttgaa cgaggtggtc
     7621 aaccggtctt taaaacatca aaaacagata agtatgctga cttgctgagg agccttcata
     7681 tggtggcaga gggacaggag tatgtccggg atatgaaaat caatgttcta ctcagtgaac
     7741 tgctgatgca tttgatggaa gattcttgga atcctgacaa agtgccgaat ttctctacaa
     7801 aaaaaatgga attgcagaac ataaaacaat atctggatga ccattacacg gagaagatta
     7861 cgctggatca gttgagtgca gagtttttta tcaataaaca gtacttagct aagatttttc
     7921 gtgaaactta cggtacaact gttgtcggtt atctgaatca gaaacgaatc acacaggcca
     7981 agcacttgct acgctttaca aatctaagta tggaagagat tgcaacaaaa attggcataa
     8041 cagatgcaaa ctatttttcc agaacttttt tcaaggtgga aggtgttcgt cccagcgaat
     8101 tccgcaagat gtggtaataa aaagccggcg gtgttgagcc gccggtcagt acctgtcgtt
     8161 aacccgcatt caaagcttta atctcagctt tgatttcagc acgaacacga tagtaatctt
     8221 cagtttcgat ttcggtatcc agccgagttg ctgtctttcc gcagtcagga caacgctctg
     8281 gctgttcgtc ggcagggaag ctgtagtggc aggaatcaca ggtgtaaatg tgatcgtcac
     8341 gtataatcat cacctaccaa tccagtgctt agatttttca tgcaattact attatatcga
     8401 attggtgatt ccacaaaaat ccgcttcttg ataaatgtac gattcagagt gatatatagt
     8461 actgtactgc caaagggaat accacaacgg cagaaatgga tgattcacat ggaagaaatt
     8521 atgcagatga ttttagggaa cgcaaaattc cagctcggac aggtggtttc gacccctgct
     8581 gtattggaaa ctttcaaccc cctgcagacc gcaaacggca ttcccgaagc cttgagcaac
     8641 ctttggttta gacaggaaat tagtattgcg gagatcgagg acgcagttct ggatgccaag
     8701 aacggcaagg agctagcaaa cagcctcaac cgcatcaagc ttttccgcaa gttcaccctc
     8761 gaccgagagc tggacggcat ggtcagactg aagggcgcgg acgtctggaa gaacatcagc
     8821 tacctcaagg tctacaccgc cacgccgcct aagaaaccga cccccccaag gttccagtcg
     8881 aacggctgga gccttttctt ctctcataaa tcggcataat gtatacaaat acagctccaa
     8941 atgattgtgt agtatatgcc tgtggattga cttgatatag tcgagagact atggcaatat
     9001 acacacaacc aaaggggaaa atcccaaaaa caaggaggaa acgctaatgg caatgagcga
     9061 aaaggcaaag aaatatttga aggagatcaa gggtgcaaag accatcccgg agctgaagga
     9121 tgtggagatc gcaatcaagc gagacggtat ccttgcttgg gcagagttca ccaagttgaa
     9181 cgaggcggtg gaagagaaga aggtcgccct tcgcaagaaa aagcaggaga ccagccttca
     9241 ggagatcctt ttctgggcct acaaaaagga atcagacaag ctgctgaaga tgatggacga
     9301 gggagcagac aaggatgcca tccagatgca ggtccagcgt tacgacagca tcggccagat
     9361 cattgcagag gcagacctcg aagatgagta tgaggtttga gaatacagcc tgaaccacta
     9421 agaaccaaat accgaaaggg acttctgaaa cagacagagg tccctttctt ttgctcactt
     9481 tggcggtcaa ttatgaacca aaaaccagtc gaatgattgt gtacattatg gctttcaaac
     9541 gacttgataa taatcacttt tagagtgata tatgtactac aaaaaacaaa agactggaga
     9601 aaacaacaga tgagcgaata cagaatcatt tatatcagcg acgaggacgg cggcgaggtg
     9661 gaaggatgtg gctacacggc tacgatggtt atgatgcggt cagaaacttc ttggaatttg
     9721 cctgcggcat cttgaaaatc ctgcgggtgc gattcatcag aagagtggac tgaaatacaa
     9781 gttagcagat agagaagaga gtcaggtttc gacttggctc tgttctctgt ctactggcag
     9841 aaatacattg aagggaggtg acgactgtgg caaccagagg aagaaaatca accccgacag
     9901 ccatgaagaa gctggaggga aatccgggca atcgcccact caatgcaaaa gagtcaacgc
     9961 cggaaaagaa ggcaccgacc tgtccgaagt ggctggaacc agaggcaaag aaggaatgga
    10021 agcgtctggc aaagcagatg gaggccatcg gaatcttgac agatgtggat atggctgcct
    10081 ttgccggtta gtgccaagcc tacgcccgct ggaaggaagc cgaggaattc attacccagc
    10141 acggtatcat cgtgaaaacc ctatccggct attggcagca ggtaccacag gtgtttattg
    10201 cccagaccta tctgaaaatt atgaaccgat tttccgagca gttcggtctg acgccttcgt
    10261 ccagaagccg tatcattgcc ggtgatggcg gtggtgtcgg agcagaagat gcgatggagg
    10321 ctctgttggg aggtggtagc tgatggcaga aaccagacca aagggctatc ccaaattaaa
    10381 ggaatacaca ccaaaccgat tcatgctttc ggaatgtcac tacgacaaag ccagagcgga
    10441 tcgggcggtg aactttatcg gacagctccg acacaccaaa ggcaaatggg caggcaaccg
    10501 cttctggctc ttgccttggc aggaacagat catctagtgt aatctcgctt aactgtatta
    10561 acggacgtag cgactttatt aagagtgttc atggtaggag cattagttgc aacaatgatt
    10621 gatattaaat ttttatggta ccaacgattg gcgatgtgac attgaatatg caaaactata
    10681 tcgatatgat attatctaaa atcttactcg ctgggatcgt cgaggtgttt attggttgtt
    10741 aggaaagaaa aatatgaccc aactcgagcg atttttatcg tattacttgt gtgtggcttt
    10801 atctgcattg tgtgtgattt cgaaataagg gagagccaaa aatgagtaga aaattgatat
    10861 tgattagtca cggaaaattt tgtgaggaat tgaaaaaaag tacaaaaatg attatgggac
    10921 ctcaagagaa tatctacacg ttacctttat taccagaaga tggcactgaa acatacagac
    10981 aaaagctctt actgtcatag attcgttaaa tgattttgtg gtactatgcg atcttttagg
    11041 cggtacacct actaatatca tttctaaact gataatggaa ggacaacaga ttaaacttta
    11101 tgtcggatta atcttcctat ggtcattgaa tttatcaact cgcaagtgat tggttcagag
    11161 cccgagtttg ttcgtgcagg atgtgaaagc attgtatctg tcaactaatt aattagtgga
    11221 atagtagaag atgaaga
//
LOCUS       DACEJZ010000091        10980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEJZ010000091 DACEJZ010000000
VERSION     DACEJZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.094"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..1434)
                     /locus_tag="IW656_002323"
     CDS             complement(58..1434)
                     /locus_tag="IW656_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0838010.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1562..2680)
                     /locus_tag="IW656_002324"
     CDS             complement(1562..2680)
                     /locus_tag="IW656_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0838011.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2822..3256
                     /locus_tag="IW656_002325"
     CDS             2822..3256
                     /locus_tag="IW656_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0838012.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3334..3867)
                     /locus_tag="IW656_002326"
     CDS             complement(3334..3867)
                     /locus_tag="IW656_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0838013.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3961..5139)
                     /gene="mutY"
                     /locus_tag="IW656_002327"
     CDS             complement(3961..5139)
                     /gene="mutY"
                     /locus_tag="IW656_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0838014.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5132..5929)
                     /gene="recX"
                     /locus_tag="IW656_002328"
     CDS             complement(5132..5929)
                     /gene="recX"
                     /locus_tag="IW656_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0838015.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6036..7418
                     /gene="rlmD"
                     /locus_tag="IW656_002329"
     CDS             6036..7418
                     /gene="rlmD"
                     /locus_tag="IW656_002329"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0838016.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7592..8170)
                     /locus_tag="IW656_002330"
     CDS             complement(7592..8170)
                     /locus_tag="IW656_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0838017.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8492..8962
                     /locus_tag="IW656_002331"
     CDS             8492..8962
                     /locus_tag="IW656_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0838018.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(9070..10296)
                     /locus_tag="IW656_002332"
     CDS             complement(9070..10296)
                     /locus_tag="IW656_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0838019.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3278 a         2190 c         1844 g         3668 t
ORIGIN      
        1 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
       61 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
      121 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
      181 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
      241 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
      301 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
      361 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
      421 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
      481 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
      541 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
      601 aaagccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
      661 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
      721 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
      781 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
      841 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
      901 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
      961 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
     1021 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
     1081 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
     1141 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
     1201 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
     1261 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
     1321 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
     1381 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
     1441 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
     1501 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
     1561 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
     1621 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
     1681 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
     1741 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
     1801 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
     1861 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
     1921 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
     1981 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
     2041 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
     2101 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
     2161 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
     2221 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
     2281 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
     2341 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
     2401 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
     2461 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
     2521 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
     2581 aaataaagtt tatttctgat gaaatcatga tcaatgcagc tatgatcaat aattcaagcg
     2641 accaaaacat tagtttagac tgtctaagtt tttcaaacaa tagatttcct cctttctttc
     2701 ttactagtat atattatatt cgttaaaaat tcttttattc tttttttatt tttgaaaact
     2761 tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag gtggctagac
     2821 catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg ctgtacggat
     2881 caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga
     2941 tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt
     3001 acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga ttctttctga
     3061 atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa
     3121 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
     3181 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
     3241 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
     3301 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
     3361 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
     3421 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
     3481 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
     3541 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
     3601 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
     3661 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
     3721 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
     3781 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
     3841 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
     3901 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
     3961 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
     4021 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
     4081 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
     4141 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
     4201 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
     4261 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
     4321 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
     4381 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
     4441 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
     4501 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
     4561 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
     4621 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
     4681 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
     4741 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
     4801 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
     4861 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
     4921 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
     4981 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
     5041 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
     5101 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
     5161 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
     5221 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
     5281 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
     5341 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
     5401 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
     5461 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
     5521 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
     5581 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
     5641 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
     5701 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
     5761 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
     5821 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
     5881 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
     5941 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
     6001 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
     6061 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
     6121 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
     6181 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
     6241 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
     6301 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
     6361 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
     6421 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
     6481 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
     6541 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
     6601 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
     6661 tcggcgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
     6721 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
     6781 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
     6841 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
     6901 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
     6961 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
     7021 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
     7081 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
     7141 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
     7201 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
     7261 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
     7321 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
     7381 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
     7441 ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg ttttatgaat
     7501 actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa aagggttgtg
     7561 acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct atcacttgat
     7621 aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta tatccttttc
     7681 gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt gctttgtcgt
     7741 ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga ttccaagttg
     7801 gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa ttccaagtaa
     7861 tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc tgttcatcta
     7921 aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat gtatttttgg
     7981 tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt aagtttcctg
     8041 ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca tgcgcaaaac
     8101 ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat aacgtactaa
     8161 tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct ttattgtatt
     8221 aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga
     8281 tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag aatcaaatca
     8341 tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt aataacaaca
     8401 attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga gccttcatgg
     8461 taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata ttcgtttttg
     8521 ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta gcggaaatcc
     8581 cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct ggatatttgg
     8641 gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt tacttaatca
     8701 caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt tcatttttaa
     8761 ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag caatcgatat
     8821 tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc atcatcgcct
     8881 ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc tattgaatta
     8941 ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta acacatagaa
     9001 aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta ttaaccaaac
     9061 tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat aaccataacc
     9121 attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg gtgtatagta
     9181 ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact gatcatatgt
     9241 gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat caccgtttgt
     9301 cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat ttttcaaatt
     9361 aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt ggatcgaaag
     9421 aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc cttcgccttg
     9481 caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta catcaagcat
     9541 gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa tttttctata
     9601 attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta cttcccgctg
     9661 acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag aataggctaa
     9721 acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat ccaaagtaaa
     9781 gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt ccatgccatc
     9841 caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa ttgaatccat
     9901 ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg ttccataacc
     9961 tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt tttgtttcgg
    10021 attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc ctaatccacc
    10081 ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc ttactctctc
    10141 agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg tttggctcgc
    10201 ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa cgaatacccc
    10261 taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt tcacattttc
    10321 taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc ataagaaaac
    10381 tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa attcttaact
    10441 atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc aatcgtttta
    10501 tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc aaataatcgt
    10561 gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc taccgttcag
    10621 attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt aaacccatct
    10681 tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa ctagtgattg
    10741 cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac gattttcttt
    10801 taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac aaatggtctt
    10861 ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa gaaaatgttc
    10921 cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt agccaaaaaa
//
LOCUS       DACEJZ010000092        10772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEJZ010000092 DACEJZ010000000
VERSION     DACEJZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.095"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            81..1127
                     /locus_tag="IW656_002333"
     CDS             81..1127
                     /locus_tag="IW656_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0838020.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1476..2108
                     /locus_tag="IW656_002334"
     CDS             1476..2108
                     /locus_tag="IW656_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0838021.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2696..4135
                     /locus_tag="IW656_002335"
     CDS             2696..4135
                     /locus_tag="IW656_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0838022.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4430..4648
                     /locus_tag="IW656_002336"
                     /pseudo
     CDS             4430..4648
                     /locus_tag="IW656_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4897..5112
                     /locus_tag="IW656_002337"
     CDS             4897..5112
                     /locus_tag="IW656_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838023.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5329..5585)
                     /locus_tag="IW656_002338"
                     /pseudo
     CDS             complement(5329..5585)
                     /locus_tag="IW656_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5772..6683)
                     /locus_tag="IW656_002339"
     CDS             complement(5772..6683)
                     /locus_tag="IW656_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838024.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6702..7706)
                     /locus_tag="IW656_002340"
     CDS             complement(6702..7706)
                     /locus_tag="IW656_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0838025.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7703..9826)
                     /locus_tag="IW656_002341"
     CDS             complement(7703..9826)
                     /locus_tag="IW656_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0838026.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9831..10751)
                     /locus_tag="IW656_002342"
     CDS             complement(9831..10751)
                     /locus_tag="IW656_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0838027.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2035 c         2020 g         3424 t
ORIGIN      
        1 cgtcaagtga aagtagtacg atttcaagta ccgaatcttc aacaacttca agtagtacaa
       61 gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat agaatcttca aaaaaggaag
      121 cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc acagcaaata gaatcaacag
      181 aatctttcca atcacaacca atactatctg gtaatgaaga aattcatttt gacaaaaatg
      241 aatctgtaga aaattttatt atgaaaattg gtgagtctgc gagaaaaatt ggtcagaaat
      301 atgatttata tgcttctgtt atgattgcac aagcgatctt agaatctgcc agtggtcaaa
      361 gccagttagc tcaggcaccg aattacaatt tatttgggat aaaaggaact tataatggaa
      421 actttgtcat tatggtaaca aacgaagatt tgggtaatgg aacgctttat acgacccaat
      481 caaaatttag agtgtatgaa aactatgagg aaagctttga agattatgcc aaattattaa
      541 ctaagggaat tagtggaaat aaagattttt atgcaggagc attaaaagct aatagtaaaa
      601 catatcgaga agcaactaaa ttccttacag gacggtatgc aacagataca caatactatt
      661 tgaagctaaa tgaattaatt aaaacctacg atttaaccaa ctatgataaa gaagttaaag
      721 gtccacaaat gagtaagact ggataccaag tgcctattca taattattca ataaccagtt
      781 tgtttggaaa tcgtggaaat gaatttcacc gaggacttga tatggcagct acacagggtg
      841 agccgattca tgcagttaaa gctggtaagg ttgttaaagc agagtttcac tactcttggg
      901 gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc gttatatgct caccagcaac
      961 aatacctagt aaaaactgga gataatgttt tgcaaggaca aataattggc tatgtaggaa
     1021 gtactgggaa tagtactggc ccacaccttc atttggaaat ttgcaaagat agtagtcttt
     1081 ctcagagcat gttagttgat ccgaaaacct tattattcgg agactaaaaa aggtaatcaa
     1141 tctgacaaag taacaagaaa atggttggat tcttagacct ttgatcagga tattgagact
     1201 ggaattgata tctcgattac gatgggtttg acaaataaaa caagtccatt atcgaatatc
     1261 gagtgtatta ttgccgtctt tatgctcata ttttgagcag gtttcactag atgaaaacaa
     1321 tttatctatc ttgaggattt cgtgtacgta tcgttcagtc ttgtattgta aattagtcat
     1381 aaaatcagac taagaaaaat tggaaatatt tttttctgat ttattaaaag ttaattgaaa
     1441 ttatatggta cttctttctt gggagggtag gtgcagtgaa ggaaagatat ggtcacctta
     1501 ctgttgttga ggagattact cataatggat ataaaaaata tcgttgccta tgtgactgtg
     1561 ggaatgaaac gattgtatat cgatcaaact tactatcagg tagaaccaaa agttgtggat
     1621 gtggaaatta taaaaatagg cataaatata aaaatttgac aaataaaact tttggtaagt
     1681 tagttgcaat caagccaact aaactgcgaa gtgaaggaac tgttgtttgg gaatgtcaat
     1741 gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact tctaaaaggt gatgtaaaaa
     1801 gctgtggatg tttgaaatca aaaaagcatc acattgaaaa taaaaaattc ggattactta
     1861 ctgcaaagta tcctttggac aataactgtt catggaaaac aaaatgggtt tgtcattgtg
     1921 agtgtggcag tatgtgtgag ataagttata gtaacttatt ttttggacat actcaaagtt
     1981 gtggatgttt aaaaaggcaa gaaacaagga catgtgttga agggacagta atagagtgtc
     2041 taacgtcaaa attaccgaaa aataatacaa gtggtgttaa gggagtttat cgttctaaag
     2101 agaaatagat tgcgtatata actttaaaaa aaaagaggta ctatttagga tcgtatgatt
     2161 ctattaagag tgcagctcaa gctagaaaag atgctgaaaa taaattgttt caacctttga
     2221 ttgacaaata tcgaggtaat cattaattat tgtggataat aattaacagt gatatataaa
     2281 catctataat atattattca tactattgat atttagtata atcctatgga attgaatatc
     2341 atagcaatgt atttgaaaag caaattggaa aacaaatagg tgcaacacca taaatatgaa
     2401 aaaattggga aaaagtagaa tggaaatttg cttacgtctc acgagatagc tcacactatt
     2461 aatctgaaga acaatgaagt cgacttttag aaattgctag aagaaagtaa agcgaattat
     2521 cactagttac tatagagtaa gttcaaataa agcatctcca acttacatgg ttacatgagg
     2581 atatcaaatc gaaattactc aagcttccta tggctagagt cgcgagaagt tgttgggcag
     2641 tagacatagg ttatattgtt cttagctaca cgatcattag aaaggaaatc ctattatgta
     2701 tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg tggattttga ttgctatatc
     2761 tatgtttacc ttcatgtcca cattggatgg cagtattgtc aatattgcac tacctaccat
     2821 ctcagatgat atgggcatac caatgaatca agcggaatgg gtagtgtccg tttatctgat
     2881 gattgtctgt tcttgtttgt tgctgtttgg taaaattggc gatagccgtg ggaaaatcaa
     2941 agtatttcgg attggtacgc tgatctttgt cttgggatcg ctgttgtgtg ggttcaatca
     3001 ttcgctaggt attttattgt tcgcacgggt ggtacaagcc attggtgcta gtatgactat
     3061 ggctacaaat gccggaatca tcacggaagt ctttcccata catgaacgtg gtcgggcatt
     3121 gggagcaata ggtgcgtttg tttcattagg ctcgattgct ggaccaggtc ttggcggctt
     3181 gattttgtcc caattttctt gggcgtacat tttttggatc aatgtaccgg tgggaatcgt
     3241 aacgatgttg attgctgaaa aattcttacc aaaagatatc accatgagcg gccatacaat
     3301 cgatggaggg ggctttgctg ccattgcggc tacgattatc agcttttttg gagcaatctt
     3361 tttggggcaa gaaagtggct ttttccatcc agtccccctt gtactgttcg taatttcagt
     3421 gattgccttt gtcagctttt taaaagttga aaagaaagca gcctctccat tgatttcttt
     3481 cggtattttt aagaacaaga tttttaccat gagcttgatt acagccttgt tgattttttc
     3541 ttccaacttc tttgtcaatg tagtgattcc tttttatctg caaaatgctc gcgggttgag
     3601 tcctagctat gctgggctgt tgatgatggt ctttcctttg atgatggtcg tcggggcgcc
     3661 tttaagcggc tacctgaccg accgggttgg cccggagatt ttggtattag taggcttggg
     3721 cttattgtcg gtaacccagg tcatgtatat gtttttgtca gtggactctc ctttgtggct
     3781 atacattgtc gctactgcca tcatgggtgc cggcaatgcc ttgttccagt cccccaataa
     3841 tacgatggtt atgagctcgg tcactatcga aaacttggga ctggcaggct cgatgaattc
     3901 ctttgcccga aatttgggga tggtcagcgg gatttcgctt tctacgacgg tcttatatca
     3961 agcgatgtct gctaagatgg gccagcaagt aactaactat atcgcaaatc ggccggatgt
     4021 ctttttatat ggaatgaaga ttaccttttt aggcagcttt gttttgtgtt ttgttgcctt
     4081 tttcctgact atctggcgta tcaaaaaaca aggaggtatt cagaaaaatg tctgaaaaaa
     4141 ccactgttga agaatttttg gaggcctgcg tatgggtgag tgggatttcc atggatgatg
     4201 gaagatgaag tgaacttcta aaagccgaaa aactcaagta ttatcttcaa aatactagtg
     4261 ctcaatgaga tgcttgattt gatcacaaga agactgcgaa agccgctggc aaccgcaagc
     4321 taggtatccc accgatggac tacgttttag aggctactgg ttatgaatac ggagccaacg
     4381 ctctcatctg cataagaata catcacccag attatgtaaa gattttcgat acttctgtca
     4441 aaaaatcatt aagatagata aatgatttcc gtctagtcaa attggttcag aatgtagaca
     4501 cacagatggt aagaagtcac tcacaattcg agaatggact tgtcctattt gccgtataca
     4561 tcataatcga aacgtcaacg atagtatcaa tattttgacc aagggctatg aatacaagca
     4621 ttgccttaga aaacatcaag aaccttagga acaacgggga tagcttggtt aataagagat
     4681 accgctgttt aggcttgcaa gtatactctt ttatcaagaa ttccgtggct ttagtcatga
     4741 gaggcttaat actatgaaaa ttttctttag catttttgat ttaggttcct tttctaatta
     4801 taataagatg accactacag taacctactg aaatatataa acagccactg tagtaccatt
     4861 ttcaaagtag tctatgagag gagaatcttc attcttatga acgctggaat tgcggatatg
     4921 aatttaataa aaaaaaccct taatgatttt acttccaatt caatatcaaa aggtactggt
     4981 atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa gatcagtgga aaagctgaat
     5041 cttttagatg ctataaaaat aacggaattt gctatgaaaa atggaaaagc tgagattgaa
     5101 atatggagat aattatcgtc tagtgaatta cttgccactt agctgaattt aacgatcctt
     5161 ggtgcttgaa gggggagatt cttgggaata gagcatactt gcaagcctat gtctttacta
     5221 acaacgatat ctcttattaa ccaagctatc cccgtagttc taaggctttt acttttatct
     5281 aagcaataat cgtattcaaa ggaaattttt gacccttaga aagtctcatt ttatcagacg
     5341 tgagcttagg cattgctgaa ttttgttaat tcggttttag gttgtgcttt gagcataata
     5401 tgaaagtatt ctttatcacg atttcattca tctaaggtaa tgtgatgtga ttctgaaatt
     5461 ctttcaaagg cagtttttgc ataagctgac attacatcat caatgacatg tttacaatgt
     5521 ttcactacta aaataagggg gtaaccaaga agaaaaactg aatgattatt tgtatctaat
     5581 tccatggtat atcaaccatt tcctatatag actgattatg acatggatta aaataaaaag
     5641 gcactatgtc ttaattgtcg gttttcgagt taccgaatgg cattcatcac ccacctatag
     5701 aggatgggcg acttctgcct gaaataagtt acactactta taacttctaa taaaattaga
     5761 ttgagtcatt attatttaac atcaacgatt ttccagtttc catttttttg taatactaat
     5821 ttgtactgaa agtagctagt ggtttttgat acattatcaa ggtattttat ctttgtactc
     5881 actagaaaat tttttccgtt ttgtagataa actggattct ctaactccga aaactttaag
     5941 tcaatattga ttggtcgcat cgcaccattt tcaacataat actccaattc tttttcagaa
     6001 gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg tgatttcttc cgttgttttg
     6061 gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat attttgcctt gtctggcttt
     6121 ccaatcattg ttggcaaact agttactaaa tgattcctgt tttcatcttc aaagattgtc
     6181 actcgatatg cagaagaaat tgattgcgtc ttttttccct ctgtaatatg ttgctccact
     6241 aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat ctagaatttt tacagattga
     6301 acttcggaac tcgtgggaat atctgctcga accatatctt ggattaaagc gagaccgttt
     6361 tctgtaaggt actgttcgag attactcatt ctcttttcaa ttgcttcttt gttatttttc
     6421 catgaaaaat attctttcgc aaaatcctct gtaaaacttt caacgccaga agtattcact
     6481 accttctctt ttataaccac tttttcatga gttgtatgtt gatcaatggc ggtgaaattt
     6541 ttataaacgc caaaagcaag actacaagac aataaaagcc aaagggcaag caccattttt
     6601 cgatgcttac ccacggatac aactcgttct tttttctttt ttgggctctt ttccttttga
     6661 ttccgttcaa tttttatctt cattcttttt cctcttttct cttatttgta tcggccagct
     6721 ccgataagat gctgccgcca atagttttcc gttagattcg tccaccccac tggatctcct
     6781 gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat gggtcacata agtaccggca
     6841 tcataggttg aatgaaagaa aactaaatca cctggtttgg catcttttag atcaatatga
     6901 gtcatggcat catattgatc ctgagccacc cgaggaagtt tcaggtcaac tttggcataa
     6961 caccattggg tcaagccact acaatcaaac cccgtttctg gacttgagcc accaaacaca
     7021 taggccgttc cttcatactt aaatgcctca tcaaaaattt cttggactat tttgtcatca
     7081 aactgagttg tagttaggta ttgcttcact aataaacagt aaaacatatt gccataatta
     7141 taccgccacc caccattttc cttaatcgcg attgggttgg ggtaatcgac tttttgacca
     7201 cctgattttt cctttgaaaa agattcagct aaagcaaaac tatgcttctt cccgtttttc
     7261 ccgacatgat ctagataccc gccaccatag ttataggcct gaataatact atcttgatca
     7321 caccctagtt ctttggaacg cttaaccaaa gacgcaaaat agagacagcc ttgtttgatg
     7381 gatgcctcgg tacttaatga attaggtggt aatccggctg actcagaact ttgcataacg
     7441 tcttccattg tgccaccaga ttccacttga ataatcgcca aaagaattgg aatatactct
     7501 gttatatccg attctttggc atacttttcc accattgcac ggtgtttcaa tacttcttct
     7561 gagacactag ttccgccagg agaagtgcca gaactaccat ttccctcact gtcttcacca
     7621 aacaacaaac ccacacacat caaaataccc ataaaagaga tggtcagaaa agttaatgaa
     7681 ataacaccca ctttcttcaa gttcattttt tctcacctga ccttgatttt ggacgatggt
     7741 tcattctttt ttgtactttt tgattggtct gctttggcag agtggacctc tgtttcactt
     7801 gttggaaaga atttttctta cttgctgatt ggcttttccc ttttgaaatg atttcttgac
     7861 tagttagttt tcgtttgatt tcgggattca gatttgctct cacaatccgc tttggtgctt
     7921 cttttttcag catcatatct ttctgaggtt gagctgcttt atctattgga atattcgccc
     7981 ttctttttcc ctgacgattt gttgcttcac tcagtatctt gcgtttttcg tccattttcg
     8041 catttcttcg ttgtcgttgt ttttctcgca aatccactcg atccgctttt tcttggaaca
     8101 gtccgcgctt gaattcttct ggtgcttttt tcgacttttc gattccttta tggagattgt
     8161 atttcaaatt cgttggcgta ttccttacct gatcctttac ttgtttagtc ttatctttca
     8221 ccaaagcttg ggtatccaaa actttgccga tttttctccc agccaaattc attcgggatt
     8281 gtttcttatt atttgaaact ggattctcct tagttaagtc agatagtatt tcgtgatcgt
     8341 tcggtaatcg tgaattcttt cgtagagaag aaccggatgg cttaaatttg gattttgatt
     8401 gttcctttgc caacttattt ccgactgctg caccaccacc aaaagccaaa gctcgtccca
     8461 catttcgatt gagttgccgc aattttcgat tcattaacat ttgcggtttt cgcatgacgc
     8521 gtcttcccat tgactggcta tcattactct ggaggttgaa catactcata atgtcgccca
     8581 atttgaaata gataccggca aaggtcacaa tttgcagaaa agcaaccatg aaaaatggat
     8641 aggttgccga aatattgaaa aacatcgtgg agatgctaaa tgcagtcgta atcaccaaag
     8701 taacacccgc tcgcatcata atcgtattga ataaacgaat aatcgccttc tttcctaaac
     8761 tttcataagt cggcaacatg gacaataaga aactaattgg cagaaacatc gcaaagataa
     8821 tgaagagaat ttgcgagaac aacataatcc ctgttagaag aaaaacaaag aaggaaatgc
     8881 caatattgaa caataccaaa aagaccacgg tccctaaacg gttcatggtc ttggtaatac
     8941 tgagattcgc attgtcattg tcttcaatct ctgccttgac cgcttcttcc cgatctttgc
     9001 ctttgttttc atctggactc acagatagaa tcttgttgac ccggtcttca ccaatttctt
     9061 ctatgttgga atcatcaaat tgcagtagta accaaggttg ttggacttga atcgaaaaca
     9121 aactatcccg aatcaaatcc acactatctt ttccttggct ttctgaattg ggtacaacga
     9181 tctcggttcc aagggtaagg gccgcttcac taacatctga gctaaagtca ttgatttttg
     9241 tgatataggt aggcgcataa gcaatgaacg aacccgatag tagaaaaatc atcacaaaat
     9301 taatcacggc tcgaactgct ttcgtggttt ctcgttttaa taatccggta tacgccacat
     9361 acacccccag agctaaaatc atcaataata gaaacccaaa ataaaatccg gaggtctgga
     9421 gtccattttc agtaatacca gccaatgttt ggatattctt tccaatactt tcagccgtat
     9481 ccgaaatgaa atctaatttg taagcctctt gaaccacata acccgtggcg ttcgacaaat
     9541 acaaactcac cgtccaaatg aaattagtaa tggcataaag accatacatc acgcttttcc
     9601 ctaagccatc accccaattc caaggcaacc aatcccaaga gctatccaca aagaaatcta
     9661 gttgatagtt gttcagtgaa tatttggaat atagatttcc tgcttcaact gtatcatcaa
     9721 ctagccctgt cgcctcggct accgtaccaa ctaaactcaa tagaactatc agagtgagta
     9781 tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc tcactccatt
     9841 ctctcttgaa caggtggacg ggtatcaaag gcatggaaga gttcttcaaa gactggatgt
     9901 acctgaacca cgcctacccg accatacaaa tcctgcatca agcattggcc attttccaaa
     9961 tctcgtaggc gtttttgatt gctttcatct tctcgatcca agccaaagaa ttccaaggtt
    10021 ttcttgattt catgaatatc ggtactgcga aacgcaaact tcaacccgat attattcttc
    10081 aagcgttcat ccagtaaatc atcactattt tgcgtgacaa agtaaacccc ggcattcata
    10141 gcccgcccag cacgaacgag cttattcgat aatgttttcc cttgcgctac ttgaagaaaa
    10201 ctccacgcct catctaagtc tacaattttg aagatttctc gattactatg gataaagtcc
    10261 aaggcaaaag tggaaatgac gattagcatg gaaacactaa gcaattccat ggtggtatat
    10321 tcttcaaaag tggtttctgc atctggtaaa accaaatccg ctacttgaat gatatttaac
    10381 tgcctatcta aactaatcga ctgggtaatc gaaccatctg agaaaagcaa atgggcaaaa
    10441 tcataatcgg taaaggactc gatgtgatcc gcgatatttt ccgcaactgg tgagccttct
    10501 tttcgaagct cattaattac ttgtaataat ccccgttgat tgctttgggt aaccgtccga
    10561 atggccttac gcaacactgg aaacttctcc ccatctcgac tagaaattcc tgttaaaaag
    10621 gtcagaatat caattgctag actttcagaa tccttctttt tctccataat cacataagga
    10681 tcaagtaatc cttgattttt tggcttacta gttaagttga gaatattgat ttcatccgca
    10741 atttctggca aggtttcttt ccagccaccc cg
//
LOCUS       DACEJZ010000093        10754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEJZ010000093 DACEJZ010000000
VERSION     DACEJZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.096"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IW656_002343"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IW656_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0838028.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IW656_002344"
     CDS             1995..4661
                     /locus_tag="IW656_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0838029.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IW656_002345"
     CDS             5025..5777
                     /locus_tag="IW656_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0838030.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IW656_002346"
     CDS             complement(5799..6953)
                     /locus_tag="IW656_002346"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0838031.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IW656_002347"
     CDS             7138..9579
                     /locus_tag="IW656_002347"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0838032.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IW656_002348"
     CDS             complement(9909..10613)
                     /locus_tag="IW656_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838033.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3432 a         1931 c         2266 g         3125 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaa
//
LOCUS       DACEJZ010000094        10713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEJZ010000094 DACEJZ010000000
VERSION     DACEJZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.097"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1235)
                     /locus_tag="IW656_002349"
     CDS             complement(<1..1235)
                     /locus_tag="IW656_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0838034.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="IW656_002350"
     CDS             complement(1228..2820)
                     /locus_tag="IW656_002350"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0838035.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3330..4055)
                     /locus_tag="IW656_002351"
     CDS             complement(3330..4055)
                     /locus_tag="IW656_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838036.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4209..4535)
                     /locus_tag="IW656_002352"
     CDS             complement(4209..4535)
                     /locus_tag="IW656_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838037.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4572..4937)
                     /locus_tag="IW656_002353"
     CDS             complement(4572..4937)
                     /locus_tag="IW656_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0838038.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4974..5189)
                     /locus_tag="IW656_002354"
     CDS             complement(4974..5189)
                     /locus_tag="IW656_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838039.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5318..5653
                     /locus_tag="IW656_002355"
     CDS             5318..5653
                     /locus_tag="IW656_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838040.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5729..7726)
                     /locus_tag="IW656_002356"
     CDS             complement(5729..7726)
                     /locus_tag="IW656_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838041.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7824..8099)
                     /locus_tag="IW656_002357"
     CDS             complement(7824..8099)
                     /locus_tag="IW656_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0838042.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8331..9065
                     /locus_tag="IW656_002358"
     CDS             8331..9065
                     /locus_tag="IW656_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838043.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9104..10246
                     /locus_tag="IW656_002359"
     CDS             9104..10246
                     /locus_tag="IW656_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0838044.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3450 a         2003 c         1582 g         3678 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
     3001 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
     3061 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
     3121 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
     3181 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
     3241 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
     3301 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
     3361 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
     3421 aaccgaagtg gaatatcttt accaactttc tctgtcactg cattactgat acctgttaga
     3481 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     3541 gtgattcctt tatcttcatt tacaagtttc caagtaggtg gtatagctgt gtatgtttct
     3601 acaaatgaat taagtttttc attaagtgac ttctctttta tatttccatc taaaaactct
     3661 gtatatatgg cttgagctat actaaaacaa agctggcctt gttctaataa ttttacatca
     3721 ttagttagta aagctgtgtt cacgttatca atcgcttcaa gaagagtatc tttggtgtcc
     3781 ctaacagctt taggtgtgcc ttctggaatt tccctacttg atacctcgtc cattttatct
     3841 tccataaacc ccttaaaatc atatctttct cccaaatcca tagaagtttt tgagaaagct
     3901 ttcatttctg agtctatttc ttcaataata tttaacatgt ctgatacata ttgtttaggc
     3961 tgattcgttt ttaatctata tgtggtaaat atctcaattc ctccaattat caataaaatt
     4021 gccaaacaaa tccataataa ttttttcttt ttcatttttc ctcctataca ctgctgttat
     4081 aacagtgact aatgtaaaga ttttcccagc aatcatcaga ctattattta cagggaatca
     4141 aaaaaactcc ttagtaagct tacaccttta ggagttcctg tttgaacggt atgcataata
     4201 catttaaatc aatttaacat taacacctca gtatatggta tctctacata tacacggtct
     4261 ttttttggat agtactcgac atttatattg atagcacttc cattgtctaa cgtagcttta
     4321 gcccggtacg tgttatcacc attatcatga gtgatcgtca catcatcaca tgtccgattt
     4381 aagttatact gttcttgtaa aatctgagtt accaatggta cagatgactt ctctagtcca
     4441 gatgaacccg tcattcttga aaaaattata cctataatag ccaatacaac gatacctata
     4501 atgacaaact tcttattcat atttacttta ttcaactttt tatctcctat ctccaaaatg
     4561 attttctctc atcagtaact cattgacatc cccaatagta caccaaagat ggctccagct
     4621 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tctctcgata taagtaaccc
     4681 ataataacag caacagcacc aaataccaca ggaataaatg ctaatgatac taccatagaa
     4741 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     4801 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     4861 gtttcttgta atgctccgca aaactggcaa tacttacttt catttgttat ttctttacca
     4921 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagctaactg
     4981 tttactttat taaatacatc agaaaacagc tcatttattg gtacacttaa tatctcagaa
     5041 agcattttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     5101 tgctgtgtaa cattcatttt tttggctaac tcagtttggg aaagtccttt ttcttttctg
     5161 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     5221 aatatgatgt atttcaactc ataaaatata taaatacttc aaatgtcatt acaaatacaa
     5281 caacttgttg tactctactg agaagggggg tgtattaatg aaacagaagc aatatgcaaa
     5341 ggtcattaga gaaaagcgaa aagcactcgg gtggacacaa aaagaattag ctaaaaaaat
     5401 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagaac ctaacctcga
     5461 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     5521 tgacatagat tatgaggaag agtttttggc tctctatcgc tctcttagta ctgaagatgc
     5581 aattcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     5641 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     5701 ataagattaa gagctatgga taacctgtct agtttaatat aattgggtct cttctattcc
     5761 ttactctccg tccatcatct cttgttacac ttgtaaattg ataaatagga actaattcaa
     5821 ctcctgataa tatcaattct tttattactt catctatccc tgcccctttc gctagatata
     5881 ctaaaaaact actgcttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     5941 caagagtgaa tccttgttta acaaagtagc ctctgttttc tattaaagca tttttcacgc
     6001 tgttatccct gacatttcgt aggcgttcac aaacagtgtt aataagttct ttttctgtca
     6061 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     6121 attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     6181 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaaatt
     6241 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     6301 cgtctaatcg atagacattg cttctcttat gtttaaagtt tcctaacgct ttctcttcct
     6361 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     6421 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     6481 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     6541 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     6601 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     6661 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     6721 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     6781 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     6841 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     6901 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     6961 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     7021 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     7081 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     7141 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     7201 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     7261 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     7321 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     7381 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     7441 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     7501 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     7561 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     7621 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     7681 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcct
     7741 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     7801 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     7861 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     7921 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     7981 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     8041 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     8101 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
     8161 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
     8221 catcgacata cacataatac ctcgataaaa aaggggtgtc aatagttttg taaaatttat
     8281 ttacaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
     8341 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
     8401 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
     8461 ttgacaaaaa gactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
     8521 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
     8581 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
     8641 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
     8701 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
     8761 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
     8821 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
     8881 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
     8941 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
     9001 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
     9061 tctagtggta agtactcctt gtaaactaag aggagatgat ttcatggcaa aaatatcaaa
     9121 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
     9181 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
     9241 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
     9301 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
     9361 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
     9421 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
     9481 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
     9541 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
     9601 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
     9661 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
     9721 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
     9781 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
     9841 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
     9901 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
     9961 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
    10021 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
    10081 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
    10141 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
    10201 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
    10261 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat atatcaaggg
    10321 ttctagctcc tataacaggt ttacccaaca ctgccttcca atgactcttt tgggaatcag
    10381 aggaaagaat caggctttat agagatattt catctaacaa attactttag actgacagta
    10441 tatatcagca ggtactgtca tcgggaacgt agccaatatc gtatccaaaa cagcaggtag
    10501 agaagctatt tgaggattca agttattcta tttcaaccat gaagttaagt tcattaaaaa
    10561 taaagatagt tggtatttca ataatttttg tatagagtta atcactttat tgatgctaat
    10621 tcatcttgat tggtattata atcgtattca agttgcgtgg cgtgcttttg ctagaaaata
    10681 tataactgtt ctttataaag aaatataagg att
//
LOCUS       DACEJZ010000095        10706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEJZ010000095 DACEJZ010000000
VERSION     DACEJZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.098"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..144)
                     /locus_tag="IW656_002360"
     CDS             complement(25..144)
                     /locus_tag="IW656_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0838045.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IW656_002361"
     CDS             complement(159..1343)
                     /locus_tag="IW656_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0838046.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IW656_002362"
     CDS             complement(1599..1853)
                     /locus_tag="IW656_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838047.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IW656_002363"
     CDS             complement(1878..3020)
                     /locus_tag="IW656_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0838048.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IW656_002364"
     CDS             complement(3080..4432)
                     /locus_tag="IW656_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0838049.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IW656_002365"
     CDS             complement(4503..4847)
                     /locus_tag="IW656_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0838050.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IW656_002366"
     CDS             complement(4857..5231)
                     /locus_tag="IW656_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0838051.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IW656_002367"
     CDS             complement(5244..5558)
                     /locus_tag="IW656_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0838052.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="IW656_002368"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="IW656_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0838053.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..9640)
                     /locus_tag="IW656_002369"
     CDS             complement(8978..9640)
                     /locus_tag="IW656_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838054.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10257..10487
                     /locus_tag="IW656_002370"
     CDS             10257..10487
                     /locus_tag="IW656_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838055.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT         2953 a         2361 c         1817 g         3575 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccagaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcatctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgattttt ccaaatgata cgtccatcat tttgcttcac gataaaaact cctaatagtt
     9661 atttagatag aagcctatca catcaacttt ttagtagaaa agtacatgtt ttgtcttttt
     9721 tgctaatagc tgaccctttg agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt
     9781 ttggtatttt tcgtattata tttgtcgtca agctgactac taaattttgc cctattttga
     9841 cgacactgat agtcaatgtc ggacaatcta ggacaatttt taagggaata tgattgaaaa
     9901 atggtccaaa acagccaaaa atggcttaaa actgtgatag agaaaaaaga aaaagccctt
     9961 gatatacaag gacttttcac aaatttcaga tttccaagga agttttccgt gtttatcttc
    10021 tgatttcttt gatacgagca gcttttccgt gtaatgcacg taaacaaaac cactatattc
    10081 tattatgtat ctaataccct caaaacagct ataaatcaac aataccgaca tataataaac
    10141 actatgttca atctgagata tatcaaaggt aaacgtattt ttgccccctt tttgcccctc
    10201 aaatatcgct ttacaacgag aacgtaagtt cgtataatcg ttttgaggtg atttttatga
    10261 tggaagactt tattagaaaa aatatcagtg atgaatatgc agacttttat gagcaaagca
    10321 gcaaaaaaga taaattccag atggatgttt caatcttagc tatattagct ttttccgaaa
    10381 ataaccaacc tgtaattgcg aaaaaagaaa cagtattatc tgaaggcaaa ataaaaactc
    10441 gatatatatt agaggtagaa actaagttta aaaatagatc ggagtaatgg ctatgctttt
    10501 taatgagacg caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    10561 taaagtatgg gattcagaag ttttcttttt agcaatcgaa gatagtttat taatcaatct
    10621 ctactattct aataagaact atttcaaaat tcctgctgcg aaaactagaa tgaagaagga
    10681 tgtatatttt ttgtttgata tcgtga
//
LOCUS       DACEJZ010000096        10468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEJZ010000096 DACEJZ010000000
VERSION     DACEJZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.099"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..524)
                     /locus_tag="IW656_002371"
     CDS             complement(<1..524)
                     /locus_tag="IW656_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0838056.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVK"
     gene            complement(691..2811)
                     /locus_tag="IW656_002372"
     CDS             complement(691..2811)
                     /locus_tag="IW656_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0838057.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3041..4219
                     /locus_tag="IW656_002373"
     CDS             3041..4219
                     /locus_tag="IW656_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0838058.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            4235..4993
                     /gene="aroD"
                     /locus_tag="IW656_002374"
     CDS             4235..4993
                     /gene="aroD"
                     /locus_tag="IW656_002374"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0838059.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5016..5501
                     /gene="ybaK"
                     /locus_tag="IW656_002375"
     CDS             5016..5501
                     /gene="ybaK"
                     /locus_tag="IW656_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0838060.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5572..6825)
                     /locus_tag="IW656_002376"
     CDS             complement(5572..6825)
                     /locus_tag="IW656_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0838061.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6942..8291
                     /gene="gdhA"
                     /locus_tag="IW656_002377"
     CDS             6942..8291
                     /gene="gdhA"
                     /locus_tag="IW656_002377"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0838062.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            8403..9749
                     /locus_tag="IW656_002378"
     CDS             8403..9749
                     /locus_tag="IW656_002378"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0838063.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10057..10443)
                     /locus_tag="IW656_002379"
     CDS             complement(10057..10443)
                     /locus_tag="IW656_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0838064.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3324 a         1903 c         2145 g         3096 t
ORIGIN      
        1 tttttcaccg atttgaattc atcgaaacac accacttctg gtaacggctg gttcaaagaa
       61 acggttttag gccgaaggct gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc
      121 atacgtgcaa tcaaagacat agaaataggc tcacttaata gctcaagaat ggcttgtttg
      181 actctgcggg caatggagca gcgacgatcg ctaactgaat cttctgctaa aaaagtcttt
      241 ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca caatggtctt gtagttactg
      301 acatcattca ataagatgga tacttttttc cagccccatt taatgatcgt ttgcttattt
      361 tgatagtggc aatggaagca ttcctctggt tgataggtca atgtgccatc aaaaacaaaa
      421 catgtttcgt cattaatgcg ctctttgcga aaggcatctt catgaaaggt aagatttagg
      481 tctagaatat ctagtaactc tttggtaaac tctgaatagg acatcttgaa acactccttt
      541 gaatggtttt cgtcgacttt attctaaagg atttcaaggt gtctgtttgc gtttattaga
      601 aaaaagaaaa agtattggca acagaaggcg tcaccaacac tagatattat agatcctaat
      661 tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg tcgttccgtg
      721 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      781 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      841 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      901 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      961 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
     1021 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
     1081 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
     1141 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
     1201 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
     1261 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
     1321 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
     1381 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
     1441 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
     1501 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
     1561 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
     1621 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1681 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1741 atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1801 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1861 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1921 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1981 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     2041 gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata
     2101 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     2161 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     2221 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     2281 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     2341 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     2401 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     2461 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     2521 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     2581 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     2641 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2701 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2761 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2821 cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag ctcacttatt
     2881 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2941 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     3001 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     3061 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     3121 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     3181 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     3241 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     3301 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacgggat
     3361 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     3421 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcat
     3481 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     3541 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     3601 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3661 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3721 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3781 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3841 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3901 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3961 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     4021 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     4081 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     4141 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     4201 aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg gattgaatat
     4261 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     4321 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     4381 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     4441 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     4501 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     4561 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     4621 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4681 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4741 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4801 aaatgctttt ttctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4861 tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4921 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4981 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     5041 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     5101 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     5161 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     5221 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     5281 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     5341 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     5401 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     5461 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     5521 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     5581 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     5641 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5701 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5761 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5821 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5881 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5941 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     6001 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     6061 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     6121 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     6181 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     6241 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     6301 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     6361 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     6421 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     6481 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     6541 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     6601 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6661 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6721 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6781 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6841 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa
     6901 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     6961 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     7021 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     7081 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     7141 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     7201 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     7261 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     7321 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     7381 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     7441 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     7501 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     7561 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     7621 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     7681 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     7741 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     7801 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     7861 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     7921 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     7981 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     8041 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     8101 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     8161 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     8221 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     8281 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     8341 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     8401 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     8461 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     8521 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     8581 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     8641 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     8701 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     8761 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     8821 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     8881 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     8941 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     9001 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     9061 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     9121 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     9181 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     9241 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     9301 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     9361 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     9421 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     9481 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     9541 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     9601 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
     9661 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
     9721 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
     9781 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
     9841 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
     9901 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
     9961 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
    10021 ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt
    10081 tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct
    10141 tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag agtcaccaga
    10201 agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt
    10261 gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg
    10321 cattggagct ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc
    10381 ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt
    10441 catgaaagat ctcctttttt atttttat
//
LOCUS       DACEJZ010000097        10432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEJZ010000097 DACEJZ010000000
VERSION     DACEJZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.100"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IW656_002380"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IW656_002380"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0838065.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IW656_002381"
     CDS             1007..1927
                     /locus_tag="IW656_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0838066.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IW656_002382"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IW656_002382"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0838067.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IW656_002383"
     CDS             3233..4711
                     /locus_tag="IW656_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0838068.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IW656_002384"
     CDS             4988..7048
                     /locus_tag="IW656_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838069.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IW656_002385"
     CDS             7131..7700
                     /locus_tag="IW656_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0838070.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IW656_002386"
     CDS             complement(7875..8681)
                     /locus_tag="IW656_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838071.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IW656_002387"
     CDS             8819..10333
                     /locus_tag="IW656_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0838072.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3702 a         1739 c         2055 g         2936 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACEJZ010000098         9854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEJZ010000098 DACEJZ010000000
VERSION     DACEJZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.101"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(183..1031)
                     /locus_tag="IW656_002388"
     CDS             complement(183..1031)
                     /locus_tag="IW656_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0838073.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1195..2097
                     /gene="gnd"
                     /locus_tag="IW656_002389"
     CDS             1195..2097
                     /gene="gnd"
                     /locus_tag="IW656_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0838074.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="IW656_002390"
     CDS             2099..3100
                     /locus_tag="IW656_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0838075.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="IW656_002391"
     CDS             3097..3381
                     /locus_tag="IW656_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0838076.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="IW656_002392"
     CDS             3414..4211
                     /locus_tag="IW656_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0838077.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="IW656_002393"
     CDS             4225..5058
                     /locus_tag="IW656_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0838078.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="IW656_002394"
     CDS             5076..5933
                     /locus_tag="IW656_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0838079.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="IW656_002395"
     CDS             5955..6464
                     /locus_tag="IW656_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0838080.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="IW656_002396"
     CDS             6501..6902
                     /locus_tag="IW656_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838081.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="IW656_002397"
     CDS             7014..7367
                     /locus_tag="IW656_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838082.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="IW656_002398"
     CDS             7401..7844
                     /locus_tag="IW656_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0838083.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="IW656_002399"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="IW656_002399"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0838084.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="IW656_002400"
     CDS             8939..9652
                     /locus_tag="IW656_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0838085.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3275 a         1563 c         2180 g         2836 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tcca
//
LOCUS       DACEJZ010000099         9846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEJZ010000099 DACEJZ010000000
VERSION     DACEJZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.102"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..243)
                     /locus_tag="IW656_002401"
     CDS             complement(<1..243)
                     /locus_tag="IW656_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838086.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(358..936)
                     /locus_tag="IW656_002402"
     CDS             complement(358..936)
                     /locus_tag="IW656_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0838087.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1013..1543)
                     /locus_tag="IW656_002403"
     CDS             complement(1013..1543)
                     /locus_tag="IW656_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0838088.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="IW656_002404"
     CDS             complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="IW656_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0838089.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2400..2795)
                     /locus_tag="IW656_002405"
     CDS             complement(2400..2795)
                     /locus_tag="IW656_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0838090.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2792..4204)
                     /locus_tag="IW656_002406"
     CDS             complement(2792..4204)
                     /locus_tag="IW656_002406"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0838091.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="IW656_002407"
     CDS             complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="IW656_002407"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0838092.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5059..6519)
                     /locus_tag="IW656_002408"
     CDS             complement(5059..6519)
                     /locus_tag="IW656_002408"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0838093.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6689..7846)
                     /locus_tag="IW656_002409"
     CDS             complement(6689..7846)
                     /locus_tag="IW656_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0838094.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7880..9250)
                     /gene="radA"
                     /locus_tag="IW656_002410"
     CDS             complement(7880..9250)
                     /gene="radA"
                     /locus_tag="IW656_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0838095.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9313..9840)
                     /locus_tag="IW656_002411"
     CDS             complement(9313..9840)
                     /locus_tag="IW656_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0838096.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2505 a         2207 c         1780 g         3354 t
ORIGIN      
        1 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
       61 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
      121 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
      181 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
      241 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttacca
      301 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
      361 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
      421 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
      481 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
      541 tgcttctttt aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa
      601 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
      661 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt
      721 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
      781 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
      841 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
      901 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
      961 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
     1021 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
     1081 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
     1141 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
     1201 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
     1261 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
     1321 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
     1381 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
     1441 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
     1501 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
     1561 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
     1621 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
     1681 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
     1741 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
     1801 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
     1861 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
     1921 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
     1981 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
     2041 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
     2101 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
     2161 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
     2221 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
     2281 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
     2341 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
     2401 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
     2461 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
     2521 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
     2581 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
     2641 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
     2701 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
     2761 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
     2821 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
     2881 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
     2941 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
     3001 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
     3061 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
     3121 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
     3181 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
     3241 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
     3301 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
     3361 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
     3421 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
     3481 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
     3541 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
     3601 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
     3661 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
     3721 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
     3781 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
     3841 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg
     3901 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
     3961 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
     4021 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
     4081 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
     4141 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
     4201 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
     4261 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
     4321 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
     4381 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
     4441 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
     4501 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
     4561 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
     4621 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
     4681 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
     4741 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
     4801 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
     4861 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
     4921 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
     4981 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
     5041 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
     5101 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
     5161 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
     5221 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
     5281 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
     5341 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
     5401 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
     5461 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
     5521 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
     5581 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
     5641 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
     5701 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
     5761 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
     5821 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
     5881 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
     5941 cataccatcg cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
     6001 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
     6061 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
     6121 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
     6181 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
     6241 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
     6301 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
     6361 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
     6421 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
     6481 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
     6541 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
     6601 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
     6661 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
     6721 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
     6781 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
     6841 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
     6901 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
     6961 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
     7021 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
     7081 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
     7141 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
     7201 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
     7261 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
     7321 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
     7381 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
     7441 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
     7501 tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
     7561 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
     7621 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
     7681 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
     7741 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
     7801 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
     7861 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
     7921 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
     7981 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
     8041 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
     8101 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
     8161 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
     8221 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
     8281 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
     8341 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
     8401 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
     8461 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
     8521 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
     8581 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
     8641 cgattttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
     8701 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
     8761 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
     8821 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
     8881 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
     8941 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
     9001 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
     9061 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
     9121 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
     9181 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
     9241 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
     9301 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
     9361 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
     9421 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
     9481 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
     9541 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
     9601 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
     9661 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
     9721 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
     9781 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
     9841 cggttt
//
LOCUS       DACEJZ010000100         9793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEJZ010000100 DACEJZ010000000
VERSION     DACEJZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.103"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            142..3420
                     /locus_tag="IW656_002412"
     CDS             142..3420
                     /locus_tag="IW656_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0838097.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3961..5868)
                     /locus_tag="IW656_002413"
     CDS             complement(3961..5868)
                     /locus_tag="IW656_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0838098.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5998..6873
                     /locus_tag="IW656_002414"
     CDS             5998..6873
                     /locus_tag="IW656_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0838099.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6953..7927
                     /locus_tag="IW656_002415"
     CDS             6953..7927
                     /locus_tag="IW656_002415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0838100.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8116..9252
                     /locus_tag="IW656_002416"
     CDS             8116..9252
                     /locus_tag="IW656_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0838101.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9389..9733
                     /locus_tag="IW656_002417"
     CDS             9389..9733
                     /locus_tag="IW656_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0838102.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3192 a         1805 c         2058 g         2738 t
ORIGIN      
        1 ctctttatta tcaacaactt aagttatatt ttttcttttt ataaaataca cttattataa
       61 ttttgttttt tattgacagt aaaaataaat taggtaatac tttaagtaaa cgctttctaa
      121 tttgttacgg gaggaataca cttgttaaaa aaacgtataa aatacagtgc gctaatgatg
      181 aatctcctta tgctcagtac cccggtttta atttctgtcg ttgaagttgc tgctaacgaa
      241 caacagtctg tgaacgaaga aaatgcacac gtgaacattg aagggatgtc aacaatggag
      301 gaaataccaa gaactagtga agatatggat acggaagaag gaaatgatcc tatgatgaaa
      361 gaatcagaaa atgaaaagac aaaaaagcaa tctgaagaag caaatgaaga gtggaaaggg
      421 gttgttttcg gagaaagcac aagtgctagt aggtatgcca ttgaaccatt agaagacggc
      481 gtgcggctct cttccacgaa taacggcgga aaattccagt caagcggatc agatggaatg
      541 acctattatt atcaagcaat ccccaaagac atcaacttta ggatgcgggc gacgatcgaa
      601 atagaatctt ggacctatac gaatgcccaa gaaggatttg ctttaatggt aagagacgca
      661 gtaccaaaaa atcatttgtt tgggcaggca ttttattcaa attcatttgc catactaggc
      721 agtcggatcg aatatgtctg ggactcggat agacaagaag tggtcaacac gagcggcagc
      781 aaatatacga tgcgacttgg attggggaca cgggcaatca caggaatatc atctgaagat
      841 ccgcaagctg cgccagctcc tgggagtgtc tcagtggaaa caacaccttt agagacttca
      901 gcagggttct tggaaaaaga aacaggttct tataatatat tcggaaacgg tcatgggaat
      961 ctgtttccgg ccacgaactc gattactaaa ctggacgtag agatgaaaaa aacgaatact
     1021 ggtttagagg catattatta cgatcctgaa acaggagaag aaatggcttc tgacatcatg
     1081 tatgactggg aaaaattata cgcttcagat gaatcggtta tttatgcagg atttgctgca
     1141 gcaaggaata tgacgatcaa ggtagaagga atcgaagttg cttactcaga tcctgcgaca
     1201 gatcctcctg ggcaagaaag acctactcgc ttgattgatc cgatttacac tgtgacctct
     1261 agtaatcatt caggaaacca agatcatact atgtccttta gagcaaatgc tgacggatta
     1321 ttgacaatta aaaataaagc aacaggagag gtgcttaaga acgcaaaaaa cttggctatc
     1381 actgcgaatc gggaatttga ttatcagaca aaactgatgg aaggaacgaa cgaatttact
     1441 ttcagtttta cgccagataa aaactatccg ccagaagaat acgtagagat gactagttat
     1501 gaaacaaaag agattcttca tacagtggac taccgtaaac ctaaatattc gacagtttat
     1561 gtaacaccag aagggtcaga tgcaggaaac ggaagcagac aaaatccatt atctctgaca
     1621 caagcattga gatacgcata tgctgggcag acaatcgtca tggcgcctgg aacttattcc
     1681 ttcgaaagag gattgacgat tccaaaaggc gttaacggga caagagaaaa cccgattcag
     1741 ttgattgcag agaaaaatcc agaaaacaag cgtcctgtac ttgattttaa aggaactgga
     1801 aatgggatga cactgttcag tcactactgg ggtttacgag ggtttgacat tacaaatagt
     1861 gcagctatgc aaaaaggatt acagatttct ggaaatcata atttgatcga agagattcat
     1921 gcttatcgga atggaaatac aggaatccaa atcagcggta gctcaaatga tcctttcgaa
     1981 gcatggcccg cgcataactt agtcaagaac tgtacatctt ttgaaaatgc cgatccagga
     2041 tttgaagatg ccgatggatt tgcggccaaa ctgacaatag gagaaggaaa tgtatttgac
     2101 ggttgtatcg cgtatcataa tgcagatgac ggttgggacc tttttgctaa aaatgaaaca
     2161 ggttcgatcg gaaaagtaat catcaaaaac tcagttgctt atcgaaacgg ctgggtacca
     2221 ggaattgagg gagaaggtaa cggtaatgga ttcaaaatgg gcggttcttc tttaacgggc
     2281 cctcatgaat tgatcaatag tttgtctttc gaaaatcttg cgaaaggaat cgattccaat
     2341 agcggaacag atattatcgt caatagcagt acaagtttta ataacggttc atataatgtg
     2401 gctctttata cctcttcagc aggaaacaca gactaccatg cttctggtat cttatctttc
     2461 cgaacagaga atcttgagat gagagagcag atccgcccat tgaatcaaaa cgaagatcaa
     2521 atctaccact cgttgaacta ctattggaat gaacgagaga aacaatcaga aaatactctt
     2581 ggacatacca tatcaaaaga ttggatcgag tctctaaata catgttcgaa agaaggtcag
     2641 cagcctgtta cgcgctatga aaatggaagt atccatgttc atggactgat gcagattaaa
     2701 ccggggaatc gtcaaacaga aaaggaaaga gtggatggtc ttccattatc tgtaggtgct
     2761 atttttgacg gagaaacctc accgtcttcg gaatatccag agtatgcaat cgtacaagaa
     2821 ccaaatcaga caaaaccaaa tgaacaaaat aatccgatta ttatacaaga agcaaatggt
     2881 gtggcggaga atgcagaagg aaatatcatt gaactgaagc cttttacagt ttttgtgtca
     2941 actttcacac caaatcaagg aacgcctgtc tttgaagata tgcgttattt cccagaaaag
     3001 atccaaatcg ttgaaacaca gtcaagtgct caattttcta atcataccgc tattttttca
     3061 gcaccttcaa aaggagaata tacgctagaa gtcacttatc gttcggaaat atttgatggg
     3121 gaaaaatggt ctgctcatac agaatctgtt acccagcaaa aagcgatcac tgtgcgagaa
     3181 actgccgatg tgcccccggg aaatgacaaa ccagaaagag aaaatccagg aaatgaatca
     3241 ccaaatggaa aaccaaatgg aaaaccttct catccggata ggacagcaaa agaaagtgaa
     3301 aaaaatggca ctcaaggact cccaaaaacg ggagaattta ataacccgtt gctagcatta
     3361 gctggaggaa ttttgttaat cggtgtagtt gtttatgtca tgaaacagcg aaaaaaataa
     3421 gtggaatttc tccttttatg atgtaaaaca attgactgag gaaagtaaaa atagaaggaa
     3481 atgagaccaa tagatgtctc acttccttct atctttttat agatgaggtt ctccttgctg
     3541 cttttgattg gctgttctta ttacttgatg caggacagct ttttcacaat ctctccacta
     3601 aacggtgttc aatcagctga ccctcggtat tagctcaaca aacggcaaaa tatgaggaac
     3661 tatttttccg tttttttctc taatactcag gtcaggacgc tgatttcaca acctctcgat
     3721 aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa
     3781 gccattttca caaaattttt cttactactt gaaactaagc agctttttca caacctctaa
     3841 tacatggtat tccagaagta acttcttcga aaaataagcc gaaatgtcac aaaaatttga
     3901 gaagcgattt tcccaaattc cttcttattt cttgaagctg accacttctg gcacaacctc
     3961 ttaccagatt cgcactcttt tttctggcgc tagatacata gcgtcttgtt ccgtgatacc
     4021 aaatgtttca tagaattcgt caaggttttt tggttgcacg tttgctcgga gtttagcagg
     4081 ggcatggaca tcgatttgca gtaaaagttg ttggtattct ttttttgctt ttgtacgcca
     4141 aatcgttgcc caattgacaa agaattcctc gatagaaaga tctgattccg tttttgctgc
     4201 ttccaatgca cagcttagac cgcctgcatc agcaatattt tccgaaacgg ttaattttcc
     4261 gtttactttt ccgtcagcaa aaggcagacc atcgaattct tcgatcattg cttgtgcttt
     4321 gttttgaaat tgtgtaagat cttcttctgt ccaccagttg ttcaagttgc catattcgtc
     4381 aaataaagcg ccgttattat caaatgcatg ggaaatctca tgagcgataa cagctccgat
     4441 tccaccataa ttagcagagc ttgattgttc tagactgtaa aatggtgctt gaaggatcgc
     4501 tgccggaaag acgatcacat tccgaaatgg atgataataa gcattgaccg tgtcagcact
     4561 catttcccat tctgaacgat ccactggttt gttccatttg ctgaaacgat cttttaatgt
     4621 gagtttgctg aaaacaagag tatttgaaag caaagtacca ccctgatctg caggtactgt
     4681 tttgaactgg tcgaataatg ctgggatctt atcggggtag cctacttgga tacctaagtt
     4741 atttaatttt atgacggctt tttcttttgt tgcatcccca agccagtcat tttcttctag
     4801 acgtttttta taaacggcga tcattttttt caccatttgt tctacgtctt ttttagcagc
     4861 ttcgccaaaa tatttttttc cgtagtaatc gccaactact tgatcgaatt gtccagaggc
     4921 aaggtaatac gctgcttttt tctgaggcat tgcttcgtct gttccagaca atacgcgaga
     4981 atagattccg ctgacttggc ggaattcttc tgatagataa ccactaagag aacgtactgt
     5041 tttaatgatc atccagcttt tgaataacgg aaaatgagct tctgtcagga tgtcattcaa
     5101 atgatcgaag tacactggat cagttacgat cactttatct ggagttgtcc caatcaaacc
     5161 cgtgacaagt tttttcaagt ccagtttatc tgtgtattta ataaatgtat caaaagattg
     5221 cggattatac attttgctgt aatctgcgtt ttcttctgca ctttttacat gaggagcaat
     5281 caatttatca aattgcactg cttgttccgt gattgcttct gcctgacttt tttctttgcc
     5341 ggcaagctct aataactgaa cagccatatc aaagaaaacg cctattagtt gtgcaccgtt
     5401 gggatggtct gatgcataat acgtcttatc tggaaggaat aaagaaggcg gataagcaaa
     5461 gaaggcgttc gtttgcgcat tcttcatatc tgcatctaca tccagattaa atggcaatgg
     5521 taatgtatcc aacagccatt ctggaaattg cttattcaat tcgtcgaaag actgcaatga
     5581 ttcaactttt tcgattatag gttgaagcgg ttgagcatgc aactcatttc ttcggtcata
     5641 atcgtttgcc agttgataat atgcaaggaa atgtttcatt ttttctgaag acactttttc
     5701 tggatgggca agcagatcgt ctgtttctcc catcaaaagt tggtcgatcc cgtcaactaa
     5761 atcttggaaa ccgcctgtag ctggtttatc tgcagggatt tttgctgttt tttgccattc
     5821 ttcattgact gcttcataga aatcttgttt caatcgttct ttgttcattc atcgatcgct
     5881 ccttttttgt ttcgtgtgcc cattatctta caaaatgaag gaattaacaa atagaacata
     5941 aaaaattgaa aattcgaaca atcaaactga tttgtttttt tcaatccttc acttataatg
     6001 aaaaaaagga ggcgatccca actgaatcaa gtagtggata aacctttata tttcaaagta
     6061 gataaaaaaa ttaaaaaatt agccagcact cagcagctgc agtcaagaaa aagtaaattg
     6121 ctttttttag ctctggtttt tgaagatcaa agttatgtga ttatcgatca atcaggacat
     6181 ccggtagaat attctccagc aaaatatact tatcaagagg gtctttcttg taaaaaatgg
     6241 aagctgatca atgaagagcc aatcgagtta tctagatgga tcaacagaaa agaagatatt
     6301 cccgttttga tcgaagaaaa aaggtcagga aaggaactcg caaattgctg ggtgggattg
     6361 ccagaagaac gttttttgag gtataagaaa tgggctaccc cctcaggcta tctttgtggt
     6421 acttacgcgg cagcagttct ccttgcctat tatcaagact atcgtaaggg gtggatgctt
     6481 ccaaatgaga ttcgcaagaa aaatacggct gattcgttgg ttttgacgaa ggcattgcgt
     6541 agccagatcc aaccactagg acttcctacg attccttttc aggttagtac agggatttct
     6601 agttttttaa agaagaatgg caatcatgag cgtgcacgag caactcttct aggttcatgg
     6661 cagcgagcta caaaacgtat acgtgaaggc aaacctgtga tgattggtat tttgaaagta
     6721 cttggcagta catatggcaa tcattgggtg acagcgtacg cttattttga aactgaaaca
     6781 ggagaacgtt attacaaagt acacgacaac tggggcgatt atcataaagt cattcctgcc
     6841 agttggagta acggaacagt ttcacttcct taagaatttg ctgaaattaa cagaaaatct
     6901 tggaaaaata tgttaaacta tcgaagaata gagaagtttt ggggaggact acatgagtaa
     6961 taagtatcag gatgacacgt tgaagatttt tagtttgaat ggcaaccgtc cattagctga
     7021 aaaaatcgcg aaagtgttcg gaacagaatt aggtaaaagc gtcgtgaagc aatttagtga
     7081 tggcgagatt tccatcaata tcgaagaaag tattcgaggg gatcatgtgt atatcgttca
     7141 atcaacgaat gcacctgtca atgattacta tatggaacta ctgatcatga tcgatgcaat
     7201 gaagcgagca agtgcgaaaa cgatcaatgt cgtattgcct tactatgggt atgctaggca
     7261 agaccgcacg gcgaaacctc atgaaccgat cactgcgaaa ttgatcgcta acttgatcga
     7321 agaagcaggg gcaactcgag tactgacttt agatctgcac acggttcaag tacaaggatt
     7381 ctttgatatt cctgtagata atttatttac gatgccgttg tttgctcatt attaccgtca
     7441 attaggtctg acgggagatg acatcgtagt tgtttcgcca aaaaacagcg gtgttcagcg
     7501 tgcacgtagt ctttcagaat atttgaacag cacattagcc atcgtagatc atgcagatga
     7561 tgcttcaact gagggtggtt atgtgatcgg tgatgttcaa ggaaagacat gtatcatggt
     7621 agatgatatc ttgaacacag gagcaacgct tgcacgagca gcaaatgtat taaaagaaaa
     7681 cggcgcaaaa gaagtatatg cctgtgcgtc acatggatta ttgtccgatc atgcgaaaag
     7741 tattttagac aatgcaccaa tcaaagatat ttgtatcact gattctgttt tcacagaaga
     7801 aaatcgtcat ccagaaaact taacaatcgt tacatgttca gaattaatgg gtgaagcggt
     7861 caaacgcatc catgaaaata caccgatgag tccgttattc cgtctggaag aaaaagaatt
     7921 cgaataaaca aaaaaaagag ggaaagaagt tgtgacagaa gtgagcagct tcaaggagct
     7981 gcgacaagac ttttgtcaca gctccttttt tctatgccca tgaaacgtcc tctagacaaa
     8041 aatgagctct tctttggttt gttcgctatt ttatgctaga atgtgcctag caataatttg
     8101 gaagggatga atgaagtgaa aactatttat ctggatcatg cggcaactac ccccatgcat
     8161 ccacaagtaa ttgaagcgat gacggcaatc atgcaagaca cgtttggaaa tccttcaagc
     8221 atacatggat ttggtcgcca cgcgcatgaa aaattggaaa cagcaagaca aatcattgca
     8281 gacagtctgc aagcaaaacc tcacgagatc atctttaaca gtgggggaac agaaggggat
     8341 aatacagcta tcttagaaac agcgttctcg cgtcaaaaag aaggcagaca tctaatcact
     8401 acagctatcg aacatcctgc tgttcttaat accatgaaat atttagagac aaaggggttt
     8461 gacgttacat atctgcctgt agatgaaaaa gggaatctgt cgatcgaaga ggtcaaaaaa
     8521 gcgttacgag aagatacgat cttggtctct gtgatgtacg ggaacaatga aatcggaaat
     8581 ttgatgccaa tcaaagaaat cggtgaactt ttagcggatc atccagctta tttccataca
     8641 gatgcggtac aagcgtacgg aaaccaaacg atccatccag aagaattagg aatcgattta
     8701 ttaagtatct ctgctcataa aatcaatggg ccaaaaggcg tcggtttctt atataaacga
     8761 gacggagtaa atatccctgc attgctttta ggcggggagc aggaagaaaa gcggcgtgca
     8821 ggtacagaaa atttagcagg gatctggggt atggcaaaag cagttgaact gctgtcacct
     8881 gaagaacgtg aagcacgtaa caacaaatat aaaggattcc agcaattgat tcttgataca
     8941 ttagaagcaa acgaaatccc ttatcaagta aatggtgatt taacgaataa actccctcat
     9001 gtgttgaatc ttcgcttaat tggcgaagca aatgatttgc tactgatgaa gcttgatctt
     9061 aatggggtcg ctatttctac tggttcagct tgtacagcag gaaatatcga gccttcacac
     9121 gtattagaag caatgtatgg agaagataca ccgatactaa aagaatccat ccgtatcagc
     9181 tttggtttgg gaaataccga agacgacatc cagtatttcc ttgatcaatt agttcgtgct
     9241 gtcaaaaaat aactagtgaa gtcattctta tatgaacaga tgtctgagag aacgccttca
     9301 ttttttaggg aacttaactg aaaaacctag taaaaatcag gataaacttt tataataaag
     9361 agaaactaat tttatcgagg tgaaaatcat ggcatttgaa acaacagcaa cggtactggg
     9421 cgcaccaatt agctatcgtt tgcatcctga tgcaaaaaga tatacattgc gcgataatgg
     9481 atttacagaa acaaacggag gcaattatca attgatccga ccattagatg ccacgccgca
     9541 aagcaaagaa ggattcaaat taaaaatcac tgtatcaaaa gatattcaaa cattgaaaat
     9601 gtcgatcact acagcaaatg gattgaaagc agtcaatatt ttcaaagatc caaaacagaa
     9661 aatgagccaa gataaatttt attttctgat ggatgggatg attagccgtg ggttattcga
     9721 aaaagtcgaa tgatctgtgg atataaaaag acaactagtt ttgaaacggc ctattgttag
     9781 atgactaact ttg
//
LOCUS       DACEJZ010000101         9317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEJZ010000101 DACEJZ010000000
VERSION     DACEJZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.104"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..2122
                     /locus_tag="IW656_002418"
     CDS             <1..2122
                     /locus_tag="IW656_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838103.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="IW656_002419"
     CDS             2119..2739
                     /locus_tag="IW656_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0838104.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3432..3812
                     /locus_tag="IW656_002420"
     CDS             3432..3812
                     /locus_tag="IW656_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0838105.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="IW656_002421"
     CDS             3794..4708
                     /locus_tag="IW656_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0838106.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="IW656_002422"
     CDS             4948..5637
                     /locus_tag="IW656_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838107.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="IW656_002423"
     CDS             complement(5758..5844)
                     /locus_tag="IW656_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0838108.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="IW656_002424"
     CDS             6489..7226
                     /locus_tag="IW656_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0838109.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="IW656_002425"
     CDS             7254..7793
                     /locus_tag="IW656_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0838110.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(8291..8752)
                     /locus_tag="IW656_002426"
     CDS             complement(8291..8752)
                     /locus_tag="IW656_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838111.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="IW656_002427"
     CDS             8885..9259
                     /locus_tag="IW656_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAQ0838112.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3597 a         1414 c         1454 g         2852 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt atcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt
     8101 aaaaaaatga cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag
     8161 ctttagaaag ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     8221 ttggtagagg ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaataactag ggggaaaaac attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccatg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DACEJZ010000102         9157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEJZ010000102 DACEJZ010000000
VERSION     DACEJZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.105"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            66..1253
                     /locus_tag="IW656_002428"
     CDS             66..1253
                     /locus_tag="IW656_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ0838113.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1234..2538
                     /locus_tag="IW656_002429"
     CDS             1234..2538
                     /locus_tag="IW656_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0838114.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2564..3577
                     /gene="citC"
                     /locus_tag="IW656_002430"
     CDS             2564..3577
                     /gene="citC"
                     /locus_tag="IW656_002430"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ0838115.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3581..3874
                     /gene="citD"
                     /locus_tag="IW656_002431"
     CDS             3581..3874
                     /gene="citD"
                     /locus_tag="IW656_002431"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ0838116.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3879..4784
                     /gene="citE"
                     /locus_tag="IW656_002432"
     CDS             3879..4784
                     /gene="citE"
                     /locus_tag="IW656_002432"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ0838117.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4777..6336
                     /gene="citF"
                     /locus_tag="IW656_002433"
     CDS             4777..6336
                     /gene="citF"
                     /locus_tag="IW656_002433"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ0838118.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6333..6890
                     /gene="citX"
                     /locus_tag="IW656_002434"
     CDS             6333..6890
                     /gene="citX"
                     /locus_tag="IW656_002434"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ0838119.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6883..7746
                     /gene="citG"
                     /locus_tag="IW656_002435"
     CDS             6883..7746
                     /gene="citG"
                     /locus_tag="IW656_002435"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ0838120.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7980..8389
                     /gene="tnpA"
                     /locus_tag="IW656_002436"
                     /pseudo
     CDS             7980..8389
                     /gene="tnpA"
                     /locus_tag="IW656_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9026..>9157)
                     /locus_tag="IW656_002437"
     CDS             complement(9026..>9157)
                     /locus_tag="IW656_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0838121.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3193 a         1420 c         1855 g         2689 t
ORIGIN      
        1 ttttttattt taaaaaaagt gttaaagtgg ttttgtaatt aaaaaatgta aaaaggagtg
       61 aaggtatgct tgatgaagtt ttagaaatcc ataaaaaaaa tgtaggtgtt ttacggatgg
      121 aaacaacaga agccgtgcaa gatgatcaag atttagcaaa aatgtacacc ccaggtgtag
      181 cagaactaag tttaatgatt gagaaaaatc atgctttggc aagagaatta acaattagtg
      241 gtaaattagt ggccgttatt acagacgggt cggcggtatt aggtcttgga aatgtaggaa
      301 accaagctgg attaccaatc gtcgaaggga aagcgctgct ttataaaaat ttagcaggtg
      361 tcaatgcgat tccattagcc atcgaacaaa aatcagtaga cgagattgta caaacaattg
      421 tcaatttgca aaatagtttt gcagggatcc atttagaaga tattgcagca ccgaagtgtt
      481 ttgagattga agaaaaatta caagaaaaac tttctattcc tgtgtatcat gacgatcaag
      541 aaggaacagc gatagttgtt ttagctggat tgatcaatgc tgctaagatc caaaggaaaa
      601 cattaactga acttcgagtt ttgatcaacg gcatgggagc atctggggtt gcaacagcta
      661 gactattaat cgctgctggt atcaaaaatc taacattggt ggataaacaa gggagattaa
      721 agaagcaaga tagtacatta aataaatatc aaagagagtt gttggatcat atacatgata
      781 caaataataa tgcaagcgtt ttggaagatg caatcgttgg acaagatgtt tttatcggtc
      841 tttcaatagg aaatgtttta acgcctgaaa tgattggaaa aatgaatgat aatccgatta
      901 tttttgcttt agccaatcca gtaccagaga tcacacccga tttagcaaaa gaatctggtg
      961 caaagctaat cgctacaggt tcttcgaaat atcctaacca agtaaacaat attttagtat
     1021 ttcccggatt gttcaaggga ctattactat cgaaagcgaa taaagtcgat ctttcattac
     1081 aacttgaagt agccaaacaa ttagctgcta aggtcgcaga gcctacggta gaaaaattca
     1141 tccctaatgt ttttgatgaa ggagtcgcaa acgtcgtttc acaggcagtt ttggattatt
     1201 tacaaaaact aaaagaaaac gaggataata tacatgctga aaacattaaa tagaattaaa
     1261 attggtttta ttcctttatc tctttatatc attttggtac tcgcgtatct tgcaatgacg
     1321 ataagcaata tcgttccaga aaatgatatg ttagtttctt tgggtattat gactgttttt
     1381 gcttatttat tagaagaaat tggtaaaact attcctattt taaaagattt aggtggtaaa
     1441 gtattagttg ttacattttt gcctgcattt ctagtctata aacattggct acctcaaagt
     1501 actgtaactg tggtttctga ttttatgaat aataacaatt tcttgatgtt ctttatcaca
     1561 ctactagttg tcggcagtat tgcttcaatg aatcggcaaa cattggttaa agcaacttca
     1621 cgtattatca ttattttaat tttatgcgat gtgattggtt ctttgatagg aacaggtgtg
     1681 ggaatactcc ttgggctatc tgcttggaat gcgtatttct ttatcgttgc gccgattatg
     1741 gctggtggtg ttggggaagg agcgatgcca ttatcgattg gttatgcagc aatattatct
     1801 ggtatgggac aaaatgatat ctttgggaag attctaccgt gtgtgatgtt gggaagttta
     1861 gtcgcagttc tactgtctgg tatgctcaaa aaaattggag agaaataccc acaattaaat
     1921 ggtcagggta atttgatcga tggggatgat gaacatttag agaacttgaa gggttcagct
     1981 aaaccagtca atattgaaaa aacattgtta gcaggcgtgg tagcaataac gatttatttt
     2041 gttgctgtct atgttgagca cttattaaaa tcactccttg gcttttctat tccttcacct
     2101 gtgatcttat tagtctctct tatgggggct aaaatgcttg gttttatccc taaaaatatc
     2161 gaagaaggtg gaaattcgtt gtatggattc actgttcgtg ggatcacacc tcccttactt
     2221 tttggcgtag gggttgcaaa aacagattgg ttagagctga tcgaagtatt taaaaatcct
     2281 gcaacgatag taacaatcat tgtgacggta gtagctattg tcgctacagc ttttatcgcc
     2341 gcaaaatttt taaaaatgta cccagttgat actgccatcg cagtttcatg ttgtagcgga
     2401 caaggcggta caggtgcttt agctatctta gctgcgggtg atcgcatgga attaatgcca
     2461 tttgcacaag ttgctgttcg actagggggt gctatgaccg taacgttcgc tatcttttta
     2521 atgggtctgc tatcataaga acataaagaa aggacgtgac catgtgtttg atcaaacaga
     2581 tatccaacga ttatttttaa aaaataacta tatcaaagac caatgggaaa atcttttgct
     2641 tgattcagga atacaaaaga agcaaatcga agattttcaa catttagatc aaacattggg
     2701 tatataccat aaagaaaagt tagtaggaac agtatcttat caaaacaacg tgattaaata
     2761 tatcgccgtg tctgaaaaat ataaagatgg tggacataca tttaatgtac tagtctctgc
     2821 aataattcag gcaatgagtc aacaagagat ttttcattat attgttttca cgaaaccgcg
     2881 ctatcaaaaa agttttaact ttttaggttt tgaaacaatc gtatctaccc caaaaatagc
     2941 tgtattagaa atgggagata gttctataaa acagtaccta gagaggattc ctaaagtgaa
     3001 tggtgaaaaa aaagcagcaa ttgtcatgaa tgcgaaccca tttacagaag ggcattatta
     3061 tttagtaaaa caagctgcca ataacaacga ttgggtatat gtatttgtac tcgaaaaaga
     3121 acaaaatctt ttttcgacga aggaccgact caaattagtt caacaaggat tacaaaaact
     3181 gaaaaatgtc attgttctat caggtggcga gtatattatt agccctgcaa cgtttcccac
     3241 ctatttttta cgagaagatg atgaaatagc aaaggagcaa atggtcgtag atgctacttt
     3301 attcaaagaa agaattgcca cttatttaga tatctctgct agatatgtgg gagaagaacc
     3361 actttcccca atgactcgtt catataacga catactggaa acgattttac cccctgaaat
     3421 aaaagttcat gtccttgcaa ggaagaaaac ggaacaacac caagttattt ctgcttctca
     3481 agttcgtaaa gcgtatcttg ctgggcaatt ggaaaaaata aaatatatgg ttccagaaac
     3541 aacctatcaa tacttaaaaa ataaaaggga gcgttaagta atggaaataa ataaatcagc
     3601 gattgctgga acattagagt caagtgatgt ccaaatcatg atttcaccga atacagaagg
     3661 aatagaaatc gatttagaca gtcaagtgat tgagcaattt ggaaaacaaa tcaaaaaagt
     3721 tatctatgac acactagcag tttatcagat taaaaacgta aaactcaagg tagtagataa
     3781 aggggcatta gattgcgtta ttgttgcacg attaaaaaca gcgatccatc gttccttagg
     3841 tatccaacac caagaaacac cgtgggaggt ttaattagat gatgaaagaa cgtttgagaa
     3901 gaacaatgat gtttgttcca ggaaataatc cagcgatgat caaagatgca ggtatttatg
     3961 gtgctgactc gattatgttc gacctggaag atgcagtgtc tatggcagaa aaagacagcg
     4021 caagaatgct tgtggctgag gcattgaaaa cagtggatta tggtgacaca gaattaattg
     4081 ttcgaataaa tgacctgcac tcagaatttg gacgtgcaga catttttgca atggttaaag
     4141 ccggcgcgca tgtattacgt ttacctaaaa cagaaacctc tgaagatata acagaagtag
     4201 aaaaagtgat tatagaagca gaaaactatc ataatgttcc gatagggcga acattgatga
     4261 tggcagccat tgaaggtgct tctggtgtat tgaacgcacc tgcgatcgcc aaagcatctg
     4321 atcgattgat tggaatcgcc attggggcag aagactatgt gacaaatatg aaaaccaaac
     4381 gatatccaga tggaaacgaa ttgttttttg ctcgcagtat gatcgttcat gctgcacgtg
     4441 cagcaggcat ttccgcattt gacactgttt actctgatgt ggatgatatg gaaggatttt
     4501 taaacgaagt acgtttaatc catcaattag gttttgatgg aaaatcttgt atcaatccac
     4561 ggcaaattcc agtagtcaat actgtttatc agccgtcaat caaagaaatc aaacgatcac
     4621 tagacatcat ctcggcaatc gaagaagcaa aagaaaaagg ttctggcgta atttctttaa
     4681 atggaaaaat gatcgataaa ccaattgtag aacgtgcaca acggacattg atgttagcaa
     4741 aagcatcacg attgattgat gaggagggaa atatccatgc ataataaaca aagacgtagc
     4801 attccatcaa gtgttttaac aaagtataaa aaagaattcc aaagtgtttc attatcgcca
     4861 caaacgattc aacgttgtgc accaaaagta acgactgtaa ctaatcagac aaataaattg
     4921 atgcactcga tcaaagaagc tatagaaaaa tcgggcttaa aagatggaat gacgatttca
     4981 tttcatcatc atttgagaga aggtgatttt gtttttaacc aagtgatggc cgtgatccgt
     5041 gaaatgggca tcaaagaatt gaccttagca ccaagttctt taacgaacac tatgaatgag
     5101 atggttattg aatgcatcaa ggacggtaca gtaactcata tcacaagcag tgggatgcgc
     5161 ggcagcttag gtgaatttat ttctcatgga ggaatggaca atcctgtgat tttacgttct
     5221 catggtaatc gtccacgtgc aattgaaagt ggtgaattga aaatcgatgt tgcatttcta
     5281 ggagtaccaa catctgatga ttatggaaat gctaatgcga ctgttggaaa atccgttttt
     5341 ggttcattag ggtatgcaaa agtagatgca caatatgcag ataaagtcat tcttctaact
     5401 gatcatttag ttccttaccc taatacgccc attagtattc cgcaaacaca agtcgactat
     5461 gtcgtggaag tagaagcaat tggtgatccg aataaaatcg gtgctggtgc tacacggttt
     5521 actaaaaacc caaaagaatt aaaaattgct gaaatggtca aagatgtcat tgttcactca
     5581 ccttatttca aagatggctt ttcttttcaa acaggaacgg gtggtgcagc tttagcagta
     5641 acgcgttact tagctagtga aatggaagaa aaacagatca aagcttcttt tgcattaggt
     5701 ggcatcacta aaccaacggt tgatttattg gaaaaaggtc ttgttgaaaa agtgttagat
     5761 gtccaagact ttgacaaagg agcagctgaa ggaatggcgc ggcttgagaa ccaacaggag
     5821 atcgatgctt ccttttatgc tgatccaagt aataaaggag cggttgttaa ccaattagat
     5881 gtcgctattt tatctgcatt agaaattgac acaaacttca atgtaaatgt tatgactggt
     5941 tcagatggtg tgctaagagg tgcgattggt ggacatcaag atgcagcaac ggctaaactt
     6001 actatcatct cagcgccgtt agtacgtggc cgaataccta cagtagtaaa tgatgttact
     6061 acattgatca caccagggaa aagtatcgat gtattagtca cagaagtagg ggttgctatc
     6121 aatccccaac gtaaagatct aatagctatt tttgaaagaa ttccacaaat tcctgttttt
     6181 acaatagaag aattacagca aaaagcagaa aaaatagtag gcatcccgga acctttgcaa
     6241 ttcacagatc ggatagttgc ctatgtagaa taccgagatg gttcaatttt agatgtcgtt
     6301 cgtcaggtga aagaatttga agaagagaga tcatgatgtt tgatactgga gaaattcaag
     6361 aaatcgagga tattttgatc cataaagaac aacgtgctaa cttgcaaagc aacctgcttt
     6421 ccaagtattc aaatagttca ttgatggtct ttacattgaa tattcctggg ccgatcaaaa
     6481 ataatccaat gattgagcag gtatttcgtc agggacagca gtcaattcag caaatgtttt
     6541 taaaaaaaaa ggtggaaatg acgtacacaa aagaaatcaa tttgccaagt ggattggact
     6601 ggtacgtaat agtaacagaa gcacctttga atctcaaaaa atggttggtc acgttagaag
     6661 agcaagacgc attaggtaga ctttttgatt tagatgtctt gttcaaaaaa ggagagcaga
     6721 taacaattgt cagtcgccaa gctcttaatc atggggaacg tccttgtttt atttgtggga
     6781 aatcagcaaa ggtatgtgca cgtaatcgta cacatactgt gaaagaaatg caaaagaaaa
     6841 tcgaacaaat agtagccgat tccttaaaag gagcattgaa acatgcatga taatattcaa
     6901 aaaatagcac aagaagcaca attagcgatg ctttatgaag ttacatgtat caataaacca
     6961 ggattggttg atccagtaga ccccgggtct catcaagata tggacatttt tacttttcta
     7021 caaagtagtg tagttttgac tccctatttt gaagaattta tccaaacagg attggaccga
     7081 caacatcaac caattgaaga aacctttatg gcgattcgac agattggttt agaggctgaa
     7141 actgcgatgt tctcagtgac aaatggtata aatacacata aaggtgctat cttttcattg
     7201 gggatttttc ttgctatttg tggccggtta actatttgga aagttccttg taaaaaaagt
     7261 ttctttcaaa aagaaattca aaaaatgacg aaaaatctac ttaacgattt tgggaagatt
     7321 gatcaaacaa aaccaaaagc atggacttgg ggagaatatc tatttgttaa ccatggattg
     7381 acagggatac gaggggaagc caaaagaggt tatccttgtg tttttgaccg aggattgcct
     7441 tattatcaag catatcaagg gtcgcaacaa gataagatga tagacacttt actttttcta
     7501 tcaatggaag tagaagatac caatttgatc aaacgaagta ataatagaca tgtttttgaa
     7561 gagtatcaat tacttattcg tccttatttt gaattagggg gtgtaaaaac catacaaggt
     7621 aaaaaatatt tggattacct aaatcagcaa tttaaaaaga aaaattggag tattggtgga
     7681 agtgcagact tattgatcct tacaatcttt ttagataaaa tcatggacaa aggatggtta
     7741 tgttgatact ataatacatt attttgtatt agtaagctaa taactaaaaa atggttcaat
     7801 acaaatattt tataaaaaat ggtagtgcct atcctttcag attgtagaca aagaaaaatt
     7861 tctttgtcta caattttttt gttaatgact tcagtcagtt tcgtgcgcaa agcgtgcgaa
     7921 acaagaaaac catgcgtttc tacacgtgga agaactagca tatcgaaagg agttttttca
     7981 tgtcaaatga cgataaaagt ttagcccata ctcgttggaa ttttaagtat catattgtat
     8041 ttacaccaaa atatcgacga aaagtaatct ataggcaatt acgaaaagat attggaaaaa
     8101 tacttaggaa gttgtgcaaa ataaaaaacg tggaaattat agaagcacat gcaacgccag
     8161 atcatattca catgcttgtt caagtaccat caaaaataag tatatccagt tttatagtta
     8221 tctgaaagat cgtagtgctg tgctaatcca cgagcagcac gtaaatcaca aatatagata
     8281 cggaaataaa tcattttggt cggaggaata ttatgtgagt acagtcgaat tgaatcaaaa
     8341 gacaattgaa aaatacatcc gtgaacaaga agctgatgat cgcatttgag atagtataag
     8401 taaaagagag tagatgttgc aataacaaaa gggtaaaaat ggactacctc cagtaaaata
     8461 ccgaaaaata gtttaacttt tggagataag tccatttaag ggtctataga atgacaattt
     8521 ttttagggga ttttactatt attacttcaa gacccgcttg gattcgcaaa gtgagtgaac
     8581 tcttaaaact acctgatagt aggttgtttt aagagttatc tgaaagtaaa aattgcattt
     8641 atatttttga taaatacgta aatttatata tacgtattta tcaaagtttt aggccaaagg
     8701 aatattgtgt aaatacacta ggattgaatg aatagaccag aagaaattgc gtccaagaac
     8761 aaaagaagca tgatagtgct ctatataaat tgggcattca agaatctgaa atttaaaggt
     8821 agaattttag ttagactttt agattttaaa cacaaaaata accatacaag caacaagaag
     8881 caaagtgcta tattataaat tgtccgtccg tttacttaca taagatcatt ggggctattt
     8941 ttaaaagaga ataatagata ataaatgaaa aaaaagtcaa gtccacaatc ctgtgtaaaa
     9001 tactatacaa tgctctcact attttttact tggcaaattt aatatatttt cttgatgaag
     9061 aaagccactc atcgtgttga tcaataagga ctgctccaat taaacgattg gctgaaacgc
     9121 agtttggaaa gatacgaata attttttctc ttctgcg
//
LOCUS       DACEJZ010000103         8969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEJZ010000103 DACEJZ010000000
VERSION     DACEJZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.106"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(57..350)
                     /locus_tag="IW656_002438"
     CDS             complement(57..350)
                     /locus_tag="IW656_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0838122.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="IW656_002439"
     CDS             complement(378..1043)
                     /locus_tag="IW656_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0838123.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="IW656_002440"
     CDS             complement(1183..1815)
                     /locus_tag="IW656_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0838124.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="IW656_002441"
     CDS             complement(1822..2889)
                     /locus_tag="IW656_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0838125.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="IW656_002442"
     CDS             complement(2886..3617)
                     /locus_tag="IW656_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0838126.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW656_002443"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="IW656_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0838127.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW656_002444"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="IW656_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0838128.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="IW656_002445"
     CDS             5780..6949
                     /locus_tag="IW656_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0838129.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7216..8838)
                     /locus_tag="IW656_002446"
     CDS             complement(7216..8838)
                     /locus_tag="IW656_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838130.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1701 c         1333 g         3079 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     7501 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     7561 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     7621 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     7681 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     7741 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
     7801 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
     7861 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata ttatacccat
     7921 tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta gtataattaa
     7981 acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta tacttccaat
     8041 caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat aaggtctatt
     8101 cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg aaagaagtaa
     8161 agtaactata gcagctttta ttaatttaat aattaagaag atttctattt tctttttccg
     8221 ataaaattga ataattaaag caattataat cattaccata gtcatagcta cagaaagaac
     8281 atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt ctttttttga
     8341 gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg cgagtaattc
     8401 ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg tatatataat
     8461 agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat aatgacatat
     8521 aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg ctaaattagt
     8581 aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat tatttacttg
     8641 catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag attctaaact
     8701 taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca tgtttttaaa
     8761 tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt ttacagaaaa
     8821 aatttttttc aaattcatat accacctcat gtttttttat attacatata gtatcattta
     8881 tttttttaaa acactagatt agtcactata taaaatgtga ttaattttat tattaataaa
     8941 atatttttta tattacaaaa gtagtgttt
//
LOCUS       DACEJZ010000104         8818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEJZ010000104 DACEJZ010000000
VERSION     DACEJZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.107"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..807)
                     /locus_tag="IW656_002447"
     CDS             complement(310..807)
                     /locus_tag="IW656_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0838131.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IW656_002448"
     CDS             complement(847..1656)
                     /locus_tag="IW656_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0838132.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IW656_002449"
     CDS             1990..2847
                     /locus_tag="IW656_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0838133.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IW656_002450"
     CDS             2840..3742
                     /locus_tag="IW656_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0838134.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IW656_002451"
     CDS             3775..4125
                     /locus_tag="IW656_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0838135.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IW656_002452"
     CDS             4159..5097
                     /locus_tag="IW656_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0838136.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IW656_002453"
     CDS             5175..6068
                     /locus_tag="IW656_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0838137.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IW656_002454"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IW656_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0838138.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IW656_002455"
     CDS             6683..6871
                     /locus_tag="IW656_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0838139.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IW656_002456"
     CDS             6884..7786
                     /locus_tag="IW656_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0838140.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IW656_002457"
     CDS             7818..8060
                     /locus_tag="IW656_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0838141.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IW656_002458"
     CDS             complement(8316..8687)
                     /locus_tag="IW656_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838142.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3073 a         1522 c         1706 g         2517 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DACEJZ010000105         8764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEJZ010000105 DACEJZ010000000
VERSION     DACEJZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.108"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(45..1511)
                     /locus_tag="IW656_002459"
     CDS             complement(45..1511)
                     /locus_tag="IW656_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0838143.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IW656_002460"
     CDS             1898..2494
                     /locus_tag="IW656_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0838144.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IW656_002461"
     CDS             complement(2552..3034)
                     /locus_tag="IW656_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838145.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IW656_002462"
     CDS             complement(3140..3337)
                     /locus_tag="IW656_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838146.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IW656_002463"
     CDS             3632..4390
                     /locus_tag="IW656_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0838147.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IW656_002464"
     CDS             4450..4878
                     /locus_tag="IW656_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838148.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IW656_002465"
     tRNA            4924..4996
                     /locus_tag="IW656_002465"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IW656_002466"
     CDS             complement(5146..5517)
                     /locus_tag="IW656_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0838149.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IW656_002467"
     CDS             5732..5917
                     /locus_tag="IW656_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838150.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IW656_002468"
     CDS             complement(6000..6188)
                     /locus_tag="IW656_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838151.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IW656_002469"
     CDS             complement(6212..6475)
                     /locus_tag="IW656_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838152.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IW656_002470"
     CDS             6633..6890
                     /locus_tag="IW656_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0838153.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IW656_002471"
     CDS             complement(6961..7152)
                     /locus_tag="IW656_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838154.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IW656_002472"
     CDS             complement(7293..7613)
                     /locus_tag="IW656_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838155.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IW656_002473"
     CDS             complement(7636..8070)
                     /locus_tag="IW656_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0838156.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8764
                     /locus_tag="IW656_002474"
     CDS             8405..>8764
                     /locus_tag="IW656_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0838157.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAA"
BASE COUNT         2782 a         1742 c         1395 g         2845 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattcaatt tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga
     8641 aggctctgca caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc
     8701 tgaacctgtt caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc
     8761 agcg
//
LOCUS       DACEJZ010000106         8663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEJZ010000106 DACEJZ010000000
VERSION     DACEJZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.109"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IW656_002475"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IW656_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0838158.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IW656_002476"
     CDS             complement(740..1462)
                     /locus_tag="IW656_002476"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0838159.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IW656_002477"
     CDS             complement(1598..2479)
                     /locus_tag="IW656_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0838160.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW656_002478"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW656_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0838161.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IW656_002479"
     CDS             3719..4198
                     /locus_tag="IW656_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0838162.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IW656_002480"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IW656_002480"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0838163.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IW656_002481"
     CDS             5499..5627
                     /locus_tag="IW656_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0838164.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IW656_002482"
     CDS             5631..6176
                     /locus_tag="IW656_002482"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0838165.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6630..8321)
                     /locus_tag="IW656_002483"
     CDS             complement(6630..8321)
                     /locus_tag="IW656_002483"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0838166.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2411 a         1773 c         1568 g         2911 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt tccaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tcataaaaaa aaa
//
LOCUS       DACEJZ010000107         8619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEJZ010000107 DACEJZ010000000
VERSION     DACEJZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.110"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(196..1149)
                     /gene="menA"
                     /locus_tag="IW656_002484"
     CDS             complement(196..1149)
                     /gene="menA"
                     /locus_tag="IW656_002484"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0838167.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1162..2265)
                     /locus_tag="IW656_002485"
     CDS             complement(1162..2265)
                     /locus_tag="IW656_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0838168.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2355..3290)
                     /locus_tag="IW656_002486"
     CDS             complement(2355..3290)
                     /locus_tag="IW656_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0838169.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3592..5526
                     /locus_tag="IW656_002487"
     CDS             3592..5526
                     /locus_tag="IW656_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0838170.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5983..6399
                     /locus_tag="IW656_002488"
     CDS             5983..6399
                     /locus_tag="IW656_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0838171.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6413..6979
                     /locus_tag="IW656_002489"
     CDS             6413..6979
                     /locus_tag="IW656_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0838172.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7002..7988
                     /locus_tag="IW656_002490"
     CDS             7002..7988
                     /locus_tag="IW656_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0838173.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8143..>8619)
                     /gene="scm"
                     /locus_tag="IW656_002491"
     CDS             complement(8143..>8619)
                     /gene="scm"
                     /locus_tag="IW656_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0838174.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2537 a         1667 c         1743 g         2672 t
ORIGIN      
        1 tggaaagcca ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac
       61 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
      121 atataaggag ctatttttat ttggctgttc ttattacttg atgcagggca gttttttcac
      181 aacctcttca tgctgttaaa tggcttgcat aatgattgtt agtaatagcc ccaatacata
      241 actggcattg aataagacca tatttttgat ggaatagctg aaactgatag gctggggtaa
      301 cgtttgcttg aattgatcca aatgcttctt cacaattggc aaggtcaaga aggtgaccaa
      361 gatcggccat tcatagatac cgctcaacaa ccccattaaa atcacgaaat agcaagcata
      421 catcaatagc tggaataaga ctgttcccgc aggacgtccg atataaaaaa ctagtgtata
      481 gcgatgattt tcaatatcac ggtcaagatc ccgcaaatta ttagccaaca tgatattggc
      541 aatcgtaaac accaagggta aggaagctaa tacgattgct gctatagccc aaagatttcc
      601 taccaaatca aattgtccag atgaaaagtt gactgtcaga tcatagacat ctgaaccgaa
      661 tgtattgaca taaacgacca tggcaaaaat tccaaggccc atagtcaccc cactgaaaat
      721 ttcccctaaa ggcattcgag ataaaggaat cggtccgaat gtatagaata ctccaataaa
      781 acaacagata aatcccatca aaagtaaaag ccaacccgta ttgatcgtta gaataacgcc
      841 gataacaaaa acaaatgcaa tcatagctaa aatcatgttt cgaatcaata caggagatag
      901 atttctgcgg ccaatgacat tttcttcata cttatatact tcagaatgag ccttttcata
      961 atccatgtaa ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa
     1021 taaaaaagtg tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac
     1081 cgcaaaagga aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt
     1141 tagtgtcatg gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga
     1201 gtcctccgat aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt
     1261 tgtcacgaag atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt
     1321 tttcacaccc attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt
     1381 tatgatcgtg acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa
     1441 atgatggtat tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa
     1501 tgttttgttc cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg
     1561 gatgccgact gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt
     1621 accgcccaag tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc
     1681 gtccgtaatg taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag
     1741 ataaacagtt ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc
     1801 ctgatcgatt tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg
     1861 tgtgattgca ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc
     1921 ccgcttgact aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc
     1981 attcacttca tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt
     2041 tactcgatcg aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg
     2101 aacgtaagtc cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc
     2161 tgctgaactt tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa
     2221 agagatgccc agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc
     2281 attttgaaag gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta
     2341 gtatgattat gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg
     2401 cctttttcag ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt
     2461 gtagcacctg taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg
     2521 acgaattcgt cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc
     2581 atattttcat tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca
     2641 aagtttgatt cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg
     2701 ttagagtagt tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg
     2761 atttcgattg tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt
     2821 tggaagcttt cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt
     2881 gcttttaaga attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt
     2941 gcttccatgt ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg
     3001 ttatcatatt ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg
     3061 taaccatttg aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca
     3121 gctacaactt ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat
     3181 gattcagaag aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga
     3241 gctgaaaatg ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc
     3301 caccattcca tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt
     3361 tagcctataa gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt
     3421 cagcaaattt cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta
     3481 gacaaaagaa cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa
     3541 agaattatca tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag
     3601 caaaatattg tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca
     3661 aagaaactga aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact
     3721 atgatgaccg agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac
     3781 gatctgcagc atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc
     3841 ggtatcgata aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag
     3901 ctgatccttg gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac
     3961 ggattcaccc tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca
     4021 gtagcaaaag ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt
     4081 gtctgtggtt ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat
     4141 cgcctagcca aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca
     4201 atgccgacga tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg
     4261 cgtaagcaca acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat
     4321 atcaaattga aagacggacg caacattccg actcacacac tgatttggac agctggtgtc
     4381 aaaggaactt ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc
     4441 gccaatgaat atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat
     4501 ttagtctttt atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa
     4561 caaacaggac acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat
     4621 aaatttaaag gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct
     4681 aacctatttg ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg
     4741 aatttgaaat acttctttga tattcgttct ggttattata tgttccaata tttaatgcat
     4801 gagtttttcc atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc
     4861 aatgtcttat ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct
     4921 tccaaaaaaa tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga
     4981 tcatggttca ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt
     5041 gccagtggtg cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt
     5101 gccggagaag cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa
     5161 ccaatggctg tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg
     5221 ccaaacaatg atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg
     5281 gcattagcat tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg
     5341 gtgatcacgt tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt
     5401 gctttcgcat tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca
     5461 tggctacaac gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag
     5521 aaataaatag ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca
     5581 aaccgtcaca gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc
     5641 catgagcaag acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc
     5701 agaagtagct tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg
     5761 taaattcctt cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg
     5821 tgttccacct gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt
     5881 ttctattctt cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta
     5941 tgctaaaatt gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt
     6001 gctgaaaaaa gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt
     6061 tcttttttac cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa
     6121 gcggtactgc gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag
     6181 agcaacacat atacttacaa agatgaacac ggagattata acttgatcga gatagatggc
     6241 gatcgagtac ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg
     6301 gcaacgaaaa atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt
     6361 tcaacagatg gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa
     6421 attacaaaaa atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt
     6481 ggaaaatatg atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa
     6541 tctaatcacg atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg
     6601 gctacgacta ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat
     6661 gagtggtgct ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg
     6721 tgtcagtatc atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt
     6781 gatggcttct ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt
     6841 tttagggatt ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt
     6901 gcagacactt caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc
     6961 aatgtttacc aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt
     7021 gggaaaacta tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa
     7081 ctgtccgcct gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg
     7141 gaaagctgtt gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc
     7201 gagacaaagc catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc
     7261 atgatgatat cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt
     7321 ttggtaatgt cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat
     7381 tgtctgatta ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag
     7441 tattgaatgg cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag
     7501 aatatttgga aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg
     7561 gcgcttttga aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt
     7621 ctatcgggat ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg
     7681 aaatcggcaa acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct
     7741 actctttgca aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga
     7801 cagaagaaga tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag
     7861 ctaggaagtt agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg
     7921 ccacctctct tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca
     7981 aagactgaat agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt
     8041 tttttgacaa attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa
     8101 cgtccgttag cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt
     8161 ataactcata aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat
     8221 ccaattattc gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc
     8281 attgcttgtt ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa
     8341 ggagacactt gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga
     8401 agttgatgaa tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc
     8461 tgacgtgtcc gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga
     8521 ggtagtatct gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga
     8581 tgtatccgta ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DACEJZ010000108         8607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEJZ010000108 DACEJZ010000000
VERSION     DACEJZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.111"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            287..694
                     /locus_tag="IW656_002492"
     CDS             287..694
                     /locus_tag="IW656_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838175.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IW656_002493"
     CDS             708..1109
                     /locus_tag="IW656_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838176.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1296
                     /locus_tag="IW656_002494"
     CDS             1111..1296
                     /locus_tag="IW656_002494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838177.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYICLFDYLFISS"
     gene            1253..1414
                     /locus_tag="IW656_002495"
                     /pseudo
     CDS             1253..1414
                     /locus_tag="IW656_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(1420..2613)
                     /locus_tag="IW656_002496"
     CDS             complement(1420..2613)
                     /locus_tag="IW656_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0838178.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFQNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSK
                     ETIQAFQRMMKKRPKAETFEVDGRSNFLFVNHKGKPKVAIDYSTLFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2692..2895)
                     /locus_tag="IW656_002497"
     CDS             complement(2692..2895)
                     /locus_tag="IW656_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ0838179.1"
                     /translation="MNNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2895..3986)
                     /locus_tag="IW656_002498"
     CDS             complement(2895..3986)
                     /locus_tag="IW656_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0838180.1"
                     /translation="MTTHKNLSVKIDYISIVFETATAEDVIMHILDLPTDIFNVYPAT
                     IKFKTYQARWQIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGD
                     MFRRCERRYGLDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDES
                     KFDDFDTAKTVYIGAGKSGLSYRFYDKDKEVCSKHNKTLDEVGSWKRTEMQLRDDKAH
                     AFAMTFKDRPLELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAVEVLKL
                     QVPKLHNSLEETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSTSLSS
                     KLTAHLQRINRTDLIPYIQYDTKHGKGGI"
     gene            4453..5124
                     /locus_tag="IW656_002499"
     CDS             4453..5124
                     /locus_tag="IW656_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838181.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNYPTFDTLEKIMDALGVTPNELLSGEWKYIDHTEPYIMDIIKREQDFNVSLDYLL
                     DNEFFNDEKEYKFYMETILIRYISNYLSNEVVEIEELMEIKQLIQRQKIERMMKVHKE
                     MRGLDRYREQPKEYKYHDPYDDWIFRQLADIDNRKNIPNTSPQVDFNEADYEDYLKAK
                     WNRGL"
     gene            5349..5654
                     /locus_tag="IW656_002500"
     CDS             5349..5654
                     /locus_tag="IW656_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838182.1"
                     /translation="MNDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVNARQEGKWTHYSLSKRGSEYASKRLLELTTPNTENQSNCCK"
     gene            5708..6745
                     /locus_tag="IW656_002501"
     CDS             5708..6745
                     /locus_tag="IW656_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0838183.1"
                     /translation="MLYKGGTFLEVVNSIWLFFQDQILGMKWLNGLIGNGLSQLGLDI
                     DSRLGGSIQFFLYDVIKITILLCLLIFFISYIQSYFPPERSKKILGRFHGIGANIISA
                     LLGTVTPFCSCSSIPLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGAKVAF
                     AYVIIGLIIAVIGGTLIEKMHMEKYVEDFVKNASNVDISSPTLTKKDRVQYAKEQVIG
                     TFKKVFPYILIGVGIGSIIHNWIPETWIESILGSNNPFGVILATLVGIPMYADIFGTI
                     PVAEALLAKGAQLGTILSFMMAVTTLSLPSLIMLKKAVKSKLLTLFIAICTFGIIIVG
                     YLFNIFSTLFI"
     gene            6762..7094
                     /locus_tag="IW656_002502"
     CDS             6762..7094
                     /locus_tag="IW656_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0838184.1"
                     /translation="MKLFGKKKETKSCCCGGNCTSETMQNAENKKQEQGIKILGSGCA
                     KCMELEKATRTAISELQLDYEIEHITDFAEIASYGVMSTPALVLNGEVISYGKVLTAD
                     EVKALLSK"
     gene            7124..7546
                     /locus_tag="IW656_002503"
     CDS             7124..7546
                     /locus_tag="IW656_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HAQ0838185.1"
                     /translation="MKKPKVAFVCVHNSCRSQIAEALGKHLSSNVFESYSAGTETKPQ
                     LNQDAVRLMKEMYQIDMEKAQYSKLLSEIPQVDIVITMGCNVNCPVIPCEYREDWGLS
                     DPTGKNDVEFSKIIHSIHTKILELTEKIKNNELISRRN"
     gene            7749..8189
                     /locus_tag="IW656_002504"
     CDS             7749..8189
                     /locus_tag="IW656_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0838186.1"
                     /translation="MRKGGEIVNTSSFEHIVRIQFNALMITVIKCTVKNRNREFARRS
                     KREVLFCELSDVKQLECGTTDNYSCDYTSFEVLNTPIQVSNEKLSIALHKLSIKERDV
                     ILLRYFQGMSDQEIAELYHVSRSAIYRRRSNGLNKLKTLLKERN"
     gene            8194..8433
                     /locus_tag="IW656_002505"
     CDS             8194..8433
                     /locus_tag="IW656_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0838187.1"
                     /translation="MKKEYGYPSLDVICKASAGNETAIKEILNFYDAYICKLCLRPFY
                     HSENGKITMQVDEELKSLIHAEMMKAILKFEIRVK"
BASE COUNT         2729 a         1440 c         1623 g         2815 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 atatgtcttt ttgactatct ttttatatct tcttaaatgt ccgtaaagcc ttattttatc
     1321 ggctctacgg gcattttgct tttgtggtaa aactcacata tctaggtcta tcttcttata
     1381 ttttcgctat caagcgtggt taaaatcgtg gtaaatactt catgcgatta gacgctgaac
     1441 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
     1501 tgaatgtccc atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccagccttgt
     1561 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aaggggttat ccttatggtg
     1621 cttgttgtat ttctttacca tacggacaaa taaagtgctg taatcaatgg caactttggg
     1681 cttgccttta tgattgacaa ataagaaatt gctccgtccg tcaacttcaa atgtttctgc
     1741 ctttgggcgt ttcttcatca ttcgttgaaa tgcctgtatc gtttctttgc ttaatggtac
     1801 ttgccttgtt ccactttttg tcttaggtgt ttcaatgtaa tagccctgtt ccttgctttt
     1861 taataactgg tggtcgataa tcacaacttc attttgaaaa tcaatatcgg ctactgtcag
     1921 tccgcacagt tccgagatac gaagtcctgt ctttaacagt atcagcacat catcataata
     1981 cttgtgatac acattgtccg tcttgatgaa tgagagtagg gcttgttcct gttcctctgt
     2041 caatgcgact ttctctttgg tatcattttc taggacttca cttaatttga aatcaaaagg
     2101 gttcttcctc acacaatcgt cttgtatggc gatatagaat gacgctttta acgagcgttt
     2161 atggttgtta atggtattgt aggaaaagcc tttatctttc atgcgtaacg cccattcttt
     2221 agcgtcagag ggttttatcg tatcaatgct cctagcacct aacttgtcct cttttagtaa
     2281 tcgcatgagc tgttcccgtt gtttctgtgt gcttttcttc acgtttgccc gttgtgcgtt
     2341 ctgtttggcg tagagttggc aaagtgtcat tttcttgcct gtgctgtcga taccgtcctc
     2401 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gtttcccctt
     2461 tggtgtcggg tctgtgggtg ttaatctcca agcatacaca tattttgtgt ttccaaatgc
     2521 gtccacatat ttatataagt attttccatc tgttcgttgg ctctctccag tatgcaggat
     2581 acgtcctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
     2641 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagattgc
     2701 gtccaattta tcaatgacct tttcaaactg tcggcgttta atctgtatgc ggttgccatt
     2761 cataatgagc cagccagcgt ccttgttttc ctctgccaag cgtcttaact tgttttctcc
     2821 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
     2881 atcgttgtta ttcatcagat accccctttc ccatgtttcg tgtcatactg aatatagggg
     2941 ataaggtcgg tgcggtttat cctctgtaag tgggcggtta gtttactgga aagggaagtg
     3001 ctgtatcttg ccttatcaag catagttccc actttttcca gtccacctaa tgcgtcatgt
     3061 tcttccaaga agtaaaaact tttcacagcg gaaatcacgc caccctctgt gagccattgc
     3121 tgtgtttcct caagagaatt atgtagtttc ggtacttgca gttttaagac ttccacagcc
     3181 cctaaaaagc gttcccaaaa ccgacacgtt ttccatctgc tcttattact ttcatttctg
     3241 tttggcacga caaagcgtag gttgtttgcc aatagcccga aagccagttc cccaagttcc
     3301 agcggtctgt ccttgaatgt catggcaaaa gcatgagcct tatcatcacg cagttgcatt
     3361 tctgtccgtt tccaactgcc gacttcatca agtgtcttat tatgttttga acagacttcc
     3421 ttatctttat cataaaagcg gtaggacaat cccgatttac cagcaccgat ataaacagtc
     3481 tttgcggtgt cgaaatcatc aaacttgctt tcgtcaaagt ggtagccctc actattcgag
     3541 ataaattcct ctttttcgca tttcttcttt atctgctcta tggtaaagaa tggcttttcg
     3601 ttcttatcat caatggcaat atcaagtctt gtgaaatgga aattatccag cccgtatctt
     3661 cgctcacagc gtcggaacat atccccaaag gtataattcc tactgtcgag aatacggaaa
     3721 atatcatcac aacctctgcc agtcataaca agataacagc ctagcccttg tgggttgtct
     3781 tctgtctttc ttgcgtcccc cgatacataa atatctccaa tctgccagcg tgcttgataa
     3841 gtcttgaatt ttatcgttgc tggataaaca ttgaaaatgt cagtcggtaa atctaaaatg
     3901 tgcatgatta catcttcagc ggttgccgtt tcaaatacaa tgctgatgta atcaatctta
     3961 acggataaat ttttgtgtgt agttatagtg catttctcct ttcgtatgcc ccgttttttt
     4021 gcggggtaaa aattgtgttt tttcctttat ttatcacagt ttctaggtac tatgacatgt
     4081 atacgcaggg cggtgttaca tatacgccct atgcgttcgc ctaaaggtgt tccctttctt
     4141 cctgtgtctg ccccgttctt aacgctcaca agccttgtaa ggctcgtggc taaacggaac
     4201 agccccagtc agaaacctta ttttcggtct gatagtccat gcttattcat gctatcaaag
     4261 tgaaaataca tcttttctat gcttactcaa gcgtgtgatt gaccctagcg tgcttaatca
     4321 ctctatctaa cacgataata gcgccattag aattacttgt caagaagttt ttgttgactt
     4381 tttgatatat attaagtatg atatgcttat aaataccaag aaaagcaatg aaaggagtgt
     4441 ttatactaag tcatggagca ttatgaaaag aaaatcagtt tcttaggaga gaacatacag
     4501 accataagga aacacagagg aatgaaacaa caagaacttg cggacaaaat aggtatcaat
     4561 atgcagagcc tttccaagat tgaacgaggt gtgaattatc ctacctttga cacattagaa
     4621 aagataatgg acgcgctggg agtaacaccc aatgaattat tatcgggaga atggaagtat
     4681 attgaccaca ccgagcccta tatcatggat attatcaaac gggaacagga cttcaatgtt
     4741 tctttagatt atctgttgga taatgaattt ttcaatgatg aaaaagaata caaattttac
     4801 atggaaacca tattgattag atacatcagc aattatcttt ctaatgaggt tgtagaaatt
     4861 gaagaactta tggaaatcaa acagttgata cagcgtcaaa aaatagaacg tatgatgaaa
     4921 gtacacaagg aaatgcgagg gttagaccga tacagagaac agcccaaaga gtataaatat
     4981 cacgaccctt atgatgactg gatattccgt caacttgcgg atattgacaa cagaaaaaac
     5041 attccgaata cttctccaca agtagacttt aatgaggcag actatgaaga ttatctaaaa
     5101 gcaaaatgga acagaggact ttaatttcct cttgcatgga agatacagca aacaaaaagc
     5161 ccagtaaaga gtgcaaagca ccaccaatgg tggcaaggct cttgacaggg ctttttcttt
     5221 tctgttagaa atggacaaga ggaagaaata agaatatatc aaaatatttt gatatgatat
     5281 attgacaaac tgccttatat ggcttatgat gtatatatca aaataatttg atgtgaggag
     5341 tgataacaat gaatgatttt tctaaagacg caaaaatttt taaggctctt tgtgatacaa
     5401 aaaggcttac tattttagac tacttaaaga gtggggaaaa atgtgcgtgt gttctgattg
     5461 aaaacatgaa cataggacaa tcagctttat cttatcacat gaaaatactt tgcgactctg
     5521 gtattgttaa tgcaagacaa gagggtaaat ggacgcatta cagcctaagt aaaagaggaa
     5581 gtgaatatgc gtcaaaacga ttgttagaat taacaacacc aaatactgaa aatcaatcaa
     5641 attgttgcaa ataaaggtca tcgaaaggtg gcttttataa aactctatca catcaaaaaa
     5701 aattgatatg ttgtataaag gagggacttt tttggaagta gtaaattcaa tatggttatt
     5761 tttccaagac caaatattag gtatgaaatg gttaaatggt ttaatcggta atggactatc
     5821 acaactagga ttagacattg acagtcgatt aggtggaagt attcaattct ttttgtatga
     5881 tgtaatcaaa atcacaattt tgttatgttt actgattttc tttatttcgt atattcaaag
     5941 ttactttccg ccagaacgaa gtaagaaaat tttaggacgt tttcatggta taggagcaaa
     6001 tattatatcc gcattgttag gaacagttac accattttgc tcatgttctt ctattccgtt
     6061 atttataggc tttactagtg caggactacc actaggagta actttttcat ttttgatttc
     6121 ttctcctatg gttgatttag gaagtcttgt attgctaatg agtattttcg gtgctaaggt
     6181 cgcttttgct tatgtaatta tcggtttgat aattgctgtg attggcggta ctttaattga
     6241 aaaaatgcac atggaaaaat atgtggaaga ttttgtcaaa aatgcaagta acgttgatat
     6301 tagttcccct accttaacca aaaaggacag agttcaatat gcaaaagaac aagtgatagg
     6361 aacattcaaa aaagtatttc catacatttt aataggtgta gggattggat caattatcca
     6421 taattggata cctgaaacat ggattgaaag tattttagga agtaataacc cgtttggtgt
     6481 tattttagcc actcttgtcg gtattcctat gtatgctgat atttttggaa caataccagt
     6541 tgcagaggca ttacttgcaa agggggcaca attaggcaca attttatcat ttatgatggc
     6601 tgtaacaaca ttaagtttac cgtctttaat catgctaaaa aaagcagtaa aatctaaatt
     6661 attgacctta tttattgcca tttgtacgtt tggtattatt attgtaggtt atctctttaa
     6721 tatttttagc acactattta tttaaaagga gcgaataaga aatgaaatta tttggaaaga
     6781 aaaaagaaac aaaatcatgc tgttgtggtg gaaattgcac atcagaaaca atgcaaaatg
     6841 cagaaaacaa gaaacaagag caaggcatta aaatattagg ttcgggctgt gcaaaatgta
     6901 tggaattgga aaaagcaact agaacagcaa tatctgaact tcaattagat tatgagattg
     6961 aacacattac agattttgca gaaatcgcca gttatggagt aatgtctaca cctgcattgg
     7021 tgttgaatgg cgaagtaata tcctatggaa aagtattgac cgcagacgaa gtgaaagcac
     7081 tattgagtaa gtaataaaac gaagaaaggt cggtaattta tttatgaaaa aacctaaagt
     7141 ggcttttgtc tgtgttcata attcttgtcg tagtcaaatt gcggaggcat tgggaaagca
     7201 cctttcttca aatgtatttg aaagctattc cgctggaaca gaaacgaaac cacaactcaa
     7261 tcaagacgca gttcgtttaa tgaaagaaat gtatcaaata gacatggaaa aagcacaata
     7321 ttccaaactg ttgtcagaaa taccgcaagt agatattgtg ataacaatgg ggtgtaatgt
     7381 aaattgtcct gtaattccat gcgaatacag agaggattgg ggattaagtg accctactgg
     7441 aaaaaatgat gttgagtttt caaaaataat ccactcaatc catacgaaaa tacttgaatt
     7501 gacagagaaa ataaaaaata atgagttgat ttcaaggaga aattaaatga aaatattaac
     7561 gaacagcatt gtcataaatc gttttaagca taccattaaa caatttaata tggcagttat
     7621 cagcatctga atataaggaa attttaaagg ctcgtgcaga ctatatgttt tgtgcgggct
     7681 tttttcttgc cttccttttt acattactaa aaggtataga cggtattccc gtttatatat
     7741 ttcagcgtat gcggaaaggg ggtgagattg tgaacacatc ttctttcgag catatcgtca
     7801 gaatacagtt taatgccttg atgataactg ttattaaatg cacagtaaaa aacaggaaca
     7861 gagagtttgc aagacgttct aaacgtgaag ttttattttg tgaattatcc gatgtcaaac
     7921 agcttgaatg tggaacaaca gataactatt cttgcgacta cacatcattt gaagtcttaa
     7981 atactccaat ccaagtgtca aacgaaaaac tttctattgc tttacataag ttgtcgatta
     8041 aagaacgtga tgtaatttta ttacgctatt ttcaaggtat gagcgaccaa gaaattgcag
     8101 aattatatca tgtatcacgt tctgctattt atcgcagaag aagtaacgga ttgaacaaac
     8161 ttaaaacatt attgaaagaa aggaactgaa ccaatgaaaa aagaatatgg ctacccctca
     8221 ttagatgtaa tatgtaaagc gtctgctggg aatgaaactg caataaagga aattttgaat
     8281 ttctatgacg cttatatctg taaattgtgc ttgcgtccat tttaccactc cgaaaatggt
     8341 aaaatcacta tgcaagttga tgaagaatta aaaagtctaa ttcatgcaga aatgatgaaa
     8401 gcaatcttga agtttgaaat cagagtgaag taatcatatt acatgaatga aaaatgggag
     8461 atacacagat attttgtgct atctcccatg ttgtttttag tgtcatatca aggctcattc
     8521 aatcgtggta aattagtggt aaaatgagtg tttttctata cttaaaaatg cttgaaagta
     8581 tagtgtttat gcggataatc gaaaatt
//
LOCUS       DACEJZ010000109         8490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEJZ010000109 DACEJZ010000000
VERSION     DACEJZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.112"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..173)
                     /locus_tag="IW656_002506"
     CDS             complement(<1..173)
                     /locus_tag="IW656_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0838188.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IW656_002507"
     CDS             complement(593..1714)
                     /locus_tag="IW656_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0838189.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IW656_002508"
     CDS             2042..3451
                     /locus_tag="IW656_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0838190.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IW656_002509"
     CDS             3448..4176
                     /locus_tag="IW656_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0838191.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IW656_002510"
     CDS             complement(4304..5215)
                     /locus_tag="IW656_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838192.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IW656_002511"
     CDS             complement(5232..6239)
                     /locus_tag="IW656_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0838193.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIVIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IW656_002512"
     CDS             complement(6236..8365)
                     /locus_tag="IW656_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0838194.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IW656_002513"
     CDS             complement(8370..8477)
                     /locus_tag="IW656_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838195.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2601 a         1726 c         1484 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 acgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DACEJZ010000110         8335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEJZ010000110 DACEJZ010000000
VERSION     DACEJZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.113"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            264..1457
                     /locus_tag="IW656_002514"
     CDS             264..1457
                     /locus_tag="IW656_002514"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0838196.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IW656_002515"
     CDS             1587..3059
                     /locus_tag="IW656_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0838197.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IW656_002516"
     CDS             3142..3930
                     /locus_tag="IW656_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0838198.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IW656_002517"
     CDS             complement(3997..4665)
                     /locus_tag="IW656_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0838199.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IW656_002518"
     CDS             4780..6492
                     /locus_tag="IW656_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0838200.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IW656_002519"
     CDS             6495..8267
                     /locus_tag="IW656_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0838201.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2551 a         1391 c         1828 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggca
//
LOCUS       DACEJZ010000111         8303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEJZ010000111 DACEJZ010000000
VERSION     DACEJZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.114"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            47..679
                     /locus_tag="IW656_002520"
     CDS             47..679
                     /locus_tag="IW656_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0838202.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="IW656_002521"
     CDS             complement(681..1586)
                     /locus_tag="IW656_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0838203.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2324)
                     /locus_tag="IW656_002522"
     CDS             complement(1671..2324)
                     /locus_tag="IW656_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0838204.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="IW656_002523"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="IW656_002523"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0838205.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="IW656_002524"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="IW656_002524"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0838206.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="IW656_002525"
     CDS             4573..5658
                     /locus_tag="IW656_002525"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0838207.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="IW656_002526"
     CDS             5655..6719
                     /locus_tag="IW656_002526"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0838208.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="IW656_002527"
     CDS             complement(6968..7771)
                     /locus_tag="IW656_002527"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0838209.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IW656_002528"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="IW656_002528"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8303)
                     /locus_tag="IW656_002529"
     CDS             complement(8078..>8303)
                     /locus_tag="IW656_002529"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ0838210.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2509 a         1741 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgcccac aaa
//
LOCUS       DACEJZ010000112         8251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEJZ010000112 DACEJZ010000000
VERSION     DACEJZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.115"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW656_002530"
     CDS             <1..197
                     /locus_tag="IW656_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838211.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW656_002531"
     CDS             314..811
                     /locus_tag="IW656_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0838212.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW656_002532"
     CDS             898..1290
                     /locus_tag="IW656_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838213.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IW656_002533"
     CDS             1274..3721
                     /locus_tag="IW656_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0838214.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IW656_002534"
     CDS             3724..5907
                     /locus_tag="IW656_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0838215.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IW656_002535"
     CDS             5904..6905
                     /locus_tag="IW656_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0838216.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IW656_002536"
     CDS             6920..7834
                     /locus_tag="IW656_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838217.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IW656_002537"
     CDS             8079..8195
                     /locus_tag="IW656_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0838218.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DACEJZ010000113         8066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEJZ010000113 DACEJZ010000000
VERSION     DACEJZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.116"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..882
                     /locus_tag="IW656_002538"
     CDS             <1..882
                     /locus_tag="IW656_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0838219.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            930..3059
                     /locus_tag="IW656_002539"
     CDS             930..3059
                     /locus_tag="IW656_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0838220.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3034..3723
                     /locus_tag="IW656_002540"
     CDS             3034..3723
                     /locus_tag="IW656_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838221.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4131..4817)
                     /locus_tag="IW656_002541"
     CDS             complement(4131..4817)
                     /locus_tag="IW656_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0838222.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4953..5147)
                     /locus_tag="IW656_002542"
     CDS             complement(4953..5147)
                     /locus_tag="IW656_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838223.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="IW656_002543"
     CDS             complement(5197..5637)
                     /locus_tag="IW656_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0838224.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="IW656_002544"
     CDS             complement(5641..6486)
                     /locus_tag="IW656_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0838225.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="IW656_002545"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="IW656_002545"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0838226.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2357 t
ORIGIN      
        1 atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct
       61 aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa
      121 agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt
      181 gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca
      241 aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca
      301 gttttagggg tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc
      361 tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt
      421 gcgaaattat ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc
      481 ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga
      541 gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta
      601 ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg
      661 ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg
      721 attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca
      781 aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg
      841 atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa
      901 aaacgattag ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg
      961 ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa
     1021 aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt
     1081 cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac
     1141 attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa
     1201 tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg
     1261 atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa
     1321 acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg
     1381 tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg
     1441 aagcagcatc tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg
     1501 gattgacaag aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag
     1561 atacggcaac ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga
     1621 ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa
     1681 ccatgctttc cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta
     1741 atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac
     1801 gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct tcattagatg
     1861 ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg
     1921 atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt acggagacag
     1981 aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt
     2041 acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca
     2101 atgttcttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa
     2161 aagccataca ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga
     2221 tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc
     2281 acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta
     2341 cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt
     2401 cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc
     2461 caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag
     2521 gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa
     2581 cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa
     2641 cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct
     2701 tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg
     2761 gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat
     2821 gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg
     2881 cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag
     2941 tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat
     3001 ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag
     3061 ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg attctaccag
     3121 tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa
     3181 caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc
     3241 gatgtaggag aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa
     3301 ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg
     3361 gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa
     3421 gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat
     3481 acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt
     3541 cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt
     3601 tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg
     3661 atcttagaag aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa
     3721 taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc
     3781 ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa
     3841 attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc
     3901 aaaagcttgc atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta
     3961 cttggctgtt cttattactt gatgcagggc agctttttca caacctctca ataaacggtg
     4021 tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt
     4081 tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg
     4141 ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc
     4201 tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca
     4261 atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa
     4321 atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc
     4381 caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DACEJZ010000114         7609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEJZ010000114 DACEJZ010000000
VERSION     DACEJZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.118"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(37..642)
                     /locus_tag="IW656_002546"
     CDS             complement(37..642)
                     /locus_tag="IW656_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0838227.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(838..1302)
                     /locus_tag="IW656_002547"
     CDS             complement(838..1302)
                     /locus_tag="IW656_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0838228.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(1324..1644)
                     /locus_tag="IW656_002548"
     CDS             complement(1324..1644)
                     /locus_tag="IW656_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0838229.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="IW656_002549"
     CDS             complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="IW656_002549"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0838230.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            3066..3416
                     /locus_tag="IW656_002550"
     CDS             3066..3416
                     /locus_tag="IW656_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0838231.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            3601..4554
                     /locus_tag="IW656_002551"
     CDS             3601..4554
                     /locus_tag="IW656_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0838232.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            4567..6354
                     /locus_tag="IW656_002552"
     CDS             4567..6354
                     /locus_tag="IW656_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838233.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            6341..7543
                     /locus_tag="IW656_002553"
     CDS             6341..7543
                     /locus_tag="IW656_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838234.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         2168 a         1446 c         1366 g         2629 t
ORIGIN      
        1 aaaaagaggt tgtgacaaag acttgtcaca acctctttat tttggaaata cttctccgac
       61 tacggcatcc tcaggcaata ctaagattcc ttttttttct ggagcatttg gcaaatgcaa
      121 ttcacgagca gaacaaatca ttccatagct ggctacccct cgcaaagttc ctggccagat
      181 catcattcca tctggcatca tggctcctgg ttttgcaacg acaacttttt gtccagcttc
      241 gatatttggt gcaccgcaaa caatttgcaa tactgtccca ttgtccactt ctgtctcagt
      301 aatcgataaa tggtcagagt caggatgcgg gacgcatgtt tttacatacc cgacaacgat
      361 cttcggttca tgatcagctt caagctgttc ttcgaatcct gcttctttaa tcaatcgatt
      421 caatttcgct aattcttcat ctgttaagct tacttggccg ttcccttcaa ttgttagatg
      481 atcggagata tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc
      541 aacattccat ttacgttcga cagtgttttc tgttccttta tcgtcggcta tgataaccat
      601 caaagtgtct ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc
      661 ctttgctgag atttatctta acaaaaaatt gcattcactg catgagattt ttttcatgct
      721 ttatttccga agaagcttct tctggaacac catttattcg taacaaggga ctgtgacaag
      781 acttttgtca cagtcccttt actgtttgat taaaaaggtt atacaagttc cacttgctta
      841 attactcggc tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat
      901 gacgtagctg gctacacttc ctgtcataaa gcgttcgact gcatttagtc cagactgccc
      961 aaccatgatt agatcgattg catatttttt tggcaatttg actgccatgg attcacgaac
     1021 agagccgtat gcaataattc cttctatatt tgtgtaatca acagattttc catattcttt
     1081 acattcttcg atcatttgct tggctgtttc agtttgctga tcgactagat tttgatcaat
     1141 aggagcatag cccataaaat tataaaattg ctgatcgatt acgtttgcta catatacttt
     1201 cccattatta cggcgagcca cttctaacgc ttttttgaaa gcagcaagtg cttgttcact
     1261 accgtcaata cctactaaaa tgttcttata ttcttgattc atcttgaccc ctcctaacag
     1321 atgtcagcct tttattacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc
     1381 tttattcact agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc
     1441 aaaaatgttc cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg
     1501 ccaatcagaa aagtcatttt cgatttcagg catttgcggt ttgataaaac ggcagtctcc
     1561 gcaccagccg gcagtaaaga aaaatacact ttttccagtt tctacgtagc ctgctaattc
     1621 ttctaaacta tttgggataa tcattctttt tcctctactt tctgtttatg tatttttcaa
     1681 ttaaattata cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat
     1741 ctgacaaaag gttgcttttg ccagatttta tagtttcact atcgcgttat ttcccgtata
     1801 cgatcgtgtt cactgttgat ttatctaaac ctttcaatgt ttgaatcaac atttctcgtg
     1861 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     1921 cgccaatcac tgtacttggg attccttcat tttgtgtatg agcagcacct gcatctgtac
     1981 cgccttttga aacaaagtat tgataaggga tgttgtttgt ttcagcgata tctagcaagt
     2041 actcacgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     2101 ctcctaaatg tccgaatgtg ccatttttcg tcactgtgtc atctgctgca gagcagtcta
     2161 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     2221 cttcttcttg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgtaacg
     2281 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccacgat ttgctgatga
     2341 tattcttccc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa
     2401 gaacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatctg
     2461 ttacttctac tgatttttgt ccgcttgttc ctcgtaataa atgtggggga acggaagaag
     2521 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgttga gcagaaacaa
     2581 cgtaagggtt ccatccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     2641 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacgaggt gcatccgctt
     2701 cttcatggtg gcgcagccca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     2761 gcggttccat atgactttcc atataggcgc gaacgtcttg ttcaaaaccg cttgttcctt
     2821 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     2881 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     2941 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     3001 aaaatatgag agtgtggtaa aataaacaca aagagtagca agtgagaaga aggaggtata
     3061 tccacatgta tgaagaaagc gaattaagct attttaaagg cgggttagcc gttgggatcg
     3121 gtctaggatt attgagtggg gtagcttcaa cgatttggta tcatcgcaaa aaagtgttat
     3181 cagcagacca agtgcttgaa aatatcaaaa catctttttt aaaagaaggc gcaatcgaag
     3241 gtgcttggat ctcttttgaa aaagaaccta ttcggaaatt cgccgttcgg tcgagtgctt
     3301 atcggggtgg gctttcccgg atagaagatg gcgaaactgt ttattatgaa tttttagcag
     3361 atgctcatac aggaaccgta ttagacattt caagaaaaaa gggcgcaata gattaatacg
     3421 tcaaaaaaat gaaataacga atacgcaccc actatctaaa agagaggtat cctatccttc
     3481 ttttatagtg ggtgattttt ataaaaatag ggctatttcc tcttggaata gctagtattt
     3541 gtagttatta tacggtattc ttgaaataag attagtttta ttatataagg agagtgacaa
     3601 atggaatcac gtgaaaaaat tgccgtgctc attccttgtt ataacgaaga ggcgacaatt
     3661 tcaactgtta ttgaagattt taaaagggaa ttaccagaag cagatattta tgtctatgat
     3721 aataattcga ccgatcgaac atacgaattg gccgttcaag gcggagcaat cgtaaagaaa
     3781 gaaccacgtc aaggaaaagg gaatgtaatc cgtcaaatgt tttttgatat tgatgccgat
     3841 tattatttga tggtagatgg tgatgatact tatcctgcag aagctgcaca tggtttgctt
     3901 gagaaattac gttctggcga agcagatatg gtcattggcg atcgtctatc aaatggaacc
     3961 tattttgatg aaaataagcg tcctttccat gattttggga ataatctggt taaaaataca
     4021 attacttact tatataaaac aaaaattcgc gatgtcatga ccggctatcg aggattcaat
     4081 cgaatttttg tcaaaagctt ccctattatg agttctggtt ttcaaatcga aacagaattg
     4141 acgattcatg ctttagataa aaaattcaag ttcgttgaga ttccaatcga ttatcgcgat
     4201 cgtcctgaag gtagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     4261 atgatcatca aaatgtgcaa agattataaa cctttgcttt tctttgggat ctggaccatc
     4321 ttcttctttt tatttggatt ggtcatgggg attcctgtga ttcgtgaata tatgctgaca
     4381 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagccctt
     4441 ctttcattag taacagggtt gattttggat acggtcgtca caaatgccaa gaaagaatat
     4501 gaattgaatt tataccatgt ctaccacgaa tatcaaaata aagaaatgaa gtaggaggcc
     4561 ttttttatgc ataagaaaaa ggagaatcta tttcctactt tttttgcaac tttatttgcg
     4621 tcaggttttg ctttttttac ttcttatggt ctaggaactt tgaatccaac agtaatcatt
     4681 ggtttagcca tggttttgct acttgctttt aagcaatatg gaatagagtt gttttcaata
     4741 aaaaacaaag ttgcagctat tgcccgattg tttttttctg cagcttttgc tcttacactt
     4801 tctattggca gacagataca atttcagtat gtctcagcaa actatacaaa gacaacgatt
     4861 gctgatggaa gtatttttta ctttcttgtt tatttctcca tttttgtgta ttctttttta
     4921 tttttacaat cgatttattt atcaatgcca aaactggaaa cattttttac tgctcaaaac
     4981 acacaggctt tctcttcaag aaaatatttt ttattcttta ctgtcttttt agttctgtgt
     5041 tggcttcctt atctgttggc attgtatcct ggagtggttt tgcctgattc gttaagttcg
     5101 gtcgcccaat ctatgggtga tgttcctata agcaatcatc atccagtctt attcacctta
     5161 ctggtcaagc tatttgttca tgatttacca actgatacga tcaatcacgg tgtattttta
     5221 ttttcgattt ttcaaacatt gataacagct tcagcgataa gttattctct ttgctggctg
     5281 ttaaaaagaa acatccattt ttttccggta ctcattagtt tatgctattt tgcatttgca
     5341 ccggtttttc caatctacgc cttgaacatg caaaaagata ctttgttctc tatcgcttgc
     5401 tttttatttt ccttagcggt ttttgacttt atttttcaac gagaaaagcc aacgattctc
     5461 aatcagattt acgtaatcat ccttgcttta ctgaccacct atctaagaaa taatgggatt
     5521 tatgtagtaa ccggcacagg tttgatttgc ggcttgatta ttttgaaaca aaaaaggtat
     5581 caagcattct ttcaagtggt gctgggttat acagtgattg ctggactact catccacccg
     5641 ttgatggctc gttatgctat tcctacagaa actgctgaac aattaggtgt tcctttgcag
     5701 caaatttcta gaactattgt tatggatggt gacttgtcta agaaagataa agcattcatg
     5761 aatcagctac tcccgataaa agattatgaa gcgtattctc ctagtcttgc agactcaatc
     5821 aagtggcata aaaattttaa caatgagttt ttgaatacac ataaaaaaga atttctatca
     5881 ctatgggcaa gaaatttacc aagtaatata aaaatctata cagatgctta tattttagaa
     5941 acgttcgggt tctgggtacc tggtacaaaa aatagttacg gctttttaga tactcgtgta
     6001 aacacgaata attatggtat gaagcaagta gatttgattg aacatcttac tggattgtca
     6061 ggaatgaaaa aaatcatcga tgctcgtgat ttctttggtt ccggcacatt actgtggtta
     6121 gctatcggca gtgcactttt agcaatcttt aagaaaaaat atcggtattt actccttttt
     6181 cttcccggta tgcttgtttt tgctactatt atgattgcta ctcctgtggc ttttagttta
     6241 cggtatgtgt ttgttttagc attaggtcta cctgtttata tcctctctcc ttttttacta
     6301 gataaaagag aaaaaatgaa aacagatata ggagatactc atgaaactat ttaaaaagat
     6361 attttttgct tttgttctct tgatcgctag ctattgcgta ctactgacaa ttactttttt
     6421 cattccgcag cactctattg aatcgaatgc agaaaaatct ctacagatgg ttgaaaaaga
     6481 aggaatgtat ccttctatca atcaaggaaa tatgctaggc actcggttag ataattttac
     6541 tgatcatttg atgatacgaa aaacaaaagc agatcctgaa ttgaactcct tggagaacgc
     6601 gatgtctatg gcggattatc cccgttattg gcatggttat cagctttttt tacgaccttt
     6661 actgctaggg atgagtttgg ggagtattcg aatgatttac gcagccattc tttttctgct
     6721 gattggattg acgagctact ttttaaccaa gtgtagcgac atctatttaa cgacagcctt
     6781 gttgatttcg cttgctatag gaaatgctgc aacctttttc ttcagtatgc aattttcaaa
     6841 tatttggatt ttgactttat tgtctgtatt actattttta acaaaaccac aactctccaa
     6901 caccaaccaa caattattct tgtatttctt tgtcattggt tctttagcta acttttttga
     6961 cttgttgaca acaccggtca tctcttgggg gatacctgtg atcattattt attatgtaac
     7021 aaataagtat ttaatggata aacgcagttc attgagcaaa caagtaggca gttttgtttt
     7081 taccggtatc ttttggggat taggctatgg tttgacatgg gtggctaaat ggatcctatc
     7141 atcgatcata ttgaacaaaa acattgtaaa agatgcaatc aaccaaatcc tctttcgaac
     7201 ggaaggaaac gacaaatacc cgttgcatcg attagagatg ctgaaaagca atattagatt
     7261 gatgtatcca aaagtagcaa tattattgct attgattact tgtcttgttt ttctctattt
     7321 tgcttatcgt aaacgaaata gctggcgtcc gttccaactt ctagttttgt ttgttttagc
     7381 tgctgtaacg ccgtatattt ggtacaatat tttagctaat cactcacaga ttcattattg
     7441 gtttacgtat cgtacgcaaa tcatcacaag ttttgctata cttagtgctt tcgccttttt
     7501 gatccctcct gagaaatcaa aagacgaact aaattacttt taagaatacc gatgtactaa
     7561 ttcattttta aacataaaaa ctccagctgt cagagatagc cggagtttt
//
LOCUS       DACEJZ010000115         7598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEJZ010000115 DACEJZ010000000
VERSION     DACEJZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.119"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..427)
                     /locus_tag="IW656_002554"
     CDS             complement(137..427)
                     /locus_tag="IW656_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838235.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IW656_002555"
     CDS             complement(545..805)
                     /locus_tag="IW656_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838236.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IW656_002556"
     CDS             complement(942..1355)
                     /locus_tag="IW656_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0838237.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IW656_002557"
     CDS             complement(1811..2296)
                     /locus_tag="IW656_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838238.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2680)
                     /locus_tag="IW656_002558"
     CDS             complement(2435..2680)
                     /locus_tag="IW656_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0838239.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IW656_002559"
     CDS             2873..3673
                     /locus_tag="IW656_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0838240.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IW656_002560"
     CDS             3692..5560
                     /locus_tag="IW656_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0838241.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IW656_002561"
     CDS             5553..6044
                     /locus_tag="IW656_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0838242.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IW656_002562"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IW656_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0838243.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="IW656_002563"
     CDS             6604..>7598
                     /locus_tag="IW656_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0838244.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1443 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga agctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatgcgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg ttcccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DACEJZ010000116         7553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEJZ010000116 DACEJZ010000000
VERSION     DACEJZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.120"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(571..1917)
                     /gene="gor"
                     /locus_tag="IW656_002564"
     CDS             complement(571..1917)
                     /gene="gor"
                     /locus_tag="IW656_002564"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0838245.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2051..3292)
                     /locus_tag="IW656_002565"
     CDS             complement(2051..3292)
                     /locus_tag="IW656_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0838246.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3310..4233)
                     /locus_tag="IW656_002566"
     CDS             complement(3310..4233)
                     /locus_tag="IW656_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0838247.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4454..6946)
                     /locus_tag="IW656_002567"
     CDS             complement(4454..6946)
                     /locus_tag="IW656_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0838248.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6953..7420)
                     /locus_tag="IW656_002568"
     CDS             complement(6953..7420)
                     /locus_tag="IW656_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0838249.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2079 a         1641 c         1213 g         2620 t
ORIGIN      
        1 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
       61 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      121 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
      181 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
      241 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
      301 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
      361 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
      421 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
      481 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
      541 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
      601 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
      661 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
      721 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
      781 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
      841 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
      901 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
      961 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     1021 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     1081 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     1141 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     1201 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     1261 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     1321 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     1381 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     1441 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     1501 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     1561 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     1621 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     1681 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     1741 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     1801 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     1861 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     1921 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     1981 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     2041 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     2101 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     2161 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     2221 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     2281 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     2341 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     2401 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     2461 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     2521 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     2581 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     2641 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     2701 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     2761 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     2821 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     2881 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     2941 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     3001 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     3061 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     3121 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     3181 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     3241 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     3301 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     3361 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     3421 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     3481 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     3541 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     3601 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     3661 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     3721 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     3781 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     3841 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     3901 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     3961 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     4021 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     4081 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     4141 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     4201 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     4261 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     4321 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     4381 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     4441 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     4501 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     4561 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     4621 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     4681 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     4741 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     4801 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     4861 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     4921 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     4981 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     5041 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     5101 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     5161 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     5221 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     5281 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     5341 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     5401 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     5461 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     5521 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     5581 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     5641 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     5701 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     5761 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     5821 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     5881 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     5941 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     6001 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     6061 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     6121 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     6181 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     6241 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     6301 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     6361 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     6421 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     6481 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     6541 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     6601 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     6661 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     6721 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     6781 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     6841 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     6901 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     6961 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     7021 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     7081 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     7141 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     7201 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     7261 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     7321 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     7381 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     7441 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     7501 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DACEJZ010000117         7531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEJZ010000117 DACEJZ010000000
VERSION     DACEJZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.121"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            199..282
                     /locus_tag="IW656_002569"
     CDS             199..282
                     /locus_tag="IW656_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0838250.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            407..1144
                     /gene="erm(B)"
                     /locus_tag="IW656_002570"
     CDS             407..1144
                     /gene="erm(B)"
                     /locus_tag="IW656_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0838251.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1314..1574
                     /locus_tag="IW656_002571"
                     /pseudo
     CDS             1314..1574
                     /locus_tag="IW656_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1737..2402
                     /locus_tag="IW656_002572"
                     /pseudo
     CDS             1737..2402
                     /locus_tag="IW656_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2438..2683
                     /locus_tag="IW656_002573"
     CDS             2438..2683
                     /locus_tag="IW656_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0838252.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2786..3655
                     /locus_tag="IW656_002574"
     CDS             2786..3655
                     /locus_tag="IW656_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0838253.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3636..4370
                     /locus_tag="IW656_002575"
     CDS             3636..4370
                     /locus_tag="IW656_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0838254.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4403..5315
                     /gene="ant(6)"
                     /locus_tag="IW656_002576"
                     /pseudo
     CDS             4403..5315
                     /gene="ant(6)"
                     /locus_tag="IW656_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5312..5854
                     /gene="sat4"
                     /locus_tag="IW656_002577"
     CDS             5312..5854
                     /gene="sat4"
                     /locus_tag="IW656_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0838255.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5947..6741
                     /locus_tag="IW656_002578"
                     /note="aph(3')-IIIa"
     CDS             5947..6741
                     /locus_tag="IW656_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0838256.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6849..7037)
                     /locus_tag="IW656_002579"
                     /pseudo
     CDS             complement(6849..7037)
                     /locus_tag="IW656_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7017..>7531
                     /locus_tag="IW656_002580"
     CDS             7017..>7531
                     /locus_tag="IW656_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0838257.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2630 a         1148 c         1568 g         2185 t
ORIGIN      
        1 acggctacaa taatcacatc ctgctgaaat tgctttcctt taaaatgatt catcgtcatt
       61 cctcctgcta tctttttcta ttattctacc ttatttgata gtagatttaa aactttgcaa
      121 cagaaccatt ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt
      181 aagaaggagg gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact
      241 gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc
      301 taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt
      361 aacttctgta tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat
      421 aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt
      481 gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac
      541 gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa
      601 cttatcgtca gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct
      661 acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt
      721 aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat
      781 tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt
      841 gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa
      901 accaaaagta aacagtgtct taataaaact tacccgccat accacagatg ttccagataa
      961 atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact
     1021 gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac
     1081 cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa
     1141 ataattctat gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag
     1201 ataaattatt agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta
     1261 cggggaattt gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag
     1321 gaaaatgtcc taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa
     1381 attatcctga atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa
     1441 cgaatgtaaa agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag
     1501 agaaaaagcc ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct
     1561 ctttttcaaa ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc
     1621 tttccattta gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat
     1681 aaagcacact ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt
     1741 atcaaaatta taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag
     1801 aaaatgattt tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc
     1861 ttccttatta tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta
     1921 ttctttgtgc ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag
     1981 atagtattcg gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc
     2041 gttttcgcgt tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg
     2101 agctactggt tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa
     2161 ttgaagcgga tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg
     2221 atagccttga tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac
     2281 ctgagttaat agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc
     2341 aatctttgga agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag
     2401 aagggggctg acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt
     2461 ggaagaagct attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga
     2521 tttattcaag ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac
     2581 gttattttta aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc
     2641 atctaatcag cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt
     2701 aaaaaaatta taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag
     2761 tgaatcgata aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa
     2821 attatcctct ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac
     2881 ccatacagag gattcggata tagatatcgg catctattac aattcagaat catttgacat
     2941 aaatactatt aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt
     3001 acctcccgga gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta
     3061 tcatgtggat ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga
     3121 gcacggaatt gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat
     3181 gtatcgagga gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga
     3241 attaaaaaaa caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt
     3301 tatgtttgaa gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa
     3361 atattatatt gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc
     3421 atgtaataat gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga
     3481 acataaacct gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc
     3541 actttttgaa tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc
     3601 ggagataaat aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata
     3661 atatattttt tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg
     3721 gagaatggga gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt
     3781 taggatgcgg ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag
     3841 taggtgttga tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac
     3901 agattgaata tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg
     3961 taatactaag ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga
     4021 tatataggat gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta
     4081 ctgctcatgg aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg
     4141 tggacaatta ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa
     4201 aatatcatag aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa
     4261 atcagattgt ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg
     4321 aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa
     4381 aaaataaacg aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc
     4441 tttagcagaa caggatgaac gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa
     4501 atattaatat acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata
     4561 tagaaccgtt tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc
     4621 aaaagccgga ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc
     4681 tatttgatga ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt
     4741 acctaaaggg cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg
     4801 acatagttcc gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg
     4861 attgctgcaa tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg
     4921 aaattttatt tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga
     4981 tatcatggaa ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt
     5041 ttattgaaag gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg
     5101 attcctatga aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat
     5161 ccggtgaggt ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa
     5221 aatataccag ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat
     5281 atgccgctga tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct
     5341 gaaagatatc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     5401 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     5461 ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac
     5521 tgataagata atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg
     5581 aaaaaattgg aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg
     5641 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     5701 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     5761 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     5821 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     5881 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     5941 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     6001 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     6061 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     6121 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     6181 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     6241 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     6301 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     6361 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     6421 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     6481 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     6541 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     6601 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     6661 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     6721 ttactggatg aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc
     6781 tttttagaca tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta
     6841 tatcttttct aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg
     6901 aagaggtttg tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct
     6961 agcttaagaa cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga
     7021 acaggattaa cagagtaacc tctattctta ttcagctgca atcaaaaaaa ataattcctg
     7081 ccaaagaaat tgcacagcga tttaatataa gcttaaggac agtttacaga gatatccgga
     7141 cacttgaaga agccggaata ccaattggat ctgaggccgg aaaaggatat tttcttgtag
     7201 agggcttcct acttccgccg gtaatgttta cagcggcgga agtgggtgca ttgattacag
     7261 cagggaaatt tttaaattgc catggagatg aatcatttat aaaggatttt gattcagcta
     7321 tgtataaaat caaatctatt ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga
     7381 acagtattaa cgtgtacagc acatctggac agaaaaatac attggcggat aacgttattg
     7441 cagcaattca gactgcaatc tgcaataaaa gggtaatatc aattcaatat cctgcatcag
     7501 gagggcaaga accggaaagc agaatgatag a
//
LOCUS       DACEJZ010000118         7352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEJZ010000118 DACEJZ010000000
VERSION     DACEJZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.123"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..354)
                     /locus_tag="IW656_002581"
     CDS             complement(58..354)
                     /locus_tag="IW656_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0838258.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IW656_002582"
     CDS             709..1065
                     /locus_tag="IW656_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0838259.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IW656_002583"
     CDS             1075..1974
                     /locus_tag="IW656_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0838260.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IW656_002584"
     CDS             2491..3222
                     /locus_tag="IW656_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0838261.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3367..5394
                     /locus_tag="IW656_002585"
     CDS             3367..5394
                     /locus_tag="IW656_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0838262.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5502..6599
                     /locus_tag="IW656_002586"
     CDS             5502..6599
                     /locus_tag="IW656_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0838263.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6906..>7352
                     /locus_tag="IW656_002587"
     CDS             6906..>7352
                     /locus_tag="IW656_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0838264.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         2555 a         1181 c         1463 g         2153 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
     3361 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
     3421 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
     3481 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
     3541 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
     3601 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
     3661 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
     3721 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
     3781 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
     3841 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
     3901 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
     3961 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
     4021 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
     4081 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
     4141 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
     4201 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt
     4261 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
     4321 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
     4381 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
     4441 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
     4501 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
     4561 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
     4621 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
     4681 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
     4741 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
     4801 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
     4861 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
     4921 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
     4981 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
     5041 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
     5101 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
     5161 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
     5221 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
     5281 ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc
     5341 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
     5401 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
     5461 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
     5521 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
     5581 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
     5641 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
     5701 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
     5761 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
     5821 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
     5881 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
     5941 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
     6001 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
     6061 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
     6121 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
     6181 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
     6241 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
     6301 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
     6361 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
     6421 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
     6481 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
     6541 ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag
     6601 gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct ttttataaca
     6661 atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat aattctaata
     6721 acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa aaaaaagaca
     6781 ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat ccaggaaatc
     6841 agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga aagagctgat
     6901 gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat tcattactta
     6961 aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa gaagagttgt
     7021 cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat ttacaaaagt
     7081 tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt ctggaaatta
     7141 atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct ttatctatga
     7201 aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt gctaaacagc
     7261 atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga aatctaaaaa
     7321 aatatcaaat tttttttgaa aagagaaagt tg
//
LOCUS       DACEJZ010000119         7153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEJZ010000119 DACEJZ010000000
VERSION     DACEJZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.124"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1368)
                     /locus_tag="IW656_002588"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="IW656_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ0838265.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="IW656_002589"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="IW656_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0838266.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="IW656_002590"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="IW656_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="IW656_002591"
     CDS             complement(2652..2882)
                     /locus_tag="IW656_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0838267.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="IW656_002592"
     CDS             complement(3112..3930)
                     /locus_tag="IW656_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0838268.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="IW656_002593"
     CDS             4091..4780
                     /locus_tag="IW656_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0838269.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="IW656_002594"
     CDS             4794..6296
                     /locus_tag="IW656_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0838270.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="IW656_002595"
     CDS             6309..6785
                     /locus_tag="IW656_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0838271.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="IW656_002596"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="IW656_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2316 a         1131 c         1295 g         2411 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DACEJZ010000120         6625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEJZ010000120 DACEJZ010000000
VERSION     DACEJZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.125"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW656_002597"
     CDS             <1..1023
                     /locus_tag="IW656_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0838272.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IW656_002598"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IW656_002598"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0838273.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IW656_002599"
     CDS             3389..3985
                     /locus_tag="IW656_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0838274.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IW656_002600"
     CDS             3998..4897
                     /locus_tag="IW656_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0838275.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IW656_002601"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IW656_002601"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0838276.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IW656_002602"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IW656_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0838277.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IW656_002603"
     CDS             5430..5663
                     /locus_tag="IW656_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838278.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IW656_002604"
     CDS             complement(5822..5944)
                     /locus_tag="IW656_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0838279.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IW656_002605"
     CDS             6134..6358
                     /locus_tag="IW656_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838280.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
BASE COUNT         2371 a         1166 c         1399 g         1689 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttg
//
LOCUS       DACEJZ010000121         6467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEJZ010000121 DACEJZ010000000
VERSION     DACEJZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.126"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..564)
                     /locus_tag="IW656_002606"
     CDS             complement(109..564)
                     /locus_tag="IW656_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838281.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IW656_002607"
     CDS             complement(718..1926)
                     /locus_tag="IW656_002607"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0838282.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IW656_002608"
     CDS             complement(2038..2370)
                     /locus_tag="IW656_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0838283.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IW656_002609"
     CDS             2518..3393
                     /locus_tag="IW656_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0838284.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IW656_002610"
     CDS             complement(3475..4056)
                     /locus_tag="IW656_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0838285.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IW656_002611"
     CDS             complement(4062..5321)
                     /locus_tag="IW656_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0838286.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IW656_002612"
     CDS             complement(5323..6369)
                     /locus_tag="IW656_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838287.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1804 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaa
//
LOCUS       DACEJZ010000122         6413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEJZ010000122 DACEJZ010000000
VERSION     DACEJZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.127"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            78..1382
                     /locus_tag="IW656_002613"
     CDS             78..1382
                     /locus_tag="IW656_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0838288.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1474..1839)
                     /locus_tag="IW656_002614"
     CDS             complement(1474..1839)
                     /locus_tag="IW656_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0838289.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1836..2201)
                     /locus_tag="IW656_002615"
     CDS             complement(1836..2201)
                     /locus_tag="IW656_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0838290.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2393..2641)
                     /locus_tag="IW656_002616"
     CDS             complement(2393..2641)
                     /locus_tag="IW656_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838291.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2640..2768
                     /locus_tag="IW656_002617"
     CDS             2640..2768
                     /locus_tag="IW656_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0838292.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2825..4519
                     /locus_tag="IW656_002618"
     CDS             2825..4519
                     /locus_tag="IW656_002618"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0838293.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4601..4792
                     /locus_tag="IW656_002619"
     CDS             4601..4792
                     /locus_tag="IW656_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838294.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5170..6303
                     /locus_tag="IW656_002620"
     CDS             5170..6303
                     /locus_tag="IW656_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0838295.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         2122 a         1222 c         1169 g         1900 t
ORIGIN      
        1 tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc aatctcctgc
       61 atgtttgcaa aaccactttg ctcaatgata tcaataacat aaaaaaagaa tttgaagaac
      121 agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca gaacatatac
      181 ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa acttgtctgc
      241 tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa ttcatctctg
      301 tcagcaaagc ttattcatta aaaaaacaag tattagctta ttttaatgac tgcgggatcg
      361 aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg cttcttaatg
      421 tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt tttgaacgtg
      481 ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat aaagaaaata
      541 aagaaatctt attgattggt tttgctatca gtttcctacg ccagcaagtg tgtgctgtga
      601 caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat
      661 cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc gcctttattt
      721 tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt gcggaagact
      781 ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa ctagttgcta
      841 cctttgaatc tcacttcaat caagaattat tcggaaacca accattcgaa cgtgcgctga
      901 ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa aaattttatc
      961 ttctaaatga ggaacagaca aatttatata aagaagtcca gacaattttt tccagctggt
     1021 ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct tttgtgatcg
     1081 aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc gtcacaaatt
     1141 cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact ttcgattttc
     1201 aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg cagcagtcct
     1261 caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa tatgaaaata
     1321 ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg caaaacaaat
     1381 aagggactgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc ccatttctgt
     1441 aacaatctct tgatacacat caggctcaca acctcactta ttttggacat ctacgggtcc
     1501 gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg ccgctagttg
     1561 gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg aaacaagaat
     1621 ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt tctcgctgac
     1681 tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct tgattgatag
     1741 acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta caagtttttc
     1801 aaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt
     1861 ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg aatgataata
     1921 ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc gatgatatca
     1981 gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt tcttgtatct
     2041 gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc aaaaagtttg
     2101 aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt taaaatagca
     2161 atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac ctactttctt
     2221 ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt
     2281 tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccctt tttctccctc
     2341 catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag gcttagtccg
     2401 tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa
     2461 tagaaccata cacctcctca aatcttttct tcttacctac cctattttta tttacaggaa
     2521 aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc
     2581 attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag gcgtttttca
     2641 tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat
     2701 atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat gaattcgaat
     2761 caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca ttggaggaat
     2821 cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata tggcagcggc
     2881 tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt attcattgga
     2941 tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat attggaataa
     3001 aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca tgcttaatta
     3061 ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta ctgagccagg
     3121 gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct ttgacaaagc
     3181 acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat tcaataataa
     3241 agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag aaaaactaga
     3301 caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa caaacttctg
     3361 gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag aattgcgtcg
     3421 ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg
     3481 aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact tgaaagaaca
     3541 aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag aaacaccgat
     3601 gcaagatgaa atcactgtga ctggtttgaa aatgaccaat acactgactc aagaagaaga
     3661 acacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca ttacagattc
     3721 agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg gtgcagcttc
     3781 tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg acaaattctt
     3841 tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa accatttgtt
     3901 gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga attcatttat
     3961 ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta tcgtcgtgca
     4021 tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg gttatttcct
     4081 ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga atggaaaaga
     4141 aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag tgaatattcg
     4201 tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct atatgcctta
     4261 tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca
     4321 ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga tgatctttac
     4381 tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg gtatccctat
     4441 gtcacgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag ctcgtgccgc
     4501 aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc aagtaataag
     4561 aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat taccgcagat
     4621 gcaagctttt gaacaccgtt taccaagagg ttgtgaaaaa gctgccctgc atcaagtaat
     4681 aagagcagcc aagctaaaac agcttcttat gtttttgact tggttggact tattaccgca
     4741 gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaagtcgttt agtttcgagt
     4801 atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg acttaatacc
     4861 gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct ttttgaaaac
     4921 aaccattttt tcaacgttta ctctcttgcc aatccaacat catctaacag atacaactat
     4981 ttcaatgatt accattacac taaaaaatcg gaaactcacc tctccgaaca caaatacata
     5041 agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc
     5101 ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa tctgaaagga
     5161 gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat cagaaacgta
     5221 gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc aacagccatt
     5281 tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt
     5341 gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca aacattgaaa
     5401 aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca tttctgcctg
     5461 aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg cgggcttttc
     5521 ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct aagagttcct
     5581 tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga aaaagtttta
     5641 tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa
     5701 ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca aaaattcgct
     5761 gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg tctagataaa
     5821 gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca agctggaaca
     5881 gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt aggaatggac
     5941 ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaaa cggcacaatc
     6001 tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca agtaactggt
     6061 gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg ggatatcgct
     6121 gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacga aatgattgca
     6181 gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc agaagcagca
     6241 caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt tttgaaacct
     6301 tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg aaatacccat
     6361 gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt ccg
//
LOCUS       DACEJZ010000123         6401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEJZ010000123 DACEJZ010000000
VERSION     DACEJZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.128"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..579
                     /locus_tag="IW656_002621"
     CDS             127..579
                     /locus_tag="IW656_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838296.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IW656_002622"
     CDS             593..1282
                     /locus_tag="IW656_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838297.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IW656_002623"
     CDS             1407..3758
                     /locus_tag="IW656_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0838298.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IW656_002624"
     CDS             3838..4233
                     /locus_tag="IW656_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838299.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IW656_002625"
     CDS             4558..5175
                     /locus_tag="IW656_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838300.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IW656_002626"
     CDS             5216..5515
                     /locus_tag="IW656_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838301.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IW656_002627"
     CDS             5549..5716
                     /locus_tag="IW656_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0838302.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IW656_002628"
     CDS             complement(5761..6324)
                     /locus_tag="IW656_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838303.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2193 a          898 c         1267 g         2043 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgaag ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag c
//
LOCUS       DACEJZ010000124         6345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEJZ010000124 DACEJZ010000000
VERSION     DACEJZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.129"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1014
                     /locus_tag="IW656_002629"
     CDS             <1..1014
                     /locus_tag="IW656_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0838304.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IW656_002630"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IW656_002630"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0838305.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IW656_002631"
     CDS             3470..4063
                     /locus_tag="IW656_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0838306.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IW656_002632"
     CDS             4076..4975
                     /locus_tag="IW656_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0838307.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IW656_002633"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IW656_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0838308.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IW656_002634"
     CDS             5817..6077
                     /locus_tag="IW656_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838309.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
BASE COUNT         2339 a         1094 c         1305 g         1607 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttg
//
LOCUS       DACEJZ010000125         6340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEJZ010000125 DACEJZ010000000
VERSION     DACEJZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.130"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            157..1131
                     /locus_tag="IW656_002635"
     CDS             157..1131
                     /locus_tag="IW656_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0838310.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1113..1574
                     /locus_tag="IW656_002636"
     CDS             1113..1574
                     /locus_tag="IW656_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0838311.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1624..2088
                     /locus_tag="IW656_002637"
     CDS             1624..2088
                     /locus_tag="IW656_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0838312.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2102..2851
                     /locus_tag="IW656_002638"
     CDS             2102..2851
                     /locus_tag="IW656_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0838313.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2851..3681
                     /locus_tag="IW656_002639"
     CDS             2851..3681
                     /locus_tag="IW656_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0838314.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3681..4328
                     /locus_tag="IW656_002640"
     CDS             3681..4328
                     /locus_tag="IW656_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0838315.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4426..5202)
                     /locus_tag="IW656_002641"
     CDS             complement(4426..5202)
                     /locus_tag="IW656_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0838316.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5284..5427
                     /locus_tag="IW656_002642"
                     /pseudo
     CDS             5284..5427
                     /locus_tag="IW656_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5544..6340)
                     /locus_tag="IW656_002643"
                     /pseudo
     CDS             complement(5544..6340)
                     /locus_tag="IW656_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         2121 a          894 c         1138 g         2187 t
ORIGIN      
        1 aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa aaaatttttt
       61 tgaaaaatag tattgcaaaa taaagcaatt tcatagataa taggtatcgt aaagttatat
      121 aactttataa tacctattag agtgagggat aaagaaatga gacaactaca caaagaacag
      181 attgaaacag caatggcagc gttaaaggaa aagacaattg atcatgtata ttttacagca
      241 tgtgggggtt cacaagccgt tttaatggct ggtcaatatc tatttgataa agaattaact
      301 gtaccttgtc atgtttatac tgctaacgaa tttaactatg ctataccaaa aggattatct
      361 gaaaattctt tagttatcac ttgttcccat tctggcacaa caccagaaac tgtagaagca
      421 acaaaaaaag caagagaatt tggagcaatg actatttgtt tatctaatga agaaggatct
      481 cctctatggg aagcagctga atatcctgtt cactatgatt ggggtaaaga tgttgatgct
      541 agtgatttaa ataaaggaat tctttatggt ttattattta atattttact tgttttagat
      601 ggctctaaaa aatgggaaac ttgcttgaat gaatttgata agttaaatga tcttgttatt
      661 gctgaaaaga gcaaatttga aataactgct aaagaatggg gtaaaaacca aaaacgagat
      721 aaaattattt acacaattgg tagcggtatt aattacggag aaatttactc cacagcagct
      781 tgctggttca tggaaatgca atggattaat tctaatgcta ttcattctgg ggaatttttc
      841 catggtcctt ttgaaatcac tgattatgat gtaccattca tcttagtgaa aagtattgga
      901 gatacaagat ttttagatca acgggtagaa gacttcacta aaaaattcag taataaatta
      961 atggttttag accaagctga ttttgactta gaatctatag atagtgtagc gaaagaatat
     1021 attgcagctg tacttagtgg cgttgttatt cgccaaatgg tagaagcgat tgcttttgaa
     1081 agaggccatt ccttagatgt tagacgctat atgtggcaaa tgaaatatta attaatagga
     1141 ggttgcaaaa gatggcaaat caattatttc acattgtgat tacaacacat tctacccttt
     1201 gtgagggtta tttaaaggca acaaaattaa ttttaatgaa tgacgccaca ggtattagtg
     1261 ctgttccatt taaagaaaat actagtccag atgattttga ggagagtatg aaagaaataa
     1321 ttagtcagca tcatttgcaa cctctaatta ttttaacgga tttaattgga ggaacaccta
     1381 ataatgtttc tattaaacag ttgaacaaca atagaattca aattattgct gggattaatt
     1441 tacctctttt actggagtta ttaatggccc agtcagctgg taaatctata gaagaaatag
     1501 attttaagga attactgaat aactctcaaa aaagtatggt tcatattaat tcgcttttaa
     1561 aagatgtcag ttaaggtggc aatttctatt ctataaattt taaaaattga aggaggaaat
     1621 agaatgatta aattagtgcg tgtagaccac agattacttc atggacaagt aatattttct
     1681 tggactaaac agtatgatat taaccatatt attgtggctg ataacaaagt tccaggagat
     1741 ccaatgtctg tgatggcttt gacaattgct aaacctgctg attgcaaatt agacattgtt
     1801 aaaattagtg atgttaaagg gattatggaa acatccccaa atgataatta catgatttta
     1861 attaaaggac ctaaggaagc ttttgaatta gttcaaaatt taccagaaat taaagaaatt
     1921 aattttggcg gtgtgtctaa gaaaagcgag tccaaacaat atggaaaggc agtcttttta
     1981 actcctgaag aattagaagc cacaaaaaaa attcttgatc aagaagtgaa aatatttgtg
     2041 caacaagtcc catcttcaag tattgaaaat gtagatttta gtaagtaagg ggggaaaata
     2101 aatgttatta aaagccattt tattaggttt agttggtgtg gtagctgtta ttgactccag
     2161 gttaattggt cgacaaaata ttggtagacc actaattttg agtacacttg ccggacttgt
     2221 gctaggtgac ttgcatacag ggattgttct tggggcatct ttagaattaa tttctatggg
     2281 attcgtctca attggggcgg ctggacctcc taatatgcaa cttggtagta ttattgccac
     2341 tgcttttgca attttaacta atagtagtac agaaacagcc ttaacaatag ctatcccagt
     2401 ggctgtagct ggtgaattta tgagtattat catgcgcatg ataattgctc aatttggaca
     2461 tgctgcagat aaagccattg aaaataataa atttagaaaa gctcaaatta ttcatattta
     2521 ttggtcattt atttttaatg cttttgttta ctttattcca atctttttaa cagtatattt
     2581 tggtgctgac gttgtggcag atttagtaga taaaattcca gaaatgatta cagacgcctt
     2641 gactgtctct ggaaatatgc tttccgcatt agggtttgca atgctattaa gcacaatgtt
     2701 gagtaaaaaa ttatatcctt actttatttt tggattcttt attgttgcct attcaggtct
     2761 ttcactaatt ggcgtgacaa tttttgcagc tctgattgcc tttgtaatgg atcaagttaa
     2821 atatggaaaa agagaagagg cgcatggtta atggaaaata gtacagaaaa aaaacgttta
     2881 atcacaaaaa aagatcttca taagatgtgt cttcgttcat ggacaatcat gtgttcgtgg
     2941 aattatgaaa gacaaatgca tatgggattt atgtatggga tggcgccaat tttaaataaa
     3001 gtttataaaa atgatgaaga gaaaaaaaga gaagcttata ctcgtcatat ggcatttttt
     3061 aactgtacgc ctcaaatgac tccatttatt atgggacttt gtgcttctat ggaagaacaa
     3121 aatgctcaag cagaagaagg aaactttaat actgaatcta tctccatgat taaaaccagt
     3181 ttaatgggtc cttttgctgg aattggtgat tcatttttcc aaggtacttt aagaattatt
     3241 acatttggga ttggattgtc ttttgctaag caaggtagta ttttaggacc gattttagct
     3301 gttggccttt ttgctatacc ttctatttta gctgtttttt acggcggtaa attaggatat
     3361 gagtctggca ataaatacct tactaaattg tatcaagaag gtcttatgga tcgagtaatg
     3421 caatttgctt ctatcattgg tttggctgta gtaggtggaa tggtggctag catggtgcca
     3481 attgtcaccc ctttaaaatt cactacaggg ggaacagaac ttgttttaca agacttactt
     3541 gattccatta ttcctggatt attaccactt gtatgtactt tgggaatata taaattgatt
     3601 aagaaaaatg ttagtacaaa tatgctctta ttaggttgta ttttggcagg gtttgtttta
     3661 agtataatag gtatcttata atgattaagg ctctaatttt tgatatggac ggagttttaa
     3721 ttgatagcga gattatgtat caaaagtggg tatttaattt tttaaaacaa gaaaatttaa
     3781 gctatccttt gtattcttat caaaaaaaga ttggaactac agcaagtatt tttgatgata
     3841 tagaaaagta caatgaggga tgtaacagtt tagaattgaa gggaaaattt actaaatatt
     3901 ggaaatcgca aaagattaat tacttggata tttttcctaa tatactaatt gaacagcttg
     3961 aatggttaaa aaagaaaaaa ataaaaatgg caattgcttc ttccagtcca atggatacta
     4021 taaatgaagt tgttaccact tgtaaaatag agtcatattt tgattgttta attagcggaa
     4081 gagatttgcc agaaagtaaa cctaatccta ctatattcct taaagcttca gaacagctca
     4141 gaattccagt tgaagaatgt ctggtgatag aagattctta taatggtata aaagctggta
     4201 aaagagcaaa tatgaaggtt cttgcaatta aagataaacg tttttctcaa gatgtaagtc
     4261 tagcagatga tgttatttac gatattaaat atctgcgaca agaagtacag gtgaaatttg
     4321 gaatataaat aagttttttg atgtatttag agaatattag taaaaataaa aatcatgccc
     4381 aaatctaatc aaaattgatt gctttgggta tgatttcttt taatatcaat ctattacttt
     4441 aggatctgaa tcatttttat gcaccgtatc taaagtgata ttttctgtga cttcaataac
     4501 ataactaatt ctatcacaac gaactgtgga ttgagaataa tgaaatggtt gattattttt
     4561 atctttgcca accttttcta aaataaatag cggatctcct actatacatt gcaataagtc
     4621 ggaaagctca ggtgttgcag ataaaccgtt tatttccata atagagctcg tagctttgac
     4681 atcatagtca tcttctagaa gtttatataa tgaagtatat tggctacctt tttcaaataa
     4741 agttggaaat ttttcttctg atgcgaaaaa attatcaatt cccatagggg caccgtcttc
     4801 ccacattaaa cgtttaatat tgtaaactct atcagttaca ttgatttgta atttattagc
     4861 taaaaactca tcagccttta taattttaga atgtaaaaca gtatatctaa ttttatgtcc
     4921 tcgggtagaa agagaatcgc taaatccatg aactccagct aattttaaac tttctttttt
     4981 ttgagttaca aaggtccctt tcccccttaa tcgttcaact gttccacttt ctgttaaaag
     5041 tttaaaagct tttcgaatgg taatcttact tactccataa attttacata attcttcttc
     5101 tgtaggtaat tgctctcctg gaagatattt tttttcacga atatcgttac gtatagaagc
     5161 ttctacttgt agatataatg gtctattatc ttgaaaattc atagtacact ccctaaaaag
     5221 ttatacttca ttatatcaca atttattaaa ataaaagatt aagatataaa tataaatgaa
     5281 ctagtctttg actaaccaat gcccgctttt gaaagaaaac aaattcattc tgttttaagt
     5341 aaaaatcctt atgttaccac tcattctgaa ggggatgagc cttatcgcta cttggttgtg
     5401 gaaccaagtg ataagacaat tttataaatt caatattaat taaaataaaa acaaacaaag
     5461 tagattattt tactttgttt gtttttttat tgggagcgtc aataattttg tgtaaataac
     5521 tcgtccttct gcaaaataat gggttactca gtaaacattg aagctaatgt atccgtcact
     5581 tgttggaagc ctttatggct tctatttaag aatttttgat tgtatgtatc aaagatggat
     5641 actaggaaac gctctagtga ttcttcattt tgaaactgct cttttctgcg gctgtatttc
     5701 ttgatttgtt tcttgaaaga ttcgattaaa ttagttgagt aaatgcttcg gcggatacca
     5761 ggcggaaact cataaaaagt caataaatct tgatttttta tcagtgactg cgtcacttta
     5821 ggataagttt tttgctactt ctcaatcata ctccctaaaa aggtatttgc ctcttccttt
     5881 gagctagctt gataaacagc cttaaaatca tcatagattt cttttcgatc tttgacacgt
     5941 actttatgag caatattacg ggaaacgtga acacagcaat gttggtattt agctttagga
     6001 taaatctggt gaatcgtatc tttcatgcct tttaagccat cggtaataaa cagtaagaca
     6061 tcatgaaccc ctctggagta aatgtcctgt aacaactcat tccaaacgta ggtcgattca
     6121 gtcggagcaa tcgcataact tagtacttct ttagtgccgt cttctcgtat accaatggca
     6181 atatagattg cttctttgga gacggtttgc cgttttaaag gaatataagt agcatccatg
     6241 aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc
     6301 agcactttag tcatgttgga catggtttgg ggcgtgtagt
//
LOCUS       DACEJZ010000126         6253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEJZ010000126 DACEJZ010000000
VERSION     DACEJZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.131"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            25..1857
                     /gene="tet(M)"
                     /locus_tag="IW656_002644"
     CDS             25..1857
                     /gene="tet(M)"
                     /locus_tag="IW656_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0838317.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     MSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYM
                     ERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVME
                     GIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE
                     PYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTF
                     FTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1955..3328
                     /gene="tet(L)"
                     /locus_tag="IW656_002645"
     CDS             1955..3328
                     /gene="tet(L)"
                     /locus_tag="IW656_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0838318.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3892..5154
                     /locus_tag="IW656_002646"
     CDS             3892..5154
                     /locus_tag="IW656_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0838319.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5399..>6253
                     /locus_tag="IW656_002647"
     CDS             5399..>6253
                     /locus_tag="IW656_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0838320.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKK"
BASE COUNT         2163 a          892 c         1290 g         1908 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta tgtggattct
     1081 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
     1141 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
     1201 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
     1261 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
     1321 agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata
     1381 cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa aatctgtttt
     1441 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
     1501 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     1561 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     1621 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     1681 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     1741 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     1801 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     1861 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
     1921 agatttgaat taaagtgtaa aggaggaatt aattatgaag tgtaatgaat gtaacagggt
     1981 taaatacaac caaattttaa tttggctttg cgttttatct ttttttagcg tattaaatga
     2041 aatggttttg aacgtctcat tacctgatat tgcaaatgat tttaataaac cacctgcgag
     2101 tacaaactgg gtgaacacag cctttatgtt aaccttttcc attggaacag ctgtatatgg
     2161 aaagctatct gatcaattag gcatcaaaag gttactccta tttggaatta taataaattg
     2221 tttcgggtcg gtaattgggt ttgttggcca ttctttcttt tccttactta ttatggctcg
     2281 ttttattcaa ggggctggtg cagctgcatt tccagcactc gtaatggttg tagttgcgcg
     2341 ctatattcca aaggaaaata ggggtaaagc atttggtctt attggatcga tagtagccat
     2401 gggagaagga gtcggtccag cgattggtgg aatgatagcc cattatattc attggtccta
     2461 tcttctactc attcctatga taacaattat cactgttccg tttcttatga aattattaaa
     2521 gaaagaagta aggataaaag gtcattttga tatcaaagga attatactaa tgtctgtagg
     2581 cattgtattt tttatgttgt ttacaacatc atatagcatt tcttttctta tcgttagcgt
     2641 gctgtcattc ctgatatttg taaaacatat caggaaagta acagatcctt ttgttgatcc
     2701 cggattaggg aaaaatatac cttttatgat tggagttctt tgtgggggaa ttatatttgg
     2761 aacagtagca gggtttgtct ctatggttcc ttatatgatg aaagatgttc accagctaag
     2821 tactgccgaa atcggaagtg taattatttt ccctggaaca atgagtgtca ttattttcgg
     2881 ctacattggt gggatacttg ttgatagaag aggtccttta tacgtgttaa acatcggagt
     2941 tacatttctt tctgttagct ttttaactgc ttcctttctt ttagaaacaa catcatggtt
     3001 catgacaatt ataatcgtat ttgttttagg tgggctttcg ttcaccaaaa cagttatatc
     3061 aacaattgtt tcaagtagct tgaaacagca ggaagctggt gctggaatga gtttgcttaa
     3121 ctttaccagc tttttatcag agggaacagg tattgcaatt gtaggtggtt tattatccat
     3181 acccttactt gatcaaaggt tgttacctat ggaagttgat cagtcaactt atctgtatag
     3241 taatttgtta ttactttttt caggaatcat tgtcattagt tggctggtta ccttgaatgt
     3301 atataaacat tctcaaaggg atttctaaat cgttaaggga tcaactttgg gagagagttc
     3361 aaaattgatc ctttttttat aacaggaatt caaatctttt tgttccatta aagggcgcga
     3421 ttgctgaata aaagatacga gagacctctc ttgtatcttt tttattttga gtggttttgt
     3481 ccgttacact agaaaaccga aagacaataa aaattttatt cttgctgagt ctggctttcg
     3541 gtaagctaga caaaacggac aaaataaaaa ttggcaaggg tttaaaggtg gagatttttt
     3601 gagtgatctt ctcaaaaaat actacctgtc ccttgctgat ttttaaacga gcacgagagc
     3661 aaaacccccc tttgctgagg tggcagaggg caggtttttt tgtttctttt ttctcgtaaa
     3721 aaaaagaaag gtcttaaagg ttttatggtt ttggtcggca ctgccgacag cctcgcagag
     3781 cacacacttt atgaatataa agtatagtgt gttatacttt acttggaagt ggttgccgga
     3841 aagagcgaaa atgcctcaca tttgtgccac ctaaaaagga gcgatttaca tatgagttat
     3901 gcagtttgta gaatgcaaaa agtgaaatca gctggactaa aaggcatgca atttcataat
     3961 caaagagagc gaaaaagtag aacgaatgat gatattgacc atgagcgaac acgtgaaaat
     4021 tatgatttga aaaatgataa aaatattgat tacaacgaac gtgtcaaaga aattattgaa
     4081 tcacaaaaaa caggtacaag aaaaacgagg aaagatgctg ttcttgtaaa tgagttgcta
     4141 gtaacatctg accgagattt ttttgagcaa ctggatccag gagaacaaaa acgatttttt
     4201 gaggaaagtt ataaattatt ttccgaacga tatggcaagc aaaatattgc ttatgcaaca
     4261 gttcataatg atgagcaaac ccctcacatg catttaggtg ttgtgcctat gcgtgatgga
     4321 aaactgcaag gaaaaaatgt gtttaatcgt caagaactgt tatggctaca agataaattc
     4381 cccgagcata tgaaaaaaca gggttttgag ttgaagcgtg gtgaacgtgg ctctgaccgt
     4441 aaacatattg agacagctaa atttaaaaaa caaactttgg aaaaagagat tgattttcta
     4501 gaaaaaaatt tagcagttaa aaaagatgaa tggactgctt atagcgataa agttaaatca
     4561 gatttagaag taccagcgaa acgacacatg aaaagtgttg aagtgccaac gggtgaaaag
     4621 tccatgtttg gtttgggaaa agaaataatg aaaacagaaa agaaaccaac caaaaatgtt
     4681 gttatatcgg agcgtgatta taaaaactta gtgactgctg cgagagataa cgataggtta
     4741 aaacagcatg ttagaaatct catgagtact gatatggcga gagaatataa aaaattaagt
     4801 aaagaacatg ggcaagttaa agaaaaatat agtggtcttg tagagcgatt taatgaaaat
     4861 gtaaatgatt ataatgagtt gcttgaagaa aacaagtctt taaagtctaa aataagcgat
     4921 ttaaagcgtg atgtgagttt aatctatgaa agcactaagg aattccttaa ggaacgtaca
     4981 gacggcttaa aagcctttaa aaacgttttt aaggggtttg tagacaaggt aaaggataaa
     5041 acagcacaat tccaagaaaa acacgattta gaacctaaaa agaacgaatt tgaactaact
     5101 cataaccgag aggtaaaaaa agaacgaagt cgagatcagg gaatgagttt ataaaataaa
     5161 aaaagcacct gaaaaggtgt ctttttttga tggttttgaa cttgttcttt cttatcttga
     5221 tatatataga aataacgtca tttttatttt agttgctgaa aggtgcgttg aagtgttggt
     5281 atgtatgtgt tttaaagtat tgaaaaccct taaaattggt tgcacagaaa aaccccatct
     5341 gttaaagtta taagtgacca aacaaataac taaatagatg ggggtttctt ttaatattat
     5401 gtgtcctaat agtagcattt attcagatga aaaatcaagg gttttagtgg acaagacaaa
     5461 aagtggaaaa gtgagaccat ggagagaaaa gaaaatcgct aatgttgatt actttgaact
     5521 tctgcatatt cttgaattta aaaaggctga aagagtaaaa gattgtgctg aaatattaga
     5581 gtataaacaa aatcgtgaaa caggcgaaag aaagttgtat cgagtgtggt tttgtaaatc
     5641 caggctttgt ccaatgtgca actggaggag agcaatgaaa catggcattc agtcacaaaa
     5701 ggttgttgct gaagttatta aacaaaagcc aacagttcgt tggttgtttc tcacattaac
     5761 agttaaaaat gtttatgatg gcgaagaatt aaataagagt ttgtcagata tggctcaaag
     5821 atttcgccga atgatgcaat ataaaaaaat taataaaaat cttgttggtt ttatgcgtgc
     5881 aacggaagtg acaataaata ataaagataa ttcttataat cagcacatgc atgtattggt
     5941 atgtgtggaa ccaacttatt ttaagaatac agaaaactac gtgaatcaaa aacaatggat
     6001 tcaattttgg aaaaaggcaa tgaaattaga ctatgatcca aatgtaaaag ttcaaatgat
     6061 tcgaccgaaa aataaatata aatcggatat acaatcggca attgacgaaa ctgcaaaata
     6121 tcctgtaaag gatacggatt ttatgaccga tgatgaagaa aagaatttga aacgtttgtc
     6181 tgatttggag gaaggtttac accgtaaaag gttaatctcc tatggtggtt tgttaaaaga
     6241 aatacataaa aaa
//
LOCUS       DACEJZ010000127         6136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEJZ010000127 DACEJZ010000000
VERSION     DACEJZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.132"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW656_002648"
     CDS             complement(207..1169)
                     /locus_tag="IW656_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0838321.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW656_002649"
     CDS             complement(1171..2610)
                     /locus_tag="IW656_002649"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0838322.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW656_002650"
     CDS             2795..4741
                     /locus_tag="IW656_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0838323.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IW656_002651"
     CDS             5005..5877
                     /locus_tag="IW656_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0838324.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1947 a         1055 c         1083 g         2051 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttcttc
//
LOCUS       DACEJZ010000128         6131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEJZ010000128 DACEJZ010000000
VERSION     DACEJZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.133"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1268)
                     /locus_tag="IW656_002652"
     CDS             complement(63..1268)
                     /locus_tag="IW656_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0838325.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IW656_002653"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IW656_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IW656_002654"
     CDS             complement(1447..2832)
                     /locus_tag="IW656_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0838326.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IW656_002655"
     CDS             complement(2861..3247)
                     /locus_tag="IW656_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0838327.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IW656_002656"
     CDS             complement(3263..3577)
                     /locus_tag="IW656_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0838328.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IW656_002657"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IW656_002657"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0838329.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IW656_002658"
     CDS             4837..6126
                     /locus_tag="IW656_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0838330.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         1773 a         1243 c         1154 g         1961 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa a
//
LOCUS       DACEJZ010000129         5926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEJZ010000129 DACEJZ010000000
VERSION     DACEJZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.136"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            690..1424
                     /locus_tag="IW656_002659"
     CDS             690..1424
                     /locus_tag="IW656_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0838331.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1440..2609
                     /locus_tag="IW656_002660"
     CDS             1440..2609
                     /locus_tag="IW656_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0838332.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2625..3620
                     /gene="lacD"
                     /locus_tag="IW656_002661"
     CDS             2625..3620
                     /gene="lacD"
                     /locus_tag="IW656_002661"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0838333.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3699..4625
                     /locus_tag="IW656_002662"
     CDS             3699..4625
                     /locus_tag="IW656_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0838334.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4644..5894
                     /locus_tag="IW656_002663"
     CDS             4644..5894
                     /locus_tag="IW656_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0838335.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1986 a          965 c         1209 g         1766 t
ORIGIN      
        1 aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccaaag tttgaaaaat
       61 gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag tgataataaa
      121 aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg gagaatacag
      181 agacctttaa aagggataag aaacaacata aattcctctt aagagagaac gaaaggacaa
      241 agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag actccctttt
      301 gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa gcaaatgcgt
      361 tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt catttgatag
      421 tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc agttgaagag
      481 agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat tatttttcat
      541 ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact aaaaatgaaa
      601 ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta tacttacatt
      661 gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca aaatcacttt
      721 attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc gctcatgaca
      781 agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg acggtgcgac
      841 tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga aaaggaacct
      901 ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt tttaccgaac
      961 aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag aagattgaag
     1021 caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta tttcaattag
     1081 accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat ttgccagtga
     1141 agctatttat gtccctttct caagaaattc tatttctgaa acctttatat aatttgtttt
     1201 cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc agcattgtcc
     1261 agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc catttagttc
     1321 gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta gcgcgggctg
     1381 ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga ggaaagaaaa
     1441 tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact acccgagaaa
     1501 ttcgacagca accggaactg tggcatgaga cgtttgaccg ttatcaagaa acagttgcag
     1561 atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca atccgagtca
     1621 tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg tttcttacta
     1681 gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat atcgttgcta
     1741 cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt gcccgtagtg
     1801 gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc aacaagatct
     1861 atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg cctacaaaaa
     1921 aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt gctatgacaa
     1981 gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca agcaatcagc
     2041 aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc caacgagaat
     2101 cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta ggttcgggaa
     2161 gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact gctggaaaaa
     2221 tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct tttgtcaatg
     2281 agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa tacgatgtag
     2341 acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga attggtcaaa
     2401 agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta ctgccggatg
     2461 gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt tgttcggtga
     2521 aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt gtggtcaaag
     2581 gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta gaatggtcgg
     2641 aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt accgctttgg
     2701 cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg gaagcgacaa
     2761 aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct tatgcctcag
     2821 caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca gcagcgagcg
     2881 gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc cgcttgccgg
     2941 atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat gcggtgaaat
     3001 ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag caagtttttg
     3061 ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta gagttattgt
     3121 catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca aaaccgcgca
     3181 aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat gtcttaaagg
     3241 ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag atcgcctata
     3301 cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat ttgccgtata
     3361 tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt tttgccaaac
     3421 atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa gatgcagtcg
     3481 ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa caagggacac
     3541 aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg tatgagaaag
     3601 taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga tatgtgtgat
     3661 tataatttat cattattgaa actgggagga tattttatat gaataaaaga ttgttagatt
     3721 attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca attttggctt
     3781 ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac atgggaaaat
     3841 cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg tttaatgcac
     3901 tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag aatccaataa
     3961 tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct gtagacaaag
     4021 aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt tctcctgaga
     4081 ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga aatccaggaa
     4141 caggagtatc taatagaact attgagaaag caataacatt atccaaatca atttttaatg
     4201 gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt atgaacttag
     4261 atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct gctccataca
     4321 ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata aaagatttta
     4381 atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga acaagccaag
     4441 actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca tgtggagctg
     4501 atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat atttttgctt
     4561 tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa tctaattatc
     4621 gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct gcaaaaatta
     4681 gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg ccattaatca
     4741 ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt acaaaatggt
     4801 tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca tttggtatta
     4861 ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat ggtgtagact
     4921 cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct ttattggatg
     4981 cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt tttgttggta
     5041 ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa aatattacaa
     5101 ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct attattcctg
     5161 gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa cttggaattg
     5221 aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta ctgacgaaaa
     5281 ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg tttggtattc
     5341 atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac tctgatgtaa
     5401 atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat gaattcttat
     5461 ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt ttaacatttt
     5521 tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct ccagtaagtt
     5581 ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata aaaatgttaa
     5641 ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca atgagtatgg
     5701 gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg ataattgccg
     5761 gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata acaattgcaa
     5821 taaatgtttt gatatactat ccattcttta aattagatga taatgccaag ctaaaaagcg
     5881 aaaaaaatga ttagaactac ctatatgttt ctaactgtag ttaagt
//
LOCUS       DACEJZ010000130         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEJZ010000130 DACEJZ010000000
VERSION     DACEJZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.137"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..667)
                     /locus_tag="IW656_002664"
     CDS             complement(95..667)
                     /locus_tag="IW656_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0838336.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW656_002665"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW656_002665"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0838337.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IW656_002666"
     CDS             complement(1352..1867)
                     /locus_tag="IW656_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ0838338.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IW656_002667"
     CDS             complement(1869..2153)
                     /locus_tag="IW656_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0838339.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IW656_002668"
     CDS             complement(2187..3515)
                     /locus_tag="IW656_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0838340.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IW656_002669"
     CDS             complement(3529..3984)
                     /locus_tag="IW656_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0838341.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5852)
                     /locus_tag="IW656_002670"
     CDS             complement(3987..5852)
                     /locus_tag="IW656_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0838342.1"
                     /translation="MAVSTKTIYQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVE
                     HLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRII
                     NKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSK
                     VISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRY
                     METYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLI
                     ERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVW
                     YAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVR
                     RFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIME
                     AYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELE
                     ANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRK
                     VKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2002 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taat
//
LOCUS       DACEJZ010000131         5887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEJZ010000131 DACEJZ010000000
VERSION     DACEJZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.138"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(437..610)
                     /locus_tag="IW656_002671"
     CDS             complement(437..610)
                     /locus_tag="IW656_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ0838343.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(953..1642)
                     /locus_tag="IW656_002672"
     CDS             complement(953..1642)
                     /locus_tag="IW656_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0838344.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="IW656_002673"
     CDS             complement(1705..2469)
                     /locus_tag="IW656_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0838345.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="IW656_002674"
     CDS             complement(2497..3195)
                     /locus_tag="IW656_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0838346.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="IW656_002675"
     CDS             complement(3207..3986)
                     /locus_tag="IW656_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0838347.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4002..4946)
                     /locus_tag="IW656_002676"
     CDS             complement(4002..4946)
                     /locus_tag="IW656_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0838348.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4992..5771)
                     /locus_tag="IW656_002677"
     CDS             complement(4992..5771)
                     /locus_tag="IW656_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838349.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
BASE COUNT         1590 a         1210 c          975 g         2112 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     4081 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4141 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4201 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     4261 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     4321 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     4381 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4441 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4501 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4561 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4621 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4681 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4741 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4801 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4861 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4921 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4981 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     5041 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     5101 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     5161 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     5221 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     5281 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     5341 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     5401 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5461 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5521 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5581 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5641 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5701 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5761 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5821 tacataaaaa tgacagtttt ttatttaaaa aaacataatt aaactaatat attcacagaa
     5881 atatttg
//
LOCUS       DACEJZ010000132         5579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEJZ010000132 DACEJZ010000000
VERSION     DACEJZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.139"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IW656_002678"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IW656_002678"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IW656_002679"
     CDS             complement(580..1353)
                     /locus_tag="IW656_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0838350.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IW656_002680"
     CDS             1497..1847
                     /locus_tag="IW656_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0838351.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IW656_002681"
     CDS             1828..2544
                     /locus_tag="IW656_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0838352.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IW656_002682"
     CDS             2544..3992
                     /locus_tag="IW656_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0838353.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IW656_002683"
     CDS             complement(4062..4571)
                     /locus_tag="IW656_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838354.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IW656_002684"
     CDS             complement(4561..5286)
                     /locus_tag="IW656_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838355.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1889 a          973 c         1119 g         1598 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgggctcttc gtcaatgagt gttggtgaag aaattcaata
     5521 gaataatcga gatactcaca cggctagaca gctatcttgt ctagccgtgt tgttgttct
//
LOCUS       DACEJZ010000133         5573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEJZ010000133 DACEJZ010000000
VERSION     DACEJZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.140"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(118..432)
                     /locus_tag="IW656_002685"
     CDS             complement(118..432)
                     /locus_tag="IW656_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0838356.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IW656_002686"
     CDS             complement(446..706)
                     /locus_tag="IW656_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0838357.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IW656_002687"
     CDS             876..1190
                     /locus_tag="IW656_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0838358.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IW656_002688"
     CDS             1192..2847
                     /locus_tag="IW656_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0838359.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IW656_002689"
     CDS             2930..5065
                     /locus_tag="IW656_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0838360.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..>5573
                     /locus_tag="IW656_002690"
     CDS             5240..>5573
                     /locus_tag="IW656_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838361.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         1891 a          962 c         1133 g         1587 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agattttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aat
//
LOCUS       DACEJZ010000134         5528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEJZ010000134 DACEJZ010000000
VERSION     DACEJZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.141"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..564
                     /locus_tag="IW656_002691"
     CDS             <1..564
                     /locus_tag="IW656_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ0838362.1"
                     /translation="QSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFT
                     NVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQP
                     KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSA
                     NRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(694..2004)
                     /locus_tag="IW656_002692"
                     /pseudo
     CDS             complement(694..2004)
                     /locus_tag="IW656_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
     gene            complement(2059..2235)
                     /locus_tag="IW656_002693"
                     /pseudo
     CDS             complement(2059..2235)
                     /locus_tag="IW656_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(2395..3432)
                     /locus_tag="IW656_002694"
     CDS             complement(2395..3432)
                     /locus_tag="IW656_002694"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0838363.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            complement(3429..3941)
                     /locus_tag="IW656_002695"
     CDS             complement(3429..3941)
                     /locus_tag="IW656_002695"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0838364.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            complement(3999..4265)
                     /locus_tag="IW656_002696"
     CDS             complement(3999..4265)
                     /locus_tag="IW656_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838365.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            complement(4941..5492)
                     /locus_tag="IW656_002697"
     CDS             complement(4941..5492)
                     /locus_tag="IW656_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ0838366.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT         1611 a         1049 c          814 g         2054 t
ORIGIN      
        1 caaagtggcg aaagcgaaga gacctggaca acattttttg aatacctaaa agaacgcggt
       61 ttacaaggta cggaactcgt tatttctgat gcgcacaaag gattagtctc tgccattaga
      121 aaatccttca ccaacgtaag ttggcaaaga tgccaagttc acttcctaag aaatatcttt
      181 accaccattc ctaaaaaaaa ttcaaaatct ttcagagaag ctgttaaagg aatttttaag
      241 ttcacagata ttaacttagc gcgtgaggct aaaaatcgat tgattcatga ttatatcgat
      301 caaccaaaat attcaaaagc ttgcgcatca ttggatgatg gattcgaaga cgcctttcaa
      361 tataccgtac aaggaaattc ccacaatcga ctaaagagta ccaatctaat tgaacgactg
      421 aatcaagaag tacgcagaag agaaaagatt attcgcatct tccccaatca aacatcagcc
      481 aatcgcttaa ttggagccgt tcttatggac ctacatgatg aatggattta ttcttcaaga
      541 aaatacatca attttgataa gtagaaatgg taaaaacatt gtatagcatt ttacacagga
      601 gtctggactt gactcacttc ctttattatt tttcattttt ttaaactatt taatactaat
      661 gtcttttata atagcttttc atatattata taatcaatct ttataagtcc ttttataaat
      721 ttcttttcta ccattttcga taaattcctg tttaatattt ttaattccat aaacaatagt
      781 ttcaatagga taatattctt caactatatc ttgatattct tttgctttct caatatctat
      841 atttccatac attcttaata tatcttctcc aaaatttgtt cctatttctt cttcactatc
      901 ttcaagtaag tatataaaat cacaatattc atctataatt ccagaatctc caaaatcaat
      961 tattccagtt aatctattat tgccatctaa caatagatga ttacaactaa aatcattatg
     1021 gcataaacac tttttaccct caaaaactgt tgttgcattt agtctttcca taaaactttc
     1081 tatataatct ttttctatat cagttaaatc attataaata gtttcacgca acaatatata
     1141 ctcttctaat acattttgtt tattatcaat agtacattca ctaatatctg tataatctaa
     1201 accgtgcatt tgtcttaaaa aactggcaat atctcgtttt aacaaatttt gttcttcttc
     1261 tgacatagta gaataaattt ctggtgttaa aaaagttcct ttaatttctt tataacctag
     1321 tatagataat tcatcactaa tatacgaata ttcaatatta ggaattttta cattagtttc
     1381 taaatttgta tttaaaaaat tatatattgc tttttctttt gcataacctt ttttcttatt
     1441 agtactaaat tttgttttaa aaatgtattc attattaact aaatatgcca cactatcata
     1501 accactaccg attatttcaa tactatctac tttgaaatta tcaaagtaat gctcaattaa
     1561 atatttcatt gccttaacat ttgtggcatt atcatcatat ctatattcca ttaaataaca
     1621 atcttctttt ttgccctcgt gtaattcatg ttctggcaaa tcttcaataa ttctaaaacc
     1681 agatttttgg tatgccctta ttgctcttgg attattttta tgagggtcta aaataactgc
     1741 attagcattt ctttcttttt tcaaaaattc aaaaatcaat ttaatatatc ttgtaccaat
     1801 tcctttactc caataatttg gctctcctat aaattgatcc ataccataga ctatctcatc
     1861 agtttttgga taatgataat cagtatataa ctcatcatac attttatata tttgtccata
     1921 tccaatagga acattgttat attcaataat tactctaaaa acttcatctt cccaaggctc
     1981 tgtataatgt ttttttaatg attcggttct gttgcaaagt tttaaataaa gaataaaatc
     2041 ccttacggta tctatgattt aagctgggat tcccaataat accttgattt cagtacagac
     2101 cgaaaacccg aagagagtgc cttcttttcg ggttttctta tataatcctc ggatggcttc
     2161 catgccttta atcgttgtag aggcagtgcg taaacttcga tagaatttat tgcgtctctt
     2221 tactggacga tggtcggtgt ttgtcaatat agttgtcgta tttttatttt ccacctatca
     2281 aaaagcagaa cctccctatg gtcggttggc ctcgttatcc aataggggaa gaataacttt
     2341 ttcccctatt gggtgacgag ggttatttga taatcttgaa agattatgct cctgttaact
     2401 ttctgtttct tttaaaggat tcaacgccac cgattgatgt ggagtccaat ttcgaacacc
     2461 ttttgaccag cgttcaggat ggcgcttttt agcgtcctcg taaacctgct ttcgtttctc
     2521 taaaatagcg agatgtcgtc cttcatgaca cgctgcagga gacacaaatt gaatcccact
     2581 atgcaaatga gaatcgttat accaatcgac aaactctttc gtccattttc gagcatcatc
     2641 tattgtttta aagccgttat tgggatattc tggtcgatat tttaacgttc ggaaagccgc
     2701 ttccgaataa ggattgtcat tactcactcg tggtcttgaa taagaacttt gaattcctaa
     2761 tttttcgagt aagacttgaa aagtcgcggc tttcataggg ctgccattat cggagtgtaa
     2821 aaccaacggc tgtccttgga tacgttcctt aagcaccgtc tgtttaataa gtttttcggc
     2881 atgacgagct tcttctgatt cccaaatctc ccagcctacc attttacggc taaataaatc
     2941 aatgatgaga tataaccggt aaaagagtcc ttttattgga ccttttagcc aagtaatatc
     3001 ccaagtccaa acttcatttg gcttcgtcgc aaggtgactt ggcggtatgc gtttttgtga
     3061 aacttgactt cggccacggt gatgttgcat atttttttct cgcaatacac gatagaagct
     3121 agactctgat gcaatataaa tcgagcaatc cgcaagtttt ggtacaattt ggctaggtgg
     3181 caaattcata aattcttttt ttgaaacggt ttctattatt ttttcgcgtt cttgatccgt
     3241 cagtttattt ttgggtacag gacgtttcgc caaagggcgt tgatcatgcg ctatcgtacc
     3301 ttcacgcgtc catcgttcgt aggttcgaac gctaatattc agctcattac aggcataggt
     3361 gagacgggcc ccattgcgat tggcttcttg aataagttca acagcttgtt tgcgatctga
     3421 agatgaaatc attcttcctc ttggtccccc caaatcgcgt gtgctttttt tcttagtaat
     3481 aagagagcag cggcttccgc taatgctttt tccttatgac gtaattcttt ctctaattga
     3541 cgcgcttgtt ttttctcttc ttttagttca gttgcaagtt ctctcgttcg attttcttcc
     3601 tgttcattcg cagataagca cgttttcttc caagcctcaa tttgttcttt aaataagccc
     3661 ttctttcggc aatattccgc aagttctact tggttcattg agaatgattc catcacaatt
     3721 aaaaatttat cttcacttga ccatttttcg acattttttt tcgttccggg tgtagtattc
     3781 ccttgaattc tagctttttt acgccaagaa caaagagtcg cttctggaat gcccaaagtt
     3841 tgacttaatt ggcgaatccc cctattttcg ggtggcatca ttttcttgat ggctgtcgct
     3901 ttcagttcgg tagagtaacg ttcattgttt ttatttgtca tggtaatcat cctccgtata
     3961 gtctttatta taacttatta tcctatacga caactagcct aacatagggg gtcttgtagt
     4021 gctttagctt taaattttgt ttgaagtata tcccaaagtc taatttgtgg atctaaactc
     4081 ataaaatcgg atagcttttt agcgctgttt ttattaaggt ttccgactat cgtcatagcg
     4141 tcaaagctca atttcggatt agaagtgcac acgaaatgtg cgtctaaccg gctattagag
     4201 tacgccggtt tttttgtgta attttccacc aaattttctt gctcattttt cgttattttt
     4261 gccataaaaa gcgactcctt aattttgttt aagaagtcgc ttttccaatt acatttgcga
     4321 atatatatct tgaagaataa tcttaaatcg tttaatatca agatatacaa ttaagaaaca
     4381 aacatcgact gtttattttg ctcacaaact ttggtcgggg agtgagcgac ttcttatttt
     4441 tattttgtta ttaatataac actaacaaaa gacttggtct aattagatca agtctttttt
     4501 agtaccaaaa cttattacat aatcaaaact taattctctt tagataattt atattgctgg
     4561 taagtataat aaactatcaa tgattgcact actacttgtg aaaaagctaa tgatactcga
     4621 atgattttct tcgttttctc actcatttta ctcaactcct ttcgttgctt tattccaatt
     4681 tccttattgg tcggaaccta cagggcttta tgtgttgttg agttggtact ttcttgggat
     4741 taatcccaat tcaagtccaa ccaactcgct aacaagttag ctaacacata gcccattcca
     4801 accaataagt tttctcggca taaaaacgtg ttataatact attagttgtt ttttcataag
     4861 gagttacccc cttatttgca tttattttgc attttaaggg ggtattttgc gtatttagcc
     4921 ctttttatat ataaatctac ctaaaggtca ttaaatactc taaaaaacaa tgctatgagc
     4981 cacagaatga tagcgaagaa tacatagggt actgcataaa gtatcaacat caaatatcgc
     5041 catgcacttt cgtggtcatc aataaaacca ttaataaagc tatataaatg ttcaatacca
     5101 aagaaatgac ctatcggacc tgtcacaagc attacaagta caaaaataat tagcacaaaa
     5161 aatatatttg taacgccttt tgtcatcact tttagcgctg tattcgattt atcaaccact
     5221 ttgttatggt cttttatttt actttctaag atttctttat actcttttgt ttcttctctc
     5281 gcttgtttcg tcacttcacg tatttcgtct ttagtatcgc ttaattcatc ttgaatagct
     5341 tgttgaaaat ctaatttcaa attatcttca tcaagtcgtt taatatagtg ctttgcagtt
     5401 gtttcaaagt ttaatcttgc atgcttagtc ttttcttcaa aaacatctaa cttattatta
     5461 aagttcgagg tcgaagcctc tagtcttttc atgagtatgt ttgttctctc gttcctgtct
     5521 tgctcgct
//
LOCUS       DACEJZ010000135         5504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEJZ010000135 DACEJZ010000000
VERSION     DACEJZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.142"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..342
                     /locus_tag="IW656_002698"
     CDS             91..342
                     /locus_tag="IW656_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838367.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            324..596
                     /locus_tag="IW656_002699"
     CDS             324..596
                     /locus_tag="IW656_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838368.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            763..1164
                     /gene="tnpA"
                     /locus_tag="IW656_002700"
     CDS             763..1164
                     /gene="tnpA"
                     /locus_tag="IW656_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0838369.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1176..2321
                     /gene="tnpB"
                     /locus_tag="IW656_002701"
     CDS             1176..2321
                     /gene="tnpB"
                     /locus_tag="IW656_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0838370.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2559..2822
                     /locus_tag="IW656_002702"
     CDS             2559..2822
                     /locus_tag="IW656_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0838371.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2816..3166
                     /locus_tag="IW656_002703"
     CDS             2816..3166
                     /locus_tag="IW656_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0838372.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3190..3804)
                     /locus_tag="IW656_002704"
     CDS             complement(3190..3804)
                     /locus_tag="IW656_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838373.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            4254..>5504
                     /locus_tag="IW656_002705"
     CDS             4254..>5504
                     /locus_tag="IW656_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0838374.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         1979 a          845 c         1033 g         1647 t
ORIGIN      
        1 ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct
       61 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ttatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatgttac atcatgaagt aatcagacgg attcaaaaag
      361 actttagttg tatggaagaa atgaagtact ttgttcaaaa tgttgaagaa cagttatatt
      421 tggaaagcga aacgagtcaa acaaaggtca cagtcagcca taatcgttta gatgtgtata
      481 aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct acgactagaa acagaaaaat
      541 acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga aaattttatg gactagccga
      601 gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta gctttagcta
      661 ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa taattgattt
      721 tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga aattcattat
      781 ggacggggtt atgtctattc tattcaatat catcttgttt ggtgtgtaaa atatcgacat
      841 gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca aattgctgat
      901 gataataata taaaaattat agaaatagaa tcagataaag accatattca tttactcatt
      961 gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg cgcatccgct
     1021 agactgctgt ttaaaaaaca tcctgaactt aaacaactcc tttggggtgg acatctttgg
     1081 aatccaagct actttgtagc tactgttagc gaaaatacag aagaacaaat tagaaaatat
     1141 atacaaaatc agaaaaagaa atgaggtgaa tccgtatgct acaacatagg gcttataaat
     1201 ttagaatcta tcctaatcaa gaacaagaaa tattaatgac taaaactatt ggctgttcaa
     1261 gatttgtcta taattatttt ttgaatttgt ggaatcatga atatacgact actggaaaag
     1321 gattatctta taattcttgt tctgccatgc ttccacaaat gaaaagaaat gcagagacaa
     1381 tttggttaaa agaagttgac agtattgcga ttcaatcctc attgaaaaat ctttctgatg
     1441 ctttttctcg cttctttaaa aagcaaaata aacgaccaca atttaaaagt aaaaaacatc
     1501 ctatacaaag ttatacaact aaaaatgtga ataatagtat cgaaattaga aacaatttcg
     1561 taaaactacc taagttagga tttgttaaat ttgcgaaaag tcaagagccc aaagggcgaa
     1621 tcttaaatgc tacgattcga aaaaattcga gtggtaaatt ttttgtttcc atcctgtgtg
     1681 aagaagaaat agttgagttt cctaaatcta attccgctat tggaatcgac cttggtataa
     1741 ctgattttgc aatcctttct gatggtcaaa agattgataa caataaattc acgtcaaaaa
     1801 tggcaaaaaa actcaaacgt gaacaacgta agttgtcaaa acgtgcatta ctagctaaaa
     1861 agaaaggcgt caatctcttt gaagctaaaa attatcaaaa acaaaaacga aaagtagcac
     1921 gactacatga aaaagtaatg aatcaacgca atgacttttt aaacaagttg agcacagaga
     1981 ttatcaaaaa ccacgatatt atctgtatag aagacttaaa tacaaaaggt atgttgcgta
     2041 atcataaact agcaaaaagc atttctgatg tgtcttggtc tagttttgtg actaagttac
     2101 aatacaaagc taactggtac ggtcggaaaa tcatcaaagt agataaatgg tttccatcta
     2161 gtcaaatctg ttcaaattgt ggacacaaag acggcaagaa atctctagaa attcgagaat
     2221 ggacttgtcc tgtttgtcat gctcatcatg accgagatat caatgctagt atcaatattt
     2281 tgaccgaagg tctaagatta catgtcatgg gattagctta gatccaatca tcaaaaaccg
     2341 taggaactac ggggatagct tggtaaataa gagaaaccgc tgtgagtaaa gaaatacgct
     2401 tgcaagtatg ctctattccc aagaatctcg tgactttagt catgagaggt tcaaggattc
     2461 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
     2521 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
     2581 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
     2641 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
     2701 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
     2761 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
     2821 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
     2881 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
     2941 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
     3001 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
     3061 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
     3121 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
     3181 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
     3241 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
     3301 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
     3361 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
     3421 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
     3481 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
     3541 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
     3601 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
     3661 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
     3721 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
     3781 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
     3841 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
     3901 taccttatat agacaaaaac taatgatgga ggaaatagta tgagaatttt ttgtaagtgt
     3961 ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa tattaaagaa
     4021 cggaactcca aaaaagtccg tactctaatt tgcagtctct cactctttta gagtgagagt
     4081 ttttattaga ttttcaaatt aattagtaaa gtgatagaat aaattgatca tacaaatcaa
     4141 aagaagaagc agggtgatag catacctatg cttctttttt tatgtattta gctatttctt
     4201 ctttacaata tcgaacatat gtttgtataa tgtgaaagaa tggagtgtta tttatgatgg
     4261 attattcaaa agagccagta aatgattact ttttaatcga tatgaaaagc ttttacgcaa
     4321 gtgtcgaatg tatagaaaga aatttagatc cattaacaac agaacttgtt gttatgagtc
     4381 gagctgataa tactggttca ggattgatat tagcttcttc tcctgaagca aaaaagcggt
     4441 atggtattac aaatgtgagt agaccacgtg acttaccaca accatttcct aaaacactac
     4501 acgttgttcc accacgtatg aacctatata tcaagcgaaa tatgcaggta aataatattt
     4561 tcagaagata tgtggctgat gaagatctac tgatttactc gatcgatgaa tcaatcttaa
     4621 aagtgacccg atcactgaat ctttttacga ctgaaggaac acgaagtcaa cggagaaaga
     4681 agctggctca aatgatccag gagaatatta aagaagagct aggattgatt gctacagtag
     4741 gtgtcggaga taatcccctt ttggctaaac tagccttaga caacgaagca aagcataatg
     4801 aaggatttgt tgcggaatgg acgtacgaaa acgttcctga aaaagtatgg aatattcctg
     4861 aaatgacgga cttttgggga attggctctc gaatgaaaaa aagactcaat cagatgggga
     4921 ttatgagcat tagagatctt gctaactgga atccttatac gattaagaat agactaggtg
     4981 tgattggctt acagctgtac ttccatgcta acggaatcga tcgaacagat atagcaatcc
     5041 cgccagaacc gacaaaggaa aaatcttatg gtaattcaca agtgttacca cgagattata
     5101 ctagaagaaa tgaaatcgag ctggtggtaa aagaaatggc ggaacaagta gccattcgta
     5161 tcaggcagca taattgtaaa acaggatgtg ttcatttaaa cattggtacg tcgatcttag
     5221 aaacgagacc aggattttca caccagatga aaatacctat cacagataat acaaaggaat
     5281 tgcagaatta ttgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     5341 ttggaataac ctatagcaag cttgtgtaca ccgattcgct tcagctggat ttatttagtg
     5401 atccacaaaa gcaaattaac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     5461 aatatggttt tacgtcgatt gtacatgctt cttcaatgtt agaa
//
LOCUS       DACEJZ010000136         5433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEJZ010000136 DACEJZ010000000
VERSION     DACEJZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.143"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..177
                     /locus_tag="IW656_002706"
     CDS             <1..177
                     /locus_tag="IW656_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838375.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IW656_002707"
     CDS             190..690
                     /locus_tag="IW656_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0838376.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IW656_002708"
     CDS             787..2634
                     /locus_tag="IW656_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0838377.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IW656_002709"
     CDS             2637..3533
                     /locus_tag="IW656_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0838378.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IW656_002710"
     CDS             3583..3972
                     /locus_tag="IW656_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838379.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5433
                     /locus_tag="IW656_002711"
     CDS             3959..>5433
                     /locus_tag="IW656_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0838380.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1856 a          784 c         1152 g         1641 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tgg
//
LOCUS       DACEJZ010000137         5015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEJZ010000137 DACEJZ010000000
VERSION     DACEJZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.144"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..2193)
                     /locus_tag="IW656_002712"
                     /pseudo
     CDS             complement(43..2193)
                     /locus_tag="IW656_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2339..2914
                     /locus_tag="IW656_002713"
     CDS             2339..2914
                     /locus_tag="IW656_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838381.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3128..3823
                     /gene="vanR"
                     /locus_tag="IW656_002714"
     CDS             3128..3823
                     /gene="vanR"
                     /locus_tag="IW656_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0838382.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3801..4955
                     /locus_tag="IW656_002715"
     CDS             3801..4955
                     /locus_tag="IW656_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0838383.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1568 a          940 c          971 g         1536 t
ORIGIN      
        1 tagtttgaaa tgcaattttc ttctttcagc tttttatttc ccacttgtaa atcggtagat
       61 ggtggctcct tttccagttc cataatgtgg atttgcatct gcatgtagtg atgaaacacc
      121 tagctgcatt ctcataccat ctgacgaaga tgttgtaccg tcgccccaat agaaaggcaa
      181 ttgtaattta tgatgaaagt ttactaatat ggcttgggct ttattcatgg catcttcata
      241 catgcgccat tgagatacat tggctagttg cttatatgta agtccgggtg tggcttcggc
      301 catcttgctc aagccaatat tcattcccat tcctaaaagg gcagccatga taatgattgt
      361 ttcttcctta tctggttttc gattattgga agcatgagtg aattgctcat gaaatcctgt
      421 tatatgggcc acatccatga gtaaatcagt taattttatt cttggtagca tctgataaag
      481 gcttgcacta aatttttttg cttcttctgg aacatctttt tctaagcgtg caagtgatag
      541 ctttcctttt tcaagagaaa ccccatctaa cttattggaa ttggcagcta accactttaa
      601 cctttcatta aagctgctgg ttctctccgt tatataatct tcgaatgata aactaactga
      661 taatctcgta ttccccttcg attgattcca tgtatcttcc gaaaacaaat attcctcaaa
      721 atccctatat tgtctgctgc caacaatgga aacatctcct gcccgaacat gctcccgaag
      781 ttctgttaaa acagccattt catagtaatg acgattaatt gttgtaccat catcctcgta
      841 taaatgtctt ttccatcgtt ttgaaataaa atccacaggt gagtcatcag gcacttttcg
      901 ctttccagat tcgttcattc ctcggataat ctcaacagct tgtaaaagtg gctcatttgc
      961 ctttgtagaa tgaaattcca atactcttaa tagcgttggc gtatattttc ttagtgaata
     1021 aaaccgtttt tgcagtaagt ctaaataatc atagtcggca ggacgtgcaa gttcctgagc
     1081 ctcttctact gaagagacaa aggtattcca ttcaataacc gattctaaaa ccttaaaaac
     1141 gtctaatttt tcctctcttg ctttaattaa tgcttgtccg atgttcgtaa agtgtataac
     1201 tttctcattt agctttttac cgttttgttt ctggatttcc tcttgagcct tacgaccttt
     1261 tgataacaaa ctaagtattt gcctatcatg aatttcaaac gctttatccg ttagctcctg
     1321 agtaagttgt aataaataga tggttaatat cgaataacgt ttattttctt gaaagtcacg
     1381 gaatgcatac ggctcgtatc ttgagcctaa gcgagacagc tgcaacaggc ggttacggtg
     1441 caaatgacta atttgcactg tttctaaatc cattcctcgt atgtattcga gtcgttctat
     1501 tatttttaga aaagtttcgg gtgaaggatg acccggtggc tcttttaacc aacccaatat
     1561 cgttttattg gattcggatg gatgctgcga ggtaataatc ccttcaagct tttctttttg
     1621 ctcatttgtt agagatttac taaccgtatt aaatagcttc ttttcagcca ttgcccttgc
     1681 ttcccacacc attctttcaa gtgtagtgat agcaggcagt ataattttgt tttttcttag
     1741 aaaatctatg cattcatgca gtagatgaat ggcatcacca ttttccaaag ctaattgatg
     1801 aaggtactta aatgtcattc gatattcact cagggtaaaa gttacaaagt cgtattcact
     1861 tcgaatttct ttcaaatgat cccaaagtgt attttccctt tgaggataat gatcaagcga
     1921 ggatggacta acaccaatct gtttcgatat atattgtatg accgaatctg ggatgctttt
     1981 gatatgagtg tatggccaac cgggataccg aagaacagct aattgaacag caaatcctaa
     2041 acggttttct tccctccttc gcttattaac tatttctaaa tcccgtttgg aaaaagtgaa
     2101 gtaggtcccc agtatccatt catcttcagg gatttgcata aaagcctgtc tctgttccgg
     2161 tgtaagcaat tctctacctc tcgcaatttt cattcagtat cattccattt ctgtattttc
     2221 aatttattag ttcaattata tatcaataga gtgtactcta ttgatacaaa tgtagtagac
     2281 tgataaaatc atagttaaga gcgtctcata agacttgtct caaaaatgag gtgatatttt
     2341 gcggaaaatc ggttatattc gtgtcagttc gactaaccag aatccttcaa gacaatttca
     2401 gcagttgaac gagatcggaa tggatattat atatgaagag aaagtttcag gagcaacaaa
     2461 ggatcgcgag caacttcaaa aagtgttaga cgatttacag gaagatgaca tcatttatgt
     2521 tacagactta actcgaatca ctcgtagtac acaagatcta tttgaattaa tcgataacat
     2581 acgagataaa aaggcaagtt taaaatcact aaaagataca tggcttgatt tatcagaaga
     2641 taatccatac agccaattct taattactgt aatggctggt gttaaccaat tagagcgaga
     2701 tcttattcgg atgagacaac gtgaagggat tgaattggct aagaaagaag gaaagtttaa
     2761 aggtcgatta aagaagtatc ataaaaatca cgcaggaatg aattatgcgg taaagctata
     2821 taaagaagga aatatgactg taaatcaaat ttgtgaaatt actaatgtat ctagggcttc
     2881 attatacagg aaattatcag aagtgaataa ttagccattc tgtattccgc taatgggcaa
     2941 tatttttaaa gaagaaaagg aaactataaa atattaacag cctcctagcg atgccgaaaa
     3001 gccctttgat aaaaaaagaa tcatcatctt aagaaattct tagtcattta ttatgtaaat
     3061 gcttataaat tcggccctat aatctgataa attattaagg gcaaacttat gtgaaagggt
     3121 gataactatg agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt
     3181 tgaattatac ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc
     3241 attggaatgt atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg
     3301 cacaagcggc cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat
     3361 gctgaccggg aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga
     3421 ttatataacg aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg
     3481 ccgatacaaa aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg
     3541 ccttgtcatt aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac
     3601 tcccaccgag ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc
     3661 cgagctgcta tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat
     3721 caccgtgcat atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata
     3781 tataaaaacg gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca
     3841 aactagaacg aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg
     3901 tgttgtatat tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg
     3961 aaaacaaata tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga
     4021 acaatataga tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg
     4081 tcatgctttc aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta
     4141 ttcagaacga agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc
     4201 tcaacacatt aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa
     4261 gaaaaaatga cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta
     4321 tcggttattt gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt
     4381 atgtgcatat cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg
     4441 agattacacg gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact
     4501 atatgctggt gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg
     4561 cggttattca cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag
     4621 tctttaacaa cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca
     4681 ttaccgcggg cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc
     4741 caaaagataa gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt
     4801 ccgatacggg tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg
     4861 gagggcagat ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag
     4921 cgatgccaga cttggttgat aaaaggaggt cctaagagat gtatataatt ttggctcttt
     4981 gtcaaatgat gttggtaaag agaaatctta gaata
//
LOCUS       DACEJZ010000138         4843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEJZ010000138 DACEJZ010000000
VERSION     DACEJZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.147"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            603..1166
                     /locus_tag="IW656_002716"
     CDS             603..1166
                     /locus_tag="IW656_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838384.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1445..1732)
                     /locus_tag="IW656_002717"
     CDS             complement(1445..1732)
                     /locus_tag="IW656_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0838385.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1722..2051)
                     /locus_tag="IW656_002718"
     CDS             complement(1722..2051)
                     /locus_tag="IW656_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0838386.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2356..2685
                     /locus_tag="IW656_002719"
     CDS             2356..2685
                     /locus_tag="IW656_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838387.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2972..3457)
                     /gene="vanZ"
                     /locus_tag="IW656_002720"
     CDS             complement(2972..3457)
                     /gene="vanZ"
                     /locus_tag="IW656_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0838388.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3610..4521)
                     /locus_tag="IW656_002721"
     CDS             complement(3610..4521)
                     /locus_tag="IW656_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0838389.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1581 a          832 c          733 g         1697 t
ORIGIN      
        1 tttgagtgta acataaatgg cttttttatt tgtctcgctt aattatacaa acggcgaagt
       61 taaaactttt ttagcagatt ttaaagtttc gtcaaaacta atatattctg gtagctgagc
      121 attcaaatag ctttcagact tcagaagaaa attacgagca tttttatagt ccatctctaa
      181 taaagttatt ctttttttat ccaatacatt cagctcaatt ccttataata ttctattcct
      241 tagtatctca acattattta ctttataaaa cgaataaatt ttgtagttct aattcaaata
      301 ttgcatgtta tatctaatat gggagcattt tataattttc attgtgataa actcgttatt
      361 aactttagga tacactagtt aggattaaat taggtatcta gaaatttact taagattttt
      421 ttgtttattt cgtatacaca ctcgtatcaa aaacaatcga ttatgagaca aaatgaaatg
      481 acctctttta ttaaataaaa gaggtcatat tttgtatcat aacttgttgc attaactatg
      541 tatctataac tcccttttat gatacaatca agccagaatt aaagtacgaa aggaagaaaa
      601 gcatgagtgg atacatttat ggttatgccc gagtgagtac acgttcacaa gaactaaatc
      661 gtcaattaga cttactcaat gaacaaaatt gtaatgaaat cctaactgag aaaatgacgg
      721 gaaccaaagc ggttcgtccc caactaaatc gtttaaaaga taaaattcgt ccgggagata
      781 cggtaattgt agaaagtttt tctcgattgg gtcgtagtac aaaggatttg attgaccttg
      841 ttacctattt tgaagaacac catgttaagc tggtgagctt gaaagaaaac ttcgatacca
      901 gcacgcctca cggacgctta atgatgactg tcttccaagc atttagtcag ttcgaacgtg
      961 atttaattgt tgaacgtacc aaagaaggtt tgaaaagtgc acgtgcgaga ggtcgtaaag
     1021 gtggccgtcc acgagtgaac cagaaagatg ttgatcgagc agtaaaattg tataaaagtc
     1081 aggtttatag cgtaaaagaa attacggaaa tgactggtat tagcaaggca acactctacc
     1141 gctacttaaa agacaatagg gaataatcta ttttaatctt taggaatcta taatatttat
     1201 gggagaatta gagtgatttt tttattatta ctaaaaaaca atataaaata acgcatttta
     1261 agccctcttt gtgcataagg atgaagcttt taatttgaaa gaggcttaaa atgcgtttta
     1321 attttttcta tttttcataa atatataggt tagtggttta aaattatagg gtattacaac
     1381 tctcagctgt taaaaattat cttttaaagt gtcgtctatc ctctcaagtt gaaacattgg
     1441 gttcttacct aatctttttt aattgttcat aaaaattttc atgcgttcca catagtaaga
     1501 taggtatcac tgcattatct ttaatttcat atgcaacgcg atatgtcgta cctgcatact
     1561 tgaatccaat agcccaaatt cctgataaat cacccgtttt ttgttctccc gaatgaggac
     1621 gaactgctat ctcatcataa attatcgtta aaaattgttc ttttaatgtt ttctctttta
     1681 atttttttag atactttatt acgtgtttag aaggtagaat ctcataagcc aatttctgct
     1741 gccaactcct cttttgtcat cggtttagaa tttgcaacag tatcttcagc aatagattta
     1801 aatgcagaag ttaacgcatt ttttctattt ctaaattctt ttaagatctc ttgtttgcta
     1861 taaccttctg aaagaatatc agccaaaata tcttcactaa aatcaaaaaa ttctttttta
     1921 ggctctacaa actcataaag gataccattc tctacaagct tagcttctac ttcaacacca
     1981 ttgggcacat cgaattcttt agggaccgtt aacataatag aatttccttg ttttcttgtt
     2041 tttgtaatca tattatttcc tccattattt tacatttcgt aattacttag taattacatt
     2101 gtacgctatt tttagtagaa atacaaaata ataaaactcc tttagaacaa ttttcaaagg
     2161 agttttattt gattaggaaa ttatgtagaa taataatatg tttttaactt ggaatcaaaa
     2221 acgcacttgc aagtaagttt acttcaatac atgaaattgc ttataatagt gtattggaat
     2281 taaggtagat tgacaaaaga gtgatcaaat aggtatactt cattcaaata atcgtttgaa
     2341 tgtgaggtga gatgaatgaa aaaagtttgc caagtgactg tcgtacataa agaaaaagta
     2401 aatggaataa aaaataaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     2461 tgtttcagcg atttttctag aattaaaatt ttctatgctt tagaaaccta taaggaaatg
     2521 tgcgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     2581 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattac
     2641 tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc
     2701 tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg
     2761 aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta
     2821 ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg
     2881 ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt
     2941 atgaaagatc agattgcttg atttagacat cttacacgta atttattctt aaatgggtac
     3001 ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt
     3061 ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa
     3121 cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta
     3181 tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt
     3241 taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct
     3301 ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa
     3361 atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat
     3421 ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg
     3481 tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc
     3541 taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt
     3601 ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac
     3661 accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt
     3721 atttttagta acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat
     3781 ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat
     3841 aatcgcacta tgtggtaaac caacatagcg aatatgccat ggttcatatt gaattcctgt
     3901 taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc
     3961 tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga
     4021 taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg
     4081 gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac
     4141 gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat
     4201 ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt
     4261 cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag
     4321 atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc
     4381 actattttct aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg
     4441 agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa
     4501 taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg gaagtatgaa
     4561 atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg ccgttttgcc
     4621 ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc aatgtagatg
     4681 aattttcata agtgataccc atgctgaatt taacgaggct gaactgcctg ttgacaatgg
     4741 gcggtgtgaa ataccacccc tctatgcgat cagtcttatt atcaggctat cttgccgcag
     4801 cgcacgcaga taatgaccgc aatatcgctc tgccttacat ctt
//
LOCUS       DACEJZ010000139         4616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEJZ010000139 DACEJZ010000000
VERSION     DACEJZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.148"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..962)
                     /locus_tag="IW656_002722"
     CDS             complement(15..962)
                     /locus_tag="IW656_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0838390.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="IW656_002723"
     CDS             complement(1140..1943)
                     /locus_tag="IW656_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0838391.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="IW656_002724"
     CDS             2089..3003
                     /locus_tag="IW656_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0838392.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="IW656_002725"
     CDS             3051..3521
                     /locus_tag="IW656_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0838393.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="IW656_002726"
     CDS             3606..4214
                     /locus_tag="IW656_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0838394.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="IW656_002727"
     CDS             4348..4548
                     /locus_tag="IW656_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0838395.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttag
//
LOCUS       DACEJZ010000140         4574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEJZ010000140 DACEJZ010000000
VERSION     DACEJZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.149"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1618)
                     /locus_tag="IW656_002728"
     CDS             complement(98..1618)
                     /locus_tag="IW656_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0838396.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1714..1839)
                     /locus_tag="IW656_002729"
     CDS             complement(1714..1839)
                     /locus_tag="IW656_002729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0838397.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(1861..2046)
                     /locus_tag="IW656_002730"
     CDS             complement(1861..2046)
                     /locus_tag="IW656_002730"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ0838398.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2030..2212)
                     /locus_tag="IW656_002731"
     CDS             complement(2030..2212)
                     /locus_tag="IW656_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838399.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2453..2671
                     /locus_tag="IW656_002732"
                     /pseudo
     CDS             2453..2671
                     /locus_tag="IW656_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3166..3765
                     /locus_tag="IW656_002733"
     CDS             3166..3765
                     /locus_tag="IW656_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0838400.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3829..4428
                     /locus_tag="IW656_002734"
     CDS             3829..4428
                     /locus_tag="IW656_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838401.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGDPVLLQSFK"
     gene            complement(4458..>4574)
                     /locus_tag="IW656_002735"
     CDS             complement(4458..>4574)
                     /locus_tag="IW656_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0838402.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1451 a          835 c          815 g         1473 t
ORIGIN      
        1 taattcagta gttttgagcg taagcgtgtc atagaatggt acctaaacac aaatagcccg
       61 atttgataca ctcaaatcag gttatttgtg gcttatatta tttacagatt tcttgtatct
      121 gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt catcgaagac agattgggaa
      181 tctctgtaaa aagatgattt aaatatttgc ggatgtttaa cccattcgca acagctgtct
      241 ggataatact caatataatt ccatttgatc tagcgccttt taagctttta gaaaatagcc
      301 agttttttcg accgatcact aactctttga tggcacgctc tgctcggtta tttgaaagtt
      361 ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt atgattgact gcgtattgaa
      421 ccgcctttcc aagctgcgat tttggcaaat aagcaaaact ctcacaccaa tgaaaaaatt
      481 cagtcatcac aggctgcaac gtttccttcc tcgctacaaa cctttcttct gcgctaagct
      541 tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa gtctaatcct attttggcat
      601 gactttttgg ggacggttgt ttacccaatg cttcaaaaaa ctttctgcga acatgcgccc
      661 aacaataaac attgtttacc ggcagctttc cataggccgc gtaaccatca gtatgcaaga
      721 aaccgctaaa tgattctaaa aaactttttg gctcatcaac ggaacgcgaa tttgcgtgct
      781 cgtagagcac aacttgtttt tcttcgcttt tacctgaagc atataaccag taaaaggtct
      841 tttctttatg agtattcaat acccgatagc ttgtctcatc cgcgtgtaag acctcctgat
      901 tgcttaattc ttgatgaagg caattgtaga ttggctctaa aaccatttca cttaccttga
      961 tatgccaatt agtgattttt ttacgatcaa gagggaaacc aagtgcatgc cattcttttt
     1021 cttgtcgata tgccggcacc tttttaacaa atttttccca aatactctgt gtcacaattg
     1081 aagaggaacc tagactatta ttgatcaacg gtttaggaac aggcgctttt ttgatttttg
     1141 tttctcctaa tgcttcacat tctctgcaat gataggcatg ttggtagatg acttgttttt
     1201 ctatatgggc aggaacatac aatggctctt ctctaagcct tgtcgcaccg atatcatgta
     1261 aagtatgacc acagtcttca cacaaacaat cggcaccttt taattcataa tgaataggaa
     1321 cttcaggcag accttctaat gaaagtgctt tgagtccctt gcgttttctg cgtttaaaag
     1381 aagataccgt aatgggttcc tggctttctt gtccagtgtg ctctggttca ttaaaagttg
     1441 agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt ttcttttttt cgtccaaaca
     1501 agcgtcgatt catttcagca atttgttctc taagaagagt attttcctgt tgctggattt
     1561 caagcaactg tgttaattgc ttgatttgta caagaagttc agattccatt tttgtcattt
     1621 ttacaccacc attctagcga ttttcttaca tctattatac cattttatga caacgaaaaa
     1681 cagacaaaat ccttgattta acaaggtttt cgtctaatat atcgcacctt ttttcgtttc
     1741 ttttagcgta catttttctt cgattgaaag gccagtcagt aagacttctt ttctggaacg
     1801 cggccattga aggtacccac tttcaaatct tttgtacatt aatacaaatc cattttgatc
     1861 ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa aataagaaaa gcgcttgatc
     1921 gtaaacatca aggtcatatt cttctataac gaaagctgct agtccatcaa ttccttttcg
     1981 aagatctgtt tttccacaaa cgataaaaat atgtttcaat gcagttgcat caatgagcat
     2041 tggcattcaa ctcttttaac aaggtataca aaagatattt atccattcca ttataaataa
     2101 atatttcctt atcttccgat ttgattctgc aagctagttt tggcttggtg tcaatttgcg
     2161 atttttgagc cgcacttgaa aattgaactg gtacaatatc ttttttattc attcgttttt
     2221 ctccttctat atttgatagt ttaattatag aagaaagata atcggcgtac gagatactat
     2281 cctatgacac gcttacattt aatttaatat tttgcctcaa attagccaca ctttttatag
     2341 aactaagagg aaaaatgaag ttttcttcct attaatatag ttgtttgaaa tcttaaatat
     2401 agcgttttac gatgtaatgg gaagtatggt gaataaatca actatgttcg tacgaagcat
     2461 ttactcaact aatttaatcg aatctttcaa taaacaaatc aagaaataca gccaccgcaa
     2521 ggaacagttc caaaatgaag agtcgatgga acgtttctta gtctcgtctt ttgatactta
     2581 caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa caggccgaag gcgagctcga
     2641 acaaatgctg agtcaaccga tggagaatta gaaacaatct acagtaggga agaaccattg
     2701 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
     2761 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
     2821 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
     2881 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
     2941 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
     3001 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
     3061 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
     3121 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
     3181 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
     3241 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
     3301 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
     3361 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
     3421 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
     3481 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
     3541 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
     3601 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
     3661 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     3721 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     3781 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     3841 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     3901 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     3961 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     4021 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     4081 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     4141 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     4201 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     4261 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     4321 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     4381 tttgacaact ttataggaga tccggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4441 cttacggtat ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc
     4501 gaaaacccga agagagtgcc ttcttttcgg gttttcttat ataatcctcg aatggcttcc
     4561 atgcctttaa tcgt
//
LOCUS       DACEJZ010000141         4517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEJZ010000141 DACEJZ010000000
VERSION     DACEJZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.150"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..238
                     /locus_tag="IW656_002736"
     CDS             <1..238
                     /locus_tag="IW656_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838403.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IW656_002737"
     CDS             252..440
                     /locus_tag="IW656_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838404.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IW656_002738"
     CDS             452..1063
                     /locus_tag="IW656_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ0838405.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IW656_002739"
     CDS             1048..2439
                     /locus_tag="IW656_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0838406.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IW656_002740"
     CDS             2655..2957
                     /locus_tag="IW656_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838407.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..3297
                     /locus_tag="IW656_002741"
     CDS             2950..3297
                     /locus_tag="IW656_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838408.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
     gene            complement(3357..>4517)
                     /locus_tag="IW656_002742"
     CDS             complement(3357..>4517)
                     /locus_tag="IW656_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ0838409.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYK
                     LTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         1638 a          694 c          906 g         1279 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa aggctcttcg tcaaatcgtg ttggtgaaga
     3301 caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgccgca tattttttag
     3361 ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc gaaagttata aaagttgcga
     3421 taaccaaacg caattcgttt aatcagcttg atcttgttat tcagcccctc agtgacgccg
     3481 tttgagtggg acaccttaaa ggcatttgaa atacccgatt ggtagcgatg gaagatcgtg
     3541 aatttctttt taaacgactg aggtaacgta gatgaaattg ttttaagggt ttcttgaaag
     3601 ccttccgtac ctcgtttaac gaaataatag cgtaataatt gacaaaaatg atacgcagtt
     3661 cgcaattctt cgttgtagct taacagctca tcaacgattt ctgtttgact aattggtcgt
     3721 ttaaacagtc gatgataatg ccgtttgagt ggctccaatg tcgtggaatc tttgagtaag
     3781 agtttccaat atcgcttgag ccgacggtac ttcttctgat tttcagagtg tgaggttcga
     3841 aaacgattca tgatctggac acgtaattga ttgaagctgc gattgatgtg ttggacaata
     3901 tgaaagcggt cagttacgat ctccgcattt ggaaagactg ttttaagcaa ctgacaatag
     3961 ctggcattca tgtccatcac taggtacttg acctttaaac gggcttttct aggaaagtgc
     4021 aggaagtatt gagttagttt gtaaagtctg cgatcttcaa ggatcgtaaa tagctgattg
     4081 gttgtcccat caacacaaat aaaactcatg gaaccagaaa cagacttcat tgatttgaac
     4141 tcatccatac acaacaccgt cggaaggaag ttcatacgtg gctggtaatg cttcgttgct
     4201 tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt acttcttagc gattagttta
     4261 cgtgattgat tttcgtacag ttccatgaga attgcctgct tcagttcctt tgttagttga
     4321 tgatgttcat caactaagtc cgttttcgca gaaaaggtcc ttccacaatc atggcagaga
     4381 aaacgggtac gttttaattc taaaatagtt aacttttctt tgactttcaa catctgagtg
     4441 cgtgtcgtat aggaaccgtt gcggataata cgaaccgatc gacagtgttc acaataggaa
     4501 ggagagtcat ccaattt
//
LOCUS       DACEJZ010000142         4446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEJZ010000142 DACEJZ010000000
VERSION     DACEJZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.151"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW656_002743"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW656_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0838410.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IW656_002744"
     CDS             complement(2944..3378)
                     /locus_tag="IW656_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838411.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4446)
                     /locus_tag="IW656_002745"
     CDS             complement(3395..>4446)
                     /locus_tag="IW656_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0838412.1"
                     /translation="IKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFK
                     LKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYT
                     ENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIP
                     SVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKV
                     RDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRES
                     FKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSP
                     KQYQLGLVDNFNNIS"
BASE COUNT         1376 a          825 c          612 g         1633 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatat
//
LOCUS       DACEJZ010000143         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEJZ010000143 DACEJZ010000000
VERSION     DACEJZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.152"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW656_002746"
     CDS             complement(543..2048)
                     /locus_tag="IW656_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0838413.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW656_002747"
     CDS             complement(2045..2731)
                     /locus_tag="IW656_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0838414.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW656_002748"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW656_002748"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0838415.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACEJZ010000144         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEJZ010000144 DACEJZ010000000
VERSION     DACEJZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.153"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW656_002749"
     CDS             complement(83..1051)
                     /locus_tag="IW656_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0838416.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW656_002750"
     CDS             complement(1498..2904)
                     /locus_tag="IW656_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0838417.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKQGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW656_002751"
     CDS             complement(3126..3488)
                     /locus_tag="IW656_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW656_002752"
     CDS             complement(3492..3800)
                     /locus_tag="IW656_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW656_002753"
     CDS             complement(3809..4228)
                     /locus_tag="IW656_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW656_002754"
     CDS             complement(4221..4361)
                     /locus_tag="IW656_002754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838421.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          993 c          619 g         1528 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ctgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEJZ010000145         4154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEJZ010000145 DACEJZ010000000
VERSION     DACEJZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.155"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            144..299
                     /locus_tag="IW656_002755"
                     /pseudo
     CDS             144..299
                     /locus_tag="IW656_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            318..1595
                     /locus_tag="IW656_002756"
     CDS             318..1595
                     /locus_tag="IW656_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0838422.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1605..2261
                     /locus_tag="IW656_002757"
     CDS             1605..2261
                     /locus_tag="IW656_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0838423.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2261..3637
                     /locus_tag="IW656_002758"
     CDS             2261..3637
                     /locus_tag="IW656_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0838424.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3767..>4154
                     /locus_tag="IW656_002759"
     CDS             3767..>4154
                     /locus_tag="IW656_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0838425.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQL"
BASE COUNT         1642 a          460 c          734 g         1318 t
ORIGIN      
        1 gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct tcgttgacgt
       61 cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta ttttttctgt
      121 ctagtctagt gtagccgatt caggataagg ataagtgtat taattgtgga aaatgtaaaa
      181 aagtttgcca aatgaataaa gattcagttg agaattgcaa tcatctggag tgtatcagat
      241 gtggacgatg taaaaatgct tgtccagtgg atgcaattag ttatggagta agaaaataga
      301 gaggagtagt taggaaagtg ataaaaaatg ctatagcata tattacaaga aagaaaaata
      361 gaactttaat aatttttatc atattaacta tagttctttc ttgcttgtat tcttgtttaa
      421 caataatgaa atcaagcaat gaaatagaaa aggctttata tgaaagttct aattcttcaa
      481 tatcaataac aaaaaaagat ggtaaatatt ttaatgttaa tcaatttaaa gatattgaaa
      541 aattaaaaga aattgaagaa aaaataattc aatatgatgg attagcaaaa ttaaaagatg
      601 ctaaagtagt tagcggagaa caaagaataa atagagaaga tttatctgac gaatttaaga
      661 atgttgtttc actcgaagct acaaataata ctaaaagaaa tattttattt agtagtggag
      721 tatttacaat taaagaaggt aaaaatatag aagaaaatga taagaattca attattgttc
      781 atgaagaatt tgctaaacaa aacaatctaa aattaggtga tgaagttgat cttgaattac
      841 tagatattga aaaaagtgga aaaataaaaa gtcataaatt taaaattata gggatctttt
      901 ctggtaaaaa acaggaaaca tatacaggat tatcttctga ttttagtgaa aatatggtgt
      961 ttgtagatta ttcaacaagc caagagatat taaataattc agaaaataat gaaattgcaa
     1021 ataaaatttt aatgtattct ggtagtgcag aatctacaga cttagcctta aacaaattga
     1081 aagagcttaa aattgatgag tcaaagtatt ttgttgaaaa agattctaat gcgtttgaag
     1141 agtctttaga gtcagtgagt ggaataaaat atataataaa agtaatgact tattctatta
     1201 tgttaggtgg gatggttgtt ctttcattaa tcttgattct atggttaagg gaaagaattt
     1261 atgaaatagg tatattctta tctattggaa catctaagat acaaattata atgcaattta
     1321 tattcgagtt aatattcata tcgataccaa gtataatatc ttccttattt ttaggaaatg
     1381 tattactaaa agtaattgta gatggattca ttaactcaga ggattcaatg atttccggtg
     1441 gaagtttaat aaataatagt agttttatgt caaatataac aacacttgga caaagttatt
     1501 taatattaat aagtattatt gttttatcag ttgtatttgc ttcttcatta atgttaatca
     1561 agaagcctaa agaaatctta tcaaaaataa gttaggagaa aataatggat atattagaaa
     1621 taaagaatgt agcatatagt tatgcaaatt ctaaagaaaa agttttgtca ggagtaaatc
     1681 aaaaatttga acttgggaag ttttatgcga tagtaggaaa gtcaggaaca ggaaaatcta
     1741 cacttctttc cttacttgct ggacttgata aacctcaaac aggaaaaata ttgtttaaga
     1801 atgaagatat acaaaataaa ggatatagta atcatagaaa aaataatata tctttagtat
     1861 ttcaaaacta taatttaata gattatttat caccaattga aaatattaga ttagtaaata
     1921 aatcagcaga tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa
     1981 atgttatgaa attatctggt ggacagcaac aaagagtagc tattgctaga gcattggtat
     2041 cagatgctcc aataatacta gctgatgagc ctactggtaa tctagacagt gttactgctg
     2101 gagaaataat taatatatta aagacattag ctaaagatag aaataaatgt gtaatagttg
     2161 taacacatag taaggaagta gcagattctg cggatatcat tttagaacta agtggtaaaa
     2221 agttgaaaaa agtaaataaa atgaatttgg aggttgaata atgataaaaa atgcatttgc
     2281 ttatgtaact agaaaaagtt taaaatcatt aattatcata ttagttattt tatctatgtc
     2341 aactttaagt ctcattagtt tatctattaa agatgctacg gacagagctt caaaagaaac
     2401 atttgctaat ataacaaata gtttttctat ggagataaat agacaagtaa atccaggaac
     2461 acctagaggt gggggaaatg taaaaggtga agatattaaa aagatatctc aaacagatag
     2521 tatagactcc tatgtaaaaa gaataaacag tgttgcagat ttagttgatt atgatattat
     2581 tgaaactcaa gagactcttg cgaatcaatc acctgaaaga gcaaagaatt ttaaaagaac
     2641 agttatgtta actggggtta acgactcaac aaaagaaaca aaatttgtat cagaagcata
     2701 taaattagta gaaggaaagc atttagaaaa tgaagataaa aataaaatct taatgcataa
     2761 agatttagct gaaaaaaata accttaaagt tggagataag ataaaaataa aatctaattt
     2821 atttgatgct gataatgaga aaggtgcaga tgaaacagta gaagtagaaa ttaaaggtct
     2881 attcgatggg cataatagcg gtgtagtaag tgcagcacaa gaactatacg aaaatacatt
     2941 aatcactgat attcatacag ctgctaaagt ttatggtaat accgaagata cagctgtata
     3001 ccaagatgca acattctttg taaaaggtga taagaatctt gatagtgtaa taaaagatct
     3061 tgggaaatta gatataaatt ggagagaata taatttgatt aaaagttcat caaattaccc
     3121 tgcattacaa caatctatat caggtattta ctcaatttca aataaattat tttttggctc
     3181 attaatattt gcaggagttg tagtttcatt attattattc ttatggatga atgctagaaa
     3241 gaaagaaata gcagtattac tatcattagg tatatcaaaa ttagaaattt ttggtcaatt
     3301 cctaattgag atggtattta tatcaatccc cgcattcgtt ggttcctatt tcttagctca
     3361 atatacagct gacaagcttg ggaataatat attgaataag gttactggtg atatagctaa
     3421 acaaatagct aaacaatctg catctagcca attaggtggt ggggcagaag ctgaaggatt
     3481 taacaagaca ttatcaagtt tagatatcaa tgtattacct aaattcatga tttatgtagt
     3541 tatatttatg agtttagtac ttcttgtatc attgattatt tcttcattca atatattaag
     3601 aaggaatcca aaagaattat taattgacaa taattaaaat tttcggtgct ttttagcacc
     3661 aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca atcctaggcg ggtggttgct
     3721 tcacggtttt tatttttcta aaataagatc aagaggtgat tacacgatgg ctagaaagaa
     3781 aagaaatcct gatgctgaga agttagctga atccattttg aatacttatc aacccgaatc
     3841 tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt cccttatttg aaaaaatgct
     3901 tcaaggagaa ttgaataacc atttagggta tgacgcccat tctaaagagc caaagaaaca
     3961 tgacaatcga cgaaacggtt atggcaataa gacgcttaaa acgagctttg gtaaagtaaa
     4021 cattgatgtt cccagagacc gggaggcttc ttttgaacct gagttgattc ctaatagaaa
     4081 acgagatgtc tccgacatcg cagactgtag acaaaagctg ttccaaactc gtaactcgag
     4141 cttggaacag cttt
//
LOCUS       DACEJZ010000146         4038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEJZ010000146 DACEJZ010000000
VERSION     DACEJZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.156"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW656_002760"
     CDS             179..775
                     /locus_tag="IW656_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0838426.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW656_002761"
     CDS             899..2698
                     /locus_tag="IW656_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0838427.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW656_002762"
     CDS             complement(2976..3188)
                     /locus_tag="IW656_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838428.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1186 a          689 c          712 g         1451 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaat
//
LOCUS       DACEJZ010000147         4035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEJZ010000147 DACEJZ010000000
VERSION     DACEJZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.157"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(177..1463)
                     /locus_tag="IW656_002763"
     CDS             complement(177..1463)
                     /locus_tag="IW656_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0838429.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1456..2724)
                     /locus_tag="IW656_002764"
     CDS             complement(1456..2724)
                     /locus_tag="IW656_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0838430.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2976..3437
                     /gene="mscL"
                     /locus_tag="IW656_002765"
     CDS             2976..3437
                     /gene="mscL"
                     /locus_tag="IW656_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0838431.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3496..3942)
                     /locus_tag="IW656_002766"
     CDS             complement(3496..3942)
                     /locus_tag="IW656_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0838432.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1138 a          821 c          711 g         1365 t
ORIGIN      
        1 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
       61 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
      121 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
      181 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
      241 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
      301 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
      361 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
      421 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
      481 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
      541 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
      601 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
      661 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
      721 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
      781 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
      841 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
      901 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
      961 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
     1021 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
     1081 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
     1141 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
     1201 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
     1261 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
     1321 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
     1381 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
     1441 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
     1501 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
     1561 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
     1621 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
     1681 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
     1741 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
     1801 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
     1861 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
     1921 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
     1981 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
     2041 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
     2101 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
     2161 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
     2221 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
     2281 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
     2341 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
     2401 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
     2461 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
     2521 ataaagctct gcaagttgcg cacttaactt tgtctgatcc ggataattca aactatttgt
     2581 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
     2641 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
     2701 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
     2761 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
     2821 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
     2881 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
     2941 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
     3001 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
     3061 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
     3121 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
     3181 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
     3241 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
     3301 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
     3361 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
     3421 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
     3481 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
     3541 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
     3601 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
     3661 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
     3721 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
     3781 atcagtatct tgaaaaaacg tatgccaagc ttgtccatca atgtcatcaa cttcttgata
     3841 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
     3901 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
     3961 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
     4021 ggctcgtttg ctttt
//
LOCUS       DACEJZ010000148         3959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEJZ010000148 DACEJZ010000000
VERSION     DACEJZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.158"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(559..1599)
                     /locus_tag="IW656_002767"
     CDS             complement(559..1599)
                     /locus_tag="IW656_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0838433.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="IW656_002768"
     CDS             complement(1948..2274)
                     /locus_tag="IW656_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838434.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="IW656_002769"
     CDS             complement(2261..3049)
                     /locus_tag="IW656_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0838435.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="IW656_002770"
     CDS             complement(3417..3707)
                     /locus_tag="IW656_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0838436.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         1227 a          660 c          556 g         1516 t
ORIGIN      
        1 cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct taatgctttc
       61 ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct ttcttttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataacccca
//
LOCUS       DACEJZ010000149         3957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEJZ010000149 DACEJZ010000000
VERSION     DACEJZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.159"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(607..1164)
                     /locus_tag="IW656_002771"
     CDS             complement(607..1164)
                     /locus_tag="IW656_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838437.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1343..1954)
                     /locus_tag="IW656_002772"
     CDS             complement(1343..1954)
                     /locus_tag="IW656_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838438.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2067..2324)
                     /locus_tag="IW656_002773"
     CDS             complement(2067..2324)
                     /locus_tag="IW656_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838439.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2921..3199)
                     /locus_tag="IW656_002774"
     CDS             complement(2921..3199)
                     /locus_tag="IW656_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838440.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3233..3484)
                     /locus_tag="IW656_002775"
     CDS             complement(3233..3484)
                     /locus_tag="IW656_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838441.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1199 a          810 c          526 g         1422 t
ORIGIN      
        1 tttcttggag ttaaacgctt ctgtcccagc ctccttattc cgaataaatg gtgtccaaaa
       61 gcttgcatct gccagattat ctcatttctt atgacaagct aagcaaatta tctccaccaa
      121 ataatttatt catatttttg acttgcgcat actaactaat tcttaaattt tcatacaaaa
      181 tttctcaatt tcccttaacg agtaatattt atgtacggtg attctcacta gtatcccaat
      241 cactttttaa gttatcccga tttcaattct acttcaaaaa tagttgcttt tatgtatgct
      301 gttagttgat tagatggtca tttgttgagc taggtcgttt attttaaaca agttgtttct
      361 ttagcttata cctatctcgt ctaccaaacg caagatggca aacacaaatg catacgcatc
      421 tgtatttgcc actttgattc gtataaagct aacaactatt aactcctctc agctaatagt
      481 tgctctcttt tttattcaga ttcatttttg ctgatcacag cctagtattt caatggtttt
      541 gaacactaaa ccaaattgtt ccattacaac attcactggc tgatgaaatg gattagtaga
      601 tttcgattat tcaaagcgat tttctattgg tggatcgtgt tcccaactaa taagacggat
      661 tccaaaccta tcgtacagat cttctaaacc acttataaat tttgcaccta tttcaggtgt
      721 tttattgcgt ggatattcta ttgttacgcc attaaatgga taccaattag gattaggctc
      781 tctttttttc tgtacatctc ccatagaata gttagcactt tctgggacat aatatattct
      841 tcgaccatcc tctaattcat ctaataagat atttcgtgat cgtctatcac ttgctaaatg
      901 tagacgccac gtgcgatacc atgccatgct acctgcatct gcagaaaatc catcaaataa
      961 tgcaacaatt gtatcttcta tcacaatcct ttccccatct acttcatatt ctaatctaaa
     1021 atcaaactcc cctcgggtaa tttctggtct tggtggctta ggacccaata ttattctact
     1081 taaaaccgca ttcgttccca gatgaaacac agctataaca acaataatga ttaatatttt
     1141 gtttctattt gataaatttc tcattattcg cacatcacct ctacattctc acatattagt
     1201 tcgtaatact cctttaatat caaaatatct actggtcgta ataatattcg ctatgaattc
     1261 ctactactcg tataagaaag gccaaatcat cagcttttac ttgctgatga tttggcttgt
     1321 ttctatttat cgtaattgtt ttctaatcac caaaattgtt aacaatcggt tcagatattt
     1381 cccagttgat taaggtaatt ccgtattgtg tcaaaacttc ttgcgcttct ccgaaattaa
     1441 gtacagttct tatgtatcca ctttctccta atataaagtc tacctctctt ttaaaacctt
     1501 cctcgagatt tctaagggct atatgtggtt ttaatgacat tgattgtaca ttttccccca
     1561 tataatattg cgcacttcct cgactaaaat agattcctag atcgtcccca aaaggtagat
     1621 cttctctacc actagctaat ctacttctcc atcttcgttc tcttttcata gaacctgcgc
     1681 tgaactcaca cccgttaaac tcagctatga cagtatcttc aattatgtta atctgtccat
     1741 ctagttcgta ttcaatccta aatggaaatt ctccataggt gatttccggt tcgggtattt
     1801 taggtgagat taatgcaaag aaaggaaata ttaagcttgg caacatcaaa aaaactcctc
     1861 ctatgatcac aattcccgat gtaatccaaa atataatttg tacaattctc attgatccat
     1921 tgtattccat tttattcccc ctattactat tcatatccgc ctatctatat cgatcttcca
     1981 atgcagagat aattgcttcc ataccttgat tttttactga gttacctatg cgagatatca
     2041 ttctccacta aggtgcatca tattgtctat ctaacatgat taattctcca ataggtgtat
     2101 actcctccaa aaacaacatt taatgcatct ccctcgacaa cataagctac catgtttgct
     2161 gtatatgtgt ttgagtctaa ttcataagca gataaattta ctgtcgctgg attaaatata
     2221 atcgcattcg tatttatagc tactgaattt gctgcttcag caccaccttt tgaatgtccg
     2281 atcattgtga tttcaatagt aggatttacg tggacagccc tcaccgttta ccctgaccat
     2341 tatagaaatc gcttactttt ttgaactacg caacaaagga tacaatgctc ctaacttcac
     2401 tgtctccatt tttgtccact aaggaatata aaaaataggc attaattcat ttaattcggt
     2461 cacaacacgt taccttttaa atcattatac tgtatctcat acattattat caataaacta
     2521 ttataaaaat gcaaaaaatg ctttatcaat ctattttgta attggtcata atatcattga
     2581 aatcataccc tgtatcgatt tttcctatga atctcgtcta tttgtgattg acgaaaaaga
     2641 ggcaagacag acactggaat ggtaatttta cccactattt tgctccaact atttgtaatc
     2701 gtacccactt ttgaaatacc gagatgatca ttttaattga tatgctgatt cccaagcaaa
     2761 accccaagaa attaaatgct ctatcgcatc tagcttcttg ggttttgctt atataaattc
     2821 caacatttaa attttttatg ctgttcggtc tcagaaatgc cgtttttgct tatatcacaa
     2881 tatcaagatg tcaaattatt cgtgttgccg atgactttga ttaatttttt tcaaattgtt
     2941 tattaagact aatgctgtct ttttgcagat accaatgttc gaacttttta acattatcat
     3001 atgatcctgc aagttttaac ttcttaccat tagatgttat caaagttata attaaccttg
     3061 cctttttccc attaggatct tttattttat gaattttctg caggtaatct ctcctaatgg
     3121 cagctatttt tacctcatca gtgctgataa accattgatc atccacatac atactgcctg
     3181 ttatgttgtg tttttccatt tcttccgaga acgaaaagtt taaggctcgt tcttacttcc
     3241 aaagttgaat gagtccatgg attggactta gtgtgagaat tgagaacaac aataagaatc
     3301 catatcctcc cgcaaacatc cctaaggcta tactgtctct ccatccataa gtaggattag
     3361 aagagaaaag agtcaccgtt tgtaataata tgaaaaggaa taatccagca caaacaccaa
     3421 agataacaac tccaaacatt gaatttttat acaatgacca tttatttata ggcttttctt
     3481 tcattttact tccccctact gttctgttca cacggaatga attcaaagtt cataagccct
     3541 cctctttgaa catatgaata cgacttgcta actatacttt aaaaaagctt gttttaagcg
     3601 gttataggcg agttgtaatc tctattcatc tcattagcaa ataacttgcc gaaaagtcta
     3661 ctgaaattaa agcagatagt ggaagaatta tttagccagg atagacacta tttgtgaaac
     3721 acatgtcttt acttttgaaa ttctatgatg aaaagccttg gaatacagta gctcttaatt
     3781 ggtaatactt tttccattat aaataacctc ttttcatttt atatttatcc cctctaaaaa
     3841 taatatctaa tctaatattt taaacttata tttccctcgc tgtgctaata gagcattact
     3901 aaataaatag aaactagaaa aaagcattac gcctgccaga ataaaccaat tatttgt
//
LOCUS       DACEJZ010000150         3927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEJZ010000150 DACEJZ010000000
VERSION     DACEJZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.160"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW656_002776"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW656_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0838442.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IW656_002777"
     CDS             complement(3250..3768)
                     /locus_tag="IW656_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0838443.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1271 a          642 c          591 g         1423 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaat
//
LOCUS       DACEJZ010000151         3809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEJZ010000151 DACEJZ010000000
VERSION     DACEJZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.162"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            93..2192
                     /locus_tag="IW656_002778"
     CDS             93..2192
                     /locus_tag="IW656_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0838444.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2388..3251
                     /locus_tag="IW656_002779"
     CDS             2388..3251
                     /locus_tag="IW656_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0838445.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3356..>3809
                     /locus_tag="IW656_002780"
     CDS             3356..>3809
                     /locus_tag="IW656_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0838446.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1523 a          636 c          772 g          878 t
ORIGIN      
        1 gtaaatccta aaaataagct attggcgtga cgtatagaat atgatagatt aaatggtagc
       61 gtaaacctag taaaggagcc gatcgctaag tgatgaaaaa atgtccaaat tgcggatcga
      121 aaaaaatgga gcagaatact atctgtccag actgcggtca tcaaataact gcagaagatc
      181 agcaaataaa agaatcaatt gaagaaacaa atacagatgc agctatgcag gataaatcaa
      241 ctgaagatgc agtctttcct gatacagaat taaacgatcc aatcgaatgg tcggaattga
      301 aagatttacc tctagaatct gtaatggaat tattcgaaaa agcagaacca gaagacgaag
      361 aaccacctgc tcacgatcaa gtaactaata tagagaaaaa tgaacagaag aaagcaactg
      421 ttcatgagac ttcggaagaa tcaggaaaga atcaagaaga aacgcttgaa tcaaaacaaa
      481 aaagaagagt agctgaactg aaagaaatcg tcgataatga ggaagaaaat tcgatactat
      541 ccgcttatat caaagcacat cgtgaagata caaaagaaga gcacgcgaaa gagctgctca
      601 aaatgatcag tgaaaaaatt gcacaggaaa acaaagtaga gccagaaaag gaacaagaaa
      661 ctgaaaaaga ctctgataga gaaaacgcca caggtaaaga aacagtaaaa ataaaagacg
      721 taaaaacgga tatcacagaa aaaactgaag tatttactga aaaacaatta gaaaaggaaa
      781 aaacgaataa actgccggaa gcagagaaaa aagaaatact cgaaaacaca gacaaaaagt
      841 caacagagcc cgatgaaata aatagtgaat ccgatgctga aaatcaatct gtaagcaaag
      901 agggtactga tcttgaagca aaaatagaaa aagaagagaa ccagcctaag ctgtttgaag
      961 cagaagcgaa atcaataaca atcgtgccag atactgaaaa aacacatcaa gaatcaaact
     1021 tagaaaaaac agatccagat aaagcgcaaa agaaaaaatc aaaaaaagta ccttatctcg
     1081 ttttagcagc agtacttcta ctcggtggag gaagctgggc ttattatgac catcatcaaa
     1141 aagtagaagc cgaagtagca gcagaagcaa aaagacagca acaaatggaa gaacttcatg
     1201 agaaacttgc tgccttttat acagatgatc accatcaatt tattcgtgca gatatgattc
     1261 atcaagattt atcaaaacta aaaaatgatt tatccagtct taagaatgaa aaaggatatt
     1321 cagagttaga aacaacttac caagatatcc aatctaagat ccgagatatc caaaaagtga
     1381 atgagtggtt catagcacct gccatcgtcg atgatcatct agcagatgac atcaagttga
     1441 aagcagataa agagatccaa gcaatcgaaa cggataacac agattttgga aaattgattc
     1501 aacaagcaca aacagaagct aaaaatcaat atgctgaatt gcaaacagct aaagaaaaaa
     1561 cagctatagt ctttaaagac ggaaaagtag tggattcagc aacacgcgac caatacaaga
     1621 cggcaaaaga agcagtagaa aaagtgaaaa atacagatct atctgctcag ttgaatgaac
     1681 aattgaaaca agtagataag atgctcactg ataaagagaa aaaagaagaa gaggaaaaga
     1741 aaaaagcaga agaagcgaaa aaagcagctg aagaacaagc aaaacaagcc gctcaagcag
     1801 cagctgcagc tcaagccgct caacaagctg cggtcgcaca agcagcacag caagaagcgg
     1861 catcctcgac cccttcctct tcaaactcag cgaatcagcc aatcaaatct gccagagcaa
     1921 gtgatattgc cgatgcatct aatccagcat ggacgtgggc accaggtgtc caaagcaaag
     1981 tgattgctac atgtatccaa cgtggatata tcgttgaagg aggttacaga ttagaaaaag
     2041 ccagaatcga aaatggggaa ggctattata atctatatgc cacttcaaca aaatctgcat
     2101 tgatgaaagg cattggagaa agtgctttgc cattttacat cgtaaccatc aactgtaaaa
     2161 ctggatggtt cggaggaaat gggagcaatt aattttaggc aaatgaaaga ggctgtgagc
     2221 ctgccgttta acaccgttta ttcgaaaaag gggagtatga cacaactgtt gtcatactcc
     2281 ttttttcacc tttccaaacc aaggctcagc tattcaagta gataacatta tgttatgata
     2341 gcaaagaaat gtattaagaa aacctttcac tatagaggga gaaaaaaatg aacgatttat
     2401 tagcaactat atttacaggt attattattg atgaaaatga aaaaaactac tttgtccaaa
     2461 aaaacggaca aacctttggc ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag
     2521 ttgaaggatt cggttaccaa aatcaaaaac atgaaaatcg gatcactaca caaattccta
     2581 aaagccgtat cggtcattac gcttttggaa tcgttactgc tacacgaaaa gatttaggtg
     2641 cgtttgtcga tatcggtctt ccagataaag actttgtcgt atctcttgat gaattgccga
     2701 caatgacaga gctatggcct aaaaaagggg atcgcttgat gatttccctt agagttgatg
     2761 caaaagaccg tatttggggc agtttagcag atgaaaaaat attcaaatct ttaagcaaat
     2821 atggaacaga agaaatgaaa aacaaagatg tgataggaac agcataccgc ttgaaattaa
     2881 caggtacgta cgttttaacg gaagatttct atcttgggtt tatccatcct tctgaacgat
     2941 ataatgaacc tcgtctaggc gagcagataa aaggtcgagt gattggtgtc cgaccagacg
     3001 gtgtactgaa tctttcattg aagccaagag catatgaagc tatatcagat gatgcacaaa
     3061 tgattttaac cattttagaa cgttctgcag atcagcaaat caactttaca gataaatcgg
     3121 atccagaaga aatcatgaaa gcttttggta tcagcaaagg agcatttaag cgcgcattag
     3181 gtaatctttt gaaacaagga ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa
     3241 aatctaattg agaatccctt gcatgttttt cttaactagc ttataataat tttaattaac
     3301 atagtgttat atcttttttt atttgtaatt attataaatt gttggggttg agtaaatggt
     3361 catgaatgct atttcggcga tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa
     3421 gctgacaccg caaagagaag ctactctgct tgtgcttttg gaaaatgaga aagagcattt
     3481 atctgcagaa gaaatcttct ttttagtgaa aaaaaagagt ccggaaattg gacttgctac
     3541 tgtttatcga acccttgaaa tcttgacaga attgaaaatt gtcgataaag taagttttaa
     3601 tgatggatta gcccgttatg atttgagaaa agaaggcgcc aaacatttcc atcatcattt
     3661 actatgcttg aactgcggca cgatcgagga agtagaagag gatcttttac ttgaagtgga
     3721 gcaagtagta gaagaacgtt atcatttttt agtaaaagat catcggctaa cgttccatgg
     3781 aatctgtcag gaatgctatg aaaaagaaa
//
LOCUS       DACEJZ010000152         3800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEJZ010000152 DACEJZ010000000
VERSION     DACEJZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.163"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..637)
                     /locus_tag="IW656_002781"
     CDS             complement(23..637)
                     /locus_tag="IW656_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838447.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="IW656_002782"
     CDS             951..1373
                     /locus_tag="IW656_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838448.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="IW656_002783"
     CDS             1383..1586
                     /locus_tag="IW656_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838449.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="IW656_002784"
     CDS             1797..2381
                     /locus_tag="IW656_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838450.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..>3800
                     /locus_tag="IW656_002785"
     CDS             2550..>3800
                     /locus_tag="IW656_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0838451.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         1283 a          602 c          716 g         1199 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaagaaatt
     2641 tagatccatt aacaacagaa cttgttgtta tgagtcgatc tgataatact ggttcaggat
     2701 tgatattagc ttcttctcct gaagcaaaaa agcggtatgg tattacaaat gtgagtagac
     2761 cacgtgactt accacaacca tttcctaaaa cactacacgt tgttccacca cgtatgaacc
     2821 tatatatcaa gcgaaatatg caggtaaata atattttcag aagatatgtg gctgatgaag
     2881 atctactgat ttactcgatc gatgaatcga ttttaaaagt gacaaaatca ctgaatcttt
     2941 ttacgactga agaaacacga agtcagcgaa ggaagaagct cgctcaaatg atccaagaac
     3001 gtattaaaga agagttaggt ttgattgcta cggtaggcgt cggcgataat ccccttttgg
     3061 ctaaattagc cttagacaat gaagcaaagc ataatgaagg atttattgcg gaatggacgt
     3121 acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat gacggacttt tggggaattg
     3181 gttcgcgaat gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga gaccttgcta
     3241 actggaatcc ttatacgatt aagaatagat taggtgtgat tggattacag ctgtacttcc
     3301 atgctaatgg gatcgatcga acagatatag caatcccacc agaaccgaca aaggaaaaat
     3361 catatggaaa ttcacaagtg ttaccacgag attatactag aagaaatgaa atcgagctgg
     3421 tggtaaaaga aatggcggaa caagtagcga tacgcattag gcagcataac tgtaaaacag
     3481 gttgtgtcca tttacacatt ggcacgtcga tcttagaaac gagaccaggg ttttcacacc
     3541 agatgaagat ccctatcaca gataatacaa aggaattgca gaattattgt cttttcttgt
     3601 ttgataagta ttatgaagga caagaagtaa ggcatgttgg aataacctat agcaagcttg
     3661 tgtacaccga ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa attgacgaag
     3721 aaaatttaga taaaattatt gacaagattc ggcaaaaata tggttttacg tcgattgtac
     3781 atgcttcttc aatgttagaa
//
LOCUS       DACEJZ010000153         3769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEJZ010000153 DACEJZ010000000
VERSION     DACEJZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.164"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(65..138)
                     /locus_tag="IW656_002786"
     tRNA            complement(65..138)
                     /locus_tag="IW656_002786"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Ile,seq:cat)
     gene            325..648
                     /locus_tag="IW656_002787"
     CDS             325..648
                     /locus_tag="IW656_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838452.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            812..1066
                     /locus_tag="IW656_002788"
     CDS             812..1066
                     /locus_tag="IW656_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838453.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            1136..1381
                     /locus_tag="IW656_002789"
     CDS             1136..1381
                     /locus_tag="IW656_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838454.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1478..1822
                     /locus_tag="IW656_002790"
     CDS             1478..1822
                     /locus_tag="IW656_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838455.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1883..2446)
                     /locus_tag="IW656_002791"
     CDS             complement(1883..2446)
                     /locus_tag="IW656_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838456.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(3014..3214)
                     /locus_tag="IW656_002792"
     CDS             complement(3014..3214)
                     /locus_tag="IW656_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0838457.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1134 a          659 c          590 g         1386 t
ORIGIN      
        1 agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
       61 cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt ctgctctaac
      121 caactgagct aaaggtacat cataaataaa atttattttt gttacttttc aactatagca
      181 cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt attcttattt
      241 cttttacttg tgtcataact attttgttac tttaattttt atttgcttat tttagaaaaa
      301 atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt tattgcgatt
      361 ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca aacctcagga
      421 attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg
      481 ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt taaatatgtt
      541 tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt cctcttttta
      601 tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat aagacacatt
      661 cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt tttcattgat
      721 ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac atgttatctt
      781 ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag ttttgttttg
      841 ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga acaaagaacg
      901 gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag tttttaatta
      961 caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg ttttgtcttt
     1021 attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac tattgattgc
     1081 atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg attaaatgag
     1141 tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct taagaactgg
     1201 ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat tcaaatatcg
     1261 taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata tcgctgactt
     1321 ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca aactgaagtg
     1381 attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac aatctatata
     1441 taaaggagac atttctatgt gattatggca tgaaaaattg atttcaaagc ttcctcgtca
     1501 acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct ggggaaagaa
     1561 acatgctact gtggattatg tttttcacta ttctccttat agattattcc aatatcatca
     1621 gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc
     1681 tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag agcgcaacgt
     1741 gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa atttggagca
     1801 aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc ttacttcatt
     1861 aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg ttatcttcat
     1921 cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact tccctagaca
     1981 taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg
     2041 cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt cttctacacc
     2101 ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc atgcatcgtc
     2161 gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct gtcgtggctt
     2221 cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct gccattgcta
     2281 gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc tgaaaataga
     2341 ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt tctaaatatt
     2401 ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt atttttaaaa
     2461 ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta
     2521 cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg
     2581 aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa
     2641 acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag
     2701 atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa
     2761 tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga
     2821 catctcgatt catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc
     2881 ctctaccata aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt
     2941 caacgaccaa tgaatttttt cgtacaaaaa aaccgtcttt tctttcgaaa agacggttaa
     3001 aagtcaattt ttattaagct ttaacaatgt ttgttgcttg agggccacgt tgaccatctt
     3061 caacatcgta agacactgct tggccttctt ctaaagtttt gaagccgtca ccttggatag
     3121 ctgaaaaatg tgcaaataca tcatttccat cttcaccagt gataaagcca aaacctttgt
     3181 ctgcgttgaa ccattttact gtaccgttat tcataaataa attcctcctg atacgtatca
     3241 atgtacgttt tttcgttgca ataaatattt gataggcaaa aggagtttta tctatgtaga
     3301 tatatcgctc aaattacgtt tcaaaataga ttactcattt aatatatcat gctttttcta
     3361 ataacacaag gataatgcaa tgaaatataa aattcattgt cttctattgt cttcgaatcg
     3421 aagaaaaagt tgaataaccg cgattgattc actttttgtt ttcttgttta tttgctttta
     3481 tctgcaactt ttgactttaa aatttcttaa ctatgaaaac ctttcaattg gtggtatact
     3541 tataaatgga atttcgttat caacgcgttc gttgcctttc agcaagtatt taaagaaagg
     3601 agaatggcta tgacattcta taacaaagat atacaatatc gagtaacctt ggatacagat
     3661 ttgaatctat ttgtcgtttt tgacaaagaa aataccaatc atgtagcgac aggtgttacg
     3721 atcgaacaag cagtgcaaga attaaaaaaa tctgcataag gagtggaaa
//
LOCUS       DACEJZ010000154         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEJZ010000154 DACEJZ010000000
VERSION     DACEJZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.165"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(82..1029)
                     /gene="arcC"
                     /locus_tag="IW656_002793"
     CDS             complement(82..1029)
                     /gene="arcC"
                     /locus_tag="IW656_002793"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0838458.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(1144..2163)
                     /gene="argF"
                     /locus_tag="IW656_002794"
     CDS             complement(1144..2163)
                     /gene="argF"
                     /locus_tag="IW656_002794"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0838459.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(2254..3483)
                     /gene="arcA"
                     /locus_tag="IW656_002795"
     CDS             complement(2254..3483)
                     /gene="arcA"
                     /locus_tag="IW656_002795"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0838460.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1001 a          776 c          645 g         1290 t
ORIGIN      
        1 aaaaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt
       61 aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa
      121 aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac
      181 aaatgcaata gctgcttcta ctttcggaag catgcttcct ggagcgaatt gcttttcatc
      241 aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg
      301 gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat
      361 ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc
      421 agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg
      481 atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac
      541 tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat
      601 ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa
      661 tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat
      721 ccctttttcc ttcagtactt cgcccatggc gttttgtaac cagtaaccga tagatccttc
      781 tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg
      841 ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc
      901 tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc
      961 ttttgcactt gcatccgtag acaagatggc attgccccct aaagcgacaa ctacttttcg
     1021 tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag
     1081 ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta
     1141 tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga
     1201 gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc
     1261 tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt
     1321 tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt
     1381 ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt
     1441 atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc
     1501 tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc
     1561 cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc
     1621 tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca
     1681 gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc
     1741 gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa
     1801 ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc cgtcaaacat
     1861 acgtcctagt actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt
     1921 tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg
     1981 agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg
     2041 tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa
     2101 ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt
     2161 catattaaac attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg
     2221 cagctgacag taagactgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg
     2281 ttggctcatg cagcgtggac caccacgacc tcgtgacaat tcacttgaat tgatttctaa
     2341 cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac
     2401 tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc
     2461 aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc
     2521 tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc
     2581 gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt
     2641 atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt tgttcggaat
     2701 tttgattgcc aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt
     2761 ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa
     2821 gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat
     2881 tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg
     2941 acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg
     3001 gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc
     3061 tgacagatca tatagactgc tagaacgtac ttcgatatct tttgtacgaa ccccagccat
     3121 gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct
     3181 tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac
     3241 attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc
     3301 attttgcaat gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat
     3361 atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg
     3421 ccgtttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc
     3481 catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca
     3541 ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa
     3601 ataattaaac gtttttctat ttttaagcgt aatttattca ttttttattc tgtatacact
     3661 ttcaaaaaaa tatttattca ttgaaaaaat ttttttgaat aaattaaaaa aa
//
LOCUS       DACEJZ010000155         3676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEJZ010000155 DACEJZ010000000
VERSION     DACEJZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.166"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..280)
                     /locus_tag="IW656_002796"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IW656_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IW656_002797"
     CDS             complement(546..740)
                     /locus_tag="IW656_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838461.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IW656_002798"
     CDS             2092..3132
                     /locus_tag="IW656_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0838462.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         1354 a          510 c          536 g         1276 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaa
//
LOCUS       DACEJZ010000156         3616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEJZ010000156 DACEJZ010000000
VERSION     DACEJZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.167"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..155)
                     /locus_tag="IW656_002799"
     CDS             complement(<1..155)
                     /locus_tag="IW656_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0838463.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTD"
     gene            849..1235
                     /locus_tag="IW656_002800"
     CDS             849..1235
                     /locus_tag="IW656_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0838464.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1329..1520
                     /locus_tag="IW656_002801"
     CDS             1329..1520
                     /locus_tag="IW656_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0838465.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2034..2366
                     /locus_tag="IW656_002802"
     CDS             2034..2366
                     /locus_tag="IW656_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0838466.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2662..2817
                     /locus_tag="IW656_002803"
     CDS             2662..2817
                     /locus_tag="IW656_002803"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0838467.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3093..3616
                     /locus_tag="IW656_002804"
                     /pseudo
     CDS             3093..3616
                     /locus_tag="IW656_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1289 a          615 c          575 g         1137 t
ORIGIN      
        1 ttatcagtta gtaacttttt ataatcataa aaaaaataag tagttctatc tgagcgatac
       61 tcttttataa tactgaattc ggaattaaaa accacgatag gtaactttaa acaaacatga
      121 agagacttta ataagacttc tttatctgaa aacacttttg tttctctctt tcctttctac
      181 atcattaaat agtattttat acgttatcta aaatactatt cctctgtcaa aataagctaa
      241 cttacttcat tataaaatat ccacttatcg ctttcatgta tattccaccg taaaaagtac
      301 attttatcac cgagaactca tcatttttca tcatgatgaa tactatttac atatatctag
      361 ttcaaagaca ataattatta tgatccgtta ttatctaagc actatacaga taataggaat
      421 ttttcgtttc taaatattcc aatttgggaa tccaataaaa attttcgttc aaatacgtgt
      481 ctattcgaac tcttttctag ttgttaatta tgaaggggga agaaataaaa aatacaatct
      541 atacacgaat aagaatatta tccaaaagga ggttttagaa aagatttatt atacgtaaaa
      601 agctcaagac attttcttag ccataaggac atgtttgata tttaagaaga tatatgaatt
      661 ttcttttcta agtataaaga taactatcca ttatcaacta aaaaaacatt tttgaaaata
      721 aacatcagca gtcctatcaa tgcatgtgag cttatagaag ttattcgcag cgcagcataa
      781 ttgcattagc ttcttttcgt gttttttgcc tcttttccct ttaaatctta atggaggagg
      841 tgtgcaatat gtcagataac gacgaacgcg taaatgccca gttgattcga ttctttgaaa
      901 aggtcatttt gaacaccgct aatacttatt ataaaaaaca agaagaaatt caaaaacatg
      961 aacaacttga tcaactccca ccatatttgt ttactaaaga tactataaaa tttaaacaac
     1021 cgattaatgt tttaaatgtg actctccttg ttgaagatat aaaattagca aaaatccttc
     1081 ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa atttatcttt gataaaactg
     1141 ataaagaaat tggaaaggtt ctggggatta caagacaggg tgcgacaaat ttaaaacatc
     1201 gactttacaa aaaaattcaa aaaatgctgg tttaatatct gcataataac taattttgta
     1261 tgcttctttg ctcgttctcc tccttcccaa agaaatagat tataagtcca tgggaagaag
     1321 gtagatttat gaattcttta aaagaagatt tcattttagc aaaggctgga aatgaagaag
     1381 ctgttgaggc aattctcaaa agatttagct ctttgattca taagcaatcc tggagaactg
     1441 gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga
     1501 aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt
     1561 tcaaatttcg aacaataaat ttgaaactat cttctttata attataaagt atcgatttta
     1621 cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatc aacgtttttt
     1681 aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa ttactttatt
     1741 tacgtatgaa gtgggcagga ggtcgaatcc agaactgggc tatggtactt caacgaggac
     1801 ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta gatttattca
     1861 actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat tttggttgtt
     1921 aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac taagttgggc
     1981 tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact ctattgagaa
     2041 aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag aaaaaagtaa
     2101 ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc cgatgggttt
     2161 cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga ttacctgata
     2221 aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa gacgagtgtt
     2281 ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt acgatgaagg
     2341 aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt tatgcacaag
     2401 aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag aaatggtaaa
     2461 aaaatgttga atcgataatt tgaacagaac caaattgatg ctgtttgggt gatggatatt
     2521 acttacattc catgtaatga cggtcgcctc tatttgtcca cctatattga cttagcaact
     2581 cgtattctac gttactatat ggtagattca cacatgaaaa aagagattgt cgtccgtcca
     2641 ttacaaattt attaaggaga attgcctacg gtaattcatt ccgatagagg tagccagcat
     2701 cggtctttta tttatcaaga gctgttgaac gaccaccaca tcacacattc agtgagccaa
     2761 actgggactc ctgttgacaa tgcagtaatt gaagcgtttc atcgatcaat caaatgagaa
     2821 ttaatcgact ccaacaaata taaaagtaaa atagagatga aggtgttaat ccaagattat
     2881 ttagaagact actacccaaa caaaagaatc catacaaaat ttatgatgac tccttgacaa
     2941 tttgaagcga agaaaataag ggatctataa tatctagtgt tggtgacgcc ttctgttgcc
     3001 aatacttttt cttttttcta ataaacgcaa acagacacct taaaatcctt tagaataaag
     3061 tcgacgaaaa ccattcaaag gagtgtttca agatgtccta ttcagagttt accaaagagt
     3121 tactagatat tctagaccta aatcttatct ttcatgaaga tgcctttcgc aaagagcgaa
     3181 tcaatgacga aacatgtttt gtttttgatg gcacattgac ctatcaaccg gaggaatgct
     3241 ttcatcgcca ctatcaaaat gaacaaacga tcattaaata gggctggaaa aaagtatcca
     3301 tcttattgaa tgatgtcagt aactacaaga ccattctgcg gattaacaaa cagcgcttca
     3361 aatgtaaaca ctgtggaaag acttttttag cagaagattc agttagtgat cgtcgctgct
     3421 ccattgctcg cagagtcaaa caagccattc ttgagctatt aagcgaacct ctttctatgt
     3481 ctttgatcgc acgtatgaag cacatttctc cgaccactgt cattcgaata cttcgcagcc
     3541 ttcggcctaa aaccatttct ttgagccagc tgttaccaga agtggtgtgt ttcgatgaat
     3601 tcaaatcagt gaaaaa
//
LOCUS       DACEJZ010000157         3526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEJZ010000157 DACEJZ010000000
VERSION     DACEJZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.169"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..530)
                     /locus_tag="IW656_002805"
     CDS             complement(27..530)
                     /locus_tag="IW656_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0838468.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IW656_002806"
     CDS             complement(653..1417)
                     /locus_tag="IW656_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0838469.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1524..1799
                     /locus_tag="IW656_002807"
     CDS             1524..1799
                     /locus_tag="IW656_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0838470.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2515..3465
                     /gene="yidC"
                     /locus_tag="IW656_002808"
     CDS             2515..3465
                     /gene="yidC"
                     /locus_tag="IW656_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0838471.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         1069 a          721 c          668 g         1068 t
ORIGIN      
        1 atatggtcta ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg
      121 tccacgaatg tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttgtacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt
      721 tcattgggat atatacgttc tgatcagccg aagacaagat ttcctgactt actccctgac
      781 cttcgtttcc catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg
      841 cttcttcatt taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca
      901 cttctgccaa atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca
      961 gcacctttgt actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa
     1021 acgcatctgc cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta
     1081 aaagccagcc tcctgaaaaa tctggcgtat cttcagatgt taacgtcatc actgctaaga
     1141 tcccttgcgg tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag
     1201 tagaaatctc tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcgatcaga tggaaccctt
     1321 ccaaaagata ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttact gattggatct ctttcacatt cctcacctca actgattata
     1441 tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa
     1501 aacaggaaag aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc
     1561 agggcgtggg atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa
     1621 cggtaaaaaa cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca
     1681 tccaaaaatt tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg
     1741 tggatgtcca agaagatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag
     1801 gttgtttttg aagcgctttg cctcgagtat taaggagaaa cattttccaa atagcttctc
     1861 atattttgaa atgtaatgac ttaataccga gaggcagctg ataaaacacc gtcaataagg
     1921 ttgtgaaaaa gctggtctgc atcaagcatt aaggactggt tgtgaaaaat agctctccat
     1981 atttttcaac aggctggact taatgccgta gatgcaagct tttgaacacc gtcgataagg
     2041 ttgcgaaaaa gctgatctgc atcaagcatt aaggactagt tgtgaaaaag tcgcttagtt
     2101 ttgagtatta aggaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt
     2161 aataccaaga aactgacttt tgaacatcgt cgataaggtt gtttttgagg cactttgcct
     2221 caagtattaa agacaggtag taaaaaagct gatctataac ttgcaataaa tttgcttaac
     2281 ccatattttc cagacagaac aaaaaagttt ttagtttttt aaataagaaa gaaacacttg
     2341 gaatcactct tttctttcta agtatttctt tcttgatttt ttgtcttttt tcaaaatttt
     2401 tcgctacgaa aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg
     2461 cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat
     2521 aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc
     2581 tgtgtgcgga tggattcaaa cggaaaccct gacacttcag gaatcgttta tcgagtactc
     2641 gtccatccta tgggccaagc gattacatat ctagttgaga atttcaattg gtcttacggc
     2701 tgggcagtta ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact aggtatcagt
     2761 caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa
     2821 gcagcacaac aaaagctaaa agctgcaaca acacgtgaag aacaaatggc agcacaagca
     2881 gaaatgcagc aagtatatcg cgaaaatgga ttgagtatga ccggcggaat cggctgtctg
     2941 cctctattga tccaaatgcc gatcttctct gccttgtatt ttacggctcg ctacacagaa
     3001 ggtatccgtg aatcatcttt ttacggcatc gatctaggct caccaagtat ggtgcttgtg
     3061 gccatcgcag gggtcgctta tctgcttcaa ggatatattt caacaatcgg tattcctgaa
     3121 gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg
     3181 tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt
     3241 ctgcaaacat tcatcacaaa cgttataatg aaaccgcgtt tgaaagcaca agtggcagaa
     3301 gagttgaaac aaaacccgcc aaaacaagtc gttactccaa gaaaagacgt gacagaatca
     3361 gcaaagccag ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca
     3421 aacaacaaag gccgcaacgc aggaaagcag cagaagcgga aataaaaata tatgacaaaa
     3481 aactgtagac aaataaaaaa atttctttgt tctacagttt ttttgt
//
LOCUS       DACEJZ010000158         3369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEJZ010000158 DACEJZ010000000
VERSION     DACEJZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.170"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW656_002809"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW656_002809"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0838472.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW656_002810"
     CDS             complement(2260..2511)
                     /locus_tag="IW656_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0838473.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW656_002811"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW656_002811"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0838474.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          739 c          626 g         1084 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaa
//
LOCUS       DACEJZ010000159         3209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEJZ010000159 DACEJZ010000000
VERSION     DACEJZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.171"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..976
                     /locus_tag="IW656_002812"
     CDS             116..976
                     /locus_tag="IW656_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAQ0838475.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IW656_002813"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IW656_002813"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ0838476.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IW656_002814"
     CDS             1830..2678
                     /locus_tag="IW656_002814"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ0838477.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IW656_002815"
     CDS             2688..3083
                     /locus_tag="IW656_002815"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ0838478.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1246 a          327 c          642 g          994 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tatcaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttat
//
LOCUS       DACEJZ010000160         2922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEJZ010000160 DACEJZ010000000
VERSION     DACEJZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.173"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            158..2347
                     /locus_tag="IW656_002816"
     CDS             158..2347
                     /locus_tag="IW656_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0838479.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2344..>2922
                     /locus_tag="IW656_002817"
     CDS             2344..>2922
                     /locus_tag="IW656_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0838480.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVN"
BASE COUNT         1110 a          392 c          478 g          942 t
ORIGIN      
        1 ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca tttacacaaa
       61 attattgacg ctcccttata atgagaactt ttactaacat aatttatgat aaacttacta
      121 tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta ctcttcaaat
      181 tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga caaatactat
      241 tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa tcttattaga
      301 tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc atggaattca
      361 aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa tcagcgaaga
      421 agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc aaacaatctt
      481 agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat ttattagacc
      541 taacaaagct attagaaagc aattatttga tatttccact gaattagact ctcatcaatt
      601 taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca catctaaatc
      661 aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac tcaatgacaa
      721 tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa ttgacatgtc
      781 agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg ttcttagaga
      841 aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat caaaaatatc
      901 atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga ctattaccag
      961 tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata gcataattgg
     1021 ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata tgggttcttt
     1081 agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac tagggttagg
     1141 acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt gtcgttcaag
     1201 agaactactt aatattatgt tgattgaaga accagaagct catattcatc atcatattca
     1261 aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc tcatgaccac
     1321 acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat taaaatcgtt
     1381 taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg ctaaagaaaa
     1441 gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag atgttaaaag
     1501 aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg aagaaatact
     1561 tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg gaataggttt
     1621 gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg atgatagaat
     1681 acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat cagaacatcg
     1741 attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat tatcctccct
     1801 ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg aaattgagtt
     1861 tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag atgacgcatt
     1921 caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac aatatttaga
     1981 aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt ttgcaattga
     2041 gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta atgctattgc
     2101 ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac tccatacaat
     2161 cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa aaacaagtga
     2221 tacagctgat ttgaatatag caataatttc tttattagag tactttggta aagatgagga
     2281 tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg ctgaggtttc
     2341 aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca aaaagatgtt
     2401 gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa aacaagggta
     2461 cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa aagaataatt
     2521 gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag tttacttagt
     2581 atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct tgaatttatt
     2641 atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat tattgatgat
     2701 tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa tctcaaaacg
     2761 tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa tgaagttcag
     2821 tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga aattgatttt
     2881 gatatgattt tactagtagc gtataaaatt ttaactgtaa ac
//
LOCUS       DACEJZ010000161         2919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEJZ010000161 DACEJZ010000000
VERSION     DACEJZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.174"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..1173
                     /locus_tag="IW656_002818"
     CDS             205..1173
                     /locus_tag="IW656_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0838481.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="IW656_002819"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="IW656_002819"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0838482.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="IW656_002820"
     CDS             2203..2811
                     /locus_tag="IW656_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0838483.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          709 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaag
//
LOCUS       DACEJZ010000162         2861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEJZ010000162 DACEJZ010000000
VERSION     DACEJZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.175"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..2376
                     /locus_tag="IW656_002821"
     CDS             4..2376
                     /locus_tag="IW656_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838484.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..>2861
                     /locus_tag="IW656_002822"
     CDS             2437..>2861
                     /locus_tag="IW656_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838485.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          455 c          620 g          642 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg c
//
LOCUS       DACEJZ010000163         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEJZ010000163 DACEJZ010000000
VERSION     DACEJZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.176"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW656_002823"
     CDS             <1..1023
                     /locus_tag="IW656_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0838486.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="IW656_002824"
     CDS             1157..2500
                     /locus_tag="IW656_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0838487.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..>2784
                     /locus_tag="IW656_002825"
     CDS             2612..>2784
                     /locus_tag="IW656_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0838488.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1111 a          361 c          517 g          795 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctgggaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac tttt
//
LOCUS       DACEJZ010000164         2781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEJZ010000164 DACEJZ010000000
VERSION     DACEJZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.177"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            701..1513
                     /locus_tag="IW656_002826"
     CDS             701..1513
                     /locus_tag="IW656_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838489.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          977 a          372 c          423 g         1009 t
ORIGIN      
        1 tttcagtgta aaatgtactt gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta
       61 aaaacattgt accgtaaaag gactgttaca tggccttttt acttttacac aattataagg
      121 actttatcga aaaaaagcag ttatttgtta aaatcattga tattattaat aataagtatt
      181 ttttgaaagg ggaaatataa aaggtttttg aaaataaata aaaaatagta ttacttgttc
      241 tattgctagt tattgctagt ctttattctt ttttgttttt tagggatgta caaaagatct
      301 caaccacagg agacattgct tttcactttt ctagaataaa atgcttgtcc tcagtatttt
      361 ctggtccaat aaattttacc acatttaata gctatggcgg aggcgtgaat ttttttatcc
      421 atatctaaca tctttccagc cgtaatattt tactggacaa caaacaattt gattctaagt
      481 tatatactta ctatacgttg ggatactgaa tatctgtaca attctagtga gctatcaata
      541 tggacaaaag aatagatgca gccttcttat tcagctgttt gtatagcttt tatggattga
      601 tatatattag cgtactgcaa tttcagaatc aattgcgttg acgattgtac cagttgtctt
      661 gtattacaca tatactcttc tttatgaaag aaaaaactgc gtgattcttt tagctgtcag
      721 tatgagtttg ctcatctata cccatattct ctccaccttt atgagtgtga ttgttattgg
      781 cttcttgttt atcggctctc ttttctctaa gcggaaagag agaaaagaaa tgtcgaatct
      841 gatatcaaat atgataaaag ctgttggttg gaccattctt ttaacagctt acgtttggta
      901 tccgatgtta caacaagtcc tacatcaaaa aattaatcgt ccttttcgca caaaacttca
      961 agaaagggca ttaaacattt ctgatagttt aatcggtgca gtaaataatg atttagttac
     1021 atttacaatg ggaacaattg gtgtggtttc atttattctt ccacttttct ttaaaaaaaa
     1081 atttgccaat aaagaaaaga ttattaattt ttgtgcagtg accacttggt tcttatcaac
     1141 gaatcttttt ccatgggaat ttttacaaaa aacacctatt caaatcattc agtttccttg
     1201 gagaatcttg ggttttcaag tgctttttgg ttcattgatc ttaattatag tgtttttaaa
     1261 gtggaaaaca agcaacaaaa aaagtatgtg tagccttgta ggaatagtcc ttcttatttt
     1321 tacagtgact gtagcaacag aagcgaatta ttcgcagaaa attcaatcgt ataaaggtcg
     1381 tttaattatg accaaaaaag atgttacact ttatacaacc tcgagaactg gaggtcttta
     1441 tgactatgca ccattagatg cattgaaata taaagatcat ttaaagaaac atgaagtaaa
     1501 agtctatgat tgatgggaga atgcatctca taaagcacat aattctaaaa taatctatac
     1561 agtaggagat gcgaatgagc aaaatatcac gttacctgta tttaattact gggaacagtt
     1621 atgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
     1681 ggtacgggaa aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
     1741 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
     1801 tgcggttaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
     1861 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac ggtgaattta
     1921 ttccgaaaag tgaaaagaac aaatataaaa tttaataaaa atatagaact aaataggaaa
     1981 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaatttat
     2041 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
     2101 taggtctatt taactcatca gacattctat gctcattttc tccaaatttg tttcaactct
     2161 ttcgtactta attttgcgta tttgaaaatt cttcttttag agtactttta ttacgatact
     2221 agtatgatat atatctatag caaacagtta aaatcttcct aaaataagcg aatagcattc
     2281 ctaaaaccta taaaattttt agatgaagcg acaagtggac aattaataaa ctacttgaac
     2341 atgaaattat ggatcacctt tcaaatttag aaaatggaat tcttatactt attgcgcaac
     2401 atttattgat tgcaaaatta tgtgacaaaa agaactagta gaagaaggaa taatggcttg
     2461 ataataaagt actacctatt aaaggtagta ctttatttaa tttagtataa attaacatac
     2521 tactatattt taagtttcat attatataaa ttaataatat actaccattt tttggtagta
     2581 taatttaaag gtaataagtt attcttttat tactatgttt gggttttata gcaaggaaag
     2641 tttaataaga gtttggacct agcaaacaat attaatgagg tcattaaatc ttattatctt
     2701 tagtgatata acgaaatatc tacacagaca aaattatgat cttgcgtaaa gttataaaat
     2761 attaataaaa atattttatt a
//
LOCUS       DACEJZ010000165         2750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEJZ010000165 DACEJZ010000000
VERSION     DACEJZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.178"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..941
                     /locus_tag="IW656_002827"
     CDS             <1..941
                     /locus_tag="IW656_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ0838490.1"
                     /translation="EFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYIEDA
                     REKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADYIQE
                     LIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAE
                     RYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDSVEP
                     WNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGICKS
                     KFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1238..1786)
                     /locus_tag="IW656_002828"
     CDS             complement(1238..1786)
                     /locus_tag="IW656_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838491.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1787..2644)
                     /locus_tag="IW656_002829"
     CDS             complement(1787..2644)
                     /locus_tag="IW656_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0838492.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          802 a          513 c          408 g         1027 t
ORIGIN      
        1 tggaattctc tgactatgaa tcactccttg taaaaatttg tgaagactat tatctcgagg
       61 atatcaatat tagtgaaatt tctaaaaaat atgggatcac cagatataaa gctttaaaat
      121 atattgaaga tgcacgtgaa aaacaattag ttagtatttc aattaattct ccgtatgcta
      181 ggaattatga gctagaagaa atcttcaaaa attactttaa aacgaatatc tatatattaa
      241 aaagtagtga agacttatca caacacgatt tattattttc aaaatttgct gccgattaca
      301 ttcaagaatt aattaagaaa gccaatgtgg ttgcactatc ttggggagat accgtttaca
      361 aagtgattga tcagtttaaa atgggtgctc gtgaagaatt aatttttact caattcattg
      421 gcgaaatcgg aaaatataat tccttagctg gctccacacg attagttcaa aaagctgctg
      481 aacgttatga gtcttcttat ttgactctca gtattccttt atatgtcatc aatgaaactg
      541 cacgtgaact aatggctcta gaaccttctt tagctaatac cttagcaacc gcttcgcatg
      601 cagatatgtt aatttctgga attggcacct tatcctctat tgattctgtc gaaccctgga
      661 acaagcatcg taaaatccta tttccaagca tgacggatac tgttggattt ctttacggaa
      721 gacccttcaa tatcaatggt gaattcatca aagcatctga ggataagact tttggtcttt
      781 ctttaaaaga aattttcgat attccaaaac gaattggtat ttgtaaaagt aagttcaaag
      841 cggagagttg tttaggcgcc ttgaatggta atttttttac agatttattt ttggatgaaa
      901 aaactgcttg gaaaatactt tctttgataa aaaaagagtg agtcatgtaa aatcctccaa
      961 aaaatttaga tttttggtac tacttttttg gagggcagta caatgattca ctctttttat
     1021 tggatatcaa aatccctttt tttgagaagc ttgtcttgat attttttgaa cttttccaag
     1081 ccatactcca ttacttttca agatttaatt tctttgtatt cactgaataa tttccacgtt
     1141 ttttcgttaa tgttacttct tattaatttt attgtacttc tcatgcaata ggatcatata
     1201 tccttctaaa tcttttttat gctataaaaa gggggttcta tctaacttta ttattcattc
     1261 cccacccttt gcgcagtgag tcttcggtat cttgttttat ctgactggta gttcttatta
     1321 tttctccacg gtcatctaat tctggaacaa ttggatattt ttctctgaac aagtctagtt
     1381 ctttcaccac gtcattaaac aaatcttgct tatcttcttg cgtaagattc tgtttaatat
     1441 actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca tacttttcta
     1501 ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc ttggtggaat
     1561 aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct ctactctgtc
     1621 tttcaacatt ggaggaagtt tttatttctt gaacttggtg cacttctaat tgcttatttt
     1681 cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt attttggcga
     1741 gtcctttgtt gttcccaaaa atatcaagtc ctttgatgtc agccaattac tccacctccg
     1801 cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat tcgtggcttt
     1861 tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata cctgatccgt
     1921 cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt tccccataaa
     1981 tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc aacatgaaag
     2041 gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag tccatatact
     2101 caagatattg catcgcattt ctcatcgatt ttacttgggt ctgtaagacg acgattgccc
     2161 agtccgcaat gtacatcgcg gatttactat atacagaaat agaaggtggg acatctaaat
     2221 agatgacgtc aaactcatca aataatggtt ctagcttttc tttcatatat ttgaattggg
     2281 ataatgaatc attatattgg tctttgatcc aatcatcata catttcaaag gacatatctg
     2341 ctggaagtat atagaggtta ttgattactt tatagacagc ctctttaggt ggaagattgt
     2401 ttatcaacac gtcagtcaca gtattttcgt aaacaatcgg ttcaccttct ccgcctcctt
     2461 cttcgttagt gaagttcata tgcgttaagt tcatcacatc agaagtattt ccttgcatat
     2521 ccaagtcaat tatcaaggtt ctttttcctt cattaactgc tgattcaaac cctaacattt
     2581 gcgcgcacgt acttttacct acgccacctt taaaattacc aattacatat ttcttcgcta
     2641 ccacgttcct acctcttttc ttttagcaat attcatttga tactattata acatatttat
     2701 ttttagttac taccaaaaaa aagatatata tgtactacct tataattaat
//
LOCUS       DACEJZ010000166         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEJZ010000166 DACEJZ010000000
VERSION     DACEJZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.179"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IW656_002830"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IW656_002830"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0838493.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACEJZ010000167         2669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEJZ010000167 DACEJZ010000000
VERSION     DACEJZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.180"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..321
                     /locus_tag="IW656_002831"
     CDS             <1..321
                     /locus_tag="IW656_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0838494.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="IW656_002832"
     CDS             735..1553
                     /locus_tag="IW656_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0838495.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="IW656_002833"
     CDS             complement(1626..1985)
                     /locus_tag="IW656_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0838496.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          371 c          471 g          902 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattccccct taactataac tcaattctat ataaaaaata ttctattctc aaataattga
     2101 acttttcaaa tcgccaattt acttcggctt tatcagagct cattcatatc aagagcgatt
     2161 aacgctgtaa aaattaaacc gaagatctga taaattttct ctattttcta caatatttaa
     2221 aacgcttcct tagtctatta tatatctatt tctcttagga gtatgtttag atgtttgaat
     2281 aatagttgcc caactttaaa ggttattctt ataatatttt acttcatttt ccaatagaaa
     2341 attcaaagga ttactggttt ttctaaaata gtttagttta aatacataag tatcctccga
     2401 cactgtatta gttcataatc atctaaatac aaaattttca aataaaaata tttcaagctc
     2461 tatcgttgac gaatgtaaac gatacttata aagtaataga aattgattac atttgtaaac
     2521 ttaaaatggt taatttatct cacataaaac taagaataaa ataatgatta aaagtggtta
     2581 gtttttatac gatttagcct atcggtaaaa atgaattaga gagtgtaaaa tattttgtgt
     2641 aaatgaaaaa atccatacaa aaaaggaag
//
LOCUS       DACEJZ010000168         2651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEJZ010000168 DACEJZ010000000
VERSION     DACEJZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.181"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            751..2577
                     /gene="ltrA"
                     /locus_tag="IW656_002834"
     CDS             751..2577
                     /gene="ltrA"
                     /locus_tag="IW656_002834"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0838497.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          949 a          463 c          590 g          649 t
ORIGIN      
        1 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
       61 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
      121 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
      181 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
      241 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
      301 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
      361 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
      421 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
      481 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
      541 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
      601 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
      661 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
      721 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
      781 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
      841 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
      901 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
      961 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     1021 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     1081 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     1141 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     1201 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     1261 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     1321 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     1381 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     1441 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     1501 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     1561 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     1621 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     1681 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     1741 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     1801 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     1861 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     1921 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     1981 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     2041 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     2101 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     2161 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     2221 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     2281 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     2341 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     2401 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     2461 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     2521 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     2581 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     2641 agcgggggaa a
//
LOCUS       DACEJZ010000169         2617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEJZ010000169 DACEJZ010000000
VERSION     DACEJZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.182"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(492..1784)
                     /locus_tag="IW656_002835"
     CDS             complement(492..1784)
                     /locus_tag="IW656_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0838498.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2058..2318)
                     /locus_tag="IW656_002836"
     CDS             complement(2058..2318)
                     /locus_tag="IW656_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838499.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          886 a          483 c          398 g          850 t
ORIGIN      
        1 tatattataa attcacatga ttatcgaatt ttattaagtt agtttttctt gtaaatctct
       61 gttcccaatg aaactcgaga cacttatttt aaagaggaaa caatgtggta tttaaatagc
      121 cctcttgtat gggctactct gtgatactta ttagccatta aactatttta cccacatagc
      181 atatattttg aaatcgttca ttccgtaaat tcaactaaat atgcctaaaa aaataaaaaa
      241 tctaagatag aataagattt attcagtctt agatggattt atagcaactt tttcgtaaca
      301 actttaaaaa atacgaaaag aaagagctgt tttactctga ctacgttgct aaattgtcgt
      361 ttaatcattc atttaaacca aatctttttt tatagaattc gttttaatcc tcctgaatcc
      421 aaaaactatg aaaaccgtaa ttaaatagtt aagagttttt aaccaccata attctctcgg
      481 ctattttgaa atcagataaa ttttgatcgg acacatagtt gaataaacct accgttgtta
      541 cggaaggtaa atcgcatact tttcattcta gaggagaagg tctcgttgat cattaactga
      601 ttaagcacca tagcccagtt ttgaattcga ccacctgacc acttcgcgtg taattctttc
      661 gtgcgtaaat aaagtagttt taggagtgca ttctcattag agaacgctcc tttttttgtg
      721 acttttctga agctggagtg gacactttca accgcattgg tagtgtacat aatttttcga
      781 atggcactac cataatcaaa tagttgttca acatgtgcaa agttccgttt ccagacatct
      841 acagcaccag aataatgaga ccaccgattt tgaaaactgt caaaagcagc atgtgcagcg
      901 tttagagaag aagcaccgta gaactttttc atatctcggc agacttcctt atagtcctta
      961 cttggaatat agcgcaatgc atttcgaaca agatgaacaa tacagcgctg aacaattacc
     1021 gatggaaaga tcgcttttgc gccttcttcg aggccagaaa caccgtccat cgaaatgaaa
     1081 aagacatctt cgacaccacg tgctttcagt tcatcaaata cttgcatcca gcgattttta
     1141 gattctgttt gatttaacca taatcctaaa atctccttat ttcctttgag atcatagcca
     1201 agaatggtgt atacagcata ttctttggct tcataatttt ctcgtaaagt aacatacata
     1261 caatcaacga atagaaaggc ataacacttt gctagggggc gggcttgcca ttcttccaat
     1321 tcaggaagga cagcgtcagt gatatctgaa atcatttcat gggaaatatc aaagccataa
     1381 atagcttcga cggttgcggc aatatctcgt tgactcattc ctcgtgcata catggaaaga
     1441 accttccctt cgatgtcgga gacatctcgt tttctcttag gaattaactc tggttcaaag
     1501 gaagcttccc ggtctctagg aacatcaata gctacttcac caaaactggt tttaagcgtt
     1561 ttagttccat agccatttcg acggttatcg tgttccttag gctctttaga atgggcatca
     1621 taacctaaat gattattcaa ttctccttga agcatttttt caaaaagggg cccaaacaca
     1681 tctttcaaag catcttgcat gtcatcgaca gattcaggtt gataggcatt cagaatggat
     1741 tcagctaact tttcggcatc aggatttctt ttctttctag ccatcgtgta atcacctctt
     1801 gatcttattg tagaaaaaga aaaaccgcga agcaaccacc tgcctaggat taatggttac
     1861 ttacacggtt tatattacac tctcatcatt aaaaaagtat ctttcgatat tactaaacaa
     1921 tgaaattatc tatttgaaat ttaaatctga taatatattg agtgttccaa tgagggcttt
     1981 gatcctatta tccatatctt tttcagtttt aatattgata cacattataa gaaaaagaaa
     2041 cttttggtta atttatttca tcccagtgca aaatataaaa aagtaaaata gaccataggc
     2101 aatagattaa caattaatat aatacaagca aatattctat tcattgtatt tctgctttca
     2161 aaaagttcta atattgaaag tataattcca caaatgccta taataagtat gggaataaga
     2221 ttagaaaata acaccccgag ccaatctgtg aaaattacta tatttactaa tatccctatt
     2281 aacataccta tcatatttat taaatcaatt tttttcatta gatatttctc ctttattaaa
     2341 tctgaagtac ttatattaaa ataattctaa actatttacc tcaaaaagtg caataaatta
     2401 tttctgaaca ataaaatgat agtcaaaaca aaaaaatatt gttaatcgtt cagagccatc
     2461 cgagagtgta aaatattttg tgtaaatgaa aaaatccata caaaaaagga aggcgcttct
     2521 gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat gaatgatttt
     2581 actacagaaa ttgtgcaaac tctagtcact aaaggcg
//
LOCUS       DACEJZ010000170         2603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEJZ010000170 DACEJZ010000000
VERSION     DACEJZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.183"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..177
                     /locus_tag="IW656_002837"
     CDS             <1..177
                     /locus_tag="IW656_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838500.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
     gene            190..693
                     /locus_tag="IW656_002838"
     CDS             190..693
                     /locus_tag="IW656_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0838501.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="IW656_002839"
     CDS             753..1142
                     /locus_tag="IW656_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838502.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..>2603
                     /locus_tag="IW656_002840"
     CDS             1129..>2603
                     /locus_tag="IW656_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ0838503.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          773 a          430 c          600 g          800 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg gttcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tgg
//
LOCUS       DACEJZ010000171         2546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEJZ010000171 DACEJZ010000000
VERSION     DACEJZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.184"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            386..1966
                     /locus_tag="IW656_002841"
     CDS             386..1966
                     /locus_tag="IW656_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0838504.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2164..2388
                     /locus_tag="IW656_002842"
     CDS             2164..2388
                     /locus_tag="IW656_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ0838505.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          904 a          414 c          534 g          694 t
ORIGIN      
        1 agatttctct ttaccaacat catttgacaa agagcccttt tcttttagtc agctgacaaa
       61 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
      121 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
      181 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
      241 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
      301 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
      361 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
      421 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
      481 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
      541 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
      601 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
      661 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
      721 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
      781 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
      841 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
      901 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
      961 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     1021 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     1081 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     1141 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     1201 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     1261 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     1321 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     1381 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     1441 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     1501 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     1561 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     1621 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     1681 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     1741 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     1801 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     1861 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     1921 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     1981 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     2041 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     2101 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     2161 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaaaga agagatgtta
     2221 cgattatgtc gattattaga tgtaaaagac tgggacaaaa acgagcaaat tgcacaaaaa
     2281 gtacgatcaa tcatccataa agaggaaaaa gttaaaaata aagacacgcg tagaactgaa
     2341 aatgaaaaga gaaaagtaca aaagaaaaga gaaaaatcaa aaagctagaa agaaaaagag
     2401 gctgagacag aagcgtttaa ctccaagaaa taagctggaa ttcacaaaaa tttgaaaagc
     2461 aattttcgtg aatttcagct tatttccgaa ggagttgctt ctgctttcgc cgtttatacg
     2521 tgtttagagc gtgagacaaa agtgtt
//
LOCUS       DACEJZ010000172         2493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEJZ010000172 DACEJZ010000000
VERSION     DACEJZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.185"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..567
                     /locus_tag="IW656_002843"
     CDS             <1..567
                     /locus_tag="IW656_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0838506.1"
                     /translation="RINVCHTTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKI
                     KGRWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGS
                     AFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRG
                     IYKKNRRNGTLFSFSVSTEIKVLMGITA"
     gene            573..989
                     /locus_tag="IW656_002844"
     CDS             573..989
                     /locus_tag="IW656_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838507.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            1095..1358
                     /locus_tag="IW656_002845"
     CDS             1095..1358
                     /locus_tag="IW656_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0838508.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1358..1630
                     /locus_tag="IW656_002846"
     CDS             1358..1630
                     /locus_tag="IW656_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0838509.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1707..2036
                     /locus_tag="IW656_002847"
     CDS             1707..2036
                     /locus_tag="IW656_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0838510.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            2026..2313
                     /locus_tag="IW656_002848"
     CDS             2026..2313
                     /locus_tag="IW656_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0838511.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          918 a          340 c          486 g          749 t
ORIGIN      
        1 agaataaatg tttgtcatac tacgatttat cgttgggtgc aagagtacag caaagtcctc
       61 tattatcttt ggaagaagaa aaatagacaa tccttctatt catggaaaat ggacgaaacc
      121 tatatcaaaa ttaagggacg ttggcattat ctttatcgtg caattgatgc ggacggctta
      181 accttagata tctggttacg aaagaaacgg gatacgcaag cagcctatgc tttcttaaaa
      241 cgactccata aacagtttgg tgagccgaaa gcaattgtga ccgataaagc accttctctt
      301 ggctccgcct ttagaaagtt acagagtgtg ggtttatata ctaagacaga gcaccgaact
      361 gtgaagtatc ttaacaattt aatagaacaa gaccatcgac ctattaaacg acggaataaa
      421 ttttatcaaa gtctccgtac agcctcttcc acgattaagg gcatggagac cattcgagga
      481 atatataaaa agaaccgaag aaatggaacg ctcttcagct tttcggtgtc tactgaaatc
      541 aaggtattaa tgggaataac agcctaagat atttggagtt cagagagggc gcgtttgatt
      601 ttcaaacttc gcaacagaac cgaaaattta taccggaaga ttttcaaaac ttacagttag
      661 aaaacatgcg tttagaagat ttatttagat tagcagatga tgataagtat agatttgaag
      721 ttttgaaaat taaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
      781 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
      841 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
      901 taacagatat ctacgtagaa gatgaagtta taatcaaata tatgaagcct gtgataaaac
      961 ttgttaataa aaataaagga aacggataac actttacaat gctgttttgg tactccaatt
     1021 taaacaaaac aatcgaattt acgtacttaa ttaagtacgt tagaatggag atataaatta
     1081 gagggggagt agatatggca aaaacagtct taaatcctag ctcagcacgg aaaaattttt
     1141 ataagttgtt gaaagatgta aatgagaatc atacggaaat tgaaatcatt agtgagcgca
     1201 gtgaaaataa tgcggtgctt attggtttgg atgatttgaa agcaatcaaa gaaacgctat
     1261 tacttgagca aataggcact ttgggtgttg ttcgtaagcg agaaaaagat gatagtggat
     1321 ttactaatgt tgatgaaatt gattgggata acttataatg aataactatt ctgtgatgat
     1381 taaaaactcg gctaaagctg atttaaagaa aatcaaacag tctaatttga aacctcaatt
     1441 tgagaaggtt attcagactt taaaggaaga tccctatctc cctacccaat catttgagaa
     1501 gttaaaacct acatatgaag gtaggtattc tagaagattg aatagacaac accgggttgt
     1561 ttataaggta gatgaagatg aacacgtggt tgaaatttat tcagcaagga cgcattacga
     1621 gtcacagtaa ttacttgtaa atacattatc tcgtggtata atgtatttac taagtaatta
     1681 catactgtta gaaatgaggg atttaaatga ttacaaaaac aagaaaacaa ggcaattcaa
     1741 tcatgttgac agtacctaaa gattttaatg ttcctaatgg agtcgaagtt gaagcaaagt
     1801 tggtagagaa tggtattctt tatgaatttg ttgaaccaca aaaagaattt tatgatttta
     1861 gcgaagatat tttgtctgat ataattgcag aaggctacga taaagatgag attcttgtag
     1921 aatttaagaa tagaaaaaac aaaatgcatt cttcctttag agatattgct gaagatacac
     1981 ttactaattc taaagtaatg actaaagagg agttggcaac agaaattggc ttatgaaatc
     2041 gttccttcaa aacatgtcat taagtattta aaaaagctaa aagagaagcc attgaaagaa
     2101 aaatttttga atttaattta tgatgaaatt gcgattgatc catataaggg tgaacaaaaa
     2161 actggggatt tatcaggtat ttggtctagt gggttcaaat atgcgggcac aacatataga
     2221 gtagcttacg agattataga aaacaaggtt attccagtat tgttatgtgg aacgcatgaa
     2281 aacttttatg aacagttgaa aaaattaatc tagtgatttg ttgtcgttct taagaaggac
     2341 ggcttctttt ttatcgaaaa tctgattcgt ctacatgact aaagtcgcga gtattctctg
     2401 cttctcgata aattaaataa cgtcattact ttgaggaaag ctataaatca atatatttca
     2461 acttttcctt atttatgtac taccttttac tta
//
LOCUS       DACEJZ010000173         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEJZ010000173 DACEJZ010000000
VERSION     DACEJZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.186"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1014..1304)
                     /locus_tag="IW656_002849"
     CDS             complement(1014..1304)
                     /locus_tag="IW656_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838512.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IW656_002850"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IW656_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACEJZ010000174         2482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEJZ010000174 DACEJZ010000000
VERSION     DACEJZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.187"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..254)
                     /locus_tag="IW656_002851"
     CDS             complement(9..254)
                     /locus_tag="IW656_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838513.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(271..1122)
                     /locus_tag="IW656_002852"
     CDS             complement(271..1122)
                     /locus_tag="IW656_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0838514.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1288..2292
                     /locus_tag="IW656_002853"
     CDS             1288..2292
                     /locus_tag="IW656_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0838515.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          795 a          424 c          498 g          765 t
ORIGIN      
        1 cattctttct attttttcgg tatcaaggca aaaacaggta gttcaggtga ataaatgact
       61 tcatgttcgt gccagggttt gaccattagc gaatgatcga accgtttttt cagttcttcg
      121 atgatttgat catgtgtcca gtttcttgca ggaaaatgtt ggattttctc tgcatcgatc
      181 aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt ccgagagaga attcaagcgt
      241 tcgattggat ccataaaatc acctttcgac ttaaaattgg gtagtatctg ggtcttttgc
      301 tttgccgact acaccatcta actgatcgat ttgtttcatt tcttcttctg ttaattcaaa
      361 atcaaataat tcagtatttt ctttgattct gcttggtgtc actgacttag gaagaggaag
      421 gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt catgtgcttc
      481 agcgatttgt ttcatttcag gcacatcgaa gattttacca gtgcctaatg gactatatgc
      541 ttcgaggatc atatcatgtt tttttgcata ctcaactaca gttgtctgta attcgcctgg
      601 tgcaaggaaa acttgattga ccatcggcat aatttttgct gtttttgcta gtgtatctag
      661 atggtggggt aagaaattgc tcacaccgat tgctttgatt ttacctgctt catacagttc
      721 ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa aagcaacagg
      781 gtttggccag tggatcaaaa acagatctaa ataatctgtt tggagcttct tcaatgattc
      841 ttcaaaagaa gacatcacaa gttcatagct gtggtttgca ttccataatt ttgttgtcaa
      901 gaatatctct tctctaggaa ttccactttc cttaatcgct tggccgacgc tttcttcatt
      961 cttgtatcct tgggcagtat cgatatggcg ataacctgca gccagcgctt ctttaacaga
     1021 ggaaacagcg acatccccat caggtgtttg ccatgtgccg aatcctacta ccgggatttc
     1081 gtaaccattt gatagacgat aagtgcttgt taatgaattc atactttcag ctcctttttt
     1141 attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt tgtttctact
     1201 cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat tttttttccg
     1261 taaactaaga cagaagggaa tgagaaaatg gatatctatt taaaaaaagc aattctgcat
     1321 attattgaca gagaaactgg ttctccagtc ttttcacaaa aagagctgga tctgacaaaa
     1381 gaatatattc gtgattttct tcaaaagaag atccaaaaaa tctcttctgc tcaaacaaaa
     1441 actggacaac tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc tcaagaaaat
     1501 tttgtggaag caagtgaagc aatcgttcag cggtggttcg atatctacca agaaagtgaa
     1561 gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat tagatacagt gatgtactta
     1621 tctttattga aagtcaatta tcgtgaggct tacactcact ttgttgaagc agacgaagca
     1681 gggatcgaca accgcttgat tttgaatcga gcaattctag gggcgaaaac acaaaaagca
     1741 gatgaagcat tcgctgtgaa tttgactgat tggacctacg aattaattga aaaaaaatat
     1801 gagttttctg gcaaaaaaga gctttatttt tctggaagag tgatcgaaag tgttcctgca
     1861 ccttctttag aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct tggaaaaaaa
     1921 tttgagacag aagaatttga tatcatggct gatttgaaag aagctgtcta tgatacgata
     1981 gaagaaaaag gtcggttgga tcatgagatg attgcggaaa aagtcttcaa ggaaaacatc
     2041 acggcaaaaa tggcttttaa agaagaagta caagagcaag gatttgtccc agaagcacct
     2101 cctgtaaaag aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa attcaagcta
     2161 tcaaacggga tcgaactgat cgtaccagta gatgtatatc gcaacccaga tttgattgaa
     2221 tttgtgaata atccggatgg tacgatatcc gtgatgatca aaaatgtgga tgaagtattg
     2281 aatcgtttat aaaaatgact agaatattag gtgttcaagt accagtgatc agttaaatga
     2341 attgtgtcaa gagaaggatc ggaaaagacg aatataacaa aaaaagagcg tgagacaaaa
     2401 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact
     2461 ccttcttatt tcttggagtt aa
//
LOCUS       DACEJZ010000175         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEJZ010000175 DACEJZ010000000
VERSION     DACEJZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.188"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW656_002854"
     CDS             206..2068
                     /locus_tag="IW656_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0838516.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IW656_002855"
     CDS             2068..>2399
                     /locus_tag="IW656_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0838517.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEJZ010000176         2353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEJZ010000176 DACEJZ010000000
VERSION     DACEJZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.189"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..363
                     /locus_tag="IW656_002856"
     CDS             91..363
                     /locus_tag="IW656_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838518.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            808..987
                     /locus_tag="IW656_002857"
     CDS             808..987
                     /locus_tag="IW656_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838519.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1046..1402
                     /locus_tag="IW656_002858"
     CDS             1046..1402
                     /locus_tag="IW656_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838520.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          845 a          327 c          447 g          734 t
ORIGIN      
        1 ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt gtcggtagct
       61 gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa
      361 taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc
      421 aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa cgcctagtaa
      481 ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
      541 gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca tttgaacaag
      601 attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga tagtgcagct
      661 gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat cttttggcgg
      721 tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt tttcataggg
      781 ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa acggttaccc
      841 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtatggcaaa
      901 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
      961 tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat ataagtgata
     1021 tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt acaaattaaa
     1081 gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc atttgtatag
     1141 agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag ctggggatcg
     1201 atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc tttggcttat
     1261 tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact tagataagct
     1321 aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt tagaaagtga
     1381 cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat tattttatta
     1441 agaatgatgg aatataagga taaggttacc aacataacga tcattacttc tctgatatgc
     1501 tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc
     1561 ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta
     1621 tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa
     1681 gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga
     1741 catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat
     1801 gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa
     1861 gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa
     1921 atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt
     1981 gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac
     2041 atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg
     2101 attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa
     2161 tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc
     2221 aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga
     2281 tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt
     2341 aaaggttttt aat
//
LOCUS       DACEJZ010000177         2211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEJZ010000177 DACEJZ010000000
VERSION     DACEJZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.190"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW656_002859"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW656_002859"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0838521.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          589 a          479 c          404 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACEJZ010000178         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEJZ010000178 DACEJZ010000000
VERSION     DACEJZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.191"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            278..802
                     /gene="infC"
                     /locus_tag="IW656_002860"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="IW656_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0838522.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="IW656_002861"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="IW656_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0838523.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="IW656_002862"
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="IW656_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0838524.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1838..>2194
                     /locus_tag="IW656_002863"
     CDS             1838..>2194
                     /locus_tag="IW656_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0838525.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          735 a          384 c          479 g          596 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggt
//
LOCUS       DACEJZ010000179         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEJZ010000179 DACEJZ010000000
VERSION     DACEJZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.192"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..528)
                     /locus_tag="IW656_002864"
     CDS             complement(325..528)
                     /locus_tag="IW656_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838526.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IW656_002865"
     CDS             complement(987..1952)
                     /locus_tag="IW656_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0838527.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEJZ010000180         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEJZ010000180 DACEJZ010000000
VERSION     DACEJZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.193"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..424)
                     /locus_tag="IW656_002866"
     CDS             complement(<1..424)
                     /locus_tag="IW656_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0838528.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IW656_002867"
     CDS             complement(533..1564)
                     /locus_tag="IW656_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0838529.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          655 a          337 c          360 g          750 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatta tatgaaattt ttcataaaaa
     2101 tt
//
LOCUS       DACEJZ010000181         2099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEJZ010000181 DACEJZ010000000
VERSION     DACEJZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.194"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            169..1539
                     /locus_tag="IW656_002868"
     CDS             169..1539
                     /locus_tag="IW656_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ0838530.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IW656_002869"
     CDS             1560..1844
                     /locus_tag="IW656_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838531.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          728 a          307 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttc
//
LOCUS       DACEJZ010000182         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEJZ010000182 DACEJZ010000000
VERSION     DACEJZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.195"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..267
                     /locus_tag="IW656_002870"
     CDS             <1..267
                     /locus_tag="IW656_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0838532.1"
                     /translation="KIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVK
                     EMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            268..1020
                     /locus_tag="IW656_002871"
     CDS             268..1020
                     /locus_tag="IW656_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0838533.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1025..1972
                     /locus_tag="IW656_002872"
     CDS             1025..1972
                     /locus_tag="IW656_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0838534.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          724 a          271 c          403 g          660 t
ORIGIN      
        1 aaaattatag aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg
       61 tttactttag atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa
      121 aacattgtta aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat
      181 ttctttgaaa acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa
      241 gtattgaaaa tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct
      301 gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat
      361 ttcgctttag caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact
      421 tgtttgaata cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg
      481 aaaattgtag aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata
      541 cgtgattcat tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta
      601 gatttgactt cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta
      661 aaaaatttag ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt
      721 aatatggtaa aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct
      781 aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa
      841 tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat
      901 tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct
      961 aaatttttag aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa
     1021 aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa
     1081 tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga
     1141 aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt
     1201 gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc
     1261 tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt
     1321 attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag
     1381 ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat
     1441 tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta
     1501 ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa
     1561 tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa
     1621 acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga
     1681 ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc
     1741 actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca
     1801 tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg
     1861 tgatatccat gactctctag cagatattac taagatccag tcaattggct atcaaccgaa
     1921 atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact
     1981 aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa
     2041 aatcaaatct gggataga
//
LOCUS       DACEJZ010000183         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEJZ010000183 DACEJZ010000000
VERSION     DACEJZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.196"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW656_002873"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW656_002873"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0838535.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW656_002874"
     CDS             complement(1431..1823)
                     /locus_tag="IW656_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0838536.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEJZ010000184         1746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEJZ010000184 DACEJZ010000000
VERSION     DACEJZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.197"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..507
                     /locus_tag="IW656_002875"
     CDS             133..507
                     /locus_tag="IW656_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0838537.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IW656_002876"
     CDS             complement(621..1250)
                     /locus_tag="IW656_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0838538.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IW656_002877"
     tRNA            complement(1384..1456)
                     /locus_tag="IW656_002877"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IW656_002878"
     tRNA            1670..1743
                     /locus_tag="IW656_002878"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
BASE COUNT          602 a          256 c          307 g          581 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaa
//
LOCUS       DACEJZ010000185         1637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEJZ010000185 DACEJZ010000000
VERSION     DACEJZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.199"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(184..1503)
                     /locus_tag="IW656_002879"
     CDS             complement(184..1503)
                     /locus_tag="IW656_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ0838539.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          525 t
ORIGIN      
        1 aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa ctgattttga
       61 ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat tttgggtgta
      121 aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg ttagattgtt
      181 ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga attcattctg
      241 atacacatga taactagata atttgagata gactcgtcta cccgtttgga ctaatttccc
      301 agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt ggacttcttc
      361 aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga gcatcattcg
      421 gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc cagctttcgc
      481 ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga tggtatcagg
      541 agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc
      601 ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg cttgataagt
      661 ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat aaagtactga
      721 tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat aatgactctt
      781 ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa
      841 gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga gaaactcctt
      901 tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga agtcaccagt
      961 caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg tattataatt
     1021 cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa tgattaattc
     1081 cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag cttgaagttc
     1141 atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag
     1201 ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat tagctgcaga
     1261 atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg ataagttgtc
     1321 atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt tgcttagcca gttcagaaaa
     1381 accaaagatg ttcatcagct ctttcactag gacgagaccc gaatcactcg ataattgacc
     1441 acctgtatga gaaatagtga tatttgaatt gaattttact tggtttttgt gtaagctagt
     1501 catgaaaaga actcctttct tttggttggt ttagtcactt taaccatagc agaaaggggt
     1561 tcttttttca tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc cgtgaggcag
     1621 tttcacatgg ttttaat
//
LOCUS       DACEJZ010000186         1582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEJZ010000186 DACEJZ010000000
VERSION     DACEJZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.200"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            129..1298
                     /locus_tag="IW656_002880"
     CDS             129..1298
                     /locus_tag="IW656_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0838540.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          569 a          254 c          258 g          501 t
ORIGIN      
        1 aatatcccat attttaaaat aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag
       61 ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca cacagaaatt ttataggagg
      121 actttctcat gactaaattt actatagaaa ttatgcaaac actactcaat aaaggggatt
      181 tagatgaatt atttcaggat catttagagc gagcgattaa ttcacttcta caagcagagc
      241 taactgcttt tttagattac gaaaaatatg atcgaaatgg attcaattca ggaaattccc
      301 gtaacgggaa ttattcacgt acatttaaaa cagaatatgg cgagttgaat ttaactattc
      361 ctagagatcg gaatggcgaa ttttctcaac aaacactacc agcatataag cgaaccaatg
      421 attcattaga aacgaccatt atccaattat ttcaaaaagg cattactatg gctgaaatct
      481 ccaaattaat tgaaaaaatg tatggtcacc actatacgcc acaaacaatc tccaacatga
      541 gtaaattggt ggctgaagat gttttagctt ttaaagaaag aacgttagaa gccaattatt
      601 ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag
      661 cagtgtatat tacgattggt attcgtttgg atggaaccaa agaagttctt gggtttacta
      721 ttgccccaac tgagtctgct tatatttgga aagaagttct tcaagatctt agaaaacgtg
      781 ggttagaaga agttttatta gtagtgacag acggattaag ctgtattgaa gaaagtatcc
      841 atagtgtgta tccgaatgcc caatttcaac aatgttgtgt gcatgtatct agaaatatcg
      901 ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc
      961 aagcgaattc aaaagaagag gcattggatc acatcgactt tatgactagg aaatggaaaa
     1021 agcagtatcc aagagtcgtc aatttactct tgaatcctgc cctattaacc ttttataatt
     1081 tccctcacgc catcagacga acaatttatt cgacgaacct gattgaaggc tttaataagc
     1141 agctaaaacg atatactcga agaaaagaac aattccctaa tgaagaatct ctagagagat
     1201 tcttcatttc tcaatttaat caatataacc aaaaattttt aggtagaatt cacaagggat
     1261 ttaaagaaat tcaggataca ttagagtcga tgatttaact gtaattaaca gaatggattt
     1321 tccatttaca tataattctt gacgctacca taatctaaat aaccatcata ttcatctact
     1381 acttatataa ttgagtttta ttaaactttt taagtttcta ttctatcaaa tatttaaaca
     1441 atctctgcat ccgaaaagac tacctcgtat agttttacta acaatatttt tattagcgtg
     1501 ttcaaatcct gatttcttgt atcaattatt ttctgttcac tttcaataag aaaaaagtct
     1561 ttacttccat ggataataat aa
//
LOCUS       DACEJZ010000187         1560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEJZ010000187 DACEJZ010000000
VERSION     DACEJZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.201"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(215..1198)
                     /locus_tag="IW656_002881"
     CDS             complement(215..1198)
                     /locus_tag="IW656_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0838541.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT          478 a          282 c          235 g          565 t
ORIGIN      
        1 aaattgcttt tcaaattttt gtgaatttcg gcttatttaa gaagaagttg cttctgctcc
       61 caccgtttat tcgtttttaa agaatgagac aaaagtgttt cttacttttg tcccacgctc
      121 ttattcatgc tcagagcgcc aatagataca aaatcatagc caaagtttca cgaaccctag
      181 ctgtcaaaga atcgatattt tattttaata taggctattt taaaataaat ggtttaaggt
      241 attcctctac aaaagccact ctatcaatta tcatgggttt aaaataagaa gtgatggaga
      301 taaccagatt gttcttagcg tcaatataaa gaacatttcc actattacca attgctgaat
      361 atatttcgtt ctcagaatct aatacccacc ataaatagcc atatctcata ttttgtaagt
      421 cttctaaagc actggttcta ggtttgaaca tctcgtttat ccacatgggt ggtacgattt
      481 gttttccttt atacatccct ttatttaggc ataatacacc aatttttgcc agatcacttg
      541 ccgataaaca caaaccatat cctgccgtcc cgatatttag tggatcacaa aaccagacat
      601 tggttttagg agacttatcc attgtaaact gcttatgttc ttcagctgtt tcagcataat
      661 aattgacatg gtttggtatc cctataggat caaatagata cttattggca aaatcaacag
      721 ttgttagctt acttgtctga tacaacaagc cagttaatat gtgtaaacag acagtttgat
      781 agttgaattc gccggttata ccttttcttc ctcccaataa atctagtgat gtttttgtcc
      841 aatcttcact tgaacagact tttgtccatg gatcgccttt acatttgtag ggtgctttca
      901 ttgttagcaa gtgcttgatt gtaatagcgt aaatagtttt ctccccgcgt ttcactttat
      961 actctggaaa ataatctaat actttatcat cgatactttt gattaatcct tgctctaaag
     1021 caatgccaat cagcaaagat acaatacttt tcgttgctga catgatatga caagtatcgt
     1081 cttttttata accattccat tcatctgtat aaacttctac cccatcctta tatgcgacaa
     1141 tttgacatat atttggttgt cgaaaaccaa taaaatcatg caactcttgt ttattcataa
     1201 gatattacct cactttcttc tataagcata atgtcattaa aaatcgattt caattttttc
     1261 gattcttcac gtaatacagg tattcttatg gactagacag cgctttggga agatggattc
     1321 tctgaaaaat ataaaggttc actttatgag tggataaaaa aagcagcaaa tacttcgact
     1381 tctttaattt accaacatta gtgaaaatcg attcatctgg agatttgtaa tttagagtcc
     1441 ttgtcttgat tagaggtgta taaagctttt tattagacat ttttaaattc cattacaatt
     1501 tgcttataaa ctaggttttt aaaggtgact atggtaagat aatagagata ttattttgtg
//
LOCUS       DACEJZ010000188         1517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEJZ010000188 DACEJZ010000000
VERSION     DACEJZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.202"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..566)
                     /locus_tag="IW656_002882"
     CDS             complement(159..566)
                     /locus_tag="IW656_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838542.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(585..1403)
                     /locus_tag="IW656_002883"
     CDS             complement(585..1403)
                     /locus_tag="IW656_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0838543.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          456 a          244 c          205 g          612 t
ORIGIN      
        1 acttatactt taaataataa ttttataatg ttgatttaat cgcaaattta cacgttgttt
       61 attaagttat accttaaata tttaatgtat aacttatact ttaatattca tttatctagt
      121 ataacttata ctttaattga ataccgcatg aaaaaaaatt attttggtaa tttttcaaac
      181 tcttcttgta tttcttctct tatgtgttct tcttttgcta ctcctagtct atgctggata
      241 tatgagtctg ctaatgtatt aacaattttt tcgaaagaaa ttctcccttt ttcactttgt
      301 aactccgtca tatagtcttg taaaattgag agtttgctat ttaatgccgg agagatcttt
      361 ttaggaactg ataccttgtt tcttttatca ggaattgaaa ctggtcggcc tctttttttc
      421 aatgtaatat ttcttgtgtt actcaatgca ctattgctaa agttatattc accgcttatt
      481 ttcacatctt ggcgtttgtt attgggtaat aatggctcaa attcaccatt ctccatcatt
      541 ttaggaatat caaaatcttt aggcattact tatgcaactc cttattattt atttaaaaac
      601 tcttgtctag catctagttc tgccaaaata tttataaata cttggtgtgc tttataatcc
      661 cattgatcaa aatttccatt tttgtatttt cttaaatgta taccaatttt agaatacctt
      721 ctcaatcttt tttgataatg aatgagattt ttggatacca cggttccgta tttttcaatt
      781 gcttcttcta ggatttcttc atcaaaatca tcatttggtt ctaccataca aggtacaata
      841 tctataattt gcaaatcaag atcgtatcta tcgaccatga atttttgata atttacatat
      901 ttgttgactc cctctaagga ttcttcagct gtttgaaatg aaattatcga atagtctgaa
      961 gctgccatag cattatcgga aaattcgcta atggttggtg gtacatcaat aaatatttct
     1021 tgataattgt tctttattgg aagaagtaat tttttcaaga cagtaacttg gtctaattca
     1081 ttttcatgtt tattagcaaa agcggagaat gatcgaaaac tagtgtcaca tgccagaagg
     1141 tcaagatttt catttacctt aataatacta cttgctaatt ctccatttgc gacaccattt
     1201 gttatagtgg ttttgatatc ggttataccg aaagttcttg ctatgacttg tgttaagttc
     1261 gcttgagggt ctaaatcaat aataagcgtt tttctatttc ttatcattgc attgtcataa
     1321 gcaagcatgc tgactatctt agttttacct actcctcctt taaaattgcc aactgtaaat
     1381 gtagtccctt gaaaagtatc catgtaaatc ctcccaatct ttaattgata attaaagtat
     1441 aacttatata tcggaaatgg tcaacattaa aagtataact taaataataa tatctaaaaa
     1501 cgtttggata tagcggt
//
LOCUS       DACEJZ010000189         1480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEJZ010000189 DACEJZ010000000
VERSION     DACEJZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.203"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            364..918
                     /locus_tag="IW656_002884"
     CDS             364..918
                     /locus_tag="IW656_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0838544.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1172..>1480
                     /locus_tag="IW656_002885"
     CDS             1172..>1480
                     /locus_tag="IW656_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838545.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          589 a          157 c          256 g          478 t
ORIGIN      
        1 aaaagcttgg tagcacggtt attcatatgc taatttattt cactttatag taaggggagt
       61 tcttagtata ttgtccaaat ttaaatttca tataatttaa ttatgtaaat tagctattct
      121 aagaacttct acggataact gcccgtctaa aatttaatta agagcttaga atcttcatat
      181 aagtaaggtt aatttttttg tacaaaatag tcggaatatt ctgagttact gaaatgatat
      241 aaaatcataa actaaacccc ttgattttct tcacttttaa cagagtatat atataagttc
      301 atgattatgg taaaatatat atataatcat gaactcagaa tatttttaag aagggaaaca
      361 atagtgagct ataatgtaca accgttaagg acaaatgaag agatacaaga ttttcagttt
      421 tggctaagaa gaacaagtaa tccagaaaga gatagttttt tatttttgtt tggaatcaat
      481 aatgggttac gtatgtcaga tattatagga ttaaaagttg gagacatacg aggtaaaagt
      541 aagcctatta ttgttgaaca taaaacaggt aagcgaaaac cgatttttat agataatttg
      601 agagaagaga ttttattata tactgaaggt aaagaagaaa atgattggtt atttccaagt
      661 cggcaacaag gtcgacatat aacaagggat cgtgtctatc agatatatgc agatatagca
      721 gaaaaattag gaagagatga tataggaaca catacattaa gaaaaacttt tgggtatcac
      781 tactacaaga aaactagaga cattgctacc ttaatgttta tttttaatca tagctcgcaa
      841 gcaattacaa aaaggtacat tggtataact gaagatgaaa taggggcatc gttacgtggt
      901 tttaaattgg gaggctaatt gagatattag gatatatttt ttgtgtataa tgaaaactta
      961 gaaggtgaga ttaatgaaga taactgaatt ttcggaagta tttaaaacgt ttagtaacat
     1021 ttacaataga ataccagaga tagaacaaaa tgttgtagtt gataatgatt attatataga
     1081 gaatgatttt aataaaccaa gaaaatatga caacctatac aattttatac acaatagaac
     1141 aacaataaat aacaacagta acactcgagt tttgattaat tcagcatatt ttcatattaa
     1201 atatttaaag aaatatgagt atgaagaagt tgtattagat tatgggctaa atagacagtc
     1261 atttttaatt tatgcaataa ataacggaaa tttgccaagc aataaaaaga acataattat
     1321 tgaaacatat gcctatctgg aggggggatc taaaaaacta attaagtcat gtaatataga
     1381 gtatcaatta tcagtaggag aaattaaatg tgttttcgaa gaagatttag aacttgactt
     1441 aaaaaaaatt tttgccagca ttcatgatta tataggtttg
//
LOCUS       DACEJZ010000190         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEJZ010000190 DACEJZ010000000
VERSION     DACEJZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.204"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            108..1040
                     /locus_tag="IW656_002886"
     CDS             108..1040
                     /locus_tag="IW656_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ0838546.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IW656_002887"
     CDS             1112..>1456
                     /locus_tag="IW656_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838547.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACEJZ010000191         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEJZ010000191 DACEJZ010000000
VERSION     DACEJZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.205"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW656_002888"
     CDS             complement(150..1337)
                     /locus_tag="IW656_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0838548.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACEJZ010000192         1419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEJZ010000192 DACEJZ010000000
VERSION     DACEJZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.206"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            92..1387
                     /locus_tag="IW656_002889"
     CDS             92..1387
                     /locus_tag="IW656_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0838549.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          279 c          266 g          424 t
ORIGIN      
        1 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
       61 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      121 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      181 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      241 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      301 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      361 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      421 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      481 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      541 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      601 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      661 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
      721 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
      781 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
      841 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
      901 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
      961 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1021 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1081 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1141 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1201 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1261 cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg cgtacggctt
     1321 tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa catttttaac
     1381 caaataaaca gaacagaaag aggttttctt ctttctgtt
//
LOCUS       DACEJZ010000193         1392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEJZ010000193 DACEJZ010000000
VERSION     DACEJZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.207"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(196..1179)
                     /locus_tag="IW656_002890"
     CDS             complement(196..1179)
                     /locus_tag="IW656_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0838550.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          392 a          301 c          265 g          434 t
ORIGIN      
        1 tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctg atataaacgg
       61 tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga agagctattt
      121 ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc taattcacgg
      181 tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt caaaggtctc
      241 taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc gcaagtcatt
      301 tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg tgatggaacg
      361 cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta gtccgatcag
      421 ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct tgaaaatccc
      481 tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc ctgtctggcg
      541 atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt cacgtaaagc
      601 aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt tacgcgtacg
      661 caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc attcttccat
      721 ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac cgccagcttt
      781 agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac tgccttttcc
      841 acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt ggaagcccat
      901 ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc aaatatgggc
      961 aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt cttttccatc
     1021 tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg gtgctgctgc
     1081 atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg ctaaaacaaa
     1141 aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac tctccattct
     1201 atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag gaaacaaaaa
     1261 aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa gactgtgaca
     1321 aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg catttgcggt
     1381 aataagtcca ac
//
LOCUS       DACEJZ010000194         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEJZ010000194 DACEJZ010000000
VERSION     DACEJZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.208"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            192..446
                     /locus_tag="IW656_002891"
     CDS             192..446
                     /locus_tag="IW656_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0838551.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="IW656_002892"
     CDS             447..764
                     /locus_tag="IW656_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0838552.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          492 a          196 c          267 g          418 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaagaca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccttagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacttccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tat
//
LOCUS       DACEJZ010000195         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEJZ010000195 DACEJZ010000000
VERSION     DACEJZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.209"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..1334
                     /locus_tag="IW656_002893"
     CDS             33..1334
                     /locus_tag="IW656_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0838553.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          445 a          275 c          252 g          396 t
ORIGIN      
        1 atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatcat atcaaactac
       61 tacttggaat caaagataaa aacattttca tcacaaatgt ggaatcgaag agcattaaga
      121 atatcaagag tcttgttgtt tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt
      181 gtaaacagat gaatcatgaa ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc
      241 aacttaataa atgtgcgaac caactgactt atcttgcttt agcgaaacaa cgctaccact
      301 gtcgtggctg ccacacctat tttacggcga atacctacat tgtcgataga aactgcttta
      361 tcgctaaaca agtgcgctat aaaattttag aagagctaac tgaaaaacaa gcaatgacga
      421 caattgctaa gcattgcggc gtttcttggt cgaccgtttc tcggacactc gcttctctga
      481 tccccatgac aaaagtaaaa agaaattggt taccgcgttg tttattaatg gatgaatttc
      541 gttcactgaa aaatcaagtc ggtccctact ccttttcttg tatggatggc gatactggaa
      601 aattactgga tattcttcct tcacggaaga aaaaggctct cgtttcttat tttatgcaat
      661 ttgaacgacg cgcacgattg aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg
      721 cctctctgat aaaggaatgt tttccaaagg caaaactcgt tgtggatcgt tttcacattg
      781 tcaaacatct cattcgtaaa tttgaggata ttcgcgtacg aatcatgaaa agctttgatc
      841 gaaacgatcc aacacaagcc aaatattatc gacaattaaa ggcgttgtct cggcttctga
      901 tcaaacgaca ggacaagctt gtttatgaca aatggaccaa atggcgcaat tttggttggg
      961 cgtatctcac cgaaagtgaa gtggtggatc gtctgctttc gatctctgac gaattacgaa
     1021 tcgcctattt ttactatcaa gaaattcttc aagcgtttca cgacaaagag gcggacactt
     1081 tctttaaact tgtaagaacg atgcccaatt ccgttccaaa agaactgcat tctataaaaa
     1141 aagcttttat aaactatgaa tccggcattc gtttagccct cgaattgcct tattcaaatg
     1201 ccaaaataga aaatctgcat acccacatca aagcattgaa acgagtcgct tatggctttc
     1261 gaagctttcg gaaaatgaag accagaattt tcttactaaa taatctgatt acctatgaat
     1321 caaaaaacat ctaaagtaga aaaggaattc taccttagat gttcttat
//
LOCUS       DACEJZ010000196         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEJZ010000196 DACEJZ010000000
VERSION     DACEJZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.210"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            110..1195
                     /locus_tag="IW656_002894"
     CDS             110..1195
                     /locus_tag="IW656_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0838554.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIVKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          565 a          196 c          212 g          383 t
ORIGIN      
        1 acttatacct taaatactga aagtgtttta ttcaccctta atacatggta tagttcactt
       61 gttgaaaatc ctgaatatat acaagtttct aagaagaagg agaaatttaa tgtctacaga
      121 aaatcaatct caaaaaatga taattaaaaa ttggggatat aaagcaacga atgtttatat
      181 tgaaaaatat tataaatttc ctagtttttt aattcagaat tctaaatact ctaaaatgaa
      241 agacagctca aaaatagcct atatgctttt taaaaatgaa tttcgtagag ccttacatga
      301 aaaatggatc gacgatgatg gtctattata tttggaattt acacaagcag acctaatgga
      361 caaattaaat tgctaccaag gcaaagtaaa aaccatttta aacgagttag aaacatataa
      421 tttgatttca gtcgtaaaag gagaatttaa cccaaaaacg aaaaaaaatg aaaaaaataa
      481 atactattta cttcaacctg aaataagtgc cgatgatctt tttattcaaa attctttcga
      541 tgacgattta agcgaaacta ttgttaaatc aacctcttct cggaatgcgg aaatcgcacg
      601 tcgagaggaa actgctaaaa cccttgataa cactggaaat gcggaaatcg cacgtcgaga
      661 aaaaacagct gaaacccttg ataacactgg aaatgcggaa atcgcacaag attattatat
      721 taataaatct ttagacacta aaagacactt aaaagacact gaaacagacc aattacaaaa
      781 tcaagttttg ctagataact tcgttgaaat catgaaagaa gacagcataa acacatttgt
      841 accagaaaag gtattaagct tgataaaaac attttctaac acttacgaag aagcccaaca
      901 aaccgtcacc acaatacaca aagcaaaaca taaagccgag gaacttgaga agacggtagt
      961 tgtgtatgaa gaacttgaat cttacggaat tgatgcggat aaaggattat atatgacgtt
     1021 gctgaaagct tatcaaaaac aaaagactga gaaagtaaat aatttgcaga atttgatttt
     1081 tgtgtatgta aaaaactggt ttattgaaaa agctattgcg gcaaagcttg caaacgaaca
     1141 aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggctag agtagttgat
     1201 aaaaagcgat cactataaaa atgatcaatt ttaaattaga acttttttat gattttaatt
     1261 ttattacaaa tgttacaaat aaatagctat tattccggag tttttagatt aaaagtttat
     1321 gaaaacaatc acaaacgtag gttttaaagt cataat
//
LOCUS       DACEJZ010000197         1351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEJZ010000197 DACEJZ010000000
VERSION     DACEJZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.211"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            172..459
                     /locus_tag="IW656_002895"
                     /pseudo
     CDS             172..459
                     /locus_tag="IW656_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="IW656_002896"
     CDS             473..904
                     /locus_tag="IW656_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838555.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="IW656_002897"
     CDS             906..1043
                     /locus_tag="IW656_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0838556.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1080..>1351
                     /locus_tag="IW656_002898"
     CDS             1080..>1351
                     /locus_tag="IW656_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838557.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          509 a          196 c          276 g          370 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccattatgca ttacagacga agaagcagat aaaatcatta
     1021 ataaagataa gagcgcatct taattgatgc gtttttattt aaggtaaagg agttgtcaca
     1081 tgattaattt aggggaatgg ggaatgatag caggatcaat aaccgctatc gtttctttga
     1141 ttttattagt aattagaccg attgctgcat ctttctcgaa aattactgag actctttcaa
     1201 aagtaagcca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
     1261 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat acagaaaaat
     1321 tggtggaaca cacgcaacaa atcaaaactt t
//
LOCUS       DACEJZ010000198         1341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEJZ010000198 DACEJZ010000000
VERSION     DACEJZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.212"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(82..171)
                     /locus_tag="IW656_002899"
     tRNA            complement(82..171)
                     /locus_tag="IW656_002899"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Ser,seq:tga)
     gene            complement(195..268)
                     /locus_tag="IW656_002900"
     tRNA            complement(195..268)
                     /locus_tag="IW656_002900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Ile,seq:cat)
     gene            complement(282..355)
                     /locus_tag="IW656_002901"
     tRNA            complement(282..355)
                     /locus_tag="IW656_002901"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Met,seq:cat)
     gene            complement(383..455)
                     /locus_tag="IW656_002902"
     tRNA            complement(383..455)
                     /locus_tag="IW656_002902"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Ala,seq:tgc)
     gene            complement(475..548)
                     /locus_tag="IW656_002903"
     tRNA            complement(475..548)
                     /locus_tag="IW656_002903"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Pro,seq:tgg)
     gene            complement(558..631)
                     /locus_tag="IW656_002904"
     tRNA            complement(558..631)
                     /locus_tag="IW656_002904"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Arg,seq:acg)
     gene            complement(642..727)
                     /locus_tag="IW656_002905"
     tRNA            complement(642..727)
                     /locus_tag="IW656_002905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Leu,seq:taa)
     gene            complement(744..815)
                     /locus_tag="IW656_002906"
     tRNA            complement(744..815)
                     /locus_tag="IW656_002906"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Gly,seq:gcc)
     gene            complement(827..899)
                     /locus_tag="IW656_002907"
     tRNA            complement(827..899)
                     /locus_tag="IW656_002907"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Thr,seq:tgt)
     gene            complement(917..998)
                     /locus_tag="IW656_002908"
     tRNA            complement(917..998)
                     /locus_tag="IW656_002908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Leu,seq:tag)
     gene            complement(1006..1078)
                     /locus_tag="IW656_002909"
     tRNA            complement(1006..1078)
                     /locus_tag="IW656_002909"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Lys,seq:ttt)
     gene            complement(1082..1154)
                     /locus_tag="IW656_002910"
     tRNA            complement(1082..1154)
                     /locus_tag="IW656_002910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Val,seq:tac)
     gene            complement(1168..1283)
                     /gene="rrf"
                     /locus_tag="IW656_002911"
     rRNA            complement(1168..1283)
                     /gene="rrf"
                     /locus_tag="IW656_002911"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          345 a          375 c          315 g          306 t
ORIGIN      
        1 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
       61 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      121 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catatgaaac
      181 ttacaacgcc acaatggacc ttgtaggact cgaacctacg accggacggt tatgagccgt
      241 ctgctctaac caactgagct aaaggtccag gctcttaaaa atcgcggcgg aggggatcga
      301 acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca ccgcgaaaaa
      361 gatattcatt atttaaatga aatggagcct agcgggatcg aaccgctgac ctcctgcgtg
      421 caaagcaggc gctctcccag ctgagctaag gccccgtgtt taaatttata attatcggga
      481 agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc aagctgagct
      541 acttcccgtt taattaacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt
      601 caaacactct atccagttga gctatgggcg catatttgaa atgccgagga ccggaatcga
      661 accggtacgg tgatcactca ccgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      721 ccccggcgcg attgctttgg caaagcggaa aacggggttc gaacccgcga cccccacctt
      781 ggcaaggtgg tgctctacca ctgagctatt tccgcgtgtt taaagatgcc ggctaaagga
      841 cttgaaccct cgaccctctg attacaaatc agatgctcta ccaactgagc taagccggca
      901 tgtaaaactt aaattatgcg ggtgaaggga cttgaacccc cacgccgtaa ggcgctagat
      961 cctaaatcta gtgcgtctgc caattccgcc acacccgcaa aaatatgagc cgtacagggc
     1021 tcgaacctgt gaccctctga ttaaaagtca gatgctctac caactgagct aacggctcat
     1081 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
     1141 tgagctaaac ctccatggat aacttaagcg tggcgacgtc ctactctcac aaggggcaac
     1201 ccctcactac aatcggcgct aagaagctta acttctgtgt tcggcatggt tacaggtgta
     1261 tccttctcgc tatcgccacc acactgtggt gttatctttt attgagtaaa ttttgttcac
     1321 tcaaaactgg atttgaagtc a
//
LOCUS       DACEJZ010000199         1330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEJZ010000199 DACEJZ010000000
VERSION     DACEJZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.213"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..394)
                     /locus_tag="IW656_002912"
     CDS             complement(<1..394)
                     /locus_tag="IW656_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0838558.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTAD"
     gene            complement(391..1044)
                     /locus_tag="IW656_002913"
     CDS             complement(391..1044)
                     /locus_tag="IW656_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0838559.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          419 a          230 c          124 g          557 t
ORIGIN      
        1 tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc tttatttctc
       61 tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata ttatctttga
      121 tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca ctactcttgg
      181 aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata ttactagaaa
      241 tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt ttaggaaaga
      301 taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa aatatttgta
      361 tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat atcctacatt
      421 tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta tatatacatc
      481 tattactcga tcataaggag tttcttcgct atctttccat acataatcaa taatttgcat
      541 tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta gttcttttgc
      601 atttacatct atttcttcac catttatttt agctgtatag ctattaaaat taaccgatag
      661 atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag aatctattct
      721 agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta aattagtaaa
      781 tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg gtaaattgct
      841 tttctttcta atttctttca acacttctaa accatttata aaaggtatct gaatgtctaa
      901 gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat ctttagcttg
      961 aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta tcatattatc
     1021 atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac tagatattat
     1081 acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
     1141 ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa aggcttctat
     1201 aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt tcttcgtttc
     1261 aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa
     1321 ggagtcgctc
//
LOCUS       DACEJZ010000200         1328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEJZ010000200 DACEJZ010000000
VERSION     DACEJZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.214"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            44..>1328
                     /locus_tag="IW656_002914"
     CDS             44..>1328
                     /locus_tag="IW656_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0838560.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          452 a          259 c          252 g          365 t
ORIGIN      
        1 ttaaatcacc acaaaataaa caactgagag gacgatacgc tatatgaatg attctatcaa
       61 aaaaatgctg agaataacag agaaagattt gatgattaca gaggtctctt acgagaccct
      121 tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag
      181 aagttgtggt tctactgtgg tagatggaaa cgggaaagca attatagtga aaaatggaaa
      241 aaaggaaacg attgtccgtt ttgaacaata caatcatatg cctttggtta tgcgcctaaa
      301 aaagcaacgc tatacctgta aaaactgtcg cacccattgg acggctcaaa gttattttgt
      361 ccaacccaga cattcaatcg caaatcatgt tagatataaa attgcttctt tactgactga
      421 aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta tctttgacca ccgttattcg
      481 tacattgaaa gagtttaaaa gctatttacc aaagcaatct aagaagatcc tccccagagt
      541 attgatggtt gacgaatttc gttcgcatgc ttccatagaa gataagatga gctttatttg
      601 cgcggatggc gaaacaggaa aattaataga tgttttgcct acgcgtaaat tacctcgatt
      661 aacaagctat ttcttaggtt gtaccaatcc agaagaagta gaattcttgg tgacagacat
      721 gaacgccgcc tacttccagc tcaccaaacg tgttctgcca aatgcgaaag tcgtgattga
      781 tcggtttcat attgtcaaac acatgaatca agcgttcaat gagttgcgta tccgtgaaat
      841 gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa aaactgaaaa agaactggcg
      901 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      961 ccgagcacca aaatacccat ttcttactga agcaatgatg attgatcgat tgcttgagtt
     1021 ttctccgccc ctaaaggagg cgtatccctt ttttcatgaa ttagttgaag cctttcgaga
     1081 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacag
     1141 ctttcgggaa aagcttcaaa accttctgac ctatgaagaa ggcatcacca acgcaatgat
     1201 ctatccttat tccaatggaa aaatagaagc gaagaatacc cacataaaga caatgaaacg
     1261 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
     1321 attaatca
//
LOCUS       DACEJZ010000201         1313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEJZ010000201 DACEJZ010000000
VERSION     DACEJZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.215"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            96..>1313
                     /locus_tag="IW656_002915"
     CDS             96..>1313
                     /locus_tag="IW656_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0838561.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          429 a          270 c          239 g          375 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gca
//
LOCUS       DACEJZ010000202         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEJZ010000202 DACEJZ010000000
VERSION     DACEJZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.216"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..1248
                     /locus_tag="IW656_002916"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IW656_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACEJZ010000203         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEJZ010000203 DACEJZ010000000
VERSION     DACEJZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.217"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1244
                     /locus_tag="IW656_002917"
     CDS             <1..1244
                     /locus_tag="IW656_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0838562.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACEJZ010000204         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEJZ010000204 DACEJZ010000000
VERSION     DACEJZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.218"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1231
                     /locus_tag="IW656_002918"
     rRNA            <1..>1231
                     /locus_tag="IW656_002918"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACEJZ010000205         1224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEJZ010000205 DACEJZ010000000
VERSION     DACEJZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.219"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            423..956
                     /locus_tag="IW656_002919"
     CDS             423..956
                     /locus_tag="IW656_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0838563.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          390 a          226 c          229 g          379 t
ORIGIN      
        1 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga gaagttgtga
       61 aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc tcatattttg
      121 ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagaagttgt
      181 gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc ctcacaactt
      241 tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca aatcacaagg
      301 aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct atgttttttg
      361 ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga ggttttacag
      421 aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat agtgcttgtt
      481 ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat acattgatac
      541 agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat caagaagacg
      601 tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa
      661 atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag
      721 attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa agtagccgtc
      781 gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc
      841 aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa atcctgcttt
      901 atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga
      961 atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag
     1021 caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg
     1081 gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag
     1141 aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat
     1201 gcaagctttt ggacaccgtt tacc
//
LOCUS       DACEJZ010000206         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEJZ010000206 DACEJZ010000000
VERSION     DACEJZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.220"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW656_002920"
     CDS             <1..1076
                     /locus_tag="IW656_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0838564.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACEJZ010000207         1181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEJZ010000207 DACEJZ010000000
VERSION     DACEJZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.221"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1163
                     /locus_tag="IW656_002921"
     CDS             <1..1163
                     /locus_tag="IW656_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0838565.1"
                     /translation="EIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRT
                     GFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLF
                     EKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATY
                     IPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSK
                     EEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          432 a          219 c          198 g          332 t
ORIGIN      
        1 cagaaattgt gcaaactcta gtcactaaag gcgatttaaa tgaattattc cgttcgcact
       61 tagaaaaagc gataaacaca ctcctacgga ctgaattaac ggctttttta gattacgaaa
      121 aatatgatcg cactggtttt aattcaggta attcgagaaa cggttcttac tttcgatcaa
      181 tcaaaaccga atatggtgaa ttaacattgg aaatacctag agatcgtaat ggtgagttta
      241 aacaacaaac tttaccagcc tacaaaagaa caaacgatac attggaaacc actattatcc
      301 atttattcga aaaaggtgtt acgatgtctg aaattgctga tttgatcgaa aaaatgtacg
      361 gtcatcacta tactccacaa accatgtcca acatgactaa agttctgact gaagaagtaa
      421 atgcctttaa atccagagcc ttaaatgata agtatgtcgc tatttttatg gacgctactt
      481 acattccact aaaacgtcaa accgtatcca aagaagcgat ttatattgcc attggtatac
      541 gagaagacgg cactaaagaa gtactgagtt atgcgattgc tccaactgaa tcaacatacg
      601 tttggaatga gctgctacag gatattaact ccagaggagt tcaagaagtc ttgcttttta
      661 ttacggacgg cttaaaaggc atgaaagata ctatccatca aatttatcct aaagcaaaat
      721 atcagcattg ttgtatccat gtatctcgta acatcgctca taaagtacgt gtcaaagacc
      781 gaaaagaaat ctgtgatgac tttaaggctg tttatcaagc taactcaaaa gaagaagcga
      841 ataccttctt atccggcatg attgagaaat ggaagaaaaa ctatcctaaa gtgacgcagt
      901 cactcataga aaaccaagac ttattaactt tttatgattt tccacctagc attcgtagaa
      961 ccatttactc aaccaatcta atcgagtctt tcaataagca aattaaaaga tacagccgta
     1021 gaaaagagca gtttcaaaat gaagaatcac tagaacgctt tctagtcagc atttttgata
     1081 catacaatca aaaatttcta aacagaagcc ataaaggttt tcaacaggta accgatacat
     1141 tagtttcaat gtttactgag taactaatta ttttgcagga g
//
LOCUS       DACEJZ010000208         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEJZ010000208 DACEJZ010000000
VERSION     DACEJZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.222"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            75..692
                     /locus_tag="IW656_002922"
     CDS             75..692
                     /locus_tag="IW656_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838566.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            696..1019
                     /locus_tag="IW656_002923"
                     /pseudo
     CDS             696..1019
                     /locus_tag="IW656_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          413 a          167 c          253 g          304 t
ORIGIN      
        1 ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac tcttgagaaa
       61 gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa tctggtgtta
      121 aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa aaaatgatat
      181 caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt ttaacgaaag
      241 ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag acaagcgatg
      301 gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga acatatattt
      361 tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc ttagaagtta
      421 ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct gaagaattaa
      481 agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca attgcagaag
      541 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag tcatatgtag
      601 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
      661 attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta aaaataaaac
      721 aaagcggtgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
      781 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
      841 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
      901 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
      961 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1021 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1081 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtgggga
//
LOCUS       DACEJZ010000209         1077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEJZ010000209 DACEJZ010000000
VERSION     DACEJZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.223"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..996)
                     /locus_tag="IW656_002924"
     CDS             complement(<1..996)
                     /locus_tag="IW656_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0838567.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRW"
BASE COUNT          296 a          228 c          187 g          366 t
ORIGIN      
        1 ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt
       61 tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg
      121 atagccaaca tttccagaag tattataagc ttgatttaca tctgatctat aaattccatc
      181 agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta
      241 tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa
      301 ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg
      361 tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg
      421 agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg
      481 atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg
      541 gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa
      601 ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc
      661 aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg
      721 ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt
      781 ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc
      841 atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt
      901 aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa
      961 caggctcatc gcagtaatag taattttctt tttcatatct atcctcctat ttatctgatg
     1021 aaattttctt atacgttcga tttgatacac ctaaaatact ccctagaaat gctccaa
//
LOCUS       DACEJZ010000210         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEJZ010000210 DACEJZ010000000
VERSION     DACEJZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.224"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(96..437)
                     /locus_tag="IW656_002925"
     CDS             complement(96..437)
                     /locus_tag="IW656_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0838568.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          317 a          245 c          129 g          373 t
ORIGIN      
        1 aaattcctta ctcaatgcct aaggtaatac taccttttta ttaatcatcg tgactccttc
       61 taagccttta tctgtaaaat ttattcctcc agtttttatt cttctttatc cgctacaaat
      121 tcaatgattt caaagctgac aatataatca ctcaaatatg accaagtatc ttcaacagat
      181 aattcgtctc cattatcatt cataagcgtc attttaaacc attcattacc ttcatcatct
      241 tcaggtagca agccgaatcc aattggatta tcagtcaatg tttgatcgta taaatcatcc
      301 tcgtctaatg ctaaaacatc taatccttta caatttccgc ctcttctaaa cgtcacaaat
      361 cctttgtaat cccaacgttg gaaagaaacc ttaatagtat gtgtgcccaa tcgtatattc
      421 ggatcataat ttttcatttg tcattcctcc attacgtaaa tttcattttc tctatgtttt
      481 atatcaattt tttcacttta ttcaggactt gttcatattt tatcgttttt cgtttactta
      541 aaaatacata aaaaatttcg actcgtttat ggcgtccgaa acgagaaacc ctagtagaca
      601 gcggtaaatg ctcgattgat ccaataatca acgtcctcgc aagtatctac atgtactgca
      661 gcacacgaca caatttcttg tgcgtcctcc ccattacgag aagacctact ccttattcca
      721 tgagtttgcg tcaaagtcta gatcccttag tagctgctca tgattaatct agacgctatt
      781 ctaacttgtc taacttctaa attccctctt gtttactctg cgcttattat ttacacaaat
      841 tcatttcacc actatccaaa caagcgcatt tactcgattg accaattttt tacgttacca
      901 atcgttaaaa cctaccccgt cactccccga agggaattgt tgatagatca agtctactcc
      961 ttatttttct agtgactcta agttccttta gaagtctcga caatattttc aagcaagcga
     1021 aaaacgataa aatacaagtt atgagaatga tcaattcagc cgat
//
LOCUS       DACEJZ010000211         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEJZ010000211 DACEJZ010000000
VERSION     DACEJZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.225"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(36..992)
                     /locus_tag="IW656_002926"
     CDS             complement(36..992)
                     /locus_tag="IW656_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838569.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACEJZ010000212         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEJZ010000212 DACEJZ010000000
VERSION     DACEJZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.226"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1035)
                     /locus_tag="IW656_002927"
     rRNA            complement(<1..>1035)
                     /locus_tag="IW656_002927"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEJZ010000213         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEJZ010000213 DACEJZ010000000
VERSION     DACEJZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.227"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            369..1000
                     /locus_tag="IW656_002928"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IW656_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DACEJZ010000214          963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEJZ010000214 DACEJZ010000000
VERSION     DACEJZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.228"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(13..86)
                     /locus_tag="IW656_002929"
     tRNA            complement(13..86)
                     /locus_tag="IW656_002929"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
     gene            complement(641..955)
                     /locus_tag="IW656_002930"
     CDS             complement(641..955)
                     /locus_tag="IW656_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838570.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          354 a          150 c          152 g          307 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      481 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      541 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      601 gtaagaagta ataattcctg tactaaaagg cataattgct ttaattattt tttgacttct
      661 cttcaaacac aagaagtagt tgtgtaataa ttgcagaaat aaggattcca ataatagata
      721 taaaatttga catggaaacg tctataacat taaaagaata aagtgttgaa gaaagtaaac
      781 tgattgaaat aacccctccg taaaaaatag tctgtgaata tacccacttt ttttcagaag
      841 acgtacttcg tttggttcta aaacctatcc ctgcatttct actcttatca aaaaaccaaa
      901 tatttagaag taataatatt gctattatta agataaaatt actgtaaaaa tccaaaagct
      961 atc
//
LOCUS       DACEJZ010000215          962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEJZ010000215 DACEJZ010000000
VERSION     DACEJZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.229"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            312..608
                     /locus_tag="IW656_002931"
     CDS             312..608
                     /locus_tag="IW656_002931"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0838571.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..962)
                     /locus_tag="IW656_002932"
                     /pseudo
     CDS             complement(531..962)
                     /locus_tag="IW656_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          300 a          194 c          157 g          311 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tccccacgcg aacataagta agatccgtaa cgatggcttc caatggctgt
      781 tcttgtgtaa aggtccgatc taatacattc gctgttttcg cttcattaca agctgttcgt
      841 tgccctttaa aatgagcgat cgtataggta gatgtcaatc cgcggcgttt catgattcga
      901 ccgattcggc gccgactgag ttgaagccca cgctttgcta aacacttctt caattttcgg
      961 gt
//
LOCUS       DACEJZ010000216          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEJZ010000216 DACEJZ010000000
VERSION     DACEJZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.230"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>943
                     /locus_tag="IW656_002933"
     rRNA            2..>943
                     /locus_tag="IW656_002933"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACEJZ010000217          923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEJZ010000217 DACEJZ010000000
VERSION     DACEJZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.231"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            118..879
                     /locus_tag="IW656_002934"
     CDS             118..879
                     /locus_tag="IW656_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0838572.1"
                     /translation="MSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFKNKEILKLEK
                     LKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQI
                     LSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKI
                     ILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDSNKENSN
                     GLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          411 a           65 c          123 g          324 t
ORIGIN      
        1 ttattaatat caattttatt ttctgctatt atttctttaa tttatgcaaa atcaataaaa
       61 aataatatac aagaaataaa aattgttact gataaaatga tgaaacttga taaaaaaatg
      121 agtttaaaag ttagctctaa tgatgaagtt ggagaattaa aacaacaaat taatgatttg
      181 tattctactt tattaagaac aattgacgat ttggaattta aaaataaaga aattttaaaa
      241 ttagaaaagt taaaatatga cttttttaaa ggtgcatccc atgaacttaa aacccctctt
      301 gctagtctta aaataatact ggaaaacatg aaatacaata ttggaaaata taaagaaaga
      361 gatatttata ttaatgaatg tattgaaatc gttgatagtt taacaaaaaa tatttctcaa
      421 atattatcag ttcattctat agaaaatttg aataatgatg aagaatattt gaaaataaat
      481 gatacattag aagacatatt aaaaaagtat gaaatattag caaatcaaaa gaaaataact
      541 gtcaataatt atatattaga cgagaatgtt tatataggaa aaacagcttt aaagattatt
      601 ctatctaatt tgattagtaa tgcggtaaaa tatactgatg taaatggggt aattaatatt
      661 gggatagtta atgattggtt atatatagag aattcatacg gtaacaataa aatttcgaat
      721 atggataaaa tatttgatgt taaatttgat tcaaataagg aaaatagtaa tggattaggt
      781 ttatatattg taagtaatat tctgaataat tacaatatag aatataaagt attgcaggat
      841 gaagaatttt ttatctttaa aataaaaata ttttcttaat taagatataa ttttgcaata
      901 gttattgaaa aatagagact tat
//
LOCUS       DACEJZ010000218          891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEJZ010000218 DACEJZ010000000
VERSION     DACEJZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.232"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..235)
                     /locus_tag="IW656_002935"
     CDS             complement(5..235)
                     /locus_tag="IW656_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838573.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(485..685)
                     /locus_tag="IW656_002936"
     CDS             complement(485..685)
                     /locus_tag="IW656_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0838574.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          313 a          142 c          133 g          303 t
ORIGIN      
        1 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
       61 ttttgccttc agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa
      121 aagctaatat agctaaaatt gaaacatcca tctggaattt gtctttttcg ttgctttgct
      181 cataaaaatc tgcatattca tcactaatat tttttctaat aaattcttcc atcataaaga
      241 tcacctcgaa aagagtatac gaacaaacgt tctttttgta aaggtggaat ttattatact
      301 aaataaatag gtgaacaagt acttgtgcca aattatgtgc caaaaaaaat cgaatttaat
      361 aaaacacaaa caaaaagaaa tcctattatg ataacatttc ttataataac aaacacaaca
      421 gaagacgtgt aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
      481 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
      541 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
      601 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
      661 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
      721 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
      781 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
      841 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa a
//
LOCUS       DACEJZ010000219          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEJZ010000219 DACEJZ010000000
VERSION     DACEJZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.233"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..888
                     /locus_tag="IW656_002937"
     CDS             <1..888
                     /locus_tag="IW656_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ0838575.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACEJZ010000220          886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEJZ010000220 DACEJZ010000000
VERSION     DACEJZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.234"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          311 a          145 c          140 g          290 t
ORIGIN      
        1 aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc
       61 ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt
      121 actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag
      181 ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg
      241 attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat
      301 tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca
      361 actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac
      421 tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt
      481 tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg
      541 tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa
      601 taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct ggtgttatag
      661 attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa accgatactc
      721 tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa acttcactaa
      781 cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt atcaaaaatg
      841 ctgctcattt caacttatat gttttagtga actaccccca cttagc
//
LOCUS       DACEJZ010000221          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEJZ010000221 DACEJZ010000000
VERSION     DACEJZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.235"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..>823
                     /locus_tag="IW656_002938"
     CDS             22..>823
                     /locus_tag="IW656_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838576.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DACEJZ010000222          807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEJZ010000222 DACEJZ010000000
VERSION     DACEJZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.236"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IW656_002939"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IW656_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0838577.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          297 a          126 c          152 g          232 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcagg
//
LOCUS       DACEJZ010000223          798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEJZ010000223 DACEJZ010000000
VERSION     DACEJZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.237"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>798
                     /locus_tag="IW656_002940"
     CDS             <1..>798
                     /locus_tag="IW656_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ0838578.1"
                     /translation="IKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTCRNC
                     GSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFV
                     QPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILP
                     RVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFL
                     VTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNEL"
BASE COUNT          255 a          151 c          157 g          235 t
ORIGIN      
        1 ctatcaaaaa aatgctgcga ttaatagaga aagatttgat gattacagag gtctcttacg
       61 agacctttca gaagaaaaag acgttgatcg tcgatgctgt tttctcacct gctccccata
      121 cctgtagaaa ttgtggttct actgtggtag atggaaacgg gaaagtaatt gtagtgaaaa
      181 atgggaagaa ggaaacgatt gtccgctttg aacaatacaa tcacatgcct ttggttatgc
      241 gcttaaaaaa gcagcgctat acctgtaaaa actgtcgaac ccattggacg gctcaaagtt
      301 attttgttca acccagacat tcaatcgcga atcatgtcag atataaaatt gcttctttac
      361 tgactgaaaa agtatcttta tcttttattg cgaaaaattg tcaggtatct ttgaccaccg
      421 ttattcgtac gttgaaagag tttaaaagct acttaccaaa gcaatctaag aggattctcc
      481 ccagagtatt gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct
      541 ttatttgcgc agatggcgaa acaggacaat taattgatgt cttgcctacg cgtaaattgc
      601 ctcgattaac aagctatttc ttaggctgcg ctaatcctga agaagtaaaa tttttagtca
      661 cagacatgaa cgccgcctac ttccagctta ccaaacgtat tctgccaaat gcgaaagtag
      721 tgattgatcg gtttcatatt atcaaacaca tgaatcaagc ctttaatgag ttgcgtatcc
      781 gtgaaatgaa tgaacttc
//
LOCUS       DACEJZ010000224          793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEJZ010000224 DACEJZ010000000
VERSION     DACEJZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.238"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..782
                     /locus_tag="IW656_002941"
     CDS             <1..782
                     /locus_tag="IW656_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0838579.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DACEJZ010000225          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEJZ010000225 DACEJZ010000000
VERSION     DACEJZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.239"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..>791
                     /locus_tag="IW656_002942"
     CDS             94..>791
                     /locus_tag="IW656_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0838580.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDV"
BASE COUNT          271 a          137 c          165 g          218 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa a
//
LOCUS       DACEJZ010000226          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEJZ010000226 DACEJZ010000000
VERSION     DACEJZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.240"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(250..774)
                     /locus_tag="IW656_002943"
     CDS             complement(250..774)
                     /locus_tag="IW656_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838581.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDTHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          261 a          106 c           88 g          321 t
ORIGIN      
        1 ttgacgctcc cttaacaata aatgaagact tttttattgt taattgttta tttttctatt
       61 attagatatg aaaatcgcat ttagataaaa tagaaaaata tgaatatatt ttaaatatta
      121 agatgagagt cattaactta tgatagtagt caatttgctt ttcactaatt acaaaatatg
      181 tgatatagga agtttagtta ttgttctcat aatgccatcc tgtttttaga ttatctagta
      241 atttttttgt tatttaaaat taggagcagt gccaattgtt ttaattttaa aggctttttt
      301 tatatcttct aaattagaag atatttgtat atcaatatgg aatatattac cttttttgta
      361 tttttttatg ttatctttag ctaaaacatc aaatacatgt ccttgaaatt gtatcgaggc
      421 agctggattt agtttaaatt gaggaaactt taccacagct gattcaaaac acttgtcaca
      481 tttttttatt tttttacctt tgtaaaaata ttcgattgaa tattctattt ggcacgactc
      541 acttggcagt atatgtgtgt caatattttt catctgtttc ataggtattt caaaatctaa
      601 actattaaaa acatataatt ccctgtaatt atttacatct aactctacta aatttatctt
      661 tggaacaata gatcctccac ccattccacc ataataaaaa aaatcttgtt ctctatcaaa
      721 atagcacata ttaagctcag tttgtttatc atcctcaata attttataaa tcaaac
//
LOCUS       DACEJZ010000227          766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEJZ010000227 DACEJZ010000000
VERSION     DACEJZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.241"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IW656_002944"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IW656_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0838582.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          222 a          154 c          135 g          255 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgtt tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACEJZ010000228          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEJZ010000228 DACEJZ010000000
VERSION     DACEJZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.242"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(281..478)
                     /locus_tag="IW656_002945"
     CDS             complement(281..478)
                     /locus_tag="IW656_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0838583.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          247 a          125 c           95 g          237 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt acttggtaaa
      541 aggagttttt atagtgtgaa tatatcgctc agattacgtt tcaaattaga ttacttattt
      601 actataacac ggatgtaatg ctaacacaag gataatcact cgatatacgg tttaaatatt
      661 attatttgtc aaattaagcg agacaaaact ttccatttac gtaa
//
LOCUS       DACEJZ010000229          675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEJZ010000229 DACEJZ010000000
VERSION     DACEJZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.243"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            562..>675
                     /locus_tag="IW656_002946"
     CDS             562..>675
                     /locus_tag="IW656_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0838584.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          242 a           99 c          111 g          223 t
ORIGIN      
        1 agtcaaccga tggagaatta gaaacaatct acagtaggga agaaccattt acacaaaatt
       61 attgacgctc ccaagctaaa actcctatta atcgatctac taaaaataaa taaaaaaatt
      121 cttttcaatt ttatttatgg tatgacataa ataaagtaaa gttattcttc taggttaatc
      181 ttattaatac tctttctcac tagtgttgca tgcaacacta gtgagaaaga gtattaaata
      241 tactgcaagg caagacattt ggtaattgaa gaataacaag caaaaaaata gcagtcaggt
      301 ctcttgacaa agtatagttg ctattctttt tttaggccct actttaatga aatttttgtt
      361 tggagggaac gacttcattc ctttgattat ttcagttgaa tttaatagag aagcctggcg
      421 tctattaaga aatacttttc ttgatgagcg tcaatttttt taacggaaat ctatcctata
      481 ctaaattcgg ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa
      541 aaagacagag aggacagagt aatgaatcat tttaaaggca aacaattcaa aaaagacgtc
      601 attattgtcg ctgttggtta ctacctgcgt tacaatctaa gctatcgtga agttcaggaa
      661 ttgttatatg atcgt
//
LOCUS       DACEJZ010000230          646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEJZ010000230 DACEJZ010000000
VERSION     DACEJZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.244"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          221 a          120 c           99 g          206 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata tagataaagt ccgtataatt gtgtaaaagt
      481 aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa aacataccca
      541 ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga ttcaaagcat
      601 tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaaca
//
LOCUS       DACEJZ010000231          617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEJZ010000231 DACEJZ010000000
VERSION     DACEJZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.245"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            250..>617
                     /locus_tag="IW656_002947"
     CDS             250..>617
                     /locus_tag="IW656_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0838585.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQT"
BASE COUNT          250 a           74 c           84 g          209 t
ORIGIN      
        1 aggggcggta cataaaaatt tattatttag attctataaa catgtgcaac ttatctaaca
       61 acaaagtata tattctacag aagtaaacaa atagcttata ttttaggatg tacacaaatt
      121 gacaagtata ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt
      181 tagaacttac ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga
      241 gagaaaatga tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta
      301 aaaaaagaaa ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc
      361 ttatcagcta gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg
      421 attgtttcaa ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat
      481 tatcaagata aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg
      541 ttatatataa cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc
      601 gttacaaccc ttcaaac
//
LOCUS       DACEJZ010000232          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEJZ010000232 DACEJZ010000000
VERSION     DACEJZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.246"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            416..>609
                     /locus_tag="IW656_002948"
     CDS             416..>609
                     /locus_tag="IW656_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838586.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
BASE COUNT          190 a           98 c          139 g          182 t
ORIGIN      
        1 tgaaacacgt tagaggagcg aaaatggagc ctgtaaggcg actggtagcg gaacggtcgt
       61 gttagattgt gtagccgata gctgaacgaa ataaatgctg gcagcgccat tctatttcgg
      121 caggaggagg ctcaaggggt tcggggaatg aaattccctg aggtcttttt attttttcta
      181 gaggagtgct ataatcaaag aaaatgagaa aaaagagtaa aaaaatagtc gagcagtgtt
      241 gacgcactct cgacctttac ggaaactact tcacctagtt ccgtaactca agttcattat
      301 acgaatattt ttaggtttct tcaagctaga gatttttgga tgtggatttt tagagttacg
      361 tgaactctgg aaattctttc caagaatctc ttttttcgtt gaggaggagc tatttttgag
      421 taaaaaaggc ataatcgtaa atgagtggga aaaacttaac cgtatttcta acaggcggtt
      481 aaatgcacaa actatgtgca cccccttaaa aatgcaatgg tcaattgacc gaattaccat
      541 tgttggtaca ttaagggaaa atatttatta tcacactccc catgatgtgt tgattttaga
      601 ttttgaaca
//
LOCUS       DACEJZ010000233          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEJZ010000233 DACEJZ010000000
VERSION     DACEJZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.247"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..580
                     /locus_tag="IW656_002949"
     CDS             <1..580
                     /locus_tag="IW656_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0838587.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DACEJZ010000234          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEJZ010000234 DACEJZ010000000
VERSION     DACEJZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.248"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(318..581)
                     /locus_tag="IW656_002950"
     CDS             complement(318..581)
                     /locus_tag="IW656_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ0838588.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACEJZ010000235          562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEJZ010000235 DACEJZ010000000
VERSION     DACEJZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.249"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>562
                     /locus_tag="IW656_002951"
     CDS             <1..>562
                     /locus_tag="IW656_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0838589.1"
                     /translation="GRAFTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHR
                     YWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKE
                     LPEHYQPVFTTFKKYQTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRER
                     IFLYRGKYFKKTKNTTQLTKARTTTRL"
BASE COUNT          197 a          122 c          110 g          133 t
ORIGIN      
        1 ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt
       61 cggtcgaagc acttaaagaa atactggaaa ctacttcaaa agaatgcgtg ggaattgaaa
      121 ggtcagcatc gctactggcg tccgtcattt cgagatcatc tgacagaagc agagatagtc
      181 gatcggctat tatcttatga tgattcctta aagcgaggct acgaagtcta ccaggatttt
      241 ctttccgtta tcagaagaca agatgttcct gaattcgtcg cgctcctaaa agaagactac
      301 aaggagttgc cagagcacta ccaaccagtg ttcacgacct tcaagaaata ccaaacagaa
      361 atcaaacgag cgttgcgcgt tccctattcc aacggcccta tagaatgttt gaataaccat
      421 atcaaagtac taaaacgaat tgcctatggc tatagaaact ttcaaaacta ccgagaacga
      481 atctttttat atcgaggaaa atattttaag aaaactaaga atactaccca actgactaaa
      541 gccagaacaa caacacggct ag
//
LOCUS       DACEJZ010000236          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEJZ010000236 DACEJZ010000000
VERSION     DACEJZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.251"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..132)
                     /locus_tag="IW656_002952"
     CDS             complement(<1..132)
                     /locus_tag="IW656_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0838590.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACEJZ010000237          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEJZ010000237 DACEJZ010000000
VERSION     DACEJZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.252"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>505
                     /locus_tag="IW656_002953"
     CDS             <1..>505
                     /locus_tag="IW656_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ0838591.1"
                     /translation="RQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLD
                     HSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEG
                     TLRYSLGY"
BASE COUNT          180 a           89 c           90 g          146 t
ORIGIN      
        1 tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa caaaaaaatc
       61 ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc cgattactta
      121 ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat cggaagaagc
      181 gaatcttccc aatatcctag gggacaaagg atatattagc cataaaatcc atgaaaagct
      241 tgcccttaag ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa
      301 actagaccac agcttacttg gaaaacaaag aaaaacagtt gagactgttt tttcttcctt
      361 agaaaaatta ggttgtcaaa atttcaactc acgttctgtt aaagggctag agagtagatt
      421 tgaaagcata ttactggctt acagtgttct attaagtcga gcacaacgac gttttgaagg
      481 aactttgaga tattctttag gatat
//
LOCUS       DACEJZ010000238          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEJZ010000238 DACEJZ010000000
VERSION     DACEJZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.253"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            300..>498
                     /locus_tag="IW656_002954"
     CDS             300..>498
                     /locus_tag="IW656_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0838592.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIE"
BASE COUNT          175 a           73 c          120 g          130 t
ORIGIN      
        1 gagaatttag ctgtagcgag attggtggat atggaggtta taaacaatga agtttaaaat
       61 ctttgaagag gacactcgtt ataaattaga aaaagaatta aacgattttg cgaaaaacaa
      121 tgagattcag catatatctt tagcaacttc taagagaggt tatgcaaatt actatgcagc
      181 tgttgtgagc tatgtaagtc gagaagtgta actcggcaaa taaaaaaagc cggatcgctc
      241 cgactgattc aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga
      301 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
      361 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
      421 ttattacgga tatgccgaag gcaccgaagc acggcaataa ggcagaagac gcgatcattc
      481 aaatgatgga tatagaag
//
LOCUS       DACEJZ010000239          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEJZ010000239 DACEJZ010000000
VERSION     DACEJZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.254"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..414)
                     /locus_tag="IW656_002955"
     CDS             complement(<1..414)
                     /locus_tag="IW656_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838593.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DACEJZ010000240          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEJZ010000240 DACEJZ010000000
VERSION     DACEJZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.denovo.255"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..488
                     /locus_tag="IW656_002956"
                     /pseudo
     CDS             1..488
                     /locus_tag="IW656_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          199 a           88 c           91 g          110 t
ORIGIN      
        1 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
       61 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt
      121 gacaaatggg taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg
      181 cctgaacaaa aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat
      241 gatattttaa agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata
      301 agcatcttta ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt
      361 attatcaatc aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat
      421 ttatccgcag ccgaaaggcc tacggctcaa gaaaaataaa aaaagcctta tcaaaacgag
      481 gcattcag
//
LOCUS       DACEJZ010000241        20985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.051, whole genome shotgun sequence.
ACCESSION   DACEJZ010000241 DACEJZ010000000
VERSION     DACEJZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.051"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..577)
                     /locus_tag="IW656_002957"
     CDS             complement(134..577)
                     /locus_tag="IW656_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0838594.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IW656_002958"
     CDS             complement(795..2135)
                     /locus_tag="IW656_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0838595.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IW656_002959"
     CDS             2404..3300
                     /locus_tag="IW656_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0838596.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IW656_002960"
     CDS             complement(3450..4391)
                     /locus_tag="IW656_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0838597.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IW656_002961"
     CDS             4517..5638
                     /locus_tag="IW656_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0838598.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IW656_002962"
     CDS             5896..7398
                     /locus_tag="IW656_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0838599.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IW656_002963"
     CDS             7465..7992
                     /locus_tag="IW656_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0838600.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IW656_002964"
     CDS             8203..9264
                     /locus_tag="IW656_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0838601.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IW656_002965"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IW656_002965"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0838602.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IW656_002966"
     CDS             10177..12159
                     /locus_tag="IW656_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0838603.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW656_002967"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW656_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0838604.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IW656_002968"
     CDS             12881..14305
                     /locus_tag="IW656_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0838605.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IW656_002969"
     CDS             complement(14486..15259)
                     /locus_tag="IW656_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0838606.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IW656_002970"
     CDS             complement(15256..16161)
                     /locus_tag="IW656_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0838607.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IW656_002971"
     CDS             complement(16315..16635)
                     /locus_tag="IW656_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0838608.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IW656_002972"
     CDS             17109..17795
                     /locus_tag="IW656_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0838609.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IW656_002973"
     CDS             17797..18741
                     /locus_tag="IW656_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0838610.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IW656_002974"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IW656_002974"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0838611.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IW656_002975"
     CDS             19663..20940
                     /locus_tag="IW656_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838612.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACEJZ010000242        20164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.054, whole genome shotgun sequence.
ACCESSION   DACEJZ010000242 DACEJZ010000000
VERSION     DACEJZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.054"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..1049
                     /locus_tag="IW656_002976"
     CDS             9..1049
                     /locus_tag="IW656_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0838613.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1147..1335
                     /locus_tag="IW656_002977"
     CDS             1147..1335
                     /locus_tag="IW656_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0838614.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1393..1746)
                     /locus_tag="IW656_002978"
     CDS             complement(1393..1746)
                     /locus_tag="IW656_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838615.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2277..2699
                     /locus_tag="IW656_002979"
     CDS             2277..2699
                     /locus_tag="IW656_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0838616.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2696..2926
                     /locus_tag="IW656_002980"
     CDS             2696..2926
                     /locus_tag="IW656_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0838617.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3424..3624
                     /locus_tag="IW656_002981"
     CDS             3424..3624
                     /locus_tag="IW656_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0838618.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3651..4844
                     /locus_tag="IW656_002982"
     CDS             3651..4844
                     /locus_tag="IW656_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0838619.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4912..6477)
                     /gene="guaA"
                     /locus_tag="IW656_002983"
     CDS             complement(4912..6477)
                     /gene="guaA"
                     /locus_tag="IW656_002983"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0838620.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6536..7459)
                     /locus_tag="IW656_002984"
     CDS             complement(6536..7459)
                     /locus_tag="IW656_002984"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0838621.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7655..8260
                     /locus_tag="IW656_002985"
     CDS             7655..8260
                     /locus_tag="IW656_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0838622.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8568..9584
                     /locus_tag="IW656_002986"
     CDS             8568..9584
                     /locus_tag="IW656_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0838623.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9725..11026
                     /locus_tag="IW656_002987"
     CDS             9725..11026
                     /locus_tag="IW656_002987"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0838624.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11072..11734
                     /gene="deoC"
                     /locus_tag="IW656_002988"
     CDS             11072..11734
                     /gene="deoC"
                     /locus_tag="IW656_002988"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0838625.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11755..12153
                     /locus_tag="IW656_002989"
     CDS             11755..12153
                     /locus_tag="IW656_002989"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0838626.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12219..13301
                     /locus_tag="IW656_002990"
     CDS             12219..13301
                     /locus_tag="IW656_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0838627.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13513..15075
                     /locus_tag="IW656_002991"
     CDS             13513..15075
                     /locus_tag="IW656_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0838628.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15068..16201
                     /locus_tag="IW656_002992"
     CDS             15068..16201
                     /locus_tag="IW656_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0838629.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16201..17157
                     /locus_tag="IW656_002993"
     CDS             16201..17157
                     /locus_tag="IW656_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0838630.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17256..18422
                     /gene="deoB"
                     /locus_tag="IW656_002994"
     CDS             17256..18422
                     /gene="deoB"
                     /locus_tag="IW656_002994"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0838631.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18446..19264
                     /locus_tag="IW656_002995"
     CDS             18446..19264
                     /locus_tag="IW656_002995"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0838632.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19281..19988
                     /gene="deoD"
                     /locus_tag="IW656_002996"
     CDS             19281..19988
                     /gene="deoD"
                     /locus_tag="IW656_002996"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0838633.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6453 a         3622 c         4333 g         5756 t
ORIGIN      
        1 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
       61 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      121 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      181 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      241 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      301 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      361 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      421 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      481 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      541 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
      601 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
      661 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
      721 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
      781 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
      841 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
      901 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
      961 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
     1021 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
     1081 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
     1141 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
     1201 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
     1261 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
     1321 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
     1381 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc
     1441 cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct gtaaccttcg
     1501 agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata catggagtaa
     1561 tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt ctatatttgt
     1621 taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga gaccccgttt
     1681 cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat cttctttttt
     1741 acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa acaggtatag
     1801 aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta ttaatgcgat
     1861 gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact ttataaaatt
     1921 tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa agtcgccatg
     1981 agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa acttatccac
     2041 ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat agaaaaatca
     2101 aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt aataaatatc
     2161 ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg tttagtatga
     2221 agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg aaaagcatga
     2281 aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc aaacaagtta
     2341 ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca aaagaagtct
     2401 cgttttctga tatagataac taccttgtca actctttttc tactgttgat acgtacgtca
     2461 ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt gatttattaa
     2521 gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt cattacttta
     2581 tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg actgtttatc
     2641 gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt aaagaatgaa
     2701 aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg gcgacgtgga
     2761 agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt cattacgtcg
     2821 catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac gtggcagaat
     2881 ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc attttttcgt
     2941 ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat tccatcaaag
     3001 catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa
     3061 ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca cgccatgacc
     3121 tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc aggattgctg
     3181 ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga gcgttagaag
     3241 cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt cctgtgagct
     3301 ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta ttataaaact
     3361 atttgatata attataggtg aatattggag ttttcctagc ctataattgg gaaaggagta
     3421 ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac ttctttttca
     3481 gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat ctactatcaa
     3541 tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg aattcggttt
     3601 tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact atggttaaag
     3661 acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta ggttatgatt
     3721 ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa gaagcaagag
     3781 aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat aaggataaaa
     3841 tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca caggtaaagc
     3901 tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat tttgataaaa
     3961 tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa ttatcaaaga
     4021 agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct atggatcgag
     4081 caattgtcct tgggataacc accacaaatc catctaagat tattggcaat gtgaaaaagc
     4141 aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc tctctaattt
     4201 acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta tttatgactg
     4261 gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt gactcaggag
     4321 tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga tttgttgaac
     4381 caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta acttatttat
     4441 ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg agctataacg
     4501 gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct aagctggctg
     4561 gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg ttaatcagta
     4621 tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt gagacgactt
     4681 taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac aaattaaatg
     4741 gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag aatcaattta
     4801 ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc agtaaaaaga
     4861 taagaagagc cagaaaccct tggtataaga ggatttctag ctctcctaca actattccca
     4921 ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac
     4981 ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc
     5041 agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg
     5101 tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat
     5161 atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac
     5221 gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg
     5281 gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac
     5341 ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag
     5401 tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac
     5461 gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt
     5521 tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg
     5581 gtcagataca cctttcaact tgtctaagaa gcgatctctt gcatcgactt tgatgatgtt
     5641 caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag
     5701 accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc
     5761 aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg
     5821 ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca
     5881 gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac
     5941 ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg
     6001 acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc
     6061 atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct
     6121 tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc
     6181 aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt
     6241 gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga
     6301 aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa
     6361 taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact
     6421 accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt
     6481 tattacccct atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat
     6541 attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg
     6601 ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata
     6661 catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat
     6721 tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt
     6781 cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga
     6841 tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct
     6901 gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta
     6961 cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat
     7021 cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg
     7081 gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca
     7141 aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc
     7201 cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca
     7261 aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc
     7321 gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg
     7381 tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt
     7441 agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac
     7501 acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt
     7561 ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac
     7621 tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg
     7681 cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca
     7741 atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct
     7801 gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg
     7861 cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt
     7921 cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga
     7981 acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc
     8041 tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg
     8101 cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg
     8161 agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa
     8221 agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag
     8281 cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga
     8341 gaagttttta aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg
     8401 ttgtttttta cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt
     8461 agataaagga ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg
     8521 ggttttggta cactgatagg gaatttagat agaagggagc caataggatg aagctgacca
     8581 tcagagatat cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata
     8641 acaaaggaag cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac
     8701 atcattataa gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg
     8761 gaatgatcgt ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga
     8821 gctatttgaa tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa
     8881 atgagatcaa gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca
     8941 caccaaatat tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa
     9001 tcattttaat cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat
     9061 atgagggcgc ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca
     9121 tgctgaagga aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg
     9181 cgctgaaaga tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg
     9241 tccaaggtgg atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt
     9301 tttgcgggaa tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa
     9361 aaatcccaga agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga
     9421 ttccgcaatt gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt
     9481 tcctgatcga tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa
     9541 aattaattat tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc
     9601 ttatgtaagc atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac
     9661 taaagtggtt tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca
     9721 ggcaatgcga atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga
     9781 ggaaatcgag tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag
     9841 tgcattattg atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac
     9901 attggcgatt gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt
     9961 agataagcat tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt
    10021 agctagtgtc ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg
    10081 tacactagac aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt
    10141 tgtacgcatt gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc
    10201 ggcggacaaa aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct
    10261 gattgccagt tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga
    10321 tgtgaccact ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa
    10381 gaccatgacg agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat
    10441 gtcagaacct cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac
    10501 attacaagga aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat
    10561 ggttgttctt gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc
    10621 tctggaatca ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga
    10681 cccagcgatc gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc
    10741 tgctaaacaa agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat
    10801 gatgctaggt gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa
    10861 actgcataaa aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag
    10921 taacgacgaa gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga
    10981 atcggcaatg aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga
    11041 ctttaccgtt ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac
    11101 tattttgaaa gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa
    11161 ataccatttt tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt
    11221 acaaggtgag ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc
    11281 cgagacaaaa gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat
    11341 ggtcatcaat atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga
    11401 agcagttgtt gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt
    11461 attagataga gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt
    11521 tgtcaaaaca tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat
    11581 gcgtgaaact gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga
    11641 agaagcaatg gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc
    11701 aatcgtatct ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca
    11761 gaactcaaac aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct
    11821 tattcccatt ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc
    11881 atcaacattg agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc
    11941 aaagcagtat ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc
    12001 gatcctatct ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat
    12061 atgccggtta cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg
    12121 ctgccttatg catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt
    12181 tgaaaaataa aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg
    12241 gtttaggtgc tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct
    12301 ctactgattc atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag
    12361 acacaggtgg tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat
    12421 gggggaaaga tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa
    12481 atgaatcaga ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt
    12541 ttggtattgg ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta
    12601 cgaactttgt tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt
    12661 tcaaagacaa cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa
    12721 acgtagtcgg ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat
    12781 tcaaagccgg tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc
    12841 aatatgctgg tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg
    12901 cacaaaacgc agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag
    12961 aagcaaaatc attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg
    13021 accaatcaga cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat
    13081 ctacactgaa agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta
    13141 aattccctgg cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag
    13201 aaggacaact ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt
    13261 caggtgacgt aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg
    13321 ttagacacat gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa
    13381 gctgccttgt gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag
    13441 aagaacgctc actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga
    13501 tgtgaaaaaa aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa
    13561 tttggaacat tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat
    13621 gcgttgcttg gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta
    13681 ttagagccga catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg
    13741 accaaagcca accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc
    13801 tttactgtaa cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac
    13861 cataaaaaag cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac
    13921 ccagatgctt acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa
    13981 acactttacc gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct
    14041 caagaaattg atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc
    14101 attttgatta cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc
    14161 cgccgtggaa aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc
    14221 gatatgatgg ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa
    14281 gaagtcgttt tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg
    14341 aaaaacttga acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga
    14401 aacgggcagt cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat
    14461 atcaagctaa aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt
    14521 gtaggacacg tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct
    14581 gaaaatatcg cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat
    14641 tattcagtaa tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg
    14701 aatgaacttg ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct
    14761 cgtgaagtcg accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat
    14821 gtaggtgcga tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca
    14881 gtgttactga tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt
    14941 atccatgaag gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta
    15001 ggtctattga tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct
    15061 ggcgcacatg aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt
    15121 tgttttagga ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca
    15181 aggttattct tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt
    15241 aaggcaagca acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg
    15301 gtttttcaat attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt
    15361 ggcacttgcg tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg
    15421 tgccttagct ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac
    15481 aagtgaagtg atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc
    15541 tttacatgaa gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag
    15601 tcaaaatgcc tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa
    15661 ctcaggatta tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac
    15721 gactttagga tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg
    15781 aatgagtagt aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct
    15841 tggcggtgtg gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct
    15901 atctattgga tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat
    15961 tcttttatca gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg
    16021 agcaggtgtt ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg
    16081 gatcaacttc ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga
    16141 gatcgttgcg acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata
    16201 atgtcgacta tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg
    16261 ctaattctaa cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg
    16321 ctagagggaa tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt
    16381 ccatcacttg gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc
    16441 ctattttctc tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc
    16501 gggacagtaa ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat
    16561 ggtaaaggtc aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta
    16621 gcagatattc cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt
    16681 gctattttga ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc
    16741 ctccgttcag ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct
    16801 atgcgttatt caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt
    16861 gctcagtcga tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc
    16921 atggctgcta tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc
    16981 tttggttttg ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt
    17041 ccgccagtat tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc
    17101 ttagggaaag cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct
    17161 tttagcgaat aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta
    17221 aaggcaataa aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg
    17281 tcatggattc agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt
    17341 ctgatacatt aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc
    17401 ttggtttagg aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct
    17461 atgcgacgaa attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa
    17521 tcatgggatt gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc
    17581 tgatcaaaca aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg
    17641 gaacagctgt tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat
    17701 atacttctgc cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag
    17761 aattatatcg gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg
    17821 gccgaatcat tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc
    17881 gccatgatta tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag
    17941 gaaaggacgt gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg
    18001 aatctgtccg cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa
    18061 acgatttttc aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc
    18121 gtcgggatgt agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat
    18181 tgcttgaagc gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc
    18241 cgacgtttac tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa
    18301 aaatgaaaga acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg
    18361 ctgagaactt tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat
    18421 aatcttttta aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac
    18481 ccagtttatc cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg
    18541 attaggtgaa ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc
    18601 gttctttcct gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc
    18661 aggcaagaaa gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca
    18721 aacagtgacc tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac
    18781 taatgcatgt ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga
    18841 tcatatcaat tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc
    18901 tagatttcca gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc
    18961 agcagaaaaa ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac
    19021 ttatgaaacg cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat
    19081 gtcgactgtt ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc
    19141 ttgtattacc aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga
    19201 aacaactcag agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt
    19261 gtaataggag gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg
    19321 ataagatttt acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg
    19381 aagatccagt ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag
    19441 gagaacgtat ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc
    19501 atgaattgat ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat
    19561 tgtcaaaaga cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt
    19621 cgatgatcga aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat
    19681 tactgaaagc atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc
    19741 tttcggaaga ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg
    19801 gcgttttagg agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg
    19861 tgcagacatt aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag
    19921 ctgcagaacg tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag
    19981 cagaataaga aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa
    20041 aatagctcct catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac
    20101 accgtttacc aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca
    20161 aaaa
//
LOCUS       DACEJZ010000243        17914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.065, whole genome shotgun sequence.
ACCESSION   DACEJZ010000243 DACEJZ010000000
VERSION     DACEJZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.065"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            267..1304
                     /locus_tag="IW656_002997"
     CDS             267..1304
                     /locus_tag="IW656_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0838634.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IW656_002998"
     CDS             complement(1393..1716)
                     /locus_tag="IW656_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0838635.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IW656_002999"
     CDS             complement(1703..2410)
                     /locus_tag="IW656_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0838636.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IW656_003000"
     CDS             2496..2837
                     /locus_tag="IW656_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ0838637.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IW656_003001"
     CDS             complement(3008..3856)
                     /locus_tag="IW656_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838638.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IW656_003002"
     CDS             4454..5317
                     /locus_tag="IW656_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0838639.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IW656_003003"
     CDS             5417..5899
                     /locus_tag="IW656_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0838640.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IW656_003004"
     CDS             complement(6162..6629)
                     /locus_tag="IW656_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0838641.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IW656_003005"
     CDS             6752..7603
                     /locus_tag="IW656_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0838642.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IW656_003006"
     CDS             complement(7854..9812)
                     /locus_tag="IW656_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0838643.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IW656_003007"
     CDS             complement(9809..10681)
                     /locus_tag="IW656_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0838644.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IW656_003008"
     CDS             complement(11023..11895)
                     /locus_tag="IW656_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0838645.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IW656_003009"
     CDS             12023..12592
                     /locus_tag="IW656_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0838646.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IW656_003010"
     CDS             12659..13396
                     /locus_tag="IW656_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0838647.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IW656_003011"
     CDS             13627..14895
                     /locus_tag="IW656_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0838648.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IW656_003012"
     CDS             15382..15846
                     /locus_tag="IW656_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0838649.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IW656_003013"
     CDS             15830..16459
                     /locus_tag="IW656_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0838650.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..17709
                     /locus_tag="IW656_003014"
                     /pseudo
     CDS             16813..17709
                     /locus_tag="IW656_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(17730..>17914)
                     /locus_tag="IW656_003015"
     CDS             complement(17730..>17914)
                     /locus_tag="IW656_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ0838651.1"
                     /translation="MIGAEKWIELIKEYSVPKELTAPLLNAMIEKILIHEATTNEENE
                     RIQEIEIYYRFIGKVD"
BASE COUNT         5810 a         3220 c         3647 g         5237 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccagttt
    17701 cccttagtta aagatatgaa ccctctttat cagtctactt ttccaataaa tcggtagtat
    17761 atctctattt cctgtattct ttcgttctcc tcattcgttg ttgcttcgtg aataaggatt
    17821 ttttctatca ttgcatttaa taacggtgct gtcagttcct ttggtacgga atactccttg
    17881 attaactcaa tccacttttc agcacctatc atat
//
LOCUS       DACEJZ010000244         8062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.117, whole genome shotgun sequence.
ACCESSION   DACEJZ010000244 DACEJZ010000000
VERSION     DACEJZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.117"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW656_003016"
     CDS             128..1564
                     /locus_tag="IW656_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0838652.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW656_003017"
     CDS             1753..4200
                     /locus_tag="IW656_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0838653.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW656_003018"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW656_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0838654.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW656_003019"
     CDS             complement(5690..6793)
                     /locus_tag="IW656_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0838655.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW656_003020"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW656_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0838656.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DACEJZ010000245         7482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.122, whole genome shotgun sequence.
ACCESSION   DACEJZ010000245 DACEJZ010000000
VERSION     DACEJZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.122"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW656_003021"
     CDS             109..192
                     /locus_tag="IW656_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ0838657.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW656_003022"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW656_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0838658.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW656_003023"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW656_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW656_003024"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW656_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW656_003025"
     CDS             2348..2593
                     /locus_tag="IW656_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0838659.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW656_003026"
     CDS             2696..3565
                     /locus_tag="IW656_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ0838660.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW656_003027"
     CDS             3546..4280
                     /locus_tag="IW656_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0838661.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW656_003028"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW656_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW656_003029"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW656_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ0838662.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW656_003030"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW656_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0838663.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IW656_003031"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IW656_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IW656_003032"
     CDS             6927..7469
                     /locus_tag="IW656_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0838664.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACEJZ010000246         6127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.134, whole genome shotgun sequence.
ACCESSION   DACEJZ010000246 DACEJZ010000000
VERSION     DACEJZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.134"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IW656_003033"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IW656_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0838665.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IW656_003034"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IW656_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0838666.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="IW656_003035"
     CDS             3976..5238
                     /locus_tag="IW656_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0838667.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..>6127
                     /locus_tag="IW656_003036"
     CDS             5483..>6127
                     /locus_tag="IW656_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0838668.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN"
BASE COUNT         2110 a          876 c         1264 g         1877 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaat
//
LOCUS       DACEJZ010000247         6127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.135, whole genome shotgun sequence.
ACCESSION   DACEJZ010000247 DACEJZ010000000
VERSION     DACEJZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.135"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IW656_003037"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IW656_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0838669.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IW656_003038"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IW656_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0838670.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="IW656_003039"
     CDS             3976..5238
                     /locus_tag="IW656_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0838671.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..>6127
                     /locus_tag="IW656_003040"
     CDS             5483..>6127
                     /locus_tag="IW656_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0838672.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN"
BASE COUNT         2109 a          876 c         1265 g         1877 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaat
//
LOCUS       DACEJZ010000248         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.145, whole genome shotgun sequence.
ACCESSION   DACEJZ010000248 DACEJZ010000000
VERSION     DACEJZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.145"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW656_003041"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW656_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW656_003042"
     CDS             2326..2901
                     /locus_tag="IW656_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838673.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW656_003043"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW656_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0838674.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW656_003044"
     CDS             3788..4942
                     /locus_tag="IW656_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0838675.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACEJZ010000249         4863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.146, whole genome shotgun sequence.
ACCESSION   DACEJZ010000249 DACEJZ010000000
VERSION     DACEJZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.146"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            362..1273
                     /locus_tag="IW656_003045"
     CDS             362..1273
                     /locus_tag="IW656_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ0838676.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1426..1911
                     /gene="vanZ"
                     /locus_tag="IW656_003046"
     CDS             1426..1911
                     /gene="vanZ"
                     /locus_tag="IW656_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ0838677.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2198..2527)
                     /locus_tag="IW656_003047"
     CDS             complement(2198..2527)
                     /locus_tag="IW656_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0838678.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2832..3161
                     /locus_tag="IW656_003048"
     CDS             2832..3161
                     /locus_tag="IW656_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0838679.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3151..3438
                     /locus_tag="IW656_003049"
     CDS             3151..3438
                     /locus_tag="IW656_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0838680.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3717..4280)
                     /locus_tag="IW656_003050"
     CDS             complement(3717..4280)
                     /locus_tag="IW656_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0838681.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1705 a          734 c          837 g         1587 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccattga agatgtaagg cagagcgata
       61 ttgcggtcat tatctgcgtg cgctgcggca agatagcctg ataataagac tgatcgcata
      121 gaggggtggt atttcacacc gcccattgtc aacaggcagt tcagcctcgt taaattcagc
      181 atgggtatca cttatgaaaa ttcatctaca ttggtgataa tagtaaatcc agtagggcga
      241 aataattgac tgtaatttac ggggcaaaac ggcacaatct caaacgagat tgtgccgttt
      301 aaggggaaga ttctagaaat atttcatact tccaactata tagttaagga ggagactgaa
      361 aatgaagaag ttgttttttt tattgttatt gttattctta atatacttag gttatgacta
      421 cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc
      481 caaagaacat ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga
      541 agaacaggtt tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga
      601 aagtgtgaag tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg
      661 gttgcttgat agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt
      721 caatgatgct gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt
      781 tgatgagcaa agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta
      841 tagtgagcat aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg
      901 agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta
      961 tccagaggac aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt
     1021 tggtttacca catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga
     1081 ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt
     1141 ttattatcct gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat
     1201 atcaggaaac aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa
     1261 ttcaaggagg taaggatggc ggaatgaaac caacgaaatt aatgaacagc attattgtac
     1321 tagcactttt ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc
     1381 tatactaatg atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta
     1441 tctagaggat tgctagcttt atatttagtg acactaatct ggttagtgtt attcaaatta
     1501 caatacaata ttttatcagt atttaattat catcaaagaa gtcttaactt gactccattt
     1561 actgctactg ggaatttcag agagatgata gataatgtta taatctttat tccatttggc
     1621 ttgcttttga atgtcaattt taaagaaatc ggatttttac ctaagtttgc ttttgtactg
     1681 gttttaagtc ttacttttga aataattcaa tttatcttcg ctattggagc gacagacata
     1741 acagatgtaa ttacaaatac tgttggaggc tttcttggac tgaaattata tggtttaagc
     1801 aataagcata tgaatcaaaa aaaattagac agagttatta tttttgtagg tatacttttg
     1861 ctcgtattat tgctcgttta ccgtacccat ttaagaataa attacgtgta agatgtctaa
     1921 atcaagcaat ctgatctttc atacacataa agatattgaa tgaattggat tagatggaaa
     1981 acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta
     2041 aaaagcttac ctaacactat agtaacaaag aaagcccaat tatcaatttt agtgctgagg
     2101 aattggtctc tttaataaat ttccttaacg ttgtaaatcc gcattttcct gacggtaccc
     2161 ccaaactaag atttcataga tagattttct gataacttta aaaaacacga atagcggaaa
     2221 ggatgtcttc attcgctaaa gagtaataaa ctacttttcc atcttgacgt gattttgcca
     2281 ttccatgttt ttttaaaaaa cgtaaatggt gtgacgttgt agcgacacta gcagtaagta
     2341 tttctgctaa atcacagacg cacatttcct tataggtttc taaagcatag aaaattttaa
     2401 ttctagaaaa atcgctgaaa catttcccta acacaagtaa gtttgaaaaa tctttttgat
     2461 ccaatttatt ttttattcca tttacttttt ctttatgtac gacagtcact tggcaaactt
     2521 ttttcattca tctcacctca cattcaaacg attatttgaa tgaagtatac ctatttgatc
     2581 actcttttgt caatctacct taattccaat acactattat aagcaatttc atgtattgaa
     2641 gtaaacttac ttgcaagtgc gtttttgatt ccaagttaaa aacatattat tattctacat
     2701 aatttcctaa tcaaataaaa ctcctttgaa aattgttcta aaggagtttt attattttgt
     2761 atttctacta aaaatagcgt acaatgtaat tactaagtaa ttacgaaatg taaaataatg
     2821 gaggaaataa tatgattaca aaaacaagaa aacaaggaaa ttctattatg ttaacggtcc
     2881 ctaaagaatt cgatgtgccc aatggtgttg aagtagaagc taagcttgta gagaatggta
     2941 tcctttatga gtttgtagag cctaaaaaag aattttttga ttttagtgaa gatattttgg
     3001 ctgatattct ttcagaaggt tatagcaaac aagagatctt aaaagaattt agaaatagaa
     3061 aaaatgcgtt aacttctgca tttaaatcta ttgctgaaga tactgttgca aattctaaac
     3121 cgatgacaaa agaggagttg gcagcagaaa ttggcttatg agattctacc ttctaaacac
     3181 gtaataaagt atctaaaaaa attaaaagag aaaacattaa aagaacaatt tttaacgata
     3241 atttatgatg agatagcagt tcgtcctcat tcgggagaac aaaaaacggg tgatttatca
     3301 ggaatttggg ctattggatt caagtatgca ggtacgacat atcgcgttgc atatgaaatt
     3361 aaagataatg cagtgatacc tatcttacta tgtggaacgc atgaaaattt ttatgaacaa
     3421 ttaaaaaaga ttaggtaaga acccaatgtt tcaacttgag aggatagacg acactttaaa
     3481 agataatttt taacagctga gagttgtaat accctataat tttaaaccac taacctatat
     3541 atttatgaaa aatagaaaaa attaaaacgc attttaagcc tctttcaaat taaaagcttc
     3601 atccttatgc acaaagaggg cttaaaatgc gttattttat attgtttttt agtaataata
     3661 aaaaaatcac tctaattctc ccataaatat tatagattcc taaagattaa aatagattat
     3721 tccctattgt cttttaagta gcggtagagt gttgccttgc taataccagt catttccgta
     3781 atttctttta cgctataaac ctgactttta tacaatttta ctgctcgatc aacatctttc
     3841 tggttcactc gtggacggcc acctttacga cctctcgcac gtgcactttt caaaccttct
     3901 ttggtacgtt caacaattaa atcacgttcg aactgactaa atgcttggaa gacagtcatc
     3961 attaagcgtc cgtgaggcgt gctggtatcg aagttttctt tcaagctcac cagcttaaca
     4021 tggtgttctt caaaataggt aacaaggtca atcaaatcct ttgtactacg acccaatcga
     4081 gaaaaacttt ctacaattac cgtatctccc ggacgaattt tatcttttaa acgatttagt
     4141 tggggacgaa ccgctttggt tcccgtcatt ttctcagtta ggatttcatt acaattttgt
     4201 tcattgagta agtctaattg acgatttagt tcttgtgaac gtgtactcac tcgggcataa
     4261 ccataaatgt atccactcat gcttttcttc ctttcgtact ttaattctgg cttgattgta
     4321 tcataaaagg gagttataga tacatagtta atgcaacaag ttatgataca aaatatgacc
     4381 tcttttattt aataaaagag gtcatttcat tttgtctcat aatcgattgt ttttgatacg
     4441 agtgtgtata cgaaataaac aaaaaaatct taagtaaatt tctagatacc taatttaatc
     4501 ctaactagtg tatcctaaag ttaataacga gtttatcaca atgaaaatta taaaatgctc
     4561 ccatattaga tataacatgc aatatttgaa ttagaactac aaaatttatt cgttttataa
     4621 agtaaataat gttgagatac taaggaatag aatattataa ggaattgagc tgaatgtatt
     4681 ggataaaaaa agaataactt tattagagat ggactataaa aatgctcgta attttcttct
     4741 gaagtctgaa agctatttga atgctcagct accagaatat attagttttg acgaaacttt
     4801 aaaatctgct aaaaaagttt taacttcgcc gtttgtataa ttaagcgaga caaataaaaa
     4861 agc
//
LOCUS       DACEJZ010000250         4285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.154, whole genome shotgun sequence.
ACCESSION   DACEJZ010000250 DACEJZ010000000
VERSION     DACEJZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.154"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            260..1474
                     /locus_tag="IW656_003051"
     CDS             260..1474
                     /locus_tag="IW656_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0838682.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1505..1723
                     /locus_tag="IW656_003052"
                     /pseudo
     CDS             1505..1723
                     /locus_tag="IW656_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2142..2615
                     /gene="dfrF"
                     /locus_tag="IW656_003053"
     CDS             2142..2615
                     /gene="dfrF"
                     /locus_tag="IW656_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ0838683.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2742..3398
                     /locus_tag="IW656_003054"
     CDS             2742..3398
                     /locus_tag="IW656_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0838684.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3580..4110
                     /locus_tag="IW656_003055"
     CDS             3580..4110
                     /locus_tag="IW656_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0838685.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1421 a          628 c          916 g         1320 t
ORIGIN      
        1 attttatatg aagtattctt tattccttat gaaaaaatat ctttaaaaat aaaaaaagac
       61 gaaaataaac ggttttctat aaaaagaaga ccgtttattt tttgtcttta tttgtgtgaa
      121 aaacgaaaaa gagcacaaca catcaagctt ttattaattg tctgtctttt ataaatgcaa
      181 gtattaattc aaaaaaacag aagaaaaaaa caactacacc ttcttttata ctcgtaatcg
      241 tagaaaggag gcgaaaaaaa tgaatacatt acctatcaat acaggagata cagcattcat
      301 gattttatgt acagcaatgg tttgcctgat gactcctggt ctagcttttt tttacggcgg
      361 attagcacgg aagaataata tactaacgat catgtctcaa tcttttctct ccgttgggat
      421 aacaacgatt atgtgggtat ttggtggatt cggattggcc tttggacggg atatcggcgg
      481 tgttattgga aacccggcag atttcttttt gatgagacat gttacaagct tccccaacat
      541 gtttcatggt gcaacgattc catttttaat gttctttctt taccaactga tgtttatcgt
      601 gattaccgtt cctttgatga caggagcttt tgcagggaga ttgaatttaa aaggctatat
      661 tatacttgtt attttttgga atctgctgat ttatttccct gtttgccact ggatatgggg
      721 tggcggattt ttggatcaga tgggatttag agatttcgct ggtggagcag tcatccacac
      781 gacagctgga ttcggctctt tagcatgtat cctcacactt ggtcaaagaa agattccttt
      841 aggagaaaga agtcagcata gcagtcttat ggcagctgct atcggtacag gactgctttg
      901 gtttggctgg ttcggtttta attccggtgg tgcacttcga gcagatgaac aagcagtcaa
      961 tgcatttggt tcaacattca tcgctcttgc atttgcgatg attacttggc tgatcattgc
     1021 aaaagtaaaa ggaaatggct ttgattttgt cgacgtacta acaggatccg ttgctggatt
     1081 ggcagccatt acgccttgtg ccggttatgt agaagcaaaa agtgccatgc tgattgggat
     1141 cattgcgggt atcgtctgtc atgctgctgt tgattttcgt aaaaagaaac aatgggatga
     1201 tgcacttgat gtgtggggtg ttcatggcat gggcggattc acaggtacga ttctgatagg
     1261 gatctttgca tctaacagcc atttactaac gaatccaagc agctggtact tcttagggat
     1321 tcaagtgatt ggtgtcatca ttactgccgt gtatgcctat gtgctgacaa cagtgatttt
     1381 aaaatcggcg atgcacttta caacgatcac tactacaaaa gaagaacaag aagaaggatt
     1441 ggactttttc ttctttggca acaaactcaa ttgaaacaaa ttcaaataga aaagaggaag
     1501 attcatgcaa caaagcaaaa cgaaaattcc tgcaaaaaaa ttaacgttct ttggcttttt
     1561 ggctatgacc atctcaatgg tcgtttcatt atatgaatat cccacattcg cgacctcagg
     1621 cttcagctta gtatttttct tgttattagg cggtttgcta tggtttatcc ctgtggcatt
     1681 gtgcgcagca gagatggcaa cggtgaaagg atgggaaaaa ggtgtttccc aaacaaaaag
     1741 atattaagaa acagcccatg tttacctgct tgcaaacaaa agaatattgc tactagacta
     1801 agagatacta cataagtgtg ttggtatctc ttttttgttg ccgtgaaaca gcagactatt
     1861 tcatttctgc ttgattacct taaaacttat tagtctatgg tttgtcaagg gcttgttgcg
     1921 tgagaaatgc tgaatatcac cctttacaaa acataggctg ataagtaact tttcaaaagc
     1981 agaaaagaaa tggcaatgct tataacaata tgatataatg cagatgaaga caaaaaagga
     2041 atttgtcgag ctggttagtt cgagataatt ttgagtttgt taagatgtgg tgcacttgat
     2101 ggaatcacag gtacgacatc atattatgga ggatgaaata gatgataggt ttgattgttg
     2161 cgaggtcaaa gaataatgtt ataggcaaga atggtaatat accatggaaa ataaagggag
     2221 aacaaaagca atttagagag ttaacaacgg gtaatgtggt tattatgggg cgaaagtctt
     2281 atgaagaaat cggtcatccg ttgcctaata gaatgaatat tgttgtttcc accacaacag
     2341 agtatcaagg agataattta gtttcagtta aatcattaga agatgcatta ttattggcta
     2401 aaggacgaga tgtatacata tctggtggat atggactatt taaggaagct ttgcaaatag
     2461 tagataaaat gtatatcaca gaagtagatt taaatattga agatggagat acattctttc
     2521 cagaatttga tatcaatgat tttgaagttt tgatagggga aacacttggt gaggaagtga
     2581 aatatacgag aacattttat gtaaggaaaa aatgaattga gtaaattttg gatttagggt
     2641 gttttcataa atatattgct ttttatgcat atataaattg ttgtaagaca aattccagtt
     2701 tgtatggggc gataaaatca gaatttgaga gaggattgaa tatgaataag gaatggtctg
     2761 aacttaataa aacaatgcag gcacaaataa aaaagaaaga tacttataag aggggtattg
     2821 atactttact tactttacga agtcagttaa tacaaacctt agtatctttc aaagaagaat
     2881 tatgcagaga agattttaac tcaatcccct tcataaacgc tgatggttat catagtaaga
     2941 cgattgctta ttctatttgg catattttcc gcattgaaga tatagttgtg catacagtga
     3001 taaatgaaga tgaacaagta ttttttgcag gcaattatca agagcgtatc aattcaccta
     3061 tcattacaac aggaaatgaa ctcatgaaac agcagattgc agacttttca aaacagctta
     3121 atctggaaga attatattta tatattttcg aagtatggga aagcactgag aaaatgcttg
     3181 aacggttatc ttatgatgaa ttgaaaagaa aaataccgaa agaaagaaaa ggatacttag
     3241 aatcgttgaa tgtagtaaac gacaatgaaa aagcaatttg gctgattgat tattggtgta
     3301 ataaagatat ttgtggacta attcaaatgc cgttttcaag acattggatt atgcacacag
     3361 aagcctgttt gcgtataaag aacaagatac attcataggc aaaaatttca gtttgtagaa
     3421 ctaaggaaat tggaatttaa ggaggaataa agtatggtta gtgattttat aagagcagca
     3481 tttccatgga ttttaatggg tttatttgta gcaatcagtt gttcccttat gagtaaaaaa
     3541 gagaaataaa ttcctgtttg tatttataaa agagaaaaaa tgagaaaagt agttttattt
     3601 attgccatga gccttgacgg atatattgcg gacggtaatg gtggagtagc ttggctaaat
     3661 ggtcatggaa atgacaatga aaacatcgac acttatacag aatttaccaa agatatagat
     3721 actgtcttaa tggggtggaa tacttctcat caggttgtta ctgaactttc tccgcaagaa
     3781 tgggtatata ataaatttac aacttatgta ttaacacata aagagtgcaa ttcttcccaa
     3841 caaattcatt ttacaagtga aaatcctgtt ttattattgg aacggctaaa acaagaagca
     3901 ggaaaggata tttggatttg tggcggagca aatcttgttc agcagttggt aagcaaaaat
     3961 atgattgata aatattacat ttctgttatt cctactctgt taggaaatgg ggttcgtctt
     4021 ttgggcaaca tagacaggga aataaagtta aggctttgta aaacacagac ttataacgga
     4081 ataaccgatt tgatttatat gcgtagataa ctagagagaa acagaaaagc tgttaaaccg
     4141 acggagcgat ccgaccacgt tggcgggaaa atccttaaga tttttccgcc atgcttgcaa
     4201 gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc gttcaaagcc cccgttatct
     4261 aacaaggggg gcacaaaaaa caaga
//
LOCUS       DACEJZ010000251         3831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.161, whole genome shotgun sequence.
ACCESSION   DACEJZ010000251 DACEJZ010000000
VERSION     DACEJZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.161"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1729
                     /locus_tag="IW656_003056"
     CDS             <1..1729
                     /locus_tag="IW656_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0838686.1"
                     /translation="DSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPL
                     IQTVRAYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISEL
                     DKKISTLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLM
                     VTHDRYFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQE
                     LAWMRAGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGS
                     YRINDQTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHL
                     AYYTQENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKL
                     SGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYF
                     LDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSY
                     MEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARW
                     EYLSEWVED"
     gene            1741..2688
                     /locus_tag="IW656_003057"
     CDS             1741..2688
                     /locus_tag="IW656_003057"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0838687.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2708..3235
                     /locus_tag="IW656_003058"
     CDS             2708..3235
                     /locus_tag="IW656_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0838688.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3298..>3831)
                     /locus_tag="IW656_003059"
     CDS             complement(3298..>3831)
                     /locus_tag="IW656_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0838689.1"
                     /translation="AHLHSSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVF
                     DHSSIFLLIAGSYTPFCLLSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVS
                     TIIYIFMGWLCVVAAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHL
                     FVMLGAGFMYFSVLFFT"
BASE COUNT         1361 a          674 c          827 g          969 t
ORIGIN      
        1 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
       61 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
      121 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
      181 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
      241 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
      301 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
      361 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
      421 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
      481 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
      541 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
      601 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
      661 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
      721 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
      781 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
      841 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
      901 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
      961 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     1021 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     1081 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     1141 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     1201 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     1261 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     1321 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     1381 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     1441 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     1501 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     1561 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     1621 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     1681 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     1741 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     1801 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     1861 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     1921 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     1981 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     2041 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     2101 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     2161 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     2221 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     2281 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     2341 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     2401 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     2461 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     2521 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     2581 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     2641 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     2701 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     2761 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     2821 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     2881 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     2941 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     3001 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     3061 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     3121 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     3181 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     3241 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     3301 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     3361 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     3421 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     3481 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     3541 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     3601 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     3661 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     3721 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     3781 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg c
//
LOCUS       DACEJZ010000252         3604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.168, whole genome shotgun sequence.
ACCESSION   DACEJZ010000252 DACEJZ010000000
VERSION     DACEJZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.168"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..164)
                     /locus_tag="IW656_003060"
     CDS             complement(<1..164)
                     /locus_tag="IW656_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0838690.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IW656_003061"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IW656_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0838691.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="IW656_003062"
     CDS             complement(963..2915)
                     /locus_tag="IW656_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0838692.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3218)
                     /locus_tag="IW656_003063"
     CDS             complement(2922..3218)
                     /locus_tag="IW656_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0838693.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3393..>3604)
                     /locus_tag="IW656_003064"
     CDS             complement(3393..>3604)
                     /locus_tag="IW656_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0838694.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1115 a          622 c          583 g         1284 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tctttttttt
     3421 gtatattcaa ttttgaaaac atttccgcaa agttcagacg gacctcgatg tgttgatgaa
     3481 taaaatttat tagtaataac ttctataagg ttatcaatcc ctatattact ttttgcactt
     3541 ccatgataaa gagggaacag agaacaattc tgaaatctta tgctttcctc ttgttcgagt
     3601 tcca
//
LOCUS       DACEJZ010000253         3000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.172, whole genome shotgun sequence.
ACCESSION   DACEJZ010000253 DACEJZ010000000
VERSION     DACEJZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.172"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW656_003065"
     CDS             245..1213
                     /locus_tag="IW656_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0838695.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW656_003066"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW656_003066"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0838696.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW656_003067"
     CDS             2243..2851
                     /locus_tag="IW656_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0838697.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          722 g          810 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccctt
//
LOCUS       DACEJZ010000254         1650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E399
            SAMN07141477-rid9870703.guided.198, whole genome shotgun sequence.
ACCESSION   DACEJZ010000254 DACEJZ010000000
VERSION     DACEJZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141477
            Sequence Read Archive: SRR5811547
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740877.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740877.1
            Genome Coverage       :: 61x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 03:20:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,069
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,961
            CDSs (with protein)               :: 2,961
            Genes (RNA)                       :: 36
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 29 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 16 of 72
            Pseudo Genes (multiple problems)  :: 12 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141477-rid9870703.guided.198"
                     /isolate="E399"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2012"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1348
                     /locus_tag="IW656_003068"
     CDS             <1..1348
                     /locus_tag="IW656_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0838698.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..>1650)
                     /locus_tag="IW656_003069"
     CDS             complement(1478..>1650)
                     /locus_tag="IW656_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0838699.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          671 a          148 c          278 g          553 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta
//
